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2. Trophectoderm biopsy protocols can affect clinical outcomes: time to focus on the blastocyst biopsy technique

Author

P. Rubino, Lisa Guan, J.M. Norian, J. Nelson, Caroline Moon, B. Kolb, Lucia Tapia, J. Wilcox, Kohar Mazmanian, A. Thiel, Rafael Ruiz de Assin Alonso, Lindsay Dearden, and T. Tan

Subjects
0301 basic medicine, Pregnancy Rate, Biopsy, Monozygotic twin, Aneuploidy, Fertilization in Vitro, Risk Assessment, Andrology, 03 medical and health sciences, 0302 clinical medicine, Predictive Value of Tests, Pregnancy, Risk Factors, Medicine, Humans, Blastocyst, Genetic Testing, Zona pellucida, Survival rate, Preimplantation Diagnosis, Retrospective Studies, 030219 obstetrics & reproductive medicine, medicine.diagnostic_test, business.industry, Blastocyst Transfer, Obstetrics and Gynecology, medicine.disease, Embryo Transfer, 030104 developmental biology, medicine.anatomical_structure, Treatment Outcome, Reproductive Medicine, embryonic structures, Female, business, Live birth, Live Birth
Abstract

Objective To compare two different blastocyst biopsy protocols. Design Retrospective single-center cohort study. Settings Private in vitro fertilization center. Patient(s) The study included 1,670 frozen-thawed embryo transfers (FETs) with preimplantation genetic testing for aneuploidy (PGT-A). Intervention None. Main Outcome Measure(s) Survival rate (SR) after thawing, clinical pregnancy rate (CPR), ongoing implantation rate (IR), and live birth rate (LBR). Result(s) Eight hundred thirty-five FETs with PGT-A cycles including only embryos biopsied in the sequential blastocyst hatching and biopsy protocol paired with the ablation of one-fourth of the zona pellucida (ZP) were matched with 835 FETs with PGT-A cycles including only embryos biopsied in the day 3 prehatching protocol by female age (±1 year), number of embryos transferred, use of gestational carrier or egg donor, and day of blastocyst transfer. Only FETs with euploid blastocysts graded no lower than 4BB were included, and cycles with fewer than five oocytes were excluded. SR after thawing, CPR, ongoing IR, and LBR were significantly higher in the FET cycles with the embryos biopsied in the sequential hatching and biopsy protocol. Four cases of monozygotic twin pregnancies were reported with the day 3 prehatching protocol and none with the sequential hatching and biopsy protocol. Conclusion(s) Our results show, for the first time, that using different blastocyst biopsy protocols can affect clinical outcomes. Because the study was retrospective, our findings should be validated in a prospective trial.

Published
2019

4. The blastocyst re-expansion status after thawing do not seem to affect the clinical outcomes in freeze all preimplantation genetic testing (PGT) cycles

Author

A. Thiel, P. Rubino, L. Tapia, J.M. Norian, K. Mazmanian, J. Wilcox, J. Nelson, L. Dearden, B. Kolb, L. Guan, T. Tan, and R. Ruiz de Assin Alonso

Subjects
Andrology, Freeze all, medicine.anatomical_structure, Reproductive Medicine, Re expansion, medicine.diagnostic_test, medicine, Obstetrics and Gynecology, Blastocyst, Biology, Affect (psychology), Genetic testing
Published
2019
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5. Revisiting embryo assisted hatching approaches: a systematic review of the current protocols

Author

Paola Viganò, P. Rubino, Ahmad Abu Maizar, Elisa Giacomini, Luca Jovine, and Alessandra Alteri

Subjects
0301 basic medicine, animal structures, Reproductive Techniques, Assisted, medicine.medical_treatment, Context (language use), Fertilization in Vitro, Review, Biology, Andrology, 03 medical and health sciences, Micromanipulation, 0302 clinical medicine, Pregnancy, Genetics, medicine, Animals, Humans, Embryo Implantation, Zona pellucida, Genetics (clinical), Zona Pellucida, Cryopreservation, 030219 obstetrics & reproductive medicine, Assisted reproductive technology, Hatching, Lasers, Obstetrics and Gynecology, Embryo culture, Embryo, General Medicine, Embryo Transfer, Assisted hatching, 030104 developmental biology, medicine.anatomical_structure, Reproductive Medicine, embryonic structures, Female, Biological plausibility, Developmental Biology, Systematic Reviews as Topic
Abstract

Zona pellucida (ZP) manipulation, termed "assisted hatching" (AH), has been introduced in order to favor embryo hatching and ultimately improve assisted reproductive technology success but with poor proofs of safety and biological plausibility. We herein provide a systematic review of clinical outcomes following the application of different methods of ZP manipulation on fresh or frozen/thawed embryos at different developmental stages in different groups of patients. Out of the 69 papers that compared the clinical outcomes deriving from hatched versus non-hatched embryos, only 11 considered blastocysts while the rest referred to cleavage stage embryos. The ZP thinning of fresh embryos either by chemical or laser approach was shown to provide very limited benefit in terms of clinical outcomes. Better results were observed with procedures implying a higher degree of zona manipulation, including zona removal. Studies comparing the mechanical or chemical procedures to those laser-mediated consistently reported a superiority of the latter ones over the former. Literature is consistent for a benefit of ZP breaching in thawed blastocysts. This review provides the current knowledge on the AH procedure in order to improve its efficacy in the appropriate context. Embryologists might benefit from the approaches presented herein in order to improve Assisted Reproduction Technologies (ART) outcomes.

Published
2017

6. A direct healthcare cost analysis of the cryopreserved versus fresh transfer policy at the blastocyst stage

Author

P. Rubino, Paola Viganò, Massimo Candiani, Luca Pagliardini, Diana Delprato, Valeria Stella Vanni, Enrico Papaleo, Papaleo, Enrico, Pagliardini, Luca, Vanni, Valeria Stella, Delprato, Diana, Rubino, Patrizia, Candiani, Massimo, and Viganò, Paola

Subjects
0301 basic medicine, Oocyte, Pregnancy Rate, Cost-Benefit Analysis, Cryopreservation, 0302 clinical medicine, Human fertilization, Pregnancy, Retrospective Studie, Cost analysi, Birth Rate, Freeze-all, 030219 obstetrics & reproductive medicine, Obstetrics and Gynecology, Health Care Costs, Embryo transfer, medicine.anatomical_structure, Italy, IVF, Female, Live birth, Case-Control Studie, Human, Infertility, Adult, medicine.medical_specialty, Fertilization in Vitro, 03 medical and health sciences, medicine, Humans, Blastocyst, Cost-Benefit Analysi, Retrospective Studies, Gynecology, business.industry, medicine.disease, Embryo Transfer, Vitrification, Health Care Cost, 030104 developmental biology, Reproductive Medicine, Case-Control Studies, Oocytes, business, Body mass index, Developmental Biology
Abstract

A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030). A mean per live birth cost-saving of €2178 (95% CI −1810 to 6165) resulted in a freeze-all strategy owing to fewer embryo transfer procedures (1.29 ± 0.5 versus 1.41 ± 0.7); differences were not significant. Sensitivity analysis revealed that the freeze-all strategy remained cost-effective until the live birth rate is either higher or only slightly lower (≥–0.59%) in the freeze-all group compared with fresh cycles. A freeze-all policy does not increase costs compared with fresh transfer, owing to negligible additional expenses, i.e. vitrification, endometrial priming and monitoring, against fewer embryo transfer procedures required to achieve pregnancy.

Published
2017

7. Trophectoderm (TE) biopsy protocols can affect clinical results. time to focus on the biopsy technique

Author

L. Dearden, Luca Pagliardini, J.M. Norian, B. Kolb, A. Thiel, L. Hernandez, R. Ruiz de Assin Alonso, L. Guan, J. Nelson, J. Wilcox, P. Rubino, K. Mazmanian, and T. Tan

Subjects
medicine.medical_specialty, Focus (computing), Reproductive Medicine, medicine.diagnostic_test, business.industry, Biopsy, medicine, Obstetrics and Gynecology, Medical physics, business, Affect (psychology)
Published
2018
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8. The impact of cryopreservation on biopsied embryos without the bias of the uterine receptivity. retrospective analysis of 493 PGS cycles involving egg donors (ED)/gestational carriers (GC)

Author

J. Wilcox, J. Nelson, P. Sinogaya, P. Rubino, L. Guan, J.M. Norian, L. Tapia, R. Ruiz de Assin, B. Kolb, and T. Tan

Subjects
Andrology, Gynecology, Egg donation, Gestational carrier, medicine.medical_specialty, Reproductive Medicine, Retrospective analysis, medicine, Obstetrics and Gynecology, Embryo, Uterine receptivity, Biology, Cryopreservation
Published
2017
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9. POSTER VIEWING SESSION - ANDROLOGY

Author

E. C. Dul, C. M. A. van Ravenswaaij-Arts, H. Groen, J. van Echten-Arends, J. A. Land, Y. Tyulenev, V. Naumenko, L. Kurilo, L. Shileiko, A. Segal, R. Klimova, A. Kushch, J. Ribas-Maynou, A. Garcia-Peiro, C. Abad, M. J. Amengual, J. Benet, J. Navarro, A. Colasante, A. M. Lobascio, F. Scarselli, M. G. Minasi, E. Alviggi, P. Rubino, V. Casciani, R. Pena, M. T. Varricchio, K. Litwicka, S. Ferrero, D. Zavaglia, G. Franco, Z. P. Nagy, E. Greco, L. Romany, M. Meseguer, S. Garcia-Herrero, A. Pellicer, N. Garrido, A. Dam, A. Pijnenburg, J. C. Hendriks, J. R. Westphal, L. Ramos, J. A. M. Kremer, F. Eertmans, V. Bogaert, B. Puype, W. Geisler, C. Clusmann, I. Klopsch, T. Strowitzki, W. Eggert-Kruse, R. Maettner, E. Isachenko, V. Isachenko, E. Strehler, K. Sterzik, G. Band, I. Madgar, H. Brietbart, Z. Naor, J. S. Cunha-Filho, C. A. Souza, V. G. Krebs, K. D. Santos, W. J. Koff, A. Stein, I. Hammoud, M. Albert, M. Bergere, M. Bailly, F. Boitrelle, F. Vialard, R. Wainer, V. Izard, J. Selva, P. Cohen - Bacrie, S. Belloc, J. de mouzon, M. Cohen-Bacrie, S. Alvarez, A. M. Junca, M. Dumont, S. Douard, N. Prisant, K. Tomita, S. Hashimoto, Y. Akamatsu, M. Satoh, R. Mori, T. Inoue, Y. Ohnishi, K. Ito, Y. Nakaoka, Y. Morimoto, V. J. H. Smith, K. K. Ahuja, F. Atig, M. Raffa, M. T. Sfar, A. Saad, M. Ajina, D. P. A. F. Braga, G. Halpern, R. C. S. Figueira, A. S. Setti, A. Iaconelli Jr., E. Borges Jr., G. S. Medeiros, E. B. Pasqualotto, F. F. Pasqualotto, M. Nadalini, N. Tarozzi, M. Di Santo, A. Borini, C. Lopez-Fernandez, F. Arroyo, P. Caballero, R. Nunez-Calonge, J. L. Fernandez, J. Gosalvez, A. Gosalbez, S. Cortes, K. Zikopoulos, L. Lazaros, G. Vartholomatos, A. Kaponis, G. Makrydimas, N. Plachouras, N. Sofikitis, S. Kalantaridou, E. Hatzi, I. Georgiou, J. de Mouzon, E. Amar, P. Cohen-Bacrie, M. L. Vuillaume, F. Brugnon, C. Artonne, L. Janny, H. Pons-Rejraji, J. Fedder, L. Bosco, G. Ruvolo, A. M. Bruccoleri, M. Manno, M. C. Roccheri, E. Cittadini, I. Bochev, P. Gavrilov, S. Kyurkchiev, A. Shterev, G. Carlomagno, M. Colone, R. A. Condorelli, A. Stringaro, A. E. Calogero, J. Zakova, M. Kralikova, I. Crha, P. Ventruba, J. Melounova, M. Matejovicova, M. Vodova, E. Lousova, M. Sanchez Toledo, C. Alvarez LLeo, C. Garcia Garrido, M. Resta Serra, L. L. Belmonte Andujar, G. Gonzalez de Merlo, M. Pohanka, M. Huser, I. Amiri, J. Karimi, M. T. Goodarzi, H. Tavilani, A. Filannino, M. C. Magli, E. Boudjema, A. Crippa, A. P. Ferraretti, L. Gianaroli, F. Robles, H. Huang, D. J. Yao, H. J. Huang, J. R. Li, S. K. Fan, M. L. Wang, S. Yung-Kuei, S. Amer, A. Mahran, J. Darne, R. Shaw, E. Borghi, C. Cetera, U. Shukla, D. Ogutu, B. Deval, M. Jansa, M. Savvas, N. Narvekar, P. Houska, A. L. Dackland, L. Bjorndahl, U. Kvist, L. Muzii, B. Barboni, L. Samanta, S. Kar, S. A. Yakovenko, M. N. Troshina, B. K. Rutman, S. A. Dyakonov, E. Holmes, C. Feijo, S. Verza Junior, S. C. Esteves, C. L. Berta, A. M. Caille, S. A. Ghersevich, C. Zumoffen, M. J. Munuce, M. San Celestino, D. Agudo, M. Alonso, P. Sanjurjo, D. Becerra, F. Bronet, J. A. Garcia-Velasco, A. Pacheco, R. Lafuente, G. Lopez, M. A. Checa, R. Carreras, M. Brassesco, M. Oneta, V. Savasi, B. Parrilla, D. Guarneri, A. Laureti, F. Pagano, I. Cetin, E. Ekwurtzel, G. Morgante, P. Piomboni, A. Stendardi, F. Serafini, V. De Leo, R. Focarelli, M. Benkhalifa, J. De Mouzon, F. Entezami, A. Junca, J. J. De Mouzon, A. Mangiarini, E. Capitanio, A. Paffoni, L. Restelli, C. Guarneri, C. Scarduelli, G. Ragni, K. Harrison, J. Irving, N. Martin, D. Sherrin, A. Yazdani, C. Almeida, S. Correia, E. Rocha, A. Alves, M. Cunha, L. Ferraz, S. Silva, M. Sousa, A. Barros, A. Perdrix, A. Travers, J. P. Milazzo, F. Clatot, N. Mousset-Simeon, B. Mace, N. Rives, H. S. Clarke, A. Callow, D. Saxton, A. A. Pacey, O. Sapir, G. Oron, A. Ben-Haroush, R. Garor, D. Feldberg, H. Pinkas, A. Wertheimer, B. Fisch, E. Palacios, M. C. Gonzalvo, A. Clavero, J. P. Ramirez, A. Rosales, J. Mozas, J. A. Castilla, J. Mugica, O. Ramon, A. Valdivia, A. Exposito, L. Casis, R. Matorras, R. Bongers, F. Gottardo, M. Zitzmann, S. Kliesch, T. Cordes, A. Kamischke, A. Schultze-Mosgau, N. Buendgen, K. Diedrich, G. Griesinger, L. Crisol, F. Aspichueta, M. L. Hernandez, J. I. Ruiz-Sanz, R. Mendoza, A. A. Sanchez-Tusie, A. Bermudez, P. Lopez, G. C. Churchill, C. L. Trevino, I. Maldonado, and J. Dabbah

