35 results on '"Teng, Yue"'
Search Results
2. Collectin-11 promotes cancer cell proliferation and tumor growth
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Jia-Xing Wang, Bo Cao, Ning Ma, Kun-Yi Wu, Wan-Bing Chen, Weiju Wu, Xia Dong, Cheng-Fei Liu, Ya-Feng Gao, Teng-Yue Diao, Xiao-Yun Min, Qing Yong, Zong-Fang Li, Wuding Zhou, and Ke Li
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Immunology ,Oncology ,Medicine - Abstract
Collectin-11 (CL-11) is a recently described soluble C-type lectin that has distinct roles in embryonic development, host defence, autoimmunity, and fibrosis. Here we report that CL-11 also plays an important role in cancer cell proliferation and tumor growth. Melanoma growth was found to be suppressed in Colec11–/– mice in a s.c. B16 melanoma model. Cellular and molecular analyses revealed that CL-11 is essential for melanoma cell proliferation, angiogenesis, establishment of more immunosuppressive tumor microenvironment, and the reprogramming of macrophages to M2 phenotype within melanomas. In vitro analysis revealed that CL-11 can activate tyrosine kinase receptors (EGFR, HER3) and ERK, JNK, and AKT signaling pathways and has a direct stimulatory effect on murine melanoma cell proliferation. Furthermore, blockade of CL-11 (treatment with L-fucose) inhibited melanoma growth in mice. Analysis of open data sets revealed that COLEC11 gene expression is upregulated in human melanomas and that high COLEC11 expression has a trend toward poor survival. CL-11 also had direct stimulatory effects on human tumor cell proliferation in melanoma and several other types of cancer cells in vitro. Overall, our findings provide the first evidence to our knowledge that CL-11 is a key tumor growth–promoting protein and a promising therapeutic target in tumor growth.
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- 2023
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3. Home-based spirometry in the self-management of chronic obstructive pulmonary disease
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Teng-Yue Zhang, Yu-Di He, Ke-Qi Chen, Yang Zhao, Yi-Xin Zhao, Kai-Feng Xu, and Pei-Fang Wei
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Medicine - Published
- 2021
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4. Time Regularity of Morphology of Blood Pools
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ZHENG Ji-long, ZHAO Kai-fang, TENG Yue,et al
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forensic pathology ,blood pool ,morphology ,Medicine - Abstract
Objective To provide reference indexes and theoretical basis for age estimation of blood pools by investigating the entire drying process and monitoring the change of morphology and mass. Methods Four 15 mL blood pool samples were prepared on the clean ceramic plate. The change of morphology and mass of blood pools in a closed dark environment with a temperature of (20.0±0.5) ℃ and a humidity of 35%-45% were dynamically observed from 0 h to 60 h. Images of the blood pools were recorded by digital camera. The area of blood pools was calculated by MATLAB R2014b, the length of cracks was measured by Image J and the statistical analysis was performed by SPSS 16.0. Results By summarizing and analyzing, the drying of blood pools was divided into five stages: coagulation (0-4.5 h), gelation (>4.5-20.0 h), gel-solid mixing (>20.0-37.0 h), solid (>37.0-40.0 h) and final desiccation (>40.0-45.0 h). From 0 to 45 h, the mass of the blood pools decreased linearly with time, and the decrease was not obvious from 45.0 to 60.0 h. The standardized mass (y2) showed strong correlation with the time (x) y2=0.018 2 x+0.271 4(R2=0.967 9). The area change rate of blood pools, the distance that the edge of blood pools moved, the average length of radical cracks had little correlation with the time that passed. Conclusion The overall morphological characteristics of blood pools show a certain regularity with the time and the standardized indexes established provide a reference for the age estimation of blood pools.
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- 2020
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5. The Short-Term Patency Rate of a Saphenous Vein Bridge Using the No-Touch Technique in off-Pump Coronary Artery Bypass Grafting in Vein Harvesting
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Yu Liu, Ji-Qiang Bu, Zi-Ying Chen, Jian-Jun Gu, Teng-Yue Zhao, and Wen-Li Zhang
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medicine.medical_specialty ,medicine.medical_treatment ,International Journal of General Medicine ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Left coronary artery ,great saphenous vein ,medicine.artery ,medicine ,Vein ,Original Research ,Off-pump coronary artery bypass ,business.industry ,Great saphenous vein ,No touch technique ,General Medicine ,Surgery ,medicine.anatomical_structure ,Bridge (graph theory) ,left internal mammary artery ,030220 oncology & carcinogenesis ,Right coronary artery ,off-pump coronary artery bypass grafting ,no-touch technique ,business ,Artery - Abstract
Teng-Yue Zhao,* Ji-Qiang Bu,* Jian-Jun Gu, Yu Liu, Wen-Li Zhang, Zi-Ying Chen Department of Cardiac Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zi-Ying ChenDepartment of Cardiac Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, People’s Republic of ChinaTel +86 15803210520Fax +86 3116188269Email chenzy_de@163.comObjective: This study aimed to examine the short-term effect of the no-touch technique on the patency rate of a great saphenous vein (GSV) bridge used during off-pump coronary artery bypass grafting (OPCABG).Methods: Between June 2018 and September 2020, 140 patients undergoing OPCABG, with grafts obtained from the GSV using the “no-touch” technique or the left internal mammary artery (LIMA), were enrolled in this study. The early clinical results and short-term patency rate of the OPCABG were evaluated at a three-month follow-up by comparing the patency rate of the LIMA bridge and the GSV bridge obtained by the no-touch technique. This study also analyzed the impacts of the postoperative complications of the lower limbs and the distribution area of diseased vessels on the patency rate of a GSV bridge obtained by the no-touch technique at an early stage.Results: No perioperative death or adverse cardiovascular or cerebrovascular events occurred in the 140 patients undergoing OPCABG. The difference in the early patency rate between the GSV bridge obtained by the no-touch technique and the LIMA bridge was not statistically significant (95.9% vs 97.1%, p = 0.501). There was no significant difference in the patency rate between an end-to-side anastomosed venous bridge and a LIMA bridge (95.0% [248/261] vs 97.1% [136/140], p = 0.314). The overall patency rate of a no-touch vein bridge in the right coronary artery region was lower than it was in the left coronary artery region (93.8% [165/176] vs 97.9% [183/187], p = 0.049).Conclusion: The no-touch technique may improve the early patency rate of a GSV bridge, and its effect is similar to that of a LIMA bridge.Keywords: off-pump coronary artery bypass grafting, no-touch technique, great saphenous vein, left internal mammary artery
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- 2021
6. Cytisine Exerts an Anti-Epileptic Effect via α7nAChRs in a Rat Model of Temporal Lobe Epilepsy
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Jing-jun Zheng, Teng-yue Zhang, Hong-tao Liu, Ze-xin Huang, Jing-mei Teng, Jing-xian Deng, Jia-gui Zhong, Xu Qian, Xin-wen Sheng, Ji-qiang Ding, Shu-qiao He, Xin Zhao, Wei-dong Ji, De-feng Qi, Wei Li, and Mei Zhang
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0301 basic medicine ,Agonist ,medicine.drug_class ,cytisine ,RM1-950 ,Pharmacology ,behavioral disciplines and activities ,Neuroprotection ,Partial agonist ,03 medical and health sciences ,Epilepsy ,Cytisine ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,Pharmacology (medical) ,cholinergic transmission ,Original Research ,synaptic remodeling ,business.industry ,Glutamate receptor ,temporal lobe epilepsy ,medicine.disease ,α7nAChRs ,030104 developmental biology ,chemistry ,nervous system ,Cholinergic ,neuroprotection ,Therapeutics. Pharmacology ,business ,psychological phenomena and processes ,030217 neurology & neurosurgery ,Acetylcholine ,medicine.drug - Abstract
Background and Purpose: Temporal lobe epilepsy (TLE) is a common chronic neurological disease that is often invulnerable to anti-epileptic drugs. Increasing data have demonstrated that acetylcholine (ACh) and cholinergic neurotransmission are involved in the pathophysiology of epilepsy. Cytisine, a full agonist of α7 nicotinic acetylcholine receptors (α7nAChRs) and a partial agonist of α4β2nAChRs, has been widely applied for smoking cessation and has shown neuroprotection in neurological diseases. However, whether cytisine plays a role in treating TLE has not yet been determined.Experimental Approach: In this study, cytisine was injected intraperitoneally into pilocarpine-induced epileptic rats for three weeks. Alpha-bungarotoxin (α-bgt), a specific α7nAChR antagonist, was used to evaluate the mechanism of action of cytisine. Rats were assayed for the occurrence of seizures and cognitive function by video surveillance and Morris water maze. Hippocampal injuries and synaptic structure were assessed by Nissl staining and Golgi staining. Furthermore, levels of glutamate, γ-aminobutyric acid (GABA), ACh, and α7nAChRs were measured.Results: Cytisine significantly reduced seizures and hippocampal damage while improving cognition and inhibiting synaptic remodeling in TLE rats. Additionally, cytisine decreased glutamate levels without altering GABA levels, and increased ACh levels and α7nAChR expression in the hippocampi of TLE rats. α-bgt antagonized the above-mentioned effects of cytisine treatment.Conclusion and Implications: Taken together, these findings indicate that cytisine exerted an anti-epileptic and neuroprotective effect in TLE rats via activation of α7nAChRs, which was associated with a decrease in glutamate levels, inhibition of synaptic remodeling, and improvement of cholinergic transmission in the hippocampus. Hence, our findings not only suggest that cytisine represents a promising anti-epileptic drug, but provides evidence of α7nAChRs as a novel therapeutic target for TLE.
