1. Impact of the severity of negative energy balance on gene expression in the subcutaneous adipose tissue of periparturient primiparous Holstein dairy cows: Identification of potential novel metabolic signals for the reproductive system
- Author
-
Theodoros Ntallaris, Yan Jaszczyszyn, Christelle Ramé, Daniel Guillaume, Namya Mellouk, Patrice Humblot, Jean-Luc Touzé, Joëlle Dupont, Delphine Naquin, Eric Briant, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Plateforme de séquençage à haut débit (NGS), Département Plateforme (PF I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Swedish University of Agricultural Sciences (SLU), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Physiology ,[SDV]Life Sciences [q-bio] ,Adipose tissue ,Gene Expression ,White adipose tissue ,Severity of Illness Index ,Biochemistry ,Epithelium ,Transcriptome ,Endocrinology ,Reproductive Physiology ,Animal Cells ,Lactation ,Gene expression ,Medicine and Health Sciences ,RNA-Seq ,Lipid Hormones ,Progesterone ,0303 health sciences ,Multidisciplinary ,04 agricultural and veterinary sciences ,Progesterone secretion ,Lipids ,Body Fluids ,Dairying ,medicine.anatomical_structure ,Blood ,Adipose Tissue ,Medicine ,Animal Nutritional Physiological Phenomena ,Female ,Anatomy ,Cellular Types ,Research Article ,Genetics and Breeding ,Science ,Subcutaneous Fat ,Adipokine ,Biology ,Blood Plasma ,Andrology ,03 medical and health sciences ,Metabolic Diseases ,Weight Loss ,medicine ,Genetics ,Animals ,Genitalia ,030304 developmental biology ,Granulosa Cells ,Chemokine CCL21 ,Endocrine Physiology ,Body Weight ,0402 animal and dairy science ,Biology and Life Sciences ,Lipid metabolism ,Epithelial Cells ,Cell Biology ,Lipid Metabolism ,040201 dairy & animal science ,Hormones ,Biological Tissue ,Metabolism ,Gene Expression Regulation ,Cattle ,Lutein Cells ,Energy Metabolism ,Biomarkers - Abstract
International audience; The severity of negative energy balance (NEB) in high-producing dairy cows has a high incidence among health diseases. The cow's energy status during early lactation critically affects metabolic and reproductive parameters. The first objective of this study was to investigate by RNA-seq analysis and RT-qPCR the gene expression profile in white adipose tissue and by gene ontology and upstream regulation tools the relationships with energy metabolism and reproduction in two groups of primiparous dairy cows with extreme NEB statuses (NEB \textless -9 Mcal/day vs. NEB \textgreater -9 Mcal/day) around parturition. The second objective was to determine the potential involvement of a new adipokine identified as a candidate for the regulation of ovarian function in our RNA-seq analysis by using bovine primary granulosa culture, thymidine incorporation to determine cell proliferation and ELISA assays to measure progesterone secretion. The RNA-seq analysis revealed that 514 genes were over-expressed and 695 were under-expressed in the adipose tissue of cows with severe NEB (SNEB) and cows with moderate NEB (MNEB) during the -4 and 16 wkpp period. In addition, 491 genes were over-expressed and 705 genes were under-expressed in the adipose tissue of SNEB cows compared to MNEB cows. Among these differently expressed genes (DEGs), 298 were related to metabolic functions and 264 to reproductive traits. A set of 19 DEGs were validated by RT-qPCR, including CCL21 (C-C motif chemokine ligand 21). Moreover, CCL21, a gene known to be secreted by adipose tissue, was chosen for further analysis in plasma and ovaries. The use of next-generation sequencing technologies allowed us to characterise the transcriptome of white adipose tissue from primiparous cows with different levels of NEB during lactation. This study highlighted the alteration of the expression of genes related to lipid metabolism, including CCL21, which is released in the bloodstream and associated with the in vitro regulation of ovarian functions.
- Published
- 2019