34 results on '"Hanlin Zhang"'
Search Results
2. Inpatient Dermatology Consultations in a General Surgery Ward in a Tertiary Hospital in China: A Retrospective Study of 251 Patients
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Hanlin Zhang, Qiuning Sun, Hongzhong Jin, Rouyu Fang, and Keyun Tang
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medicine.medical_specialty ,Provisional diagnosis ,business.industry ,General surgery ,Retrospective cohort study ,Dermatology ,medicine.disease ,Inpatient dermatology consultation ,Drug eruption ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Plastic surgery ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Oral and maxillofacial surgery ,medicine ,Syphilis ,Medical diagnosis ,business ,Resident training ,Surgical incision ,Consultative dermatology ,Original Research - Abstract
Introduction Dermatologists play essential roles in providing dermatology consultations to inpatients admitted to hospital for care in another speciality ward. Data on dermatology consultations provided to inpatients admitted to general surgery wards are limited. The aim of this study was to analyze the reasons for and diagnoses of consultations provided by dermatologists to hospitalized patients in a general surgery ward and compare the provisional diagnoses by surgical residents and the final diagnoses by dermatologists. Methods Electronic health records were retrieved for patients admitted to a general surgery ward who received dermatology consultations while inpatients in Peking Union Medical College Hospital between 1 September 2015 and 31 August 2020. Sex, age, surgical diagnosis, reason for dermatology consultation, provisional diagnosis by surgical residents, and final diagnosis by dermatologists were reviewed. Results A total of 262 dermatology consultations for 251 patients (n = 251, 123 women and 128 men) were identified, of whom 240 (95.6%) required only one consultation and 11 (4.4%) required two. Dermatology consultations were classified into three categories: preoperative consultation (n = 45, 17.9%), postoperative consultation (n = 65, 25.9%), and consultation unrelated to general surgery diseases or treatments (including surgery) (n = 141, 56.2%). For consultations falling in the category preoperative consultation, common reasons for the consultation were: to evaluate whether the current treatment plan for previously diagnosed skin diseases needed to be changed; to evaluate and manage skin problems that emerged after admission; to evaluate syphilis; and to evaluate whether previously diagnosed skin diseases would affect surgical incision or wound healing. Drug eruption, dermatitis/eczema, infectious skin disorders, and urticaria were the most common skin diseases in the hospitalized general surgery patients. Only 32 (12.7%) provisional diagnoses were made, of which 25 (78.1%) were correct and seven (21.9%) were incorrect. Surgical residents mainly had difficulty distinguishing herpes zoster, drug eruption, and infectious skin disorders from dermatitis/eczema. Conclusion Our results facilitate the understanding of inpatient dermatology consultations in general surgery wards and may help in the design of future educational materials and/or management guidelines.
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- 2021
3. Use of Botulinum Toxin in Treating Rosacea: A Systematic Review
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Hanlin Zhang, Qiuning Sun, Yuanzhuo Wang, Keyun Tang, and Rouyu Fang
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medicine.medical_specialty ,Erythema ,Dermatology ,Cochrane Library ,law.invention ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,systematic review ,Randomized controlled trial ,law ,Medicine ,botulinum toxin ,Adverse effect ,Telangiectasia ,Original Research ,business.industry ,Retrospective cohort study ,medicine.disease ,Botulinum toxin ,rosacea ,Clinical, Cosmetic and Investigational Dermatology ,Rosacea ,030220 oncology & carcinogenesis ,medicine.symptom ,business ,medicine.drug - Abstract
Hanlin Zhang,* Keyun Tang,* Yuanzhuo Wang, Rouyu Fang, Qiuning Sun Department of Dermatology, Peking Union Medical College Hospital, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qiuning Sun Tel +86-10-69156114Fax +86-10-69156114Email doctorjenny1@163.comBackground: Rosacea is a chronic skin disorder characterized by erythema, flushing, telangiectasia, papules and pustules, phymatous changes, and ocular involvement. The aim of this study was to examine all published research articles in which botulinum toxin was used to treat rosacea and to evaluate the efficacy and safety of this treatment.Methods: PubMed, Embase, Cochrane Library, and Web of Science database were used to identify articles eligible for systematic review on March 26, 2021. Prospective or retrospective studies which directly used botulinum toxin to treat rosacea were included and reviewed.Results: Nine studies were included in this systematic review. Two were randomized controlled trials, graded as Level 2 for the quality of evidence. The total number of participants was 130, and the number of participants in each study ranged from 1 to 25. The improvement was observed in all studies in signs and symptoms compared with baseline. Adverse events were transitory and self-limited.Conclusion: Botulinum toxin could have overall satisfying efficacy and safety in the treatment of rosacea, though limited by small sample size, imperfect study design, and short follow-up visits.Keywords: botulinum toxin, rosacea, systematic review
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- 2021
4. miR17-92 cluster drives white adipose tissue browning
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Xiaomeng Liu, Rongcai Ye, Wanzhu Jin, Hanlin Zhang, Meng Dong, Lei Zhang, Yuanyuan Huang, Jun Lin, and Huiqiao Zhou
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Male ,0301 basic medicine ,medicine.medical_specialty ,Adipose Tissue, White ,Transgene ,Adipocytes, White ,Cell ,Adipose tissue ,Mice, Transgenic ,030209 endocrinology & metabolism ,White adipose tissue ,Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Adipose Tissue, Brown ,3T3-L1 Cells ,Internal medicine ,microRNA ,Brown adipose tissue ,Browning ,medicine ,Animals ,Humans ,Adipocytes, Beige ,Molecular Biology ,Cells, Cultured ,Mesenchymal stem cell ,Thermogenesis ,Mice, Inbred C57BL ,MicroRNAs ,Adipocytes, Brown ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,Multigene Family ,Cell Transdifferentiation - Abstract
White adipose tissue (WAT) browning may have beneficial effects for treating metabolic syndrome. miRNA are important regulators of the differentiation, development, and function of brown and beige adipocytes. Here, we found that the cold-inducible miRNA17-92 cluster is enriched in brown adipose tissue (BAT) compared with WAT. Overexpression of the miR17-92 cluster in C3H10T1/2 cells, a mouse mesenchymal stem cell line, enhanced the thermogenic capacity of adipocytes. Furthermore, we observed a significant reduction in adiposity in adipose tissue-specific miR17-92 cluster transgenic (TG) mice. This finding is partly explained by dramatic increases in white fat browning and energy expenditure. Interestingly, the miR17-92 cluster stimulated WAT browning without altering BAT activity in mice. In addition, when we removed the intrascapular BAT (iBAT), the TG mice could maintain their body temperature well under cold exposure. At the molecular level, we found that the miR17-92 cluster targets Rb1, a beige cell repressor in WAT. The present study reveals a critical role for the miR17-92 cluster in regulating WAT browning. These results may be helpful for better understanding the function of beige fat, which could compensate for the lack of BAT in humans, and may open new avenues for combatting metabolic syndrome.
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- 2020
5. Stigmatization in Patients With Psoriasis: A Mini Review
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Zihan Yang, Qiuning Sun, Keyun Tang, Hanlin Zhang, and Hongzhong Jin
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medicine.medical_specialty ,Coping (psychology) ,Mini Review ,media_common.quotation_subject ,Social Stigma ,Immunology ,Stigma (botany) ,Disease ,Quality of life (healthcare) ,stigmatization ,Surveys and Questionnaires ,Psoriasis ,medicine ,Humans ,Immunology and Allergy ,biologics ,Psychiatry ,media_common ,Stereotyping ,business.industry ,RC581-607 ,questionnaires ,medicine.disease ,Disfigurement ,Mental health ,Mental Health ,Feeling ,Quality of Life ,Immunologic diseases. Allergy ,business - Abstract
Psoriasis is a chronic and recurrent immune-related skin disease that often causes disfigurement and disability. Due to the visibility of lesions in patients and inadequate understanding of dermatology knowledge in the general public, patients with psoriasis often suffer from stigma in their daily lives, which has adverse effects on their mental health, quality of life, and therapeutic responses. This review summarized the frequently used questionnaires and scales to evaluate stigmatization in patients with psoriasis, and recent advances on this topic. Feelings of Stigmatization Questionnaire, Questionnaire on Experience with Skin Complaints, and 6-item Stigmatization Scale have been commonly used. The relationship between sociodemographic characteristics, disease-related variables, psychiatric disorders, quality of life, and stigmatization in patients with psoriasis has been thoroughly investigated with these questionnaires. Managing the stigmatization in patients with psoriasis needs cooperation among policymakers, dermatologists, psychologists, psychiatrists, researchers, and patients. Further studies can concentrate more on these existing topics, as well as other topics, including predictors of perceived stigmatization, stigmatization from non-patient groups, influence of biologics on stigmatization, and methods of coping with stigmatization.