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- 2021
7. Protective Role of Collectin 11 in a Mouse Model of Rheumatoid Arthritis
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Ke Li, Jia-Xing Wang, Nirmal K. Banda, Ang Li, Na Wang, Teng-Yue Diao, Dan Liu, Ning Ma, Jia-Yu Liu, Zongfang Li, Conrad A. Farrar, Rafael Bayarri-Olmos, Cui Qiang, Wuding Zhou, Xiao Yang, Li Xue, Weiju Wu, Peter Garred, Baojun Zhang, and Kun-Yi Wu
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Adult ,Male ,T cell ,T-Lymphocytes ,Immunology ,Arthritis ,Collectin ,Antigen-Presenting Cells ,Inflammation ,Blood Sedimentation ,Adaptive Immunity ,Severity of Illness Index ,Article ,Proinflammatory cytokine ,Arthritis, Rheumatoid ,Mice ,Immune system ,Rheumatology ,medicine ,Immunology and Allergy ,Animals ,Humans ,medicine.diagnostic_test ,business.industry ,medicine.disease ,Arthritis, Experimental ,Collectins ,Mice, Inbred C57BL ,Disease Models, Animal ,medicine.anatomical_structure ,C-Reactive Protein ,Erythrocyte sedimentation rate ,Rheumatoid arthritis ,Cytokines ,Female ,medicine.symptom ,business - Abstract
Objective: Collectin 11 (CL-11) is a soluble C-type lectin, a mediator of innate immunity. Its role in autoimmune disorders is unknown. We undertook this study to determine the role of CL-11 in a mouse model of rheumatoid arthritis (RA). Methods: A murine collagen-induced arthritis (CIA) model was used and combined two approaches, including gene deletion of Colec11 and treatment with recombinant CL-11 (rCL-11). Joint inflammation and tissue destruction, circulating levels of inflammatory cytokines, and adaptive immune responses were assessed in mice with CIA. Splenic CD11c+ cells were used to examine the influence of CL-11 on antigen-presenting cell (APC) function. Serum CL-11 levels in RA patients were also examined. Results: Colec11 −/− mice developed more severe arthritis than wild-type mice, as determined by disease incidence, clinical arthritis scores, and histopathology (P < 0.05). Disease severity was associated with significantly enhanced APC activation, Th1/Th17 responses, pathogenic IgG2a production and joint inflammation, as well as elevated circulating levels of inflammatory cytokines. In vitro analysis of CD11c+ cells revealed that CL-11 is critical for suppression of APC activation and function. Pharmacologic treatment of mice with rCL-11 reduced the severity of CIA in mice. Analysis of human blood samples revealed that serum CL-11 levels were lower in RA patients (n = 51) compared to healthy controls (n = 53). Reduction in serum CL-11 was inversely associated with the Disease Activity Score in 28 joints, erythrocyte sedimentation rate, and C-reactive protein level (P < 0.05). Conclusion: Our findings demonstrate a novel role of CL-11 in protection against RA, suggesting that the underlying mechanism involves suppression of APC activation and subsequent T cell responses.
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- 2021
8. Protective Role of C3aR (C3a Anaphylatoxin Receptor) Against Atherosclerosis in Atherosclerosis-Prone Mice
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Jia-Xing Wang, Kun-Yi Wu, Shu-Juan Zhao, Ke Li, Liang Bai, Enqi Liu, Daxin Chen, Teng-Yue Diao, Zongfang Li, Ning Ma, Wuding Zhou, and Lin-Lin Wei
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0301 basic medicine ,Male ,Apolipoprotein B ,Mice, Knockout, ApoE ,Aortic Diseases ,Inflammation ,030204 cardiovascular system & hematology ,Receptors, G-Protein-Coupled ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Macrophage ,Animals ,Anaphylatoxin ,Receptor ,Aorta ,Cells, Cultured ,Chemokine CCL2 ,biology ,business.industry ,Tumor Necrosis Factor-alpha ,Chemotaxis ,TOR Serine-Threonine Kinases ,NF-kappa B ,Atherosclerosis ,Plaque, Atherosclerotic ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,Phenotype ,Immunology ,biology.protein ,Macrophages, Peritoneal ,medicine.symptom ,Inflammation Mediators ,Cardiology and Cardiovascular Medicine ,business ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Objective: Emerging evidence suggests that C3aR (C3a anaphylatoxin receptor) signaling has protective roles in various inflammatory-related diseases. However, its role in atherosclerosis has been unknown. The purpose of the study was to investigate the possible protective role of C3aR in aortic atherosclerosis and explore molecular and cellular mechanisms involved in the protection. Approach and Results: C3ar −/− /Apoe −/− mice were generated by cross-breeding of atherosclerosis-prone Apoe −/− mice and C3ar −/− mice. C3ar −/− /Apoe −/− mice and Apoe −/− mice (as a control) underwent high-fat diet for 16 weeks were assessed for (1) atherosclerotic plaque burden, (2) aortic tissue inflammation, (3) recruitment of CD11b + leukocytes into atherosclerotic lesions, and (4) systemic inflammatory responses. Compared with Apoe −/− mice, C3ar −/− /Apoe −/− mice developed more severe atherosclerosis. In addition, C3ar −/− /Apoe −/− mice have increased local production of proinflammatory mediators (eg, CCL2 [chemokine (C-C motif) ligand 2], TNF [tumor necrosis factor]-α) and infiltration of monocyte/macrophage in aortic tissue, and their lesional macrophages displayed an M1-like phenotype. Local pathological changes were associated with enhanced systemic inflammatory responses (ie, elevated plasma levels of CCL2 and TNF-α, increased circulating inflammatory cells). In vitro analyses using peritoneal macrophages showed that C3a stimulation resulted in upregulation of M2-associated signaling and molecules, but suppression of M1-associated signaling and molecules, supporting the roles of C3a/C3aR axis in mediating anti-inflammatory response and promoting M2 macrophage polarization. Conclusions: Our findings demonstrate a protective role for C3aR in the development of atherosclerosis and suggest that C3aR confers the protection through C3a/C3aR axis–mediated negative regulation of proinflammatory responses and modulation of macrophage toward the anti-inflammatory phenotype.
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- 2020
9. A Novel Set of Immune-associated Gene Signature predicts Biochemical Recurrence in Localized Prostate Cancer Patients after Radical Prostatectomy
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Tongtong Zhang, Jiaochen Luan, Teng-yue Zeng, Ninghong Song, Kai Zhao, Xiang Zhou, Qijie Zhang, Jiadong Xia, and Liangyu Yao
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Oncology ,Biochemical recurrence ,medicine.medical_specialty ,Proportional hazards model ,breakpoint cluster region ,Nomogram ,Biology ,Gene signature ,medicine.disease ,Prostate cancer ,Internal medicine ,medicine ,KEGG ,Survival analysis - Abstract
Background: Decision-making regarding biochemical recurrence (BCR) in localized prostate cancer (PCa) patients after radical prostatectomy (RP) mainly relies on clinicopathological parameters with a low predictive accuracy. Currently, accumulating evidence suggests that immune-associated genes (IAGs) play irreplaceable roles in tumorigenesis, progression and metastasis. Considering the critical role of immune in PCa, we therefore attempted to identify the novel IAGs signature and validate its prognostic value that can better forecast the risk for BCR and guide clinical treatment. Methods: RNA-sequencing and corresponding clinicopathological data were downloaded from the Gene Expression Omnibus (GEO) database and the Cancer Genome Atlas (TCGA) database. Weighted gene co-expression network analysis (WGCNA) was utilized to screen out the candidate module closely related to BCR, and univariate and LASSO Cox regression analyses were performed to build the gene signature. Kaplan-Meier (KM) survival analysis, time-dependent receiver operating curve (ROC), independent prognostic analysis and nomogram were also applied to evaluate the prognostic value of the signature. Besides, Gene ontology analysis (GO), Kyoto encyclopedia of genes and genomes (KEGG) and gene set enrichment analysis (GSEA) were used to explore potential biological pathways. Results: A total of six IAGs (SSTR1, NFATC3, NRP1, TUBB3, IL1R1, GDF15) were eventually identified and used to establish a novel IAGs signature. The Kaplan-Meier analysis revealed that patients with low-risk scores had longer recurrence-free survival (RFS) than those with high-risk scores in both GSE70769 and TCGA cohorts. Further, our signature was also proven to be a valuable independent prognostic factor for BCR. We also constructed a nomogram based on the gene signature and related clinicopathologic features, which excellently predict 1-year, 3-year and 5-year prognosis of localized PCa patients after RP. Moreover, functional enrichment analysis demonstrated the vital biological processes, and stratified GSEA revealed that a crucial immune-related pathway (T cell receptor signaling pathway) was notably enriched in the high-risk group. Conclusions: We successfully developed a novel robust IAGs signature that is powerful in BCR prediction in localized PCa patients after RP, and created a prognostic nomogram. In addition, the signature might help clinicians in selecting high-risk subpopulation, predicting survival status of patients and promoting more individualized therapies than traditional clinical factors.
