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The present study aimed to identify the relationship between glycemic variability (GV) and the 10-y risk of cardiovascular disease (CVD) in type 2 diabetes mellitus (T2DM) patients with good glycemic control.Two-hundred forty consecutive T2DM patients (HbA1c≤7.0%) without CVD were included to calculate the 10-y CVD risk by Framingham risk score (FRS), and divided into 3 groups: low-risk group (FRS≤10%), intermediate-risk group (10%, ≤20%) and high-risk group (20%). Inter-group differences of GV were determined by comparing the SD of blood glucose (SDBG), mean amplitudes of glycemic excursion (MAGE), and mean of daily differences (MODD) gathered from 72-h continuous glucose monitoring system.The levels of SDBG and MAGE significantly increased along with the raises of 10-y CVD risk of T2DM patients (p0.01). FRS was positively correlated with age, systolic blood pressure, SDBG and MAGE (r=0.717, 0.525, 0.509 and 0.485 respectively, p0.01), while negatively correlated with the level of HDL-C (r=-0.348, p0.01). Furthermore, multivariate logistic regression analysis confirmed that increased MAGE [OR: 1.623(1.198-2.316), p0.001] and patients with high urine albumin excretion rates [OR: 1.743(1.247-2.793), p0.001] were independent predictors for high 10-y CVD risk.GV predicts independently the 10-y CVD risk of T2DM patients with well-controlled HbA1c.

To investigate the effects of advanced glycation end products (AGEs) on the expression of angiopoietin-like protein 4 and its mechanisms in endothelials. Endothelial cells were incubated with various concentrations of AGEs for 24 hours, the expression of angiopoietin-like protein 4 were detected by

Objective To investigate the effect of Sini Decorction on preventing vascular restenosis after iliac artery balloon injury in rabbits, and the levels of serum cholesterol. Materials and methods Twenty four control group, model group and Sini Decorction group. Rabbits of control group were fed with common forage, but model group and Sini Decorction group were fed with high fat diet (1.0% cholesterol, 10% lard). Two weeks later, the iliac arteries of rabbits were injured by balloon for model group and Sini Decorction group, rabbits in control group were sham operated. After operation, rabbits in Sini Decorction group were intragastric administrated with Sini Decorction (Strobal: Dried Ginger: Radix Glycyrrhiza=5:3:2) 2 g/kg once a day. All rabbits were killed four weeks after operation and serum samples were stored to assay the levels of serum cholesterol, the microstructure of iliac arteries was observed by optical microscope, the ultrastructure of iliac arteries was investigated by transmission electron microscope and scanning electron microscope. Results Endotheliocytes lined up in order in iliac arteries of control group and Sini Decorction group, but the endotheliocytes desquamated in that of model group. Endothelium was completed and no foam cells were found in control group, but the endotheliocytes were desquamated and a great deal of foam cells were found in model group, endotheliocytes were normal and less foam cells were found in Sini Decorction group. The lumina was wider and intima was thinner in SND group than that of model group. The thickness of intima in model group exceeded that in control group and Sini Decorction group (1549.75±416.53 mm 2 vs 0.00 mm 2 vs 646.70±121.92 mm 2 , p 2 vs 526.43±155.85 mm 2 vs 334.55±129.63 mm 2 , p Conclusion Sini Decorction can lessen intimal hyperplasia and vascular stenosis, inhibit the development of artherosclerosis with iliac artery injury in rabbits, the mechanism may be connected with increasing the level of HDL-C, decreasing the serum levels of TC, TG and LDL-C.