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- 2021
6. Brown Adipose Tissue Activation by Cold Treatment Ameliorates Polycystic Ovary Syndrome in Rat
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Rongcai Ye, Chunlong Yan, Huiqiao Zhou, Yuanyuan Huang, Meng Dong, Hanlin Zhang, Xiaoxiao Jiang, Shouli Yuan, Li Chen, Rui Jiang, Ziyu Cheng, Kexin Zheng, Qiaoli Zhang, and Wanzhu Jin
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Ovulation ,medicine.medical_specialty ,endocrine system diseases ,Adipose Tissue, White ,Endocrinology, Diabetes and Metabolism ,Estrous Cycle ,Ovary ,Diseases of the endocrine glands. Clinical endocrinology ,Rats, Sprague-Dawley ,Endocrinology ,Adipose Tissue, Brown ,Ovarian Follicle ,cold treatment ,Corpus Luteum ,Internal medicine ,Brown adipose tissue ,medicine ,Animals ,Homeostasis ,Endocrine system ,Testosterone ,rat ,Original Research ,business.industry ,nutritional and metabolic diseases ,brown adipose tissue ,Dehydroepiandrosterone ,Luteinizing Hormone ,RC648-665 ,Polycystic ovary ,female genital diseases and pregnancy complications ,Rats ,Cold Temperature ,Transplantation ,Fertility ,medicine.anatomical_structure ,polycystic ovary syndrome ,Female ,ovary ,business ,Luteinizing hormone ,Infertility, Female ,Thermogenesis ,Corpus luteum - Abstract
Polycystic ovary syndrome (PCOS) is a common endocrine disease accompanied by energetic metabolic imbalance. Because the etiology of PCOS is complex and remains unclear, there is no effective and specific treatment for PCOS. It is often accompanied by various metabolic disorders such as obesity, insulin resistances, and others. Activated brown adipose tissue (BAT) consumes excess energy via thermogenesis, which has positive effects on energy metabolism. Our previous research and that of others indicates that BAT activity is decreased in PCOS patients, and exogenous BAT transplantation can improve PCOS rodents. Notably however, it is difficult to apply this therapeutic strategy in clinical practice. Therapeutic strategies of enhancing endogenous BAT activity and restoring whole-body endocrine homeostasis may be more meaningful for PCOS treatment. In the current study, the dehydroepiandrosterone-induced PCOS rat was exposed to low temperature for 20 days. The results show that cold treatment could reverse acyclicity of the estrous cycle and reduce circulating testosterone and luteinizing hormone in PCOS rats by activating endogenous BAT. It also significantly reduced the expression of steroidogenic enzymes as well as inflammatory factors in the ovaries of PCOS rats. Histological investigations revealed that cold treatment could significantly reduce ovary cystic follicles and increase corpus luteum, indicating that ovulation was recovered to a normal level. Concordant with these results, cold treatment also improved fertility in PCOS rats. Collectively, these findings suggest that cold treatment could be a novel therapeutic strategy for PCOS.
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- 2021
7. Oral GLP-1 Analogue Ameliorates Obesity-Induced Diabetes In Db/Db Mouse
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Hanlin Zhang, Meng Dong, and Wanzhu Jin
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medicine.medical_specialty ,Db/db Mouse ,Endocrinology ,business.industry ,Internal medicine ,Diabetes mellitus ,digestive, oral, and skin physiology ,medicine ,GLP-1 Analogue ,medicine.disease ,business ,Obesity - Abstract
Type 2 diabetes is currently experiencing an outbreak worldwide. GLP-1 effectively lowers blood glucose level as an emerging target for the treatment of type 2 diabetes. However, the application of GLP-1 is limited by short half-life and too expensive cost in clinic. In this study we employed the food-grade probiotics as delivery system to express human GLP-1 and its analogue. Recombinant lactococcus lactis could express GLP-1 and analogue in vitro and modified GLP-1 analogue was more resistant to DPP-4 degradation. Oral administration of GLP-1 analogue could reduce the fat mass. More importantly, GLP-1 analogue improved hyperglycemia and insulin resistance in Db/Db mouse although the insulin secretion is not observed in vitro. Our study demonstrates that lactococcus lactis genetically modified with single amino acid mutation could prolong half-life of GLP-1 and increase insulin sensitivity in Db/Db mouse model as an oral drug delivery system driving the development and innovation of drug therapy for type 2 diabetes.
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- 2021
8. Obeticholic acid ameliorates obesity and hepatic steatosis by activating brown fat
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Hanlin Zhang, Meng Dong, and Xiaomeng Liu
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obesity ,Cancer Research ,medicine.medical_specialty ,uncoupling protein 1 ,chemistry.chemical_compound ,obeticholic acid ,Primary biliary cirrhosis ,Immunology and Microbiology (miscellaneous) ,Internal medicine ,Brown adipose tissue ,Nonalcoholic fatty liver disease ,medicine ,Glucose homeostasis ,business.industry ,Obeticholic acid ,brown adipose tissue ,Articles ,General Medicine ,medicine.disease ,eye diseases ,Thermogenin ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Adipogenesis ,Steatosis ,business - Abstract
Obeticholic acid (OCA) is exemplified as a potent drug for treating primary biliary cirrhosis and nonalcoholic fatty liver disease by inhibiting bile acid synthesis. However, it remains unclear whether the effect of OCA is mediated by the function of brown adipose tissue (BAT). In the present study, brown adipogenesis differentiation in vitro and db/db mouse model treated with OCA were used to assess the anti-obesity function by body weight tracking, O2 consumption, food intake, physical activity, glucose tolerance tests. In addition, uncoupling protein 1 (Ucp1) protein expression in brown adipose tissue was measured by western blotting, morphometry of brown adipose tissue was analyzed by hematoxylin and eosin staining. Hepatic steatosis was detected by Oil-Red O staining and serological analysis was performed to assess the effect of OCA on hyperlipidemia. OCA treatment enhanced brown adipocyte cell differentiation and upregulated the expression of the BAT-specific gene Ucp1) in C3H10T1/2 cells in vitro. Consistent with these findings, OCA increased whole-body energy metabolism and glucose homeostasis by enhancing BAT activity in vivo, and ultimately decreased body weight gain in db/db mice. In addition, the results demonstrated that spontaneous hepatic steatosis in db/db mice was ameliorated following OCA treatment. In summary, OCA functioned as a BAT activator to help ameliorate obesity and maintain glucose homeostasis in db/db mice. The present results may provide a novel potential therapeutic approach to activate brown fat in patients with obesity and other metabolic disorders.
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- 2021
9. Topical calcineurin inhibitors as a double-edged sword in rosacea: A systematic review
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Yuchen Wang, Keyun Tang, Rouyu Fang, Qiuning Sun, Leyan Yang, Hanlin Zhang, and Dingyue Zhang
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medicine.medical_specialty ,business.industry ,Administration, Topical ,Calcineurin Inhibitors ,Dermatology ,Evidence-based medicine ,Cochrane Library ,Controlled studies ,Chronic inflammatory disease ,medicine.disease ,Tacrolimus ,Calcineurin ,Pimecrolimus ,Rosacea ,medicine ,Humans ,business ,medicine.drug ,Skin - Abstract
BACKGROUND Rosacea is a chronic inflammatory disease mainly with skin or ocular manifestations. Topical calcineurin inhibitors, pimecrolimus and tacrolimus, can be used to treat rosacea. However, they can also induce rosacea-like eruptions. AIMS This study systematically reviewed the double-edged sword effects of pimecrolimus and tacrolimus on rosacea. METHODS Four databases were retrieved to search for articles on the effects of pimecrolimus and tacrolimus on rosacea, including Cochrane Library, Embase, PubMed, and Web of Science. Only English articles were included in the systematic review. Relevant data were collected, and the levels of evidence were evaluated. RESULTS 28 articles published between 2001 and 2016 were included. 11 articles were about pimecrolimus as the treatment of rosacea, 4 articles were about the pimecrolimus-induced rosacea, 9 articles were about tacrolimus as the treatment of rosacea, and 4 articles were about tacrolimus-induced rosacea. Participants for each study ranged from 1 to 200. Several types of outcome measurements were used for these publications. CONCLUSIONS Both pimecrolimus and tacrolimus might have double-edged sword effects on rosacea. Pimecrolimus and tacrolimus could be effective for rosacea. However, both of them could also induce rosacea. Larger, randomized, controlled studies on pimecrolimus and tacrolimus as the treatment of rosacea and studies on the mechanisms of pimecrolimus and tacrolimus in treating or inducing rosacea are needed. This systematic review emphasized the double-edged sword role of topical calcineurin inhibitors in rosacea, which may pave the way for future research.