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- 2020
10. Lactobacillus plantarum LP104 ameliorates hyperlipidemia induced by AMPK pathways in C57BL/6N mice fed high-fat diet
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Yiying Chen, Wang Yuhua, Chunhong Piao, Yu Wang, Wuyang Guan, Teng Yue, and Yuan Tian
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0301 basic medicine ,AMPK ,medicine.medical_specialty ,Antioxidant ,medicine.medical_treatment ,Medicine (miscellaneous) ,C57bl 6n ,Lactobacillus plantarum LP104 ,Liver injury ,medicine.disease_cause ,03 medical and health sciences ,0404 agricultural biotechnology ,Internal medicine ,Hyperlipidemia ,medicine ,TX341-641 ,030109 nutrition & dietetics ,Nutrition and Dietetics ,biology ,Chemistry ,Nutrition. Foods and food supply ,nutritional and metabolic diseases ,food and beverages ,04 agricultural and veterinary sciences ,CYP2E1 ,biology.organism_classification ,medicine.disease ,040401 food science ,Blot ,Endocrinology ,bacteria ,lipids (amino acids, peptides, and proteins) ,Lactobacillus plantarum ,Oxidative stress ,Food Science - Abstract
Lactobacillus plantarum LP104 was isolated with excellent antioxidant properties from kimchi. In order to evaluate the role of LP104 in improving hyperlipidemia in high-fat-diet mice, C57BL/6N mice were randomly assigned into three groups treated with different diets: normal chow (Control), HFD, and HFD with L. plantarum LP104. After eight weeks, L. plantarum LP104 treatment significantly reduced HFD-induced body weight gain and the levels of serum or liver TC, TG, LDL, ALT, AST, LPS and TNF-α, and elevated liver HDL levels. The liver lipid accumulation was decreased in L. plantarum LP104 group. L. plantarum LP104 could activate the AMPK/Nrf2/CYP2E1 related pathway and regulate expressions of related proteins by using western blotting. Therefore, L. plantarum LP104 will improve hyperlipidemia, liver metabolic disorders, and liver oxidative stress response.
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- 2020
11. Hypertension, Microvascular Pathology, and Prognosis After an Acute Myocardial Infarction
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Ian Ford, Colin Berry, Vannesa Teng Yue May, Ify Mordi, Jaclyn Carberry, David Carrick, Keith G. Oldroyd, Paul Welsh, Aleksandra Radjenovic, Naveed Sattar, Stuart Hood, Mitchell Lindsay, Annette Maznyczka, Andrew Davie, Kenneth Mangion, Nadeem Ahmed, Margaret McEntegart, Hany Eteiba, Stuart Watkins, Caroline Haig, Mark C. Petrie, Kirsty Wetherall, and Ahmed Mahrous
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Male ,medicine.medical_specialty ,hypertension ,030204 cardiovascular system & hematology ,Microvascular injury ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Internal Medicine ,medicine ,Humans ,In patient ,030212 general & internal medicine ,Myocardial infarction ,Aged ,business.industry ,Age Factors ,Heart ,Original Articles ,Middle Aged ,reperfusion injury ,Prognosis ,medicine.disease ,Magnetic Resonance Imaging ,Pathophysiology ,myocardial infarction ,Logistic Models ,Microvessels ,Multivariate Analysis ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Cardiology ,ST Elevation Myocardial Infarction ,Female ,atherosclerosis ,business ,Reperfusion injury - Abstract
Supplemental Digital Content is available in the text., The rationale for our study was to investigate the pathophysiology of microvascular injury in patients with acute ST-segment–elevation myocardial infarction in relation to a history of hypertension. We undertook a cohort study using invasive and noninvasive measures of microvascular injury, cardiac magnetic resonance imaging at 2 days and 6 months, and assessed health outcomes in the longer term. Three hundred twenty-four patients with acute myocardial infarction (mean age, 59 [12] years; blood pressure, 135 [25] / 79 [14] mm Hg; 237 [73%] male, 105 [32%] with antecedent hypertension) were prospectively enrolled during emergency percutaneous coronary intervention. Compared with patients without antecedent hypertension, patients with hypertension were older (63 [12] years versus 57 [11] years; P
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- 2018
12. Baicalein improves cognitive deficits and hippocampus impairments in temporal lobe epilepsy rats
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Teng-yue Zhang, Jing-jun Zheng, Zhao-Rui Wang, Mei Zhang, Xu Qian, Wei Li, Ji-qiang Ding, and Jia-gui Zhong
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0301 basic medicine ,Male ,Hippocampus ,Pharmacology ,medicine.disease_cause ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Epilepsy ,0302 clinical medicine ,Cognition ,Status Epilepticus ,Corticosterone ,Neurons ,General Neuroscience ,Brain ,Electroencephalography ,Neurodegenerative Diseases ,Temporal Lobe ,Neuroprotective Agents ,Flavanones ,Glucocorticoid ,medicine.drug ,Cofilin 1 ,behavioral disciplines and activities ,Neuroprotection ,Temporal lobe ,03 medical and health sciences ,Seizures ,medicine ,Animals ,Cognitive Dysfunction ,Molecular Biology ,business.industry ,medicine.disease ,nervous system diseases ,Baicalein ,Rats ,Disease Models, Animal ,030104 developmental biology ,nervous system ,chemistry ,Epilepsy, Temporal Lobe ,Neurology (clinical) ,business ,Cognition Disorders ,030217 neurology & neurosurgery ,Oxidative stress ,Developmental Biology - Abstract
Temporal lobe epilepsy (TLE) is a chronic neurological disorder that is a refractory disease. Baicalein possesses various pharmacological activities, including neuroprotection in neurodegenerative disease. However, whether baicalein is protective in the treatment of TLE is not determined. Therefore, the present study investigated the role of baicalein in the treatment of TLE. Baicalein was injected intraperitoneally to TLE rats for two weeks after the onset of spontaneous recurrent seizures (SRS). Rats were observed for the occurrence of SRS, and cognitive and hippocampus injuries were evaluated. Oxidative stress and inflammatory cytokines were measured. Corticosterone and its receptor, actin-associated protein F-actin and cofilin-1 were investigated in the brains of epileptic rats. Baicalein significantly improved cognition and reduced hippocampus damage and mossy fibre sprouting in TLE rats without obvious SRS suppression. Baicalein produced excellent anti-oxidative and anti-inflammatory effects in TLE rats. Baicalein restored the disruption of the glucocorticoid signal pathway and actin-associated protein in TLE rats. These results suggest that the neuroprotective effects of baicalein on cognition and the hippocampus are associated with the suppression of oxidative stress and inflammation and the regulation of the glucocorticoid pathway and actin-associated protein in TLE rats. This evidence supports the use of baicalein as an adjuvant agent for epilepsy treatment.
