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Background: This work is development of new molecules of isoniazid derivatives as dealing with potential of antimicrobial activity against clinical pathogens causing infectious disease. Antimicrobial of novel Mannich base derivatives can be achieved via one-pot synthesis in green chemistry approach. This method offers efficient, mild reaction conditions and high yields. In this study, totally 12 compounds (1a-l) was prepared and screened for cytotoxic and antimicrobial activities., Materials and Methods: Newly synthesised compounds were conformed via FT- IR, 1 H, and 13 C NMR (Nuclear Magnetic Resonance), and mass spectra analysis. All compounds were checked antibacterial activity against gram-positive bacteria of Enterococcus faecalis, Staphylococcus aureus and gram-negative bacteria of Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. All compounds were checked against antifungal activity against Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Aspergillus niger, and Microsporum audouinii. All compounds were screened for cytotoxic activity against the MCF-7 (Michigan Cancer Foundation-7) cancer cell line., Result: The compound 1g was highly (MIC: 0.25 μg/mL) active against gram-negative bacterial of P. aeruginosa, whereas other compounds 1e and 1h were more active (MIC: 2 μg/mL) in K. pneumoniae and also 1g (MIC: 2 μg/mL) was more active in E. faecalis than standard ciprofloxacin. Antifungal screening, the compound 1b was highly active (MIC: 0.25 μg/mL) against C. albicance,1g (MIC: 2 μg/mL) and 1h (MIC: 4 μg/mL) was significant of active against A. fumigatus, and the compound 1c (MIC: 4 μg/mL) was extremely active in M. audouinii than clotrimazole. Compound 1g (GI 50 = 0.01 μM) exhibited high activity against the MCF-7 cell line, while 1b (GI 50 = 0.02 μM) was equipotent active compared with standard doxorubicin., Conclusion: A novel set of isoniazid derivatives (1a-l) and 1h were synthesized and screened for antimicrobial and cytotoxic activities. We found some highly active molecules, which are evidencing to be a potential treatment of bacterial and fungal infection candidates., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The objective of this article was to investigate the enhancing effect of menthone, menthol and pulegone on the transdermal absorption of drugs with different lipophilicity and probe their mechanisms of action at molecular level. Five model drugs, namely osthole, tetramethylpyrazine, ferulic acid, puerarin and geniposide, which were selected based on their lipophilicity denoted by logKo/w, were tested using in vitro permeation studies in which Franz diffusion cells and rat skin were employed. Infrared spectroscopy and molecular dynamic simulation were used to investigate the effect of these enhancers on the stratum corneum (SC) lipids, respectively. Three compounds could effectively promote the transdermal absorption of drugs with different lipophilicity, and the overall promoting capacities were in the following increasing order: pulegone < menthol < menthone. The penetration enhancement ratio was roughly in parabolic curve relationships with the drug lipophilicity after treatment with menthol or menthone, while the penetration enhancement effect of pulegone hardly changed with the alteration of the drug lipophilicity. The molecular mechanism studies suggested that menthone and menthol enhanced the skin permeability by disordering the ordered organization of SC lipids and extracted part of SC lipids, while pulegone appeared to promote drug transport across the skin only by extracting part of SC lipids.

Peppermint is one of the valuable plants in the pharmaceutical industry. The reduction of water in Iran is one of the most important production limitations. The use of organic materials such as humic acid and wood vinegar can be useful to help produce medicinal plants to reduce the effects of drought stress. This research was carried out in order to investigate the effect of humic acid and wood vinegar foliar spraying on plant traits and main compounds of peppermint essential oil under drought stress conditions. A filed experiment was conducted as a split plot based on a randomized complete block design with three replications in the research farm of the Faculty of Agriculture of the Tarbiat Modares University in 2019. The experimental treatments included low irrigation regime at three levels (irrigation up to the field capacity and respectively after draining 25 (no stress), 40 (moderate drought stress) and 55 (severe drought stress) percentage of available water in the root zone) as the main plots factor was foliar application at seven levels (humic acid (1, 2, and 3%), wood vinegar (5, 10, and 15%) and no foliar application (control)) as a sub plots. The results showed that the interaction of foliar application and irrigation regimes had a significant effect on plant height, total dry weight, total chlorophyll, flavonoid, total phenol, essential oil percentage and yield, and essential oil profile. Maximum total dry weight was produced with foliar spraying of 2 and 3% humic acid in normal irrigation conditions with 3204 and 3259 kg ha-1, respectively, and moderate and severe water deficit stress, respectively, caused a decrease of 15.34 and 27.42% of the total dry weight compared to the control. Water deficit stress harmed the yield of essential oil; so that with the increase of the stress intensity, its value decreased, and moderate and severe water deficit stress, a decrease of 9.38 and 40.2 percent of essential oil yield, respectively, was observed. Increasing the concentration of humic acid from zero to 3% increased the total chlorophyll by 10.4%. The flavonoids increased by 14.6, 22.5, and 33.1 percent with the use of 1, 2, and 3 percent humic acid, respectively, and this increase with the use of 5, 10, and 15 percent wood vinegar was 14.3, 34.7, and 37.1 percent, respectively. It was 34.34 and 37.15 percent. By increasing the concentration of humic acid from zero to 3%, the essential oil content increased by 60.6%. While increasing the concentration of wood vinegar from zero to 15%, its amount increased by 90.7%. The highest amount of menthol was obtained from the application of 10% wood vinegar in normal irrigation conditions at 61.04%, and it was placed in a statistical group with the treatment of 2% humic acid application in moderate water stress conditions (60.1%). In addition, the results showed that with the use of humic acid and wood vinegar, the amount of menthol increased by 21.88 and 17.84%, respectively, compared to not using them. According to the water limitation and the results of this research, it is recommended to use 3% humic acid in optimal irrigation conditions and moderate water deficit stress and use 15% wood vinegar in severe water deficit stress conditions. [ABSTRACT FROM AUTHOR]