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- 2021
10. The Relationship Between Alzheimer's Disease and Skin Diseases: A Review
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Dingyue Zhang, Keyun Tang, Qiuning Sun, and Hanlin Zhang
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bullous pemphigoid ,medicine.medical_specialty ,skin disease ,Neurology ,Referral ,integumentary system ,business.industry ,hidradenitis suppurativa ,Dermatology ,Disease ,Review ,psoriasis ,medicine.disease ,Psoriasis ,medicine ,Dementia ,Hidradenitis suppurativa ,Bullous pemphigoid ,Skin cancer ,business ,Alzheimer’s disease ,dementia - Abstract
Alzheimer’s disease is the most common type of dementia placing a heavy burden on the healthcare system worldwide. Skin diseases are also one of the most common health problems. Several skin diseases are associated with Alzheimer’s disease through different mechanisms. This review summarizes the relationship between Alzheimer’s disease and several types of skin diseases, including bullous pemphigoid, hidradenitis suppurativa, psoriasis, skin cancer, and cutaneous amyloidosis, and provides suggestions based on these associations. Neurologists, dermatologists, and general practitioners should be aware of the relationship between Alzheimer’s disease and skin diseases. Dermatology/neurology consultation or referral is necessary when needed.
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- 2021
11. Botulinum toxin in treating Hailey-Hailey disease: A systematic review
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Rouyu Fang, Keyun Tang, Hanlin Zhang, Yuchen Wang, and Qiuning Sun
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medicine.medical_specialty ,Pemphigus, Benign Familial ,business.industry ,medicine.medical_treatment ,Complete remission ,Dermatology ,Disease ,Intertriginous ,Cochrane Library ,medicine.disease ,Botulinum toxin ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Pemphigus ,0302 clinical medicine ,Hailey–Hailey disease ,030220 oncology & carcinogenesis ,medicine ,Humans ,Botulinum Toxins, Type A ,business ,Adjuvant ,medicine.drug - Abstract
BACKGROUND Hailey-Hailey disease is a rare disorder characterized by recurrent painful blistering, erosions, maceration in the intertriginous regions. Botulinum toxin has been used in the treatment of Hailey-Hailey disease. AIMS This study aimed to examine all published articles on botulinum toxin in the treatment of Hailey-Hailey disease, and to evaluate its efficacy and safety. METHODS PubMed, Embase, Cochrane Library, and Web of Science were used to identify eligible articles on August 8, 2020. The searching strategy was "(Hailey Hailey or Hailey-Hailey or pemphigus) and botulinum." RESULTS Sixteen articles including 38 patients described the use of botulinum toxin in treating Hailey-Hailey disease. Only one case had no response, while the other patients all had partial or complete remission. No side effects were reported. Nine articles including 10 patients mainly described other treatment options, and the patients were only treated with botulinum toxin previously. Their responses to botulinum toxin were limited: one was mild improvement, one was partial response, and the other eight failed. CONCLUSION Botulinum toxin is not almighty, but a promising alternative option. We recommend botulinum toxin as an adjuvant or supplemental treatment modality for severe and recalcitrant Hailey-Hailey disease. Larger studies are warranted to confirm its efficacy, safety, long-term effects, and cost performance.
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- 2021
12. Immune Checkpoint Inhibitors in Advanced Acral Melanoma: A Systematic Review
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Hanlin Zhang, Jiarui Li, Yuanzhuo Wang, Qingyue Zheng, and Shu Zhang
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0301 basic medicine ,Oncology ,combination drug therapy ,medicine.medical_specialty ,Cancer Research ,Combination therapy ,medicine.medical_treatment ,Ipilimumab ,Pembrolizumab ,lcsh:RC254-282 ,programmed cell death 1 receptor ,03 medical and health sciences ,0302 clinical medicine ,systematic review ,Internal medicine ,melanoma ,Medicine ,ipilimumab ,radiotherapy ,business.industry ,Immunotherapy ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Radiation therapy ,Clinical trial ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cutaneous melanoma ,immunotherapy ,Nivolumab ,business ,medicine.drug - Abstract
IntroductionAcral melanoma (AM) has different biological characteristics from cutaneous melanoma. Although systemic therapeutic strategies for advanced AM resemble those for advanced cutaneous melanoma, the evidence of the clinical use of immune checkpoint inhibitors (ICIs) for AM is still inadequate. We aimed to systematically analyze the therapeutic effects and safety profile of ICI treatments in advanced AM.MethodsThis systematic review was conducted in line with a previously registered protocol. Three electronic databases, conference abstracts, clinical trial registers, and reference lists of included articles were searched for eligible studies. The primary outcomes were therapeutic effects, and the secondary outcomes were the safety profiles.ResultsThis systematic review included six studies investigating anti-CTLA-4 immunotherapy, 12 studies investigating anti-PD-1 immunotherapy, one study investigating the combination therapy of anti-CTLA-4 and anti-PD-1, and one study investigating anti-PD-1 immunotherapy in combination with radiotherapy. In most studies investigating ipilimumab, the anti-CTLA-4 antibody, the objective response rate ranged from 11.4 to 25%, the median progression-free survival ranged from 2.1 to 6.7 months, and the median overall survival was more than 7.16 months. For studies discussing anti-PD-1 immunotherapy with nivolumab, pembrolizumab, or JS001, the objective response rate ranged from 14 to 42.9%, the median progression-free survival ranged from 3.2 to 9.2 months, and the median overall survival was more than 14 months. The combination therapy of anti-CTLA-4 and anti-PD-1 immunotherapy showed better efficacy with an objective response rate of 42.9% than single-agent therapy. The retrospective study investigating the combination therapy of anti-PD-1 immunotherapy and radiation showed no overall response. Few outcomes regarding safety were reported in the included studies.ConclusionsICIs, especially anti-CTLA-4 monoclonal antibodies combined with anti-PD-1 antibodies, are effective systematic treatments in advanced AM. However, there remains a lack of high-level evidence to verify their efficacy and safety and support their clinical application.
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- 2020
13. Rosacea and its comorbidities: Should be emphasized but should not be overemphasized
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Qiuning Sun, Rouyu Fang, Keyun Tang, Hanlin Zhang, and Yuchen Wang
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medicine.medical_specialty ,business.industry ,Rosacea ,MEDLINE ,Medicine ,Humans ,Dermatology ,Comorbidity ,business ,medicine.disease - Published
- 2020
14. Rosacea Treatment: Review and Update
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Keyun Tang, Qiuning Sun, Rouyu Fang, Hanlin Zhang, and Yuchen Wang
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medicine.medical_specialty ,Erythema ,Dermatology ,Review ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Topical therapies ,Medicine ,In patient ,Telangiectasia ,skin and connective tissue diseases ,business.industry ,Inflammatory skin disease ,Oral therapies ,Treatment options ,medicine.disease ,Treatment review ,Systemic comorbidities ,Treatment ,Rosacea ,030220 oncology & carcinogenesis ,Itching ,medicine.symptom ,business - Abstract
Rosacea is a chronic and inflammatory skin disease characterized by flushing, nontransient erythema, papules/pustules, telangiectasia, and phymatous changes. Secondary manifestations, such as itching, burning, or stinging, are often observed in patients with rosacea. In 2017, a phenotype-based approach for diagnosis and classification was recommended. With the update of the diagnosis and classification of rosacea, treatment options for patients with rosacea have attracted the attention of dermatologists. Here, we summarize the latest advances in rosacea treatment, including skin care and cosmetic treatments, topical therapies, oral therapies, laser- and light-based therapies, injection therapies, treatments for specific types of rosacea, treatments for systemic comorbidities, and combination therapies. The impact of the phenotype-based approach on rosacea treatment and future directions are also discussed.