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- 2018
13. Saussurea Lappa Modulates Gastrointestinal Motility, Motilin and Cholecystokinin Expression in Ulcer Rats
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Xiang-Chao Ling, Yu-Dong Wang, Teng-Yue Zhang, Yun-Xia Li, Qiankun Liang, Lan Fang Mao, Hong-Fang Li, Peng Wang, and Qing Wang
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0301 basic medicine ,medicine.medical_specialty ,Gastrointestinal tract ,Hepatology ,business.industry ,digestive, oral, and skin physiology ,Gastroenterology ,Motility ,Peptide secretion ,Motilin ,03 medical and health sciences ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Internal medicine ,medicine ,Duodenum ,business ,hormones, hormone substitutes, and hormone antagonists ,Omeprazole ,Migrating motor complex ,medicine.drug ,Cholecystokinin - Abstract
AIM : To investigate effects of Saussurea lappa (S.lappa) on rat chronic duodenal ulcer induced by ethylic acid and the underlying mechanisms. METHODS : Wistar rat chronic duodenal ulcer was patterned according to Okabe model while two pairs of bipolar stainless steel electrodes were implanted onto the serosal layer of gastrointestinal tract. Then, the rats were treated with water, Omeprazole, diverse-dose S.lappa for 10 days. Gastrointestinal motility, intestine propulsion and duodenal ulcer crater were detected. Expressions of cholecystokinin and motilin in duodenum were detected by immunohistochemistry (IHC). Motilin serum level was measured with enzyme-linked immunosorbent assay (ELISA). RESULT :Duodenal ulcer group rats had obvious disorders of gastrointestinal motility and brain-gut peptide secretion, including gastrointestinal myoelectric activity inhibition, migrating myoelectric complex (MMC) interruption, intestinal propulsion slowdown, cholecystokinin and motilin secretion decrease. Similarity to Omeprazole, S.lappa accelerated the coalescence of ethylic acid–induced duodenal ulcer significantly (p
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- 2016
14. Nitazoxanide protects cats from feline calicivirus infection and acts synergistically with mizoribine in vitro
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Jiang Shao, Yanli Zhao, Dongju Du, Hao Dong, Hailong Huang, Yinli Xie, Guohua Li, Yongkun Zhao, Yanbing Guo, Dengliang Li, Ying Zhang, Zhanding Cui, Kai Wang, Xiaoxueying Chen, Shuang Zhang, Hu Guixue, Qian Zhang, Fan Xingmeng, Shihui Zhao, and Teng Yue
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0301 basic medicine ,Nitazoxanide ,030106 microbiology ,Virulence ,Cat Diseases ,Antiviral Agents ,Article ,Cell Line ,03 medical and health sciences ,Virology ,Animals ,Medicine ,Feline calicivirus ,Antiviral ,Viral shedding ,Lung ,Pathogen ,Caliciviridae Infections ,Pharmacology ,CATS ,Mizoribine ,biology ,business.industry ,Drug Synergism ,Viral Load ,Nitro Compounds ,biology.organism_classification ,Virus Shedding ,Trachea ,Thiazoles ,in vivo ,030104 developmental biology ,Cats ,Female ,Ribonucleosides ,business ,Viral load ,Calicivirus, Feline ,medicine.drug - Abstract
Feline calicivirus (FCV) is a highly contagious pathogen that causes acute upper respiratory infections and oral disease in cats, thus seriously endangering feline health. Recently, there have been outbreaks of particularly virulent variant strains of FCV, which can cause both acute symptoms and fatal systemic disease. The discovery of effective antiviral agents to treat FCV infection is, therefore, gradually assuming increased importance. In this study, we showed that both nitazoxanide and mizoribine had antiviral activity in F81 cells infected with different strains of FCV and also demonstrated a synergistic effect between the two drugs. Experiments in cats challenged with FCV showed that nitazoxanide significantly reduced the clinical symptoms of FCV infection, reduced viral load in the trachea and lungs, and reduced viral shedding. Our results showed that nitazoxanide and mizoribine could potentially be used as therapeutic agents to treat FCV infection., Highlights • Mizoribine had antiviral activity against FCV. • Nitazoxanide and mizoribine had synergistic anti-FCV effects. • Nitazoxanide alleviated clinical symptoms in FCV-infected kittens. • Nitazoxanide significantly reduced viral titers in lungs and trachea of kittens infected with FCV.
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- 2020
15. Coronary Thermodilution Waveforms After Acute Reperfused ST‐Segment–Elevation Myocardial Infarction: Relation to Microvascular Obstruction and Prognosis
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Vannesa Teng Yue May, Ahmed Mahrous, David Corcoran, Keith G. Oldroyd, Mark C. Petrie, Mitchell Lindsay, Hany Eteiba, Stuart Watkins, Ian Ford, David Carrick, Stuart Hood, Jaclyn Carberry, Colin Berry, Nadeem Ahmed, Andrew Davie, Shu Ning Yew, Ify Mordi, and Margaret McEntegart
- Subjects
Male ,microvascular dysfunction ,Magnetic Resonance Imaging (MRI) ,Thermodilution ,coronary microvascular function ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,Coronary Angiography ,Ventricular Function, Left ,coronary microcirculation ,030218 nuclear medicine & medical imaging ,0302 clinical medicine ,magnetic resonance imaging ,Medicine ,ST segment ,Myocardial infarction ,pathophysiology ,Original Research ,Ventricular Remodeling ,medicine.diagnostic_test ,coronary microvascular resistance ,Heart ,Middle Aged ,Prognosis ,Coronary Vessels ,Interventional Cardiology ,Pathophysiology ,Hospitalization ,Editorial ,myocardial infarction ,medicine.anatomical_structure ,Risk stratification ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,Artery ,medicine.medical_specialty ,Magnetic Resonance Imaging, Cine ,Arterial Occlusive Diseases ,Diagnostic Testing ,Culprit ,magnetic resonance ,03 medical and health sciences ,Microvascular resistance ,Percutaneous Coronary Intervention ,Internal medicine ,Humans ,Mortality ,Anterior Wall Myocardial Infarction ,Aged ,Heart Failure ,business.industry ,Microcirculation ,Editorials ,Magnetic resonance imaging ,medicine.disease ,Microvessels ,ST Elevation Myocardial Infarction ,Vascular Resistance ,business - Abstract
Background Invasive measures of microvascular resistance in the culprit coronary artery have potential for risk stratification in acute ST‐segment–elevation myocardial infarction. We aimed to investigate the pathological and prognostic significance of coronary thermodilution waveforms using a diagnostic guidewire. Methods and Results Coronary thermodilution was measured at the end of percutaneous coronary intervention, (PCI) and contrast‐enhanced cardiac magnetic resonance imaging (MRI) was intended on day 2 and 6 months later to assess left ventricular (LV) function and pathology. All‐cause death or first heart failure hospitalization was a pre‐specified outcome (median follow‐up duration 1469 days). Thermodilution recordings underwent core laboratory assessment. A total of 278 patients with acute ST‐segment elevation myocardial infarction EMI (72% male, 59±11 years) had coronary thermodilution measurements classified as narrow unimodal (n=143 [51%]), wide unimodal (n=100 [36%]), or bimodal (n=35 [13%]). Microvascular obstruction and myocardial hemorrhage were associated with the thermodilution waveform pattern ( P =0.007 and 0.011, respectively), and both pathologies were more prevalent in patients with a bimodal morphology. On multivariate analysis with baseline characteristics, thermodilution waveform status was a multivariable associate of microvascular obstruction (odds ratio [95% confidence interval]=5.29 [1.73, 16.22];, P =0.004) and myocardial hemorrhage (3.45 [1.16, 10.26]; P =0.026), but the relationship was not significant when index of microvascular resistance (IMR) >40 or change in index of microvascular resistance (5 per unit) was included. However, a bimodal thermodilution waveform was independently associated with all‐cause death and hospitalization for heart failure (odds ratio [95% confidence interval]=2.70 [1.10, 6.63]; P =0.031), independent of index of microvascular resistance>40, ST‐segment resolution, and TIMI (Thrombolysis in Myocardial Infarction) Myocardial Perfusion Grade. Conclusions The thermodilution waveform in the culprit coronary artery is a biomarker of prognosis and may be useful for risk stratification immediately after reperfusion therapy.
- Published
- 2018
16. P6432Overlooked prognostic markers in NSTEMI: insights from the BHF FAMOUS-NSTEMI trial
- Author
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Colin Berry, Hany Eteiba, Jamie Layland, Stuart Watkins, Vannesa Teng Yue May, Thomas J. Ford, Mitchell Lindsay, Margaret McEntegart, Famous-Nstemi investigators, Alex McConnachie, Aadil Shaukat, Nick Curzen, Bethany Stanley, Keith G. Oldroyd, Mark C. Petrie, and Jaclyn Carberry
- Subjects
medicine.medical_specialty ,business.industry ,Internal medicine ,Medicine ,Cardiology and Cardiovascular Medicine ,business - Published
- 2018
17. Identification of Linear Tire Cornering Stiffness Using Subspace Methods
- Author
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Teng Yue Ba, Jianwu Zhang, and Xi Qiang Guan
- Subjects
Identification methods ,Engineering ,business.industry ,Subspace algorithms ,Stiffness ,Control engineering ,General Medicine ,Robustness (computer science) ,Control theory ,medicine ,medicine.symptom ,business ,Subspace topology ,Test data - Abstract
In this paper, subspace identification methods are proposed to estimate the linear tire cornering stiffness, which are only based on the road tests data without any prior knowledge. This kind of data-driven method has strong robustness. In order to validate the feasibility and effectiveness of the algorithms, a series of standard road tests are carried out. Comparing with different subspace algorithms used in road tests, it can be concluded that the front tire cornering stiffness can be estimated accurately by the N4SID and CCA methods when the double lane change test data are taken into analysis.
