12 results on '"Yong Gyun Kim"'
Search Results
2. Antifungal Effect of Phenyllactic Acid Produced by Lactobacillus casei Isolated from Button Mushroom
- Author
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Yong-Gyun Kim, Byung-Eui Lee, Min-Ho Yoon, Chan-Jung Lee, and Jeoung Ah Yoo
- Subjects
0106 biological sciences ,Antifungal ,Lactobacillus casei ,Mushroom ,biology ,medicine.drug_class ,Chemistry ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,01 natural sciences ,Microbiology ,0404 agricultural biotechnology ,010608 biotechnology ,medicine - Published
- 2016
3. Paraneoplastic demyelinating polyneuropathy associated with cardiac myxoma
- Author
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Suho Ro, Yong Gyun Kim, Bum Chun Suh, Yun Hyeong Jeong, and In-Woo Park
- Subjects
Neurologic Examination ,Pathology ,medicine.medical_specialty ,Physiology ,business.industry ,Mononeuropathy Multiplex ,Myxoma ,medicine.disease ,Quadriplegia ,Heart Neoplasms ,Cellular and Molecular Neuroscience ,Treatment Outcome ,Embolism ,Physiology (medical) ,medicine ,Humans ,Paraneoplastic Polyneuropathy ,Female ,Neurology (clinical) ,Demyelinating polyneuropathy ,business ,Aged ,Demyelinating Diseases - Published
- 2019
4. Effects of Virtual Reality-Based Exercise on Balance, Gait, and Falls Efficacy in Patients with Parkinson's Disease: A pilot study
- Author
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Soon-Hee Kang and Yong-Gyun Kim
- Subjects
030506 rehabilitation ,medicine.medical_specialty ,Parkinson's disease ,business.industry ,Falls efficacy ,Virtual reality ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Gait (human) ,Physical medicine and rehabilitation ,Physical therapy ,Medicine ,In patient ,0305 other medical science ,business ,030217 neurology & neurosurgery ,Balance (ability) - Published
- 2016
5. Characterization and Production of Thermostable and Acid-stable Extracellular Fibrinolytic Enzymes from Cordyceps militaris
- Author
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Yong-Gyun Kim, Hong-Joo Son, Sang-Mong Lee, Byung-Wook Cho, Hyun Chul Park, Seon-Ah Kim, and Keun-Ki Kim
- Subjects
chemistry.chemical_classification ,Proteases ,Protease ,biology ,medicine.medical_treatment ,Proteolytic enzymes ,biology.organism_classification ,Enzyme assay ,chemistry.chemical_compound ,Enzyme ,Biochemistry ,chemistry ,Casein ,Cordyceps militaris ,biology.protein ,medicine ,PMSF - Abstract
Biochemical and enzymatic characterization for extracellular protease isolated from Cordyceps militaris cultivated on rice bran medium was investigated. C militaris produced proteolytic enzymes from 10 days after inoculation, maximum enzyme production was found at 25 days. The optimum temperature and pH of proteases production was at and pH 7.0, respectively. The protease activity was observed in the four peaks (Pro-I, Pro-II, Pro-III, and Pro-IV) separated through Sephadex G-100 column chromatography. The separated protease was optimally active at . Optimum pH of the protease was between 7 and 8. Enzyme was also stable over at . The enzyme was highly stable in a pH range of 4-9. Protease activity was found to be slightly decreased by the addition of , , , and , whereas inhibited by the addition of and Protease activity was inhibited by protease inhibitor PMSF. On the other hand, the partially purified protease was investigated on proteolytic protease activity by zymogram gel electrophoresis using three substances (casein, gelatin and fibrin). Four active bands (F-I, FII, F-III, and F-IV) of fibrin degradation were revealed on fibrin zymogram gels. Both of F-II and FIII showed caseinolytic, fibrinolytic and gelatinolytic activities in three gels. Thermostability, pH stability, and pH-thermostability of the enzyme determined the residual fibrinolytic activity also displayed on fibrin zymogram gel. The only one enzyme (F-II) displayed over a broad range of temperature at . The FII displayed fibrinolytic activity in the pH range 3-5, but was inactivated in the range of pH 6-11. The F-I and F-III showed enzyme activity in the pH range of 6-11. In the pH-thermostability, the F-II only kept fibrinolytic activity after heating at for 10, 20 and 30 min at pH 3 and pH 7, respectively. On the other hand, the F-II was retained activity until heating for 10 min under pH 11 condition. By using fibrin zymogram gel electrophoresis, extracellular fibrinolytic enzyme F-II from C. militaris showed unusual thermostable under acid and neutral conditions.
