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Start Over Author "Heuss C" Topic membrane proteins
3 results on '"Heuss C"'

1. Protein interactions implicated in neurotransmitter release.

Author

el Far O, O'Connor V, Dresbach T, Pellegrini L, DeBello W, Schweizer F, Augustine G, Heuss C, Schäfer T, Charlton MP, and Betz H

Subjects
Adenosine Triphosphatases metabolism, Amino Acid Sequence, Animals, Carrier Proteins metabolism, Conserved Sequence, Evolution, Molecular, Exocytosis, Models, Molecular, Models, Neurological, N-Ethylmaleimide-Sensitive Proteins, Nerve Tissue Proteins chemistry, Nerve Tissue Proteins metabolism, Nerve Tissue Proteins physiology, SNARE Proteins, Synaptosomal-Associated Protein 25, Membrane Proteins chemistry, Membrane Proteins physiology, Neurotransmitter Agents metabolism, Vesicular Transport Proteins
Abstract

Biochemical evidence indicates that the exocytotic release of neurotransmitters involves both evolutionary conserved membrane proteins, the SNAREs, as well as ubiquitous cytosolic fusion proteins, NSF and SNAPs. We have analyzed the biochemical properties and the physiological effects of these proteins. Our data suggest models how NSF, SNAPs and SNAREs may function in neurotransmitter exocytosis.

Published
1998
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2. Disruption of syntaxin-mediated protein interactions blocks neurotransmitter secretion.

Author

O'Connor V, Heuss C, De Bello WM, Dresbach T, Charlton MP, Hunt JH, Pellegrini LL, Hodel A, Burger MM, Betz H, Augustine GJ, and Schäfer T

Subjects
Amino Acid Sequence, Animals, Botulinum Toxins pharmacology, Cloning, Molecular, Membrane Fusion, Molecular Sequence Data, Nerve Tissue Proteins metabolism, Peptide Fragments metabolism, Presynaptic Terminals drug effects, Presynaptic Terminals ultrastructure, Protein Binding drug effects, Qa-SNARE Proteins, SNARE Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Synapses drug effects, Synapses ultrastructure, Synaptosomal-Associated Protein 25, Decapodiformes genetics, Membrane Proteins genetics, Membrane Proteins metabolism, Neurotransmitter Agents metabolism, Synapses metabolism, Vesicular Transport Proteins
Abstract

The membrane protein syntaxin participates in several protein-protein interactions that have been implicated in neurotransmitter release. To probe the physiological importance of these interactions, we microinjected into the squid giant presynaptic terminal botulinum toxin C1, which cleaves syntaxin, and the H3 domain of syntaxin, which mediates binding to other proteins. Both reagents inhibited synaptic transmission yet did not affect the number or distribution of synaptic vesicles at the presynaptic active zone. Recombinant H3 domain inhibited the interactions between syntaxin and SNAP-25 that underlie the formation of stable SNARE complexes in vitro. These data support the notion that syntaxin-mediated SNARE complexes are necessary for docked synaptic vesicles to fuse.

Published
1997
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3. The docking protein of chromaffin granules.

Author

Schaefer T, Hodel A, Heuss C, and Burger MM

Subjects
Animals, Cell Membrane metabolism, Exocytosis, Models, Biological, Neurons metabolism, Chromaffin Granules metabolism, Chromaffin System metabolism, Membrane Proteins metabolism, Neurotransmitter Agents metabolism
Published
1994
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