Descriptor: "MESH: Regional Blood Flow" / Topic: medicine - Searchworks@Jio Institute Digital Library Search Results

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34 results on '"MESH: Regional Blood Flow"'

1. Endothelial autophagic flux hampers atherosclerotic lesion development

Author: Pierre Julia, Adel Hammoutene, Isabelle Pic, Benoit Viollet, Marouane Kheloufi, Patrice Codogno, Johanne Poisson, Konstantin Stark, Anne-Clémence Vion, Nicolas Dupont, Cécile Devue, Michele Souyri, Alain Tedgui, Julie Lafaurie-Janvore, Abdul I. Barakat, Juliette Lasselin, Pierre-Emmanuel Rautou, Johanna Busse, Xavier Loyer, Chantal M. Boulanger, Viollet, Benoit, Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Medizinische Klinik I [Munich, Germany], Klinikum der Universität [München], Laboratoire d'hydrodynamique (LadHyX), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Hémopathies Myéloïdes : Cellules Souches, Modèles Pré-Cliniques et Recherche Translationnelle (UMR 1131), Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Service d'hépatologie [Hôpital Beaujon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by INSERM, Paris Descartes University, the « Fondation pour la Recherche Medicale » (DPC20111122979), the « Agence Nationale pour la Recherche » (ANR-14-CE12-0011, ANR-14- CE35-0022, ANR-16-CE14-0015-01) and by AFEF (2014), J.P by the ‘poste d’accueil INSERM’, and A-C.V. by CODDIM Ile de France, M.K and A.H. by the ‘Ministere de la Recherche et de l’Enseignement Superieur ’, and M.K. by the « Fondation pour la Recherche Medicale » (FDT20160435690)., Université Paris Descartes - Paris 5 (UPD5)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Signalisation et physiopathologie des cellules épithéliales, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
Subjects: 0301 basic medicine, MESH: Inflammation, senescence, Apoptosis, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Autophagic Puncta, MESH: Atherosclerosis, Mice, atherosclerotic plaques, Endothelial cell apoptosis, MESH: Animals, ComputingMilieux_MISCELLANEOUS, Cellular Senescence, [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Plaque, Atherosclerotic, endothelial cells, Cell biology, Vascular endothelium, MESH: Shear Strength, MESH: Endothelium, Vascular, medicine.symptom, Shear Strength, Senescence, Inflammation, Biology, autophagic flux, shear stress, Proinflammatory cytokine, Lesion, 03 medical and health sciences, Autophagy, medicine, Animals, Humans, MESH: Autophagy, MESH: Plaque, Atherosclerotic, Molecular Biology, MESH: Mice, Cell phenotype, MESH: Humans, MESH: Apoptosis, MESH: Cellular Senescence, Cell Biology, Atherosclerosis, Disease Models, Animal, 030104 developmental biology, Regional Blood Flow, inflammation, MESH: Regional Blood Flow, Endothelium, Vascular, MESH: Disease Models, Animal
Abstract: International audience; Blood flowing in arteries generates shear forces at the surface of the vascular endothelium that control its anti-atherogenic properties. However, due to the architecture of the vascular tree, these shear forces are heterogeneous and atherosclerotic plaques develop preferentially in areas where shear is low or disturbed. Here we review our recent study showing that elevated shear forces stimulate endothelial autophagic flux and that inactivating the endothelial macroautophagy/autophagy pathway promotes a proinflammatory, prosenescent and proapoptotic cell phenotype despite the presence of atheroprotective shear forces. Specific deficiency in endothelial autophagy in a murine model of atherosclerosis stimulates the development of atherosclerotic lesions exclusively in areas of the vasculature that are normally resistant to atherosclerosis. Our findings demonstrate that adequate endothelial autophagic flux limits atherosclerotic plaque formation by preventing endothelial apoptosis, senescence and inflammation.
Published: 2017
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2. The Accuracy of Noninvasive Hemoglobin Measurement by Multiwavelength Pulse Oximetry After Cardiac Surgery