- Published
- 2014
18. Percutaneous Endoscopic Interlaminar Approach: Medial Foraminal Decompression in Treating Lumbar Disc Herniation or Spinal Stenosis
- Author
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Zhu Teng-Yue, Cui Hong-Peng, Ding Yu, Zhang Jian-Jun, Fu Ben-Sheng, and Qiao Jin-Lin
- Subjects
musculoskeletal diseases ,030222 orthopedics ,medicine.medical_specialty ,Percutaneous ,Spinal stenosis ,Decompression ,business.industry ,medicine.medical_treatment ,Lumbar spinal stenosis ,Cauda equina ,medicine.disease ,Surgery ,Lateral recess ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Lumbar ,Discectomy ,medicine ,business ,030217 neurology & neurosurgery - Abstract
Background: The technique of transforaminal endoscopic spine surgery is being widely used for lumbar degenerative diseases. But the interlaminar endoscopic surgery, which is more familiar and easier to be operated for spine surgeons, is more easily applied by traditionally trained surgeons. Objective: We propose the technique of percutaneous endoscopic medial foraminal decompression through interlaminar approach for the treatment of lumbar disc herniation (LDH) and spinal stenosis (LSS), and to explore the safety and efficacy of using this technique clinically. Methods: Thirty-two LDH and eleven LSS patients received medial foraminal decompression surgery with 22.6 ± 7.9 months follow-up. Through interlaminar space, we are able to perform discectomy and lateral recess decompression to decompress the medial foraminal area. Clinical efficacy was assessed by calculating the scores of VAS, SF-36, and lumbar disease JOA and ODI respectively at preoperative, postoperative and the discharge period, 3-6 months postoperatively and the final follow-up time point when patients were considered having received maximum surgical benefit. Follow-up time period varied because of the patients' follow-up logistics in China serving a large referral area made it difficult for rural patients to return at established intervals for the study. Results: For both LDH and LSS patients, the observational indexes of the follow-up time points showed significant differences compared with those preoperatively (P
- Published
- 2017
19. Chilling Injury Symptoms and Enzyme Activity Analysis of Lingwu Long Jujube
- Author
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Xi Hong Li, Teng Yue Wang, Lan Chen, and Xia Liu
- Subjects
Flesh ,fungi ,General Engineering ,food and beverages ,Outbreak ,Biology ,medicine.disease ,Polyphenol oxidase ,Enzyme assay ,Horticulture ,Metabolic pathway ,Fibrosis ,medicine ,biology.protein ,Chilling injury ,Peroxidase - Abstract
According to the observation to chilling injury symptoms of Lingwu Long Jujube, as well as the analysis to the enzyme activity related to the anti-adversity of plant in this paper, it is found that the disease will outbreak in 4-10 hours after low temperature pre-cooling, and the main symptoms include sinking semi-spherical dry spots and fibrosis of flesh. It is measured that the activity of polyphenol oxidase and phenylalnine ammonialyase in the diseased fruit is 0.34U and 101.689U separately, but the activity is 0.133U and 49.156U separately in healthy fruits, and there is a significant difference, but the difference of peroxidase is not significant. It laid the foundation for further research on relationship between chilling injury and metabolic pathway.
- Published
- 2014
20. Changes in epidemiological profiles of AIDS in China: a systematic analysis
- Author
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Bonnie Wong, Teng-Yue Hu, Rui Wang, Yu-Jie Zhang, Xiao-Chi Sun, and Xin-Zu Chen
- Subjects
medicine.medical_specialty ,business.industry ,Incidence (epidemiology) ,media_common.quotation_subject ,Public health ,General Medicine ,medicine.disease ,Acquired immunodeficiency syndrome (AIDS) ,Excellence ,Environmental health ,Epidemiology ,medicine ,Christian ministry ,Rural area ,China ,business ,media_common - Abstract
Background AIDS is a serious health burden globally. In China, despite great efforts, AIDS continues to be a substantial public health challenge. We aimed to provide a systematic analysis of changes in the epidemiology of AIDS by examining incidence and mortality of AIDS in China in the past decade. Methods We calculated crude incidence and mortality of AIDS in urban and rural areas in China from a publicly accessible Chinese Ministry of Health database between 1997 and 2017, specifically examining differences in mortality for men versus women (including children), rural versus urban, and differing age groups ( Findings The incidence of patients with AIDS gradually increased from 0·01 to 4·15 (ptrend Interpretation The burden of AIDS is increasing in China, with higher mortality among men, rural women, and youth. Understanding these epidemiological changes might allow for more focused prevention and treatment of AIDS in China. Funding The 1-3-5 Project for Disciplines of Excellence, West China Hospital, Sichuan University (ZY2017304).
- Published
- 2019
21. Early molecular responses of bone to obstructive nephropathy induced by unilateral ureteral obstruction in mice
- Author
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Yoseph Asmelash Gebru, Teng-Yue Diao, Hong-Wen Deng, Sa-Sa Gu, Yan Zhang, and Shu-Yan Wu
- Subjects
medicine.medical_specialty ,biology ,Bone Injury ,business.industry ,Osteoid ,H&E stain ,General Medicine ,urologic and male genital diseases ,medicine.disease ,Angiotensin II ,Bone resorption ,Obstructive Nephropathy ,Endocrinology ,Nephrology ,RANKL ,Internal medicine ,medicine ,biology.protein ,Renal osteodystrophy ,business - Abstract
Aim: This study was performed to address the bone injury and the early molecular responses of bone to obstructive nephropathy induced by unilateral ureteral obstruction in mice. Methods: The male mice were subjected to unilateral ureteral obstruction (UUO, n = 10) or sham operation (n = 10). All mice were killed on day 7 after the surgical operation. Hematoxylin and eosin and tartate-resistant acid phosphatase staining were performed on paraffin-embedded bone sections. Expression of genes and proteins was analyzed by reverse transcription-polymerase chain reaction, and Western blotting and immunohistochemistry staining, respectively. Results: The serum calcium level was significantly reduced in UUO mice compared with that of Sham mice. The proximal tibia of UUO mice exhibited the increased expansion of chondrocytes zone, the reduction of osteoid content, and the increased separation and disconnection of woven bones. Reverse transcription-polymerase chain reaction results showed the downregulation of Cbfa1 and Col mRNA expression and the upregulation of Tgf-β, CtsK, CaII, Opg and Rankl mRNA expression in tibia of UUO mice compared to those of Sham mice. The ratio of Opg and Rankl was unchanged between Sham and the UUO group. Local protein expression of angiotensin II and its type 2 receptor was dramatically upregulated in tibia of UUO mice. Conclusion: Together, it is concluded that the obstructive nephropathy has defective effects on bone, and the underlying mechanisms are the reduction of bone formation and the increase of bone resorption, which is mediated, at least partially through local angiotensin II signalling.
- Published
- 2012
22. Involvement of the Skeletal Renin-Angiotensin System in Age-Related Osteoporosis of Ageing Mice
- Author
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Rong Hai, Xiao-li Li, Shu-Yan Wu, Teng-Yue Diao, Sa-Sa Gu, Hong-Wen Deng, and Yan Zhang
- Subjects
Male ,Aging ,medicine.medical_specialty ,Bone density ,Osteoporosis ,Bone tissue ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,Renin-Angiotensin System ,Mice ,Bone Density ,Internal medicine ,Renin ,Renin–angiotensin system ,Animals ,Humans ,Medicine ,Femur ,Tibia ,Molecular Biology ,Bone mineral ,Mice, Inbred ICR ,Histocytochemistry ,business.industry ,Angiotensin II ,Organic Chemistry ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,Gene Expression Regulation ,Ageing ,Tomography, X-Ray Computed ,business ,Biotechnology - Abstract
The local tissue-specific renin-angiotensin system (RAS) was identified. The aim of this study was to investigate the role of local bone RAS in the osteoporosis of aging mice. Twelve-month-old and two-month-old male mice were respectively assigned to the ageing and young groups. The tibias and femurs were collected for an analysis of histomorphology, bone mass, and gene and protein expression. H&E staining and micro-CT measurement showed a loss of the trabecular bone network and decrease of bone mineral density in the proximal tibial metaphysis of the aged mice. The PCR results indicated the significant up-regulation of renin and angiotensinogen (AGT) mRNA expression in both the tibia and femur of the ageing mice. Western blotting data showed that the tibial angiotensin II protein expression was significantly increased in the ageing group. The enhancement of renin and AGT expression in the bone tissue resulted in the increased production of angiotensin II which plays an important role in the pathology of age-related osteoporosis.
- Published
- 2012
23. Acid-sensing ion channel 1a mediates acid-induced increases in intracellular calcium in rat articular chondrocytes
- Author
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Cheng-Wan Li, Yu Wang, Fei-Hu Chen, Wei Hu, Teng-Yue Zhang, Fan-rong Wu, Xia Li, Jian-Piing Li, Wei-Guo Lu, and Feng-Lai Yuan
- Subjects
Cartilage, Articular ,Male ,Time Factors ,Blotting, Western ,Clinical Biochemistry ,Fluorescent Antibody Technique ,Spider Venoms ,chemistry.chemical_element ,Nerve Tissue Proteins ,Calcium ,Sodium Channels ,Calcium in biology ,Rats, Sprague-Dawley ,Chondrocytes ,Microscopy, Electron, Transmission ,medicine ,Extracellular ,Animals ,RNA, Messenger ,Molecular Biology ,Cells, Cultured ,Acid-sensing ion channel ,Calcium metabolism ,Microscopy, Confocal ,L-Lactate Dehydrogenase ,Mechanosensation ,Reverse Transcriptase Polymerase Chain Reaction ,Chemistry ,Sodium channel ,Cartilage ,Cell Biology ,General Medicine ,Hydrogen-Ion Concentration ,Molecular biology ,Rats ,Acid Sensing Ion Channels ,medicine.anatomical_structure ,Peptides ,Sodium Channel Blockers - Abstract
Acid-sensing ion channels (ASICs) are cationic channels that are activated by extracellular acidification and implicated in pain perception, ischemic stroke, mechanosensation, learning, and memory. It has been shown that ASIC1a is an extracellular pH sensor in the central and peripheral nervous systems, but its physiological and pathological roles in non-neural cells are poorly understood. We demonstrated a novel physiological function of ASIC1a in rat articular chondrocytes. The expression of ASIC1a mRNA and protein in rat articular chondrocytes was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. The distribution of ASIC1a protein located in articular chondrocytes was determined by using immunofluorescence cell staining. The possible molecular mechanisms of articular chondrocytes pH sensing, as assessed by recording intracellular calcium ([Ca(2+)]i) in chondrocytes, were analyzed by using the laser scanning confocal microscopy technique. The cell injury following acid exposure was analyzed with lactate dehydrogenase release assay and electron microscopy. mRNA and protein expression showed that ASIC1a was expressed abundantly in these cells. In cultured chondrocytes, extracellular pH 6.0 increased intracellular calcium in the presence of extracellular Ca(2+). The ASIC1a-specific blocker PcTX venom significantly reduced this increase in [Ca(2+)]i, and inhibited acid-induced articular chondrocyte injury. However, the increase in [Ca(2+)]i and articular chondrocyte injury were not observed in the absence of extracellular Ca(2+). These findings show that increased [Ca(2+)]i, mediated via ASIC1a, might contribute to acidosis-induced articular chondrocyte injury.