Subjects
medicine.medical_specialty, Reproductive Medicine, Rehabilitation, medicine, Obstetrics and Gynecology, Medical physics, Session (computer science), Psychology
Published
2011
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10. POSTER VIEWING SESSION - EMBRYOLOGY

Author

S. Fourati Ben Mustapha, M. Khrouf, K. Kacem Ben Rejeb, H. Elloumi Chaabene, G. Merdassi, D. Wahbi, M. Ben Meftah, F. Zhioua, A. Zhioua, A. Azzarello, T. Host, A. L. Mikkelsen, C. P. Theofanakis, V. Dinopoulou, D. Mavrogianni, G. A. Partsinevelos, P. Drakakis, K. Stefanidis, A. Bletsa, D. Loutradis, L. Rienzi, A. Cobo, A. Paffoni, C. Scarduelli, A. Capalbo, N. Garrido, J. Remohi, G. Ragni, F. M. Ubaldi, R. Herrer, M. Quera, E. GIL, J. Serna, M. L. Grondahl, J. Bogstad, I. E. Agerholm, J. G. Lemmen, U. Bentin-Ley, P. Lundstrom, U. S. Kesmodel, M. Raaschou-Jensen, S. Ladelund, L. Guzman, C. Ortega, F. K. Albuz, R. B. Gilchrist, P. Devroey, J. Smitz, M. De Vos, M. Bielanska, M. C. Leveille, E. Borghi, M. C. Magli, M. J. Figueroa, G. Mascaretti, A. P. Ferraretti, L. Gianaroli, E. Szlit, F. Leocata Nieto, G. Maggiotto, G. Arenas, N. Tarducci Bonfiglio, A. Ahumada, R. Asch, R. Sciorio, N. Dayoub, J. Thong, S. Pickering, J. Ten, M. A. Carracedo, J. Guerrero, A. Rodriguez-Arnedo, J. Llacer, R. Bernabeu, C. Tatone, T. Heizenrieder, G. Di Emidio, P. Treffon, T. Seidel, U. Eichenlaub-Ritter, S. S. Cortezzi, E. C. Cabral, C. R. Ferreira, M. G. Trevisan, R. C. S. Figueira, D. P. A. F. Braga, M. N. Eberlin, A. Iaconelli Jr., E. Borges Jr., A. Zabala, T. Pessino, L. Blanco, G. Rey Valzacchi, F. Leocata, F. Vanden Meerschaut, B. Heindryckx, C. Qian, D. Deforce, L. Leybaert, P. De Sutter, M. De las Heras, J. L. De Pablo, B. Navarro, J. A. Agirregoikoa, G. Barrenetxea, M. Cruz, I. Perez-Cano, B. Gadea, J. Herrero, M. Martinez, M. Roldan, M. Munoz, A. Pellicer, M. Meseguer, N. Galindo, F. Scarselli, E. Alviggi, A. Colasante, M. G. Minasi, P. Rubino, M. Lobascio, S. Ferrero, K. Litwicka, M. T. Varricchio, P. Giannini, P. Piscitelli, G. Franco, D. Zavaglia, Z. P. Nagy, E. Greco, F. Urner, D. Wirthner, F. Murisier, P. Mock, M. Germond, B. Amorocho Llanos, G. Calderon, D. Lopez, L. Fernandez, M. Nicolas, J. Landeras, S. L. Finn-Sell, R. Leandri, T. P. Fleming, N. S. Macklon, Y. C. Cheong, J. J. Eckert, J. H. Lee, Y. J. Jung, H. K. Hwang, A. Kang, S. J. An, J. Y. Jung, H. C. Kwon, S. J. Lee, S. Palini, L. Zolla, S. De Stefani, V. Scala, A. D'Alessandro, V. Polli, P. Rocchi, A. Tiezzi, E. Pelosi, L. Dusi, C. Bulletti, R. Fadini, M. Lain, M. Mignini Renzini, F. Brambillasca, G. Coticchio, M. Merola, M. C. Guglielmo, M. Dal Canto, R. Figueira, A. S. Setti, K. C. Worrilow, C. D. Uzochukwu, S. Eid, S. Le Gac, T. C. Esteves, F. van Rossem, A. van den Berg, M. Boiani, E. Kasapi, Y. Panagiotidis, M. Goudakou, A. Papatheodorou, T. Pasadaki, N. Prapas, Y. Prapas, P. Vanderzwalmen, S. Norasing, P. Atchajaroensatit, W. Tawiwong, O. Thepmanee, S. Saenlao, J. Aojanepong, P. Hunsajarupan, K. Sajjachareonpong, P. Punyatanasakchai, S. Maneepalviratn, U. Jetsawangsri, A. Tejera, I. Rubio, J. L. Romero, V. Nordhoff, S. Schlatt, A. N. Schuring, L. Kiesel, S. Kliesch, R. Azambuja, L. Okada, V. Lazzari, L. Dorfman, J. Michelon, M. Badalotti, F. Badalotti, A. Petracco, C. Schwarzer, K. Versieren, I. De Croo, S. Lierman, W. De Vos, E. Van den Abbeel, J. Gerris, I. Milacic, D. Borogovac, M. Veljkovic, B. Arsic, D. Jovic Bojovic, D. Lekic, D. Pavlovic, E. Garalejic, D. F. Albertini, E. De Ponti, F. Sanges, R. Talevi, L. Papini, V. Mollo, L. F. Rienzi, R. Gualtieri, C. Orteg, J. Choi, H. Lee, S. Ku, S. Kim, Y. Choi, J. Kim, S. Moon, E. Demilly, S. Assou, S. Moussaddykine, H. Dechaud, S. Hamamah, T. Takisawa, M. Doshida, H. Hattori, Y. Nakamura, T. Kyoya, Y. Shibuya, Y. Nakajo, A. Tasaka, M. Toya, K. Kyono, S. Novo, O. Penon, R. Gomez, L. Barrios, M. Duch, J. Santalo, J. Esteve, C. Nogues, J. A. Plaza, L. Perez-Garcia, E. Ibanez, S. Chavez, K. Loewke, B. Behr, R. Reijo Pera, S. Huang, H. Wang, Y. Soong, C. Chang, T. Okimura, M. Kuwayama, C. Mori, M. Morita, K. Uchiyama, F. Aono, K. Kato, Y. Takehara, O. Kato, M. Minasi, V. Casciani, L. Arizzi, C. Mencacci, C. Piscitelli, F. Cucinelli, A. Tocci, E. Wydooghe, L. Vandaele, J. Dewulf, A. Van Soom, J. H. Moon, W. Y. Son, A. Mahfoudh, S. Henderson, S. G. Jin, E. Shalom-Paz, M. Dahan, H. Holzer, K. Mahmoud, C. Triki-Hmam, K. Terras, T. Hfaiedh, M. H. Ben Aribia, H. Otsubo, A. Egashira, K. Tanaka, T. Matsuguma, M. Murakami, K. Murakami, M. Otsuka, N. Yoshioka, Y. Araki, T. Kuramoto, J. G. Smit, M. D. Sterrenburg, M. J. C. Eijkemans, H. G. Al-Inany, M. A. F. M. Youssef, F. J. M. Broekmans, K. Willoughby, L. DiPaolo, L. Deys, A. Lagunov, S. Amin, M. Faghih, E. Hughes, M. Karnis, F. Ashkar, W. A. King, M. S. Neal, I. Antonova, L. Veleva, L. Petkova, A. Shterev, C. Nogales, E. Martinez, M. Ariza, D. Cernuda, M. Gaytan, A. Linan, A. Guillen, F. Bronet, V. Cottin, D. Fabian, F. Allemann, A. Koller, J. C. Spira, D. Agudo, M. Martinez-Burgos, A. Arnanz, N. Basile, A. Rodriguez, Y. S. Cho, M. Filioli Uranio, B. Ambruosi, M. S. Paternoster, P. Totaro, A. M. Sardanelli, M. E. Dell'Aquila, U. Zollner, T. Hofmann, K. P. Zollner, B. Kovacic, P. Roglic, V. Vlaisavljevic, M. Sole, M. Boada, B. Coroleu, A. Veiga, G. Martiny, M. Molinari, A. Revelli, N. M. Chimote, M. Chimote, B. Mehta, N. N. Chimote, N. Sheikh, N. Nath, A. Mukherjee, K. Rakic, M. Reljic, H. J. Ingerslev, K. Kirkegaard, J. Hindkjaer, I. Agerholm, H. Kitasaka, N. Fukunaga, R. Nagai, T. Yoshimura, F. Tamura, K. Kitamura, N. Hasegawa, K. Nakayama, M. Katou, F. Itoi, E. Asano, N. Deguchi, K. Ooyama, Y. Hashiba, Y. Asada, M. Michaeli, N. Rotfarb, E. Karchovsky, O. Ruzov, R. Atamny, K. Slush, O. Fainaru, A. Ellenbogen, S. Chekuri, T. Chaisrisawatsuk, P. Chen, M. Pangestu, S. Jansen, S. Catt, E. Molinari, C. Racca, C. Ryu, S. Kang, J. Lee, D. Chung, S. Roh, H. Chi, Y. Yokota, M. Yokota, H. Yokota, S. Sato, M. Nakagawa, M. Komatsubara, M. Makita, K. Oyama, K. Naruse, S. Kilani, M. G. Chapman, M. Kwik, M. Chapman, S. Guven, E. Odaci, O. Yildirim, C. Kart, M. A. Unsal, E. Yulug, E. Isachenko, R. Maettner, E. Strehler, V. Isachenko, K. Hancke, R. Kreienberg, K. Sterzik, X. Y. Zheng, L. N. Wang, P. Liu, J. Qiao, F. Inoue, M. Dashtizad, H. Wahid, Y. Rosnina, M. Daliri, H. Hajarian, M. Akbarpour, O. Abbas Mazni, K. Knez, T. Tomaevic, E. Vrtacnik Bokal, B. Zorn, I. Virant Klun, M. Koster, J. Liebenthron, A. Nicolov, K. van der Ven, H. van der Ven, M. Montag, M. Fayazi, M. Salehnia, M. Beigi Boroujeni, B. Khansarinejad, K. Deignan, G. Emerson, E. Mocanu, J. J. Wang, M. Andonov, E. Linara, K. K. Ahuja, S. Nachef, F. F. Pasqualotto, E. Pasqualotto, C. C. Chang, D. P. Bernal, T. A. Elliott, D. B. Shapiro, A. A. Toledo, K. Economou, S. Davies, M. Argyrou, S. Doriza, P. Sisi, M. Moschopoulou, A. Karagianni, C. Mendorou, N. Polidoropoulos, C. Papanicopoulos, P. Stefanis, C. Karamalegos, H. Cazlaris, M. Koutsilieris, M. Mastrominas, S. Gotts, A. Doshi, J. Harper, P. Serhal, A. Borini, O. Guzeloglu-Kayisli, V. Bianchi, E. Seli, M. Lappi, M. A. Bonu, S. Mizuta, H. Hashimoto, Y. Kuroda, Y. Matsumoto, Y. Mizusawa, S. Ogata, S. Yamada, S. Kokeguchi, Y. Noda, M. Shiotani, M. Stojkovic, M. Ilic, N. Markovic, P. Stojkovic, G. Feng, B. Zhang, H. Zhou, L. Zhou, X. Gan, X. Qin, J. Shu, F. Wu, I. Molina Botella, E. Lazaro Ibanez, A. Debon Aucejo, J. Pertusa, P. J. Fernandez Colom, C. Li, Y. Zhang, Y. Cui, H. Zhao, J. Liu, J. B. A. Oliveira, C. G. Petersen, A. L. Mauri, F. C. Massaro, L. F. I. Silva, J. Ricci, M. Cavagna, A. Pontes, L. D. Vagnini, R. L. R. Baruffi, J. G. Franco Jr., V. Felipe, M. Vilela, M. Tiveron, C. Lombardi, M. I. Viglierchio, G. Marconi, V. Rawe, P. L. Wale, D. K. Gardner, K. Nakagawa, R. Sugiyama, Y. Nishi, Y. Kuribayashi, H. Jyuen, E. Yamashiro, A. Shirai, M. Inoue, O. Hovatta, V. Tohonen, J. Inzunza, L. Parmegiani, G. E. Cognigni, S. Bernardi, W. Ciampaglia, F. E. Infante, C. Tabarelli de Fatis, P. Pocognoli, A. Arnone, A. M. Maccarini, E. Troilo, M. Filicori, P. Radwan, I. Polac, M. Borowiecka, M. Bijak, and M. Radwan