Author: Charles Christian Arvieux, Nicolas Paleiron, Maité Guillouet, Michelle Coat, Mehdi Ould-Ahmed, Emmanuel Nowak, Gildas Gueret, Ba-Vinh Nguyen, Jean Louis Vincent, Pierre Gouny, Department of Intensive Care, Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)-Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Centre d'Investigation Clinique (CIC - Brest), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Pneumologie (NP - HIA), Hôpital d'Instruction des armées, Service de chirurgie thoracique et cardio vasculaire [Brest] (CCTV - Brest), and Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)
Subjects: Male, MESH: Monitoring, Physiologic, law.invention, 0302 clinical medicine, MESH: Postoperative Period, 030202 anesthesiology, law, Medicine, Oximetry, Postoperative Period, Prospective Studies, MESH: Aged, MESH: Cardiac Surgical Procedures, MESH: Middle Aged, medicine.diagnostic_test, Pulse (signal processing), Middle Aged, Intensive care unit, 3. Good health, Cardiac surgery, MESH: Reproducibility of Results, Shock (circulatory), Anesthesia, Hemoglobinometry, Cardiology, Female, medicine.symptom, medicine.medical_specialty, Critical Care, Anemia, MESH: Oximetry, MESH: Software, 03 medical and health sciences, Intensive care, Internal medicine, Humans, MESH: Intensive Care, Cardiac Surgical Procedures, Aged, Monitoring, Physiologic, MESH: Hemoglobinometry, MESH: Humans, business.industry, Reproducibility of Results, 030208 emergency & critical care medicine, medicine.disease, MESH: Prospective Studies, MESH: Male, Pulse oximetry, Anesthesiology and Pain Medicine, Regional Blood Flow, MESH: Regional Blood Flow, Hemoglobin, business, MESH: Female, Software, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030217 neurology & neurosurgery
Abstract: International audience; BACKGROUND: In March 2008, a new multiwavelength pulse oximeter, the Radical 7 (Rad7; Masimo Corp., Irvine, CA), was developed that offers noninvasive measurement of hemoglobin concentration. Accuracy has been established in healthy adults and some surgical patients, but not in cardiac surgery intensive care patients, a group at high risk of postoperative bleeding events and anemia in whom early diagnosis could improve management. METHODS: In this prospective, observational study conducted in a cardiovascular intensive care unit, we compared hemoglobin concentrations shown by the Rad7 with arterial hemoglobin concentrations determined by an automated hematology analyzer, XE-2100 (Roche, Neuilly sur Seine, France). Two software versions of Rad7 (V 7.3.0.1 [42 points of comparison in 14 patients] and the updated V 7.3.1.1 [61 points of comparison in 27 patients]) were studied during two 1-week periods. Bias, defined as the difference between the 2 methods (Masimo SpHb-XE-2100 laboratory hemoglobin), was calculated. A negative bias indicated that the Masimo underestimated hemoglobin compared with the laboratory analyzer. Correlation between the perfusion index given by Rad7 and the hemoglobin bias was also studied. RESULTS: Correlations between Rad7 and XE-2100 were weak for both software versions (R2=0.11 for V 7.3.0.1 and R2=0.27 for V 7.3.1.1). Mean bias was -1.3 g/dL for V 7.3.0.1 and -1.7 g/dL for V 7.3.1.1, with wide 95% prediction intervals for the bias (respectively, -4.6 to 2.1 g/dL and -5.7 to 2.3 g/dL). The absolute hemoglobin bias tended to increase when the perfusion index decreased. For the V 7.3.0.1 software, the average absolute bias was 1.9 g/dL for perfusion index2 (P=0.03). For V 7.3.1.1, the mean absolute bias was 2.1 g/dL when the perfusion index was 2 (P=0.26). CONCLUSIONS: Our study demonstrates poor correlation between hemoglobin measured noninvasively by multiwavelength pulse oximetry and a laboratory hematology analyzer. The difference was greater when the pulse oximetry perfusion index was low, as may occur in shock, hypothermia, or vasoconstriction patients. The multiwavelength pulse oximetry is not sufficiently accurate for clinical use in a cardiovascular intensive care unit.
Published: 2011
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3. Cerebral autoregulation in patients with obstructive sleep apnea syndrome during wakefulness