Analyzing exhaled breath for volatile organic compounds (VOCs) using thermal desorption–gas chromatography–mass spectrometry (TD-GC-MS) offers a non-invasive diagnostic approach for various diseases. Despite its promise, the method faces challenges like sampling heterogeneity and high costs. Following the European Respiratory Society's advocacy for methodological standardization, we developed the SPIRITAS (Standardized Product for Inexpensive Respiratory InvesTigation: A breath Sampler), a low-cost, disposable breath sampler. This study evaluates the SPIRITAS's effectiveness in detecting targeted VOCs. We tested the SPIRITAS using the Peppermint Experiment, a standardized protocol that allows for comparison between different breath sampling and analytical practices by assessing the ability to detect five peppermint-specific VOCs after ingestion of a 200-milligram peppermint oil capsule. We included ten subjects and performed six breath samples per participant, including a baseline measurement taken before ingestion. We used the Wilcoxon signed-rank test to evaluate whether baseline values were significantly lower than the peak values of the targeted VOCs. Additionally, we conducted an experiment utilizing humidified medical-grade air to identify any VOCs attributable to the SPIRITAS setup itself. Results showed successful detection of four out of five targeted "peppermint-associated" VOCs: alpha-pinene (p ≤ 0.01), beta-pinene (p ≤ 0.01), menthone (p = 0.01), and menthol (p = 0.02), indicating significant differences between the baseline and peak values in the volunteers' breath. However, detection of eucalyptol was inconsistent. In addition, we identified 16 VOCs that were released by the SPIRITAS, one of which remains unidentified. Our findings underscore the SPIRITAS's potential for clinical applications, paving the way for broader biomarker research. The combination of ease of use, low cost, reduced risk of contamination, and standardization makes SPIRITAS very suitable for large-scale international studies. Furthermore, we have demonstrated the SPIRITAS's effectiveness in detecting specific VOCs and identified 16 compounds originating from the SPIRITAS, ensuring that these compounds would not be mis-qualified as potential biomarkers in future clinical studies. [ABSTRACT FROM AUTHOR]

Mentha arvensis L. var. 'kosi', occasionally recognized as corn mint or field mint, belongs to the Lamiaceae family and is widely grown in temperate climates throughout Europe, western and central Asia, as well as North America for its medicinal significance (Aflatuni et al, 2005). The essential oil extracted from the mint plant reveals a rich assortment of secondary metabolites. Among these compounds are menthol, menthone, menthyl acetate, neomenthol, á-pinene, â-pinene, piperitone, limonene, tannins, and flavonoids. The recognition and analysis of these constituents contribute to a thorough understanding of the intricate chemical composition inherent in mint plant essential oil (Akram et al, 2011). The plant has long been utilized in Indian medicine to alleviate stomach problems, fevers, and headaches. The influence of phenolic compounds on antimicrobial, antibacterial and antioxidant activities, germination and early development of herbaceous woodland plants, and seed germination has sparked considerable attention. Menthol, obtained from the essential oil of the mint plant, is credited with therapeutic properties that effectively combat skin allergies. Additionally, it demonstrates diuretic properties and serves as an antiseptic and carminative agent. The authors observed that the stimulating effects of salicylic acid, methyl jasmonate and similar phenolic compounds can be used to increase the content present in the essential oil and medicinal and pharmacological values of the plants of the Lamiaceae family. This idea led us to try the other phenolic compounds' foliar application to various species of mint with an expectation to increase the quality of these plants. The present study had the goal to evaluate if the exogenous foliar application of different phenolic compounds (phenol, catechol, pyrogallol, picric acid and salicylic acid) at concentration of 1.0 mM can optimize the physiological, biochemical activities and essential oil production in 120-day-old mint plants. Growth and physiological, biochemical, and total essential oil content assessments inclusive of menthol, menthone, menthyl acetate indicated that 1.0 mM concentration of pyrogallol and salicylic acid were most potent at eliciting a general stimulation of mint metabolism. Overall, the findings demonstrate that salicylic acid and pyrogallol stimulate mint physiology and open up new avenues for biotechnological application on additional plant species with agronomic promise. [ABSTRACT FROM AUTHOR]

Introduction Mint has been used both as a medicinal and aromatic plant since ancient times. Peppermint volatile oil is one of the most important essential oils and has strong antimicrobial activity. The stage at which the maximum essential oil is obtained from the plant is not necessarily the stage at which the plant has the most biomass, therefore, the appropriate time for harvesting the plant should be determined according to the maximum amount of the effective substance and the yield of dry matter. In most of the methods of producing medicinal plants, quick access to the equipment for extracting effective substances is not possible, especially at high levels, and this requires drying a large part of the plants and extracting the effective substance in subsequent times. The quality of dried medicinal plants is defined by the content of their biologically active compounds. This study was aimed to determine the best time to harvest peppermint and the effect of different drying methods on the shoots biochemical compounds. Materials and MethodsIn order to increase the yield of peppermint medicinal plant essential oil, two separate experiments were designed and conducted in Shirvan Higher Education Complex (N 26 37', E 45 57', altitude 1067 m) in 2015. The aim of first experiment was finding the best harvest time at three levels (Before flowering, at flowering 50 and 100%) and the second experiment was finding the best method for shoot drying with nine levels (Microwave radiation at 90, 180, 360, 600 and 900 watts; Oven at 40 and 60°C; Shade; and Sun), were based on a completely randomized block design (CRBD) and a completely randomized design (CRD), respectively. In the spring of 2015, tillage operations were carried out and after preparing the land, 9 plots (2 × 3 meters) were created in it. The distance between the plots was considered 1.5 m. Rizhomes of peppermint plant was obtained from Mashhad Agricultural and Natural Resources Research Center and transplanted at 20 × 30 cm intervals on May 26. Some morpholocial and biochemical traits were measured. Essential oil extraction was done by distillation method with water and essential oil components were determined by GC/Mass method. Results and DiscussionThe effect of harvesting time was significant (p≤0.01) on the plant height, number of leaves per plant, wet and dry yield of peppermint plant. The height of the plant after 50% flowering stage did not show any significant difference, but the other investigated traits include the number of leaves per plant, the wet and dry yield of the plant, the yield of essential oil in terms of wet and dry weight, and the inhibition of free radicals in the branches in shoots harvested at the time of full flowering had the highest amount, which showed a statistically significant difference. The yield of essential oil in terms of dry matter in the full flower stage increased 1.8 times compared to the 50% flowering stage and 4.6 times compared to the pre-flowering stage. The percentage of essential oil components was also affected by the harvest time, and the highest amount was observed in the full flowering stage (98.92 percent), include the highest amount in menthol (42.39 percent) and menthone (19.13 percent). The use of micro rays caused the shortest drying time, so that with the increase of power of micro rays, the drying time decreased and the shoots were dried in 0.16 hours at the power of 900 watts. The oven application at 60 and 40 °C were statistically ranked second and third in terms of drying speed with 10 and 14.6 hours. Sun and shade drying needed 46 and 109.6 hours to reach constant moisture, respectively. The highest amount of essential oil was obtained in the shade drying method (2.13%) and the lowest amount was observed in the microwave application at 900 and 600 watt. (0.01%). After shade drying, the highest amount of essential oil was observed in the oven method at 40 °C (2%) and in the sun drying (1.44%). According to the results of this study, it is recommended to harvest the branches at the time of full flowering and dry them using oven at 40 °C to obtain more essential oil yield of peppermint shoots. ConclusionIn general, the harvesting time has a significant effect on the morphological characteristics and essential oil of the peppermint shoots. Harvesting peppermint in the full flowering stage resulted in the highest amount of essential oil and essential oil yield. The maximum height and number of leaves in the plant were obtained by harvesting at the time of full flowering, but the maximum wet and dry yield of shoots was observed at the harvest of 50% of flowering. The amount of menthol and menthone as the most important compounds of mint essential oil in the full flowering stage was higher than the other two harvesting times. Also, based on the results of this experiment, different methods of drying peppermint plant have various effects on the essential oil amount of peppermint shoots. The shortest time until drying was related to the use of microwave, oven, sun and shade, respectively, and the highest yield of essential oil was obtained in shade, oven at 40 °C, microwave at 90 watt and sun, respectively.

