1. Folding DNA into a Lipid-Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins.
- Author
-
Dong Y, Chen S, Zhang S, Sodroski J, Yang Z, Liu D, and Mao Y
- Subjects
- Cryoelectron Microscopy, Glucosides chemistry, Hemolysin Proteins chemistry, Hemolysin Proteins metabolism, Membrane Proteins metabolism, Microscopy, Electron, Transmission, DNA chemistry, Lipids chemistry, Membrane Proteins chemistry, Nanostructures chemistry
- Abstract
Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared with the membrane-scaffolding-protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2018
- Full Text
- View/download PDF