Author: M. Tiberge, Marek Czosnyka, V. Larrue, A. Pavy-Le Traon, Eric A. Schmidt, Nathalie Nasr, Simon, Marie Francoise, Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurologie Vasculaire [Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service d'Explorations Fonctionnelles respiratoires, Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Addenbrooke's Hospital, Cambridge University NHS Trust, Neurologie et Explorations Fonctionnelles du Système Nerveux [Toulouse], Service Neurochirurgie [CHU Toulouse], Pôle Neurosciences [CHU Toulouse], and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)
Subjects: Male, Middle Cerebral Artery, MESH: Sleep Apnea, Obstructive, MESH: Cerebrum, Blood Pressure, Polysomnography, MESH: Carbon Dioxide, MESH: Regression Analysis, Homeostasis, Autoregulation, Stroke, Sleep Apnea, Obstructive, MESH: Middle Aged, medicine.diagnostic_test, Sleep apnea, MESH: Blood Flow Velocity, MESH: Blood Pressure, MESH: Cerebrovascular Circulation, Middle Aged, Neurology, Cerebral blood flow, MESH: Polysomnography, MESH: Homeostasis, Cerebrovascular Circulation, Anesthesia, Middle cerebral artery, Regression Analysis, Female, MESH: Pressure, Blood Flow Velocity, Cerebral autoregulation, medicine.artery, Pressure, medicine, Humans, Wakefulness, Cerebrum, MESH: Humans, business.industry, MESH: Middle Cerebral Artery, Carbon Dioxide, medicine.disease, MESH: Male, respiratory tract diseases, Obstructive sleep apnea, MESH: Wakefulness, Regional Blood Flow, MESH: Regional Blood Flow, Neurology (clinical), business, MESH: Female
Abstract: International audience; BACKGROUND AND PURPOSE: Obstructive sleep apnea syndrome (OSAS) is an independent risk factor for stroke. Impairment of cerebral autoregulation may play a potential role in the pre-disposition to stroke of OSAS patients. In this study, we aimed to assess dynamic cerebral autoregulation (DCA) during wakefulness in OSAS patients and a group of matched controls. METHODS: Patients and controls were examined in the morning after an overnight complete polysomnography. Mean cerebral blood flow velocity (CBFV) in the middle cerebral artery and mean arterial blood pressure (ABP) were continuously recorded using transcranial Doppler and Finapres. DCA was assessed using the Mx autoregulatory index. Mx is a moving correlation coefficient between mean CBFV and mean ABP. More positive value of Mx indicates worse autoregulation. RESULTS: Eleven OSAS patients (mean age +/- SD; 52.6 +/- 7.9) and 9 controls (mean age +/- SD; 49.1 +/- 5.3) were enrolled. The mean apnea-hypopnea index (AHI) in the OSAS group was of 22.7 +/- 11.6. No significant difference was found between the two groups as for age, body mass index, mean ABP and endtidal CO(2) pressure. Cerebral autoregulation was impaired in OSAS patients compared with controls (Mx index: 0.414 +/- 0.138 vs. 0.233 +/- 0.100; P = 0.009). The severity of autoregulation impairment correlated to the severity of the sleep respiratory disturbance measured by the AHI (P = 0.003). CONCLUSION: Cerebral autoregulation is impaired in patients with OSAS during wakefulness. Impairment of cerebral autoregulation is correlated with the severity of OSAS.
Published: 2009
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4. Circulating Vascular Cell Adhesion Molecule-1 Is Associated With Cerebral Blood Flow Dysregulation, Mobility Impairment, and Falls in Older Adults

