37 results on '"P. Weeks"'
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2. Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing
- Author
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James L. Van Etten, Donald P. Weeks, and Eric A. Noel
- Subjects
0106 biological sciences ,food.ingredient ,Science ,Mutant ,Agrobacterium ,Chlorella ,Biology ,01 natural sciences ,Genome ,03 medical and health sciences ,Viral Proteins ,food ,Transformation, Genetic ,Genome editing ,Chlorovirus ,010608 biotechnology ,CRISPR-Associated Protein 9 ,CRISPR ,Phycodnaviridae ,Gene ,030304 developmental biology ,Genetics ,Gene Editing ,0303 health sciences ,Multidisciplinary ,030306 microbiology ,Cas9 ,DNA Viruses ,Transfection ,Electroporation ,Ribonucleoproteins ,Medicine ,CRISPR-Cas Systems ,Research Article - Abstract
Genetic and molecular modifications of the large dsDNA chloroviruses, with genomes of 290 to 370 kb, would expedite studies to elucidate the functions of both identified and unidentified virus-encoded proteins. These plaque-forming viruses replicate in certain unicellular, eukaryotic chlorella-like green algae. However, to date, only a few of these algal species and virtually none of their viruses have been genetically manipulated due to lack of practical methods for genetic transformation and genome editing. Attempts at using Agrobacterium-mediated transfection of chlorovirus host Chlorella variabilis NC64A with a specially-designed binary vector resulted in successful transgenic cell selection based on expression of a hygromycin-resistance gene, initial expression of a green fluorescence gene and demonstration of integration of Agrobacterium T-DNA. However, expression of the integrated genes was soon lost. To develop gene editing tools for modifying specific chlorovirus CA-4B genes using preassembled Cas9 protein-sgRNA ribonucleoproteins (RNPs), we tested multiple methods for delivery of Cas9/sgRNA RNP complexes into infected cells including cell wall-degrading enzymes, electroporation, silicon carbide (SiC) whiskers, and cell-penetrating peptides (CPPs). In one experiment two independent virus mutants were isolated from macerozyme-treated NC64A cells incubated with Cas9/sgRNA RNPs targeting virus CA-4B-encoded gene 034r, which encodes a glycosyltransferase. Analysis of DNA sequences from the two mutant viruses showed highly targeted nucleotide sequence modifications in the 034r gene of each virus that were fully consistent with Cas9/RNP-directed gene editing. However, in ten subsequent experiments, we were unable to duplicate these results and therefore unable to achieve a reliable system to genetically edit chloroviruses. Nonetheless, these observations provide strong initial suggestions that Cas9/RNPs may function to promote editing of the chlorovirus genome, and that further experimentation is warranted and worthwhile.
- Published
- 2021
3. Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry
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Jitendra PS. Sawhney, Veerappa A. Kothiwale, Vikas Bisne, Rajashekhar Durgaprasad, Praveen Jadhav, Manoj Chopda, Velam Vanajakshamma, Ramdhan Meena, Govindan Vijayaraghavan, Kamaldeep Chawla, Jagan Allu, Karen S. Pieper, A. John Camm, Ajay K. Kakkar, Jean-Pierre Bassand, David A. Fitzmaurice, Samuel Z. Goldhaber, Shinya Goto, Sylvia Haas, Werner Hacke, Lorenzo G. Mantovani, Frank Misselwitz, Alexander G.G. Turpie, Martin van Eickels, Freek W.A. Verheugt, Gloria Kayani, Keith A.A. Fox, Bernard J. Gersh, Hector Lucas Luciardi, Harry Gibbs, Marianne Brodmann, Frank Cools, Antonio Carlos Pereira Barretto, Stuart J. Connolly, Alex Spyropoulos, John Eikelboom, Ramon Corbalan, Dayi Hu, Petr Jansky, Jørn Dalsgaard Nielsen, Hany Ragy, Pekka Raatikainen, Jean-Yves Le Heuzey, Harald Darius, Matyas Keltai, Sanjay Kakkar, Jitendra Pal Singh Sawhney, Giancarlo Agnelli, Giuseppe Ambrosio, Yukihiro Koretsune, Carlos Jerjes Sánchez Díaz, Hugo Ten Cate, Dan Atar, Janina Stepinska, Elizaveta Panchenko, Toon Wei Lim, Barry Jacobson, Seil Oh, Xavier Viñolas, Marten Rosenqvist, Jan Steffel, Pantep Angchaisuksiri, Ali Oto, Alex Parkhomenko, Wael Al Mahmeed, David Fitzmaurice, D.Y. Hu, K.N. Chen, Y.S. Zhao, H.Q. Zhang, J.Z. Chen, S.P. Cao, D.W. Wang, Y.J. Yang, W.H. Li, Y.H. Yin, G.Z. Tao, P. Yang, Y.M. Chen, S.H. He, Ying Wang, Yong Wang, G.S. Fu, X. Li, T.G. Wu, X.S. Cheng, X.W. Yan, R.P. Zhao, M.S. Chen, L.G. Xiong, P. Chen, Y. Jiao, Y. Guo, L. Xue, F.Z. Wang, H. Li, Z.M. Yang, C.L. Bai, J. Chen, J.Y. Chen, X. Chen, S. Feng, Q.H. Fu, X.J. Gao, W.N. Guo, R.H. He, X.A. He, X.S. Hu, X.F. Huang, B. Li, J. Li, L. Li, Y.H. Li, T.T. Liu, W.L. Liu, Y.Y. Liu, Z.C. Lu, X.L. Luo, T.Y. Ma, J.Q. Peng, X. Sheng, X.J. Shi, Y.H. Sun, G. Tian, K. Wang, L. Wang, R.N. Wu, Q. Xie, R.Y. Xu, J.S. Yang, L.L. Yang, Q. Yang, Y. Ye, H.Y. Yu, J.H. Yu, T. Yu, H. Zhai, Q. Zhan, G.S. Zhang, Q. Zhang, R. Zhang, Y. Zhang, W.Y. Zheng, B. Zhou, Z.H. Zhou, X.Y. Zhu, S. Kakkar, J.P.S. Sawhney, P. Jadhav, R. Durgaprasad, A.G. Ravi Shankar, R.K. Rajput, K. Bhargava, R. Sarma, A. Srinivas, D. Roy, U.M. Nagamalesh, M. Chopda, R. Kishore, G. Kulkarni, P. Chandwani, R.A. Pothiwala, M. Padinhare Purayil, S. Shah, K. Chawla, V.A. Kothiwale, B. Raghuraman, G. Vijayaraghavan, V.M. Vijan, G. Bantwal, V. Bisne, A. Khan, J.B. Gupta, S. Kumar, D. Jain, S. Abraham, D. Adak, A. Barai, H. Begum, P. Bhattacharjee, M. Dargude, D. Davies, B. Deshpande, P. Dhakrao, V. Dhyani, S. Duhan, M. Earath, A. Ganatra, S. Giradkar, V. Jain, R. Karthikeyan, L. Kasala, S. Kaur, S. Krishnappa, A. Lawande, B. Lokesh, N. Madarkar, R. Meena, P. More, D. Naik, K. Prashanth, M. Rao, N.M. Rao, N. Sadhu, D. Shah, M. Sharma, P. Shiva, S. Singhal, S. Suresh, V. Vanajakshamma, S.G. Panse, Y. Koretsune, S. Kanamori, K. Yamamoto, K. Kumagai, Y. Katsuda, K. Sadamatsu, F. Toyota, Y. Mizuno, I. Misumi, H. Noguchi, S. Ando, T. Suetsugu, M. Minamoto, Hiroshi Oda, K. Shiraishi, S. Adachi, K. Chiba, H. Norita, M. Tsuruta, T. Koyanagi, H. Ando, T. Higashi, K. Okada, S. Azakami, S. Komaki, K. Kumeda, T. Murayama, J. Matsumura, Y. Oba, R. Sonoda, K. Goto, K. Minoda, Y. Haraguchi, H. Suefuji, H. Miyagi, H. Kato, Tadashi Nakamura, Tsugihiro Nakamura, H. Nandate, R. Zaitsu, Yoshihisa Fujiura, A. Yoshimura, H. Numata, J. Ogawa, H. Tatematsu, Y. Kamogawa, K. Murakami, Y. Wakasa, M. Yamasawa, H. Maekawa, S. Abe, H. Kihara, S. Tsunoda, Katsumi Saito, Kazuyuki Saito, T. Fudo, K. Obunai, H. Tachibana, I. Oba, T. Kuwahata, S. Higa, M. Gushiken, T. Eto, H. Yoshida, D. Ikeda, Yoshitake Fujiura, M. Ishizawa, M. Nakatsuka, K. Murata, C. Ogurusu, M. Shimoyama, M. Akutsu, I. Takamura, F. Hoshino, N. Yokota, T. Iwao, K. Tsuchida, M. Takeuchi, Y. Hatori, Y. Kitami, Yoichi Nakamura, R. Oyama, M. Ageta, Hiroyuki Oda, Y. Go, K. Mishima, T. Unoki, S. Morii, Yuhei Shiga, H. Sumi, T. Nagatomo, K. Sanno, K. Fujisawa, Y. Atsuchi, T. Nagoshi, T. Seto, T. Tabuchi, M. Kameko, K. Nii, K. Oshiro, H. Takezawa, S. Nagano, N. Miyamoto, M. Iwaki, Yuichiro Nakamura, M. Fujii, M. Okawa, Masahiko Abe, Masatake Abe, Mitsunori Abe, T. Saito, T. Mito, K. Nagao, J. Minami, T. Mita, I. Sakuma, T. Taguchi, S. Marusaki, H. Doi, M. Tanaka, T. Fujito, M. Matsuta, T. Kusumoto, S. Kakinoki, K. Ashida, N. Yoshizawa, J. Agata, O. Arasaki, M. Manita, M. Ikemura, S. Fukuoka, H. Murakami, S. Matsukawa, Y. Hata, T. Taniguchi, T. Ko, H. Kubo, M. Imamaki, M. Akiyama, M. Inagaki, H. Odakura, T. Ueda, Y. Katsube, A. Nakata, H. Watanabe, M. Techigawara, M. Igarashi, K. Taga, T. Kimura, S. Tomimoto, M. Shibuya, M. Nakano, K. Ito, T. Seo, S. Hiramitsu, H. Hosokawa, M. Hoshiai, M. Hibino, K. Miyagawa, Hajime Horie, N. Sugishita, Yukio Shiga, A. Soma, K. Neya, Tetsuro Yoshida, Tomoki Yoshida, M. Mizuguchi, M. Ishiguro, T. Minagawa, M. Wada, H. Mukawa, F. Okuda, S. Nagasaka, Y. Abe, Sen Adachi, Susumu Adachi, T. Adachi, K. Akahane, T. Amano, K. Aoki, T. Aoyama, H. Arai, S. Arima, T. Arino, H. Asano, T. Asano, J. Azuma, T. Baba, T. Betsuyaku, H. Chibana, H. Date, J. Doiuchi, Y. Emura, M. Endo, Y. Fujii, R. Fujiki, A. Fujisawa, Y. Fujisawa, T. Fukuda, T. Fukui, N. Furukawa, T. Furukawa, W. Furumoto, T. Goto, M. Hamaoka, N. Hanazono, K. Hasegawa, T. Hatsuno, Y. Hayashi, K. Higuchi, K. Hirasawa, H. Hirayama, M. Hirose, S. Hirota, M. Honda, Hideki