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- 2020
15. Botulinum toxin as a double-edged sword in alopecia: A systematic review
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Keyun Tang, Rouyu Fang, Qingyue Zheng, Yuanzhuo Wang, Hanlin Zhang, and Qiuning Sun
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medicine.medical_specialty ,Common disease ,Dermatology ,Cochrane Library ,Cosmetic dermatology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Double-Blind Method ,Medicine ,Humans ,Dosing ,Botulinum Toxins, Type A ,Adverse effect ,Randomized Controlled Trials as Topic ,business.industry ,Alopecia ,Evidence-based medicine ,Botulinum toxin ,Clinical trial ,Neuromuscular Agents ,030220 oncology & carcinogenesis ,Quality of Life ,business ,medicine.drug - Abstract
Background Alopecia is a common disease influencing the quality of life of the patients. Botulinum toxin has been widely employed in cosmetic dermatology and was applied in some clinical trials to treat alopecia. Aims Hence, We conducted a systematic review to examine the safety and efficacy of using botulinum toxin in treating alopecia. Methods Cochrane Library, Embase, PubMed, and Web of Science databases were used to search for articles. A PICOS question was formed and the author, year of publication, number of participants, study design, product, dilution and dosing, outcome measurement, findings, side effects, and follow-up were retrieved. The Oxford Centre for Evidence-Based Medicine 2011 Levels of Evidence was used as a qualitative indicator to evaluate the levels of evidence. Results Eleven articles published between 2005 and 2019 were included. 9 of which were about treatment effects and 2 were concerning adverse effects. The total number of the participants was 106, and the number for each article was ranged from 1 to 50. Varying type of outcome measurements was used for the publications. 8 articles were evaluated as Level 4 and 1 was evaluated as Level 5 during the 9 articles with treatment effects. Conclusions The results showed that botulinum toxin may function as a double-edged sword and have a low degree of efficacy and safety in treating alopecia. The study may be useful to remind the physicians of adverse effects in clinical practice and pave the way for further research. A larger, randomized, placebo-controlled study is warranted.
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- 2020
16. Resuming work gradually in the context of COVID ‐19: Experience from a tertiary dermatology department in China
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Hongzhong Jin, Hanlin Zhang, and Keyun Tang
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China ,2019-20 coronavirus outbreak ,medicine.medical_specialty ,Letter ,Coronavirus disease 2019 (COVID-19) ,Pneumonia, Viral ,coronavirus ,MEDLINE ,Context (language use) ,Comorbidity ,Dermatology ,Skin Diseases ,Betacoronavirus ,COVID‐19 ,Humans ,Medicine ,Letters ,Pandemics ,biology ,SARS-CoV-2 ,business.industry ,Dermatology department ,COVID-19 ,General Medicine ,biology.organism_classification ,work resumption ,dermatology clinics ,Work (electrical) ,Family medicine ,Coronavirus Infections ,business - Published
- 2020
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17. Cutaneous manifestations of the Coronavirus Disease 2019 ( <scp>COVID</scp> ‐19): A brief review
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Rouyu Fang, Qingyue Zheng, Keyun Tang, Qiuning Sun, Hanlin Zhang, and Yuanzhuo Wang
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skin ,medicine.medical_specialty ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Skin rashes ,Pneumonia, Viral ,coronavirus ,review ,Dermatology ,medicine.disease_cause ,Skin Diseases ,Short Papers ,Betacoronavirus ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,COVID‐19 ,medicine ,Humans ,Short Paper ,Pandemics ,Livedo reticularis ,Coronavirus ,Skin manifestations ,cutaneous manifestations ,SARS-CoV-2 ,business.industry ,COVID-19 ,General Medicine ,Prognosis ,Rash ,030220 oncology & carcinogenesis ,medicine.symptom ,Coronavirus Infections ,business ,Skin lesion - Abstract
COVID‐19, first appeared in December 2019 in Wuhan, China, has been spreading quickly throughout the world. We reviewed the evidence on cutaneous manifestations of COVID‐19 based on PubMed database. The searching strategy was (COVID* or coronavirus*) and (dermatol* or skin* or cutaneous*). The publication time was limited to 2019 onward. After independent review by two authors, 14 studies with 228 confirmed cases were included in the analysis. A total of 60 patients developed skin rashes, and the age ranged from 8 to 84. Exanthematous eruptions potentially related to COVID‐19 infection were highly variable and heterogeneous. Skin lesions mainly appeared erythematous, urticarial, and vesicular (chicken pox‐like or varicelliform). Petechiae rash, livedo reticularis, and reactivation of oral HSV‐1 were also observed in single cases. Newly reported eruptions like vascular lesions and peculiar (perniosis‐like) skin lesions caused concern among dermatologists. Exanthems were widely distributed and were primarily located on the trunk. Associated symptoms, latency time, treatment, and prognosis were also carefully summarized. This study reviewed the recently published COVID‐19 studies with skin manifestations, which may pave the way for further research.
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- 2020
18. Preliminary evaluation of the safety and efficacy of oral human antimicrobial peptide LL-37 in the treatment of patients of COVID-19, a small-scale, single-arm, exploratory safety study
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Xizhou Guan, Enqiang Qin, Tanshi Li, Hongyue Li, Hanlin Zhang, Wanzhu Jin, Meng Dong, Jianwei Jiao, Aihua Zheng, Gang Sun, Le Tian, Chunlong Yan, Shibo Feng, Tianshu Zeng, Cong Feng, Zhe Luan, Shufeng Wang, Congyong Li, Yuying Zhao, Xiaoxiao Jiang, Wei Chen, Jinsong Mu, Chen Li, Haotian Yu, Chaoyue Zhao, Yang Li, and Yiming Zhao
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Body surface area ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Nucleic acid test ,Antimicrobial ,Gastroenterology ,Genetically modified organism ,law.invention ,Probiotic ,Pharmacokinetics ,law ,Oral administration ,Internal medicine ,medicine ,business ,Adverse effect - Abstract
Background& AimsThe Coronavirus Disease 2019 (COVID-19) has become a global epidemic and has caused a lasting and huge loss of life security, economic development and social stability in more than 180 countries around the world. Unfortunately, there is still no specific treatment for COVID-19 till now, therefore, at this point, all potential therapies need to be critically considered. LL-37 is one of the best-studied human antimicrobial peptide (AMPs) that has a broad-spectrum activity against bacteria and viruses. The use of living, genetically modified organisms (GMOs) is an effective approach for delivery of therapeutic proteins. The aim of this study was to determine the safety and efficacy of the Lactococcus lactis which has been genetically modified to produce the therapeutic human antimicrobial peptide LL-37 (herein after referred to cas001) in the patients of COVID-19.MethodsFirstly we constructed genetically modified food-grade probiotic, Lactococcus lactis, with sequence of seven tandem repeats of mature human LL-37 under control of the nisin-inducible nisA promoter to produce the cas001. A total of 20 healthy SD rats, half male and half female (There were five male and five female in the control group, the same in treatment group) were used to observe the acute toxic reaction and death after daily administration of cas001 for three weeks, which helps to provide necessary reference basis for clinical dose selection, verificaition of toxic reaction and possible target organs. According to the estimated clinical dosage of 1 × 108CFU /kg/day, considering the conversion of body surface area, the dose for rats should be multiplied by 6.17 to 6 × 108 CFU/kg/day. We administrated 100 times higher dose at 6 × 1010 CFU/ /kg/day to rats. In order to investigate the pharmacokinetics of cas001, male SD rats (body weight 250-300g, 1 × 1010 /animal, n=3) were given oral administration of LL-37 bacteria powder. The concentration of LL-37 in the blood before and after gavage was detected by ELISA kit (Hycult biotechnology Cat# HK321).Human clinical study was approved by Ethics committee of Chinese PLA General Hospital (S2020-074-04) and a total of 11 patients with mild symptoms were enrolled in Wuhan hankou hospital and Huoshenshan hospital. They were enrolled voluntarily and all patients signed informed consent. Among them, there were 5 males and 6 females, aged 55 ± 12 (36-70) years old, and the duration from onset to medication enrollment was 35 ± 19 (5-68) days. 6 patients were nucleic acid positive and 5 patients were nucleic acid negative when they were enrolled. All patients received the oral drug cas001 treatment according to requirement(1 × 109 CFU/capsule, 3 capsules/time, three times a day for 3weeks), with an average follow-up time of 33 ± 15 days (see table 1 for the results).FindingsWestern blot analysis shows that reasonable amount of LL-37 were induced by different concentrations of nisin, which means we have successfully constructed cas001. In the pre-clinical safety evaluation test, after three weeks administration of cas001, no adverse effects were observed on the rat’s body weight, food and water intake, hematological or serum biochemical parameters. The results showed that the LD50 of cas001 was higher than that of the 100 times of the expected clinical dose of 6 × 1010 CFU/day. These results showed that cas001 could be safe in animal experiments. In addition, rat pharmacokinetics results showed that the serum concentration of LL-37 reached peak 2 hours after gavage of cas001 and returned to basal level 6 hours after gavage. During study period, the volunteers did not feel any discomfort while taking the cas001 capsules, and two hours after oral administration, the concentration of LL-37 were increased in healthy volunteers.cas001 shows definite effect in the improvement of gastrointestinal symptoms and is possible to have effects in improving the systemic symptoms and respiratory symptoms and may play a role in the improvement of results of nucleic acid test and lung CT test. 11 patients enrolled showed good compliance, tolerance, subjective feeling and actively interacted with the doctors. None of the patients had any adverse reactions.ConclusionsBased on above observations, we conclude here that as an oral anti-viral agent, cas001 displayed good safety profiles. It is very hard to reach conclusion of clinical outcomes related to the cas001, although changes of several symptoms indicate encouraging findings.