- Published
- 2010
24. Assessment of Fractional Flow Reserve in Patients With Recent Non–ST-Segment–Elevation Myocardial Infarction
- Author
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Jamie Layland, Aleksandra Radjenovic, John D. McClure, Mark C. Petrie, David Carrick, Hany Eteiba, Arvind Sood, Mitchell Lindsay, Vannesa Teng Yue May, Anna O’Donnell, Matthew M.Y. Lee, Colin Berry, Stuart Watkins, Nadeem Ahmed, Keith G. Oldroyd, Margaret McEntegart, and Samuli M Rauhalammi
- Subjects
Male ,medicine.medical_specialty ,Acute coronary syndrome ,medicine.medical_treatment ,Myocardial Infarction ,Fractional flow reserve ,Coronary artery disease ,Electrocardiography ,Myocardial perfusion imaging ,Cardiac magnetic resonance imaging ,Internal medicine ,Myocardial Revascularization ,medicine ,Humans ,Myocardial infarction ,Aged ,Ejection fraction ,medicine.diagnostic_test ,business.industry ,Myocardial Perfusion Imaging ,Percutaneous coronary intervention ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Fractional Flow Reserve, Myocardial ,Treatment Outcome ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Background— The use of fractional flow reserve (FFR) in acute coronary syndromes is controversial. The British Heart Foundation Fractional Flow Reserve Versus Angiography in Guiding Management to Optimize Outcomes in Non-ST-Elevation Myocardial Infarction (FAMOUS-NSTEMI) study (NCT01764334) has recently demonstrated the safety and feasibility of FFR measurement in patients with non–ST-segment–elevation myocardial infarction. We report the findings of the cardiac magnetic resonance (CMR) substudy to assess the diagnostic accuracy of FFR compared with 3.0-T stress CMR perfusion. Methods and Results— One hundred six patients with non–ST-segment–elevation myocardial infarction who had been referred for early invasive management were included from 2 centers. FFR was measured in all major patent epicardial coronary arteries with a visual stenosis estimated at ≥30%, and if percutaneous coronary intervention was performed, an FFR assessment was repeated. Myocardial perfusion was assessed with stress perfusion CMR at 3 T. The mean age was 56.7±9.8 years; 82.6% were men. Mean time from FFR evaluation to CMR was 6.1±3.1 days. The mean±SD left ventricular ejection fraction was 58.2±9.1%. Mean infarct size was 5.4±7.1%, and mean troponin concentration was 5.2±9.2 μg/L. There were 34 fixed and 160 inducible perfusion defects. There was a negative correlation between the number of segments with a perfusion abnormality and FFR ( r =−0.77; P P Conclusions— FFR in patients with recent non–ST-segment–elevation myocardial infarction showed high concordance with myocardial perfusion in matched territories as revealed by 3.0-T stress perfusion CMR. Clinical Trial Registration— URL: http://www.clinicaltrials.gov . Unique identifier: NCT02073422.
- Published
- 2015
25. PROGNOSTIC SIGNIFICANCE OF CO-EXISTING PERSISTENT EDEMA AND HEMORRHAGE AT 6 MONTHS IN SURVIVORS OF ST-ELEVATION MYOCARDIAL INFARCTION
- Author
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Colin Berry, Ahmad Mahrous, Stuart Watkins, Ian Ford, Vannesa Teng Yue May, Jaclyn Carberry, Ify Mordi, Margaret McEntegart, Keith G. Oldroyd, Nadeem Ahmed, Stuart Hood, Andrew Davie, David Carrick, Mitchell Lindsay, Aleksandra Radjenovic, Caroline Haig, and Hany Eteiba
- Subjects
medicine.medical_specialty ,business.industry ,St elevation myocardial infarction ,Internal medicine ,Edema ,Cardiology ,medicine ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Published
- 2017
26. HYPERTENSION, MICROVASCULAR PATHOLOGY AND PROGNOSIS IN PATIENTS WITH AN ACUTE ST-SEGMENT ELEVATION MYOCARDIAL INFARCTION
- Author
-
Ahmed Mahrous, Stuart Watkins, Margaret McEntegart, Martin Lindsay, Andrew Davie, Ian Ford, Colin Berry, Jaclyn Carberry, Stuart Hood, Vannesa Teng Yue May, Hany Eteiba, Nadeem Ahmed, Ify Mordi, and David Carrick
- Subjects
medicine.medical_specialty ,business.industry ,Internal medicine ,Acute ST segment elevation myocardial infarction ,Cardiology ,Medicine ,In patient ,Cardiology and Cardiovascular Medicine ,business - Published
- 2017
27. CURRENT SMOKING, MICROVASCULAR PATHOLOGY AND ADVERSE OUTCOME AFTER ACUTE ST-SEGMENT ELEVATION MYOCARDIAL INFARCTION: NEW PATHOPHYSIOLOGICAL INSIGHTS
- Author
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Stuart Watkins, Ian Ford, Stuart Hood, Aleksandra Radjenovic, Hany Eteiba, Ahmed Mahrous, Naveed Sattar, Nadeem Ahmed, Jaclyn Carberry, David Carrick, Margaret McEntegart, Vannesa Teng Yue May, Ify Mord, Martin Lindsay, Colin Berry, Andrew Davie, Keith G. Oldroyd, Paul Welsh, Caroline Haig, and Mark C. Petrie
- Subjects
medicine.medical_specialty ,Pathology ,business.industry ,Adverse outcomes ,Acute ST segment elevation myocardial infarction ,medicine.disease ,Pathophysiology ,surgical procedures, operative ,Internal medicine ,Conventional PCI ,Cardiology ,Medicine ,Smoking status ,cardiovascular diseases ,Cardiology and Cardiovascular Medicine ,business ,Reperfusion injury ,Acute STEMI - Abstract
Background: The pathophysiological basis of reperfusion injury in smokers is incompletely understood. Methods: Patients with acute STEMI were prospectively enrolled during emergency PCI, and categorized according to a current smoking status (yes/no). Contrast-enhanced MRI at 1.5T was acquired 2
- Published
- 2017
28. Effects of angiotensin II type 1 receptor blocker on bones in mice with type 1 diabetes induced by streptozotocin
- Author
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Shu Yan Wu, Xi Chen, Teng Yue Diao, Man Sau Wong, Jing Yu Wang, Yan Zhang, Shu Ran, Yoseph Asmelash Gebru, and Sa Sa Gu
- Subjects
Blood Glucose ,Male ,medicine.medical_specialty ,Medicine (General) ,Osteoporosis ,Bone and Bones ,Streptozocin ,Diabetes Mellitus, Experimental ,Renin-Angiotensin System ,Endocrinology ,R5-920 ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,medicine ,Animals ,Femur ,RNA, Messenger ,Receptor ,Bone mineral ,Tibia ,business.industry ,Body Weight ,X-Ray Microtomography ,medicine.disease ,Streptozotocin ,Angiotensin II ,Osteopenia ,Losartan ,Diabetes Mellitus, Type 1 ,Mice, Inbred DBA ,business ,Angiotensin II Type 1 Receptor Blockers ,medicine.drug - Abstract
Introduction: This study was performed to address the pathological roles of the skeletal renin-angiotensin system (RAS) in type 1 diabetes-induced osteoporosis and the effects of the angiotensin II type 1 receptor blocker losartan on bones in diabetic mice. Materials and methods: Bone histomorphology was detected by H&E staining, Safranin O staining and X-ray radiography. Micro-CT was performed for the analysis of bone parameters. Gene and protein expression were determined by RT-PCR and immunoblotting. Results: Type 1 diabetic mice displayed osteopenia phenotype, and losartan treatment had no osteoprotective effects on diabetic mice as shown by the reduction of bone mineral density and microarchitectural parameters at the proximal metaphysis of the tibia. The mRNA expression of AGT, renin receptor and ACE, and protein expression of renin and AT1R were markedly up-regulated in the bones of vehicle-treated diabetic mice compared to those of non-diabetic mice. The treatment with losartan further significantly increased the expression of AGT, renin, angiotensin II and AT1R, and reduced the expression of AT2R receptor as compared to those of diabetic mice. Conclusion: Local bone RAS functionally played a role in the development of type 1 diabetic osteoporosis, and losartan had no bone-sparing function in diabetes mice because of enhance skeletal RAS activity.