• Melatonin and iron play crucial roles in the growth and production of secondary metabolites in peppermint. • Increasing the concentration of melatonin and Fe-CTs NPs led to a significant increase in biomass accumulation, total carbohydrates, and leaf area in exposed plants. • The highest levels of CAT and APX activities were observed in plants treated with 100 mg L −1 melatonin and 15 mg L −1 Fe-CTs NPs. • The highest value of menthol was obtained in plants treated with 7.5 mg L −1 Fe-CTs NPs. • The highest L-Menthone content was observed in plants treated with 200 mg L −1 melatonin and 15 mg L −1 Fe-CTs NPs. Melatonin and iron play important roles in the growth processes and production of secondary metabolites in medicinal plants. To evaluate the effects of melatonin and Chitosan-Coated Iron Oxide Nanoparticles (Fe-CTs NPs) on peppermint growth, essential oil content and constituents, total carbohydrates and antioxidant enzyme activities, a factorial experiment was conducted based on randomized complete block design (CRBD) with three replications (n = 3). Treatments consisted of different concentrations of melatonin (0, 100, and 200 mg L −1) and Fe-CTs NPs (0, 7.5, and 15 mg L −1). During the experiment, foliar applications of melatonin and Fe-CTs NPs were performed three times with 20 days' interval. Treatments with melatonin, and Fe-CTs NPs had significant effects on the studied traits. The results showed that with increasing melatonin and Fe-CTs NPs concentrations, fresh and dry weights, total carbohydrates, and leaf area index were significantly (P < 0.05) increased in treated plants. The highest CAT and APX activities were observed in plants treated with melatonin at 100 mg L −1 and Fe-CTs NPs at 15 mg L −1. Eucalyptol, Menthyl acetate, Thiophene, 2-ethyl-5-(2-methylpropyl), Mint furanone, and Viridiflorol were found as the major essential oil constituents in peppermint plants under experimental treatments. Moreover, analytical results showed that the highest amount of Menthol (38.48 %) was obtained in plants exposed to Fe-CTs NPs at 7.5 mg L −1, however, the highest L-Menthone (30.53 %) was observed in plants treated with melatonin at 200 mg L −1 and Fe-CTs NPs at 15 mg L −1. The findings provide insights into the regulatory mechanisms of melatonin and Fe-CTs NPs on peppermint growth, physiology and biosynthesis of volatile compounds. [ABSTRACT FROM AUTHOR]

(−)‐Menthol is one of the most popular aroma compounds worldwide. While in the past mostly extracted from mint plants, today (−)‐menthol synthesis from other raw materials is becoming more relevant. Common starting materials for menthol synthesis are m‐cresol, citral and myrcene, but also substrates like menthone, mono‐ and bicyclic terpenes and terpenoids have been used for this purpose in the past. As for many applications (−)‐menthol of high purity is required, asymmetric syntheses and enantiomeric resolution of obtained raw products are applied for menthol production. This review gives an overview on the most important synthetic menthol production processes of the companies Symrise, Takasago and BASF and relevant literature in the field of menthol synthesis with a focus on the last 20 years. [ABSTRACT FROM AUTHOR]

Background: Peppermint (Mentha x piperita L.) is one of the most important medicinal plants which used in food, pharmaceutical, perfumery, and flavoring industry. Objective: This study was planned to investigate the effects of foliar application of salicylic acid and yeast extract on production of valuable essential oil components in peppermint. Methods: A completely randomized design experiment with nine treatments consisting salicylic acid (40, 80, 160 and 320 mg/l), yeast extract (0.25, 0.75, 1 and 1.5 g/l) and distilled water (control) with three replications was carried out under greenhouse conditions. Results: In total, forty compounds were identified in the essential oils of the plant aerial parts. Menthone, menthol, piperitone, isopulegol and γ-terpinene were the major compounds of the oils studied. Menthone and menthol were 16.69 % and 14.39 % of the essential oils, respectively. Salicylic acid and yeast extract were increased menthone, neomenthol, piperitone, γ-terpinene and isomenthol acetate production 42, 60, 39, 59 and 34 % higher than control plants, respectively. Foliar application with 320 mg/l salicylic acid gave the best result in the enhancement of the major essential oil components of treated plants. The results of correlation between essential oil constituents showed that the neomenthol content had a significant positive correlation with menthone (r = 0.865**), γ-terpinene (r = 0.848**) and negative correlation with isopulegol (r = -0.886**). Conclusion: The quality of essential oil of M. piperita were influenced by the foliar application of salicylic acid and yeast extract at the appropriate concentrations. Elicitation by 320 mg/l salicylic acid was the optimum treatment for menthone, neomenthol, γ-terpinene and piperitone production.