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- 2020
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19. The Incidence of Pregnancy-Associated Cushing's Disease and Its Relation to Pregnancy: A Retrospective Study
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Keyun Tang, Zhaolin Lu, Huijuan Zhu, Kang Chen, Renzhi Wang, Sun Xu, Ming Feng, Hanlin Zhang, and Lin Lu
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0301 basic medicine ,Adult ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,pituitary corticotroph adenoma ,030209 endocrinology & metabolism ,Abortion ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Pregnancy ,Medicine ,Childbirth ,Humans ,low birth weight ,Pituitary ACTH Hypersecretion ,Original Research ,Retrospective Studies ,Cushing's disease (CD) ,pregnancy outcome ,lcsh:RC648-665 ,business.industry ,Obstetrics ,Incidence (epidemiology) ,Incidence ,Retrospective cohort study ,Cushing's disease ,medicine.disease ,Pregnancy Complications ,Low birth weight ,030104 developmental biology ,Gestation ,Female ,medicine.symptom ,business ,peripartum - Abstract
Purpose: Cushing's disease (CD) is one of the most severe endocrine disorders and primarily affects women of reproductive age. The peripartum period has been observed to be a common time to develop CD. This study aims to retrospectively analyze the clinical characteristics of CD potentially associated with pregnancy and to evaluate relevant pregnancy outcomes. Methods: Patients who underwent surgery from January 2010 to May 2019 at Peking Union Medical College Hospital (PUMCH) with biochemically and pathologically confirmed CD were retrospectively analyzed. Pregnancy-associated CD was defined as CD onset during gestation or within 12 months after delivery or abortion. Data including demographics, biochemical tests, magnetic resonance imaging (MRI) findings, and disease outcomes were obtained from all patients by reviewing their medical records. Information regarding pregnancy was collected through a supplementary online questionnaire. Results: In a series of female patients (n = 70) of reproductive age with childbearing desire, 27.1% (n = 19) met the criteria for pregnancy-associated CD. The timing of diagnosis of pregnancy-associated CD was 2.7 ± 3.4 years after symptom onset, and the overall remission rate for these women was 89.5%. Three patients with pregnancy-associated CD developed hypertension during pregnancy, two of whom had new-onset hypertension at 16 weeks of gestation and one of whom had a complication of severe diabetes. The rates of spontaneous abortion and preterm birth among the women with pregnancy-associated CD were 26.3 and 28.6%, respectively. The proportions of all low-birth-weight (LBW) newborns (p = 0.002) and term LBW newborns (p = 0.033) were significantly higher in the pregnancy-associated CD group than in the non-pregnancy-associated CD group. Conclusions: In this study, a total of 27.1% of women of reproductive age with CD had pregnancy-associated CD, which might be induced by the hormonal milieu of pregnancy. An increased risk of having a LBW newborn was observed among mothers with pregnancy-associated CD. A high degree of clinical suspicion for CD may be warranted in the peripartum period. Patients with symptoms suspicious for CD throughout pregnancy and after childbirth, such as early-onset hypertension, severe hyperglycemia, and persistent weight gain, should be carefully diagnosed and closely monitored by clinicians.
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- 2020
20. Contributions of dermatologists to COVID‐19 research: a brief systematic review
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Rouyu Fang, Qiuning Sun, Keyun Tang, Hanlin Zhang, and Yuanzhuo Wang
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2019-20 coronavirus outbreak ,medicine.medical_specialty ,skin ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Pneumonia, Viral ,coronavirus ,Dermatology ,Skin Diseases ,Short Papers ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Betacoronavirus ,0302 clinical medicine ,systematic review ,COVID‐19 ,Pandemic ,Medicine ,Infection control ,Short Paper ,Humans ,Personal protective equipment ,Pandemics ,Personal Protective Equipment ,Infection Control ,business.industry ,SARS-CoV-2 ,COVID-19 ,General Medicine ,Disinfection ,030220 oncology & carcinogenesis ,Family medicine ,business ,Coronavirus Infections ,dermatologist ,Dermatologists - Abstract
Coronavirus Disease 2019 (COVID‐19) has been declared a pandemic. We conducted a systematic review to reveal the contribution of dermatologists in COVID‐19 research. 298 articles were included and classified into cutaneous manifestations of COVID‐19, operating experience against COVID‐19, mechanisms and treatment of COVID‐19, disinfection and personal protective equipment(PPE)‐related skin diseases and other topics. The value of these articles and their impact on clinical impact were discussed and we hope that dermatologists can have a better understanding of these areas from this study. This article is protected by copyright. All rights reserved.
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- 2020
21. What dermatologists could do to cope with the novel coronavirus (SARS-CoV-2): a dermatologist's perspective from China
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Keyun Tang, Rouyu Fang, Qiuning Sun, and Hanlin Zhang
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2019-20 coronavirus outbreak ,medicine.medical_specialty ,China ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Pneumonia, Viral ,Dermatology ,medicine.disease_cause ,Betacoronavirus ,Pandemic ,medicine ,Humans ,Pandemics ,Coronavirus ,biology ,business.industry ,SARS-CoV-2 ,COVID-19 ,biology.organism_classification ,Infectious Diseases ,Severe acute respiratory syndrome-related coronavirus ,Severe acute respiratory syndrome coronavirus ,business ,Coronavirus Infections ,Dermatologists - Published
- 2020
22. Comparison of Reference Genes for Transcriptional Studies in Postmortem Human Brain Tissue Under Different Conditions
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Qing Zhang, Hanlin Zhang, Xiaojing Qian, Chao Ma, Fan Liu, Qian Yang, Wenying Qiu, Kang Chen, Pan Liu, and Tianyi Sun
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0301 basic medicine ,medicine.medical_specialty ,Neurology ,Physiology ,business.industry ,General Neuroscience ,Pain medicine ,General Medicine ,Human physiology ,Human brain ,Biology ,Bioinformatics ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Text mining ,medicine.anatomical_structure ,Reference genes ,Anesthesiology ,medicine ,business ,Letter to the Editor ,030217 neurology & neurosurgery - Published
- 2018
23. The top 100 most cited articles in rosacea: a bibliometric analysis
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Hanlin Zhang, Qiuning Sun, Yuanzhuo Wang, Rouyu Fang, and Keyun Tang
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medicine.medical_specialty ,Bibliometric analysis ,MEDLINE ,Dermatology ,Ocular rosacea ,Bibliometrics ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Epidemiology ,Medicine ,Humans ,biology ,Helicobacter pylori ,business.industry ,Publications ,medicine.disease ,biology.organism_classification ,Europe ,Infectious Diseases ,Rosacea ,030220 oncology & carcinogenesis ,Citation ,business ,Demodex - Abstract
Background Many articles in rosacea have been published. Bibliometric analysis is helpful to determine the most influential studies in a specific field. Objective To identify the top 100 most cited articles in rosacea using the bibliometric analysis method. Methods We searched in the Web of Science database on 20 November 2019. Articles were listed in descending order by their total citations. The top 100 most cited articles in rosacea were identified and analysed. Results The top 100 most cited articles were published between 1971 and 2015. The largest number of articles was published in a single interval in 2011-2015. The average annual citations were constantly ascending, and the total citations were positively correlated with annual citations. The 100 articles were classified into different research focuses: treatment (35%), pathogenesis (27%), clinical features and diagnosis (14%), pathophysiology (6%), associated diseases (4%), epidemiology (3%) and others (11%). A total of 19 articles were randomized controlled trials (RCT), 14 focused on the association between rosacea and Demodex, and five focused on the association between rosacea and Helicobacter pylori. Twenty-five publications focused on a specific subtype of rosacea, mainly papulopustular and ocular rosacea. The 100 articles were published in 32 journals. A total of 79 different first corresponding authors were from 20 different countries, mostly in North America and Europe. Steinhoff. M from University of California published the most articles as the corresponding author. Conclusions This study identified the top 100 most cited articles in rosacea and analysed their bibliometric characteristics, which may pave the way for further research.
