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Abstract Background Regulatory applications for cell therapy face more objections compared to conventional small molecule or biological drugs, leading to delays in market approval and clinical adoption. Increased regulatory objections frequently relate to issues regarding preclinical evidence, such as experimental design of animal studies, selection of animal models, endpoints, and determination of mechanism of action. Synthesis and clarification of the preclinical evidence necessary to demonstrate treatment efficacy and advance into early-phase clinical trials is needed to help researchers avoid regulatory objections. Methods We conducted a scoping review in which we searched repositories of the International Council for Harmonisation and all national member organizations (N = 38) for documents related to preclinical studies of cell therapies. Active guidance documents related to cell therapy were included, with no restrictions based on the year or language of publication. Data extraction was conducted in duplicate with conflicts resolved through consensus discussion. Results From 1215 identified documents, a total of 182 were included and analyzed, with 71% originating from ten major regulatory agencies. The most prevalent preclinical item addressed was the mechanism of action (n = 161, 88% of documents), underscoring its importance in bridging preclinical findings to clinical application. Most documents (n = 140, 77%) emphasized the importance of using clinically relevant preclinical models, though specific recommendations on models of disease were less common (n = 81, 45%). Selection of clinically relevant intervention parameters (n = 136, 75%) and outcome measures (n = 121, 66%) were also frequently recommended, but selection of relevant comparator groups appeared less frequently (n = 35, 19%). Furthermore, robust study design elements such as randomization and blinding were less frequently recommended, appearing in 31% of documents (n = 57). Comparison with clinical trial guidance revealed a significant gap in the rigor of study design recommendations for preclinical research. Conclusions Regulatory guidance for preclinical efficacy studies often recommends a strong emphasis on the clinical relevance of animal models, intervention parameters, outcomes, and mechanism of action. Incorporating these recommendations into early preclinical studies should improve the acceptability of preclinical evidence for approval by the relevant national regulators and can be used as a guide to ensure that all evidence that regulators say they expect is efficiently assembled into new clinical trial applications.

Abstract Okra (Abelmoschus esculentus L. Moench) is a vital vegetable crop known for its nutritional and economic significance, especially in tropical and subtropical regions. Studying heterosis and combining ability in okra is crucial for enhancing its yield, quality, and resistance to pests and diseases. Heterosis can lead to superior offspring with enhanced traits while understanding combining ability helps in identifying the best parent combinations for breeding programs. Okra is an often cross-pollinated crop; therefore, exploiting heterosis is advantageous. The study was conducted from 2021 to 2022 at the Experimental Farm and Quality Analysis Laboratory, Department of Vegetable Science, College of Horticulture, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India to evaluate the heterosis and combining ability in okra to facilitate the development of high-yielding, resilient cultivars. The experimental material consisted of an F1 population of 30 crosses obtained from 10 parental lines crossed with three testers in a Line × Tester mating design, plus a standard check (Punjab-8). Estimates of heterosis (heterobeltiosis and standard heterosis) of the cross combinations UHFO-6 × Pusa Bhindi-5, UHFO-6 × Arka Anamika and UHFO-9 × Arka Anamika were high for inter-nodal distance, number of pods per plant, average pod weight, pod yield per plant, harvest duration, hundred seed weight, mucilage content, etc. Higher estimates of general combining ability (GCA) effects for pod yield per plant were observed in the parental lines UHFO-6 (123.47) and UHFO-9 (7.49). Among the cross combinations, UHFO-10 × Hisar Unnat (38.81), UHFO-2 × Pusa Bhindi-5 (38.29), UHFO-2 × Arka Anamika (17.42), and UHFO-5 × Arka Anamika (15.06) demonstrated higher estimates of specific combining ability (SCA) effects for pod yield per plant. The cross UHFO-2 × Hisar Unnat (160.00) exhibited the highest heterobeltiosis for mucilage content, while UHFO-10 × Arka Anamika (562.03) showed the highest standard heterosis for total polyphenol content. These cross combinations could produce okra with enhanced nutritional and medicinal properties. The highest GCA and SCA effects for pod yield per plant were observed in UHFO-6 (123.47) and UHFO-10 × Hisar Unnat (38.81), respectively. Identifying these parental lines and cross combinations based on their combining ability can result in the development of okra hybrids with substantially higher yields. In future, after multi-location trials, these parents and crosses can be released to replace existing okra cultivars (hybrids/varieties). Higher yielding and better quality okra cultivars can enhance the profitability for farmers, contribute to food security, and meet market demands more efficiently.

Abstract The presence of refractory micropollutants in natural waters poses significant environmental and health risks. Preferably, advanced oxidation techniques like electro-Fenton (EF) and bio-electro-Fenton (BEF) are used to mitigate micropollutants; nevertheless, their field-scale implementation is limited by prohibitive catalyst cost. As an alternative, waste-iron electrocoagulated algal biomass (A-BC/Fe) was explored as a heterogeneous Fenton catalyst to eliminate dimethyl phthalate (DMP) from wastewater. The Fenton-conducive morphological, chemical, and electrochemical properties of the A-BC/Fe catalyst were revealed by detailed characterisation. In EF treatment, 10 mg/L of DMP was completely degraded within 15 min at pH of 7.0, 50 mM Na2SO4, and cathode potential of − 1.4 V vs. Ag/AgCl. Moreover, the EF system achieved 87.80 ± 2.10% and 96.14 ± 1.10% of DMP removal from secondary and tertiary treated municipal sewage, respectively. The A-BC/Fe catalyst-driven EF process disintegrated DMP into benign non-toxic by-products and showed stable performance over eight batch cycles with only a 1.71% decline in DMP removal efficiency. Further, the A-BC/Fe-catalysed BEF system eliminated 94.81 ± 1.90% of DMP in 4 h while achieving a maximum power density of 124.03 ± 5.64 mW/m2. This investigation underscores the potential of repurposing electrocoagulated algal biomass as a sustainable heterogenous catalyst for micropollutant remediation.

Abstract Energy harvesters based on nanomaterials are getting more and more popular, but on their way to commercial availability, some crucial issues still need to be solved. The objective of the study is to select an appropriate nanomaterial. Using features of the Reinforcement Deep Q-Network (DQN) in conjunction with Fuzzy PROMETHEE, the proposed model, we present in this work a hybrid fuzzy approach to selecting appropriate materials for a vehicle-environmental-hazardous substance (EHS) combination that operates in roadways and under traffic conditions. The DQN is able to accumulate useful experience of operating in a dynamic traffic environment, accordingly selecting materials that deliver the highest energy output but at the same time bring consideration to factors such as durability, cost, and environmental impact. Fuzzy PROMETHEE allows the participation of human experts during the decision-making process, going beyond the quantitative data typically learned by DQN through the inclusion of qualitative preferences. Instead, this hybrid method unites the strength of individual approaches, as a result providing highly resistant and adjustable material selection to real EHS. The result of the study pointed out materials that can give high energy efficiency with reference to years of service, price, and environmental effects. The proposed model provides 95% accuracy with a computational efficiency of 300 s, and the application of hypothesis and practical testing on the chosen materials showed the high efficiency of the selected materials to harvest energy under fluctuating traffic conditions and proved the concept of a hybrid approach in True Vehicle Environmental High-risk Substance scenarios.

Abstract The usage of mobile robots (MRs) has expanded dramatically in the last several years across a wide range of industries, including manufacturing, surveillance, healthcare, and warehouse automation. To ensure the efficient and safe operation of these MRs, it is crucial to design effective control strategies that can adapt to changing environments. In this paper, we propose a new technique for controlling MRs using reinforcement learning (RL). Our approach involves mathematical model generation and later training a neural network (NN) to learn a policy for robot control using RL. The policy is learned through trial and error, where MR explores the environment and receives rewards based on its actions. The rewards are designed to encourage the robot to move towards its goal while avoiding obstacles. In this work, a deep Q-learning (QL) agent is used to enable robots to autonomously learn to avoid collisions with obstacles and enhance navigation abilities in an unknown environment. When operating MR independently within an unfamiliar area, a RL model is used to identify the targeted location, and the Deep Q-Network (DQN) is used to navigate to the goal location. We evaluate our approach using a simulation using the Epsilon-Greedy algorithm. The results show that our approach outperforms traditional MR control strategies in terms of both efficiency and safety.

