1. Juniperus indica Bertol. extract synergized with cisplatin against melanoma cells via the suppression of AKT/mTOR and MAPK signaling and induction of cell apoptosis.
- Author
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Huang XF, Gao HW, Lee SC, Chang KF, Tang LT, and Tsai NM
- Subjects
- Animals, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Apoptosis drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Cisplatin therapeutic use, Dogs, Drug Synergism, G1 Phase Cell Cycle Checkpoints drug effects, Humans, MAP Kinase Signaling System drug effects, Madin Darby Canine Kidney Cells, Melanoma pathology, Mice, Plant Extracts therapeutic use, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction drug effects, TOR Serine-Threonine Kinases metabolism, Antineoplastic Combined Chemotherapy Protocols pharmacology, Cisplatin pharmacology, Juniperus chemistry, Melanoma drug therapy, Plant Extracts pharmacology, Skin Neoplasms drug therapy
- Abstract
Juniperus indica Bertol. is an herbal plant that belongs to the genus Juniperus , which is commonly used in traditional medicine to refresh the mind and for diuretic use. However, few studies have reported the function of J. indica Bertol. Hence, this study aimed to investigate the anti-tumor and synergistic potential of J. indica Bertol. extract (JIB extract) for melanoma cells. Our results indicated the anti-melanoma activity of JIB extract. JIB extract induced cell cycle arrest at the G
0 /G1 phase and decreased cyclin and cdk protein expressions. In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. According to the JIB extract's anti-melanoma capacity, to assess the synergistic effects of cisplatin and JIB extract. The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)- Published
- 2021
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