Horie, T. Ido, O. Iiji, H. Ikeda, K. Ikeda, K. Ikeoka, M. Imaizumi, H. Inaba, T. Inoue, F. Iseki, A. Ishihara, N. Ishioka, N. Ito, T. Iwase, H. Kakuda, J. Kamata, H. Kanai, H. Kanda, M. Kaneko, H. Kano, T. Kasai, T. Kato, Y. Kato, Y. Kawada, K. Kawai, K. Kawakami, S. Kawakami, T. Kawamoto, S. Kawano, J. Kim, T. Kira, H. Kitazawa, H. Kitazumi, T. Kito, T. Kobayashi, T. Koeda, J. Kojima, H. Komatsu, I. Komatsu, Y. Koshibu, T. Kotani, T. Kozuka, Y. Kumai, T. Kumazaki, I. Maeda, K. Maeda, Y. Maruyama, S. Matsui, K. Matsushita, Y. Matsuura, K. Mineoi, H. Mitsuhashi, N. Miura, S. Miyaguchi, S. Miyajima, H. Miyamoto, A. Miyashita, S. Miyata, I. Mizuguchi, A. Mizuno, T. Mori, O. Moriai, K. Morishita, O. Murai, Sho Nagai, Shunichi Nagai, E. Nagata, H. Nagata, A. Nakagomi, S. Nakahara, M. Nakamura, R. Nakamura, N. Nakanishi, T. Nakayama, R. Nakazato, T. Nanke, J. Nariyama, Y. Niijima, H. Niinuma, Y. Nishida, Y. Nishihata, K. Nishino, H. Nishioka, K. Nishizawa, I. Niwa, K. Nomura, S. Nomura, M. Nozoe, T. Ogawa, N. Ohara, M. Okada, K. Okamoto, H. Okita, M. Okuyama, H. Ono, T. Ono, Y. Onuki Pearce, S. Oriso, A. Ota, E. Otaki, Y. Saito, H. Sakai, N. Sakamoto, Y. Sakamoto, Y. Samejima, Y. Sasagawa, H. Sasaguri, A. Sasaki, T. Sasaki, Kazuki Sato, Kiyoharu Sato, M. Sawano, S. Seki, Y. Sekine, Y. Seta, K. Sezaki, N. Shibata, Y. Shiina, H. Shimono, Y. Shimoyama, T. Shindo, H. Shinohara, R. Shinohe, T. Shinozuka, T. Shirai, T. Shiraiwa, Y. Shozawa, T. Suga, C. Sugimoto, Kazuo Suzuki, Keita Suzuki, Shu Suzuki, Shunji Suzuki, Susumu Suzuki, Y. Suzuki, M. Tada, A. Taguchi, T. Takagi, Y. Takagi, K. Takahashi, S. Takahashi, H. Takai, C. Takanaka, S. Take, H. Takeda, K. Takei, K. Takenaka, T. Tana, G. Tanabe, K. Taya, H. Teragawa, S. Tohyo, S. Toru, Y. Tsuchiya, T. Tsuji, K. Tsuzaki, H. Uchiyama, O. Ueda, Y. Ueyama, N. Wakaki, T. Wakiyama, T. Washizuka, M. Watanabe, T. Yamada, T. Yamagishi, H. Yamaguchi, Kenichi Yamamoto, Kentaro Yamamoto, Kunihiko Yamamoto, T. Yamamoto, M. Yamaura, M. Yamazoe, K. Yasui, Y. Yokoyama, K. Yoshida, T.W. Lim, C.K. Ching, C.G. Foo, J.H. Chow, D.D. Chen, F.R. Jaufeerally, Y.M. Lee, G. Lim, W.T. Lim, S. Thng, S.Y. Yap, C. Yeo, S. Oh, H.N. Pak, J.-B. Kim, J.H. Kim, S.-W. Jang, D.H. Kim, D.R. Ryu, S.W. Park, D.-K. Kim, D.J. Choi, Y.S. Oh, M.-C. Cho, S.-H. Kim, H.-K. Jeon, D.-G. Shin, J.S. Park, H.K. Park, S.-J. Han, J.H. Sung, J.-G. Cho, G.-B. Nam, Y.K. On, H.E. Lim, J.J. Kwak, T.-J. Cha, T.J. Hong, S.H. Park, J.H. Yoon, N.-H. Kim, K.-S. Kim, B.C. Jung, G.-S. Hwang, C.-J. Kim, D.B. Kim, J.J. Ahn, H.J. An, H. Bae, A.L. Baek, W.J. Chi, E.A. Choi, E.H. Choi, H.K. Choi, H.S. Choi, S. Han, E.S. Heo, K.O. Her, S.W. Hwang, E.M. Jang, H.-S. Jang, S. Jang, H.-G. Jeon, S.R. Jeon, Y.R. Jeon, H.K. Jeong, I.-A. Jung, Hyeon Jeong Kim, Hyun Ju Kim, Ji Seon Kim, Jung Sook Kim, J.A. Kim, K.T. Kim, M.S. Kim, Sang Hee Kim, Sang Hyun Kim, Y.-I. Kim, C.S. Lee, E.H. Lee, G.H. Lee, H.Y. Lee, H.-Y. Lee, K.H. Lee, K.R. Lee, M.S. Lee, M.-Y. Lee, R.W. Lee, S.E. Lee, S.H. Lee, S. Lee, W.Y. Lee, I.K. Noh, A.R. Park, B.R. Park, H.N. Park, J.H. Park, M. Park, Y. Park, S.-Y. Seo, J. Shim, J.H. Sim, Y.M. Sohn, W.S. Son, Y.S. Son, H.J. Song, H.K. Wi, J.J. Woo, S. Ye, K.H. Yim, K.M. Yoo, E.J. Yoon, S.Y. Yun, P. Angchaisuksiri, S. Chawanadelert, P. Mongkolwongroj, K. Kanokphatcharakun, S. Cheewatanakornkul, T. Laksomya, S. Pattanaprichakul, T. Chantrarat, S. Rungaramsin, S. Silaruks, W. Wongcharoen, K. Siriwattana, K. Likittanasombat, P. Katekangplu, W. Boonyapisit, D. Cholsaringkarl, B. Chatlaong, P. Chattranukulchai, Y. Santanakorn, P. Hutayanon, P. Khunrong, T. Bunyapipat, S. Jai-Aue, P. Kaewsuwanna, P. Bamungpong, S. Gunaparn, S. Hongsuppinyo, R. Inphontan, R. Khattaroek, K. Khunkong, U. Kitmapawanont, C. Kongsin, B. Naratreekoon, S. Ninwaranon, J. Phangyota, A. Phrommintikul, P. Phunpinyosak, K. Pongmorakot, S. Poomiphol, N. Pornnimitthum, S. Pumprueg, S. Ratchasikaew, K. Sanit, K. Sawanyawisuth, B. Silaruks, R. Sirichai, A. Sriwichian, W. Suebjaksing, P. Sukklad, T. Suttana, A. Tangsirira, O. Thangpet, W. Tiyanon, Y. Vorasettakarnkij, T. Wisaratapong, W. Wongtheptien, A. Wutthimanop, S. Yawila, A. Oto, A. Altun, I. Ozdogru, K. Ozdemir, O. Yilmaz, A. Aydinlar, M.B. Yilmaz, E. Yeter, Z. Ongen, M. Cayli, H. Pekdemir, M. Ozdemir, M. Sucu, T. Sayin, M. Demir, H. Yorgun, M. Ersanli, E. Okuyan, D. Aras, H. Abdelrahman, O. Aktas, D. Alpay, F. Aras, M.F. Bireciklioglu, S. Budeyri, M. Buyukpapuc, S. Caliskan, M. Esen, M.A. Felekoglu, D. Genc, B. Ikitimur, E.B. Karaayvaz, S. Kılıç Karataş, S. Okutucu, E. Ozcelik, A. Quisi, H. Sag, L. Sahiner, B.Y. Sayin, T. Seker, D. Uzun Alkan, E. Yildirim, R. Yildirim, F. Yilmaz, V. Yuksekdag, H.L. Luciardi, N. Vensentini, A.C. Ingaramo, G.A. Sambadaro, V. Fernandez Caputi, S.G. Berman, P. Dragotto, A.J. Kleiban, N. Centurion, G. Giacomi, R.A. Ahuad Guerrero, D. Conde, G. Zapata, L.A. Di Paola, J.L. Ramos, R.D. Dran, J. Egido, A.A. Fernandez, M.J. Fosco, S. Sassone, V.A. Sinisi, L.R. Cartasegna, M.A. Berli, O.A. Gomez Vilamajo, F. Ferroni, E.D. Alaguibe, A. Alvarez D'Amelio, C. Arabetti, L. Arias, J.A. Belardi, L. Bergesio, F. Berli, M. Berli, S. Borchowiec, C. Buzzetti, R. Cabrini, V. Campisi, A.L. Cappi, R. Carrizo, F. Colombo Berra, J.P. Costabel, O.J.A. Costamagna, A.A. Damonte, I.N. De Urquiza, F. Diez, M.F. Edén, M. Fanuele, F. Fernandez Voena, M. Foa Torres, C. Funosas, M.P. Giacomi, C.H. Gimenez, E.P. Gurfinkel, M. de L.M. Had, V. Hansen, A.D. Hrabar, M. Ingratta, A. Lopez, G. Maehara, L. Maffei, A. Martinelli, C. Martinelli, J. Matkovich, B. Mautner, A. Meirino, R. Munguia, A. Navarro, V. Novas, G. Perez Prados, J. Pontoriero, R.N. Potito, C. Ricotti, M.A. Rodriguez, F. Rolandi, M.E. Said Palladino, M. Salinger, L.S. Sanziani, P.O. Schygiel, A. Sossich, J.F. Tinto, L. Tonelli, A.L. Tufare, M. Vallejo, M.E. Yunis, M. Zillo, F.J. Zurbrigk, A.C.P. Barretto, D.C. Sobral Filho, J. Jaber, D. Armaganijan, J. Faria Neto, A. Steffens, W. Kunz Sebba Barroso de Souza, J.D. de Souza Neto, J.M. Ribeiro, M. Silveira Teixeira, P.R. Ferreira Rossi, L. Pires, D. Moreira, J.C. Moura Jorge, A. Menezes Lorga Filho, L.C. Bodanese, M. Westerlund Montera, C.H. Del Carlo, T. Da Rocha Rodrigues, F.A. Alves da Costa, A. Lopes, R. Lopes, G.R. Araújo, E.R. Fernandes Manenti, J.F. Kerr Saraiva, J.C. Ferreira Braga, A. Negri, L. Souto, C. Moncada, D. Bertolim Precoma, F. Roquette, G. Reis, R.A. Ramos Filho, E. Lanna Figueiredo, R. Vieira Botelho, C. Munhoz da Fontoura Tavares, C.R. Costantini Frack, J. Abdalla Saad, H.C. Finimundi, C. Pisani, D. Chemello, M. Pereira Martins, C.C. Broilo França, F. Alban, G.B. Aranha Rosito, J.B. de Moura Xavier Moraes Junior, R.T. Tumelero, L. Nigro Maia, R. Simões de Almeida, N.C. do Carmo Borges, L.G. Gomes Ferreira, P. Agliardi, J. Alves de Oliveira Gomes, V. Araujo, M. Arruda Nakazone, T. Barbosa, S. Barroso, E. Belisario Falchetto, H. Bellotti Lopes, M.A. Benez Teixeira Lemos, G. Biazus, L. Borges Queiroz, F.E. Camazzola, M. Caporale, S. Cardoso Boscato, F. Chieza, M.O. Chokr, R. Clemente Mingireanov, N. Codonho Góes, C. Correa, M. Costa, C. Costantini Ortiz, L.S. da Silva, F. da Silva Paulitsch, J.A. da Silveira, E. Daros, G.R. de Araújo, M.I. Del Monaco, C. Dias, M.A. Dias, A.P. Drummond Wainstein, P. Ely Pizzato, D.C. Esteves, P. Fabri, T. Félix Lorenzato Fonseca, E. Fernandes, C. Fonseca, C.R. Frack Costantini, R. Franchin Ferraz, F. Freire, P. Gottardo, D. Guanaes, S. Guizzardi, E. Hettwer Magedanz, F. Igansi, F. Jannuzzi, G. Junior, D. Komar, E.G. Lino, D. Lopes, O. Lourenço da Silva Júnior, E. Lustosa, A.P. Macagnan, M.C. Marinho, M. Mazzoni, G. Melo, L. Mortari, O.M.C.C. Mouco, C. Nanzer Vital, C. Ormundo, S. Oss Emmer, E. Palmegiani, R. Pavani, L. Pereira, V.L. Pereira, R. Perreira, S. Poletti, S.C. Quaia Fortunato, C. Queirantes, N. Ramos Pereira, R.L. Rech, S. Ribeiro, A. Rodrigues, H. Roesch, T. Ruaro Reichert, D. Santos, I. Santos, M. Santos, M.V. Seroqui, S. Silva, L. Soares, L. Spolaor, C. Stoll, N. Toazza Duda, L. Trama, B. Unterkircher, M.V. Valois, T. Vargas, T. Viana, C. Vicente, L. Vidal Armaganijan, R. Vieira Homem, L.G. Vieira Torres, L. Vila Boas, F. Villaça Guimarães Filho, R. Corbalan, G. Eggers, C. Bugueño Gutiérrez, G. Arriagada, S. Potthoff Cardenas, B.A.J. Stockins Fernandez, C. Conejeros, C. 