13 results on '"Ngandjio A"'
Search Results
2. Histoplasmosis in HIV-Infected Persons, Yaoundé, Cameroon
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Christine E. Mandengue, Antoinette Ngandjio, and Paul J.A. Atangana
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Cameroon ,HIV/AIDS and other retroviruses ,tuberculosis ,histoplasmosis ,fungi ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2015
- Full Text
- View/download PDF
3. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010.
- Author
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Rolf S Kaas, Antoinette Ngandjio, Ariane Nzouankeu, Achiraya Siriphap, Marie-Christine Fonkoua, Frank M Aarestrup, and Rene S Hendriksen
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Medicine ,Science - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
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- 2016
- Full Text
- View/download PDF
4. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
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Marie-Christine Fonkoua, M. Massongo, W. Mbanzouen, D. Barger, L. Ngando, Ariane Nzouankeu, J. Tchatchueng, E.W. Pefura Yone, M. C. Tejiokem, A. Ngandjio, Université de Yaoundé I, Jamot hospital, Service de bactériologie [Yaoundé, Cameroun], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire d'Epidémiologie et de Santé Publique, Bordeaux population health (BPH), and Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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0301 basic medicine ,Adult ,Male ,Veterinary medicine ,Salmonella ,Article Subject ,Adolescent ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Drug resistance ,Microbial Sensitivity Tests ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Drug Resistance, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Cameroon ,Medical prescription ,Child ,Retrospective Studies ,General Immunology and Microbiology ,biology ,Bacteria ,Pseudomonas aeruginosa ,business.industry ,Infant ,General Medicine ,Bacterial Infections ,Acinetobacter ,Middle Aged ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Anti-Bacterial Agents ,Multiple drug resistance ,Child, Preschool ,Medicine ,Female ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,business ,Research Article - Abstract
Introduction. Antimicrobial resistance represents a growing public health threat. One of the World Health Organization’s strategic objectives is “strengthening knowledge through surveillance and research.” Sub-Saharan African countries are still far from achieving this objective. We aimed to estimate and compare the prevalence of antibacterial resistance in 2010 and 2017 in Cameroon. Methods. We conducted a retrospective study on all clinical specimens cultured in Centre Pasteur du Cameroun (CPC) in 2010 and 2017. Data were extracted from the CPC’s laboratory data information system software and then managed and analyzed using R. Bacterial resistance rates were calculated in each year and compared using chi-square or Fisher’s tests, and relative changes were calculated. Outcomes included acquired resistance (AR), WHO priority resistant pathogens, some specific resistances of clinical interest, and resistance patterns (multi, extensively, and pan drug resistances) for five selected pathogens. Results. A total of 10,218 isolates were analyzed. The overall AR rate was 96.0% (95% CI: 95.4–96.6). Most of WHO priority bacterial resistance rates increased from 2010 to 2017. The most marked increases expressed as relative changes concerned imipenem-resistant Acinetobacter (6.2% vs. 21.6%, +248.4%, p = 0.02 ), imipenem-resistant Pseudomonas aeruginosa (13.5% vs. 23.5%, +74.1%, p < 0.01 ), 3rd generation-resistant Enterobacteriaceae (23.8% vs. 40.4%, +65.8%, p < 10 − 15 ), methicillin-resistant Staphylococcus aureus (27.3% vs. 46.0%, +68.6%, p < 0.002 ), fluoroquinolone-resistant Salmonella (3.9% vs. 9.5%, +142.9%, p = 0.03 ), and fluoroquinolone-resistant Enterobacteriaceae (32.6% vs. 54.0%, +65.8%, p < 10 − 15 ). For selected pathogens, global multidrug resistance was high in 2010 and 2017 (74.9% vs. 78.0% +4.1%, p = 0.01 ), intensively drug resistance rate was 5.8% (7.0% vs. 4.7%; p = 0.07 ), and no pan drug resistance has been identified. Conclusion. Bacterial resistance to antibiotics of clinical relevance in Cameroon was high and appeared to increase between 2010 and 2017. There is a need for regular surveillance of antibacterial resistance to inform public health strategies and empirically inform prescription practices.