Abstract Mental health issues have increased substantially since the onset of the COVID-19 pandemic. However, health policymakers do not have adequate data and tools to predict mental health demand, especially amid a crisis. Using time-series data collected in Singapore, this study examines if and how algorithmically measured emotion indicators from Twitter posts can help forecast emergency mental health needs. We measured the mental health needs during 549 days from 1 July 2020 to 31 December 2021 using the public’s daily visits to the emergency room of the country’s largest psychiatric hospital and the number of users with “crisis” state assessed through a government-initiated online mental health self-help portal. Pairwise Granger-causality tests covering lag length from 1 day to 5 days indicated that forecast models using Twitter joy, anger and sadness emotions as predictors perform significantly better than baseline models using past mental health needs data alone (e.g., Joy Intensity on IMH Visits, χ2 = 14·9, P < ·001***; Sadness Count on Mindline Crisis, χ2 = 4·6, P = ·031*, with a one-day lag length). The findings highlight the potential of new early indicators for tracking emerging public mental health needs.

Abstract Firecrackers are a vital element of cultural festivities that happen worldwide. However, the hazardous by-products they emit have a significant impact on environmental pollution, leading to the greenhouse effect and climate change. Aluminium powder serves as the fuel in the traditional flash powder mixture, nitrate of potassium serves as an oxidizing agent, and sulphur acts as the igniter at exact concentrations. The presence of sulfur in the flash powder mixture is critical as it acts as an igniter, contributing to the formation of sulfur dioxide, which can cause environmental harm. We carried out an experiment employing Sargassum wightii brown seaweed powder as a replacement for sulphur at specific amounts to lessen the effects of sulphur in flash powder. We discovered that Sargassum wightii brown seaweed powder may replace up to 50% of the sulphur significance in the flash powder mixture without impairing the flash powder's traditional performance. Our experiments included impact and friction sensitivity, SEM, and FTIR analyses to evaluate the improved flash powder composition. The results revealed that the modified flash powder mixture SP5 and SP10 emits less emission by 12% and 21%, and produces similar noise performance of 108 and 107 dB(A) to the normal flash powder composition (SP), affirming that the SP10 flash powder is a viable alternative. Moreover, in our relentless pursuit to mitigate the detrimental effects on our environment, we have ingeniously introduced a novel product—the Chinese cracker made from vegetable waste paper. Not only does this innovative solution address concerns regarding land pollution, but it also presents a sustainable approach to consumer goods.

Abstract Preimplantation embryos can experience stress from laboratory interventions and a sub-optimal culture environment. Though research has demonstrated advanced maternal age impairs oocyte quality, the response of embryos derived from such oocytes to vitrification-thawing and culture in a high oxygen (O2) environment in the assisted reproductive technology laboratory is unknown. Therefore, in this study, embryos produced by in vitro fertilization (IVF) using oocytes from two- and eight-month-old Swiss albino mice were vitrified and thawed during their 6–8 cell stage. and cultured at low oxygen (5%) tension (LOT) and high oxygen (20%) tension (HOT). Embryo development, apoptosis, inner cell mass (ICM) outgrowth proliferation ability in vitro and pluripotency were assessed. Embryos from advanced maternal age cultured at HOT showed reduced fertilizing ability (p

Purpose: The primary goal of this study is to evaluate the effect of the non-invasive radiofrequency hyperthermia (RFHT) device on chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) rat model and investigate the underlying mechanism. Materials and Methods: In this study, Sprague-Dawley rats were randomly distributed into three groups: (1) normal control group, (2) CP/CPPS group, and (3) RFHT group. CP/CPPS rat models were induced by 17β-estradiol and dihydrotestosterone for 4 weeks and RFHT was administered for 5 weeks after model establishment. During RFHT administration, core body temperatures were continuously monitored with a rectal probe. After administering RFHT, we assessed pain index for all groups and collected prostate tissues for Western blot analysis, immunofluorescence, and immunohistochemistry. We also collected adjacent organs to the prostate including urinary bladder, testes, and rectum for safety assessment via H&E staining along with a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay. Results: After administering RFHT, pain in rats was significantly alleviated compared to the CP/CPPS group. RFHT reduced high-mobility group box 1 (HMGB1) expression and improved inflammation by downregulating subsequent proinflammatory cytokines through inhibition of the toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway. In prostate-adjacent organs, no significant histological alteration or inflammatory infiltration was detected. The area of cell death also did not increase significantly after RFHT. Conclusions: In conclusion, RFHT demonstrated anti-inflammatory effects by inhibiting the HMGB1-TLR4-NF-κB pathway in CP/CPPS rat models. This suggests that RFHT could serve as a safe and promising therapeutic strategy for CP/CPPS.

Objective: Mastalgia, a common complaint among women, denotes breast discomfort that can manifest as cyclical or non-cyclical. Reassurance, mechanical support and various non-pharmacological treatments, like flaxseeds, have been seen to have a good effect in treating mastalgia. Thus, the aim of this study was to investigates the efficacy of flaxseed in alleviating pain associated with mastalgia and its impact on the overall health-related quality of life among female patients. Materials and Methods: Conducted at a tertiary care center in Northern India over 18 months, it employed a single-arm interventional design. The participants included females aged 18 years and older presenting with breast pain at the Department of General Surgery. The intervention involved daily consumption of 30 g of milled flaxseed for each participant, administered over a period of six months. Pain severity was assessed using the visual analogue scale (VAS) before supplementation and at follow-up intervals up to six months. Concurrently, the Short Form-12 (SF-12) items Health Survey measured health-related quality of life, encompassing both physical and mental health domains. Statistical analysis employed parametric (paired t-test) and non-parametric tests (chi-square, McNemar) where appropriate, with statistical significance set at p

Background: Yellow phosphorus-containing rodenticide poisoning as a means of deliberate self-harm is being widely encountered in South India. Hence, a comprehensive study delineating its profile and patient outcome is required to implement measures to control the persistent threat. MATERIALS AND METHODS: This is a single-center, retrospective observational study of all patients presenting to the emergency department with a history of consumption of yellow phosphorus rodenticide poisoning between 2014 and 2020. Institutional review board approval was obtained before commencement. Results: During the study period, 164 patients who presented with consumption of yellow phosphorous were included in the analysis. The mean age with standard deviation of patients was 27.5 (10.6) years, with a female preponderance (87/164: 53.1%). N-acetyl cysteine (NAC) was administered in 87.2% (143/164), and its administration within 48 h of poison consumption showed a significant correlation with good outcomes (72.3% vs. 50%; OR: 0.38; 95% CI: 0.18–0.80; P-value = 0.012). Altered sensorium, hypoxemia, transaminitis, hyperbilirubinemia, and hypoalbuminemia had significant correlations with bad outcomes. The in-hospital mortality rate was 9.7% (16/164), and 21.4% (35/164) of the patients left against medical advice. Conclusion: Yellow phosphorous poisoning is associated with significant mortality. NAC given within 48 h of patient presentation had a significant correlation with good outcomes.

Background: In the intricate landscape of healthcare amid the COVID-19 pandemic, the emergence of mucormycosis as a severe complication posed a significant challenge to primary healthcare. This study delved into the complexities of adverse drug reactions (ADRs) in COVID-19 patients with mucormycosis undergoing treatment with conventional amphotericin B. Methods: Ethically approved and meticulously conducted, the study scrutinized 154 ADRs in depth, shedding light on their classification, outcomes, and interventions in COVID patients with mucormycosis. A descriptive analysis was carried out to report the findings of this study. Results: The findings revealed that a substantial proportion (85.6%) of these ADRs were manageable, emphasizing the need for vigilant monitoring and timely interventions. Notably, gender disparities surfaced, indicating potential gender-specific responses to amphotericin B. Causality assessments based on the WHO-UMC scale classified the majority of ADRs as certain, providing a robust foundation for understanding the intricate relationships between amphotericin B and the observed adverse events. Conclusion: This research not only categorizes ADRs as preventable and manageable but also offers practical insights into their nature and the diverse strategies employed for their management. The study’s outcomes underline the importance of personalized healthcare approaches that can be adopted by primary care physicians for effective patient care.

