1. A mono-allelic bivalent chromatin domain controls tissue-specific imprinting at Grb10
- Author
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Stormy J. Chamberlain, Jean-Philippe Hugnot, Terry Magnuson, Robert Feil, Lionel A. Sanz, Jean Charles Sabourin, Amandine Henckel, Philippe Arnaud, Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Department of Genetics, University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC)-University of North Carolina System (UNC)-Carolina Center for the Genome Sciences, Physiopathologie et thérapie des déficits sensoriels et moteurs, Université Montpellier 2 - Sciences et Techniques (UM2)-IFR76-Institut National de la Santé et de la Recherche Médicale (INSERM), Hamel, Christian, Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), The University of North Carolina at Chapel Hill-Carolina Center for the Genome Sciences, Université Montpellier 2 - Sciences et Techniques ( UM2 ) -IFR76-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
- Subjects
Male ,Cellular differentiation ,GRB10 Adaptor Protein ,MESH: Neurons ,MESH : Promoter Regions, Genetic ,MESH: Mice, Knockout ,Histones ,Mice ,0302 clinical medicine ,MESH : Embryo, Mammalian ,MESH : Female ,MESH: Animals ,Imprinting (psychology) ,Promoter Regions, Genetic ,MESH: CpG Islands ,Cells, Cultured ,Genetics ,Mice, Knockout ,Neurons ,MESH: Histones ,0303 health sciences ,biology ,MESH : Chromatin ,General Neuroscience ,Stem Cells ,Polycomb Repressive Complex 2 ,Brain ,MESH : GRB10 Adaptor Protein ,Cell Differentiation ,MESH : CpG Islands ,Chromatin ,Histone ,MESH: Repressor Proteins ,MESH : Stem Cells ,Female ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,MESH : Repressor Proteins ,MESH : Cell Differentiation ,Bivalent chromatin ,MESH: Cells, Cultured ,MESH: Cell Differentiation ,MESH : Male ,MESH : Mice, Inbred C57BL ,MESH: Stem Cells ,General Biochemistry, Genetics and Molecular Biology ,Bivalent (genetics) ,Article ,MESH : Neurons ,MESH: Chromatin ,03 medical and health sciences ,Genomic Imprinting ,MESH: Brain ,MESH: Mice, Inbred C57BL ,MESH : Mice ,MESH : Cells, Cultured ,MESH: Promoter Regions, Genetic ,Animals ,MESH : Histones ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Molecular Biology ,Gene ,MESH: Mice ,Alleles ,030304 developmental biology ,General Immunology and Microbiology ,MESH: Alleles ,MESH: Embryo, Mammalian ,MESH : Genomic Imprinting ,Embryo, Mammalian ,MESH: Male ,MESH: Genomic Imprinting ,Mice, Inbred C57BL ,Repressor Proteins ,MESH: GRB10 Adaptor Protein ,MESH : Brain ,[ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,biology.protein ,MESH : Mice, Knockout ,CpG Islands ,MESH : Animals ,Genomic imprinting ,MESH : Alleles ,MESH: Female ,030217 neurology & neurosurgery - Abstract
International audience; Genomic imprinting is a developmental mechanism that mediates parent-of-origin-specific expression in a subset of genes. How the tissue specificity of imprinted gene expression is controlled remains poorly understood. As a model to address this question, we studied Grb10, a gene that displays brain-specific expression from the paternal chromosome. Here, we show in the mouse that the paternal promoter region is marked by allelic bivalent chromatin enriched in both H3K4me2 and H3K27me3, from early embryonic stages onwards. This is maintained in all somatic tissues, but brain. The bivalent domain is resolved upon neural commitment, during the developmental window in which paternal expression is activated. Our data indicate that bivalent chromatin, in combination with neuronal factors, controls the paternal expression of Grb10 in brain. This finding highlights a novel mechanism to control tissue-specific imprinting.
- Published
- 2008