Search

The probable positive effects of photobiomodulation therapy (PBMT) and oxytocin (OT) treatments together or alone were evaluated on cell viability along with the changes in the gene expression of Osteocalcin (OC), Osteoprotegerin (OPG), and Runt-related transcription factor 2 (Runx2) levels of sham (healthy)-Bone marrow mesenchymal stem cell(BMMSC) and ovariectomy-induced osteoporosis (OVX)-BMMSC. BMMSC was harvested from healthy and OVX rats and was cultured in osteogenic induction medium (OIM). There were five groups of BMMSCs: (1) sham -BMMSCs; (2) control -OVX-BMMSCs; (3) OT-treated-OVX-BMMSCs; (4) PBMT-treated-OVX-BMMSCs, and (5) OT + PBMT-OVX-BMMSCs. In all 5 groups, BMMSC viability and proliferation as well as gene expression of OC, OPG, and RUNX2 were evaluated. PBMT and PBMT + OT treatments showed a promising effect on the increased viability of OVX-BMMSC (ANOVA test; LSD test, p = 0.01, p = 0.002). The results of gene expression analysis revealed that the sham- BMMSCs responded optimally to OT treatment. It was also found that OVX-BMMSCs responded optimally to PBMT + OT and PBMT treatments at early and middle stages of osteogenic induction process. Nevertheless, they responded optimally to PBMT + OT and OT especially at the late stage of osteogenic induction process. PBMT and PBMT + OT treatments significantly increased viability of OVX-BMMSC in OIM in vitro. Both PBMT and PBMT + OT treatments could promote mineralization of OVX-BMMSC in the culture medium at early and middle stages of osteogenic induction process. Both OT and PBMT + OT treatments could promote mineralization of OVX-BMMSC in vitro at late stages of osteogenic induction process.

Osteoporotic bone marrow mesenchymal stem cells (BMMSCs) are involved in the pathogenesis of osteoporosis (OP). Photobiomodulation (PBM) has positive effects on healthy BMMSCs. The goal of current experiment was to evaluate the combined influence of photobiomodulation PBM and alendronate (ALN) incubation on ovariectomized induced osteoporosis(OVX)- BMMSC viability in vitro. 15 female adult Wistar rats were distributed into the 2 groups: (1) 3 healthy (sham)control rats, (2) 12 OVX- rats. All OVX rats underwent ovariectomy. After 3.5 months sham and OVX rats were euthanized and their MSC harvested and cultured in a complete osteogenic incubation medium (OM). As the next step, in sham and OVX groups flowcytometry and osteogenic differentiation assays were performed. OVX- rats were divided into (2) OVX-control, (3) OVX- PBM (HeNe laser, 623.8 nm, 1.2 J/cm 2 , one time), (4) OVX-ALN (10 -8  M, three times incubations), and (5) OVX-PBM + ALN, Finally BMMSC viability of all five groups were evaluated using MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) assay. Based on our observations, PBM significantly increased optical density of OVX-BMMSCs (2.15 ± 0.11) compared to control -OVX-BMMSCs (1.55 ± 0.10) and healthy -BMMSCs (1.65 ± 0.10)(LSD test, both p < 0.05). Further, we found that both ALN, and ALN + PBM significantly increased optical densities of OVX-BMMSCs (24 h:2.40 ± 0.03;48 h:2.06 ± 0.00[ALN],both p < 0.01) and 1.88 ± 0.05[ALN + PBM], p < 0.05 compared to control -OVX-BMMSCs (24 h: 1.46 ± 0.01; 48 h: 1.83 ± 0.00 and 1.57 ± 0.08). It was concluded that PBM significantly increased cell viability of OVX-BMMSCs compared to control -OVX-BMMSCs and healthy -BMMSCs., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Postmenopausal osteoporosis (OP) is a major concern for public health. Low-level laser therapy (LLLT) has a positive effect on the health of bone marrow mesenchymal stem cells (BMMSCs). The purpose of this study is to evaluate the influence of LLLT and oxytocin (OT) incubation-individually and in combination-on osteoporotic BMMSCs in ovariectomized rats. Twelve female rats were randomized into two groups to undergo either a sham surgery (sham group) or ovariectomy-induced osteoporosis (OVX group). MSCs harvested from the BM of healthy and OVX rats underwent culture expansion. There were five groups. In Groups one (sham-BMMSC) and two (OVX-BMMSC) the cells were held in osteogenic condition medium without any intervention. In the group three (OT), OT incubation with optimum dose was performed for 48 h (two times, 10 -12 molar). In Group four, laser-treated-OVX-BMMSCs were treated with optimum protocol of LLLT (one time, 1.2 J/cm 2 ). In Group five (laser + OT group), the OT incubation plus the laser irradiation was performed. The biostimulatory effect of LLLT is demonstrated by a significant increase in the viability of OVX-BMMSCs, cell cycle, and extracellular levels of Transforming growth factor beta (TGF-β), insulin-like growth factor-I (IGF-I), and Alkaline phosphatase (ALP) compared to control OVX-BMMSCs and/or the sham group. OT incubation and laser + OT incubation have a positive effect on OVX-BMMSCs. However, LLLT is more effective statistically. We conclude that LLLT significantly improved cell viability, enhanced the osteogenic potential of the OVX-BMMSCs, and increased the extracellular levels of the TGF-β, IGF-I, and ALP., (© 2017 Wiley Periodicals, Inc.)