- Published
- 2011
6. Optimal Condition to Produce Protease by Strain Separated from the Intestine of Reticulitermes speratus
- Author
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Keun-Ki Kim, Sang-Mong Lee, Yong-Gyun Kim, Hyean-Cheal Park, Min-Kyung Park, and Hong-Joo Son
- Subjects
chemistry.chemical_classification ,Protease ,Strain (chemistry) ,Starch ,medicine.medical_treatment ,Reticulitermes speratus ,Biology ,biology.organism_classification ,16S ribosomal RNA ,Microbiology ,chemistry.chemical_compound ,Enzyme ,chemistry ,Serratia marcescens ,medicine ,Bacteria - Abstract
We separated the bacteria showing protease activity from Reticulitermes speratus which is known as the only termite species in Korea. Then, we collected the best activated strain and studied the optimal culture condition for producing the enzyme. According to the results of observing morphological and physiological characteristics, and the type of 16S rRNA of the strain, it was identified as Serratia marcescens and named S. marcescens strain TM-3215. This strain showed the best activity under conditions of 0.8% (w/v) starch, 0.4% (w/v) peptone, 0.08% (w/v) MgSO₄?7H₂O, 30℃ and pH 8.0. After being cultivated under optimal conditions for 9 hr, the strain produced 19.8 U/ml of enzyme, an amount 1.8 times greater than the control.
- Published
- 2010
7. A Case of Catheter-Related Bacteremia of Tsukamurella pulmonis
- Author
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Gyu Hyung Lee, Seung Namgung, Yong Gyun Kim, Heungsup Sung, Hyoeun Shim, Seung Mi Baek, and Mi Na Kim
- Subjects
Adult ,Male ,food.ingredient ,medicine.drug_class ,Clinical Biochemistry ,Antibiotics ,Bacteremia ,Biology ,medicine.disease_cause ,Vial ,Meropenem ,Microbiology ,food ,RNA, Ribosomal, 16S ,Amphotericin B ,Actinomycetales ,medicine ,Humans ,Agar ,Phylogeny ,Bone Marrow Transplantation ,Tsukamurella pulmonis ,Biochemistry (medical) ,General Medicine ,medicine.disease ,Leukemia, Myeloid, Acute ,Catheter-Related Infections ,Vancomycin ,Actinomycetales Infections ,medicine.drug - Abstract
Tsukamurella pulmonis is an aerobic actinomycete. We report a catheter-related bacteremia of T. pulmonis. A 39 yr-old male with ALL was hospitalized to receive bone marrow transplantation (BMT). Although the patient developed a high fever at the 7th hospital day (HD), it subsided with vancomycin treatment, and he received BMT at 9th HD. Fever resurged at 16th HD despite sustained treatment with vancomycin, meropenem, and amphotericin B, but subsided with removal of Hickman catheter (HC) at 19th HD. Three sets of blood cultures comprising one from the HC and two from venipunctures were taken at 7th, 16th, and 19th HD, and the distal tip of the HC was also cultured. The aerobic vials of all 3 HC-withdrawn blood cultures and one peripheral blood culture taken at 19HD and the HC tip culture grew long, straight, thin gram-positive rods that were positive on modified Kinyoun stain. This organism showed tiny, rough, grey colonies after 3-day incubation and grew to large flat colonies when incubation was extended. It was catalase-positive, urease-positive, and alkaline-slant/alkaline-deep on triple sugar iron agar, and hydrolyzed hypoxanthine. The sequence of 1,296 base pairs of 16S rRNA of this organism showed a 100.0% homology with the published sequence of T. pulmonis DSM 44142T. To our knowledge, this is the first report of T. pulmonis bacteremia in Korea.