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- 2019
24. Hepatokine Pregnancy Zone Protein Governs the Diet‐Induced Thermogenesis Through Activating Brown Adipose Tissue
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Qiwei Shen, Meng Dong, Yi Tao, Hanlin Zhang, Yanfang Wang, Yuanyuan Huang, Li Chen, Zhaoyun Zhang, Xiangnan Wu, Shouli Yuan, Xiaoxiao Jiang, Jun Lin, Xiaomeng Liu, Rongcai Ye, Min He, Wanzhu Jin, Chunlong Yan, and Huiqiao Zhou
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Adult ,Male ,obesity ,medicine.medical_specialty ,Science ,General Chemical Engineering ,p38 mitogen-activated protein kinases ,General Physics and Astronomy ,Medicine (miscellaneous) ,Pregnancy Proteins ,Biology ,Diet induced thermogenesis ,Diet, High-Fat ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Mice ,Adipose Tissue, Brown ,Internal medicine ,hepatokine ,Intermittent fasting ,Brown adipose tissue ,medicine ,Animals ,Humans ,General Materials Science ,RNA, Small Interfering ,Endoplasmic Reticulum Chaperone BiP ,Research Articles ,Uncoupling Protein 1 ,Mice, Knockout ,intermittent fasting ,General Engineering ,Thermogenesis ,brown adipose tissue ,Glucose Tolerance Test ,Middle Aged ,Thermogenin ,Mice, Inbred C57BL ,PREGNANCY ZONE PROTEIN ,Endocrinology ,medicine.anatomical_structure ,Female ,RNA Interference ,Signal transduction ,Research Article ,Signal Transduction - Abstract
Intermittent fasting (IF), as a dietary intervention for weight loss, takes effects primarily through increasing energy expenditure. However, whether inter‐organ systems play a key role in IF remains unclear. Here, a novel hepatokine, pregnancy zone protein (PZP) is identified, which has significant induction during the refeeding stage of IF. Further, loss of function studies and protein therapeutic experiment in mice revealed that PZP promotes diet‐induced thermogenesis through activating brown adipose tissue (BAT). Mechanistically, circulating PZP can bind to cell surface glucose‐regulated protein of 78 kDa (GRP78) to promote uncoupling protein 1 (UCP1) expression via a p38 MAPK‐ATF2 signaling pathway in BAT. These studies illuminate a systemic regulation in which the IF promotes BAT thermogenesis through the endocrinal system and provide a novel potential target for treating obesity and related disorders., This paper reports a novel hepatokine, pregnancy zone protein (PZP), which is significant induction by refeeding. Loss of function studies and protein therapeutic experiment in mice reveal that PZP promotes diet‐induced thermogenesis through activating brown adipose tissue (BAT) via p38 MAPK‐ATF2‐UCP1 signaling pathway in BAT. These studies provide a novel potential target for treating obesity and related disorders.
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- 2021
25. The willingness and its influencing factors on patients to participate in patient safety spontaneous reports: A cross-sectional online study in China
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Kun Qiao, Xinran Qi, Keyun Tang, Xingming Li, Han Liu, Xuecheng Gao, Zhun Xiang, Qianying Jin, and Hanlin Zhang
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medicine.medical_specialty ,Descriptive statistics ,business.industry ,030503 health policy & services ,Health Policy ,Biomedical Engineering ,Odds ratio ,Logistic regression ,Confidence interval ,Test (assessment) ,03 medical and health sciences ,Patient safety ,0302 clinical medicine ,Family medicine ,Medicine ,030212 general & internal medicine ,Ordered logit ,Patient participation ,0305 other medical science ,business - Abstract
Objectives To understand the public's awareness of patient safety and willingness to participate, and to provide references for national medical institutions to carry out patient safety education and formulate patient safety management measures. This study aimed to investigate public recognition of patient safety and willingness to safety reporting and to provide references for national medical institutions to carry out patient safety education and formulate patient safety management measures with evidence-based information on construction, management, and patient-education of the reporting system of patient safety. Methods Information including demographic characteristics, recognition of patient safety, comments on the Patient for Patient Safety program, comments on the spontaneous reports, and willingness to participate in spontaneous reports were collected by questionnaire and were presented in descriptive analysis. The chi-square test was used to analyze the association between the subjects’ willingness to participate in the patient safety report and related factors. The impact of these associated factors on the willingness to participate in the patient safety report was evaluated and were analyzed using Ordinal Logistic Regression. Odds ratios (OR) and 95% confidence intervals (CI) were reported. Results A total of 27,493 people were surveyed validly in this study, of which 56.8% (95%CI: 56.21%-57.39%) of the respondents expressed their willingness to participate in the report, and 40.3% (95%CI: 39.72%-40.88%) expressed their willingness under certain conditions Participating, only 2.9% (95%CI: 2.62%-3.18%) are unwilling to participate. The results of multiple logistic regression found that factors that promote increased reporting willingness include gender (answer "male" OR=1.129, 95%CI: 1.068-1.191), and patient safety understanding (answer "very well" OR=1.590, 95%) CI: 1.439-1.756), patient participation in the evaluation of the effect of improving patient safety (think "large" OR=1.966, 95% CI: 1.790-2.162), evaluation of the effect of patient safety voluntary reports (think "the effect will be great" OR =6.166,95%CI:5.376-7.071, considered "moderate" OR=2.286, 95%CI: 2.000-2.614); factors that reduce willingness to report include age (answer "below 20" OR=0.717,95% CI: 0.592-0.868); occupation (answer "medical staff" OR=0.741, 95%CI: 0.696-0.788); patient safety status evaluation (answer "general" OR=0.668, 95%CI: 0.523-0.852). Conclusions This study found that the public's recognition of patient safety and attitude towards safety reporting had significant effects on their willingness to participate in spontaneous reports. Pertinent measures, such as education of the public and construction of the reporting system, are expected to promote patient safety in China.
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- 2021
26. Understanding and caring for lesbian, gay, bisexual, and transgender patients in dermatologic clinics: A dermatologist’s perspective from China
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Yuchen Wang, Rouyu Fang, Qiuning Sun, Keyun Tang, and Hanlin Zhang
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China ,medicine.medical_specialty ,Perspective (graphical) ,Dermatology ,Transgender Persons ,Sexual and Gender Minorities ,Family medicine ,Transgender ,Lesbian gay bisexual ,medicine ,Humans ,Female ,Psychology ,Dermatologists - Published
- 2020
27. Intractable mechanical hemolytic anemia complicating mitral valve surgery: a case series study
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Kang Chen, Hushan Ao, Kaicheng Song, Jin Wang, Hongyang Fan, Yuelun Zhang, Chunhua Yu, and Hanlin Zhang
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Adult ,Male ,Reoperation ,medicine.medical_specialty ,Gastrointestinal bleeding ,lcsh:Diseases of the circulatory (Cardiovascular) system ,Anemia, Hemolytic ,Time Factors ,Heart Valve Diseases ,Mitral valve surgery ,030204 cardiovascular system & hematology ,Gastroenterology ,Hemolysis ,Sepsis ,03 medical and health sciences ,Hemoglobins ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Mitral valve ,Mechanical hemolytic anemia ,medicine ,Perioperative management ,Humans ,Aged ,Bioprosthesis ,Heart Valve Prosthesis Implantation ,business.industry ,Septic shock ,Jaundice ,Middle Aged ,medicine.disease ,Cardiac surgery ,Aortic cross-clamp ,medicine.anatomical_structure ,Treatment Outcome ,030228 respiratory system ,lcsh:RC666-701 ,Beijing ,Heart Valve Prosthesis ,Mitral Valve ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers ,Mechanical hemolysis ,Research Article - Abstract
Background Intractable, mechanical hemolytic anemia (IMHA) is a rare catastrophic complication following mitral valve surgery. We analyzed patient characteristics and IMHA management by reoperations after mitral valve surgery. Methods We collected medical records from mitral valve patients requiring reoperation due to IMHA. Inclusion criteria: hemoglobin Results Data from 25 IMHA cases included 10 (40%) early onset (1.3 (0.3,3.0) months) and 15 (60%) late onset (120 (24,204) months) cases. Early IMHA etiologies included surgical defects (6, 60%), uncontrolled infection (3, 30%) and Bechet’s disease (1, 10%). Late IMHA etiologies included degeneration (13, 87%), new infection (1, 7%) and trauma (1, 7%). There were more mechanical valves (15, 88%) than bio-valves (2, 12%); the main valvular dysfunction was paravalvular leak (16, 64%). IMHA manifestations included jaundice (18, 72%), dark urine (21, 84%), heart failure (16, 64%), acute kidney injury (11, 44%), hepatomegaly (15, 60%), splenomegaly (15, 60%) and pancreatitis (1, 4%). Laboratory results showed decreased hemoglobin (70 ± 14 g/L) and increased bilirubin (72 ± 57 μmol/L), lactate dehydrogenase (2607 ± 2142 IU/L) and creatinine (136 ± 101 μmol/L) levels. Creatinine level negatively correlated with hemoglobin level (B = -3.33, S.E. B = 1.31, Exp(B) = 368.15, P = 0.018). Preoperative medications included iron supplements (20, 80%), erythropoietin (16, 64%) and beta-blocker (22, 88%). Two patients died of cardiac causes before reoperation. The other 23 underwent reoperation with long surgical times (aortic cross clamp 124 ± 50 min, cardiopulmonary bypass 182 ± 69 min) and blood transfusions (red blood cells 6 (6, 8) units, plasma 600 (400,800) ml, platelet 1(0,2) units). Postoperative complications included cardiac dysfunction (5, 22%), arrhythmia (10, 43%), sepsis (6, 26%), pulmonary infection (5, 22%), gastrointestinal bleeding (3, 13%), cerebral hemorrhage (2, 9%), chronic renal dysfunction (1, 4%) and surgical hemorrhage (1, 4%). Five (33%) patients died after reoperation from cardiac dysfunction (3, 60%), septic shock (1, 20%) and self-discharge (1, 20%). Conclusions IMHA induces severe multi-organ dysfunction, contributing to high mortality. Perioperative management should focus on etiological treatment, organ protection, and blood management.