Context: The prevalence of gestational diabetes mellitus (GDM) in the Indian subcontinent has increased exponentially, especially after new diagnostic criteria. Identifying women who need close monitoring is a challenge. Ultrasound has been used to guide therapy but its use in the management of GDM has been used indiscriminately due to paucity of information. The use of ultrasound to guide the number of antenatal visits for adequate glycemic control has not been researched adequately. There is also a need to contrive antenatal care strategies that can obtain optimal neonatal outcomes without burdening the health system in low-middle income countries (LMIC). AIMS: Our study was to compare pragmatic standard management of GDM with an ultrasound-guided regime (USGR) to prevent neonatal complications to ensure optimal care. Settings and Designs: A randomized controlled trial was done in a tertiary hospital between May 2019 and September 2021 in South India. Methods and Materials: Five hundred and eighty-eight women were randomized to a standard regime or USGR. Statistical Analysis: χ2 and t-test were used to compare outcomes. Results: Maternal and neonatal outcomes between standard and ultrasound USGR showed no significant difference in outcomes. Fetuses in USGR with AC ≥ 70th centile had macrosomia (3.59% vs 26.67%, P < 0.001), increased severity of diabetes (2.97% vs 16.67%, P < 0.001), 3rd- and 4th-degree perineal tear (2.95% vs 27.27%, P < 0.001). The increased number of visits did not decrease adverse outcomes. Conclusion: This small well-designed study did not show any advantage with USGR as compared to the pragmatic simple standard regime, in the treatment of women with GDM.

Trimethylaminuria (TMAU), colloquially known as fish odour syndrome, is a rare metabolic disorder characterised by the body’s inability to properly metabolise Trimethylamine (TMA), resulting in a distinctive and unpleasant fishy odour in bodily secretions. This comprehensive review explores the pathophysiology, clinical manifestations, genetic basis, diagnostic challenges, and psychological impact of TMAU, along with management strategies. Genetic mutations affecting the flavin-containing monooxygenase 3 (FMO3) enzyme, which is responsible for TMA metabolism, contribute to the condition’s hereditary nature. Diagnosis can be challenging due to intermittent symptoms and overlaps with other conditions, such as Olfactory Reference Syndrome (ORS). The psychosocial impact of TMAU can be profound, leading to embarrassment, social isolation, and mental health issues. Management strategies include dietary modifications, supplements, antibiotics, and hygiene measures to alleviate symptoms. Despite advances in understanding TMAU, further research is needed to enhance diagnostic accuracy and therapeutic approaches, ultimately improving the quality of life for affected individuals.

Introduction: Obesity is a prevalent co-morbidity in Polycystic Ovarian Syndrome (PCOS), causing metabolic disturbances that lead to changes in endocrinological homeostasis. Adipokines are proinflammatory molecules secreted by adipose tissues that affect metabolic and endocrinological mechanisms. Excess androgen secretion alters fat distribution and deposition, leading to increased visceral obesity in PCOS, which is a major concern for the development of cardiometabolic events. Aim: To assess the Visceral Adiposity Index (VAI) and levels of different adipokines in non obese and obese PCOS patients, and to establish the relationship between VAI and circulating adipokines in both non obese and obese phenotypes of PCOS patients. Materials and Methods: This cross-sectional study was conducted at the Mayo Institute of Medical Sciences, Barabanki, Uttar Pradesh, India from November 2019 to August 2021. A total of 144 patients diagnosed with PCOS according to the Rotterdam criteria were divided into non obese and obese categories based on Body Mass Index (BMI). VAI was calculated using a sex-specific formula, and serum levels of adiponectin, resistin, and leptin were measured by Enzyme Linked Immuno Sorbent Assay (ELISA). Fasting glucose, insulin, and serum lipid profiles were evaluated. Data were statistically analysed using Student’s t-tests and Pearson’s correlation coefficient. Weighted linear regression was used to detect any relationships between adipokines, obesity indicators, and VAI. Results: A total of 144 female patients with a mean age of 27.47±0.33 years suffering from PCOS were included in this study. VAI was significantly higher (p-value=0.034) in non obese patients than in obese patients. There was a significant correlation between VAI and resistin (r-value=0.302, p-value=0.011) and leptin (r-value=0.308, p-value=0.009) in the non obese PCOS group. Additionally, there was a significant association between obesity indicators {BMI, Body Fat Percentage (BFP), Waist Circumference (WC), and Hip Circumference (HC)} and VAI; this association was absent in the obese subgroup. According to the regression model, leptin was the only statistically relevant predictor for VAI among the studied PCOS patients (t-value=2.278, p-value=0.024). Conclusion: VAI was higher in non obese PCOS patients. An imbalance in adipokines (leptin and resistin) may lead to the accumulation of visceral fat without significantly affecting BMI.

Dendrobium ovatum is a tropical epiphytic orchid endemic to the Western Ghats of India and has been listed as a threatened species in recent research due to its declining populations and changes in flowering and fruit set patterns. This study aims to investigate the mycoflora associated with the roots, stems and leaves of D. ovatum. Both surface‐associated and endophytic fungal associates were isolated and identified using morphological and molecular methods. The study resulted in the isolation of 139 cultures, which were divided into 24 morphotypes, 99% of which belonged to Ascomycota. The most dominant members, Trichoderma harzianum and Colletotrichum gloeosporioides, were consistently observed across all the study sites. Tissue‐specific fungal diversity analysis revealed that each organ was dominated by a distinct fungal group, forming characteristic communities specific to each tissue. The roots of D. ovatum exhibited the highest species richness and diversity, compared to the stem and leaves. This research also represents the first documentation of fungal associates of the threatened orchid D. ovatum.

Abstract We use a 16-month-long, 20 Hz wind data from a mooring deployed in the Bay of Bengal (BoB) to study the characteristics of turbulent wind stress ( $${u}{\prime}{w}{\prime})$$ u ′ w ′ ) events in the marine atmospheric boundary layer (MABL). Quadrant analysis of the motion-corrected $${u}{\prime}$$ u ′ and $${w}{\prime}$$ w ′ suggests that sweep and ejections, representing downward stress transfer into the ocean, dominate the $${u}{\prime}{w}{\prime}$$ u ′ w ′ (~ 140%). In comparison, outward and inward interactions representing an upward stress transfer into the atmosphere provide the counter-contribution (~ 40%). We found a wind speed (ws) dependency on stress transfer for ws > 3 m/s, while for low ws, the swell-dominated ocean state modulates the $${u}{\prime}{w}{\prime}$$ u ′ w ′ with a significant reverse stress transfer into the atmosphere, especially during intermonsoon periods. It is found that for weak winds ( $$ws$$ ws

Abstract Droughts and floods are examples of extreme weather events that can result from changes in ocean temperature. Ocean temperature is a key component of the global open sea system. Currently, real-time sea surface temperature (SST) forecasts are generated by numerical models based on physics principles and influenced by boundary and initial conditions. These models generally perform better over large areas than at specific locations. To address this and improve prediction accuracy, particularly in high-precision areas, the Coati Optimization Algorithm-based Deep Convolutional Forest (COA-DCF) method is proposed. This optimization approach is utilized to train the Deep Convolutional Forest (DCF) classifier, which then applies the prediction strategy. The COA-DCF method forecasts ocean surface temperature anomalies by considering key variables such as SST, Sea Surface Height (SSH), soil moisture, and wind speed, using historical data ranging from 1 to 10 days across six different locations. The proposed method achieves improved accuracy with low Root Mean Square Error (RMSE) and Mean Absolute Error (MAE) values, and a high Pearson’s correlation coefficient (r) of 0.493, 0.487, and 0.4733, respectively, thereby enhancing the overall performance of the deep learning model.