The semi-arid highlands offer favorable temperature and radiation conditions for cultivating medicinal plants organically. This practice not only supports the sustainability of the ecosystems in these regions but also presents a valuable opportunity for the production of medicinal plants. Peppermint, a highly sought-after medicinal plant, presents a favorable choice for farmers residing in semi-arid regions due to its versatile applications across various industries. To assess the biomass and essential oil (EO) of peppermint, the utilization of diverse fertilizer sources across different cuttings has been examined. The experimental treatments consisted of four fertilizer sources including poultry manure (POM), sheep manure (SHM), cattle manure (CAM), and chemical fertilizers (CHF), and different cuttings, namely first cutting (CUT1), second cutting (CUT2) and third cutting (CUT3). Plants treated with POM and SHM gave the highest biomass in CUT1 and CUT2, respectively. The nutrient content (macro- and micro-) raised with the application of POM and SHM. The highest EO content was recorded in POM treatment and CUT2. Plants amended with POM had the greatest EO yield in three cuttings. The major EO constituents were menthol (35.2%-58.3%), menthone (3.1%–33.6%), menthyl acetate (1.1%-9.4%), iso -menthone (0.41%-8.8%), with the highest percentages of these compounds reached in treatments with organic manures in various cuttings. High-quality EOs (high menthol and low menthoufuran content) were obtained with organic manure treatments in three cuttings. Plants amended with POM, CHF and CHF showed the highest antioxidant capacity of EO in CUT1, CUT2 and CUT3, respectively. Overall, the usage of POM and SHM can be recommended for increasing biomass and improving the quality of peppermint EO during the growth season, while maintaining ecosystem sustainability by replacing chemical fertilizers in the semi-arid highlands. [Display omitted] • This is the first report to study peppermint essential oil in three cuttings • The semi-arid highlands offer favorable conditions for essential oil production • The amendment with poultry and sheep manure allowed the highest plant biomass • The use of poultry and sheep manure raised peppermint's essential oil yield • Percentages of the main volatile compounds increased by applying organic manures [ABSTRACT FROM AUTHOR]

Peppermint, Mentha piperita L. belonging to the Lamiaceae family, contains a valuable essential oil known for its high menthol and menthone content. During the vegetation seasons of 2016 and 2017, field studies were conducted in Turkey's Isparta climate by using randomized blocks to examine the yield and oil composition of the new peppermint cultivars. In these studies, Multimentha produced the highest herb yield even though the quality of its essential oil is relatively poor due to its low menthol content. Yield levels of other cultivars were similar to each other with the same statistical group. In the study, Citaro, Tokat CL-8, and Chocolate varieties had high menthol contents and were within the same chemical group. Varieties reacted differently to years and harvesting seasons, including summer and autumn. The menthol content of Citaro and Tokat CL-8 cultivars was higher in the fall harvest compared to the summer harvest. On the other hand, the menthol content of the Chocolate cultivar remained stable throughout the seasons. The study has revealed that the yields of the varieties with high menthol are low, and the oils obtained from all these varieties except Multimentha conform to the European pharmacopoeia. [ABSTRACT FROM AUTHOR]

Peppermint (Mentha x piperita L.) is not only used as a medicinal plant with therapeutic activity but also as a tea infusion, herb, spice or as a flavoring agent in other preparations. However, its adverse side effects on health have been described. The outcome of this review revealed that peppermint and its main constituents (pulegone, menthone, menthol and menthofuran) are moderately toxic. Peppermint and its menthol isomers possess no major innate mutagenic, genotoxic or embryotoxic properties. Peppermint essential oil interacts with cytochrome P450 isoenzymes in human liver microsomes. It is contraindicated in patients with bile duct obstruction and gall bladder inflammation. In patients with gastrointestinal reflux or hiatus hernia, its use should be exercised with caution because it may exacerbate the symptoms of gastrointestinal reflux. [ABSTRACT FROM AUTHOR]

In this research, the effects of 2 different planting times (autumn and spring) on the yield and quality of peppermint (Mentha × piperita L. var. Mitcham) were investigated under marginal land conditions. The test was conducted in Çukurova region located in the Eastern Mediterranean by using a randomized complete block design with three replications. Based on the results, the highest plant height (43 cm), fresh herb yield (12763 kg ha-1), dry herb yield (3407 kg ha-1), dry leaf yield (1976 kg ha-1) and essential oil yield (56.3 Lt ha-1) was determined in spring planting. The maximum ratio of essential oil was found in the first cuttings (3.18%). The yield values were higher in the first cuttings on year basis. With increasing the number of cuttings, a gradual decrease was observed in yield components. As a result of the gas chromatography analysis, oxygenated monoterpenes constituted the major chemical component group of M. × piperita essential oil (74.21-80.86%). The highest oxygenated monoterpenes were specified in the 1st cuttings of both planting seasons. Menthol (23.2-37.46 %) and menthone (22.82-31.56 %) were identified as the major components. Menthol percentage was higher in spring planting, and this rate has decreased every year compared to the previous one. As the number of cuttings increased, menthone rate also enhanced. On the other hand, as the rates of menthone increased, there was a decrease in menthol rates. a-Pinene (2.33-5.61%), limonene (0.63-4.13%), 1,8-cineole (5.22-9.98%), menthofuran (1.47-4.72%), isomenthone (1.14-5.95%), menthyl acetate (2.14-5.2%) and neomenthol (1.09-2.5%) were determined as other important components. Although the agronomic yield obtained from marginal land conditions was relatively lower, it was found superior in terms of quality and has adapted to standards values. As a result, the cultivation of the M. × piperita is economically viable in marginal lands without irrigation problems. [ABSTRACT FROM AUTHOR]

This study evaluated the influence of seasonality in essential oil (EO) chemical composition of Mentha x piperita L. and Mentha viridis L. grown in southeast of Brazil. Leaves were collected from November 2011 until August 2013, in the middle of the following seasons: Spring (November), Summer (February), Autumn (May) and Winter (August). EO extraction was carried out by hydrodistillation in Clevenger apparatus for 1 hour. Chemical composition of EO was analyzed by GC-FID and GC-MS. The EO content and its chemical composition were influenced by seasonality in both species. The highest EO content was obtained for M. x piperita during spring (4.26%) and for M. viridis during spring (3.30%) and summer (3.70%). Mentha x piperita increased menthol (16.31 to 41.26%), neomenthol (3.02 to 6.39%) and menthone (5.56 to 41.58%) contents during spring and summer, respectively. Whereas, M. viridis EO content did not show a quality response pattern in relation to seasonality. Therefore, harvest time for M. x piperita is recommended during spring, and for M. viridis is recommended during spring and summer. [ABSTRACT FROM AUTHOR]

