1. Functional properties of the human ventral mesencephalic neural stem cell line hVM1.
- Author
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Tønnesen J, Seiz EG, Ramos M, Lindvall O, Martinez-Serrano A, and Kokaia M
- Subjects
- Action Potentials physiology, Cell Culture Techniques, Cell Differentiation physiology, Cell Survival physiology, Culture Media pharmacology, Dopamine metabolism, Fetus cytology, Humans, Patch-Clamp Techniques, Tyrosine 3-Monooxygenase metabolism, Embryonic Stem Cells cytology, Mesencephalon cytology, Neurons cytology, Neurons physiology, Stem Cell Transplantation
- Abstract
The human fetal ventral mesencephalon-derived stem cell line, hVM1, yields high number of tyrosine hydroxylase-expressing presumed dopaminergic neurons upon in vitro differentiation. Here we report that cells generated from this line differentiate into a neuronal phenotype, express electrophysiological properties of functional neurons and respond to neurotransmitters in vitro. However, the electrophysiological properties are immature and the cells require longer maturation time than possible under in vitro conditions., (Copyright (c) 2009 Elsevier Inc. All rights reserved.)
- Published
- 2010
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