Introduction: Sleep disturbances are highly prevalent among children with Autism Spectrum Disorder (ASD). Many studies on sleep in children with ASD have reported a high incidence of irregular sleep patterns and poor sleep quality. Recent research has highlighted the potential link between Sensory Processing (SP) difficulties and sleep disturbances in children with ASD. Therefore, a comprehensive knowledge of the evidence exploring the relationship between SP and sleep in children with ASD is needed to provide insights into targeted interventions and support strategies to improve sleep outcomes and the overall quality of life in children with ASD. Aim: This systematic review aims to investigate the relationship between SP and sleep in children with ASD aged 3 to 12 years. Materials and Methods: A comprehensive search of articles was conducted across databases, including Scopus, PubMed, and OTseeker. The following major keywords were used during the database searches: Sensory processing, sensory integration, sensory processing disorder, sensory integration disorder, sleep, sleep disorder, sleep problems, sleep disturbances, and autism spectrum disorder. The McMaster Critical Review Form for Quantitative Studies was utilised to evaluate the methodological quality of the included articles. Results: Through electronic and manual searches, 2,612 articles were identified after removing duplicates. Among these, nine articles published between January 2012 and December 2022 met the predetermined inclusion criteria. Of these, eight studies reported a statistically significant correlation between SP difficulties and sleep disturbances. Conclusion: This review underscores the significant impact of SP challenges on the sleep quality of children diagnosed with ASD, aged 3 to 12 years.

Introduction: The estimation of serum bioelements for the identification of oral premalignant and malignant lesions has been extensively studied. Higher amounts of copper have been found in all smoking and Smokeless Tobacco (SLT) products, which are responsible for the higher incidence of premalignant and malignant lesions in the oral cavity. Limited studies have shown the significance and role of salivary bioelements in premalignant lesions, such as Oral Submucous Fibrosis (OSMF) and oral leukoplakia, and malignant lesions like Squamous Cell Carcinoma (SCC) of the oral cavity. Aim: To estimate salivary copper levels in premalignant and malignant lesions of the oral cavity. Materials and Methods: The preent cross-sectional study was undertaken in Tagore Dental College and Hospital in Chennai, Tamil Nadu, India for a period of 6 months, from March 2022 to August 2022. A total of 50 patients were recruited and divided into five groups of 10 patients each: Group I- control group; Group II- SLT chewing habit without any oral lesion; Group III- 10 patients with Tobacco Pouch Keratosis (TPK); Group IV- 10 patients with OSMF; and Group V- 10 patients with oral SCC. About 5 mL of salivary samples were collected and centrifuged at 3000 rpm to obtain the supernatant saliva for analysis. The levels of copper were estimated using photo calorimetry. Statistical analysis was done using One-way Analysis of Variance (ANOVA) followed by a Post-hoc test. Results: A total of 50 patients with a mean age of 40±20 years were included in present study, comprising 46% females and 54% males. There was a statistically significant difference (p

Background: Millions of children worldwide suffer from chronic asthma, a serious respiratory disease that has a major influence on everyday living and health outcomes. There are significant public health concerns regarding the estimated 10-15% frequency of asthma in children in India. Vitamin D, which is recognized for its immunomodulatory qualities, according to recent studies reduces the severity. In this study, children aged 6 to 12 years old's vitamin D levels and the severity of their asthma were examined. Materials and Methods: At a tertiary care teaching hospital, 100 children between the ages of 6 and 12 who were presenting with acute exacerbations of bronchial asthma were the subjects of a prospective cohort study from August 2021 to August 2022. ELISA was used to evaluate serum 25-hydroxyvitamin D levels, and the GINA recommendations were used to determine the severity of asthma. Based on their levels of vitamin D, the kids were divided into three groups: sufficient (>30 ng/mL), insufficient (20–30 ng/mL), and deficient (

Abstract Sea level rise (SLR) poses a significant threat to coastal regions worldwide, particularly affecting over 60 million people living below 10 m above sea level along the African coast. This study analyzes the spatio-temporal trends of sea level anomaly (SLA) and its components (thermosteric, halosteric and ocean mass) in the Eastern Tropical Atlantic Ocean (ETAO) from 1993 to 2022. The SLA trend for the ETAO, derived from satellite altimetry, is 3.52 ± 0.47 mm/year, similar to the global average of 3.56 ± 0.67 mm/year. Of the three upwelling regions, the Gulf of Guinea (GoG) shows the highest regional trend of 3.42 ± 0.12 mm/year. Using the ARMORD3D dataset, a positive thermosteric sea level trend of 0.88 ± 0.04 mm/year is observed, particularly in the equatorial and southern Atlantic regions. The steric component drives the interannual SLA variability, while the ocean mass component dominates the long-term trends, as confirmed by the GRACE and GRACE-FO missions for 2002–2022. For those two decades, the total SLR from altimetry amounts to 3.80 ± 0.8 mm/year, whilst the steric component is reduced to only 0.19 ± 0.05 mm/year, leaving a residual increase in the ETAO of 3.69 ± 0.5 mm/year. The independent mass change from GRACE amounts to 2.78 ± 0.6 mm/year for this region, which just closes the sea level budget within present uncertainty levels. Spatial analysis of the steric components indicates a warming along the equatorial African coast including the GoG and a freshening near Angola. Strong correlations with regional climate factors, particularly the Tropical South Atlantic Index, highlight the influence of persistent climate modes. These findings underscore the urgent need for mitigation and adaptation strategies to SLR in the ETAO, especially for densely populated coastal communities.

Leiomyomas are benign smooth muscle tumors most commonly seen in the uterus occasionally in the gastrointestinal tract. Pulmonary localization is extremely rare with the incidence of

The frequency of Anterior Cruciate Ligament Reconstructions (ACLR) has risen in recent years and is projected to continue increasing. Knee-joint infections consequent to this procedure, although rare, present a significant concern due to their potential severity. Hence, comprehending the progression of this complication and its detrimental effects if not promptly addressed is crucial. The impact of infection on the joint can vary depending on the duration between onset, diagnosis, and initiation of appropriate treatment. In some cases, the infection may have minimal consequences for the joint, while in others, it could lead to compromised viability of the Anterior Cruciate Ligament (ACL) graft, damage to the articular cartilage, or even premature onset of osteoarthritis. In the present case, a 42-year-old male had presented to the Orthopaedics Outpatient Department (OPD) six weeks postoperatively following an ACL reconstruction with clinical and laboratory findings pointing towards a septic knee. Lab findings suggested a raised ESR-71 mm/hr, CRP-127 mg/dL, and TLC-10,200/microL. The decision for immediate arthroscopic debridement with synovectomy was taken to achieve decompression all the while preserving the graft. This was followed by antibiotic saline irrigation for two days with strict antibiotic coverage following it.

Scoliosis is characterised by lateral spinal curvature, twisting of the spine and chest, and abnormalities in the sagittal profile. Physical therapy plays a crucial role in preventing the progression of scoliosis and its associated deformities, and in improving outcomes and quality of life. However, specific treatment methods for correcting the curvature and enhancing function are not extensively documented. Therefore, the present case report incorporated several techniques. A 17-year-old male visited the Physiotherapy Department with complaints of continuous pain in his lower back for the past 45 days. Due to the pain, the patient was not able to play football and sleep properly. Additionally, there was a notable postural deviation with a history of more than six years. His parents ignored his walking as stylish. Moreover, the patient stated that he had continuous pain, was not able to bend the spine backward, concentrate on studies and attend social gatherings. Upon spinal examination, scoliosis with a dropped right shoulder was observed. The patient was effectively treated using Myofascial Release (MFR) techniques combined with exercises to improve chest mobility. After management, the pain score showed a reduction, and there was improvement in the disability questionnaire. Significant changes were also noted in X-ray assessments and Range of Motion (ROM).