- Published
- 2009
8. Responses of Plasma IGF-1, IGFBPs and Hepatic GH Receptor to Growth Hormone Releasing Peptides (GHRP)-2 Administration and Energy Level in Wethers
- Author
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Hyun Chul Park, Hisashi Hidari, Yong Gyun Kim, Keun Ki Kim, Han Seok Kang, Sang Mong Lee, Seon Ku Kim, Young Cheng Jin, Yun-Jaie Choi, Hong-Gu Lee, Teak Soon Shin, Hong Joo Son, and Byung Uuk Cho
- Subjects
medicine.medical_specialty ,GH Receptor ,Chemistry ,Growth factor ,medicine.medical_treatment ,Significant difference ,Endogeny ,Growth hormone ,Endocrinology ,Internal medicine ,medicine ,Dry matter ,Receptor ,Saline - Abstract
The purpose of this study was to determine the effect of energy supplement on responses of plasma insulin-like growth factor (IGF)-1 and IGF binding proteins (IGFBPs) to growth hormone-releasing peptide-2 (GHRP-2) administration in normal protein-fed wethers, and to observe the effect of GHRP-2 treatment on hepatic growth hormone (GH) receptor in well-fed wethers. Plasma IGF-1 and 39-42 kDa IGFBP-3 during the HENP (CP, crude protein 0.34 and TDN, total digestible nutrients 1.83 ㎏/day DM, dry matter intake) treatment period were higher than in the LENP (CP 0.32 ㎏ and TDN 0.87 ㎏/day DM intake) period (P<0.05). The response of GH was stimulated by GHRP-2 (12.5 ㎍/㎏ body weight/day) administration during both of the feed treatment periods (P<0.05). The area under curve (AUC) increment and average concentration of GH (0-180 min) with GHRP-2 administration was higher during HENP treatment than LENP treatment (P<0.01). During the HENP treatment period from day 1 to day 7 of twice daily GHRP-2 treatment, the plasma IGF-1 increment was increased on days 2, 6 and 7 of GHRP-2 administration (P<0.05). On the basis of ligand blotting, the proportions of plasma 39-43 kDa IGFBP-3 during the HENP treatment period only showed a significant difference on days 6 and 7 with GHRP-2 administration. No significant difference in the specific binding of 125I-labeled oGH to hepatic membranes was detected between the saline and GHRP-2 treatments of the HENP-fed wethers. These results suggest that the nutritional balance between energy and protein may affect the endogenous GH / IGF-1 axis as well as plasma IGFBP-3 levels.
- Published
- 2008
9. Antimicrobial and Anticancer Activity of Korean Traditional Soy Sauce and Paste with Chopi
- Author
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Young-Whan Choi, Yong-Gyun Kim, Insoo Choi, Sang-Mong Lee, Teak-Soon Shin, Hong-Joo Son, Hyean-Cheal Park, and Keun-Ki Kim
- Subjects
biology ,medicine.drug_class ,Chemistry ,Antibiotics ,Antimicrobial ,biology.organism_classification ,medicine.disease_cause ,Ingredient ,Biochemistry ,Staphylococcus aureus ,medicine ,DNA fragmentation ,Food science ,Escherichia coli ,Bacteria ,Zanthoxylum piperitum - Abstract
The fruits of Zanthoxylum piperitum are known as having various physiology vitality, and the abstraction ingredient of the pericarp is also known as having strong antibiotic activities against various bacteria. Therefore, this study was carried out to estimate the effect of physiology vitality when the abstraction ingredient of Z. piperitum was added in soy sauce(Chopi-kanjang) and soybean paste(Chopi-doenjang). For the antibiotic activity against the pathogens of sitotoxism such as Staphylococcus aureus, Salmonella typhimurium, Vibrio parahemolyticus, Escherichia coli 0157:H7, the extracts of the Chopi-kanjang was added 1%, 2%, 4% pericarp of Z. piperitum in the manufacturing process of soy source. According to the results, the growth of E. coli 0157:H7 and V. parahemolyticus were respectively inhibited as 70% and 50% by the Chopi-kanjang added 2% of the ingredient. For the antibiotic effects of the aforementioned Chopi-kanjang against Sal. typhimurium and Sta. aureus, the growth of those pathogens was also inhibited between 40% and 60% according to the manufacturing period of Chopi-kanjang. It was confirmed that the antibiotic activity using the mixture of the abstraction ingredient and Chopi-doenjang was lower than those of Chopi-kanjang. In order to estimate the anticancer activity using by caspase-3 activity, the mixture of the abstraction ingredient of the pericarp of Z. piperitum and Chopi-kanjang was treated to leukemia cells. According to the results, the activities of caspase-3 using the mixture added 1%, 2% and 4% of the abstraction ingredient were respectively increased as much as 4, 12, 15 times comparing with the control which was treated with the soy source only. It could be that the mixture of the abstraction ingredient of the pericarp of Z. piperitum and soy source induced apoptosis, and the mixture of the abstraction ingredient and soybean paste had no effect on the activity of caspase-3. In order to find out the death of the aforementioned cells caused by necrosis or apoptosis, DNA fragmentation in the cell was examined. U-937 cells showed apoptotic DNA fragmentation in the incubation with Chopi-kanjang extract.