Subjects
medicine.medical_specialty, Reproductive Medicine, Embryology, Rehabilitation, medicine, Obstetrics and Gynecology, Medical physics, Session (computer science), Psychology
Published
2011
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11. Posters * Embryology (Embryo Selection)

Author

K. Versieren, B. Heindryckx, C. Qian, J. Gerris, P. De Sutter, A. Exposito Navarro, A. Ametzazurra, D. Nagore, L. Crisol, F. Aspichueta, R. Mendoza, R. Matorras, M. M. Garcia, J. K. Valley, P. S. Swinton, W. J. Boscardin, T. F. Lue, null P. Rinaudo, M. C. Wu, O. Bern, D. Strassburger, D. Komarovsky, E. Kasterstein, A. Komsky, B. Maslansky, A. Raziel, S. Friedler, Y. Gidoni, R. Ron-El, J. Tang, C. Fang, M. F. Zhang, T. Li, G. L. Zhuang, D. S. Suh, J. K. Joo, J. R. Choi, S. C. Kim, M. S. JO, K. H. Kim, K. S. Lee, M. G. Katz-Jaffe, J. Stevens, S. McCormick, R. Smith, W. B. Schoolcraft, I. Ben-Ami, J. Koch, M. Costello, S. Kilani, A. Namm, A. Arend, M. Aunapuu, Y. M. Choi, J. D. Cho, C. Sipe, E. J. Pelts, J. M. Matthews, S. R. Sanchez, R. L. B. Brohammer, Y. Wagner, J. Liebermann, M. Uhler, A. Beltsos, M. J. Chen, H. F. Guu, Y. F. Chen, Y. J. Yih, J. Y. P. Ho, T. Y. Lin, E. S. C. Ho, F. B. Lopes, R. C. S. Figueira, D. P. A. F. Braga, R. C. Ferreira, T. Aoki, A. Iaconelli, E. Borges, H. Van de Velde, G. Cauffman, A. Verloes, C. De Paepe, J. Sterckx, H. Van Ranst, P. Devroey, H. Tournaye, I. Liebaers, M. A. Santos, G. Teklenburg, N. S. Macklon, D. Van Opstal, G. H. Schuring-Blom, P. J. Krijtenburg, J. de Vreeden-Elbertse, B. C. Fauser, E. B. Baart, S. Cawood, A. Doshi, S. Gotts, P. Serhal, T. Milachich, L. Petkova, D. Barov, A. Shterev, T. C. Esteves, S. T. Balbach, M. J. Arauzo-Bravo, M. J. Pfeiffer, M. Boiani, S. Le Gac, F. van Rossem, T. Esteves, M. Bioani, A. van den Berg, C. Valeri, S. Pappalardo, M. De Felici, C. Manna, H. Ryu, C. Y. Park, S. H. Min, S. K. Choi, C. Park, S. H. Lee, K. R. Kim, H. Jeong, H. J. Chi, C. Wittemer, C. Celebi, S. Viville, F. Luceno Maestre, J. A. Castilla Alcala, J. L. Gomez-Palomares, Y. Cabello, J. Hernandez, J. Marqueta, J. Herrero, E. Vidal, S. Fernandez-Shaw, B. Coroleu, C. McRae, E. Baskind, V. Sharma, J. Fisher, P. Boldi Cotti, C. Colasante, L. Perego, L. De Lauretis, M. Montag, M. Koster, A. Nikolov, H. van der Ven, S. G. Lee, Y. C. Lee, S. M. Kang, Y. J. Kang, Y. K. Shin, J. H. Jung, J. H. Lim, A. Dorfmann, K. Carroll, M. Sisson, M. Geltinger, S. Yap, M. Iwaszko, T. Hara, K. Naruse, K. Matsuura, T. Kodama, K. Sato, Y. Tateaki, J. Tanaka, M. G. Minasi, F. Scarselli, P. Rubino, V. Casciani, A. Colasante, M. Lobascio, E. Alviggi, S. Ferrero, K. Litwicka, E. Iammarrone, F. Cucinelli, P. G. Giannini, A. Tocci, Z. P. Nagy, E. Greco, A. Borini, N. Tarozzi, D. Fiorentin, M. A. Bonu, M. Nadalini, J. Johnson, L. De Santis, V. Bianchi, A. M. Lobascio, L. Arizzi, C. Piscitelli, N. Mesut, H. N. Ciray, A. Mesut, T. Aksoy, M. Bahceci, Y. M. Lee, H. W. Chen, P. Wu, C. R. Tzeng, I. Antonova, M. Yunakova, P. Chaveeva, null A. Shterev, D. Hlinka, M. Dudas, J. Rutarova, J. Rezacova, S. Lazarovska, Y. Aoi, H. Takahashi, H. Saitou, C. Takiue, N. Kawakami, M. Tone, R. Hirata, S. Terada, N. Yoshioka, T. Habara, N. Hayashi, J. Montagut, F. Bonald, N. Guillen, V. Guitard, E. Balu-Genvrin, E. Crae, D. Nogueira, J. Silva, M. Cunha, P. Viana, J. M. Teixeira da Silva, C. Oliveira, A. Goncalves, N. Barros, M. Sousa, A. Barros, C. van de Werken, H. Jahr, J. S. E. Laven, P. Gamiz Izquierdo, J. M. De los Santos, A. Tejera, A. Pellicer, J. L. Romero, A. Galan, C. Albert, M. J. D. l. Santos, T. Adriaenssens, S. Wathlet, I. Segers, G. Verheyen, H. Van De Velde, W. Coucke, J. Smitz, G. Paternot, T. M. D'Hooghe, S. Debrock, C. Spiessens, H. K. Hwang, H. M. Kim, J. H. Lee, Y. J. Jung, A. Kang, M. J. Kook, J. Y. Jung, S. J. An, H. C. Kwon, S. J. Lee, O. Somova, A. Feskov, I. Feskova, N. Chumakova, O. Zozulina, Y. E. Zhilkova, M. Binda, R. Campo, G. Van Kerkhoven, V. Frederickx, A. Serneels, P. Roziers, I. Vranken, A. S. Lopes, A. Van Nuland, S. Gordts, P. Puttemans, M. Valkenburg, A. Rodriguez-Arnedo, J. Ten, J. Guerrero, B. Lledo, M. A. Carracedo, J. A. Ortiz, J. Llacer, R. Bernabeu, K. Usui, Y. Nakajo, M. Ota, H. Hattori, T. Kyoya, T. Takisawa, K. Kyono, A. Ferrieres, M. Poulain, V. Loup, T. Anahory, H. Dechaud, S. Hamamah, J. Eckert, G. Premkumar, F. Lock, S. Brooks, S. Haque, I. T. Cameron, Y. Cheong, T. P. Fleming, N. Prados, M. Ruiz, J. Garcia-Ortega, P. Vime, M. J. Hernaez, M. Crespo, M. Fernandez-Sanchez, S. Hashimoto, N. Kato, K. Saeki, Y. Morimoto, C. O. N. Leung, R. T. K. Pang, W. M. Liu, K. F. Lee, W. S. B. Yeung, T. Wada, T. Elliott, J. Kahn, J. Lowderman, G. Wright, C. Chang, D. Bernal, H. Kort, Z. Nagy, J. M. de los Santos, L. Escrich, N. Grau, M. J. Escriba, M. Escriba, F. Tasker, H. Hamoda, H. Wilner, J. Grace, Y. Khalaf, S. Miyaji, S. Mizuno, L. Horiuchi, A. Haruki, A. Fukuda, T. Utsunomiya, Y. Kumasako, H. Ito, K. Goto, M. Koike, H. Abe, T. Sakamoto, F. Kojima, T. Koshika, L. Muzii, M. C. Magli, L. Gioia, G. Scaravelli, A. P. Ferraretti, L. Gianaroli, A. Capoti, M. Lappi, E. Maggi, L. Scott, A. Finn, B. Kloos, D. Davies, M. Yamada, T. Hamatani, H. Akutsu, N. Chikazawa, S. Ogawa, N. Okumura, Y. Mochimaru, N. Kuji, D. Aoki, Y. Yoshimura, A. Umezawa, V. P. Aprysko, S. A. Yakovenko, E. A. Seregina, E. V. Yutkin, H. Yelke, S. Milik, Z. N. Candan, G. Altin, S. Unal, Z. Atayurt, null Y. Kumtepe, J. T. Chung, W. Y. Son, X. Zhang, S. L. Tan, A. Ao, E. Seli, L. Botros, M. Henson, P. Roos, K. Judge, D. Sakkas, M. S. G. M. S. group, M. Feliciano, D. Monahan, E. Ermolovich, Z. Rosenwaks, G. D. Palermo, E. Mantikou, J. van Echten-Arends, B. Sikkema-Raddatz, F. van der Veen, S. Repping, S. Mastenbroek, M. B. S. Group, V. Wells, M. Y. Thum, H. I. Abdalla, R. Machiya, S. Akimoto, T. Nobuyoshi, N. Yoshii, T. Hosaka, Y. Odawara, F. Vanden Meerschaut, S. Lierman, T. O'Leary, S. Assou, D. Haouzi, F. Pellestor, C. Monzo, J. De Vos, J. Conaghan, E. Fischer, J. Popwell, I. Ryan, P. Chenette, C. Givens, E. Schriock, C. Herbert, Q. V. Neri, M. Camus, P. Haentjens, A. Mugica, M. Esbert, J. M. Molina, N. Garrido, A. Ballesteros, G. Calderon, A. L. S. Rossi, A. M. Rocha, J. R. Alegretti, P. A. Hassun, L. P. Gomes, T. Criscuollo, P. Serafini, E. L. A. Motta, M. Munoz, M. Meseguer, M. Cruz, I. Perez-Cano, B. Gadea, M. Martinez, S. Fortuno, J. Gundersen, E. Selles, J. Betersen, E. Le Meaux, G. Ouandaogo, null S. Hamamah, E. Gismano, I. Cino, F. Calzi, E. Rabellotti, E. Papaleo, S. K. Sunkara, A. Siozos, V. Bolton, P. Braude, T. El-Toukhy, Y. S. Cho, B. Ambruosi, P. Totaro, M. E. Dell'Aquila, G. Gioacchini, D. Bizzaro, E. Giorgini, P. Ferraris, S. Sabbatini, O. Carnevali, P. Knaggs, A. Chau, S. Khalil, G. Trew, S. Lavery, V. P. Jovanovic, R. Gomez, C. M. Sauer, C. J. Shawber, H. H. Outtz, X. Wang, M. V. Sauer, J. Kitajewski, R. C. Zimmermann, E. Mahrous, H. Clarke, I. Virant-Klun, L. Bacer-Kermavner, J. Mivsek, T. Tomazevic, B. Pozlep, B. Zorn, E. Vrtacnik-Bokal, I. Dundure, J. Bazarova, V. Fodina, J. Brikune, J. Lakutins, B. Jee, J. Jo, J. Lee, C. Suh, S. Kim, S. Moon, Y. Shufaro, M. Lebovich, E. Aizenman, A. Simon, N. Laufer, null A. Saada Reisch, M. A. Ribeiro, A. Pinto, F. Gomes, J. L. Silva Carvalho, H. Almeida, F. C. Massaro, C. G. Petersen, A. L. Mauri, L. F. I. Silva, A. P. M. Nicoletti, M. Cavagna, A. Pontes, R. L. R. Baruffi, J. B. A. Oliveira, J. G. Franco, A. Valcarcel, M. I. Viglierchio, M. Tiveron, M. Guidobono, R. Inza, M. Vilela, A. Kenny, C. Lombardi, and G. Marconi