• Mentha piperita are exploited as a natural source of bioactive phytochemicals • Several processes are available for extraction of plant phytopchemicals • Different elicitors can be used to increase secondary metabolites production • Peppermint components have new applications against diseases of different origins • M. piperita compounds have clinical, industrial , and agricultural applications Mentha piperita L. (peppermint) is one of the most popular and widely consumed species worldwide, and is used in the medical as well as in industrial and culinary fields. The importance of M. piperita is mainly due to its essential oils (EOs) and polyphenols. The present work reviews various novelty aspects of M. piperita phytochemicals, including their components, processes used to obtain EOs and extracts , agricultural strategies for the induction of EO production, shelf-life processes, and their application in phytoremediation, animal science, herbicides, pesticides, food industries, and therapeutic issues. The amount and components of M. piperita EOs (MpEOs) available vary widely depending upon the region, climatic and agronomic conditions, the extraction technique and drying procedure. In recent years, different methodologies have been evaluated to enhance EO production and other phytochemicals, such as inoculation with plant growth-promoting rhizobacteria, application of chemical elicitors, titanium dioxide nanoparticles, fly ash, and environmental factors. MpEOs and phenolic compounds possess high antimicrobial, antioxidant, antispasmodic, anthelmintic, and acetylcholinesterase inhibitory activities. Due to the wide range of bioactivity and antioxidant activity, this is an interesting topic of research due to the growing usage of natural antioxidants as additives in food and cosmetics. However, further studies should be directed towards MpEOs and extract production and formulation, to enable their wide scale application. [ABSTRACT FROM AUTHOR]

Background: Nitrogen is the most important nutrient requirement for plants. Nitrogen supplying affected the leaf area, carbon fixation, glandular trichomes formation, ATP and NADPH content which resulted to the terpenoids biosynthesis enhancement and essential oils accumulation. Objective: This study was aimed to evaluate changes of essential oil content and components by use different levels of Urea fertilizer and its relation with the antioxidant status of peppermint. Method: The two field experiments were conducted on randomized complete block design at 2013 and 2014. The treatments consisted of three levels of nitrogen fertilizer (urea) (0, 75, and 150 kg ha-1). The evaluated traits were included antioxidant enzymes, essential oil percentage and essential oil components. Results: The urea fertilizer had a significant effect (P≤0.01) on the soluble protein amount and antioxidant enzymes activity. Urea consumption in the both years increased the peppermint essential oil content. The interaction of nitrogen fertilizer and year had a significant effect on most of the essential oil components excepted to β-pinene, myrcene, Limonene, 1,8- Cineole, Z-β- Ocimene, and α- Terpineol. Conclusion: Using nitrogen fertilizer increased the peppermint essential oil content. Also, nitrogen deficiency reduced the soluble proteins and essential oil content and in contrast, it increased antioxidant enzymes activity and pulegone content. Therefore, consumption of 75 kg urea per hectare is recommended due to increasing essential oil content, decreasing pulegone rate, and no significant effect on menthol, menthone, and menthofuran contents.

The aims of the present study were to evaluate the effects of different ventilation systems and sucrose concentrations on the growth, photosynthetic pigment content, and chemical composition of Mentha arvensis L. and M. viridis L. Photon flux (PPF) and growth were also evaluated based on M. arvensis heterotrophic, photomixotrophic, and photoautotrophic culture systems. Nodal segment explants were cultured on medium with 0, 15, and 30 g L−1 sucrose and four alternative membrane systems (AMS) in culture medium, namely no membrane (NMS), one (AMS1), two (AMS2), and four (AMS4) membranes. Mentha arvensis cultivation on medium containing 15 g L−1 of sucrose and four membrane (AMS4) led to the highest dry weight accumulation. Mentha viridis reached the same result with medium containing 30 g L−1 sucrose and AMS2 and AMS4. Mentha arvensis produced higher chlorophyll a, b and total chlorophyll, as well as carotenoids when cultured on medium with 30 g L−1 of sucrose and AMS4 and M. viridis when cultured on medium with 15 g L−1 of sucrose and AMS4. The NMS without sucrose favored higher pulegone and AMS4 induced higher menthol, menthone, and limonene from M. arvensis cultured in vitro. Furthermore, AMS2 induced menthol production and AMS1 increased trans-sabinene, linalol, and limonene content in M. viridis. Mentha arvensis plantlets cultivated under photoautotrophic system with 130 μmol m−2 s−1 showed improved growth and the highest leaf area. Thus, the recommendation to increase upper biomass and plantlet quality in mint species lies on employing an alternative membrane system under higher PPF. [ABSTRACT FROM AUTHOR]

Mentha arvensis, commonly named menthol mint or Japanese mint, is an important volatile oil- bearing plant having high amounts of natural menthol. M. arvensis volatile oil (VO) and menthol have a considerable share in the global trade of medicinal plants for widespread use in the pharmaceutical, food, cosmetic, and fragrance industries. This study was conducted to investigate the response of M. arvensis var. piperascens Holmes to planting time (fall and spring) under marginal land conditions. Plants were propagated by three-year-old rhizomes. Obtained results showed that the effect of growing seasons was not significant on the agronomic traits and VO yield. However, the effects of six different impressions during the experiment were significant on the studied parameters. The highest plant height (45 cm), fresh biomass (16692 kg ha-1), dry biomass (4736 kg ha-1), leaf dry yield (2816 kg ha-1), and VO yield (158 L ha-1) were observed in the 1st cuttings. The yield components decreased with the plants aging. The lowest values of the mentioned parameters were in the sixth harvest. Moreover, the VO content varied from 4.6-6.1 %. Menthol (52.19-73.53 %) and menthone (3.42-18.89 %) were identified as the most major VO components of M. arvensis. While the menthol percent decreased in the last cuttings, menthone increased correspondingly. Based on the results of this study, it can be suggested that the cultivation of mint in marginal land has a desirable agronomic yield and acceptable chemical properties. [ABSTRACT FROM AUTHOR]