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- 2021
5. Genomic history of the seventh pandemic of cholera in Africa
- Author
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François-Xavier Weill, Karen H. Keddy, Mireille Dosso, Sandra Moore, Anne Laure Page, Renaud Piarroux, Jean Michel Fournier, Antoinette Ngandjio, Monakhova Ev, Julian Parkhill, Sébastien Breurec, Monzer Hamze, Nizar Fawal, Henrik Salje, Fati Sidikou, Elisabeth Njamkepo, Asish K. Mukhopadhyay, Maria Damian, Gordon Dougan, Kathryn E. Holt, Marie Laure Quilici, Daryl Domman, Raymond Bercion, Jean Rauzier, Martin A. Mengel, Cheryl L. Tarr, Patrick A. D. Grimont, Benoit Garin, Laurence Bonte, Goutam Chowdhury, Roland Grunow, Jean Louis Koeck, Berthe Marie Njanpop-Lafourcade, Guillaume Sapriel, Myriam Henkens, Carlo Pazzani, Christiane Bouchier, Ankur Mutreja, Nicholas R. Thomson, Francisco J. Luquero, Thandavarayan Ramamurthy, Bactéries pathogènes entériques (BPE), Institut Pasteur [Paris] (IP), The Wellcome Trust Sanger Institute [Cambridge], Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, National Institute for Communicable Diseases [Johannesburg] (NICD), University of the Witwatersrand [Johannesburg] (WITS), Modélisation mathématique des maladies infectieuses - Mathematical modelling of Infectious Diseases, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Hôpital de la Timone [CHU - APHM] (TIMONE), Université de la Méditerranée - Aix-Marseille 2, Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Dakar, Epicentre [Paris] [Médecins Sans Frontières], Centre Pasteur du Cameroun, Institut Pasteur de Côte d'Ivoire, Rostov-on-Don Research Institute for Plague Control, Génomique (Plate-Forme) - Genomics Platform, Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), University of Cambridge [UK] (CAM), Translational Health Science and Technology Institute [Faridabad] (THSTI), Robert Koch Institute [Berlin] (RKI), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Cantacuzino Institute [Romania], Agence de Médecine Préventive, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Université Libanaise, Médecins Sans Frontières Belgique, National Institute of Cholera and Enteric Diseases, Hôpital Bouffard - Centre Hospitalier des Armées Bouffard [Djibouti], Service de Santé des Armées, Centre National de Référence des Vibrions et du Choléra - National Reference Center Vibrios and Cholera (CNR), Biodiversité des Bactéries Pathogènes Émergentes, University of Melbourne, London School of Hygiene and Tropical Medicine (LSHTM), This study was supported by the Institut Pasteur and the Institut Pasteur International Network, the Institut de Veille Sanitaire, the French government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant no. ANR-10-LABX-62-IBEID), the Fondation Le Roch-Les Mousquetaires, the Wellcome Trust through grant 098051 to the Sanger Institute, and the Indian Council of Medical Research, New Delhi, India., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of Bari Aldo Moro (UNIBA), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Weill, François-Xavier [0000-0001-9941-5799], Domman, Daryl [0000-0001-5389-9185], Njamkepo, Elisabeth [0000-0001-6791-6003], Tarr, Cheryl [0000-0001-6290-365X], Fawal, Nizar [0000-0002-9914-0315], Keddy, Karen H [0000-0001-8640-2235], Salje, Henrik [0000-0003-3626-4254], Bercion, Raymond [0000-0002-0466-0407], Luquero, Francisco J [0000-0003-0885-8418], Ngandjio, Antoinette [0000-0001-9777-3656], Dosso, Mireille [0000-0003-4744-8235], Monakhova, Elena [0000-0002-9216-7777], Bouchier, Christiane [0000-0003-3549-1543], Pazzani, Carlo [0000-0003-0414-2965], Grunow, Roland [0000-0001-8966-8469], Sidikou, Fati [0000-0002-0269-5454], Njanpop-Lafourcade, Berthe-Marie [0000-0001-6211-6402], Page, Anne-Laure [0000-0001-7952-3178], Koeck, Jean-Louis [0000-0002-6493-3905], Dougan, Gordon [0000-0003-0022-965X], Grimont, Patrick AD [0000-0002-6264-136X], Parkhill, Julian [0000-0002-7069-5958], Holt, Kathryn E [0000-0003-3949-2471], Piarroux, Renaud [0000-0002-4151-4134], Thomson, Nicholas R [0000-0002-4432-8505], and Apollo - University of Cambridge Repository