Subjects
biology, business.industry, Rehabilitation, Embryogenesis, Seven in absentia homolog, Obstetrics and Gynecology, SIAH1, Cell biology, Ubiquitin ligase, Reproductive Medicine, Immunology, microRNA, biology.protein, Medicine, business
Published
2010
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12. Naloxone decreases the inhibitory effect of ethanol on the release of arginine-vasopressin induced by physical exercise in man

Author

Gloria Saccani-Jotti, Paolo Chiodera, P. Vacca, Andrea Melani, P. Rubino, Elio Volta, M. L. Maffei, Vittorio Coiro, Riccardo Volpi, and A. Casti

Subjects
Adult, Male, Vasopressin, medicine.medical_specialty, Time Factors, Arginine, Narcotic Antagonists, Blood Pressure, Physical exercise, (+)-Naloxone, Inhibitory postsynaptic potential, Young Adult, chemistry.chemical_compound, Basal (phylogenetics), Oxygen Consumption, Heart Rate, Internal medicine, Tidal Volume, Humans, Medicine, Exercise, Biological Psychiatry, Endogenous opioid, Ethanol, Dose-Response Relationship, Drug, Naloxone, business.industry, Respiration, Central Nervous System Depressants, Carbon Dioxide, Arginine Vasopressin, Psychiatry and Mental health, Endocrinology, Gene Expression Regulation, Neurology, chemistry, Neurology (clinical), Pulmonary Ventilation, business, hormones, hormone substitutes, and hormone antagonists
Abstract

To establish whether ethanol and/or endogenous opioids play a role in the control of arginine-vasopressin (AVP) response to physical exercise, six healthy men underwent six bicycle-ergometer tests until exhaustion [exercise control test; exercise plus ethanol (50 of 110 ml proof whiskey orally), exercise plus naloxone (2 mg injected plus 5 mg infused or 4 mg injected plus 10 mg infused intravenously] or exercise plus ethanol plus naloxone). Plasma AVP levels, physiological and biochemical variables were measured during tests. Physiological and biochemical variables were similar in all tests. During the control test, exercise significantly increased plasma AVP levels, with a peak value five times higher than baseline. The AVP response to exercise was similar in the presence of naloxone, whereas it was abolished by ethanol. When ethanol tests were repeated in the presence of naloxone, at both lower and higher dose, ethanol inhibition on AVP secretion was only partial, with mean peak responses 2.5 times higher than basal values. Results indicate an ethanol involvement in regulation of the AVP response to physical exercise. Furthermore, naloxone-sensitive endogenous opioids appear to play a role in the mechanism underlying ethanol inhibitory action, but not in mediation of the AVP response to physical exercise.

Published
2009
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13. Effect of naloxone on somatostatin inhibition of arginine vasopressin response to physical exercise in normal men

Author

Simona Cataldo, P. Rubino, Guido Manfredi, Paolo Chiodera, Vittorio Coiro, G. Saccani Jotti, M. L. Maffei, Andrea Melani, A. Casti, and Elio Volta

Subjects
Adult, Blood Glucose, Male, endocrine system, Vasopressin, medicine.medical_specialty, Arginine, Narcotic Antagonists, Physical exercise, (+)-Naloxone, Inhibitory postsynaptic potential, Internal medicine, medicine, Humans, Drug Interactions, Exercise, Biological Psychiatry, Endogenous opioid, Naloxone, urogenital system, business.industry, Osmolar Concentration, Up-Regulation, Arginine Vasopressin, Psychiatry and Mental health, Endocrinology, Somatostatin, Opioid Peptides, Neurology, Physical Fitness, Exercise Test, Neurology (clinical), Peak value, business, hormones, hormone substitutes, and hormone antagonists
Abstract

To establish whether somatostatin (SRIH) and/or endogenous opioids play a role in the control of arginine-vasopressin (AVP) response to physical exercise, eight healthy men underwent four bicycle-ergometer tests until exhaustion: exercise control test; exercise plus SRIH, naloxone or SRIH plus naloxone. Serum AVP levels, physiological and biochemical variables were measured during tests. Physiological and biochemical variables were similar in all tests. During control test exercise significantly increased serum AVP levels, with a peak value 4.1 times higher than baseline. The AVP response to exercise was similar in the presence of naloxone, whereas it was significantly reduced by SRIH (AVP peak was only 2.8 times higher than baseline). When SRIH and naloxone were given together, the exercise-induced AVP rise was comparable to that observed in the control test. Results indicate a somatostatinergic involvement in the regulation of the AVP response to physical exercise. Furthermore, naloxone-sensitive endogenous opioids appear to play a role in the mechanism underlying SRIH inhibitory action, but not in mediation of the AVP response to physical exercise.

Published
2008
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14. Adrenocorticotropic Hormone/Cortisol Response to Physical Exercise in Abstinent Alcoholic Patients

Author

Paolo Chiodera, Elio Volta, M. L. Maffei, Guido Manfredi, Gloria Saccani Jotti, A. Casti, Vittorio Coiro, P. Rubino, and Andrea Melani

Subjects
Adult, Blood Glucose, Male, Hypothalamo-Hypophyseal System, endocrine system, medicine.medical_specialty, Hydrocortisone, Temperance, media_common.quotation_subject, Immunoglobulins, Medicine (miscellaneous), Physical exercise, Adrenocorticotropic hormone, Fatty Acids, Nonesterified, Toxicology, Oxygen Consumption, Adrenocorticotropic Hormone, Liver Function Tests, Internal medicine, Heart rate, medicine, Humans, Lactic Acid, Exercise, Respiratory exchange ratio, media_common, Psychiatric Status Rating Scales, Pulmonary Gas Exchange, Carbon Dioxide, Middle Aged, Abstinence, Alcoholism, Psychiatry and Mental health, medicine.anatomical_structure, Endocrinology, Psychology, hormones, hormone substitutes, and hormone antagonists, Glucocorticoid, Hypothalamic–pituitary–adrenal axis, medicine.drug
Abstract

Background: Alterations in the hypothalamic–pituitary–adrenal (HPA) axis in alcoholic patients have been reported in various experimental conditions. Methods: To establish whether alcoholism affects the HPA axis activation during physical exercise, 10 recent abstinent alcoholic patients (age range: 33–45 years; duration of alcohol dependence: range 4–6 years) were tested by exercising on a bicycle ergometer. Ten age-matched healthy nonalcoholic men participated as controls. The workload was gradually increased at 3-minute intervals until exhaustion and lasted about 15 minutes for all subjects. Alcoholic patients were tested at 3 time points, at 4, 6, and 8 weeks after alcohol withdrawal, whereas controls were tested only once. Main outcome measurements were circulating levels of adrenocorticotropic hormone (ACTH) and cortisol and physiological variables during physical exercise [heart rate, blood pressure, ventilation, frequency of breathing, tidal volume, oxygen consumption (VO2), carbon oxide production (VCO2), and respiratory exchange ratio (R)]. Results: Similar basal and exercise-induced changes in physiological variables were observed in controls and alcoholic patients in all tests. Basal levels of ACTH and cortisol were similar in all tests performed on alcoholic patients and on normal controls. In normal subjects, exercise induced a significant increase in plasma ACTH and serum cortisol levels, with peak levels at 20 minutes for ACTH (84% higher than baseline) and at 30 minutes for cortisol (70% higher than baseline). After 4 weeks of abstinence, slight but not significant ACTH/cortisol responses to physical exercise were observed in alcoholic patients (mean peaks were 10 and 18% higher than baseline, respectively, for ACTH and cortisol). By contrast, when the exercise test was repeated after 6 weeks abstinence, ACTH/cortisol levels rose significantly versus baseline (mean peak levels of ACTH and cortisol were 48 and 38% higher than baseline, respectively, for ACTH and cortisol). However, the hormonal responses were significantly lower than in the normal controls. At 8 weeks of abstinence, ACTH/cortisol responses were significantly higher than 2 weeks previously, and were not distinguishable from the increments observed in the normal controls (76 and 68% higher than baseline, respectively, for ACTH and cortisol). Conclusions: In concurrence with previous reports showing alterations of the HPA axis in the central nervous system in alcohol-dependent subjects, these data show a defect of the neuroendocrine mechanism(s) underlying the ACTH/cortisol response to physical exercise for at least a month after alcohol withdrawal, with reconstitution of a normal hormonal response at 8 weeks.

Published
2007
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15. Successful implantation and live birth of a healthy boy after triple biopsy and double vitrification of oocyte-embryo-blastocyst

Author

Rocio P. Cotarelo, P. Rubino, Francesco Fiorentino, Ermanno Greco, Jan Tesarik, Maria Giulia Minasi, Anil Biricik, and Elisabetta Iammarone

Subjects
Gynecology, medicine.medical_specialty, Multidisciplinary, medicine.diagnostic_test, Case Study, business.industry, urogenital system, Embryo, Blastomere, Preimplantation genetic diagnosis, Vitrification, Blastocyst biopsy, Cryopreservation, Andrology, Polar body, medicine.anatomical_structure, Biopsy, embryonic structures, Polar body biopsy, medicine, Blastocyst, Embryo biopsy, business, reproductive and urinary physiology
Abstract

Introduction Preimplantation genetic diagnosis and/or screening (PGD/PGS) allow the assessment of the genetic health of an embryo before transferring it into the uterus. These techniques require the removal of cellular material (polar bodies, blastomere(s) or trophectoderm cells) in order to perform the proper genetic analysis. We report the implantation and live birth outcome of a vitrified-warmed blastocyst developed after triple biopsy and double vitrification procedures at oocyte, cleavage embryo and blastocyst stage. Case description An infertile couple, with family history of β-thalassemia, searched for IVF procedure and PGD. First polar bodies biopsy with subsequent vitrification was uninformative due to meiotic crossing-over, so oocytes were inseminated after warming. Two embryos were obtained and blastomere biopsy was performed on day 3 with inconclusive results on their genetic status. Their culture resulted in one expanded blastocyst stage on day 7 that underwent trophectoderm biopsy and vitrification. This embryo showed to be normal. It was then warmed and transferred in an artificial cycle. Discussion and Evaluation Preconception genetic analysis by removal and analysis of the first polar body is technically possible, but the genetic information that we can obtain at this stage may be limited and the oocytes to be inseminated is not predictable. Compared to blastomere biopsy, trophectoderm biopsy has more diagnostic efficiency with respect to both chromosomal mosaicism and PCR accuracy, reducing the problems of amplification failure and allele drop out. Moreover, embryos biopsied at the cleavage stage seem to have lower implantation rate than biopsied blastocyst. Conclusions This is the first case report of a live birth obtained from a three step biopsy and double vitrification procedures of a blastocyst. This case report seems also to suggest the harmlessness of all these procedures if carefully performed by a skilled biologist in an IVF lab with quality management system. Finally, our study highlight that blastocyst cryopreserved on day 7 have clinically important potential and embryos that not reach blastocyst stage on day 6 should not to be discharged because they may result in an ongoing pregnancy.