- Published
- 2014
29. Protective effects of water fraction of Fructus Ligustri Lucidi extract against hypercalciuria and trabecular bone deterioration in experimentally type 1 diabetic mice
- Author
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Teng Yue Diao, Chun-Tao Che, Man Sau Wong, Liang Wang, and Yan Zhang
- Subjects
Male ,medicine.medical_specialty ,Hypercalciuria ,Ligustrum ,chemistry.chemical_element ,Parathyroid hormone ,Calcium ,Diabetes Mellitus, Experimental ,Mice ,Diabetes mellitus ,Internal medicine ,Drug Discovery ,medicine ,Animals ,DNA Primers ,Pharmacology ,Kidney ,Base Sequence ,Plant Extracts ,Reverse Transcriptase Polymerase Chain Reaction ,X-Ray Microtomography ,medicine.disease ,Streptozotocin ,Urinary calcium ,Fibroblast Growth Factor-23 ,medicine.anatomical_structure ,Endocrinology ,Diabetes Mellitus, Type 1 ,chemistry ,Mice, Inbred DBA ,Osteoporosis ,Calcium-sensing receptor ,medicine.drug - Abstract
Ethnopharmacological relevance Fructus Ligustri Lucidi (FLL), the fruit of Ligustrum lucidum Ait, is a commonly prescribed herb to nourish the endocrine and renal systems and to strengthen the bones in Traditional Chinese Medicine. This study was aimed to determine the effects of water fraction of FLL ethanol extract (WF-EE) on urinary calcium excretion and trabecular bone properties in type 1 diabetic mice. Materials and methods The DBA/2J mice with type 1 diabetes induced by streptozotocin injection were orally administered with WF-EE. After 6 weeks of treatment, the level of biomarkers, including serum calcium, parathyroid hormone (PTH), and fibroblast growth factor-23 (FGF-23) and urine calcium, was measured. Micro-CT was performed to detect trabecular bone properties of the proximal tibial metaphysis. The expression of active calcium transporting proteins in kidney and duodenum was determined by RT-PCR, immunoblotting and immunostaining. Results Type 1 diabetes induced hypercalciuria and trabecular bone deterioration. The WF-EE could significantly inhibit hypercalciuria and ameliorate the micro-structure of trabecular bone as well as increase serum PTH and FGF-23 levels in type 1 diabetic mice. The gene expressions of active calcium transporting proteins in duodenum were up-regulated, and the gene and protein expressions of calcium-sensing receptor (CaSR) in kidney were dramatically down-regulated in diabetic mice in response to the treatment with WF-EE. Conclusions The present study demonstrated the protective effects of the water fraction of Fructus Ligustri Lucidi ethanol extract against hypercalciuria and trabecular bone deterioration in experimentally type 1 diabetic mice, and the underlying mechanism may be attributed to its regulations on duodenal calcium transporting proteins and renal CaSR.
- Published
- 2014
30. Discoidin domain receptor 2 facilitates prostate cancer bone metastasis via regulating parathyroid hormone-related protein
- Author
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Teng Yue, Hou Huilian, Zhang Yan, Li Xu, Su Jin, Li Jing, Zhang Wei, Yao Libo, Li Juan, and Yin Zhan-hai
- Subjects
Male ,Osteoclasts ,Electrophoretic Mobility Shift Assay ,Receptor tyrosine kinase ,Prostate cancer ,Mice ,Runx2 ,Cell Movement ,Transforming Growth Factor beta ,Tumor Cells, Cultured ,DDR2 ,Phosphorylation ,RNA, Small Interfering ,Luciferases ,Promoter Regions, Genetic ,biology ,Chemistry ,Reverse Transcriptase Polymerase Chain Reaction ,Bone metastasis ,Osteoblast ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,Molecular Medicine ,Signal Transduction ,medicine.medical_specialty ,PTHrP ,Blotting, Western ,Bone Neoplasms ,Real-Time Polymerase Chain Reaction ,Bone resorption ,Collagen Type I ,Osteoclast ,Internal medicine ,medicine ,Cell Adhesion ,Animals ,Humans ,RNA, Messenger ,Molecular Biology ,Discoidin Domain Receptors ,Cell Proliferation ,Wound Healing ,Osteoblasts ,Parathyroid hormone-related protein ,Parathyroid Hormone-Related Protein ,Prostatic Neoplasms ,Receptor Protein-Tyrosine Kinases ,medicine.disease ,Endocrinology ,Receptors, Mitogen ,biology.protein ,Cancer research ,Discoidin domain - Abstract
Prostate cancer frequently metastasizes to the skeleton but the underlying mechanism remains largely undefined. Discoidin domain receptor 2 (DDR2) is a member of receptor tyrosine kinase (RTK) family and is activated by collagen binding. This study aimed to investigate the function and detailed mechanism of DDR2 in prostate cancer bone dissemination. Herein we found that DDR2 was strongly expressed in bone-metastatic prostate cancer cells and tissues compared to that in normal controls. Enhanced expression of constitutively activated DDR2 led to elevation in motility and invasiveness of prostate cancer cells, whereas knockdown of DDR2 through specific shRNA caused a dramatic repression. Knockdown of DDR2 in prostate cancer cells resulted in significant decrease in the proliferation, differentiation and function of osteoblast. Over-expression of DDR2 in prostate cancer cells resulted in notable acceleration of osteoclast differentiation and bone resorption, whereas knockdown of DDR2 exhibited the opposite effects. An intrabone injection bone metastasis animal model demonstrated that DDR2 promoted osteolytic metastasis in vivo. Molecular evidence demonstrated that DDR2 regulated the expression, secretion, and promoter activity of parathyroid hormone-related protein (PTHrP), via modulating Runx2 phosphorylation and transactivity. DDR2 was responsive to TGF-β and involved in TGF-β-mediated osteoclast activation and bone resorption. In addition, DDR2 facilitated prostate cancer cells adhere to type I collagen. This study reveals for the first time that DDR2 plays an essential role in prostate cancer bone metastasis. The mechanism disclosure may provide therapeutic targets for the treatment of prostate cancer.
- Published
- 2013
31. Effects of angiotensin-converting enzyme inhibitor, captopril, on bone of mice with streptozotocin-induced type 1 diabetes
- Author
-
Hai Pan, Fang-Yi Zhang, Xi Chen, Teng-Yue Diao, Yan Zhang, Man Sau Wong, and Sa-Sa Gu
- Subjects
Male ,medicine.medical_specialty ,Captopril ,Endocrinology, Diabetes and Metabolism ,Acid Phosphatase ,Osteocalcin ,Osteoclasts ,Angiotensin-Converting Enzyme Inhibitors ,Bone and Bones ,Streptozocin ,Diabetes Mellitus, Experimental ,Renin-Angiotensin System ,Mice ,Random Allocation ,Endocrinology ,Osteoprotegerin ,Bone Density ,Transforming Growth Factor beta ,Internal medicine ,Renin ,medicine ,Animals ,Orthopedics and Sports Medicine ,RNA, Messenger ,Tartrate-resistant acid phosphatase ,Bone mineral ,biology ,Tibia ,Chemistry ,Tartrate-Resistant Acid Phosphatase ,Angiotensin II ,Acid phosphatase ,NF-kappa B ,Angiotensin-converting enzyme ,General Medicine ,Up-Regulation ,Isoenzymes ,Bone Diseases, Metabolic ,Diabetes Mellitus, Type 1 ,Mice, Inbred DBA ,biology.protein ,medicine.drug - Abstract
There are contradictory results about the effect of angiotensin-converting enzyme inhibitors (ACEIs) on bone. This study was performed to address the skeletal renin-angiotensin system (RAS) activity and the effects of the ACEI, captopril, on the bone of streptozotocin-induced type 1 diabetic mice. Histochemical assessment on bone paraffin sections was conducted by Safranin O staining and tartrate-resistant acid phosphatase staining. Micro-computed tomography was performed to analyze bone biological parameters. Gene and protein expression were determined by real-time polymerase chain reaction and immunoblotting, respectively. Type 1 diabetic mice displayed osteopenia phenotype and captopril treatment showed no osteoprotective effects in diabetic mice as shown by the reduction of bone mineral density, trabecular thickness and bone volume/total volume. The mRNA expression of ACE and renin receptor, and the protein expression of renin and angiotensin II were markedly up-regulated in the bone of vehicle-treated diabetic mice compared to those of non-diabetic mice, and these molecular changes of skeletal RAS components were effectively inhibited by treatment with captopril. However, treatment with captopril significantly elevated serum tartrate-resistant acid phosphatase 5b levels, reduced the ratio of osteoprotegerin/receptor activator of nuclear factor-κB ligand expression, increased carbonic anhydrase II mRNA expression and the number of matured osteoclasts and decreased transforming growth factor-β and osteocalcin mRNA expression in the tibia compared to those of diabetic mice. The present study demonstrated that the use of the ACEI, captopril, has no beneficial effect on the skeletal biological properties of diabetic mice. However, this could be attributed, at least partially, to its suppression of osteogenesis and stimulation of osteoclastogenesis, even though it could effectively inhibit high activity of local RAS in the bone of diabetic mice.