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0301 basic medicine ,MESH: Pandemics ,Asia ,Lineage (genetic) ,Genomic data ,Zoology ,MESH: Vibrio cholerae O1 ,Biology ,MESH: Genome, Bacterial ,medicine.disease_cause ,Africa, Southern ,West africa ,03 medical and health sciences ,Cholera ,MESH: Cholera ,Phylogenetics ,MESH: Africa, Western ,parasitic diseases ,Pandemic ,medicine ,Humans ,MESH: Phylogeny ,Pandemics ,Phylogeny ,MESH: Africa, Eastern ,MESH: Humans ,Multidisciplinary ,MESH: Asia ,Transmission (medicine) ,MESH: Genomics ,Vibrio cholerae O1 ,Genomics ,Africa, Eastern ,medicine.disease ,3. Good health ,Africa, Western ,030104 developmental biology ,Vibrio cholerae ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Africa, Southern ,Genome, Bacterial - Abstract
Wave upon wave of diseaseThe cholera pathogen,Vibrio cholerae, is considered to be ubiquitous in water systems, making the design of eradication measures apparently fruitless. Nevertheless, local and globalVibriopopulations remain distinct. Now, Weillet al.and Dommanet al.show that a surprising diversity between continents has been established. Latin America and Africa bear different variants of cholera toxin with different transmission dynamics and ecological niches. The data are not consistent with the establishment of long-term reservoirs of pandemic cholera or with a relationship to climate events.Science, this issue p.785, p.789
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- 2017
6. Trends in Gonococcal Antimicrobial Resistance in Cameroon: Before and After the Launching of the Gonococcal Antimicrobial Surveillance Program
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Esther Sokeng, Carole Tayimetha, Ariane Nzouankeu, Marcelle Abanda, Marie-Christine Fonkoua, Gaëlle Tchouwa, and Antoinette Ngandjio
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Antibiotic resistance ,business.industry ,Medicine ,Antimicrobial ,business ,Microbiology - Published
- 2016
7. Molecular characterization of multidrug resistant Salmonella from chicken and human in Yaounde
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Awa Aidara-Kane, Marguerite Wouafo Wouafo, Marie-Christine Fonkoua, Thomas Njiné, Ariane Nzouankeu, and Antoinette Ngandjio
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Salmonella ,Nalidixic acid ,biology ,Tetracycline ,Integron ,Antimicrobial ,medicine.disease_cause ,Virology ,Microbiology ,Multiple drug resistance ,Antibiotic resistance ,medicine ,biology.protein ,Pulsed-field gel electrophoresis ,medicine.drug - Abstract
Salmonella is one of the most common foodborne pathogens worldwide and chicken has been recognized as its main reservoir for human. The aim of this study was to investigate the occurrence of antimicrobial resistance genes in Salmonella isolates from chicken and human, and evaluate their genetic relationship. A total of 200 Salmonella strains (103 from Chicken and 97 from humans) were collected from 2006 to 2007 in Yaounde, and characterized for their antimicrobial susceptibility to a panel of 16 antimicrobials. Presence of antimicrobial resistance genes, class1 and class 2 integrons was investigated by PCR in Multidrug Resistant (MDR) isolates. Pulsed-Field Gel Electrophoresis (PFGE) analysis was used to investigate their genetic relatedness. Only serotypes Enteritidis, Hadar, Bareilly and II were recovered from both chicken and human. Overall, 16 isolates (8%) were susceptible to all antimicrobials including third generation cephalosporins and fluoroquinolons. Resistance was mostly observed to tetracycline (57%) in chicken isolates and to sulfonamides (78.3%) in human isolates. 78 (15.3%) strains were MDR. Class 1 integron was predominant in MDR Hadar and Typhimurium isolates. TEM-1 was the unique Extended Spectrum β-lactamase (ESBL) detected in the β-lactam resistant Typhimurium strain isolated from human. No qnr genes were identified in nalidixic acid resistant isolates. PFGE typing of Enteritidis isolates using XbaI restriction enzyme showed close genetic relationship between chicken and human isolates. For Hadar and Typhimurirum, variety of restriction patterns was observed. These results highlight the need for continuous surveillance of antimicrobial resistance in Salmonella isolates in Cameroon.