The genus Mentha has been known as culinary source of minty note. Besides, the essential oil imparts value to consumer products like confectionery, cosmetics, and pharmaceutical preparations. A simple and sensitive enantioselective gas chromatographic method is described for the quantitative analysis of terpenoid enantiomers in essential oils using different cyclodextrin coated stationary phases. Limonene, menthone, menthol, and menthyl acetate were identified as pure (-)-enantiomers whereas isomenthone, neomenthol, pulegone, and piperitone as pure (+)-enantiomers in menthol mint, and peppermint oils, respectively. The selectivity of each enantiomer was also demonstrated. A β-cyclodextrin phase with diethyl substitution provides baseline resolution for all target enantiomers with selectivity ranged from 1.004 to 1.050. Moreover, permethylated or diacetylated-β-cyclodextrin showed no selectivity for (+/-)-menthol enantiomers. The current study may facilitate the origin authentication studies of mint oils. Investigation of enantiomers involved in menthol biosynthesis revealed that only (-)-menthol is present in the essential oils of Mentha species. [ABSTRACT FROM AUTHOR]

Quantitative and qualitative composition of essential oils of peppermint breeds Lebedinaya Pesnya, Lubenchanka, Lidiya, Ukrainskaya Perechnaya, Mama, Chornolista was investigated before and after clonal microreproduction by the method of isolated tissues and bodies culture in vitro. Methods of essential oil steam distillation, capillary gas chromatography and statistical analysis were used in the research. It is established that increase in essential oil quantity was observed for peppermint breeds on which reproduction and improvement іn vіtro technology was applied. As a result of clonal microreproduction of peppermint plants in culture іn vіtro on nutrient medium Murasige and Skug, in which the growth regulators 0.75 mg/l of 6-benzylaminopurine, 0.1 mg/l of adenine, 0.05 mg/l of indolil-3-acetic acid and 0.5 mg/l of gibberellins acid were added and virocide Ribavirin in concentration 10 mg/l, improvement was obtained in comparison with vegetatively reproduced plants; increase in essential oil quantity per hectare was established for the following breeds; Chornolista by 54.2%, Lebedinaya pesnya by 38.2%, Ukrainskaya Perechnaya by 36.7%, Mama by 28.5%, Lubenchanka by 17.1% and Lidiya by 11.6%. For oil content the highest indices were noted for Lubenchanka, Mama and Lebedinaya Pesnya peppermint breeds with product yield 4.02%, 3.98% and 3.84% respectively. It was established that the essential oil component composition in non-clonal peppermint plants raw materials and plants-regenerants after culture in vitro is variable depending on breed. Limonene, cineole, menthone, menthofuran, iso-menthone, menthyl acetate, β-caryophyllene, iso-menthol, menthol, pulegone, germacren, piperitone, carvone were identified in peppermint essential oil. High content of menthol, low content of carvone, piperitone, pulegone (except for Chornolista, Ukrainskaya Perechnaya breeds) and menthofuran (except for Chornolista, Ukrainskaya Perechnaya and Lubenchanka breeds) are characteristic for Ukrainian selection peppermint investigated breeds. A clear tendency to menthol and menthone content ratio increase is observed in plants which were improved in conditions іn vіtro. Pulegone was not detected in essential oil samples of Lebedinaya Pesnya, Lidiya and Mama breeds. Biochemical markers of Lebedinaya Pesnya, Lubenchanka, Mama breeds, which differentiate them within the group of investigated breeds, are higher limonene, piperitone and menthol pool; for Ukrainskaya Perechnaya and Chornolista breeds – pulegone, cineole and menthone; for Lidiya breed – iso-menthone.

This work was aimed at quantitative and qualitative analyses of the essential oil of peppermint leaves under different drying methods. Thin layer drying experiments of the leaves were performed in shade, hot air dryer (at temperatures of 50°C, 60°C, and 70°C), and microwave oven (at power levels of 200, 400, and 800 W). Essential oils of the fresh and dried samples were extracted by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC/MS). The highest (22.24 g/kg dry matter) and the lowest (1.33 g/kg dry matter) oil yields were obtained from the hot air-dried leaves at temperature of 50°C and microwave-dried leaves at power of 800 W, respectively. In general, increasing drying temperature decreased the essential oil content. The GC/MS analysis of essential oils showed that the chemical compounds belonged mostly to oxygenated monoterpenes class (72.34–86.41%). The chemical compounds group was significantly (p

Peppermint is an important medicinal plant, and it is known for its essential oils and phenolic acids. Verticillium wilt is a vascular disease resulted from several Verticillium spp. causing significant economic losses in peppermint cultivation. In this study, the fungus Gibellulopsis nigrescens (syn. Verticillium nigrescens) was isolated from symptomless peppermint plants during the regular control of Verticillium wilt on peppermint in Germany. A pure fungal culture was prepared, and fungal DNA was extracted. Ribosomal internal transcribed spacer (ITS), beta-tubulin (TUB), and translation elongation factor 1-α (TEF1-α) were amplified, sequenced, and deposited in the GenBank. These sequences are located within the Gibellulopsis nigrescens cluster. Koch's postulate was fulfilled, and the fungus was re-isolated from the inoculated plants. Up to our knowledge, this is the first report of Gibellulopsis nigrescens on peppermint in Germany. [ABSTRACT FROM AUTHOR]

Mentha × Piperita L. or peppermint belonging to the family Lamiaceae (mint) is an important herb and useful in pharmaceutical, medicinal and cosmetic industries. In this study, the interactive effects of the foliar application of chitosan and inoculation of the rhizomes of peppermint seedlings with arbuscular mycorrhizal fungi (AMF) on growth and of the contents of menthol and balance of menthol/menthone of the essential oil from M × Piperita in a randomized complete block design (RCBD) were evaluated. The essential oil from the aerial parts (stem and leaves) of this herb was analyzed by GC-FID and GC/MS. Results indicated that the experimental treatments especially the inoculation of the peppermint plants with AMF influenced fresh and dry herbal weights and dry matter of the herb. The major constituents of the essential oil of M × Piperita were menthol and menthone belonging to oxygenated monoterpenes. The highest contents of essential oil yield, menthol and balance of menthol/menthone of the essential oil from M × Piperita were obtained from the peppermint plants under foliar spraying of chitosan and inoculation with AMF, however, the herbs under the foliar application of chitosan (both concentrations) without inoculation showed the highest percentages of menthone and limonene. The results indicated that elicitation of the peppermint with chitosan and inoculation of the peppermint plants with AMF has positive effects on dry herbal weight and secondary metabolites and can be improved the quantity and quality active substances of M × Piperita L. [ABSTRACT FROM AUTHOR]

