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Widiana A, Ukit, Thahirah A, Paijiah E. 2019. Diversity of endophytic fungi in cajuput leaf waste (Melaleuca cajuputi). Biodiversitas 20: 562-569. Cajuput Oil Factory at Jatimunggul, Indramayu of Forest Management Unit of Perum Perhutani (State Forest Enterprises) produces cajuput leaf waste during distillation process. This waste is difficult to decompose due to the presence of lignin in the secondary cell wall. Therefore, fungi are needed to produce some enzymes for speeding up the degrading process. This research was aimed at finding out the diversity of fungi growing in the cajuput (Melaleuca cajuputi Powell) leaf waste of zero, two and four months old. The result indicated that a total of 14 species of fungi belonging to four genera namely Aspergillus, Arthrinium, Rhizopus, and one unidentified genus are produced by cajuput leaf waste. Ten different types of endophytic fungi were isolated from two-month-old waste, seven Rhizopus and types of fungi were found in the zero-month-old waste, and only five types of isolates are found in the four-month-old waste. Three species of isolated fungi were also tested for their cellulase enzyme activity.

Waste of cajuput leaves (Melaleuca cajuputi Powell) has the potential to be used as an alternative cattle feed because it has a fairly high nutritional content. The nutritional content of cajuput leaf waste needs to be improved before being used as an alternative feed. The fermentation process can increase nutrient content in cajuput leaf waste and increase the digestibility of organic matter (OMD), dry matter digestibility (DMD), Ammonia (NH3), and Volatile Fatty Acid (VFA). This study aimed to determine the improvement of cajuput leaf waste nutrition with the help of yeast as well as the effect of fermented cajuput leaf waste along with a mixture of concentrates and field grass on digestibility, NH3, and VFA which were carried out in vitro test. The results showed the nutritional content of cajuput leaf waste fermented by yeast of Aspergillus chevalieri had the best value of protein 16.03%, fiber 16.92%, and fat 5.93%. The treatment R4 (50% cajuput leaf waste + 25% concentrate + 25% field grass) had the best digestibility (DMD=46.12%; DMO=32.08%), NH3 (8.37mM) and VFA (168.5mM) production.

Cajuput plant (Melaleuca cajuputi Powell) and its waste is one of the plants that can produce atsiri oils. Cajuput plant essential oil has the largest compound component, 1.8 cineol which has the ability as an antibacterial to kill pathogenic bacteria. The purpose of this study was to determine the antibacterial activity of cajuput leaf waste extract against pathogenic bacteria and find out the category of inhibition zones formed from extracts of cajuput leaf waste. This study used a completely randomized design method. Cajuput leaf waste used has a shelf life of 0, 2 and 4 months old. The pathogenic bacteria used consisted of 17 isolates, each treatment was repeated twice. The results showed that cajuput leaf extract had antibacterial activity known by the formation of inhibitory zones. The inhibitory zone in the extract of the 0-month leaf waste was greater than in the leaf extract of leaf of 2 months and 4 months. The diameter of the inhibition zone is at most 13 mm and at least 1 mm. With these results, it can be concluded that the antibacterial activity of cajuput leaf waste belongs to the category of low to strong.

4 Abstract: Distillation process of M. cajuputi Powell leaves to produce cajuput oil, yields a large amount of solid residue as waste materials. In vitro testing was conducted to evaluate the potential of solid residue of M. cajuputi Powell leaves as cattle feed. The measured variables were NH3, volatile fatty acids (VFA), Dry Matter Digestibility (DMD), and Organic Matter Digestibility (DMO). Completely randomized design was made with 5 different treatments and 4 replications, in order to get 20 experimental units. Data was analyzed by analysis of variance (ANOVA), followed by Duncan's multiple range test with a significance level of 5%. The results showed that giving 100% of the solid residue of M. cajuputi Powell leaves produced VFA, DMD and DMO for about 102.25 mM, 59.75% and 42.28% respectively, but with lesser amount of NH3 (2.54mM). These results were significant (P < 0.05) and are higher than the results by using field grass. It can be concluded that the solid residue of M. cajuputi Powell leaves has the potential to be used as cattle feed.

Gelam putih (M. cajuputi Powell) in Central Kalimantan, has not been exploited well. In vitro test conducted to evaluate the potential of gelam leaves as cattle feed. The measured variables were NH3, volatile fatty acids (VFA), dry matter Digestibility (DMD) and organic matter Digestibility (DMO). Completely randomized design was made with 5 different treatments and 4 replications, in order to get 20 experimental units. Data were analyzed by analysis of Variance (ANOVA) Followed by Duncan's multiple range test with a significance level of 5%. The results showed that the giving 100% of gelam leaves produced NH3 dan VFA for about 4.01 and 151.25 mM, also DMD and DMO by 58.35 and 52.38%. These results were significant (p