1. Presenilin 1 interaction in the brain with a novel member of the Armadillo family.
- Author
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Zhou J, Liyanage U, Medina M, Ho C, Simmons AD, Lovett M, and Kosik KS
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Caenorhabditis elegans genetics, DNA chemistry, Drosophila genetics, Membrane Proteins genetics, Molecular Sequence Data, Polymerase Chain Reaction, Precipitin Tests, Presenilin-1, Brain Chemistry genetics, Membrane Proteins metabolism
- Abstract
One approach to understanding the function of presenilin 1 (PS1), is to discover those proteins with which it interacts. Evidence for a function in developmental patterning came from C. elegans, in which a PS homologue was identified by screening for suppressors of a mutation in Notch/lin-12, a gene which specifies cell fate. However, this genetic experiment cannot determine which proteins directly interact with PS1. Therefore, we utilized the two hybrid system and confirmatory co-immunoprecipitations to identify a novel catenin, termed delta-catenin, which interacts with PS1 and is principally expressed in brain. The catenins are a gene family related to the Armadillo gene in Drosophila, some of which appear to have dual roles-they are components of cell-cell adherens junctions, and may serve as intermediates in the Wingless (Wg) signaling pathway, which, like Notch/lin-12, is also responsible for a variety of inductive signaling events. In the non-neuronal 293 cell line, PS1 interacted with beta-catenin, the family member with the greatest homology to Armadillo. Wg and Notch interactions are mediated by the Dishevelled gene, which may form a signaling complex with PS1 and Wg pathway intermediates to regulate the function of the Notch/lin-12 gene.
- Published
- 1997
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