Published
2015

16. Advanced paternal age does not affect embryo aneuploidy rate in egg donor cycles

Author

B. Kolb, J.M. Norian, T. Tan, J. Nelson, J. Wilcox, A. Thiel, R. Ruiz de Assin, P. Rubino, X. Li, and L. Tapia

Subjects
0301 basic medicine, Genetics, 030219 obstetrics & reproductive medicine, Obstetrics and Gynecology, Aneuploidy, Paternal age, Embryo, Biology, Affect (psychology), medicine.disease, Andrology, 03 medical and health sciences, Egg donation, 030104 developmental biology, 0302 clinical medicine, Reproductive Medicine, medicine
Published
2017
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17. SELECTED ORAL COMMUNICATION SESSION, SESSION 63: PREIMPLANTATION GENETICS Wednesday 6 July 2011 10:00 - 11:45

Author

P. Colls, J. Fischer, T. Escudero, K. Ketterson, G. Harton, S. Munne, A. Capalbo, F. Fiorentino, R. Maggiulli, S. Romano, A. Borsatti, A. Joseph, L. Spizzichino, S. Bono, A. Biricik, S. Colamaria, M. F. Ubaldi, L. F. Rienzi, P. Rubino, L. Arizzi, M. G. Minasi, R. Pena, F. Scarselli, V. Casciani, A. Colasante, S. Ferrero, K. Litwicka, M. T. Varricchio, F. Cucinelli, Z. P. Nagy, E. Greco, C. Beyazyurek, C. G. Ekmekci, H. A. Tac, N. Ajredin, H. Yelke, S. Kahraman, M. De Rademaeker, C. Moutou, M. Van Rij, J. Dreesen, M. De Rycke, I. Liebaers, S. Viville, J. Geraedts, C. De Die, D. Wells, E. Fragouli, S. Alfarawati, A. Kashevarova, E. Tolmacheva, N. Sukhanova, and I. Lebedev

Subjects
Medical education, Reproductive Medicine, business.industry, Rehabilitation, Obstetrics and Gynecology, Medicine, Session (computer science), business, Bioinformatics
Published
2011
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18. Improving fertilization rate in ICSI cycles by adding myoinositol to the semen preparation procedures: a prospective, bicentric, randomized trial on sibling oocytes

Author

Carlo Bulletti, Antonella Quagliariello, Silvia De Stefani, Andrea Baglioni, Gianfranco Carlomagno, Sara Chigioni, P. Rubino, and Simone Palini

Subjects
Infertility, Adult, Male, endocrine system, Pregnancy Rate, Semen, Fertilization in Vitro, law.invention, Male infertility, Andrology, Human fertilization, Randomized controlled trial, law, Pregnancy, Genetics, Medicine, Humans, Sperm Injections, Intracytoplasmic, Assisted Reproduction Technologies, reproductive and urinary physiology, Genetics (clinical), Infertility, Male, urogenital system, business.industry, Obstetrics and Gynecology, General Medicine, medicine.disease, Embryo Transfer, Spermatozoa, Embryo transfer, Pregnancy rate, Reproductive Medicine, Fertilization, embryonic structures, Oocytes, Female, business, therapeutics, Embryo quality, Inositol, Developmental Biology
Abstract

To evaluate whether the in vitro incubation of spermatozoa with myoinositol may improve the fertilization rate in ICSI cycles.This is a prospective, bicentric, randomized study on 500 MII sibling oocytes injected in 78 ICSI cycles performed between March and October 2013. Randomization of the oocytes into two groups was performed at the time of the denudation. Fertilization rates (per oocyte injected with spermatozoa treated with myoinositol versus per oocyte injected with spermatozoa treated with placebo) were measured as primary outcome and embryo morphology as secondary outcome. Clinical outcomes were also documented.Fertilization rate (78.9 ± 28.6% vs 63.2 ± 36.7, P = 0.002) and percentage of grade A embryos on day 3 (59.8 ± 35.6% vs 43.5 ± 41.5, P = 0.019) were significantly higher when spermatozoa were treated in vitro with myoinositol versus placebo. No differences were found for the expanded blastocyst formation rate.In vitro treatment of spermatozoa with myoinositol may optimize ICSI outcomes by improving the fertilization rate and embryo quality on day 3. The improvement of the number and the quality of embryos available in an ICSI cycle may have clinical utility if these findings can be confirmed.

Published
2014

19. Oxytocin inhibits the stimulatory effect of ghrelin on circulating neuropeptide Y levels in humans

Author

Vittorio Coiro, Gloria Saccani-Jotti, Paolo Chiodera, P. Rubino, Paola Vacca, Elio Volta, and Guido Manfredi

Subjects
Adult, Male, medicine.medical_specialty, medicine.medical_treatment, Oxytocin, Body weight, Intravenous bolus, Basal (phylogenetics), Internal medicine, mental disorders, medicine, Humans, Drug Interactions, Neuropeptide Y, Secretion, Saline, Biological Psychiatry, Chemistry, digestive, oral, and skin physiology, Neuropeptide Y receptor, Ghrelin, humanities, Up-Regulation, Psychiatry and Mental health, Endocrinology, Neurology, Injections, Intravenous, Neurology (clinical), hormones, hormone substitutes, and hormone antagonists, medicine.drug
Abstract

Oxytocin (OT) effect on ghrelin-stimulated neuropeptide Y (NPY) secretion was evaluated in 12 normal men. Tests: ghrelin (1 μg/kg B.W. as an intravenous bolus); OT (2 mIU/min infusion); ghrelin plus OT; normal saline. Plasma NPY did not change during saline or OT infusions, whereas it showed a significant 29% increase vs baseline at 15 min after ghrelin injection. When OT was present, ghrelin-induced NPY increment was completely abolished. Results show that oxytocin modulates the NPY response to ghrelin, whereas it is unable to produce direct inhibitions of basal circulating NPY levels.

Published
2008
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20. Abstracts of the 12th European Colloquium on Cytogenetics of Domestic Animals

Author

M. MacDougall, Alexander S. Graphodatsky, George Klein, I. Ohkubo, E. Van Hul, T.A. Jones, T.B. Shows, H. Nishino, D. Simmons, M.R. Martorell, L.A. Cannizzaro, M.R. Ribaudo, N. Nowak, M. Noble, V.S. Lestou, H.H.Q. Heng, C. Márquez, Vladimir I. Kashuba, A.I. Protopopov, J. Merregaert, C. Templado, G. Novelli, F. Sangiuolo, Keith Johnson, G. Calabrese, A.M. Ruzzo, P. Rubino, Harvey Mohrenweiser, P. Colls, I.H. Still, S. Feo, T.H. Chu, B.R. DuPont, J. Cowell, Y. Yonenaga-Yassuda, J. Inazawa, R.J. Leach, W. Van Hul, N.J. Gutowski, S. Munné, Tomio Miwa, M.T. Rodrigues, J. Williamson, C. Pröschel, M. Magnani, A.M. Estop, N.V. Vorobieva, R. Z. Gizatullin, P.F. Ambros, H. Gadner, A. Di Leonardo, P.J. Willems, B. Dallapiccola, K. Cieply, M. Gennarelli, A. Giallongo, Denise Sheer, J. Benet, X. Mao, J. Wauters, L. Mori, Eugene R. Zabarovsky, M.V. Protopopova, H. Ueyama, A. Colosimo, Y. Xie, J. Navarro, T. Lion, S. Strehl, V. Van Kirk, G. Hong, and G. Palka

Subjects
Genetics, medicine.medical_specialty, Evolutionary biology, Cytogenetics, medicine, Biology, Molecular Biology, Genetics (clinical)
Published
1996
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21. Trophectoderm biopsy performed by a double laser zona drilling: first safety evidence

Author

P. Rubino, Alessandra Alteri, Pierluigi Giannini, Emilia Rega, Paola Viganò, and Rocio P. Cotarelo

Subjects
medicine.medical_specialty, business.industry, Biopsy, Lasers, Obstetrics and Gynecology, Aneuploidy, Laser, law.invention, Zona drilling, Surgery, Blastocyst, Reproductive Medicine, law, Blastocyst Inner Cell Mass, Humans, Medicine, Genetic Testing, business, Zona Pellucida, Trophectoderm biopsy
Published
2014
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22. Effect of physical training on reduction of circulating neuropeptide Y levels in elderly humans

Author

Elio Volta, Andrea Melani, Paolo Chiodera, Gloria Saccani-Jotti, P. Rubino, A. Casti, Riccardo Volpi, and Vittorio Coiro

Subjects
Adult, Male, medicine.medical_specialty, Aging, business.industry, Endocrinology, Diabetes and Metabolism, Middle Aged, Neuropeptide Y receptor, Body Mass Index, Running, Reduction (complexity), Endocrinology, Internal medicine, medicine, Humans, Neuropeptide Y, business, Body mass index, Exercise, Aged
Published
2009

23. Free fatty acids inhibit adrenocorticotropin and cortisol secretion stimulated by physical exercise in normal men

Author

P. Rubino, G. Saccani Jotti, A. Casti, M. L. Maffei, Andrea Melani, Guido Manfredi, Paolo Chiodera, and Vittorio Coiro

Subjects
Cortisol secretion, Adult, Male, endocrine system, medicine.medical_specialty, Hydrocortisone, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Physical exercise, Adrenocorticotropic hormone, Fatty Acids, Nonesterified, Basal (phylogenetics), Endocrinology, Adrenocorticotropic Hormone, Internal medicine, medicine, Humans, Infusions, Intravenous, Saline, Cortisol level, Exercise, chemistry.chemical_classification, Analysis of Variance, business.industry, Heparin, Fatty acid, chemistry, Depression, Chemical, Bicycle ergometer, business, hormones, hormone substitutes, and hormone antagonists
Abstract

Summary Background The basal circulating levels of ACTH and cortisol, but not the ACTH/cortisol response to hCRH, are significantly reduced by free fatty acid (FFA) infusion. Objective To verify whether FFA infusion modifies the ACTH/cortisol response to physical exercise, a well-known activator of the HPA axis at suprapituitary level. Design Exercise tests on a bicycle ergometer during infusion of a lipid-heparin emulsion (LHE) (experimental test) or normal saline (NaCl 0·9%) (control test). Setting Department of Cardiology at the University-Hospital. Subjects Seven healthy male subjects aged 25–33 years. Interventions On two mornings, at weekly intervals, LHE or saline were infused for 60 min; infusion started 10 min before exercise test on a bicycle ergometer, which lasted about 15 min. Main outcome measures Circulating ACTH/cortisol levels and physiological variables during physical exercise. Results FFA levels (0·4 ± 0·1 mEq/l) remained constant during control test, whereas they progressively rose (peak at 60 min, 2·7 ± 1·0 mEq/l) during LHE infusion. Neither basal nor exercise-induced changes in physiological variables were modified by LHE infusion. Both ACTH and cortisol increased during exercise, with peak levels at 20 min and 30 min (control test: 103% and 42%, P

