1. Expression and biological characteristics of long non-coding RNA AL365181.2 in cholangiocarcinoma
- Author
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WANG Qinlei, SUN Zhaowei, GUO Jingyun, LI Haoran, FENG Yujie, ZHANG Bingyuan
- Subjects
bile duct neoplasms ,rna, long noncoding ,gene expression ,cell proliferation ,cell movement ,neoplasm invasiveness ,carcinogenesis ,Medicine - Abstract
Objective To investigate the expression level of long non-coding RNA (lncRNA) AL365181.2 in cholangiocarcinoma (CHOL) and its effect on the functional characteristics of CHOL cells. Methods The Cancer Genome Atlas (TCGA) database was used to compare the expression level of lncRNA AL365181.2 between cancerous tissue and paracancerous tissue in CHOL patients, and the association between the expression level of lncRNA AL365181.2 and immune cell infiltration in CHOL tissue was analyzed. The Gene Set Enrichment Analysis (GSEA) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed for lncRNA AL365181.2. EdU assay, wound healing assay, Transwell assay, and xenograft tumor formation experiment were used to investigate the impact of lncRNA AL365181.2 on the proliferation, migration, invasion, and tumorigenic abilities of CHOL cells. Results The analysis of TCGA data showed that the expression level of lncRNA AL365181.2 in CHOL tissue was significantly higher than that in paracancerous tissue (Z=4.00,P
- Published
- 2024
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