Introduction: Overactive bladder (OAB) syndrome is a chronic disease characterized by urinary urgency with or without urge incontinence, frequency, and nocturia and antimuscarinic drugs such as solifenacin have been the mainstay of treatment. Mirabegron a beta 3 adrenoreceptor agonist has recently gained importance in the management of OAB. The rationale of the study is that mirabegron improves the storage function without affecting voiding which increases the therapeutic effectiveness. The objective was to determine the therapeutic effectiveness of mirabegron versus solifenacin. Methods: A prospective observational study was conducted on 298 patients with OAB syndrome attending the urology outpatient department of government medical college after obtaining institutional review board clearance. Patients of both genders, belonging to the 18–65 years of age group, attending the urology outpatient department were selected for the study. Patients were evaluated using the OAB-validated 8-question awareness tool (OAB-V8 score) before and after receiving drugs by direct questionnaire method after receiving informed consent. Patients were prescribed either solifenacin 5 mg or mirabegron 25 mg once daily by the urologist. Follow-up was done after 4 and 12 weeks. Adverse drug reactions of the drugs were assessed using the Central Drug Standard Control Organization suspected adverse reaction (ADR) form, and ADRs were notified to the nearest ADR monitoring center. Results: The mirabegron group showed maximum improvement in the mean OAB-V8 score values from baseline at 4 weeks (12.82 ± 5.86, P < 0.001) and 12 weeks (5.74 ± 3.31, P < 0.001) when compared to solifenacin. OAB-V8 scores of the solifenacin group also showed significant improvement from the baseline at 4 weeks (15.30 ± 5.54, P < 0.001) and 12 weeks (8.05 ± 4.59, P < 0.001). Heart rate, systolic, and diastolic blood pressures did not show significant changes during the follow-up in both the study groups. Thirteen patients developed ADRs such as dry mouth (four patients) and constipation (nine patients) in the solifenacin group. No ADRs were noted in the mirabegron group. Conclusion: Mirabegron showed maximum improvement in the OAB-V8 scores in patients with OAB syndrome, although the solifenacin group also showed improvement. Adverse effects were less in the mirabegron group when compared to the solifenacin group.

Skeletal class III malocclusion is a therapeutic challenge in orthodontic practice. Reverse functional appliances such as reverse twin block (RTB) are used to correct class III skeletal and occlusal disharmonies associated with functional anterior shift in growing patients. However, treatment options become limited with increasing age, especially when patients desire nonsurgical and nonextraction camouflage treatment. This report illustrates the successful nonsurgical treatment of class III malocclusion during adolescence and adulthood, exacerbated by a functional anterior shift that resulted in overclosure of the mandible. A modified fixed RTB was utilized to posture the mandible backwards, thereby inducing active clockwise rotation of the mandible. After treatment, patients demonstrated significantly improved maxillomandibular relationships, well-maintained stable occlusion, and facial esthetics. Satisfactory occlusal, esthetic, and functional outcomes achieved in the present cases underline the fact that dentoalveolar changes induced by fixed RTB can be utilized even past a patient’s peak pubertal growth period to obtain changes that aid in correcting a class III malocclusion. A synergistic combination of modified fixed RTB therapy accompanied by comprehensive fixed mechanotherapy is a viable treatment alternative for the correction of aptly selected mild to moderate skeletal class III malocclusions associated with functional anterior shift, anterior crossbites, and mandibular overclosure.

Introduction: Spasticity is a pervasive and debilitating condition. Anti-spastic medications like Baclofen and Diazepam are most commonly used for spasticity management. Objectives: The study aimed to evaluate and compare the efficacy of Baclofen with that of Diazepam in the management of spasticity following acquired spinal cord injuries. Methods: In this randomised open-label study involving 60 patients, 30 patients received 5 mg of oral Baclofen thrice daily and 30 patients received 5 mg of oral Diazepam thrice daily for the management of spasticity. The extent of spasticity was measured by Modified Ashworth Scale (MAS). The efficacy of these drugs was measured with the mean improvement in the above parameters on days 7, 14, 28, 56 and 90 from the start of pharmacotherapy. Results: Baseline muscle spasticity scores were comparable. At the endpoint, mean improvement in tone of right and left hip flexors (1.43 vs. 0.87), hip adductors (1.30 vs. 0.87), knee flexors (0.80 vs. 0.37), ankle plantar flexors (0.80 vs. 0.37) and ankle dorsiflexors (1.23 vs. 0.93) were significantly higher in Baclofen group (p

Introduction: Gestational Diabetes Mellitus (GDM) affects maternal placental blood flow. As a compensatory mechanism for placental haemodynamic changes, blood flow is redistributed from peripheral vessels to the brain. This re-distribution can be evaluated using doppler Ultrasonography (USG) measurements of the umbilical arteries and foetal middle cerebral arteries. Aim: To compare doppler flow parameters {Pulsatility Index (PI), Resistance Index (RI), Peak Systolic Velocity (PSV), Systolic-To-Diastolic (S/D)} of the foetal Middle Cerebral Artery (MCA) in pregnancy with gestational diabetes versus normal pregnancy, and to compare pregnancy outcomes in GDM versus non-GDM women. Materials and Methods: A prospective cohort study was conducted from November 2019 to November 2021 at the Department of Obstetrics and Gynaecology, Department of Radiology, and Department of Paediatrics in Tertiary care centre of New Delhi, India. All pregnant women attending the Antenatal Care Outpatient Department (ANC OPD) upto 34 weeks with an Oral Glucose Tolerance Test (OGTT) ≥140 mg/dL were defined as cases, and those with OGTT

Abstract The present investigation reported that FYM application in different seasons influenced root, shoot, and seedling length, straw K, vigour index-I, nutrient uptake, grain, and stover yield of pearl millet significantly (P kharif > rabi. Applying FYM in both seasons resulted in higher N, P, and K content in pearl millet grain (1.99%, 0.17%, and 0.37%, respectively) followed by kharif season application (1.93, 0.16, and 0.35%, respectively). Applying 15 t FYM ha−1 significantly increased the grain N (13.19%), P (63.16%), K (22.29%), protein (13.56%), stover N (32.76%), P (46.66%) and root length (29.83%) over FYM0. After 50 cropping cycles, continuous application of FYM15, FYM10, and FYM5 significantly improved vigour index-I by 52.85, 39.26, and 23.63% over no FYM, respectively. Applying 120 kg N ha−1 significantly increased N (6.38%), P (15.89%), and protein (6.03%) content, germination (5.91%), and vigour indexes (24.52 to 30.91%) of pearl millet grain over no fertilizer N. The treatment FYM15 × N120 increased the seedling length of pearl millet by 30.54 over N120 and 11.08% over FYM15 alone, respectively. Adding FYM either during both seasons or in the kharif season along with fertilizer N proved superior in improving the quality and yield of pearl millet.

Abstract Smart elevators provide substantial promise for time and energy management applications by utilizing cutting edge artificial intelligence and image processing technology. In order to improve operating efficiency, this project designs an elevator system that uses the YOLO model for object detection. Compared to traditional methods, our results show a 15% improvement in wait times and a 20% reduction in energy use. Due to the elevator’s increased accuracy and dependability, users’ qualitative feedback shows a high degree of pleasure. These results imply that intelligent elevator systems can make a significant contribution to more intelligent building management. Due to the elevator’s increased accuracy and dependability, users’ qualitative feedback shows a high degree of pleasure. These results imply that intelligent elevator systems can make a significant contribution to more intelligent building management. The successful integration of artificial intelligence (AI) and image processing technologies in elevator systems presents a promising foundation for future research and development. Further advancements in object detection algorithms, such as refining YOLO models for even higher accuracy and real-time adaptability, hold potential to enhance operational efficiency. Integrating smart elevators more deeply into IoT networks and building management systems could enable comprehensive energy management strategies and real-time decision-making. Predictive maintenance models tailored to elevator components could minimize downtime and optimize service schedules, enhancing overall reliability. Additionally, exploring adaptive user interfaces and personalized scheduling algorithms could further elevate user satisfaction by tailoring elevator interactions to individual preferences. Sustainable practices, including energy-efficient designs and integration of renewable energy sources, represent crucial avenues for reducing environmental impact. Addressing security concerns through advanced encryption and access control mechanisms will be essential for safeguarding sensitive data in smart elevator systems.