Published
2007

24. The ICSI procedure from past to future: a systematic review of the more controversial aspects

Author

Paola Piomboni, Paola Viganò, Alice Luddi, and P. Rubino

Subjects
Male, medicine.medical_specialty, Microinjections, medicine.medical_treatment, DNA Fragmentation, Asthenozoospermia, ICSI, Miscarriage, Andrology, Pregnancy, Metaphase I oocyte, Humans, Medicine, Sperm Injections, Intracytoplasmic, Intensive care medicine, Metaphase, reproductive and urinary physiology, Assisted reproductive technology, urogenital system, business.industry, Fertilization failure, Obstetrics and Gynecology, Immotile sperm, Zona-free oocyte, medicine.disease, Spermatozoa, Sperm, Reproductive Medicine, Fertilization, Oocytes, Oocyte activation, Female, Metaphase i, Female gametes, Globozoospermia, business, Live birth
Abstract

Background ICSI is currently the most commonly used assisted reproductive technology, accounting for 70-80% of the cycles performed. This extensive use, even excessive, is partly due to the high level of standardization reached by the procedure. There are, however, some aspects that deserve attention and can still be ameliorated. The aim of this systematic review was to evaluate the results of available publications dealing with the management of specific situations during ICSI in order to support embryologists in trying to offer the best laboratory individualized treatment. Methods This systematic review is based on material obtained by searching PUBMED between January 1996 and March 2015. We included peer-reviewed, English-language journal articles that have evaluated ICSI outcomes in the case of (i) immature oocytes, (ii) oocyte degeneration, (iii) timing of the various phases, (iv) polar body position during injection, (v) zona-free oocytes, (vi) fertilization deficiency, (vii) round-headed sperm, (viii) immotile sperm and (ix) semen samples with high DNA fragmentation. Results More than 1770 articles were obtained, from which only 90 were specifically related to the issues developed for female gametes and 55 for the issues developed for male gametes. The studies selected for this review were organized in order to provide a guide to overcome roadblocks. According to these studies, the injection of rescue metaphase I oocytes should be discouraged due to poor clinical outcomes and a high aneuploidy rates; laser-assisted ICSI represents an efficient method to solve the high oocyte degeneration rate; the optimal ICSI timing and the best polar body position during the injection have not been clarified; injected zona-free oocytes, if handled carefully, can develop up to blastocyst stage and implant; efficient options can be offered to patients who suffered fertilization failure in previous conventional ICSI cycles. Most controversial and inconclusive are data on the best method to select a viable spermatozoa when only immotile spermatozoa are available for ICSI and, to date, there is no reliable approach to completely filter out spermatozoa with fragmented DNA from an ejaculate. However, most of the studies do not report essential clinical outcomes, such as live birth, miscarriage and fetal abnormality rate, which are essential to establish the safety of a procedure. Conclusions This review provides the current knowledge on some controversial technical aspects of the ICSI procedures in order to improve its efficacy in specific contexts. Notwithstanding that embryologists might benefit from the approaches presented herein in order to improve ICSI outcomes, this area of expertise still demands a greater number of well-designed studies, especially in order to solve open issues about the safety of these procedures.

Published
2015
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25. Improving ICSI outcomes by adding myo-inositol to the semen preparation procedures: a prospective, randomized sigle blind trial on sibling-oocyte

Author

G. Carlomagno, A. Quagliariello, A.G. Baglioni, P. Rubino, S. Chigioni, and G.M. Galofre Ballesteros

Subjects
Ejaculated spermatozoa, business.industry, Obstetrics and Gynecology, Semen, Oocyte, law.invention, Andrology, Human fertilization, medicine.anatomical_structure, Reproductive Medicine, Randomized controlled trial, law, Medicine, Sibling, business, Preparation procedures, Embryo quality
Abstract

OBJECTIVE: The aim of the study was to evaluate whether myo-inositol (MI) treatment during semen preparation procedures is able to improve fertilization rate (FR) and embryo quality (EQ) after ICSI. DESIGN: The study was designed as a prospective randomized single blind controlled trial on sibling-oocyte and was performed from March to December 2013 (NCT02050672). Before recruiting, the sample size was calculated via a power analysis and the FR was designed as primary outcome. In particular, based on a difference of 15% on the FR, and power of 90% and confidence of 95%, a minimum of 210 oocytes per group were required. MATERIALS AND METHODS: Inclusion criteria: 1)couple counseled for ICSI due to male factor; 2) fresh cycles 3) men with ejaculated spermatozoa; 3) women age of >35 yrs; 4) R2 MII oocyte retrieved. At ovum pick up, oocytes were randomly divided in two groups. Mature oocytes of one group (MI) were injected with spermatozoa prepared with routinely used media enriched with 2mg/ml of MI (Andrositol LAB Lo.Li. Pharma), mature oocytes of the other group (CTR) were injected with spermatozoa prepared with routinely used media. Secondary outcome was the EQ. A blinded embryologist performed ICSI procedure and embryo evaluation. RESULTS: In summary, FR cleavage rate (CR) and EQ were significantly higher in MI group. Furthermore, significantly more embryos of the MI group were transferred at day 3; noteworthy, the blind researcher has chosen to transfer only embryos belonging to the MI group with a significant higher frequency.

Published
2014
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26. Concentration of motile spermatozoa retrieved after sperm washing treatment does not affect clinical outcome of HIV discordant ICSI cycle

Author

A. Quagliariello, P. Rubino, S. Chigioni, G.M. Galofre Ballesteros, A. Vucetich, and E.A. Semprini

Subjects
Andrology, Reproductive Medicine, business.industry, Human immunodeficiency virus (HIV), medicine, Sperm washing, Obstetrics and Gynecology, medicine.disease_cause, business, Affect (psychology), Motile spermatozoa
Published
2013
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28. CORONARY INTRASTENT RESTENOSIS AND BLOOD PRESSURE LEVELS: RETROSPECTIVE ANALYSIS OF A LARGE COHORT OF PATIENTS WITH CORONARY SINGLE VESSEL DISEASE: 1C.06

Author

Andrea Ferrucci, A Saponaro, Domenico Maria Zardi, G. Sorropaco, M. Volpe, B.A. Pace, Anna Modestino, Andrea Berni, Roberta Coluccia, P. Rubino, Giuliano Tocci, Sofia Abbolito, and C. Nannini

Subjects
medicine.medical_specialty, Physiology, business.industry, Single vessel, Disease, medicine.disease, Large cohort, Blood pressure, Restenosis, Internal medicine, Internal Medicine, Cardiology, Retrospective analysis, Medicine, Cardiology and Cardiovascular Medicine, business
Published
2010
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29. Incidence and risk factors of acute kidney injury in redo cardiac surgery: a single center analysis

Author

Luca Salvatore De Santo, Antonino Salvatore Rubino, Antonio Pio Montella, Caterina Golini Petrarcone, Lucrezia Palmieri, Denise Galbiati, Antonio Pisano, and Marisa De Feo

Subjects
Medicine, Science
Abstract

Abstract The incidence, risk factors and prognostic implications of acute kidney injury (AKI) in patients undergoing redo cardiac surgery are still poorly defined. We prospectively collected data on 394 consecutive redo patients between January 2011 and October 2020. Patients were divided into groups according to the occurrence of different degrees of postoperative AKI (No AKI vs. Any AKI; No AKI-AKI 1 vs. AKI 2–3). The relationship between AKI and other major complications was also investigated. Postoperatively, AKI 1 occurred in 124 (31.5%), AKI 2 in 36 (9.1%) and AKI 3 in 64 (16.2%). Higher KDIGO classes were associated with increased in-hospital mortality: 5.3% among patients with no postoperative AKI and 8.9%, 13.9% and 64.1% in patients with AKI 1, 2 and 3, respectively (p

Published
2024
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30. Resistive index of central retinal artery, aortic arterial stiffness and OCTA correlated parameters in the early stage of fabry disease

Author

Michele Rinaldi, Flavia Chiosi, Maria Laura Passaro, Francesco Natale, Alessia Riccardo, Luca D’Andrea, Martina Caiazza, Marta Rubino, Emanuele Monda, Gilda Cennamo, Francesco Calabrò, Giuseppe Limongelli, and Ciro Costagliola

Subjects
Fabry disease, Retinal vessel density, Retinal and choroidal microangiopathy, OCT angiography, Resistive index of central retinal artery, Medicine, Science
Abstract

Abstract This study aimed to evaluate the impact of Fabry disease (FD) on retinal microvasculature using optical coherence tomography angiography (OCTA), arterial stiffness, and the resistive index (RI) of the central retinal artery (CRA) in early disease stages. Twenty-nine genetically confirmed FD patients and twenty-six healthy controls were enrolled. Vessel density (VD) values of the superficial, deep, and choriocapillaris plexuses (SCP, DCP, and CC) were measured via OCTA. CRA RI was studied using color Doppler and grayscale sonography, and aortic pulse wave velocity (PWV) was assessed with the Complior method. CRA RI was significantly lower in the control group compared to the Fabry group (p

Published
2024
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31. Cyclic carbamates based on (R)-(+)-limonene oxide for ring-opening polymerization

Author

Lucia Rubino, Vincenzo Patamia, Antonio Rescifina, Maurizio Galimberti, and Vincenzina Barbera

Subjects
Isocyanate free, Dimethyl carbonate, Sustainable, Polyurethane, Density functional theory, Medicine, Science
Abstract

Abstract A novel synthetic pathway for synthesizing isocyanate-free polyurethanes is reported here. β-Amino alcohols were efficiently synthesized from the aminolysis of the epoxide ring of (R)-(+)-limonene oxide with different primary amines as nucleophiles and hot water as catalysts. The regio- and diastereoselectivities of the reactions were investigated and supported by computational studies. DFT calculations were performed to understand the experimental results more deeply. It confirmed the crucial roles of water molecules and the nature of the nucleophile in forming the products. The formation of the product is entirely driven by the free energy of activation that affects the reaction rate. Cyclic carbamates were prepared from β-amino alcohols using the dialkyl carbonate (DAC) chemistry. An oligourethane was obtained from Anionic Ring-Opening Polymerization (AROP) of a cyclic carbamate derived from (R)-(+)-limonene-oxide. All the products were characterized by employing 1H and 13C NMR spectroscopies. The assignments of the signals in 1H and 13C NMR spectra were also supported by 2D NMR spectroscopy.

Published
2024
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32. Experimentelle Arteriosklerose im Verlaufe der Hypervitaminose D bei Kaninchen und Ratten

Author

P. Rubino, J. A. Collazo, J. Moreau, and B. Varela

Subjects
Gynecology, medicine.medical_specialty, Chemistry, medicine, Cell Biology, General Medicine, Molecular Biology, Pathology and Forensic Medicine
Abstract

1. Weise Ratten und Kaninchen, die grose Mengen (3–5 mg) von bestrahltem Ergosterin taglich erhalten, gehen nach 20–30 Tagen zugrunde Die grosen Arterien sind von einem akuten arteriosklerotischen Prozes befallen. 2. Es handelt sich um eine spezifische Arteriosklerose, die bemerkenswerte Unterschiede mit anderen, schon bekannten experimentellen Arteriosklerosen (Adrenalin, alimentare Cholesterinsklerose) aufweist. 3. Diese akute Arteriosklerose bei der Hypervitaminose D zeichnet sich durch einen der menschlichen Arteriosklerose sehr ahnlichen Anfang aus. In ihrem weiteren Verlauf aber last sich ein verschiedenartiges Verhalten erkennen, das darin besteht, das bei der Hypervitaminose D-Arteriosklerose eine ausgesprochene Neigung zur Verkalkung in dem letzten Stadium vorherrscht.