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Avena, M. Chavarria, I. Espinosa, F. Flores Silva, R.H. Garcia Nava, K. Godoy, E.J. Gonzalez Felix, C.L. Gonzalez Garcia, L.G. Gonzalez Salas, P. Guajardo, S. Hernandez Gonzalez, T. Izquierdo, M.C. Mancilla Ortiz, D. Martinez Vasquez, N. Mendoza, J. Morales, N. Nikitina, S. Ochoa Aybar, A. Ortiz, P. Padilla Macias, F. Perez, J.A. Perez Sanchez, S. Piña Toledano, M. Ramos Gonzalez, C. Rivera Ramos, V. Roa Castro, G. Romero Cardona, M. Ruiz Cornejo, A. Salinas, G. Santana, P. Sida Perez, A.C. Tovar Castaneda, R. Trujillo Cortes, M. Brodmann, K. Lenz, H. Drexel, J. Foechterle, C. Hagn, A. Podczeck-Schweighofer, K. Huber, M. Winkler, B. Schneeweiß, A. Gegenhuber, W. Lang, S. Eichinger-Hasenauer, P. Kaserer, J. Sykora, H. Rasch, M. Pichler, E. Schaflinger, B. Strohmer, R. Breier, K.-M. Ebner, L. Eischer, F. Freihoff, A. Lischka-Lindner, T. Mark, A. Mirtl, A. Said, C. Stöcklöcker, B. Vogel, A. Vonbank, C. Wöhrer, D. Zanolin, F. Cools, G. Paparella, P. Vandergoten, J.-L. Parqué, L. 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Y., Angchaisuksiri, P., Chawanadelert, S., Mongkolwongroj, P., Kanokphatcharakun, K., Cheewatanakornkul, S., Laksomya, T., Pattanaprichakul, S., Chantrarat, T., Rungaramsin, S., Silaruks, S., Wongcharoen, W., Siriwattana, K., Likittanasombat, K., Katekangplu, P., Boonyapisit, W., Cholsaringkarl, D., Chatlaong, B., Chattranukulchai, P., Santanakorn, Y., Hutayanon, P., Khunrong, P., Bunyapipat, T., Jai-Aue, S., Kaewsuwanna, P., Bamungpong, P., Gunaparn, S., Hongsuppinyo, S., Inphontan, R., Khattaroek, R., Khunkong, K., Kitmapawanont, U., Kongsin, C., Naratreekoon, B., Ninwaranon, S., Phangyota, J., Phrommintikul, A., Phunpinyosak, P., Pongmorakot, K., Poomiphol, S., Pornnimitthum, N., Pumprueg, S., Ratchasikaew, S., Sanit, K., Sawanyawisuth, K., Silaruks, B., Sirichai, R., Sriwichian, A., Suebjaksing, W., Sukklad, P., Suttana, T., Tangsirira, A., Thangpet, O., Tiyanon, W., Vorasettakarnkij, Y., Wisaratapong, T., Wongtheptien, W., Wutthimanop, A., Yawila, S., Oto, A., Altun, A., Ozdogru, I., Ozdemir, K., Yilmaz, O., Aydinlar, A., Yilmaz, M. 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A., Andersson, A., Malmqvist, L., Martinsson, B., Bernsten, F., Engdahl, J., Thulin, J., Hot-Bjelac, A., Stalby, P., Aaröe, H., Ahbeck, E., Ahlmark, H., Al-Khalili, F., Bonkowski, G., Dzeletovic, S., Ekstrand, A. -B., Eriksson, G. -B., Floren, K., Grässjö, C., Hahn, S., Jaensson, P., Jansson, B., Jansson, J. -H., Kangert, R. -M., Koch, A., Kusiak, D., Lettenström, A., Lindberg, A., Lindholm, C. -J., Mannermyr, A., Mansson, K., Millborg, M., Nilsson, C., Ohlin, A. -M., Olofsson, A., Osberg, A., Pedersen, A., Risbecker, K., Rosenberg, K., Samuelsson, J., Shayesteh, M., Skoglund, K., Stjernberg, M., Thorsen, C., Steffel, J., Beer, J. H., Debrunner, J., Amstutz, D., Bruegger, J., Elise, G., Grau, A., Guinand, A., Henriette, I., Saga, E., Winnik, S., Parkhomenko, A., Rudyk, I., Tseluyko, V., Karpenko, O., Zhurba, S., Kraiz, I., Kupnovytska, I., Serediuk, N., Mostovoy, Y., Ushakov, O., Koval, O., Kovalskyi, I., Svyshchenko, Y., Sychov, O., Stanislavchuk, M., Kraydashenko, O., Yagensky, A., Tykhonova, S., Fushtey, I., Belegai, R., Berko, G., Burdeuna, L., Chabanna, O., Daniuk, I., Ivanov, A., Kamenska, E., Kaplan, P., Khyzhnyak, O., Kizim, S., Matova, O., Medentseva, O., Mochonyi, V., Mospan, M., Nemtsova, V., Ovdiienko, T., Palamarchuk, O., Pavelko, M., Petrovskyy, R., Plevak, D., Proshak, O., Pyvovar, S., Rasputina, L., Romanenko, O., Romanova, O., Sapatyi, A., Shumakov, O., Stets, R., Todoriuk, L., Varenov, V., Fitzmaurice, D., Chauhan, N., Goodwin, D., Saunders, P., Evans, R., Leese, J., Jhittay, P. S., Ross, A., Kainth, M. S., Pickavance, G., Mcdonnell, J., Williams, A., Gooding, T., Wagner, H., Suryani, S., Singal, A., Sircar, S., Bilas, R., Hutchinson, P., Wakeman, A., Stokes, M., Paul, N., Aziz, M., Ramesh, C., Wilson, P., Franklin, S., Fairhead, S., Thompson, J., St Joseph, V., Taylor, G., Tragen, D., Seamark, D., Paul, C., Richardson, M., Jefferies, A., Sharp, H., Jones, H., Giles, C., Page, M., Oginni, O., Aldegather, J., Wetherwell, S., Lumb, W., Evans, P., Scouller, F., Macey, N., Stipp, Y., West, R., Thurston, S., Wadeson, P., Matthews, J., Pandya, P., Gallagher, A., Railton, T., Sinha, B., Russell, D., Davies, J. A., Ainsworth, P., Jones, C. P., Weeks, P., Eden, J., Kernick, D., Murdoch, W., Lumley, L., Patel, R. P., Wong, S. W., Saigol, M., Ladha, K., Douglas, K., Cumberlidge, D. F., Bradshaw, C., Van Zon, G., Jones, K. P., Thomas, M. J., Watson, E., Sarai, B., Ahmad, N., Willcock, W., Cairns, J., Sathananthan, S., de Kare-Silver, N., Gilliland, A., Strieder, E., Howitt, A., Vishwanathan, B., Bird, N., Gray, D., Clark, M., Bisatt, J., Litchfield, J., Fisher, E., Fooks, T., Kelsall, A. R., Alborough, E., Wakeling, J., Parfitt, M., Milne, K., Rogers, S., Priyadharshan, R., Oliver, J. L., Davies, E., Abushal, S., Jacobs, M., Hutton, C., Walls, N. I., Thompson, R., Chigbo, C., Zaidi, S. M. A., Howard, M., Butter, K. C., Barrow, S., Little, H., Haq, I. U., Gibbons, L., Glencross, S., Mcleod, A. J., Poland, K., Mulholland, C., Warke, A., Conn, P., Burns, G., Smith, R. N., Lowe, S., Kamath, R., Dau, H. S., Webster, J., Hodgins, I., Vercoe, S., Roome, P. C., Pinnock, H., Patel, J. R. A., Ali, A., Hart, N., Davies, R., Stuart, E., Neden, C. A., Danielsen, M., Heath, R., Sharma, P., Galloway, S., Hawkins, C., Oliver, R., Aylward, M., Mannion, S., Braddick, M., Edwards, D., Rothwell, A. C., Sabir, A., Choudhary, F., Khalaque, S., Wilson, A., Peters, S., Coulson, W., Roberts, N., Heer, A., Coates, S., Ward, B., Jackson, D., Walton, S., Shepherd, D., Sterry, M., Wong, T., Boon, M., Bunney, R., Haria-Shah, R., Baron, R. T., Davies, S., Schatzberger, T., Hargreaves, N., Stephenson, T., Choi, H., Batson, R., Lucraft, L., Myhill, T., Estifano, S., Geatch, D., Wilkinson, J., Veale, R., Forshaw, K., Davies, T., Zaman, K., Vinson, P., Liley, C., Bandrapalli, M., Mcginty, P., Wastling, R., Mceleny, P., Beattie, A., Cooke, P., Wong, M., Gunasegaram, J., Pugsley, M., Ahmad, S., A'Court, C., Ayers, J., Bennett, J., Cartwright, S., Dobson, S., Dooldeniya, C., Flynn, A., Fox, R., Goram, J., Halpin, A., Hay, A., Jacobs, P., Jeffers, L., Lomax, L., Munro, I., Muvva, R., Nadaph, M., Powell, K., Randfield, S., Redpath, D., Reed, R., Rickenbach, M., Rogers, G., Saunders, P. B., Seamark, C., Shewring, J., Simmons, P., Simper, H., Stoddart, H., Sword, A., Thomas, N., Thomson, A., Gibbs, H., Blenkhorn, A., Singh, B., Van Gaal, W., Abhayaratna, W., Lehman, R., Roberts-Thomson, P., Kilian, J., Coulshed, D., Catanchin, A., Colquhoun, D., Kiat, H., Eccleston, D., French, J., Zimmett, L., Ayres, B., Phan, T., Blombery, P., Crimmins, D., O'Donnell, D., Choi, A., Astridge, P., Arstall, M., Jepson, N., Binnekamp, M., Lee, A., Rogers, J., Starmer, G., Carroll, P., Faunt, J., Aggarwala, A., Barry, L., Batta, C., Beveridge, R., Black, A., Bonner, M., Boys, J., Buckley, E., Campo, M., Carlton, L., Connelly, A., Conway, B., Cresp, D., Dimitri, H., Dixon, S., Dolman, M., Duroux, M., Eskandari, M., Eslick, R., Ferreira-Jardim, A., Fetahovic, T., Fitzpatrick, D., Geraghty, R., Gibbs, J., Grabek, T., Modi, M. H., Hayes, K., Hegde, M. P., Hesketh, L., Hoffmann, B., Jacobson, B., Johnson, K., Juergens, C., Kassam, I., Lawlor, V., Lehman, M., Lehman, S., Leung, D., Mackay, S., Mackenzie, M., Mccarthy, C., Mcintosh, C., Mckeon, L., Morrison, H., Mussap, C., Myers, J. -D., Nagalingam, V., Oldfield, G., O'May, V., Palmer, J., Parsons, L., Patching, K., Patching, T., Paul, V., Plotz, M., Preston, S., Rashad, H., Ratcliffe, M., Raynes, S., Rose, J., Sanders, L., Seremetkoska, M., Setio, H., Shone, S., Shrestha, P., Singh, C., Singleton, C., Stoyanov, N., Sutcliffe, S., Swaraj, K., Tarrant, J., Thompson, S., Tsay, I. M., Vorster, M., Waldman, A., Wallis, L., Wilford, E., Wong, K., Connolly, S. J., Spyropoulos, A., Eikelboom, J., Luton, R., Gupta, M., Pandey, A. S., Cheung, S., Leader, R., Beaudry, P., Ayala-Paredes, F., Berlingieri, J., Heath, J., Poirier, G., Du