Abstract The transformer mineral oil is generally hydrocarbon-based and is not environmentally friendly, so it holds a significant share in environmental pollution. As an alternative to conventional transformer oil, plant-based insulation oil has been investigated globally in the past few decades. Even though vegetable oils are considered an environmentally viable alternative to mineral oil, the extensive utilization of vegetable oils could create a threat to Indian food security. The present work focuses on using Pongamia Pinnata oil (PPO) as a significant alternative to conventional transformer oil (CTO) in distribution transformers. The effectiveness of the proposed PPO is experimentally verified using thermal studies and electrical studies through a low-level distribution transformer of 1 kVA. A comparative analysis was carried out between the proposed and conventional oil regarding physical, chemical, and thermal properties. Also, scanning electron microscopy (SEM) and Energy Dispersive X-ray spectroscopy analysis have been carried out for fibreglass cloth insulation material with PPO and CTO to ensure the intrinsic structural strength of the insulation material. The structural strength ensures the bonding and life span of the insulation in the transformer. The cost–benefit analysis is also favourable for the proposed oil as a better green liquid dielectric for transformers.

Background: Military troops deployed in high altitude areas (HAAs) may suffer from illnesses peculiar to the terrain which are usually due to hypoxia. The physiological dynamics at high altitude (HA) are different from those in the plains and hence environmental factors play an important role at HA. The effect, if any, of hemodynamic changes in terms of raised hemoglobin (Hb) levels in HA on otological disorders is not very well researched and needs to be explored further. This study was undertaken to study the clinical manifestations, if any, of the hemodynamic alterations in the inner ear and their correlation with the Hb levels after 1 year deployment at HA. Methods: A longitudinal descriptive study was conducted over 1 year duration at a Military Hospital located at HAA on 99 soldiers who were

Abstract In many emerging nations, rapid industrialization and urbanization have led to heightened levels of air pollution. This sudden rise in air pollution, which affects global sustainability and human health, has become a significant concern for citizens and governments. While most current methods for predicting air quality rely on shallow models and often yield unsatisfactory results, our study explores a deep architectural model for forecasting air quality. We employ a sophisticated deep learning structure to develop an advanced system for ambient air quality prediction. We utilize three publicly available databases and real-world data to obtain accurate air quality measurements. These four datasets undergo a data cleaning to yield a consolidated, cleaned dataset. Subsequently, the Fused Eurasian Oystercatcher-Pathfinder Algorithm (FEO-PFA)—a dual optimization method combining the Eurasian Oystercatcher Optimizer (EOO) and Pathfinder Algorithm (PFA)—is applied. This method aids in selecting weighted features, optimizing weights, and choosing the most relevant attributes for optimal results. These optimal features are then incorporated into the Multiscale Depth-wise Separable Adaptive Temporal Convolutional Network (MDS-ATCN) for the ambient Air Quality Prediction (AQP) process. The variables within MDS-ATCN are further refined using the proposed FEO-PFA to enhance predictive accuracy. An empirical analysis is performed to compare the efficacy of our proposed model with traditional methods, underscoring the superior effectiveness of our approach. The average cost function is reduced to 5.5%, the MAE to 28%, and the RMSE to 14% by the suggested method, according to the performance research conducted with regard to all datasets.

Abstract Objective Proteasomes are conserved proteases crucial for proteostasis in eukaryotes and are promising drug targets for protozoan parasites. Yet, the proteasomes of Entamoeba histolytica remain understudied. The study’s objective was to analyse the differences in the substrate binding pockets of amoeba proteasomes from those of host, and computational modelling of β5 catalytic subunit, with the goal of finding selective inhibitors. Results Comparative sequence analysis revealed differences in substrate binding sites of E. histolytica proteasomes, especially in the S1 and S3 pockets of the catalytic beta subunits, implying differences in substrate preference and susceptibility to inhibitors from host proteasomes. This was strongly supported by significantly lower sensitivity to MG132 mediated inhibition of amoebic proteasome β5 subunit’s chymotryptic activity compared to human proteasomes, also reflected in lower sensitivity of E. histolytica to MG132 for inhibition of proliferation. Computational models of β4 and β5 subunits, and a docked β4-β5 model revealed a binding pocket between β4-β5, similar to that of Leishmania tarentolae. Selective inhibitors for visceral leishmaniasis, LXE408 and compound 8, docked well to this pocket. This functional and sequence-based analysis predicts differences between amoebic and host proteasomes that can be utilized to develop rationally designed, selective inhibitors against E. histolytica.

Background: Snakebites are a common medical emergency and occupational hazard for children in India, particularly in rural areas where poverty is prevalent. However, there is limited data on the epidemiology of snakebites on the Indian subcontinent. Objective: This cross-sectional, observational study aims to investigate the epidemiology, major clinical manifestations, and outcomes of snakebites in children under the age of 15 who were admitted to a tertiary care center in Bihar, a state in East India, and draw attention to this public health concern. Methods: A cross-sectional observational study was conducted at the Department of Paediatrics, Patna Medical College and Hospital, Patna. The study included all cases of snakebites with features of envenomation involving patients less than 15 years of age who were brought to the department over a 2-year period. Data were collected using a data collection form and analyzed using the Statistical Package for the Social Sciences, version 11.0 (SPSS Inc., Chicago, IL, USA). Results: A total of 59 cases were recorded, with 62.71% (n = 37) being male and 37.28% (n = 22) being female. Kraits were responsible for 38.9% (n = 23) of cases, vipers for 42.3% (n = 25), and cobras for 5% (n = 3). Fang marks were present in 67.7% (n = 40) of cases, and the majority of bites (84.7%, n = 50) occurred on a lower limb during the day. The age distribution showed that 16.9% (n = 10) were below 5 years old, 44% (n = 26) were between 5 and 10 years old, and 22% (n = 13) were above 10 years old. Traditional treatment was used in 44.7% (n = 22) of cases, with the most common treatments being local incision + tourniquet (22%, n = 13) and no traditional treatment (55.9%, n = 33). The highest number of cases occurred during July–September (35.5%, n = 21). Conclusion: Snakebites are a significant public health issue in Bihar, India, with the majority of cases occurring in rural areas. The study highlights the importance of increased awareness and preparedness among healthcare providers and the general public, particularly during the monsoon season. Early hospital transfer, prehospital management, and prevention should be promoted through regular public health initiatives.

Introduction: Gastrointestinal (GI) parasites are major health concerns in both humans and domestic animals. Livestock farming is one of the common livelihood practices in rural Nepal. The proximity at human and domestic animal interface increases the chances of dissemination of enteric parasites, especially those of zoonotic importance. This study was aimed at finding the parasite prevalence and risk factors in both humans and their domestic animals in Jirel community. Materials and Methods: A field survey was conducted on the Jirel ethnic people and their domestic animals in Dolakha district, where a total of 152 fresh fecal samples from humans and domestic animals (cow, pigs, goats, chickens, ducks, and pigeons) were collected. The feces were examined by wet mounts and concentration techniques. A structured questionnaire survey was carried out among the local people and owners of the domestic animals to gather sociodemographic information, awareness, and hygienic practices in relation to parasite transmission Results: The enteric parasite prevalence was found to be highest in goats (80.0%;12/15), followed by pigs (55.55%;5/9), cows (45.45%;6/11), chickens (11.7%;4/34), and humans (1.41%;1/71), while the fecal samples of ducks and pigeons did not contain any parasites. The only parasite identified in humans was Ascaris lumbricoides. Similarly, three genera of GI parasites (Eimeria sp., Strongyloides sp, and Trichuris sp.) from goats, two genera each from cow (Eimeria sp. and Strongyloides sp.), pigs (Entamoeba sp. and A. suum), and chickens (Eimeria sp. and Ascaridia galli), were detected Conclusions: Based on the direct field observation, questionnaire survey and laboratory analysis, it is concluded that the Jirel community people are aware of health and hygiene; however, intervention measures are necessary to prevent parasitic infection in their domestic animals.