Published
1929
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35. Relationship between adherence to bictegravir/emtricitabine/tenofovir alafenamide fumarate and clinical outcomes in people with HIV in Japan: a claims database analysis

Author

Nao Taguchi, Yi Piao, Annalisa Rubino, KuanYeh Lee, Megan Chen, Keisuke Harada, Tetsuya Tanikawa, and Toshio Naito

Subjects
Medicine, Science
Abstract

Abstract A lack of adherence to long-term antiretroviral therapy may impact viral suppression. The current study examined the relationship between medication adherence and clinical outcomes in people with human immunodeficiency virus infection (PWH) receiving bictegravir, emtricitabine, and tenofovir alafenamide fumarate (B/F/TAF). A retrospective cohort study using two Japanese claims databases was conducted. Adherence was measured by the proportion of days covered (PDC). Patients were grouped into 3 PDC category and persistence was estimated by Kaplan-Meier method. Cox regression analysis was performed to investigate whether the PDC was associated with treatment discontinuation. Among 952 patients, 820 (86.1%), 95 (10.0%), and 37 (3.9%) patients were grouped into the PDC ≥ 90%, 80–

Published
2024
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37. Hypervitaminose D

Author

P. Rubino, B. Varela, and J. A. Collazo

Subjects
medicine.medical_specialty, Pathology, Endocrinology, business.industry, Internal medicine, medicine, Vitamin D and neurology, Histology, General Medicine, business, Hypervitaminosis, medicine.disease
Published
1929
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38. Two Cases of Rat-Bite Fever

Author

Anthony P. Rubino

Subjects
relapsing fever, business.industry, Rat-bite fever, Immunology, General Engineering, medicine, medicine.disease, business
Published
1927
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39. Comparison of HPV-positive triage strategies combining extended genotyping with cytology or p16/ki67 dual staining in the Italian NTCC2 studyResearch in context

Author

Maria Benevolo, Guglielmo Ronco, Pamela Mancuso, Francesca Carozzi, Laura De Marco, Elena Allia, Simonetta Bisanzi, Raffaella Rizzolo, Daniela Gustinucci, Annarosa Del Mistro, Helena Frayle, Massimo Confortini, Jessica Viti, Anna Iossa, Elena Cesarini, Simonetta Bulletti, Basilio Passamonti, Silvia Gori, Laura Toniolo, Laura Bonvicini, Francesco Venturelli, Nicolas Wentzensen, Paolo Giorgi Rossi, Alessandra Barca, Francesco Quadrino, Francesca Rollo, Gabriele Carlinfante, Teresa Rubino, Francesca Maria Carozzi, Cristina Sani, Andrea Baldini, Giampaolo Pompeo, Alessandra Mongia, Giulia Fantacci, Donella Puliti, Carmelina Di Pierro, Luigia Macrì, Teresa Pusiol, Mattia Barbareschi, Emma Bragantini, Gabriella Penon, Natalina Marchi, Manuel Zorzi, Elena Narne, and Anna Turrin

Subjects
Human papillomavirus, HPV genotyping, Cervical cancer screening, Triage, HPV DNA testing, Accuracy, Medicine, Medicine (General), R5-920
Abstract

Summary: Background: Each high-risk HPV genotype has different oncogenic potential, and the risk of CIN3+ varies according to genotype. We evaluated the performance of different strategies of HPV-positivity triage combining cytology, p16/ki67 dual staining (DS), and extended genotyping. Methods: Samples from 3180 consecutive women from the NTCC2 study (NCT01837693) positive for HPV DNA at primary screening, were retrospectively analyzed by the BD Onclarity HPV Assay, which allows extended genotyping. Genotypes were divided into three groups based on the risk of CIN3+. HPV DNA-positive women were followed up for 24 months or to clearance. Findings: Combining the three groups of genotypes with cytology or DS results we identify a group of women who need immediate colposcopy (PPV for CIN3+ from 7.8 to 20.1%), a group that can be referred to 1-year HPV retesting (PPV in those HPV-positive at retesting from 2.2 to 3.8), and a group with a very low 24-month CIN3+ risk, i.e. 0.4%, composed by women cytology or DS negative and positive for HPV 56/59/66 or 35/39/68 or negative with the Onclarity test, who can be referred to 3-year retesting. Interpretation: Among the baseline HPV DNA positive/cytology or DS negative women, the extended genotyping allows to stratify for risk of CIN3+, and to identify a group of women with a risk of CIN3+ so low in the next 24 months that they could be referred to a new screening round after 3 years. Funding: Italian Ministry of Health (grant number RF-2009-1536040). Hologic-Genprobe, Roche Diagnostics, and Becton & Dickinson provided financial and non-financial support.

Published
2024
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40. Investigating microRNAs as biomarkers in disorders of consciousness: a longitudinal multicenter study

Author

Nicolò Musso, Dalida Bivona, Carmelo Bonomo, Paolo Bonacci, Maria Enza D’Ippolito, Cristina Boccagni, Francesca Rubino, Antonio De Tanti, Lucia Francesca Lucca, Valeria Pingue, Valentina Colombo, Anna Estraneo, Stefania Stefani, Maria Andriolo, and Sergio Bagnato

Subjects
Medicine, Science
Abstract

Abstract MicroRNAs (miRNAs) are involved in gene regulation and may affect secondary brain injury and recovery in patients with disorders of consciousness (DoC). This study investigated the role of five miRNAs (150-5p, 132-3p, 23b-3p, 451a, and 16-5p) in prolonged DoC. miRNA levels were assessed in serum samples from 30 patients with unresponsive wakefulness syndrome or minimally conscious state due to traumatic or hypoxic-ischemic brain injury (TBI, HIBI) at baseline (1–3 months) and 6 months post-injury. Patients’ diagnoses were determined using the Coma Recovery Scale revised, and functional outcomes were evaluated 6 months after injury with the Glasgow Outcome Scale Extended (GOSE) and the Functional Independence Measure (FIM). Compared to healthy controls, patients with TBI had lower levels of miRNAs 150-5p, 132-3p, and 23b-3p at baseline, while patients with HIBI had lower levels of miRNA 150-5p at baseline and 6 months post-injury and a reduction of miRNA 451a at baseline. Higher levels of miRNAs 132-3p and 23b-3p were associated with better outcomes in TBI patients as indicated by GOSE and FIM scores. This study highlights distinct miRNA dysregulated patterns in patients with prolonged DoC, dependent on etiology and post-injury time, and suggests that miRNAs 132-3p and 23b-3p may serve as prognostic biomarkers.

Published
2023
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41. Improved processing speed and decreased functional connectivity in individuals with chronic stroke after paired exercise and motor training

Author

Justin W. Andrushko, Shie Rinat, Brian Greeley, Beverley C. Larssen, Christina B. Jones, Cristina Rubino, Ronan Denyer, Jennifer K. Ferris, Kristin L. Campbell, Jason L. Neva, and Lara A. Boyd

Subjects
Medicine, Science
Abstract

Abstract After stroke, impaired motor performance is linked to an increased demand for cognitive resources. Aerobic exercise improves cognitive function in neurologically intact populations and may be effective in altering cognitive function post-stroke. We sought to determine if high-intensity aerobic exercise paired with motor training in individuals with chronic stroke alters cognitive-motor function and functional connectivity between the dorsolateral prefrontal cortex (DLPFC), a key region for cognitive-motor processes, and the sensorimotor network. Twenty-five participants with chronic stroke were randomly assigned to exercise (n = 14; 66 ± 11 years; 4 females), or control (n = 11; 68 ± 8 years; 2 females) groups. Both groups performed 5-days of paretic upper limb motor training after either high-intensity aerobic exercise (3 intervals of 3 min each, total exercise duration of 23-min) or watching a documentary (control). Resting-state fMRI, and trail making test part A (TMT-A) and B were recorded pre- and post-intervention. Both groups showed implicit motor sequence learning (p

Published
2023
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42. Efficacy of microbicidal actives and formulations for inactivation of Lassa virus in suspension

Author

Todd A. Cutts, Raymond W. Nims, Joseph R. Rubino, Julie McKinney, Jens H. Kuhn, and M. Khalid Ijaz

Subjects
Medicine, Science
Abstract

Abstract The World Health Organization’s R&D Blueprint list of priority diseases for 2022 includes Lassa fever, signifying the need for research and development in emergency contexts. This disease is caused by the arenavirus Lassa virus (LASV). Being an enveloped virus, LASV should be susceptible to a variety of microbicidal actives, although empirical data to support this expectation are needed. We evaluated the virucidal efficacy of sodium hypochlorite, ethanol, a formulated dual quaternary ammonium compound, an accelerated hydrogen peroxide formulation, and a p-chloro-m-xylenol formulation, per ASTM E1052-20, against LASV engineered to express green fluorescent protein (GFP). A 10-μL volume of virus in tripartite soil (bovine serum albumin, tryptone, and mucin) was combined with 50 μL of disinfectant in suspension for 0.5, 1, 5, or 10 min at 20–25 °C. Neutralized test mixtures were quantified by GFP expression to determine log10 reduction. Remaining material was passaged on Vero cells to confirm absence of residual infectious virus. Input virus titers of 6.6–8.0 log10 per assay were completely inactivated by each disinfectant within 1–5 min contact time. The rapid and substantial inactivation of LASV suggests the utility of these microbicides for mitigating spread of infectious virus during Lassa fever outbreaks.

Published
2023
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43. Exploring the efficacy of identity priming and message framing in influencing American attitudes toward trophy hunting.

Author

Eden Rozing, Elena C Rubino, Amber Turner, and Pipiet Larasatie

Subjects
Medicine, Science
Abstract

In the United States, the general public typically disapproves of trophy hunting. Given the global ubiquity of the practice, its potential to benefit conservation when properly managed, and the substantial role played by American hunter-tourists, changing these attitudes can help to maintain the practice as a wildlife management tool. Existing trophy hunting communications, which are currently neither well-designed nor widely distributed, are unable to effectively do so. We used an online survey to explore current American attitudes regarding trophy hunting and assess the efficacy of different messaging strategies in influencing these attitudes. Respondents were randomly sorted into nine treatment groups, each of which received both an identity-focused priming item (or lack thereof, as a control) and a message about trophy hunting. The priming items prompted respondents to consider either their personal values (personal identity) or political affiliation (social identity) before reading their assigned message. All three messages evaluated in this study contained a brief informative paragraph about trophy hunting, and additional information concerning either the ecological or socioeconomic benefits associated with the practice was appended onto this paragraph for respondents assigned to treatments featuring an experimental message frame. Based on the responses of 2000 U.S. residents, we found that negative attitudes regarding trophy hunting were pervasive and resistant to change. Messages framed around the ecological or socioeconomic benefits of managed trophy hunting slightly increased approval for the practice, but identity-focused priming items had no comparable effect. Additionally, respondents' trust in the messages varied by source. This research represents a novel approach to understanding and changing public attitudes toward a highly controversial form of hunting through scientifically informed messaging. While our findings suggest several areas of improvement specifically for future trophy hunting communications, they may also be applied to outreach efforts concerning other contentious wildlife management issues.

Published
2024
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44. Integrating microbial abundance time series with fermentation dynamics of the rumen microbiome via mathematical modelling.

Author

Mohsen Davoudkhani, Francesco Rubino, Christopher J Creevey, Seppo Ahvenjärvi, Ali R Bayat, Ilma Tapio, Alejandro Belanche, and Rafael Muñoz-Tamayo

Subjects
Medicine, Science
Abstract

The rumen represents a dynamic microbial ecosystem where fermentation metabolites and microbial concentrations change over time in response to dietary changes. The integration of microbial genomic knowledge and dynamic modelling can enhance our system-level understanding of rumen ecosystem's function. However, such an integration between dynamic models and rumen microbiota data is lacking. The objective of this work was to integrate rumen microbiota time series determined by 16S rRNA gene amplicon sequencing into a dynamic modelling framework to link microbial data to the dynamics of the volatile fatty acids (VFA) production during fermentation. For that, we used the theory of state observers to develop a model that estimates the dynamics of VFA from the data of microbial functional proxies associated with the specific production of each VFA. We determined the microbial proxies using CowPi to infer the functional potential of the rumen microbiota and extrapolate their functional modules from KEGG (Kyoto Encyclopedia of Genes and Genomes). The approach was challenged using data from an in vitro RUSITEC experiment and from an in vivo experiment with four cows. The model performance was evaluated by the coefficient of variation of the root mean square error (CRMSE). For the in vitro case study, the mean CVRMSE were 9.8% for acetate, 14% for butyrate and 14.5% for propionate. For the in vivo case study, the mean CVRMSE were 16.4% for acetate, 15.8% for butyrate and 19.8% for propionate. The mean CVRMSE for the VFA molar fractions were 3.1% for acetate, 3.8% for butyrate and 8.9% for propionate. Ours results show the promising application of state observers integrated with microbiota time series data for predicting rumen microbial metabolism.

