Objective: To investigate the correlation between fibular head height and the incidence and severity of varus knee osteoarthritis based on three-dimensional reconstruction of the knee joint.Methods: The data of knee joint imaging in our hospital from June 2018 to June 2020 were collected. The degree of varus deformity of the knee was assessed at the superior hip-knee-ankle angle of the X-rays. Three-dimensional reconstruction of patient computed tomography(CT)data was performed by mimics software. The fibular head height, joint line convergence angle (JLCA) and medial proximal tibial angle (MPTA) were measured in a three-dimensional model. The patients were divided according to the Kellgren-Lawrence grade: group A: grade 0, group B: grade I, group C: grade II, group D: grade III, and group E: grade IV. The differences in age, gender, height, weight, body mass index(BMI), fibular head height, and degree of varus deformity (JLCA, MPTA, and coxa-knee-ankle angle) were compared. Ordinal multivariate logistic regression was used to analyze the correlation between fibular head height and Kellgren-Lawrence grade.Pearson correlation was used to analyze the correlation between fibular head height and Kellgren-Lawrence grade.Results: 232 patients (232 knees) were finally included in the study, with Kellgren-Lawrence grades of 28 in group A, 31 in group B, 49 in group C, 53 in group D, and 71 in group E. The differences in age, gender, height, body mass index, fibular head height, JLCA, MPTA, and hip-knee-ankle angle among the five groups were statistically significant (P < 0.05), and the differences in body weight were not statistically significant (P > 0.05). There were significant differences in fibular head height, JLCA, JLCA and hip-knee-ankle angle between different groups (P < 0.05). Furthermore, there were significant differences in JLCA and hip-knee-ankle angle (P < 0.05), and both JLCA and hip-knee-ankle angle increased with severe aggravation of Kellgren-Lawrence grade. Furthermore, both fibular head height and MPTA decreased as the Kellgren-Lawrence grade was severely aggravated. There was a significant negative correlation between Kellgren-Lawrence grade and fibular head height (r = -0.812, P < 0.001). Furthermore, there was a significant negative correlation between fibular head height and hip-knee-ankle angle (r = -0.7905, P < 0.001). When Kellgren-Lawrence grade III and IV knees were considered as disease, ROC curve analysis showed a cut-off value of 10.63 for fibular head height and an AUC of 0.872.Conclusion: The height of fibular head in patients with varus knee osteoarthritis is smaller than that in non-osteoarthritis patients. In addition to body mass index, fibular head height is a risk factor for the pathogenesis of varus knee osteoarthritis,the smaller the fibular head height, the more severe the severity of osteoarthritis and the more severe the degree of varus deformity.