Abstract Background The net benefit of aspirin cessation in older adults remains uncertain. This study aimed to use observational data to emulate a randomized trial of aspirin cessation versus continuation in older adults without cardiovascular disease (CVD). Methods Post hoc analysis using a target trial emulation framework applied to the immediate post-trial period (2017–2021) of a study of low-dose aspirin initiation in adults aged ≥ 70 years (ASPREE; NCT01038583). Participants from Australia and the USA were included if they were free of CVD at the start of the post-trial intervention period (time zero, T0) and had been taking open-label or randomized aspirin immediately before T0. The two groups in the target trial were as follows: aspirin cessation (participants who were taking randomized aspirin immediately before T0; assumed to have stopped at T0 as instructed) versus aspirin continuation (participants on open-label aspirin at T0 regardless of their randomized treatment; assumed to have continued at T0). The outcomes after T0 were incident CVD, major adverse cardiovascular events (MACE), all-cause mortality, and major bleeding during 3, 6, and 12 months (short-term) and 48 months (long-term) follow-up. Hazard ratios (HRs) comparing aspirin cessation to continuation were estimated from propensity-score (PS) adjusted Cox proportional-hazards regression models. Results We included 6103 CVD-free participants (cessation: 5427, continuation: 676). Over both short- and long-term follow-up, aspirin cessation versus continuation was not associated with elevated risk of CVD, MACE, and all-cause mortality (HRs, at 3 and 48 months respectively, were 1.23 and 0.73 for CVD, 1.11 and 0.84 for MACE, and 0.23 and 0.79 for all-cause mortality, p > 0.05), but cessation had a reduced risk of incident major bleeding events (HRs at 3 and 48 months, 0.16 and 0.63, p

With a fast growing group of medications in the therapeutic field, monoclonal antibodies (MAbs) have a wide range of current and potential therapeutic applications. Several efforts have been made to the development of human MAbs since antibodies of human origin are expected to be superior to others. This review focuses on the usage of MAbs in the treatment of oral cancer and their limitations. The literature search was done in various search engines such as Medline, PubMed, Wiley, Science Direct, and Scopus. The keywords used for the database search were immunotherapy, MAbs, and oral cancer therapy. A total of 80 articles appeared from various sources, of which 60 were subjected to screening and 36 were related to the research objective. Based on the literature search, it is evident that MAbs can be used for oral cancer management and is under clinical trials for future prospects.

Abstract Multistate memory systems have the ability to store and process more data in the same physical space as binary memory systems, making them a potential alternative to existing binary memory systems. In the past, it has been demonstrated that voltage-controlled magnetic anisotropy (VCMA) based writing is highly energy-efficient compared to other writing methods used in non-volatile nano-magnetic binary memory systems. In this study, we introduce a new, VCMA-based and skyrmion-mediated non-volatile ternary memory system using a perpendicular magnetic tunnel junction (p-MTJ) in the presence of room temperature thermal perturbation. We have also shown that ternary states {− 1, 0, + 1} can be implemented with three magnetoresistance values obtained from a p-MTJ corresponding to ferromagnetic up, down, and skyrmion state, with 99% switching probability in the presence of room temperature thermal noise in an energy-efficient way, requiring ~ 2 fJ energy on an average for each switching operation. Additionally, we show that our proposed ternary memory demonstrates an improvement in area and energy by at least 2X and ~ 104X respectively, compared to state-of-the-art spin-transfer torque (STT)-based non-volatile magnetic multistate memories. Furthermore, these three states can be potentially utilized for energy-efficient, high-density in-memory quantized deep neural network implementation.

Abstract Lymphatic filariasis (LF) is a crippling and disfiguring parasitic condition. India accounts for 55% of the world’s LF burden. The filarial parasite Wuchereria bancrofti is known to cause 99.4% of the cases while, Brugia malayi accounts for 0.6% of the issue occurring mainly in some pockets of Odisha and Kerala states. The Balasore (Baleswar) district of Odisha has been a known focus of B. malayi transmission. We employed molecular xenomonitoring to detect filarial parasite DNA in vectors. In six selected villages, Gravid traps were used to collect Culex mosquitoes and hand catch method using aspirators was followed for collection of mansonioides. A total of 2903 mosquitoes comprising of Cx. quinquefasciatus (n = 2611; 89.94%), Cx. tritaeniorhynchus (n = 100; 3.44%), Mansonia annuliferea (n = 139; 4.78%) and Mansonia uniformis (n = 53; 1.82%) were collected from six endemic villages. The species wise mosquitoes were made into 118 pools, each with a maximum of 25 mosquitoes, dried and transported to the laboratory at VCRC, Puducherry. The mosquito pools were subjected to parasite DNA extraction, followed by Real-time PCR using LDR and HhaI probes to detect W. bancrofti and B. malayi infections, respectively. Seven pools (6.66%) of Cx. quinquefasciatus, showed infection with only W. bancrofti while none of the pools of other mosquito species showed infection with either W. bancrofti or B. malayi. Although the study area is endemic to B. malayi, none of the vectors of B. malayi was found with parasite infection. This study highlights the ongoing transmission of bancroftian filariasis in the study villages of Balasore district of Odisha and its implications for evaluating LF elimination programme.

Introduction: Visual perception plays a pivotal role in a child’s overall development and learning. Occupational therapists often employ interventions to support children in enhancing their visual perception skills, with technology-based approaches gaining prominence in recent years. This review intends to highlight the significance of visual perception interventions, especially those involving technology. Aim: To systematically synthesise the literature on the effectiveness of technology-based interventions on visual perception in children with disabilities aged 4-12 years. Materials and Methods: A comprehensive search of studies was conducted using electronic databases (Scopus, PubMed, ProQuest, and OTseeker). Additionally, studies were also considered through manual searches from printed journals (American Journal of Occupational Therapy, British Journal of Occupational Therapy, Canadian Journal of Occupational Therapy, and the Australian Journal of Occupational Therapy) to identify existing technology-based visual perception interventions in children aged 4-12 years. Risk of Bias was conducted through guidelines for systematic review by the American Occupational Therapy Association (AOTA). Data extraction was reported by tabulating author(s) and year, sample characteristics, outcome measures used, study design, intervention details (experimental, comparator, study setting, duration), and outcomes of the studies. Results: In the present review of 13 studies, two studies used iPad interventions, while 11 used computer-based interventions, targeting various clinical groups like developmental delays, dyslexia, cerebral palsy, hearing impairment, down syndrome, hydrocephalus, and special needs. Occupational therapists led most studies, with some involving physiotherapists, educators, and multidisciplinary teams. iPad interventions focused on visual skills with structured apps, while computer methods included games and software like Microsoft Office and Computerised Visual Perception Training (CVPT) for visual training. Positive effects were seen on visual perception and motor skills across different conditions with these technology-based interventions. Conclusion: Visual perception interventions, particularly those incorporating technology, have become invaluable in the field of paediatric occupational therapy. As technology continues to evolve, occupational therapists must remain adaptive and innovative in their strategies to provide the best possible support for children with visual perception difficulties.

Abstract Rice blast disease is the most devastating disease constraining crop productivity. Vertical resistance to blast disease is widely studied despite its instability. Clusters of genes or QTLs conferring blast resistance that offer durable horizontal resistance are important in resistance breeding. In this study, we aimed to refine the reported QTLs and identify stable meta-QTLs (MQTLs) associated with rice blast resistance. A total of 435 QTLs were used to project 71 MQTLs across all the rice chromosomes. As many as 199 putative rice blast resistance genes were identified within 53 MQTL regions. The genes included 48 characterized resistance gene analogs and related proteins, such as NBS–LRR type, LRR receptor-like kinase, NB-ARC domain, pathogenesis-related TF/ERF domain, elicitor-induced defense and proteins involved in defense signaling. MQTL regions with clusters of RGA were also identified. Fifteen highly significant MQTLs included 29 candidate genes and genes characterized for blast resistance, such as Piz, Nbs-Pi9, pi55-1, pi55-2, Pi3/Pi5-1, Pi3/Pi5-2, Pikh, Pi54, Pik/Pikm/Pikp, Pb1 and Pb2. Furthermore, the candidate genes (42) were associated with differential expression (in silico) in compatible and incompatible reactions upon disease infection. Moreover, nearly half of the genes within the MQTL regions were orthologous to those in O. sativa indica, Z. mays and A. thaliana, which confirmed their significance. The peak markers within three significant MQTLs differentiated blast-resistant and susceptible lines and serve as potential surrogates for the selection of blast-resistant lines. These MQTLs are potential candidates for durable and broad-spectrum rice blast resistance and could be utilized in blast resistance breeding.

