1. Extremely low frequency magnetic fields do not elicit oxidative stress in MCF10A cells
- Author
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Yun Sil Lee, Sung Ho Myung, Yoon Myung Gimm, Young Kyu Ko, Mi Na Hong, Sung Gil Chi, Na Kyung Han, Hyung Chul Lee, and Jae Seon Lee
- Subjects
medicine.medical_specialty ,Antioxidant ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Breast Neoplasms ,Intracellular reactive oxygen species ,Adenocarcinoma ,medicine.disease_cause ,Superoxide dismutase ,chemistry.chemical_compound ,Internal medicine ,Cell Line, Tumor ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Cell Shape ,Radiation ,biology ,Superoxide Dismutase ,Epithelial Cells ,Glutathione ,Equipment Design ,Enzyme assay ,Neoplasm Proteins ,Oxidative Stress ,Endocrinology ,Magnetic Fields ,chemistry ,Biochemistry ,Cell culture ,Gamma Rays ,Research Design ,biology.protein ,Female ,Reactive Oxygen Species ,Oxidation-Reduction ,Oxidative stress ,Intracellular - Abstract
The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.
- Published
- 2012