1. Glucocorticoids protect HEI-OC1 cells from tunicamycin-induced cell damage via inhibiting endoplasmic reticulum stress
- Author
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Wandong She, Ziwen Gao, Xiaorui Chen, Jin Liu, Lingyun Lv, Wei Ma, Zhibiao Liu, Bing Fei, Jie Hou, Lisheng Xie, and Wenyan Zhu
- Subjects
0301 basic medicine ,QH301-705.5 ,mifepristone ,dexamethasone ,CHOP ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,otorhinolaryngologic diseases ,medicine ,Biology (General) ,030223 otorhinolaryngology ,Protein kinase A ,Cell damage ,General Immunology and Microbiology ,Kinase ,General Neuroscience ,Endoplasmic reticulum ,Tunicamycin ,medicine.disease ,030104 developmental biology ,chemistry ,Apoptosis ,endoplasmic reticulum stress ,Cancer research ,PERK–CHOP pathway ,General Agricultural and Biological Sciences ,Glucocorticoid ,Research Article ,medicine.drug - Abstract
Background To analyze mechanisms of action of glucocorticoid treatment for endoplasmic reticulum stress (ERS) in sensorineural hearing loss (SNHL), we aimed to evaluate the expression and activation status of the protein kinase RNA-like ER kinase (PERK)–C/EBP homologous protein (CHOP) pathway, which is the major pathway in the ERS. Methods In the present study, we established an in vitro ERS model using tunicamycin-treated hair-cell-like HEI-OC1 cells. The effect of dexamethasone on proliferation inhibition, apoptosis, and ATF4–CHOP pathway in HEI-OC1 cells was examined by CCK-8 assay, flow cytometry, western blotting, and reverse transcription PCR, respectively. Results In HEI-OC1 cells, dexamethasone was shown to significantly reduce the tunicamycin-induced expression of ATF4 and CHOP in the context of sustained viability and proliferation, a therapeutic effect that was reversible by co-treatment with a glucocorticoid antagonist. Conclusion Dexamethasone can protect hair-cell-like HEI-OC1 cells from ERS damage, which may be one of the mechanisms of action for GCs in SNHL treatment.
- Published
- 2021