In the current work, the biological potential of spearmint leaves essential oil (EO), including its antioxidant, antibacterial, and hemolytic properties, are described together with its chemical composition. The spearmint leaves were gathered in Punjab, Pakistan's hilly, dry, irrigated, and drought-stricken agroclimatic zones. From examined leaves from various locales, the hydro-distillation process produced an EO yield that ranged from 0.41% to 0.76%. In recovered EOs, GCMS compositional analysis identified 31 components, with carvone (27.4%–47.2%), menthone (1.0%–23.9%), menthol (3.0%–14.5%), limonene (2.4%–93%), and 1,8-cineol (0.6–8.5%) being the predominant constituents. The investigated oils displayed high levels of total phenolics (30.9–34.3 g GAE/100g), flavonoids (4.6–10.1 g CE/100g), and DPPH radical scavenging capacity (12.5–17.1 g mL−1) as well as linoleic acid peroxidation inhibition (57.1–62.6%). Against particular types of bacteria and fungus, the studied EO demonstrated considerable antibacterial activity (AMA). EO extracted from a sample of a location under drought stress (Layyah) shown greater AMA, while EO from hilly regions (Murree) displayed higher antioxidant potential, total phenolics (TP), flavonoids (TF), and hemolytic activity (HA). This study recommends the use of spearmint leaves as an effective natural antioxidant and antibacterial ingredient in the development of nutra-pharmaceutical products and dietary supplements. • Effect of agroclimatic parameters on the composition of Spearmint essential oil (MEO). • GC-MS profiling of tested essential oil. • Appraisal of antioxidant and antimicrobial potential. • Biological potential of tested essential oil was determined. [ABSTRACT FROM AUTHOR]

This research studied the chemical constituents and antibacterial activity of essential oils from the areal parts of Mentha × piperita L. essential oil. Essential oil was subjected to hydrodistillation for 4 h using Clevenger apparatus, resulting in nineteen chemical constituents representing 100% of the essential oil, comprising menthol (36.02%), menthone (24.56%), menthyl acetate (8.95%), and menthofuran (6.88%); these are major components, and others are minor components. Essential oil shows significant antibacterial and antifungal activity than principle components. The essential oil shows significant antibacterial activity against human pathogenic micro-organisms. Further, Staphylococcus aureus (42.44 ± 0.10 mm), Micrococcus flavus (40.01 ± 0.10 mm) , Bacillus subtilis (38.18 ± 0.11 mm), Staphylococcus epidermidis (35.14 ± 0.08 mm), and Salmonella enteritides (30.12 ± 0.12 mm) show the highest antibacterial activity against essential oils. Essential oils show significant antifungal activity against Alternaria alternaria (38.16 ± 0.10 mm), Fusarium tabacinum (35.24 ± 0.03 mm), Penicillum spp. (34.10 ± 0.02 mm), Fusarium oxyporum (33.44 ± 0.06 mm), and Aspergillus fumigates (30.08 ± 0.08 mm). The maximal and minimal inhibition concentration values are in the range of 10.22 ± 0.17 to 38.16 ± 0.10 and 0.50 ± 0.03 to 10.0 ± 0.14 μg/ml, for yeast and fungi respectively. The present study on essential oils deriving from the Mentha × piperita L. species could be used in antimicrobial activity as a natural source. [ABSTRACT FROM AUTHOR]

Abstract: The catalytic properties of the industrial "palladium on sibunit" catalyst (ICT-3-31, 0.5 wt % Pd) were studied in the reduction of menthone into menthol. Menthone was reduced both at 250 and 350°C using isopropanol as an H-donor under the conditions of the hydrogen transfer reaction (HTR). For comparison, a noncatalytic reaction was performed under the same conditions. At 350°C the conversion of menthone was the same (61-62%) in the presence of the catalyst and without it. In the reaction with the catalyst, the selectivity on the desired product menthol decreased considerably (from 98% down to 23%), and the amount of the by-products increased (from 2 up to 77% based on changed menthone). When the temperature of the catalytic reaction was lowered to 250°C, the reduction selectivity increased to 42%, but the conversion of menthone decreased to 10%. All the products of menthone conversion were identified and pathways of their formation suggested. At 250-350°C the carbon support sibunit catalyzed the dehydration of menthone, which hindered the satisfactory yields of the target alcohol. Other side reactions catalyzed by ICT-3-31 were dehydrogenation into p-menthenes and their further aromatization resulted in the formation of substituted benzenes—p-cymene and thymol. ICT-3-31 can be effectively used in HTR, if more reactive organic substrates are involved in the reaction below 200-250°C or stronger H-donors are applied. [ABSTRACT FROM AUTHOR]

Essential oil and major constituents of menthe were evaluated for fungicidal activities. Gas chromatography-mass spectrometry (GC-MS) of essential oil from leaves of Mentha arvensis cv. CIM-Saryu revealed that menthol was found in highest amount (77.94%) followed by isomenthone (5.24%), neomenthyl acetate (5.18%) and menthone (5.00%). Menthol and menthone were extracted from the essential oil by column chromatography. Essential oil, menthol and menthone were screened for their fungicidal activity against Rhizoctonia solani and Fusarium moniliforme. Menthol was highly effective as compared to essential oil as well as menthone. All of them exhibited less activity than standard bavistin at all the tested concentrations. [ABSTRACT FROM AUTHOR]

Influence of microwave power on dehydration kinetics as well as quantity, chemical composition and antioxidant potential of peppermint essential oil was evaluated. The leaves were dried using microwave powers of 200, 400 and 800 W, and the essential oils extracted by hydro-distillation. The antioxidant potential of the essential oils was quantified by DPPH radical-scavenging activity method. The obtained results showed that increasing microwave power caused significant decrement (p ≤ 0.01) in drying duration. Drying over the considered microwave powers reduced essential oil yield significantly (p ≤ 0.01). In total, 36 compounds were identified in the essential oils belonging mainly to oxygenated monoterpenes. The main constituents namely menthol, menthone and menthyl acetate showed significant variation (p ≤ 0.01 or p ≤ 0.05) with the drying process. Antioxidant assaying indicated significant effect (p ≤ 0.05) on radical scavenging activity of the essential oil by drying and essential oil concentration. Compared to the fresh samples (with an IC50 of 103 μg/ml), the leaves dried at microwave power levels of 200, 400 and 800 W resulted in significant lower antioxidant activities with IC50 values of 201, 291 and 615 μg/ml, respectively. [ABSTRACT FROM AUTHOR]