Objective To investigate the relationship of urine albumin creatinine ratio (UACR), serum inflammatory factors and diabetic retinopathy. Methods One hundred and twenty five cases of type 2 diabetic patients age from 25 to 70 years old including 60 men and 65 women were recruited into this study. Another 47 normal glucose tolerance subjects were chosen as normal control group. They were divided into four groups after direct ophthalmoscope inspection and fundus fluorescein angiography (FFA) examinations as follows: 47 cases of healthy normal control (NC), 50 cases of type 2 diabetes patients without retinopathy (T2DM), 41 cases of diabetes with non-proliferative retinopathy (NPDR) and other 34 cases of diabetes with proliferative retinopathy (PDR). The physical examination was performed for each patient. UACR, serum levels of alanine aminotransferase (ALT), γ-glutamyl transpeptidase (GGT), fasting plasma glucose (FPG), lipid profiles, glycation haemoglobin A1c (GHbA1c), creatinine (Cr), uric acid (UA), fasting insulin (FINS) were measured. Insulin resistance index (HOMA-IR) was calculated. Intercellular adhesion molecule-1 (ICAM-1), tumour necrosis factor-α (TNFα) and adiponectin were also detected. Results There was no difference in UACR between T2DM without retinopathy patients and NC group, but ACR were higher in NPDR and PDR group than that of T2DM group (p Conclusion The results suggested that UACR, serum inflammatory factors such as ICAM-1, TNFα, adiponectin played important roles in the pathogenesis of diabetic retinopathy. UACR may be a clinical predictor and an important follow-up observation factor for DR.

Objectives To detect the effect of Sini Decoction on the expression of Sirt-1 and eNOS in EAhy926 cell injured by homocysteine. Methods Model of EAhy926 cell injured by homocysteine was made,the protection on the EAhy926 cell of Sini Decoction with different dosages were observed. NO concentration of cell culture fluid was detected by nitrate reductase method, effect of Sini Decoction on the expression of protein of Sirt-1 and eNOS in EAhy926 cell were observed by Western-blot, and effect of Sini Decoction on the expression of mRNA of Sirt-1 and eNOS in EAhy926 cell were observed by fluorescent quantitation PCR. Results After Model of EAhy926 cell injured by homocysteine was made, we found that cultured with 0.5,1.0,2.0,4.0,8.0 μmol/L homocysteine, cells grew less than cultured with normal culture medium, and with the increase of homocysteine concentration, the number of attached cell grew downwards obviously, as culturing with homocysteine 4.0 μmol/L for 24h did lower damage to cells and could induce effective cell injuring, it was made to be the model of injury. To detect the effect of Sini Decoction on EAhy926 cell injured by homocysteine, well growing EAhy926 cells were cultured in culture plate. 24 h later, cells were cultured with DMEM medium containing 2% fetal calf serum for 8 hours to make cells hungry, then cultured with medium containing Sini Decoction 0, 0.25, 0.5, 1.0g/ml respectively for 30 minutes,then cultured with medium containing homocysteine 4.0 μmol/L for 24 h. It was found that, compared with control group, attached cells in Sini Decoction groups grew better, and attached cells in Sini Decoction 1.0g/ml plus homocysteine 4.0 μmol/L group grew best. 4.0 μmol/L. Detected by nitrate reductase method, it was found that compared with control group, there was no obvious change of NO concentration of cell culture fluid in Sini Decoction 1.0 g/ml group, but in homocysteine 4.0 μmol/L medol group, NO concentration of cell culture fluid decreased oberviously, and in Sini Decoction groups, NO concentration of cell culture fluid increased, and in Sini Decoction 1.0g/ml plus homocysteine 4.0 μmol/L group it was the most obvious ( p p p Conclusions Homocysteine may injure EAhy926 cell by suppressing the expression of Sirt-1 then suppressing the expression of eNOS system, while Sini Decoction may protect EAhy926 cell by enhancing the expression of caveolin-1 then enhancing the expression of eNOS system.

Objectives To investigate the change of serum level of Pigment epithelium-derived factor (PEDF) in coronary heart disease patients. Methods 40 coronary heart disease patients, 29 males and 11 females were recruited into this study, which contains acute myocardial infarction 6 cases, unstable angina pectoris 15 cases, stable angina 19 cases, according to the New York Heart Association (NYHA) diagnostic criteria, ruling out infectious diseases, tumour, renal inadequacy, diabetes, Hepatic function damage, cardiac inadequacy, hyperthyrea, anaemia and hyperthermy. Control group contains 35 healthy people with similar gender, age and social background. Venous blood on an empty stomach were drawn next day after admission and were put in tubes, and were centrifuge in 1000 rpm for 10 min, then blood serum were imbibed and stored under −80°C. The serum of normal control group were taken in similar ways. Serum level of PEDF and IL-6 were determined by ELISA Kits (Bought from USCN life science Company, Wuhan, China and KeyGEN Biotech Company, Nanjing China). Meanwhile, serum level of total cholesterol, triglyeride and lipoprotein cholesterol were determined. Degree of stegnosis of coronary by Gensini scoring system based on the result of coronary arteriongraphy. Results The serum level of PEDF (8.39–2.64 ug/ ml) and Il-6 (7.40–4.41 pg/ml) in coronary heart disease group were higher than PEDF (6.952.92 µg/ml) and Il-6 (4.09–3.70 pg/ml) in control group (p 0.05), but there was correlativity between serum level of PEDF and IL-6 (r=0.241, p Conclusions The results suggest that Pigment epithelium-derived factor increases greatly in CHD patients, Pigment epithelium-derived factor may be an important factor of atherosclerosis.