Preez, M., Nadeau, R., Dresser, G., Dhillon, R., Hruczkowski, T., Schweitzer, B., Coutu, B., Angaran, P., Macdonald, P., Vizel, S., Fikry, S., Parkash, R., Lavoie, A., Cha, J., Ramjattan, B., Bonet, J., Ahmad, K., Aro, L., Aves, T., Beaudry, K., Bergeron, C., Bigcanoe, J., Bignell, N., Breakwell, L., Burke, E., Carroll, L., Clarke, B., Cleveland, T., Daheb, S., Dehghani, P., Denis, I., Djaidani, Z., Dorian, P., Douglass, S., Dunnigan, J., Ewert, A., Farquhar, D., Fearon, A., Ferleyko, L., Fournier, D., Fox, B., Grenier, M. -C., Gulliver, W., Haveman, K., Hines, C., Hines, K., Jackson, A. M., Jean, C., Jethoo, G., Kahlon, R., Kelly, S., Kim, R., Korley, V., Kornder, J., Kwan, L., Largy, J., Lewis, C., Lewis, S., Mangat, I., Moor, R., Navratil, J., Neas, I., Otis, J., Otis, R., Pandey, M., Petrie, F., Pinter, A., Raines, M., Roberts, P., Robinson, M., Sas, G., Schulman, S., Snell, L., Spearson, S., Stevenson, J., Trahey, T., Wong, S., Wright, D., Ragy, H., Abd El-Aziz, A., Abou Seif, S. K., El Din, M. G., El Etriby, S., Elbahry, A., El-Etreby, A., Elkhadem, M., Katta, A., Khairy, T., Mowafy, A., Nawar, M., Ohanissian, A., Reda, A., Reda, M., Salem, H., Sami, N., Samir, S., Setiha, M., Sobhy, M., Soliman, A., Taha, N., Tawfik, M., Zaatout, E., Kettles, D., Bayat, J., Siebert, H., Horak, A., Kelfkens, Y., Garda, R., Pillay, T., Guerra, M., van Zyl, L., Theron, H., Murray, A., Louw, R., Greyling, D., Mntla, P., Ueckermann, V., Loghdey, R., Ismail, S., Ahmed, F., Engelbrecht, J., Ramdass, A., Maharajh, S., Oosthuysen, W., Angel, G., Bester, C., Booysen, M., Boshoff, C., Cannon, C., Cassimjee, S., Chami, C., Conway, G., Davids, A., de Meyer, L., Du Plessis, G., Ellis, T., Henley, L., Karsten, M., Loyd, E., Marks, J., Mavhusa, L., Mostert, M., Page, A., Rikhotso, L., Salie, M., Sasto, J., Shaik, F., Skein, A., Smith, L., Tarr, G., Tau, T., van Zyl, F., Al Mahmeed, W., Yousef, G., Agrawal, A., Nathani, M., Ibrahim, M., Esheiba, E. M., Singh, R., Naguib, A., Abu-Mahfouz, M., Al Omairi, M., Al Naeemi, A., Maruthanayagam, R., Bazargani, N., Wassef, A., Gupta, R., Khan, M., Subbaraman, B., Abdul, A., Al Mulla, A., El Bardisy, S., Haridas, P., Jadhav, S., Magdaluyo, K., Makdad, M., Maqsood, I., Mohamed, R., Sharma, N., Sharma, R., Thanzeel, M., Goldhaber, S. Z., Canosa, R., Rama, P., Blumberg, E., Garcia, J., Mullen, P., Wilson, V., Quick, A., Ferrick, K., Kutayli, W. M., Cox, M., Franco, M., Falkowski, S., Mendelson, R., Williams, M., Miller, S., Beach, S., Alfieri, A., Gutowski, T., Haque, I., Reddy, R., Ahmed, W., Delafontaine, P., Diercks, D., Theodoro, D., Remmel, K., Alberts, M., Ison, R., Noveck, H., Duffy, P., Pitta, S., Nishijima, D., Treasure, C., Asafu-Adjaye, N., Ball, K., Bartlett, M., Bentley, M., Bowers, S., Brown, A., Browne, A., Cameron-Watts, J., Canova, M., Cassidy, D., Cervellione, K., Congal, S., Depauw, J., Dickerson, A., Eley, M., Evans, L., Felpel, S., Ferdinand, K., Fielder, D., Gentry, P., Haideri, A., Hakimi, F., Harbour, T., Hartranft, E., Hawkins, B., Headlee, M., Henson, L., Herrick, C., Hicks, T., Jasinski, S., Jones, A., Jones, L., Jones, P., Karl, S., Keeling, M., Kerr, J., Knowles, P., Langdon, J., Lay, M., Lee, J. A., Lincoln, T., Malone, E., Merliss, A., Merritt, D., Minardo, J., Mooso, B., Orosco, C., Palumbo, V., Parker, M., Parrott, T., Paserchia, S., Pearl, G., Peterson, J., Pickelsimer, N., Purcell, T., Raynor, J., Raziano, S., Richard, C., Richardson, T., Robertson, C., Sage, A., Sanghera, T., Shaw, P., Shoemaker, J., Smith, K., Stephanie, B., Thatcher, A., Theobald, H., Thompson, N., Treasure, L., Tripti, T., Verdi, C., and Worthy, V.
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Registrie ,Male ,Time Factors ,Anticoagulant therapy, Arrhythmia, Atrial fibrillation, GARFIELD-AF ,030204 cardiovascular system & hematology ,Electrocardiography ,0302 clinical medicine ,Risk Factors ,Anticoagulant therapy ,Arrhythmia ,Atrial fibrillation ,GARFIELD-AF ,Aged ,Atrial Fibrillation ,Female ,Follow-Up Studies ,Humans ,Incidence ,India ,Prevalence ,Prognosis ,Prospective Studies ,Survival Rate ,Thromboembolism ,Registries ,Risk Assessment ,Medicine ,030212 general & internal medicine ,Prospective cohort study ,Incidence (epidemiology) ,cardiovascular system ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Risk assessment ,Human ,medicine.medical_specialty ,Time Factor ,RD1-811 ,Prognosi ,Follow-Up Studie ,03 medical and health sciences ,Internal medicine ,Diseases of the circulatory (Cardiovascular) system ,cardiovascular diseases ,Risk factor ,Survival rate ,Fibrillation ,business.industry ,Risk Factor ,ta3121 ,medicine.disease ,Clinical trial ,Prospective Studie ,RC666-701 ,Surgery ,Clinical and Preventive Cardiology ,business - Abstract
Background: The Global Anticoagulant Registry in the FIELD–Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. Methods and results: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012–2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P
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- 2018
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4. A062 A Web-based Registry / Clinical Reporting Tool for Cardiac Magnetic Resonance Imaging linked to ANZACS-QI: Development and Implementation
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J.L. Looi, Andrew C. Kerr, R. Gabriel, and P. Weeks
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Cardiac magnetic resonance imaging ,Web application ,Medicine ,Medical physics ,Cardiology and Cardiovascular Medicine ,business - Published
- 2020
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5. A060 Māori and Pacific Patients With Non-Ischaemic Cardiomyopathy, Undergoing Cardiac Magnetic Resonance Imaging Have Worse Clinical Outcomes than European Patients
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A. Lydon, J. Voss, M. Lund, R. Davis, J.L. Looi, P. Weeks, R. Gabriel, J. Chieng, T. Sutton, T. Pasley, and Andrew C. Kerr
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Cardiac magnetic resonance imaging ,Internal medicine ,Cardiology ,Medicine ,Non ischemic ,Cardiology and Cardiovascular Medicine ,business - Published
- 2020
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6. Music Interventions in Patients During Coronary Angiographic Procedures: A Randomized Controlled Study of the Effect on Patients' Anxiety and Well-Being
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Birgit P. Weeks and Ulrica Nilsson
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Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Psychological intervention ,Cardiac catheterization lab ,Anxiety ,Coronary Angiography ,law.invention ,Randomized controlled trial ,law ,Humans ,Medicine ,In patient ,Angioplasty, Balloon, Coronary ,Music Therapy ,Aged ,Cardiac catheterization ,Aged, 80 and over ,Advanced and Specialized Nursing ,Relaxation (psychology) ,business.industry ,fungi ,food and beverages ,Middle Aged ,Medical–Surgical Nursing ,Treatment Outcome ,Cardiovascular Diseases ,Patient Satisfaction ,Well-being ,Physical therapy ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Abstract
A cardiac catheterization laboratory can be a frightening environment and music can be a supportive source of environmental sound that stimulates and maintains relaxation.To test the effects of patient focused music versus loudspeaker music versus standard sound on patient's experiences of anxiety and well-being during coronary angiographic procedures.A prospective, randomized, controlled trial of 98 subjects undergoing elective coronary angiogram and/or percutaneous coronary intervention. The subjects were randomly allocated to three different groups of sound environments: a control group (the usual sound environment), a patient focused music group (audio pillow) or to a loudspeaker music group.Anxiety decreased significantly and well-being increased significantly in the two music groups compared to the control group. There was a significantly more positive impression of the sound environment in the patient focused music group compared to the two other groups.This study showed that the use of a specially designed music reduced anxiety and increased well-being in patients during coronary angiographic procedures. However, patient focused music seemed to be more preferable. The sound environment was rated more positively by the subjects listening to music via audio pillow. The music delivered via loudspeakers seemed to distract the staff during the examination at the cardiac catheterization laboratory.