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- 2019
28. Correlations Between Single Nucleotide Polymorphisms, Cognitive Dysfunction, and Postmortem Brain Pathology in Alzheimer’s Disease Among Han Chinese
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Qian Yang, Hanlin Zhang, Changlin Gong, Chao Ma, Yuanyuan Xu, Tianyi Sun, Qing Zhang, Pan Liu, Kang Chen, Xiaojing Qian, Wanying Zhang, and Wenying Qiu
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0301 basic medicine ,Oncology ,Adult ,Male ,medicine.medical_specialty ,Neurology ,Physiology ,Apolipoprotein E4 ,Single-nucleotide polymorphism ,Disease ,Polymorphism, Single Nucleotide ,Antiporters ,Pathogenesis ,03 medical and health sciences ,ADAM10 Protein ,0302 clinical medicine ,Asian People ,Alzheimer Disease ,Internal medicine ,Genotype ,medicine ,Dementia ,Humans ,Cognitive Dysfunction ,Genetic Predisposition to Disease ,Pathological ,Aged ,Aged, 80 and over ,business.industry ,General Neuroscience ,Brain ,Membrane Proteins ,General Medicine ,Human brain ,Middle Aged ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,Original Article ,Female ,Amyloid Precursor Protein Secretases ,business ,030217 neurology & neurosurgery - Abstract
In this study, the distribution of five Alzheimer’s disease (AD)-related single nucleotide polymorphisms (SNPs) in the Han population was examined in combination with the evaluation of clinical cognition and brain pathological analysis. The associations among SNPs, clinical daily cognitive states, and postmortem neuropathological changes were analyzed in 110 human brains from the Chinese Academy of Medical Sciences/Peking Union Medical College (CAMS/PUMC) Human Brain Bank. APOE ε4 (OR = 4.482, P = 0.004), the RS2305421 GG genotype (adjusted OR = 4.397, P = 0.015), and the RS10498633 GT genotype (adjusted OR = 2.375, P = 0.028) were associated with a higher score on the ABC (Aβ plaque score, Braak NFT stage, and CERAD neuritic plaque score) dementia scale. These results advance our understanding of the pathogenesis of AD, the relationship between pathological diagnosis and clinical diagnosis, and the SNPs in the Han population for future research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12264-019-00343-2) contains supplementary material, which is available to authorized users.
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- 2019
29. Rutin ameliorates obesity through brown fat activation
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Chengbi Cui, Weidong Huang, Xiao-Long Qi, Jun Lin, Jicheng Zhan, Yuanyuan Huang, Gang Wei, Xiaomeng Liu, Zengqiang Yuan, Rongcai Ye, Hyuek Jong Lee, Wen Xie, Chuanhai Zhang, Hanlin Zhang, Tao Hu, Meng Dong, Baiqiang Zhai, Yilin You, Qingsong Liu, Wanzhu Jin, Donghao Li, Shunai Liu, Jun Cheng, Xiaoxue Yuan, and Huiqiao Zhou
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0301 basic medicine ,medicine.medical_specialty ,Rutin ,Mice, Obese ,Diet, High-Fat ,Biochemistry ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adipose Tissue, Brown ,Sirtuin 1 ,Internal medicine ,Adipocyte ,Brown adipose tissue ,Genetics ,medicine ,Animals ,Humans ,Glucose homeostasis ,Obesity ,Receptor ,Molecular Biology ,Uncoupling Protein 1 ,Glucose tolerance test ,medicine.diagnostic_test ,Chemistry ,High Mobility Group Proteins ,Glucose Tolerance Test ,TFAM ,Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ,Thermogenin ,Cold Temperature ,DNA-Binding Proteins ,HEK293 Cells ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Energy Metabolism ,Body Temperature Regulation ,Biotechnology - Abstract
Increasing energy expenditure through activation of brown adipose tissue (BAT) is a critical approach to treating obesity and diabetes. In this study, rutin, a natural compound extracted from mulberry and a drug used as a capillary stabilizer clinically for many years without any side effects, regulated whole-body energy metabolism by enhancing BAT activity. Rutin treatment significantly reduced adiposity, increased energy expenditure, and improved glucose homeostasis in both genetically obese (Db/Db) and diet-induced obesity (DIO) mice. Rutin also induced brown-like adipocyte (beige) formation in subcutaneous adipose tissue in both obesity mouse models. Mechanistically, we found that rutin directly bound to and stabilized SIRT1, leading to hypoacetylation of peroxisome proliferator-activated receptor γ coactivator-1α protein, which stimulated Tfam transactivation and eventually augmented the number of mitochondria and UCP1 activity in BAT. These findings reveal that rutin is a novel small molecule that activates BAT and may provide a novel therapeutic approach to the treatment of metabolic disorders.-Yuan, X., Wei, G., You, Y., Huang, Y., Lee, H. J., Dong, M., Lin, J., Hu, T., Zhang, H., Zhang, C., Zhou, H., Ye, R., Qi, X., Zhai, B., Huang, W., Liu, S., Xie, W., Liu, Q., Liu, X., Cui, C., Li, D., Zhan, J., Cheng, J., Yuan, Z., Jin, W. Rutin ameliorates obesity through brown fat activation.
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- 2016
30. Analysis of Brain Donors' Demographic and Medical Characteristics to Facilitate the Construction of a Human Brain Bank in China
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Pan Liu, Changlin Gong, Chao Ma, Mengyao Wan, Yongmei Chen, Qian Yang, Qing Zhang, Di Zhang, Wenying Qiu, Hanlin Zhang, Xinyu Hong, Kang Chen, Xiaojing Qian, and Naili Wang
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0301 basic medicine ,Male ,medicine.medical_specialty ,China ,03 medical and health sciences ,Body donation ,0302 clinical medicine ,medicine ,Humans ,Aged ,Biological Specimen Banks ,Aged, 80 and over ,business.industry ,General Neuroscience ,Brain ,General Medicine ,Human brain ,Tissue Donors ,Psychiatry and Mental health ,Clinical Psychology ,medicine.anatomical_structure ,Family medicine ,Donation ,Tissue bank ,Brain bank ,Female ,030101 anatomy & morphology ,Geriatrics and Gerontology ,business ,030217 neurology & neurosurgery - Abstract
The Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS/PUMC) Human Brain Bank was established in December 2012 and had accomplished 197 brain donations by November 2017. The brain bank was based on a large-scale willed body donation program in CAMS/PUMC starting from 1999. Demographic and medical characteristic analysis of brain donors was conducted to facilitate the construction of the brain bank. The average postmortem delay of brain donors was 17.7 h and 77.7% of these donors died less than 15 km away from the brain bank. Donors were predominantly with higher-level education (p < 0.001) and at an older age when registration (p < 0.001) and donation (p < 0.001) occurred. Our results elucidated the characteristics of donors in the CAMS/PUMC Human Brain Bank, which may provide useful information to target potential donors and improve the quality and quantity of brain specimens. The current study may pave the way for the construction of a nationwide network of standardized human brain banks in China.