Abstract High-altitude (HA) environment presents immense physiological adversities for humans that have been overcome by supplementing bio-active phytochemicals from functional foods that support and accelerate acclimatization under these extreme environmental conditions. Several agricultural interventions have been investigated to enhance the phytochemical content in vegetables however; these studies have been limited to low-altitude (LA) regions only. In view of an existing knowledge gap, current work is designed to compare the phytochemical compositions of HA and LA-grown Brassicaceae vegetables (cabbage, cauliflower, knol-khol, and radish) using organic treatments via farm yard manure (FYM) and Azotobacter. The open field study was conducted as a two-factorial randomized block design. The first factor was treatment (T1-FYM, T2-Azotobacter, T3-FYM + Azotobacter, and T4-control) while the second was locations (HA and LA). Among all these treatments, the application of treatment T3 in HA-grown cabbage showed the highest total phenolic content (TPC; 9.56 μg/mg), total flavonoids content (TFC; 14.48 μg/mg), and antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH; 85.97%) and ferric reducing antioxidant power (FRAP; 30.77 μg/mg) compared to LA grown samples. Reverse Phase high performance liquid chromatography (RP-HPLC) analysis showed that treatment T3 at HA led to significantly high kaempferol (0.92 μg/mg) and sulforaphane (8.94 μg/mg) contents in cabbage whereas, indole-3-carbinol (1.31 μg/mg) was higher in HA grown cauliflower. The present study provides scientific evidence for the enrichment of health-promoting phytochemical compounds in Brassicaceae vegetables grown with T3 treatment specifically at HA.

Interstitial Lung Disease (ILD) in diffuse cutaneous Systemic Sclerosis (SSc) patients is present in about 53% of cases and 35% in cases with limited cutaneous SSc. Even though there are only a few case reports of ILD associated with Hereditary Haemorrhagic Telangiectasia (HHT), a direct association of SSc and HHT has not been reported. Hence, little is known about the pathogenetic link between the two diseases. In this case report, a 24 years old married female patient presented with progressive breathlessness for one year. Initial evaluation of the patient suggested the diagnosis of SSc based on clinical findings like sclerodactyly along with telangiectasia. High Resolution Computed Tomography (HRCT) of the chest revealed the presence of pulmonary arteriovenous malformation along with ILD. Past medical history and family history of epistaxis for three generations suggested the diagnosis of HHT. This case reports a rare association of Connective Tissue Disease (CTD) and an autosomal disorder of vascular dysplasia with overlapping features of telangiectasia. This case is presented to highlight the possible association of HHT with CTDs. With the currently available literature, an attempt is made to find a plausible pathogenetic link between HHT and SSc. Transforming Growth Factor-β (TGF-β) is a pleiotropic growth factor that regulates the growth and differentiation of various cell types, and immune regulation has been implicated in the pathogenesis of both HHT and SSc. The other pathogenetic mechanism common to both diseases is impaired nitric oxide-mediated vasodilation. This case report emphasises the need for further research for a better understanding of the pathogenesis of both diseases.

Background: The symptoms of hemorrhoids has a wide spectrum that ranges from bleeding per rectum, itching, prolapse of the hemorrhoidal cushions, thrombosis leading to pain and discomfort, incontinence especially of fluids and mucous discharge. But for surgical management, hemorrhoidectomy is more sustainable in symptom control and has been considered as gold standard. Rubber band ligation has been the most popular amongst non-surgical interventions because of its effectiveness, minimal complication rate and shorter recover period compared to operative procedures. The present study aimed at determining the efficacy and safety of rubber banding ligation procedure versus the standard hemorrhoidectomy for second degree hemorrhoids. Methods: A prospective randomised comparative study was conducted at Department of Surgery, Indira Gandhi Medical College Hospital, Puducherry for 18 months after getting institutional ethical committee clearance. A total no. of 74 cases of 2nd degree hemorrhoids were divided into two groups A (open hemorrhoidectomy) and B (banding) by simple randomization technique of 37 each. A detailed history & examination including per rectal and Proctoscopy examination was done of each patient and the data entered in the pro forma. The description of data was expressed in the form of mean±SD for quantitative data, while frequency and proportion for qualitative data. Results: Totally male patients represented 57% compared to female with 43% 78% of the patients who underwent banding did not have any immediate post-operative complications while only 4 persons reported pain and bleeding post-operatively compared to 12 patients reporting bleeding and 11 patients reporting pain after hemorrhoidectomy. The duration of hospital stay post procedures was found to be lesser in banding group compared to hemorrhoidectomy group with p value being statistically highly significant (p

Background: Peripheral nerve blockade is a crucial component of comprehensive anesthetic care, providing effective analgesia with fewer side effects than opioids and other oral analgesics. Aims and Objectives: This study investigated the effectiveness of a magnesium adjuvant in an ultrasound-guided supraclavicular brachial plexus block to determine the minimum dose required for the desired effects. Materials and Methods: This prospective, double-blind, and randomized controlled study included 90 patients scheduled for elective upper limb orthosurgery at GTMCH. The 90 patients were divided into three groups: A, B, and C. Each group received 21.5 mL of the test drug, with A receiving ropivacaine and normal saline, B receiving ropivacaine and magnesium, and C receiving ropivacaine and magnesium. Results: There was a significant difference in Group A between the onset of sensory and motor blockade in Groups B and C. There was a significant difference in Group A between the duration of sensory and motor blockade in Groups B and C, but no significant difference was observed between Groups B and C. The visual analog scale score varied from 0 to 6 in Group A and from 0 to 5 in Groups B and C. It was found to be significant only from the 7th to 10th h. There was a significant difference in Group A between Groups B and C in 1st rescue analgesia post-operatively and the number of rescue injections in the first 24 h, but no significant differences was observed between Groups B and C. Conclusion: Both 100 and 150 mg magnesium sulfate with 0.5% ropivacaine in supraclavicular blocks prolonged the blockade duration and reduced analgesic needs.

Background: Clinical laboratories function to deliver accurate, reliable, and timely reported results which are used in decision-making, diagnosis, and monitoring. Sigma metrics help to assess analytic methodologies and provide benchmarks for laboratories to design protocols for internal quality control (IQC), address poor assay performance, and assess the efficiency of existing laboratory processes. Thus, this study was undertaken to estimate the coefficient of variation (CV%), bias%, and total allowable error (TEa) of quality control (QC) samples using EM 200, to compare the TEa using Clinical Laboratories Improvement Act (CLIA) guidelines, and to analyze the sigma metrics of level 1 QC samples. Aims and Objectives: The aims and objectives of the study are to estimate the CV%, Bias%, TEa of QC samples using EM 200, to compare the TEa using CLIA guidelines, and to analyze the sigma metrics of level 1 QC samples. Materials and Methods: A cross-sectional study was carried out in the Central Biochemistry Lab, Karwar Institute of Medical Sciences, Karwar. IQC data level 1 of 15 analytes was analyzed using EM200 for 3 months. CV% is calculated from internal quality data, whereas bias% is obtained from an external quality assurance program. Sigma metrics were calculated using bias% and CV%. TEa was calculated and compared with CLIA guidelines. Results: We have < 3 sigma values (unstable, unacceptable) for urea, creatinine, BID, serum glutamic oxaloacetic transaminase, serum glutamate pyruvate transaminase (SGPT), protein, cholesterol, calcium, 3–6 (ideal) for glucose, uric acid, total bilirubin, alkaline phosphatase, albumin, high-density lipoprotein, and >6 (excellent) for triglycerides. TEa observed less than or close to CLIA suggests quality requirement met, and TEa observed more than CLIA (urea, creatinine, BID, SGPT, protein, and calcium) suggests methodologies need evaluation. Conclusion: Sigma metrics help to assess analytical methodologies and augment laboratory performance. Each and every laboratory can use sigma metrics as a guideline for QC strategy and a self-assessment tool for proper functioning.