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- 2011
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7. Is the Rate of Congenital Heart Defects Detected by Fetal Echocardiography Among Pregnancies Conceived by In Vitro Fertilization Really Increased?
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Bevin P. Weeks, Alan H. Friedman, Katherine H. Campbell, Mert Ozan Bahtiyar, Joshua A. Copel, Antonette T. Dulay, and Olivera Kontic-Vucinic
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Heart Defects, Congenital ,medicine.medical_specialty ,medicine.medical_treatment ,Population ,Fertilization in Vitro ,030204 cardiovascular system & hematology ,Ultrasonography, Prenatal ,Intracytoplasmic sperm injection ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Risk Factors ,Internal medicine ,Diseases in Twins ,Prevalence ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,education ,reproductive and urinary physiology ,education.field_of_study ,030219 obstetrics & reproductive medicine ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,business.industry ,Obstetrics ,Pregnancy Outcome ,Case-control study ,Odds ratio ,medicine.disease ,United States ,3. Good health ,Echocardiography ,Case-Control Studies ,Cardiology ,Population study ,Gestation ,Female ,Pregnancy, Multiple ,business ,Fetal echocardiography - Abstract
OBJECTIVE We investigated the prenatal prevalence of congenital heart defects (CHDs) among in vitro fertilization (IVF) pregnancies at a referral program in the United States. METHODS Study patients were referred for fetal echocardiography between April 1, 2006, and May 1, 2009, due to IVF. An IVF pregnancy was defined as a patient who conceived with IVF with or without intracytoplasmic sperm injection. Congenital heart defect odds relative to historical data were calculated by standard methods. P < .05 was considered statistically significant. RESULTS During the study period, we performed fetal echocardiography on 749 consecutive IVF pregnancies. Overall, the frequency of CHDs was 1.1% (95% confidence interval, 0.3%-1.8%) per pregnancy. Compared to earlier historical population data, IVF pregnancies had a significantly higher risk of CHDs (odds ratios, 7.3 [3.6-14.7] and 2.9 [1.4-5.9], respectively). However, compared to more contemporary population data, there was no difference in the CHD risk between IVF gestations and naturally conceived pregnancies. Further analysis indicated that IVF twin pregnancies were as much as 12.5 (4.6-33.5) times as likely to have CHDs compared to a general population. CONCLUSIONS In this study population, the frequency of CHDs in IVF pregnancies was higher than early historical population data; however, it was similar to that of a more contemporary general population. Further analysis showed that this increase was mainly driven by IVF twin gestations. Previous reports of increased CHD risk in pregnancies conceived via IVF may have been due, in part, to an increased frequency of higher-order pregnancies seen among these patients.
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- 2010
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8. The use of botulinum toxin type-B in the treatment of patients who have become unresponsive to botulinum toxin type-A - initial experiences
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P. Weeks, B. Roberts, J. Whitaker, L. Kidd, S. Stark, D. Best, and M. P. Barnes
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Male ,medicine.medical_specialty ,Botulinum Toxins ,Blepharospasm ,Enzyme-Linked Immunosorbent Assay ,medicine.disease_cause ,Antibodies ,Humans ,Medicine ,Cervical dystonia ,Spasticity ,Botulinum Toxins, Type A ,business.industry ,Toxin ,Drug Tolerance ,Middle Aged ,Focal dystonia ,medicine.disease ,Botulinum toxin ,Surgery ,Dystonia ,Treatment Outcome ,Neuromuscular Agents ,Neurology ,Muscle Spasticity ,Anesthesia ,Female ,Neurology (clinical) ,medicine.symptom ,business ,Botulinum toxin type ,Hemifacial spasm ,medicine.drug - Abstract
The increasing use of botulinum toxin type-A, especially for focal dystonia and spasticity has highlighted the issue of secondary non-responsiveness. Within the last few years botulinum toxin type-B (Myobloc®/Neurobloc®) has become commercially available as an alternative to type-A. This paper discusses our initial experience of botulinum toxin type-B in a total of 63 individuals who attended our botulinum clinic. Thirty-six patients had cervical dystonia and a secondary non-response to type-A toxin. Thirteen of these patients (36%) had a reasonable clinical response to Neurobloc® and continue to have injections. The other 23 patients either had no response, or a poor response, or had unacceptable side effects and ceased treatment. A small number of people with blepharospasm, hemifacial spasm and foot dystonia also had a disappointing response to injection. Twenty patients with spasticity were also type-A resistant. Seven of these show some continuing response to type-B, without unacceptable side effects. These findings demonstrate that botulinum toxin type-B has a place in the management of patients who have become non-responsive to type-A, but overall the responses to type-B toxin were disappointing.
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- 2005
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9. A rapid live-cell ELISA for characterizing antibodies against cell surface antigens of Chlamydomonas reinhardtii and its use in isolating algae from natural environments with related cell wall components
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Wenzhi Jiang, George A. Oyler, Donald P. Weeks, Bradley J. S. C. Olson, Julian N. Rosenberg, and Sarah Cossey
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Live-cell ELISA ,Algae ,Recombinant Fusion Proteins ,Cell ,Chlorophyceae ,Chlamydomonas reinhardtii ,VHH ,Enzyme-Linked Immunosorbent Assay ,Plant Science ,Environment ,Camelid antibodies ,Cell wall ,Antibody Specificity ,Cell Wall ,Genes, Reporter ,Botany ,Escherichia coli ,Microalgae ,medicine ,Animals ,Phylogeny ,Bioprospecting ,Cell wall conservation ,biology ,Methodology Article ,Chlamydomonas ,Antigens, Plant ,Single-Domain Antibodies ,Flow Cytometry ,biology.organism_classification ,medicine.anatomical_structure ,Biochemistry ,Antigens, Surface ,Nanobodies ,Cell walls ,Camelids, New World ,Bacteria ,Archaea - Abstract
Background Cell walls are essential for most bacteria, archaea, fungi, algae and land plants to provide shape, structural integrity and protection from numerous biotic and abiotic environmental factors. In the case of eukaryotic algae, relatively little is known of the composition, structure or mechanisms of assembly of cell walls in individual species or between species and how these differences enable algae to inhabit a great diversity of environments. In this paper we describe the use of camelid antibody fragments (VHHs) and a streamlined ELISA assay as powerful new tools for obtaining mono-specific reagents for detecting individual algal cell wall components and for isolating algae that share a particular cell surface component. Results To develop new microalgal bioprospecting tools to aid in the search of environmental samples for algae that share similar cell wall and cell surface components, we have produced single-chain camelid antibodies raised against cell surface components of the single-cell alga, Chlamydomonas reinhardtii. We have cloned the variable-region domains (VHHs) from the camelid heavy-chain-only antibodies and overproduced tagged versions of these monoclonal-like antibodies in E. coli. Using these VHHs, we have developed an accurate, facile, low cost ELISA that uses live cells as a source of antigens in their native conformation and that requires less than 90 minutes to perform. This ELISA technique was demonstrated to be as accurate as standard ELISAs that employ proteins from cell lysates and that generally require >24 hours to complete. Among the cloned VHHs, VHH B11, exhibited the highest affinity (EC50
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- 2014
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10. Nutritional condition and fertility of white-tailed deer (Odocoileus virginianus) from areas with contrasting histories of hunting
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Robert K. Swihart, Harmon P Weeks, Anthony J. DeNicola, and Andrea L Easter-Pilcher
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Pregnancy ,White (horse) ,biology ,Ecology ,media_common.quotation_subject ,Fertility ,Odocoileus ,Fecundity ,medicine.disease ,biology.organism_classification ,Population density ,medicine ,Herd ,Animal Science and Zoology ,Reproduction ,Ecology, Evolution, Behavior and Systematics ,Demography ,media_common - Abstract
We evaluated the nutritional condition and pregnancy rates of 58 female white-tailed deer (Odocoileus virginianus) from five areas in Indiana, U.S.A., with differing herd densities and histories of hunting. We found significant differences among sites for five external measures of body size, six physiological indicators of nutritional restriction, and five internal postmortem measures of body size and fat reserves. Multivariate assessment of nutritional condition provided an appreciable increase in classification of deer to their site of origin relative to reliance on any single variable. In addition, postmortem variables and physiological indicators contributed significantly to our ability to correctly classify deer. Nearly all measures of nutrition or stress indicated that deer from sites hunted annually were in better nutritional condition than deer from sites hunted only once in the past several decades. Fertility rates differed significantly among the sites and varied inversely with density. Comparison with other studies within the central and northern hardwood regions suggests that weak density dependence in fertility rates occurs among adult does, although substantial reproduction still occurs when densities exceed 50 deer/km2. Density effects become progressively stronger for first-year and yearling females, with reproduction in the former age group virtually ceasing when densities exceed 30 deer/km2.
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- 1998
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11. Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option
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Aleks, James R Ferrell, Judith A. Fulton, Steven P Weeks, Valery A Gostev, Christopher J. Woolverton, r S Galov, and Bruce A Banks
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Glow discharge ,medicine.diagnostic_test ,Chemistry ,Radiochemistry ,chemistry.chemical_element ,Plasma ,Sterilization (microbiology) ,Nonthermal plasma ,Plasma filtration ,Oxygen ,Environmental chemistry ,Spectrophotometry ,medicine ,Ultraviolet radiation - Abstract
The glow discharge from a novel spark-based nonthermal plasma generator is described and characterized using spectrophotometry, radiometry and gas detectors. Spectral information identified ultraviolet radiation within the plasma discharge. High levels of reactive oxygen and nitrogenous species were also detected within the analyzed plasma. The plasma discharge is found to have a significant germicidal effect on Gram-positive bacteria, independent of temperature increase. The ultraviolet radiation and ionized gaseous species are found to contribute to this observed effect, synergistically. Plasma filtration reduced germicidal survivability significantly. The liquid environment in which bacterial cells were exposed to the nonthermal plasma also was important, when determining the efficacy of nonthermal plasma, as a germicidal agent. Staphylococcus aureus cultures exposed to nonthermal plasma for two minutes within low nutrient sources, such as water or phosphate-buffered saline, had very low survival. Cultures exposed to the same plasma interval in nutrient rich liquids tolerated the plasma exposure better, and survived at higher numbers. This plasma discharge is highly bactericidal, due to the high concentrations of known antimicrobial agents, but its effectiveness is subject to a synergistic effect, that depends upon the surrounding environment.
