1. Magnetic implants in vivo guiding sorafenib liver delivery by superparamagnetic solid lipid nanoparticles
- Author
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Maria Lucia Curri, Angela Lopedota, Amalia Azzariti, Giusy Bianco, Ilaria Arduino, Gianluigi Giannelli, Valentino Laquintana, Fabio Canepa, Maria Principia Scavo, Nicoletta Depalo, Antonio Lopalco, Annalisa Cutrignelli, Byung Chul Lee, Massimo Franco, Nunzio Denora, Elisabetta Fanizza, Maria Notarnicola, Rosa Maria Iacobazzi, and Fabio Vischio
- Subjects
Biomaterials ,Mice ,Colloid and Surface Chemistry ,In vivo ,Solid lipid nanoparticle ,medicine ,Animals ,Magnetite Nanoparticles ,Chemistry ,Sorafenib ,equipment and supplies ,medicine.disease ,In vitro ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Magnetic Fields ,Liver ,Magnet ,Liposomes ,Drug delivery ,Nanoparticles ,Liver cancer ,human activities ,Superparamagnetism ,Homogenization (biology) ,Biomedical engineering - Abstract
Hypothesis Solid lipid nanoparticles (SLNs), co-encapsulating superparamagnetic iron oxide nanoparticles and sorafenib, have been exploited for magnetic-guided drug delivery to the liver. Two different magnetic configurations, both comprising two small magnets, were under-skin implanted to investigate the effect of the magnetic field topology on the magnetic SLNP accumulation in liver tissues. A preliminary simulation analysis was performed to predict the magnetic field topography for each tested configuration. Experiments SLNs were prepared using a hot homogenization approach and characterized using complementary techniques. Their in vitro biological behavior was assessed in HepG-2 liver cancer cells; wild-type mice were used for the in vivo study. The magnet configuration that resulted in a higher magnetic targeting efficiency was investigated by evaluating the iron content in homogenated murine liver tissues. Findings SLNs, characterized by an average size smaller than 200 nm, retained their superparamagnetic behavior and relevant molecular resonance imaging properties as negative contrast agents. The evaluation of iron accumulation in the liver tissues was consistent with the magnetic induction profile of each magnet configuration, concurring with the results predicted by simulation analysis and obtained by measurements in living mice.
- Published
- 2022