Published
2024
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45. Description of maternal and neonatal adverse events in pregnant people immunised with COVID-19 vaccines during pregnancy in the CLAP NETWORK of sentinel sites: nested case–control analysis of the immunization-associated risk – a study protocol

Author

Mercedes Colomar, Giselle Tomasso, Edgardo Abalos, Pablo Durán, Monica Chiu, Rodolfo Gomez Ponce de Leon, Diego Macías Saint-Gerons, José Luis Castro, Claudio Sosa, Alba Maria Ropero, Suzanne Jacob Serruya, Desiré Pastor, Martha Velandia-Gonzalez, Luis Mainero, Marcelo Rubino, Bremen De Mucio, and Robin Rojas-Cortés

Subjects
Medicine
Abstract

Introduction COVID-19 is associated with higher morbimortality in pregnant people compared with non-pregnant people. At present, the benefits of maternal immunisation are considered to outweigh the risks, and therefore, vaccination is recommended during pregnancy. However, additional information is needed on the safety of the vaccines in this population.Methods and analysis This a retrospective cohort nested case–control study in pregnant people who attended maternity hospitals from eight Latin American and Caribbean countries. A perinatal electronic clinical history database with neonatal and obstetric information will be used. The proportion of pregnant people immunised with COVID-19 vaccines of the following maternal and neonatal events will be described: preterm infant, small for gestational age, low birth weight, stillbirth, neonatal death, congenital malformations, maternal near miss and maternal death. Moreover, the risk of prematurity, small for gestational age and low birth weight associated with exposure to COVID-19 vaccines will be estimated. Each case will be matched with two groups of three randomly selected controls. Controls will be matched by hospital and mother’s age (±3 years) with an additional matching by delivery date and conception time in the first and second control groups, respectively. The estimated required sample size for the main analysis (exposure to any vaccine) concerning ‘non-use’ is at least 1009 cases (3027 controls) to detect an increased probability of vaccine-associated event risk of 30% and at least 650 cases (1950 controls) to detect 30% protection. Sensitivity and secondary analyses considering country, type of vaccine, exposure windows and completeness of immunisation will be reported.Ethics The study protocol was reviewed by the Ethical Review Committee on Research of the Pan American Health Organization. Patient informed consent was waived due to the retrospective design and the utilisation of anonymised data (Ref. No: PAHOERC.0546.01). Results will be disseminated in open access journals.

Published
2024
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46. Skeletal muscle overexpression of sAnk1.5 in transgenic mice does not predispose to type 2 diabetes

Author

E. Pierantozzi, L. Raucci, S. Buonocore, E. M. Rubino, Q. Ding, A. Laurino, F. Fiore, M. Soldaini, J. Chen, D. Rossi, P. Vangheluwe, H. Chen, and V. Sorrentino

Subjects
Medicine, Science
Abstract

Abstract Genome-wide association studies (GWAS) and cis-expression quantitative trait locus (cis-eQTL) analyses indicated an association of the rs508419 single nucleotide polymorphism (SNP) with type 2 diabetes (T2D). rs508419 is localized in the muscle-specific internal promoter (P2) of the ANK1 gene, which drives the expression of the sAnk1.5 isoform. Functional studies showed that the rs508419 C/C variant results in increased transcriptional activity of the P2 promoter, leading to higher levels of sAnk1.5 mRNA and protein in skeletal muscle biopsies of individuals carrying the C/C genotype. To investigate whether sAnk1.5 overexpression in skeletal muscle might predispose to T2D development, we generated transgenic mice (TgsAnk1.5/+) in which the sAnk1.5 coding sequence was selectively overexpressed in skeletal muscle tissue. TgsAnk1.5/+ mice expressed up to 50% as much sAnk1.5 protein as wild-type (WT) muscles, mirroring the difference reported between individuals with the C/C or T/T genotype at rs508419. However, fasting glucose levels, glucose tolerance, insulin levels and insulin response in TgsAnk1.5/+ mice did not differ from those of age-matched WT mice monitored over a 12-month period. Even when fed a high-fat diet, TgsAnk1.5/+ mice only presented increased caloric intake, but glucose disposal, insulin tolerance and weight gain were comparable to those of WT mice fed a similar diet. Altogether, these data indicate that sAnk1.5 overexpression in skeletal muscle does not predispose mice to T2D susceptibility.

Published
2023
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47. Translucencia nucal aumentada: capacidad predictiva para cromosomopatías.

Author

Andrea Rubino Salaverria and Freddy González Arias

Subjects
Cromosomopatías, Translucencia nucal, Líquido amniótico, Vellosidades coriales, Marcador ecográfico, Cariotipo fetal, Medicine
Abstract

Introducción: La Translucencia Nucal (TN) es un marcador ecográfico para el diagnóstico de cromosomopatías en el primer trimestre de gestación. La TN aumentada se relaciona con cromosomopatías principalmente trisomías 21 en 50% . Asociado a un mal pronóstico perinatal y neonatal, aunque existe un porcentaje de fetos normales a pesar de una TN aumentada Objetivo: Correlacionar los resultados de cariotipo en muestras de líquido amniótico (LA) de vellosidades coriales (VC) en los pacientes con TN aumentada durante el tamizaje ultrasonográfico. Método: Estudio analítico, restrospectivo, cohorte tipo III. La población se confromó por 5.969 muestras de pacientes con estudio citogenénico entre el año 2011-2022, con indicación de TN aumentada, se obtuvieron 141 resultados y la muestra se conformó por 52 LA donde se reportó el grosor de la TN y 30 resultados BVC que cumplieron con los criterios de inclusión. Resultados: Se reportó cariotipo fetal en LA normal en 46,30% y anormal en 57.70%. En VC se reporto 60% normal y 40% anormal. La trisomía 21 fue la cromosomopatía más común en ambos tipos de estudios, seguida de Monosomía X, Trisomía 18 y Trisomía 13. La Monosomía X es la que presenta la TN de mayor grosor en el grupo de LA y la Trisomia 21 en el grupo de VC. Conclusión: El aumento del grosor de la translucencia nucal fetal a las 11 a 14 semanas es una expresión fenotípica común de cromosomopatías y malformaciones, sin embargo se puede presentar aumentada en fetos euploides, con desarrollo posnatal normal.

Published
2023

48. Elevated gonadotropin levels are associated with increased biomarker risk of Alzheimer's disease in midlife women

Author

Matilde Nerattini, Federica Rubino, Steven Jett, Caroline Andy, Camila Boneu, Camila Zarate, Caroline Carlton, Susan Loeb-Zeitlin, Yelena Havryliuk, Silky Pahlajani, Schantel Williams, Valentina Berti, Paul Christos, Matthew Fink, Jonathan P. Dyke, Roberta Diaz Brinton, and Lisa Mosconi

Subjects
gonadotropin (FSH and LH), Alzheimer's disease, neuroimaging, menopause, women, positron emission tomography (PET), Medicine
Abstract

IntroductionIn preclinical studies, menopausal elevations in pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), trigger Alzheimer's disease (AD) pathology and synaptic loss in female animals. Herein, we took a translational approach to test whether gonadotropin elevations are linked to AD pathophysiology in women.MethodsWe examined 191 women ages 40–65 years, carrying risk factors for late-onset AD, including 45 premenopausal, 67 perimenopausal, and 79 postmenopausal participants with clinical, laboratory, cognitive exams, and volumetric MRI scans. Half of the cohort completed 11C-Pittsburgh Compound B (PiB) amyloid-β (Aβ) PET scans. Associations between serum FSH, LH and biomarkers were examined using voxel-based analysis, overall and stratified by menopause status. Associations with region-of-interest (ROI) hippocampal volume, plasma estradiol levels, APOE-4 status, and cognition were assessed in sensitivity analyses.ResultsFSH levels were positively associated with Aβ load in frontal cortex (multivariable adjusted P ≤ 0.05, corrected for family wise type error, FWE), an effect that was driven by the postmenopausal group (multivariable adjusted PFWE ≤ 0.044). LH levels were also associated with Aβ load in frontal cortex, which did not survive multivariable adjustment. FSH and LH were negatively associated with gray matter volume (GMV) in frontal cortex, overall and in each menopausal group (multivariable adjusted PFWE ≤ 0.040), and FSH was marginally associated with ROI hippocampal volume (multivariable adjusted P = 0.058). Associations were independent of age, clinical confounders, menopause type, hormone therapy status, history of depression, APOE-4 status, and regional effects of estradiol. There were no significant associations with cognitive scores.DiscussionIncreasing serum gonadotropin levels, especially FSH, are associated with higher Aβ load and lower GMV in some AD-vulnerable regions of midlife women at risk for AD. These findings are consistent with preclinical work and provide exploratory hormonal targets for precision medicine strategies for AD risk reduction.

Published
2023
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49. Long-term hospitalisations in survivors of paediatric solid tumours in France

Author

Daniel Bejarano-Quisoboni, Nathalie Pelletier-Fleury, Rodrigue S. Allodji, Brice Fresneau, Majorie Boussac, Hélène Pacquement, François Doz, Delphine Berchery, Claire Pluchart, Piere-Yves Bondiau, Julie Nys, Angela Jackson, Charlotte Demoor-Goldschmidt, Agnes Dumas, Cécile Thomas-Teinturier, Boris Schwartz, Neige Journy, Carole Rubino, Giao Vu-Bezin, Dominique Valteau-Couanet, Chiraz El-Fayech, Christelle Dufour, Nadia Haddy, and Florent de Vathaire

Subjects
Medicine, Science
Abstract

Abstract The late effects of treatments for childhood cancers may lead to severe and multiple health conditions requiring hospitalisation. We aimed to estimate the hospitalisation rate among childhood cancer survivors (CCS) in France, to compare them with the general population and to investigate the associated factors. We matched total of 5439 5-year solid CCS diagnosed before the age of 21 between 1945 and 2000 by sex, birth year and region of residence to 386,073 individuals of the French general population. After linkage with the national hospital discharge database, we estimated the relative hospitalisation rate (RHR), the absolute excess risks (AERs) and the relative bed-day ratio (RBDR) during 2006–2018. We used generalised linear models to estimate associations between hospitalisation and survivor characteristics. Overall, the RHR was 2.49 (95% confidence interval [CI] 2.46–2.52) and the RBDR was 3.49 (95% CI 3.46–3.51). We found that neoplasm-related hospitalisations had the highest AER (105.8 per 1000 person-years), followed by genitourinary system diseases (34.4 per 1000 person-years) and cardiovascular diseases (19.2 per 1000 person-years). In adjusted analysis, CCS treated with chemotherapy (risk ratio [RR] 1.62, 95% CI 1.53–1.70), radiotherapy (RR 2.11, 95% CI 1.99–2.24) or both (RR 2.59, 95% CI 2.46–2.73) had a higher risk of hospitalisation than the ones who had not received any of these treatments. CCS treated during the past decades by chemotherapy and/or radiotherapy now had a higher hospitalisation risk for all main categories of diagnosis than the general population. Prevention strategies and medical surveillance programmes may promote a long-term decrease in the hospitalisation rate among CSS.

Published
2022
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50. Interleukin-6 and Its Soluble Receptor Complex in Intensive Care Unit COVID-19 Patients: An Analysis of Second Wave Patients

Author

Gaetano Di Spigna, Daniela Spalletti Cernia, Bianca Covelli, Maria Vargas, Valentina Rubino, Carmine Iacovazzo, Filomena Napolitano, and Loredana Postiglione

Subjects
acute respiratory distress, COVID-19, cytokine storm, IL-6 and soluble receptor complex, comorbidity index, second wave of infection, Medicine
Abstract

In December 2019, a SARS-CoV-2 virus, coined Coronavirus Disease 2019 (COVID-19), discovered in Wuhan, China, affected the global population, causing more than a million and a half deaths. Since then, many studies have shown that the hyperinflammatory response of the most severely affected patients was primarily related to a higher concentration of the pro-inflammatory cytokine interleukin-6, which directly correlated with disease severity and high mortality. Our study analyzes IL-6 and its soluble receptor complex (sIL-6R and sgp130) in critically ill COVID-19 patients who suffered severe respiratory failure from the perspective of the second COVID wave of 2020. A chemiluminescent immunoassay was performed for the determination of IL6 in serum together with an enzyme-linked immunosorbent assay to detect serum levels of sIL-6R and sgp130, which confirmed that the second wave’s serum levels of IL-6 were significantly elevated in the more severe patients, as with the first 2019 COVID-19 wave, resulting in adverse clinical outcomes. At present, considering that no specific treatment for severe COVID-19 cases in its later stages exists, these molecules could be considered promising markers for disease progression, illness severity, and risk of mortality.

Published
2023
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