This work was aimed at quantitative and qualitative analyses of the essential oil of peppermint leaves under different drying methods. Thin layer drying experiments of the leaves were performed in shade, hot air dryer (at temperatures of 50°C, 60°C, and 70°C), and microwave oven (at power levels of 200, 400, and 800 W). Essential oils of the fresh and dried samples were extracted by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC/MS). The highest (22.24 g/kg dry matter) and the lowest (1.33 g/kg dry matter) oil yields were obtained from the hot air-dried leaves at temperature of 50°C and microwave-dried leaves at power of 800 W, respectively. In general, increasing drying temperature decreased the essential oil content. The GC/MS analysis of essential oils showed that the chemical compounds belonged mostly to oxygenated monoterpenes class (72.34-86.41%). The chemical compounds group was significantly (p < 0.01) decreased by microwave drying at power levels of 200 and 400 W. The assessed drying methods caused significant (p < 0.05 and/or p < 0.01) variations in the main constituents of the peppermint leaves essential oil including menthol, menthone, menthofuran, 1,8-cineole, and menthyl acetate. The minimum (35.01%) and maximum (47.50%) concentrations of menthol, as the major compound of the oil, were found in hot air-dried leaves at temperature of 50°C and microwave-dried leaves at power of 400 W, respectively. The percentage of menthone, as the second constituent in the essential oil, was significantly lost (p < 0.01) under microwave drying. [ABSTRACT FROM AUTHOR]

Mentha × piperita L. (peppermint) is one of the most economically important aromatic plants. The plant and its essential oil are used in medicine, cosmetics and food industry. Peppermint was obtained by crossing M. aquatica and M. spicata , and the industry is searching for new cultivars, with high biomass productivity and high content of essential oil (EO), with menthol and menthone as main components. A method of selection of clones and lines was used for breeding of the new cultivar ʻKristinkaʼ in East Slovakia. The present work describes the evaluation of the biomass production and qualitative and quantitative parameters of the essential oil during the breeding process of this cultivar. Average biomass production was 4.6 kg m 2 , average content of EO was 1.38%, with menthol and menthone amounts of 69.3 and 12.5%, respectively. [ABSTRACT FROM AUTHOR]

Cornmint (Mentha arvensisL.) constitutes most important source of therapeutic agents used in the alternative systems of medicine. The essential oil of cornmint has wide applications in pharmaceutical, agrochemical and flavoring industries worldwide. This study was conducted to explore the individual as well as combined effect of the best foliar concentrations of gamma-irradiated carrageenan (IC), triacontanol (TRIA) and 28-homobrassinolide (HBR) on growth, yield and quality of cornmint. Foliar application of IC, TRIA and HBR, applied individually on plants, significantly improved the plant attributes studied. However, combined application of these plant growth regulators (PGRs) was more effective compared to their individual application. In comparison to other applied treatments and the control, the combination of three PGRs (80 ppm IC + 10−6M TRIA + 10−7M HBR) proved to be the best for most of the growth, physiological, biochemical and agronomic parameters studied. Combined application of the tested PGRs excelled the control in per plant yield of menthol, menthone and menthyl acetate by 135.9 and 134.1%, 180.0 and 161.1% and by 225.0 and 187.5% at 100 and 120 days after planting, respectively. [ABSTRACT FROM AUTHOR]

The current study is carried out the effect of Nitrogen fertilization in levels (0, 46, 92, 138) kg/h and spraying of gibberllic acid at concentration (0, 50, 100) ppm and the Algamix at concentration (1,2) ml/L in percentage ratio for concentration of some active constituents for the oil of two species of Mentha (M. piperita and M. spicata). The result was showed, that the samples of oil was contained the following compounds: (Camphor, Menthone, Menthyl acetate, Menthol and Thymol), using the Gas-liquid Chromatography technique (GLC). It's illustrated that the summer harvest was exceeded to gave the highest concentration of menthone compounds about 11.47% without fertilization (N0) with spraying of algamix at concentration 1 ml/L of M. spicata, as well as thymol compound about 16.69% in fourth level of fertilization 138 kg/h with out spraying of M.spicata too. While, the autumn harvest was exceeded increasing percentage of camphor compound about 0.20% in the fourth level of fertilization 138 kg/h with spraying of algamix at concentration 1ml/L of M. piperita and Menthol compound which was presented 46.50% in the second level of fertilization 46 kg/h without spraying, and also Menthyl acetate at concentration 50.06% in the same level of fertilization of M. piperita with spraying of Algamix at concentration 1ml/L.

Background: In order to sustainable agriculture, applications of bio-fertilizers are as a gradual alternative to chemical fertilizers or at least to minimize the levels of these chemical. Objective: To determine the effects of some bio-fertilizers and urea fertilizers on agronomical and pharmaceutical yield of Peppermint. Methods: This study was done in a randomized complete block design (RCBD) with nine treatments and three replications. The treatments were control (no applying fertilizer), three different bio-fertilizers (4 & 8 kg.ha-1 for each one), and chemical urea fertilizer 75 & 100 kg.ha-1. Results: The result showed that fertilizer treatments had significant effects on plant height, stem fresh and dry weight of per ha, dry weight of leaf per ha, menthone content (p

The constituents of the volatile oil of air-dried aerial parts of Mentha pulegium L. (Lamiaceae) plants wildly growing in Northwest Iran were analysed by GC/MS. 46 components were identified, comprising 96.6% of the essential oil. Monoterpenes (78.9%) were the main class of the identified components followed by a minor proportion of sesqui-terpenes (11%). Oxygenated monoterpenes (75.3%) were the major subclass of volatile oil components with menthone (38.7%), menthol (11.3%), neomenthol (10.5%), and pulegone (6.8%) as major compounds. Sesquiterpene hydrocarbons (10.6%) were the highlighted subclass of 15 carbons sesquiterpenoidal compounds with (E)-caryophyllene (4.9%) and β-cubebene (2.5%) as their principle representatives. Furthermore, menthyl acetate (C12 acetylated monoterpene derived compound) was contained considerable amounts (5.2%) in the essential oil. In total, volatile oil composition of M. pulegium L. plants studied in the present experiment was characterised as a new menthone type with appreciable amounts of menthol and neomenthol, and it could be used as a potential source of these high value monoterpenes in pharmaceutical and food industries.