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Your search keyword '"Vangansewinkel, Tim"' showing total 4 results
Start Over Author "Vangansewinkel, Tim" Topic mesenchymal stem cells
4 results on '"Vangansewinkel, Tim"'

1. Cell-Based Delivery of Interleukin-13 Directs Alternative Activation of Macrophages Resulting in Improved Functional Outcome after Spinal Cord Injury.

Author

Dooley D, Lemmens E, Vangansewinkel T, Le Blon D, Hoornaert C, Ponsaerts P, and Hendrix S

Subjects
Animals, Axons drug effects, Axons pathology, CD4-Positive T-Lymphocytes drug effects, CD4-Positive T-Lymphocytes immunology, Cell Count, Demyelinating Diseases pathology, Demyelinating Diseases physiopathology, Interleukin-13 pharmacology, Interleukin-13 therapeutic use, Macrophages drug effects, Macrophages pathology, Mice, Inbred BALB C, Mice, Inbred C57BL, Microglia drug effects, Microglia pathology, Neuroprotective Agents pharmacology, Neuroprotective Agents therapeutic use, Recovery of Function drug effects, Spinal Cord Injuries immunology, Spinal Cord Injuries pathology, Treatment Outcome, Interleukin-13 administration & dosage, Macrophage Activation drug effects, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells metabolism, Spinal Cord Injuries physiopathology, Spinal Cord Injuries therapy
Abstract

The therapeutic effects of mesenchymal stem cell (MSC) transplantation following spinal cord injury (SCI) to date have been limited. Therefore, we aimed to enhance the immunomodulatory properties of MSCs via continuous secretion of the anti-inflammatory cytokine interleukin-13 (IL-13). By using MSCs as carriers of IL-13 (MSC/IL-13), we investigated their therapeutic potential, compared with non-engineered MSCs, in a mouse model of SCI. We show that transplanted MSC/IL-13 significantly improve functional recovery following SCI, and also decrease lesion size and demyelinated area by more than 40%. Further histological analyses in CX 3 CR1 EGFP/+ CCR2 RFP/+ transgenic mice indicated that MSC/IL-13 significantly decrease the number of resident microglia and increase the number of alternatively activated macrophages. In addition, the number of macrophage-axon contacts in MSC/IL-13-treated mice was decreased by 50%, suggesting a reduction in axonal dieback. Our data provide evidence that transplantation of MSC/IL-13 leads to improved functional and histopathological recovery in a mouse model of SCI., (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2016
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2. Cryopreservation and Banking of Dental Stem Cells

Author

Hilkens, Petra, Driesen, Ronald B., Wolfs, Esther, Gervois, Pascal, Vangansewinkel, Tim, Ratajczak, Jessica, Dillen, Yörg, Bronckaers, Annelies, Lambrichts, Ivo, Karimi-Busheri, Feridoun, editor, and Weinfeld, Michael, editor

Published
2016
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3. Stem Cell-Based Therapies for Ischemic Stroke: Preclinical Results and the Potential of Imaging-Assisted Evaluation of Donor Cell Fate and Mechanisms of Brain Regeneration

Author

Gervois, Pascal, Wolfs, Esther, Ratajczak, Jessica, Dillen, Yörg, Vangansewinkel, Tim, Hilkens, Petra, Bronckaers, Annelies, Lambrichts, Ivo, and Struys, Tom

Subjects
ischemic stroke, mesenchymal stem cells, induced pluripotent stem cells, mechanisms of stem cell therapy, noninvasive imaging
Abstract

Stroke is the second most common cause of death and is a major cause of permanent disability. Given the current demographic trend of an ageing population and associated increased risk, the prevalence of and socioeconomic burden caused by stroke will continue to rise. Current therapies are unable to sufficiently ameliorate the disease outcome and are not applicable to all patients. Therefore, strategies such as cell-based therapies with mesenchymal stem cell (MSC) or induced pluripotent stem cell (iPSC) pave the way for new treatment options for stroke. These cells showed great preclinical promise despite the fact that the precise mechanism of action and the optimal administration route are unknown. To gain dynamic insights into the underlying repair processes after stem cell engraftment, noninvasive imaging modalities were developed to provide detailed spatial and functional information on the donor cell fate and host microenvironment. This review will focus on MSCs and iPSCs as types of widely used stem cell sources in current (bio)medical research and compare their efficacy and potential to ameliorate the disease outcome in animal stroke models. In addition, novel noninvasive imaging strategies allowing temporospatial in vivo tracking of transplanted cells and coinciding evaluation of neuronal repair following stroke will be discussed. Esther Wolfs, Jessica Ratajczak, Tim Vangansewinkel, Petra Hilkens, and Annelies Bronckaers are funded by Fonds Wetenschappelijk Onderzoek by grants G0A7514N, G089213N, G029112N, 12D8516N, and 1508015N, respectively. Yorg Dillen is funded by Bijzonder On- ¨ derzoeksfonds by grant BOF15DOC04.

Published
2016

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