- Published
- 2007
10. Exogenous JH and ecdysteroid applications alter initiation of polydnaviral replication in an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera)
- Author
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Yong Gyun Kim and Bok Ri Park
- Subjects
Agonist ,medicine.drug_class ,Molecular Sequence Data ,Wasps ,Virus Replication ,Biochemistry ,chemistry.chemical_compound ,Viral Proteins ,medicine ,Animals ,Molecular Biology ,Ecdysteroid ,biology ,Base Sequence ,Polydnavirus ,Pupa ,Ecdysteroids ,General Medicine ,biology.organism_classification ,Virology ,Juvenile Hormones ,Viral replication ,chemistry ,Polydnaviridae ,Juvenile hormone ,Female ,Braconidae ,Sequence Alignment ,DNA ,Hormone - Abstract
Polydnaviruses are a group of double-stranded DNA viruses and are symbiotically associated with some ichneumonoid wasps. As proviruses, the replication of polydnaviruses occurs in the female reproductive organ at the pupal stage. This study analyzed the effects of two developmental hormones, juvenile hormone (JH) and ecdysteroid, on the viral replication of Cotesia plutellae bracovirus (CpBV). All 23 CpBV segments identified contained a conserved excision/rejoining site ('AGCTTT') from their proviral segments. Using quantitative real-time PCR based on this excision/rejoining site marker, initiation of CpBV replication was determined to have occurred on day 4 on the pupal stage. Pyriproxyfen, a JH agonist, significantly inhibited adult emergence of C. plutellae, whereas RH5992, an ecdysteroid agonist, had no inhibitory effect. Although RH5992 had no effect dose on adult development, it significantly accelerated viral replication. The results of immunoblotting assays against viral coat proteins support the effects of the hormone agonists on viral replication.
- Published
- 2011
11. Inhibition of GSK-3β enhances reovirus-induced apoptosis in colon cancer cells
- Author
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Il-Rae Cho, Young-Hwa Chung, Hye-Jin Min, Ratakorn Srisuttee, Eun Hee Park, Yong-Gyun Kim, Sangtaek Oh, Randal N. Johnston, Byung Hak Jhun, Ju Eun Kwak, and Sang Seok Koh
- Subjects
Cancer Research ,Programmed cell death ,Oncogene ,Cancer ,Cell cycle ,Biology ,Caspase 8 ,medicine.disease ,Oncolytic virus ,Oncology ,Apoptosis ,GSK-3 ,Immunology ,Cancer research ,medicine - Abstract
Reovirus functions as an oncolytic agent for many types of cancer including colon cancer. Although most studies have emphasized the role of activated Ras signaling in enhancing reoviral oncolysis in susceptible cells, we note that many colon cancers also display elevated beta-catenin. Thus, it is possible that enhanced beta-catenin may augment reoviral susceptibility in colon cancer cells. To explore this hypothesis, HEK293 cells were treated with the glycogen synthase kinase (GSK)-3beta inhibitor LiCl, thereby inducing beta-catenin, followed by reoviral infection. Co-administration with LiCl indeed enhanced cell death compared to reovirus infection alone, but this was not associated with elevated reoviral replication. Similarly, HEK293 cells expressing the Frizzled-1 receptor in Wnt3a-conditioned medium also showed reovirus replication equivalent to that in cells in control medium, further suggesting that up-regulation of beta-catenin does not enhance the replication of reovirus. Instead, we observed that inhibition of GSK-3beta with LiCl decreased reovirus-induced NF-kappaB activation, leading to accelerated apoptosis via caspase 8 activation. We further found that colon cancer HCT116 cells were sensitized to apoptosis by co-treatment with reovirus and a GSK-3beta inhibitor, AR-A014418. Finally, we identified that inhibition of NF-kappaB sensitized apoptosis of HEK293 or HCT 116 cells during reovirus infection. Taken together, we propose that inhibition of GSK-3beta sensitizes reovirus-induced apoptosis of colon cancer cells by down-regulation of NF-kappaB activity, offering a potentially improved therapeutic strategy for the treatment of colon cancer.
- Published
- 2009
12. Effect Of 8 Weeks Pilates For The Muscle Strength, Balance And Cardiorespiratory Function In Archers
- Author
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Jae Sung Joh, Ki Jae Song, Yong Gyun Kim, Jae-Keun Oh, and Da Woon Park
- Subjects
medicine.medical_specialty ,Physical medicine and rehabilitation ,business.industry ,Physical therapy ,Muscle strength ,Medicine ,Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,Cardiorespiratory fitness ,business ,Balance (ability) - Published
- 2015
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