Objectives To investigate the effects of Jiawei Buyang Huanwu Decoction on vascular stenosis and transforming growth factor -β1 (TGF-β1) after iliac artery were injured by balloon in diet-induced atherosclerotic rabbits. Methods 24 male New Zealand albino rabbits were equally randomized into control group, model group and drug group. The iliac arteries of the rabbits in the latter two groups were subjected to balloon injury. Four weeks later, serum TGF-β1 level was assayed, Endothelial hyperplasia, eNOS Protein and mRNA expression were observed in injured iliac artery. Results Optical microscope revealed narrowed vascular lumen, thicken intima and numerous arteriosclerotic plaques in the model group compared with the control group, whereas the vascular lumen and intima thickness remained basically normal in drug group. The serum TGF-β1 level was lower in drug group than that of model group, Immunohistochemistry and RT-PCR results showed that eNOS protein and mRNA expression was lower in rabbit iliac artery of drug group than that in model group. Conclusions Jiawei Buyang Huanwu Decoction can lessen intimal hyperplasia and vascular stenosis in iliac artery injury rabbits, and the mechanism of which may be related to decrease in TGF-β1 protein and gene expression.

Objectives To detect the effect of Sini Decoction on the expression of Caveolin-1 and eNOS in EAhy926 cell injured by homocysteine. Methods Model of EAhy926 cell injured by homocysteine was made, the protection on the EAhy926 cell of Sini Decoction with different dosages and different durations were observed. The effection of Sini Decoction on the expression of protein of Caveolin-1 and eNOS in EAhy926 cell were observed by Western-blot, and effection of Sini Decoction on the expression of mRNA of Caveolin-1 and eNOS in EAhy926 cell were observed by fluorescent quantitation PCR. Results After model of EAhy926 cell injured by homocysteine was made, we found that cultured with 0.5, 1.0, 2.0, 4.0, 8.0 µmol/l homocysteine, cells grew less than cultured with normal culture medium, and with the increase of homocysteine concentration, the number of attached cell grew downwards obviously, as culturing with homocysteine 4.0 µmol/l for 24 h did lower damage to cells and could induce effective cell injuring, it was made to be the model of injury. To detect the effection of Sini Decoction on EAhy926 cell injured by homocysteine, well growing EAhy926 cells were cultured in culture plate. 24 h later, cells were cultured with DMEM medium containing 2% fetal calf serum for 8 h to make cells hungry, then cultured with medium containing Sini Decoction 0, 0.25, 0.5, 1.0 g/ml respectively for 30 min, then cultured with medium containing homocysteine 4.0 µmol/l for 24 h. It was found that, compared with control group, attached cells in Sini Decoction groups grew better, and attached cells in Sini Decoction 1.0 g/ml plus homocysteine 4.0 µmol/l group grew best. Detected by western-blot, it was found that, compared with control group, there was no obvious change of protein of Caveolin-1 and eNOS in Sini Decoction 1.0 g/ml group, but in homocysteine 4.0 µmol/l medol group, expression of Caveolin-1 protein enhanced obviously, expression of eNOS protein weakened obviously, and in Sini Decoction groups, expression of Caveolin-1 protein weakened, and expression of eNOS protein enhanced, and in Sini Decoction 1.0 g/ml plus homocysteine 4.0 µmol/l group it was the most obvious p Conclusions Homocysteine may injure EAhy926 cell by enhancing the expression of caveolin-1 then suppressing the expression of eNOS, while Sini Decoction may protect EAhy926 cell by suppressing the expression of caveolin-1 then enhancing the expression of eNOS.