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- 2018
31. Brown adipose tissue activation by rutin ameliorates polycystic ovary syndrome in rat
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Hyuek Jong Lee, Qingsong Liu, Gang Wei, Wanzhu Jin, Jun Lin, Tao Hu, Wonchung Lim, Xiaoxue Yuan, Hanlin Zhang, Meng Dong, Chuanhai Zhang, Yuanyuan Huang, Huiqiao Zhou, and Rongcai Ye
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0301 basic medicine ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Rutin ,Clinical Biochemistry ,Biochemistry ,Rats, Sprague-Dawley ,0302 clinical medicine ,Adipose Tissue, Brown ,Brown adipose tissue ,Whole Body Imaging ,Aromatase ,Nutrition and Dietetics ,biology ,Anti-Inflammatory Agents, Non-Steroidal ,Thermogenesis ,Polycystic ovary ,female genital diseases and pregnancy complications ,medicine.anatomical_structure ,Thermography ,Enzyme Induction ,Female ,Infertility, Female ,Anovulation ,Polycystic Ovary Syndrome ,endocrine system ,medicine.medical_specialty ,Dehydroepiandrosterone ,030209 endocrinology & metabolism ,Congenital Abnormalities ,03 medical and health sciences ,Insulin resistance ,Internal medicine ,medicine ,Animals ,Molecular Biology ,Hyperandrogenism ,Ovary ,nutritional and metabolic diseases ,Luteinizing Hormone ,medicine.disease ,Phosphoproteins ,Transplantation ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,biology.protein ,Anti-Obesity Agents ,Insulin Resistance ,Biomarkers - Abstract
Polycystic ovary syndrome (PCOS) is a complex endocrinopathy that is characterized by anovulation, hyperandrogenism and polycystic ovary. However, there is a lack of effective treatment for PCOS at present because the pathologic cause of PCOS has not been elucidated. Although it has been known that brown adipose tissue transplantation ameliorates PCOS by activating endogenous BAT, BAT transplantation is not applicable in clinic. Therefore, BAT activation with natural compound could be an effective treatment strategy for PCOS patients. Here, we found that 3 weeks of rutin (a novel compound for BAT activation) treatment increased BAT activation, thereby it improved thermogenesis and systemic insulin sensitivity in dehydroepiandrosterone (DHEA)-induced PCOS rat. In addition, the expression levels of ovarian steroidogenic enzymes such as P450C17, aromatase, 3β-HSD, 17β-HSD and STAR were up-regulated in rutin-treated PCOS rat. Furthermore, acyclicity and the serum level of luteinizing hormone were normalized, and a large number of mature ovulated follicle with a reduction of cystic formation were observed in PCOS rat after rutin treatment. Finally, rutin treatment surprisingly improved fertility and birth defect in PCOS rat. Collectively, our results indicate that rutin treatment significantly improves systemic insulin resistance and ovarian malfunction in PCOS, and our findings in this study provide a novel therapeutic option for the treatment of PCOS by activating BAT with rutin.
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- 2017
32. Fluvastatin Sodium Ameliorates Obesity through Brown Fat Activation
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Kentaro Nagaoka, Wanzhu Jin, Rongcai Ye, Huiqiao Zhou, Li Chen, Xiaoxiao Jiang, Yuanyuan Huang, Na Yin, Meng Dong, Hanlin Zhang, Chuanhai Zhang, and Jun Lin
- Subjects
Male ,obesity ,medicine.medical_specialty ,Fluvastatin Sodium ,White adipose tissue ,Article ,activator ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,Mice ,Adipose Tissue, Brown ,Internal medicine ,Brown adipose tissue ,medicine ,Animals ,Humans ,Glucose homeostasis ,Physical and Theoretical Chemistry ,Fluvastatin ,lcsh:QH301-705.5 ,Molecular Biology ,Cells, Cultured ,Uncoupling Protein 1 ,Spectroscopy ,business.industry ,Organic Chemistry ,fluvastatin sodium ,brown adipose tissue ,General Medicine ,medicine.disease ,Thermogenin ,Computer Science Applications ,Mice, Inbred C57BL ,Endocrinology ,medicine.anatomical_structure ,lcsh:Biology (General) ,lcsh:QD1-999 ,Adipogenesis ,Anti-Obesity Agents ,Steatosis ,Energy Metabolism ,business ,Thermogenesis - Abstract
Brown adipose tissue (BAT), an organ that burns energy through uncoupling thermogenesis, is a promising therapeutic target for obesity. However, there are still no safe anti-obesity drugs that target BAT in the market. In the current study, we performed large scale screening of 636 compounds which were approved by Food and Drug Administration (FDA) to find drugs that could significantly increase uncoupling protein 1 (UCP1) mRNA expression by real-time PCR. Among those UCP1 activators, most of them were antibiotics or carcinogenic compounds. We paid particular attention to fluvastatin sodium (FS), because as an inhibitor of the cellular hydroxymethyl glutaryl coenzyme A (HMG-CoA) reductase, FS has already been approved for treatment of hypercholesteremia. We found that in the cellular levels, FS treatment significantly increased UCP1 expression and BAT activity in human brown adipocytes. Consistently, the expression of oxidative phosphorylation-related genes was significantly increased upon FS treatment without differences in adipogenic gene expression. Furthermore, FS treatment resisted to high-fat diet (HFD)-induced body weight gain by activating BAT in the mice model. In addition, administration of FS significantly increased energy expenditure, improved glucose homeostasis and ameliorated hepatic steatosis. Furthermore, we reveal that FS induced browning in subcutaneous white adipose tissue (sWAT) known to have a beneficial effect on energy metabolism. Taken together, our results clearly demonstrate that as an effective BAT activator, FS may have great potential for treatment of obesity and related metabolic disorders.
- Published
- 2019
33. Zic1 negatively regulates brown adipogenesis in C 3 H 10 T 1/2 cells
- Author
-
Yuanyuan Huang, Wanzhu Jin, Hanlin Zhang, and Hyuek Jong Lee
- Subjects
Zinc finger ,PRDM16 ,medicine.medical_specialty ,Multidisciplinary ,Myogenesis ,Neurogenesis ,Oxidative phosphorylation ,Biology ,ZIC1 ,Cell biology ,Endocrinology ,Adipogenesis ,Internal medicine ,medicine ,Beta oxidation - Abstract
Zinc finger in the cerebellum 1 (Zic1) is known to regulate neurogenesis and myogenesis in the developmental stage and widely used as one of the brown adipocyte-specific markers. In this study, we examined the effect of Zic1 on brown adipogenesis. Overexpression of Zic1 attenuated the lipid accumulation and the expressions of PPARγ2 and C/EBPα in C3H10T1/2 mesenchymal stem cells. The mRNA levels of BAT-specific thermogenic genes (PRDM16, PGC-1α and UCP1) and fatty acid oxidation regulatory genes (PPARα, CPT1α, CPT1β and COX7α1) were suppressed in Zic1-overexpressed cells. Moreover, overexpression of Zic1 reduced the mitochondrial oxidative phosphorylation (OXPHOS) regulatory proteins including ATP5α, UQCRC2, SDHB and NDUFB5. These results indicate a potential role of Zic1 in the regulation of brown adipogenesis via inhibiting adipogenesis, fatty acid oxidation and mitochondrial OXPHOS.
- Published
- 2015
34. Brown adipose tissue transplantation ameliorates polycystic ovary syndrome
- Author
-
Yuanyuan Huang, Meng Dong, Tao Hu, Huiqiao Zhou, Jun Lin, Chuanhai Zhang, Hanlin Zhang, Haibin Wang, Hyuek Jong Lee, Xiaoxue Yuan, Qingsong Liu, Wanzhu Jin, Gang Wei, Jun Zhao, Han Zhao, Rongcai Ye, and Zi-Jiang Chen
- Subjects
0301 basic medicine ,Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,endocrine system diseases ,Dehydroepiandrosterone ,030209 endocrinology & metabolism ,Estrous Cycle ,Anovulation ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Adipose Tissue, Brown ,Internal medicine ,Brown adipose tissue ,medicine ,Animals ,Humans ,Insulin ,Analysis of Variance ,Multidisciplinary ,Adiponectin ,business.industry ,Hyperandrogenism ,nutritional and metabolic diseases ,medicine.disease ,Polycystic ovary ,female genital diseases and pregnancy complications ,Transplantation ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Treatment Outcome ,Female ,Insulin Resistance ,business ,Energy Metabolism ,Infertility, Female ,Polycystic Ovary Syndrome - Abstract
Polycystic ovary syndrome (PCOS), which is characterized by anovulation, hyperandrogenism, and polycystic ovaries, is a complex endocrinopathy. Because the cause of PCOS at the molecular level is largely unknown, there is no cure or specific treatment for PCOS. Here, we show that transplantation of brown adipose tissue (BAT) reversed anovulation, hyperandrogenism, and polycystic ovaries in a dehydroepiandrosterone (DHEA)-induced PCOS rat. BAT transplantation into a PCOS rat significantly stabilized menstrual irregularity and improved systemic insulin sensitivity up to a normal level, which was not shown in a sham-operated or muscle-transplanted PCOS rat. Moreover, BAT transplantation, not sham operation or muscle transplantation, surprisingly improved fertility in PCOS rats. Interestingly, BAT transplantation activated endogenous BAT and thereby increased the circulating level of adiponectin, which plays a prominent role in whole-body energy metabolism and ovarian physiology. Consistent with BAT transplantation, administration of adiponectin protein dramatically rescued DHEA-induced PCOS phenotypes. These results highlight that endogenous BAT activity is closely related to the development of PCOS phenotypes and that BAT activation might be a promising therapeutic option for the treatment of PCOS.
- Published
- 2016
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