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- 2016
8. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
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Frank Møller Aarestrup, Antoinette Ngandjio, Rene S. Hendriksen, Achiraya Siriphap, Ariane Nzouankeu, Marie-Christine Fonkoua, and Rolf Sommer Kaas
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Bacterial Diseases ,0301 basic medicine ,Disease reservoir ,Marine and Aquatic Sciences ,lcsh:Medicine ,Plant Science ,Pathology and Laboratory Medicine ,Plant Genetics ,medicine.disease_cause ,El Tor ,Genome ,Disease Outbreaks ,Geographical Locations ,Cholera ,Medicine and Health Sciences ,Plant Genomics ,Cameroon ,lcsh:Science ,Phylogeny ,Genetics ,Multidisciplinary ,biology ,Vibrio cholerae O1 ,Genomics ,Anti-Bacterial Agents ,Bacterial Pathogens ,Infectious Diseases ,Medical Microbiology ,Vibrio cholerae ,Pathogens ,Research Article ,Neglected Tropical Diseases ,Freshwater Environments ,Biotechnology ,Genotype ,Chad ,Microbial Sensitivity Tests ,Serogroup ,History, 21st Century ,Polymorphism, Single Nucleotide ,Microbiology ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,Phylogenetics ,Microbial Control ,Vibrio Cholerae ,parasitic diseases ,medicine ,Humans ,Microbial Pathogens ,Disease Reservoirs ,Vibrio ,Pharmacology ,Bacteria ,Ecology and Environmental Sciences ,lcsh:R ,Organisms ,Biology and Life Sciences ,Aquatic Environments ,Outbreak ,Sequence Analysis, DNA ,Bodies of Water ,Tropical Diseases ,medicine.disease ,biology.organism_classification ,Lakes ,030104 developmental biology ,People and Places ,Africa ,Earth Sciences ,Multilocus sequence typing ,Plant Biotechnology ,lcsh:Q ,Antimicrobial Resistance ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
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- 2016
9. Prevalence and Antimicrobial Resistance of Salmonella Serotypes in Chickens from Retail Markets in Yaounde (Cameroon)
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Junie Atangana Kinfack, Marie-Christine Fonkoua, Guy Ejenguele, Thomas Njiné, Ariane Nzouankeu, Antoinette Ngandjio, and Marguerite Wouafo
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Microbiology (medical) ,Salmonella ,Meat ,Nalidixic acid ,Tetracycline ,Immunology ,Food Contamination ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Microbiology ,Drug Resistance, Multiple, Bacterial ,Ampicillin ,Clavulanic acid ,Drug Resistance, Bacterial ,Prevalence ,medicine ,Animals ,Cameroon ,Serotyping ,Pharmacology ,Amoxicillin ,Anti-Bacterial Agents ,Electrophoresis, Gel, Pulsed-Field ,Ciprofloxacin ,Streptomycin ,Chickens ,medicine.drug - Abstract
From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonella serotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin-tetracycline-nalidixic acid. These results highlight once more the need for surveillance of Salmonella contamination in poultry.