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- 2013
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12. Understanding the episode of care for transplant patients
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P Weeks and I Blais
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medicine.medical_specialty ,Evidence-based practice ,business.industry ,Health Policy ,Public health ,Nursing research ,lcsh:Public aspects of medicine ,lcsh:RA1-1270 ,Health informatics ,Health administration ,Nursing ,Meeting Abstract ,Health care ,Medicine ,business ,Lump sum ,health care economics and organizations ,Cost database - Abstract
Funders and policy makers are looking for ways to manage increasing healthcare costs and provide funding for entire episodes of care that extend beyond a single stay or visit at a healthcare institution. In Ontario hospital funding is based on global funding, which is a lump sum distributed annually to hospitals. A small percentage of funding in Ontario is based on volume and fixed fees for selected procedures. In a departure from the current funding policies, the Ontario Ministry of Health and Long-Term Care (MoHLTC) is formulating a strategy to increase patient based funding. This initiative is known as Patient based Payment (PbP). To execute this change in funding policy, the MoHLTC is extensively using patient level cost data to develop strategy and funding models. To gather evidence based information, the MoHTLC has funded a case-costing project to assist 48 facilities produce patient level cost data. The current information systems and reporting standards in Canada are designed to record patient-care information by patient type during a single stay or visit in one institution. Although patients may receive health care from multiple healthcare providers, patient information is not linked or accessible by the multiple healthcare providers. The limitations of the current information systems have resulted in patient data that is difficult to access even within a single healthcare facility. The single episode of care (one stay or visit) is not representative of the healthcare needed, or the entire cost of care related to a condition. This is particularly true for patients living with chronic diseases, or patients who have received complex medical procedures such as transplants and, ultimately, the healthcare services received throughout a patient’s life. The transplant process is complex and can be divided into four phases: pre-transplant, assessment and waiting period; donor procurement; peri-operative; and post-discharge phase. Pre- and post-transplant healthcare services are not included in the procedure based funding and may be provided by multiple healthcare providers. Moreover, post-transplant care has increased as a result of improved survival rate resulting in continued healthcare. In response, we propose to develop a linking of patient data describing complete care for all activities related to transplant. We suggest that a new model for episodic care is needed that provides funding for an entire episode of care. This will result in the proper incentives for improved care between providers. In this paper, we identify challenges and opportunities for this type of work in the future.
- Published
- 2011
13. Modified Starnes procedure in a neonate with severe tricuspid regurgitation
- Author
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Bevin P. Weeks, Toshiharu Shinoka, Gary S. Kopf, Nicholas P. Pietris, Ashok Muralidaran, Vaughn A. Starnes, and Richard W. Kim
- Subjects
Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Decompression ,medicine.medical_treatment ,Heart Ventricles ,Regurgitation (circulation) ,Pulmonary Artery ,Fontan Procedure ,Prosthesis Design ,Heart Septal Defects, Atrial ,Fontan procedure ,Internal medicine ,Ductus arteriosus ,medicine ,Pericardium ,Humans ,Cardiac Surgical Procedures ,Ductus Arteriosus, Patent ,Bioprosthesis ,Heart septal defect ,Pericardial patch ,business.industry ,Infant, Newborn ,medicine.disease ,Decompression, Surgical ,Tricuspid Valve Insufficiency ,Surgery ,medicine.anatomical_structure ,Ventricle ,Pulmonary Atresia ,Heart Valve Prosthesis ,Cardiology ,Female ,Tricuspid Valve ,Cardiology and Cardiovascular Medicine ,business - Abstract
We report a modification of the Starnes technique for palliating severe tricuspid regurgitation associated with a dysplastic right ventricle in a neonate, using a fenestrated pericardial patch allowing for unidirectional flow. The patient eventually underwent a successful Glenn shunt construction with a persistent reduction in right ventricle size at 1 year follow-up.
- Published
- 2011
14. Fetal Cardiac Arrhythmias
- Author
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Mert Ozan Bahtiyar, Joshua A. Copel, and Bevin P. Weeks
- Subjects
Fetus ,medicine.medical_specialty ,business.industry ,Internal medicine ,Cardiology ,medicine ,business - Published
- 2011
- Full Text
- View/download PDF
15. Prenatal course of isolated muscular ventricular septal defects diagnosed only by color Doppler sonography: single-institution experience
- Author
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Bevin P. Weeks, Mert Ozan Bahtiyar, Joshua A. Copel, Alan H. Friedman, and Antonette T. Dulay
- Subjects
Heart Septal Defects, Ventricular ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine.medical_specialty ,Twins ,Aneuploidy ,Gestational Age ,Risk Assessment ,Ultrasonography, Prenatal ,Cohort Studies ,Fetal Development ,Fetal Heart ,Pregnancy ,Nuchal Translucency Measurement ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Prospective Studies ,Ultrasonography, Doppler, Color ,Prospective cohort study ,Increased nuchal translucency ,Heart septal defect ,Triplets ,Radiological and Ultrasound Technology ,business.industry ,Infant, Newborn ,Pregnancy Outcome ,Gestational age ,medicine.disease ,Surgery ,Fetal Diseases ,Echocardiography ,Female ,Radiology ,Down Syndrome ,Trisomy ,business ,Follow-Up Studies - Abstract
Objective. Counseling patients with an isolated ventricular septal defect (i-VSD) is clinically important because with high-resolution ultrasound equipment, more small muscular VSDs are now being diagnosed. The prevalence of these lesions is not yet completely described, and the frequency with which muscular VSDs resolve in utero has also not been extensively reported. Methods. We investigated the perinatal course of isolated muscular VSDs diagnosed only on color Doppler examinations and followed between January 1, 2005, and December 31, 2006. A complete evaluation of the fetal heart was performed by gray scale, spectral Doppler, and color Doppler examinations. Results. We performed a total of 2583 fetal echocardiographic examinations on 2410 fetuses during 2318 pregnancies. The study group included 78 twin gestations (3.4%) and 7 triplet gestations (0.3%). There were 16 fetuses with an i-VSD (6.6/1000 fetuses) within the study group. The mean gestational age ± SD at diagnosis was 23.5 ± 4.3 weeks. Two of the i-VSDs (12.5%) spontaneously resolved prenatally. One fetus with an i-VSD had trisomy 21 and also had increased nuchal translucency in the first trimester. One i-VSD was diagnosed among 22 fetuses with trisomy 21 examined during the study period. Conclusions. An i-VSD is a common congenital heart defect. Prenatal resolution of i-VSDs is less frequent than reported in the literature. A larger cohort is needed to provide a better risk estimate for aneuploidy in the presence of an i-VSD. Key words: congenital heart defect; isolated; ventricular septal defect.
- Published
- 2008
16. Prevalence of congenital heart defects in monochorionic/diamniotic twin gestations: a systematic literature review
- Author
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Bevin P. Weeks, Joshua A. Copel, Alan H. Friedman, Antonette T. Dulay, and Mert Ozan Bahtiyar
- Subjects
Heart Defects, Congenital ,medicine.medical_specialty ,Internationality ,Population ,Risk Assessment ,Atrial septal defects ,Pregnancy ,Risk Factors ,medicine ,Diseases in Twins ,Prevalence ,Twins, Dizygotic ,Humans ,Radiology, Nuclear Medicine and imaging ,Risk factor ,education ,Twin Pregnancy ,education.field_of_study ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,Obstetrics ,business.industry ,Twins, Monozygotic ,medicine.disease ,Stenosis ,Relative risk ,Gestation ,Female ,business ,Fetal echocardiography - Abstract
Objective. Congenital heart defects (CHDs) affect approximately 0.5% of all neonates. Recent literature points to a possible increase in the CHD prevalence among monochorionic/diamniotic (MC/DA) twin gestations. We hypothesized that MC/DA twin pregnancy is a risk factor for CHD. Methods. A systematic review of all published English literature was conducted on MEDLINE (Ovid and PubMed) from January 2000 through April 2007 using the medical subject heading terms "congenital heart defect" and "monozygotic twins." Four observational studies were included in the final analysis. Published historical data were used for the population background risk of CHD. Relative risk (RR) estimates with 95% confidence intervals (CIs) were calculated by fixed and random effect models. Results. We included a total of 40 fetuses with CHDs among 830 fetuses from MC/DA twin gestations. Compared with the population, CHDs were significantly more prevalent in MC/DA twins regardless of the presence of twin-twin transfusion syndrome (TTTS) (RR, 9.18; 95% Cl, 5.51-15.29; P
- Published
- 2007
17. Outcomes after a Ladd procedure for intestinal malrotation with heterotaxia
- Author
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Robert J. Touloukian, Martina Brueckner, Bevin P. Weeks, and David B. Tashjian
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Cardiovascular Abnormalities ,Comorbidity ,Asymptomatic ,medicine ,Humans ,Digestive System Surgical Procedures ,business.industry ,General surgery ,Incidence (epidemiology) ,Medical record ,Infant, Newborn ,Midgut volvulus ,Infant ,General Medicine ,Bowel resection ,medicine.disease ,Surgery ,Bowel obstruction ,Intestinal Diseases ,Treatment Outcome ,Intestinal malrotation ,Pediatrics, Perinatology and Child Health ,Female ,medicine.symptom ,business ,Digestive System Abnormalities ,Follow-Up Studies - Abstract
Purpose Intestinal rotation abnormalities and complex congenital heart disease associated with heterotaxia coexist. Despite the risk for midgut volvulus, performing a Ladd procedure for asymptomatic malrotation with heterotaxia remains to be controversial because the presumed risk for postoperative complications is thought to exceed the benefits of the operation. The purpose of this study was to review the incidence of complications after a Ladd procedure in asymptomatic patients with heterotaxia to guide recommendations for this patient population. Methods The medical records of all patients with heterotaxia who underwent a Ladd procedure for asymptomatic malrotation between 1984 and 2004 were reviewed. Type of cardiac disease, postoperative complications, and survival were recorded. Results Twenty-two patients (9 boys and 13 girls) with heterotaxia underwent an elective Ladd procedure after their medical stabilization or surgical correction or palliation of their cardiac anomaly. Of these patients, 19 were younger than 1 month at the time of the operation. The remaining 3 patients underwent the operation when they were between 2 and 5 months old. Three of the 22 patients (14%) developed postoperative intestinal obstruction: lysis of adhesions was performed in 1 patient; another patient required a staged bowel resection for a closed loop obstruction; and yet another patient had recurrent midgut volvulus 4 years after an incomplete initial Ladd procedure. All patients survived the initial and secondary procedures. Four deaths, all more than 1 month after the surgery, occurred as sequelae of the underlying cardiac anomaly. Length of follow-up ranged from 1 to 17 years. Conclusions We report on a 14% risk of postoperative bowel obstruction after an elective Ladd procedure, as compared with a small but significant incidence of midgut volvulus in patients with malrotation in the setting of complex congenital heart disease. Our results support the conclusion that an elective Ladd procedure at a time of relative cardiac stability for selected patients with heterotaxia has an acceptably low morbidity and should be considered to prevent midgut volvulus.