- Published
- 2013
32. Potential of RAS Inhibition to Improve Metabolic Bone Disorders
- Author
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Emmanuel Mukwaya, Yan Zhang, Hai Pan, Teng-Yue Diao, and Yoseph Asmelash Gebru
- Subjects
medicine.medical_specialty ,lcsh:Medicine ,Angiotensin-Converting Enzyme Inhibitors ,Review Article ,Pharmacology ,Biology ,Bone tissue ,Models, Biological ,Bone and Bones ,General Biochemistry, Genetics and Molecular Biology ,Bone remodeling ,Renin-Angiotensin System ,Angiotensin Receptor Antagonists ,Internal medicine ,Renin–angiotensin system ,medicine ,Humans ,Hormone metabolism ,General Immunology and Microbiology ,lcsh:R ,General Medicine ,Angiotensin II ,Metabolic Bone Disorder ,Bone Diseases, Metabolic ,Endocrinology ,medicine.anatomical_structure ,Signal transduction ,Signal Transduction - Abstract
Metabolic bone disorder is usually caused by abnormalities of minerals and hormones metabolism. Recently, it has been proved by several studies that the renin-angiotensin system (RAS) in local bone tissue is directly involved in bone metabolism. Activation of skeletal RAS plays an important role in bone metabolic disorders. Based onin vitro,in vivo, and clinical studies, this review explains the roles of RAS in bone metabolism and also covers the potential approaches and beneficial effects of RAS inhibition on bone health. Differential strategies for inhibiting RAS can be employed to maintain bone health, which are attributed primarily to the reduced level of angiotensin II (AngII) and suppressed stimulation of the AngII signaling pathway. The use of renin inhibitors, angiotensin-converting enzyme inhibitors, and AngII receptor blockers either individually or in combination with each other could have promising results in fighting bone metabolic disorders associated with other cardiovascular diseases as well as independent bone injuries.
- Published
- 2013
33. Trabecular bone deterioration at the greater trochanter of mice with unilateral obstructive nephropathy
- Author
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Xi Chen, Man Sau Wong, Yoseph Asmelash Gebru, Yan Zhang, Sa-Sa Gu, and Teng-Yue Diao
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Greater trochanter ,X-ray microtomography ,Urology ,Short Communication ,urologic and male genital diseases ,Mice ,Imaging, Three-Dimensional ,Medicine ,Animals ,Femur ,Renal osteodystrophy ,Pathological ,Bone mineral ,Chronic Kidney Disease-Mineral and Bone Disorder ,Mice, Inbred ICR ,Trochanter ,business.industry ,urogenital system ,General Medicine ,X-Ray Microtomography ,medicine.disease ,Obstructive Nephropathy ,female genital diseases and pregnancy complications ,Disease Models, Animal ,sense organs ,Bone Diseases ,business ,Ureteral Obstruction - Abstract
Our previous study showed the early molecular responses of bone in response to obstructive nephropathy in a unilateral ureteral obstruction (UUO) mouse model. Here, we addressed the changes in trabecular bone properties at greater trochanter, the proximal and the distal metaphysis of femur in UUO mice. The male mice were subjected to UUO (n=10) or sham operation (n=10). All mice were killed on day 7 after the surgical operation. The micro-computed tomography (micro-CT) analysis for different femoral trabecular bone sites demonstrated pathological alterations of trabecular bone mass and micro-networks at greater trochanter as shown by decreases in bone mineral density/bone volume (P
- Published
- 2012
34. Blockade of acid-sensing ion channels protects articular chondrocytes from acid-induced apoptotic injury
- Author
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Chao Rong, Mei-Ying Lin, Fan-rong Wu, Jie Tang, Teng-Yue Zhang, Fei-Hu Chen, Cheng-Cheng Zhang, Feng-Lai Yuan, Wei Hu, and Sheng Jiang
- Subjects
Epithelial sodium channel ,Male ,Cell Survival ,Immunology ,Caspase 3 ,Apoptosis ,Nerve Tissue Proteins ,Sodium Channels ,Amiloride ,Rats, Sprague-Dawley ,Chondrocytes ,medicine ,Animals ,Ion channel ,Acid-sensing ion channel ,Cells, Cultured ,Pharmacology ,biology ,Chemistry ,Calpain ,Sodium channel ,Calcineurin ,Anatomy ,Cell biology ,Rats ,Acid Sensing Ion Channels ,biology.protein ,Calcium ,Hydrochloric Acid ,medicine.drug ,Sodium Channel Blockers - Abstract
Acid-sensing ion channels (ASICs) are members of the degenerin/epithelial sodium channel (DEG/ENaC) protein superfamily and play a critical role in acid-induced cell injury. In this study, we examined whether drugs such as amiloride that block ASICs could attenuate acid-induced apoptotic injury to articular chondrocytes.Articular chondrocytes were isolated from Sprague-Dawley rats, and their phenotype was determined by toluidine blue and immunocytochemical staining. Articular chondrocyte viability assay was performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). Apoptosis of chondrocytes was observed by the terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling method as well as propidium iodide labeling methods. Intracellular calcium ([Ca(2+)](i)) was analyzed by a Ca(2+)-imaging method. In addition, the expression levels of calpain and calcineurin in articular chondrocytes were examined by real-time PCR and immunocytochemical staining. The activity of caspase-3 was evaluated by spectrophotometric assays.Positive staining for glycosaminoglycan and collagen II was seen in articular chondrocytes. Blocking acid-sensing ion channels significantly decreased the cell death percentage and increased cell viability following acid exposure. After pretreated with amiloride, acid-induced [Ca(2+)](i) rises were reduced. Amiloride also inhibited calpain and calcineurin expression levels in acid-induced chondrocytes, and inhibited caspase-3 activity.The data presented in this study provided some experimental evidence that blocking ASICs could protect acid-induced apoptotic injury to chondrocytes.
- Published
- 2011
35. Inhibition of acid-sensing ion channels in articular chondrocytes by amiloride attenuates articular cartilage destruction in rats with adjuvant arthritis
- Author
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Wei Hu, Xia Li, Rui-Sheng Xu, Feng-Lai Yuan, Teng-Yue Zhang, Fan-rong Wu, Fei-Hu Chen, Wei-Guo Lu, Jian-Ping Li, and Cheng-Wan Li
- Subjects
musculoskeletal diseases ,Cartilage, Articular ,Male ,medicine.medical_specialty ,Immunology ,Type II collagen ,Arthritis ,Nerve Tissue Proteins ,Calcium in biology ,Sodium Channels ,Amiloride ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Chondrocytes ,Internal medicine ,Lactate dehydrogenase ,medicine ,Extracellular ,Animals ,Aggrecans ,Collagen Type II ,Aggrecan ,Acid-sensing ion channel ,Cells, Cultured ,Pharmacology ,Dose-Response Relationship, Drug ,Chemistry ,musculoskeletal system ,medicine.disease ,Arthritis, Experimental ,Rats ,Acid Sensing Ion Channels ,Endocrinology ,medicine.drug ,Sodium Channel Blockers - Abstract
The aim of this study was to examine whether drugs such as amiloride that block acid sensing ion channels (ASICs) could attenuate articular cartilage destruction in adjuvant-induced arthritis (AA).Articular chondrocytes were isolated from the normal rats, and intracellular calcium ([Ca(2+)]i) was analyzed with laser scanning confocal microscopy. The cell injury was analyzed with lactate dehydrogenase release assay and electron microscopy. Amiloride or phosphate buffered saline was administered daily to AA rats for 1 week from the time of arthritis onset. Morphology of the articular cartilage was examined by hematoxylin and eosin staining, and Mankin score was calculated. The expression level of type II collagen (COII) and aggrecan mRNA and proteins in the articular cartilage was evaluated by real-time PCR and Western blotting, respectively.The rapid decrease in extracellular pH (6.0) induced a conspicuous increase in [Ca(2+)]i in the articular chondrocytes. Amiloride reduced this increase in [Ca(2+)]i, and inhibited acid-induced articular chondrocyte injury. Amiloride significantly decreased Mankin scores in the articular cartilage in AA rats. COII and aggrecan mRNA and protein expression in the articular cartilage was significantly increased by amiloride.These findings represent some experimental evidence of a potential role for ASICs in the pathogenesis of articular cartilage destruction in rheumatoid arthritis.
- Published
- 2010
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