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- 2010
10. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
- Author
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Pardos de la Gandara, Maria, Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos De La Gándara, María, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, Weill, François-Xavier, Institut Pasteur [Paris], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), University of Melbourne, The Wellcome Trust Sanger Institute [Cambridge], Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium] (WIV-ISP), Institut Pasteur de Bangui, This study was supported by the Institut Pasteur, the Réseau International des Instituts Pasteur, the Institut de Veille Sanitaire, and the French Government Investissement d’Avenir Program (Integrative Biology of Emerging Infectious Diseases, Laboratory of Excellence, grant ANR-10-LABX-62-IBEID)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)
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Serotype ,MESH: Geography ,Epidemiology ,lcsh:Medicine ,Salmonella enterica serotype Typhi ,Drug resistance ,Salmonella typhi ,Plasmid ,Drug Resistance, Multiple, Bacterial ,MDR ,MESH: Genetic Variation ,bacteria ,0303 health sciences ,MESH: Guinea ,MESH: Microbial Sensitivity Tests ,Geography ,biology ,MESH: Polymorphism, Single Nucleotide ,MESH: Salmonella typhi ,Dispatch ,PFGE ,Anti-Bacterial Agents ,Infectious Diseases ,Salmonella enterica ,pulsed-field gel electrophoresis ,Microbiology (medical) ,Microbial Sensitivity Tests ,Polymorphism, Single Nucleotide ,Typhoid fever ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,multidrug resistance ,MESH: Anti-Bacterial Agents ,medicine ,Humans ,lcsh:RC109-216 ,MESH: Typhoid Fever ,Typhoid Fever ,030304 developmental biology ,Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa ,MESH: Humans ,030306 microbiology ,Haplotype ,lcsh:R ,Genetic Variation ,MESH: Drug Resistance, Multiple, Bacterial ,MESH: Haplotypes ,biology.organism_classification ,medicine.disease ,Virology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Multiple drug resistance ,Haplotypes ,Guinea ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
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- 2015
11. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students
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Anne Bianchi, Marie Christine Fonkoua, Antoinette Ngandjio, Bertille de Barbeyrac, Christiane Bébéar, Jocelyn Thonnon, Françoise Lunel, and M. Clerc
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Microbiology (medical) ,Genotype ,HpaII ,Restriction Mapping ,EcoRI ,Porins ,Chlamydia trachomatis ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Microbiology ,Male Urogenital Diseases ,medicine ,Humans ,Cameroon ,Typing ,Genetics ,General Medicine ,Chlamydia Infections ,Female Urogenital Diseases ,Restriction enzyme ,biology.protein ,Restriction fragment length polymorphism ,Nested polymerase chain reaction ,Polymorphism, Restriction Fragment Length - Abstract
Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n = 14; 40 %), followed by F (n = 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories.
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- 2004
12. Emergence of multi-drug resistant Salmonella enterica serotype Stanleyville infections among children in Yaounde, Cameroon
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Paul Koki Ndombo, Antoinette Ngandjio, Marie-Christine Fonkoua, Hortense Gonsu Kamga, and Patrice Tchendjou
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Serotype ,Male ,biology ,Adolescent ,business.industry ,Infant ,Salmonella enterica ,biology.organism_classification ,Virology ,Drug Resistance, Multiple ,Microbiology ,Infectious Diseases ,Anti-Infective Agents ,Pediatrics, Perinatology and Child Health ,Salmonella Infections ,Multi drug resistant ,Medicine ,Humans ,Female ,Cameroon ,Serotyping ,business - Published
- 2011
13. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis Infection and Genotyping of Isolated C. trachomatis Strains
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Françoise Lunel, Jocelyn Thonnon, Antoinette Ngandjio, Friede Njock, Marie Christine Fonkoua, Régis Pouillot, Christiane Bébéar, Bertille de Barbeyrac, M. Clerc, and Anne Bianchi
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Microbiology (medical) ,Sexually transmitted disease ,Adult ,Male ,Genotype ,Chlamydiology and Rickettsiology ,Porins ,Chlamydia trachomatis ,medicine.disease_cause ,Risk Factors ,Prevalence ,Medicine ,Humans ,Chlamydiaceae ,Cameroon ,Genotyping ,Chlamydia ,Molecular epidemiology ,biology ,business.industry ,Chlamydia Infections ,biology.organism_classification ,medicine.disease ,Virology ,Chlamydiales ,Female ,business - Abstract
The prevalence of Chlamydia trachomatis infection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women ( P = 0.026) and a previous sexually transmitted infection in men ( P = 0.023). The most frequent genotypes determined by sequencing the omp1 genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
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