- Published
- 2007
18. P04.01: The changing face of fetal echocardiography
- Author
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Ozan M. Bahtiyar, Bevin P. Weeks, Joshua A. Copel, and Charles S. Kleinman
- Subjects
medicine.medical_specialty ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,business.industry ,Obstetrics and Gynecology ,Face (sociological concept) ,General Medicine ,Reproductive Medicine ,Internal medicine ,medicine ,Cardiology ,Radiology, Nuclear Medicine and imaging ,business ,Fetal echocardiography - Published
- 2013
- Full Text
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19. Training pediatric residents to evaluate congenital heart disease in the current era
- Author
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Alan H. Friedman and Bevin P. Weeks
- Subjects
Heart Defects, Congenital ,Medical education ,Pediatrics ,medicine.medical_specialty ,Students, Medical ,Heart disease ,business.industry ,education ,Medical school ,Cardiology ,Internship and Residency ,medicine.disease ,Training (civil) ,Pediatrics, Perinatology and Child Health ,medicine ,Medical training ,Humans ,business ,Pediatric cardiology - Abstract
Working with young pediatricians to create an exciting educational environment in which to learn cardiology remains a challenge for all of us. Numerous forces impact our efforts, making the process of training residents to evaluate and treat congenital heart disease in the current era a dynamic and, at times, difficult endeavor. This article considers the changes that have occurred in the medical school graduate who chooses pediatrics and subsequently pediatric cardiology as a career; explores the changes in the graduate medical training guidelines, requirements, and restrictions that have been put into place within the last several years; and discusses the seemingly constant advances in scientific understanding and technology that shape our field and move us forward as a discipline.
- Published
- 2004
20. Molecular analysis of the acetolactate synthase gene of Chlamydomonas reinhardtii and development of a genetically engineered gene as a dominant selectable marker for genetic transformation
- Author
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Donald P. Weeks, Joy Kovar, Roel P. Funke, and Jun Zhang
- Subjects
Genetic Markers ,DNA, Complementary ,Mutant ,Molecular Sequence Data ,Drug Resistance ,Mutagenesis (molecular biology technique) ,Chlamydomonas reinhardtii ,Plant Science ,Biology ,medicine.disease_cause ,Transformation, Genetic ,Genetics ,medicine ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Gene ,Selectable marker ,Acetolactate synthase ,Mutation ,Dose-Response Relationship, Drug ,Organisms, Genetically Modified ,Sequence Homology, Amino Acid ,Herbicides ,Cell Biology ,biology.organism_classification ,Transformation (genetics) ,Acetolactate Synthase ,Sulfonylurea Compounds ,Biochemistry ,Amino Acid Substitution ,biology.protein - Abstract
Genomic and cDNA clones of the acetolactate synthase (ALS) gene of Chlamydomonas reinhardtii have been isolated from a mutant, c85-20 (Hartnett et al., 1987), that is resistant to high concentrations of sulfometuron methyl (SMM) and related sulfonylurea herbicides. Comparison of the ALS gene sequences from the wild-type and the SMM resistant (SMMr) strains revealed two amino acid differences in the mature enzyme, a lysine to threonine change at position 257 (K257T) and a leucine to valine change at position 294 (L294V). Transformation of wild-type C. reinhardtii with the mutant ALS gene produced no transformants with ability to grow in the presence of a minimum toxic concentration of SMM (3 microm). Substitution of the ALS promoter with the promoter of the C. reinhardtii Rubisco small subunit gene (RbcS2) permitted recovery of SMMr colonies. In vitro mutagenesis of the wild-type ALS gene to produce various combinations of mutations (K257T, L294V and W580L) indicated that the K257T mutation was necessary and sufficient to confer the SMMr phenotype. Optimum transformation rates were obtained with two constructs (pJK7 and pRP-ALS) in which all introns in the coding region were present. Rates of transformation with construct pJK7 were approximately 2.5 x 10-4 transformants/cell (i.e. one transformant for each of 4000 initial cells) using electroporation and 8.5 x 10-6 transformants/cell using the glass bead vortexing method. These results suggest that pJK7 and pRP-ALS can serve as important additional dominant selectable markers for the genetic transformation of C. reinhardtii.
- Published
- 2002
21. Intracellular carbonic anhydrase is essential to photosynthesis in Chlamydomonas reinhardtii at atmospheric levels of CO2. Demonstration via genomic complementation of the high-CO2-requiring mutant ca-1
- Author
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Donald P. Weeks, R. P. Funke, and Joy Kovar
- Subjects
Physiology ,Mutant ,Genes, Protozoan ,Molecular Sequence Data ,Chlamydomonas reinhardtii ,Plant Science ,medicine.disease_cause ,Transformation, Genetic ,Genetics ,medicine ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Photosynthesis ,Gene ,Carbonic Anhydrases ,Mutation ,biology ,Base Sequence ,Genetic Complementation Test ,Carbon Dioxide ,DNA, Protozoan ,biology.organism_classification ,Cosmids ,Molecular biology ,Complementation ,Transformation (genetics) ,genomic DNA ,Biochemistry ,Cosmid ,Research Article - Abstract
Genomic complementation of the high-CO2-requiring mutant ca-1–12-1C of Chlamydomonas reinhardtii was achieved by transformation with DNA pools from an indexed cosmid library of wild-type genomic DNA. Transformation of mutant cells with cosmid DNA from two microtiter plates in the library produced colonies that grew phototrophically at atmospheric CO2 levels. Transformations with cosmid DNA from each of the rows and files of the two plates pinpointed one well in each plate with a cosmid bearing the targeted gene. Sequencing of cosmid subclones revealed a gene encoding a recently identified C. reinhardtii chloroplast carbonic anhydrase (CAH3). Transformations with chimeric constructs combining different portions of the wild-type and mutant genes indicated the presence of a mutation in the 5[prime]-half of the gene. Comparison of mutant and wild-type gene sequences in this region revealed a G-to-A substitution in the mutant gene, which produced a nonsense codon. The data presented demonstrate that the carbonic anhydrase produced from the CAH3 gene is essential to the inorganic carbon-concentrating mechanism in C. reinhardtii and that genomic complementation can be a facile and efficient means for isolating genes associated with defects affecting photosynthesis and other physiological processes in this eukaryotic green alga.
- Published
- 1997
22. Electrogenicity of Na-coupled bile salt transport in isolated rat hepatocytes
- Author
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R. P. Weeks and Steven A. Weinman
- Subjects
Male ,Taurocholic Acid ,Physiology ,Sodium ,chemistry.chemical_element ,Cell Separation ,Bile Acids and Salts ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Chlorides ,Physiology (medical) ,medicine ,Animals ,Membrane potential ,Alanine ,Hepatology ,Gastroenterology ,Ursodeoxycholate ,Biological Transport ,Membrane transport ,Taurocholic acid ,Rats ,Electrophysiology ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Liver ,Hepatocyte ,Potassium ,Cotransporter ,Intracellular - Abstract
The importance of membrane voltage in uptake of bile salts into hepatocytes is not known. Electrogenicity of the primary bile salt transport process, Na-bile salt cotransport, has been difficult to determine because the large K and Cl conductances of the sinusoidal membrane (GK and GCl, respectively) obscure any transport associated currents. In the present study hepatocytes were treated to reduce these membrane conductances and electrogenic entry of taurocholate and glycocholate was demonstrated. Intracellular voltage and resistance changes resulting from bile salt transport were measured in hepatocytes in which GK and GCl were blocked by impalement with Na acetate microelectrodes and external exposure to quinine (400 microM). This increased the cell input resistance from 153 +/- 17 to 230 +/- 17 M omega (n = 14, P < 0.001). Under these conditions, exposure to 100 microM of taurocholate or glycocholate produced Na-dependent depolarizations of 3.0 +/- 0.5 and 4.2 +/- 0.8 mV, respectively. These correspond to transport currents of 13.9 and 7.6 pA/cell, which are comparable to those predicted from known [3H]taurocholate uptake rates if one positive charge enters the cell with each bile salt molecule. Although uptake of these two bile salts was electrogenic, this was not the case for all bile salts. Na-dependent transport of taurodehydrocholate, which occurs at similar rates to that for taurocholate, produced no voltage change. The unconjugated bile salts cholate and ursodeoxycholate also produced no measurable voltage or resistance changes. In conclusion, Na-dependent uptake of taurocholate and glycocholate is electrogenic, whereas uptake of taurodehydrocholate, ursodeoxycholate, and cholate is predominantly electroneutral.(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1993
23. 359: Prenatal diagnosis of congenital heart defects and 22q11 microdeletion
- Author
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Alan M. Friedman, Ling Li, Mert Ozan Bahtiyar, Bevin P. Weeks, and Joshua A. Copel
- Subjects
Pediatrics ,medicine.medical_specialty ,business.industry ,medicine ,Obstetrics and Gynecology ,Prenatal diagnosis ,22q11 microdeletion ,business - Published
- 2008
- Full Text
- View/download PDF
24. OP02.14: Fetuses with congenital heart defect have similar growth patterns
- Author
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Mert-Ozan Bahtiyar, Sun Jin Lee, Allan H. Friedman, Joshua A. Copel, Bevin P. Weeks, and Catalin S. Buhimschi
- Subjects
medicine.medical_specialty ,Fetus ,Reproductive Medicine ,Radiological and Ultrasound Technology ,business.industry ,Internal medicine ,Cardiology ,Obstetrics and Gynecology ,Medicine ,Radiology, Nuclear Medicine and imaging ,Heart defect ,General Medicine ,business - Published
- 2008
- Full Text
- View/download PDF
25. Sex- and Age-specific Patterns of Mineral Lick Use by White-tailed Deer (Odocoileus virginianus)
- Author
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Todd C. Atwood and Harmon P. Weeks
- Subjects
digestive, oral, and skin physiology ,Biology ,Odocoileus ,biology.organism_classification ,Age specific ,Antler ,White (mutation) ,medicine.anatomical_structure ,Animal science ,Lactation ,medicine ,Gestation ,Mineral lick ,Ecology, Evolution, Behavior and Systematics - Abstract
Little is known regarding the influence of sex- and age-specific factors on mineral lick use by white-tailed deer (Odocoileus virginianus). We recorded 1020 visits to mineral licks by deer between April and September, of which we could classify 922 by sex and age (yearling or adult). We observed a relationship between the frequency of mineral lick visitation and both sex and age of users. No relationship was found between visitation frequency and time interval of use. Adult females used licks more frequently than other sex/age classes. Visitation frequencies were similar for adult males and yearling males and females. We detected no relationship between lick sodium (Na) content and sex or age of visitors. Greater lick use by adult females may have reflected an increased need for Na associated with gestation and lactation. The majority of male visitors were yearlings, which require Na for antler production and rapid somatic growth. In areas where Na concentration in forage is below dietary require...