Objectives To investigate the effect of Jiawei Buyang Huanwu Decoction in preventing vascular restenosis and on the nitric oxide (NO) system after rabbit iliac artery balloon injury. Methods 24 male New Zealand albino rabbits were equally randomised into control group, model group and drug group. The iliac arteries of the rabbits in the latter two groups were subjected to balloon injury. Four weeks later, serum NO synthases (NOS) activity and endothelial nitric oxide synthase (eNOS) level was assayed, Endothelial hyperplasia, eNOS Protein and mRNA expression were observed in injured iliac artery. Results Optical microscope revealed narrowed vascular lumen, thicken intima and numerous arteriosclerotic plaques in the model group compared with the control group, whereas the vascular lumen and intima thickness remained basically normal in drug group. The serum NO level and total NOS activity were lower in model group than that of control group and drug group, while there is no difference between the latter two. Immunohistochemistry and RT-PCR results showed that eNOS protein and mRNA expression was stronger in rabbit iliac artery of control group and drug group than that in model group. Conclusions Jiawei BHD can lessen intimal hyperplasia and vascular stenosis in iliac artery injury rabbits, this effect is possibly related with that Jiawei BHD activated the NO system.

Objective To investigate the relationship of serum inflammatory factors, adiponectin and early atherosclerotic in type 2 diabetic patients (T2DM). Methods All 125 type 2 diabetic patients were divided into two groups according to the carotid intima-media thickness (CIMT) as follows: 65 cases of non-atherosclerotic diabetic patients with CIMT ≤0.9 mm and another 60 cases of atherosclerotic diabetic patients with CIMT > 0.9 mm or with plaque rapture being found. Thirty non-diabetic subjects were recruited as normal control. The clinical characteristics were collected for each patient. Serum fasting plasma glucose (FPG), glycation haemoglobin A1c (GHbA1c), fasting insulin (FINS), 2 h postprandic plasma glucose (2hPG), lipid profiles were measured. Serum levels of adiponectin, intercellular adhesion molecule−1 (ICAM-1), tumour necrosis factor-α (TNFα) and high sensitive C-reactive protein (hs-CRP) were also detected. Insulin resistance index (HOMA-IR) was calculated. Results (1) The serum levels of TNFα and hs-CRP, HOMA-IR were significantly higher in atherosclerotic patients than that of non-atherosclerotic and control subjects. However, the level of adiponectin in arteriosclerosis group was lower than other two groups. (2) There were negative correlation between adiponectin and CIMT, ICAM-1, TNFα, hs-CRP and HOMA-IR (r=0.574, −0.635, −0.681, −0.768, −0.752, p Conclusion The results suggested that inflammatory factors and adiponectin involved in the occurrence and development of diabetic vascular complications. The protective effects of adiponectin may exist in its anti-inflammation to improve insulin resistance and inhibit the development of atherosclerosis.

Objective To investigate the change of vascular endothelium in coronary heart disease patients by measuring the function of brachial artery endothelium with high definition power ultrasound. Method Thirty one coronary heart disease patients with heart failure, 21 males and 10 females were recruited into this study, According to the New York Heart Association (NYHA) classification, patients were divided into Class I (n=11), Class α (n=11), Class β (n=9), and all the patients were excluded from tumour and infection. Brachial artery endothelial function of all patients was measured next day after hospital admission. Normal control group consisted of 12 healthy people with age and gender matching. After measuring the inner diameter brachial artery with 7.0 MHz high definition power ultrasound linear array searching unit, reactive engorge test will be done. Distal end of target artery was pressed by pneumatic tourniquet up to 200 mm Hg (1 mm Hg=0.133 kPa), kept on 5 min, then deflated and decompressed. After 1 min later, inner diameter of same part of the brachial artery were measured. After when the inner diameter of brachial artery returned to fundamental numerous, patients took nitro glycerol 0.5 mg sublingually. In 2–5 min time, inner diameter of same part of the brachial artery were measured. Then percentage of changed numerous of inner diameter of brachial artery after pressing and taking nitro glycerol of fundamental numerous were calculated, named endothelium-depended vascular dilatation (Δ1%) and none endothelium-depended vascular dilatation (Δ2%) respectively. Result Endothelium-depended vascular dilatation (7.8–5.1%) and none endothelium-depended vascular dilatation (11.6–7.2%) in CHD patients decreased greatly than Normal control group (15.1–3.9%, 23.1–2.7%, p