- Published
- 2002
- Full Text
- View/download PDF
26. Sodium Provision and Wild Cottontail Rabbits: Morphological Change in Adrenal Glands
- Author
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Clark D. McCreedy and Harmon P. Weeks
- Subjects
Herbivore ,Ecology ,Adrenal cortex ,Sodium ,Wildlife ,Zoology ,chemistry.chemical_element ,Histology ,Biology ,Fecundity ,medicine.anatomical_structure ,Nutrient ,chemistry ,Seasonal breeder ,medicine ,General Earth and Planetary Sciences ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation ,General Environmental Science - Abstract
A direct relationship exists between sodium (Na) nutrition and morphology of the mammalian adrenal cortex, but little research on the relationship between soil Na levels and herbivore populations exists. Consequently, we assessed the effect of Na on wild cottontail rabbits (Sylvilagus floridanus) inhabiting Na poor soils in southcentral Indiana by characterizing their adrenal mass and histology prior to, and following, provision of supplemental Na. Data and tissues were collected at annual (1985-87) hunter check stations and during the 1987 breeding season at the Atterbury Fish and Wildlife Area
- Published
- 1992
- Full Text
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27. Salt Preferences and Sodium Drive Phenology in Fox Squirrels and Woodchucks
- Author
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Harmon P. Weeks and Charles M. Kirkpatrick
- Subjects
Ecology ,Phenology ,animal diseases ,Sodium ,Potassium ,Dietary intake ,chemistry.chemical_element ,Zoology ,Biology ,Seasonality ,medicine.disease ,biology.organism_classification ,chemistry ,parasitic diseases ,Genetics ,medicine ,Animal Science and Zoology ,Licking ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation ,Animal use ,Sciurus - Abstract
Salt preferences and sodium (Na) drive were examined in southern Indiana populations of fox squirrels ( Sciurus niger ) and woodchucks ( Marmota monax ). Animal use of salt-impregnated wooden pegs demonstrated that Na compounds were highly preferred. Both seasonal variation in peg use and the phenology of animals licking the road surface for residues of winter-applied sodium chloride demonstrated definite seasonal variation in Na drive. Spring peaks for both species and an additional autumn peak in squirrels were clearly associated with sudden increases in dietary intake of potassium (K) in spring and decreases in Na intake in squirrels in autumn. The high K intake is postulated to interfere with otherwise efficient Na-conserving mechanisms.
- Published
- 1978
- Full Text
- View/download PDF
28. Glucocorticoids in Eastern Cottontail Rabbits (Sylvilagus floridanus)
- Author
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Gary D. Hamilton and Harmon P. Weeks
- Subjects
medicine.medical_specialty ,Ecology ,biology ,Zoology ,biology.organism_classification ,chemistry.chemical_compound ,Endocrinology ,Induced stress ,chemistry ,Corticosterone ,Internal medicine ,Genetics ,Eastern cottontail ,medicine ,Animal Science and Zoology ,hormones, hormone substitutes, and hormone antagonists ,Ecology, Evolution, Behavior and Systematics ,Glucocorticoid ,Nature and Landscape Conservation ,medicine.drug - Abstract
Most of the information available on lagomorph adrenocorticosteroid physiology is from work with Oryctolagus cuniculus . This species is known to secrete corticosterone as the primary glucocorticoid but may be able to produce cortisol under induced stress conditions. Because of a paucity of information from other lagomorph genera, the generalization has emerged that all lagomorphs predominantly secrete corticosterone. In this study cortisol was shown to be the principal glucocorticoid in Sylvilagus floridanus in both stressed and unstressed physiological states, as had previously been established for Lepus americanus .
- Published
- 1985
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29. Acute Inversion of the Uterus
- Author
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A. P. Weeks
- Subjects
Fuel Technology ,business.industry ,Energy Engineering and Power Technology ,Medicine ,General Medicine ,Geophysics ,business ,Inversion (discrete mathematics) - Abstract
n/a
- Published
- 1886
- Full Text
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30. Water use by saltcedar and by replacement vegetation in the Pecos River floodplain between Acme and Artesia, New Mexico
- Author
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B. D. Tanner, H. L. Weaver, G. S. Campbell, and E. P. Weeks
- Subjects
geography ,geography.geographical_feature_category ,Floodplain ,medicine ,Environmental science ,Forestry ,medicine.symptom ,Vegetation (pathology) ,Water use - Published
- 1987
- Full Text
- View/download PDF
31. Cortisol and aldosterone comparisons of cottontail rabbits collected by shooting, trapping, and falconry
- Author
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Gary D. Hamilton and Harmon P. Weeks
- Subjects
Male ,medicine.medical_specialty ,Aldosterone ,Ecology ,Hydrocortisone ,Animals, Wild ,Aldosterone levels ,Biology ,biology.organism_classification ,Specimen Handling ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,medicine ,Eastern cottontail ,Animals ,Female ,Rabbits ,Cortisol level ,Ecology, Evolution, Behavior and Systematics - Abstract
Cortisol and aldosterone levels were measured in plasma of eastern cottontail rabbits (Sylvilagus floridanus) collected by three different methods, i.e., shooting, live-trapping and falconry. Cortisol levels ranged from near 0 to 27.5 micrograms/100 ml and aldosterone from near 0 to 220 ng/100 ml. Shot animals had significantly lower cortisol concentrations than those taken by either of the other methods. Trapped cottontails also had significantly lower aldosterone levels.
- Published
- 1985
32. Induction and synthesis of tubulin during the cell cycle and life cycle of Chlamydomonas reinhardi
- Author
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Philip S. Collis and Donald P. Weeks
- Subjects
Cell division ,Somatic cell ,Zygote ,Cellular differentiation ,Cell ,Tubulin ,medicine ,Molecular Biology ,Glycoproteins ,Genetics ,biology ,Chlamydomonas ,Cell Cycle ,Cell Differentiation ,Cell Biology ,Cell cycle ,biology.organism_classification ,Cell biology ,Molecular Weight ,medicine.anatomical_structure ,Flagella ,biology.protein ,Female ,Cytokinesis ,Developmental Biology - Abstract
Two types of tubulin induction are observed in Chlamydomonas reinhardi . One is elicited by flagellar detachment and the other occurs as a normal event of the vegetative cell cycle. In the former case, a strong and extensive induction of tubulin synthesis occurs following deflagellation of cells in all phases of the life cycle [vegetative, gametic, and (early) zygotic]. Synthesis is initiated in all three cell types within 15 min after deflagellation. In gametic and zygotic cells, tubulin synthesis so induced accounts for 15 to 20% of the total protein synthesis during the 1-hr peak period of tubulin production. The ability to support both tubulin synthesis and flagellar regeneration is lost in zygotes at 1.5 hr after the initiation of zygotic development. This alteration represents one of several dramatic shifts in the programming of protein synthesis that occur during the first 4 hr of zygotic differentiation in C. reinhardi . The second (i.e., cell cycle-dependent) type of induction is observed in synchronously growing vegetative cells at ∼1.5–2 hr prior to cytokinesis. Tubulin synthesis, in this case, persists at relatively high levels (∼5% of the total protein synthesis) for the next 9 hr, i.e., through the entire period of cell division to a time just before the liberation of fully flagellated daughter cells at hr 20 of the cell cycle. Changes in the programming of protein synthesis, and of tubulin synthesis in particular, are discussed in relation to specific physiological and cytological transitions that occur during the growth and differentiation of C. reinhardi .
- Published
- 1979
33. Piroxicam monohydrate: a zwitterionic form, C15H13N3O4S.H2O
- Author
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P. Weeks, J. Bordner, J. A. Richards, and E. B. Whipple
- Subjects
Chemistry ,Polymer chemistry ,medicine ,General Medicine ,Crystal structure ,Piroxicam ,General Biochemistry, Genetics and Molecular Biology ,medicine.drug - Published
- 1984
- Full Text
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34. Campaign Promotes Discount Drug Program.
- Author
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Weeks, Katie
- Subjects
MEDICINE ,DRUGS ,PHARMACEUTICAL industry ,ACTIONS & defenses (Law) ,MEDICAL marijuana ,ASSOCIATIONS, institutions, etc. ,VIDEOCONFERENCING - Abstract
The article reports developments related to medicine in California. Pharmaceutical companies are promoting their discount drug program by recruiting talk show host Montel Williams. The San Diego County has filed a lawsuit in federal court to challenge the state law that allows the use of medical marijuana. The Council of Community Clinics used a grant from the California Telemedicine and eHealth Center to offer videoconference doctor visits to residents in rural areas.
- Published
- 2006
35. OBITUARY.
- Author
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Browne Jr., Edward Weeks
- Subjects
MEDICINE - Abstract
An obituary for Edward Weeks Browne, member of Alpha Omega Alpha Honor Medical Society is presented.
- Published
- 2012
36. Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing.
- Author
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Eric A Noel, Donald P Weeks, and James L Van Etten
- Subjects
Medicine ,Science - Abstract
Genetic and molecular modifications of the large dsDNA chloroviruses, with genomes of 290 to 370 kb, would expedite studies to elucidate the functions of both identified and unidentified virus-encoded proteins. These plaque-forming viruses replicate in certain unicellular, eukaryotic chlorella-like green algae. However, to date, only a few of these algal species and virtually none of their viruses have been genetically manipulated due to lack of practical methods for genetic transformation and genome editing. Attempts at using Agrobacterium-mediated transfection of chlorovirus host Chlorella variabilis NC64A with a specially-designed binary vector resulted in successful transgenic cell selection based on expression of a hygromycin-resistance gene, initial expression of a green fluorescence gene and demonstration of integration of Agrobacterium T-DNA. However, expression of the integrated genes was soon lost. To develop gene editing tools for modifying specific chlorovirus CA-4B genes using preassembled Cas9 protein-sgRNA ribonucleoproteins (RNPs), we tested multiple methods for delivery of Cas9/sgRNA RNP complexes into infected cells including cell wall-degrading enzymes, electroporation, silicon carbide (SiC) whiskers, and cell-penetrating peptides (CPPs). In one experiment two independent virus mutants were isolated from macerozyme-treated NC64A cells incubated with Cas9/sgRNA RNPs targeting virus CA-4B-encoded gene 034r, which encodes a glycosyltransferase. Analysis of DNA sequences from the two mutant viruses showed highly targeted nucleotide sequence modifications in the 034r gene of each virus that were fully consistent with Cas9/RNP-directed gene editing. However, in ten subsequent experiments, we were unable to duplicate these results and therefore unable to achieve a reliable system to genetically edit chloroviruses. Nonetheless, these observations provide strong initial suggestions that Cas9/RNPs may function to promote editing of the chlorovirus genome, and that further experimentation is warranted and worthwhile.
- Published
- 2021
- Full Text
- View/download PDF
37. Efficient CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana and inheritance of modified genes in the T2 and T3 generations.
- Author
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WenZhi Jiang, Bing Yang, and Donald P Weeks
- Subjects
Medicine ,Science - Abstract
The newly developed CRISPR/Cas9 system for targeted gene knockout or editing has recently been shown to function in plants in both transient expression systems as well as in primary T1 transgenic plants. However, stable transmission of genes modified by the Cas9/single guide RNA (sgRNA) system to the T2 generation and beyond has not been demonstrated. Here we provide extensive data demonstrating the efficiency of Cas9/sgRNA in causing modification of a chromosomally integrated target reporter gene during early development of transgenic Arabidopsis plants and inheritance of the modified gene in T2 and T3 progeny. Efficient conversion of a nonfunctional, out-of-frame GFP gene to a functional GFP gene was confirmed in T1 plants by the observation of green fluorescent signals in leaf tissues as well as the presence of mutagenized DNA sequences at the sgRNA target site within the GFP gene. All GFP-positive T1 transgenic plants and nearly all GFP-negative plants examined contained mutagenized GFP genes. Analyses of 42 individual T2 generation plants derived from 6 different T1 progenitor plants showed that 50% of T2 plants inherited a single T-DNA insert. The efficiency of the Cas9/sgRNA system and stable inheritance of edited genes point to the promise of this system for facile editing of plant genes.
- Published
- 2014
- Full Text
- View/download PDF
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