Author: Margaret Gagnon, Thierry Dantoine, Gregory A. Wellenius, Farzaneh A. Sorond, Achille Edem Tchalla, Thomas G. Travison, Ikechukwu Iloputaife, Lewis A. Lipsitz, Handicap, Activité, Vieillissement, Autonomie, Environnement (HAVAE), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), and Université Laval [Québec] (ULaval)
Subjects: Male, Middle Cerebral Artery, Hemodynamics, 030204 cardiovascular system & hematology, MESH: Hypertension, Cohort Studies, 0302 clinical medicine, MESH: Aged, 80 and over, MESH: Risk Factors, Risk Factors, MESH: Incidence, Prospective Studies, Endothelial dysfunction, MESH: Cohort Studies, Gait, MESH: Aged, Aged, 80 and over, Vasomotor, Incidence, Age Factors, Brain, MESH: Cerebrovascular Circulation, MESH: Accidental Falls, medicine.anatomical_structure, Cerebral blood flow, Cerebrovascular Circulation, Middle cerebral artery, Hypertension, Cardiology, Female, medicine.medical_specialty, Endothelium, Vascular Cell Adhesion Molecule-1, 03 medical and health sciences, MESH: Brain, MESH: Cross-Sectional Studies, Internal medicine, medicine.artery, MESH: Mobility Limitation, Internal Medicine, medicine, Humans, Mobility Limitation, Aged, MESH: Age Factors, MESH: Humans, business.industry, MESH: Gait, MESH: Vascular Cell Adhesion Molecule-1, MESH: Biological Markers, Blood flow, MESH: Middle Cerebral Artery, medicine.disease, MESH: Male, MESH: Prospective Studies, Transcranial Doppler, Surgery, Cross-Sectional Studies, Regional Blood Flow, MESH: Regional Blood Flow, Accidental Falls, business, MESH: Female, 030217 neurology & neurosurgery, Biomarkers, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Soluble vascular cell adhesion molecule-1 (sVCAM-1) is associated with hypertension, vascular inflammation, and systemic endothelial dysfunction. We evaluated whether elevated plasma sVCAM-1 is associated with impaired cerebrovascular function and mobility impairments in elderly people. We studied the cross-sectional relationships between plasma sVCAM-1 level, gait speed, and cerebrovascular hemodynamics, and its longitudinal relationship with falls in 680 community-dwelling participants aged ≥65 years in the Maintenance of Balance, Independent Living, Intellect, and Zest in the Elderly (MOBILIZE) Boston Study. Falls were recorded prospectively for 1 year on daily calendars. sVCAM-1 was measured by ELISA assay and beat-to-beat blood flow velocity in the middle cerebral artery during rest and in response to changes in end-tidal CO 2 was measured by transcranial Doppler ultrasound. sVCAM-1 concentration was 1094±340 ng/mL in normotensives, 1195±438 ng/mL in controlled hypertensives, and 1250±445 ng/mL in uncontrolled hypertensives ( P =0.008). The mean resting blood flow velocity and cerebral vasomotor range were, respectively, 41.0±10.3 cm/s and 1.3±0.4 cm/s per millimeter of mercury. Elevated sVCAM-1 levels indicative of endothelial dysfunction were associated with reduced resting blood flow velocity ( P =0.017) and cerebral vasomotor range ( P =0.0048). Elevated sVCAM-1 levels were associated with slower gait speed (P =0.0011) and an increased odds of injurious falls (odds ratio, 2.4; 95% confidence interval, 1.4–4.2; P =0.0028). An elevated sVCAM-1 level may be a marker of cerebral blood flow dysregulation because of endothelial damage from hypertension. It may also signal the presence of cerebral microvascular disease and its clinical consequences, including slow gait speed and falls.
Published: 2015
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5. A novel device for measuring arterial stiffness using finger-toe pulse wave velocity: Validation study of the pOpmètre®

Author: Marie Briet, Erwan Bozec, Pierre Boutouyrie, Jean-Michel Halimi, Maureen Alivon, Kamel Mohammedi, Michel Marre, M. Hallab, Thao Vo-Duc Phuong, Hakim Khettab, Stéphane Laurent, Virginie Vignon, Olivier Hanon, Matthieu Plichart, Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'investigation clinique [Nancy] (CIC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Université de Lorraine (UL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), AP-HP - Hôpital Cochin Broca Hôtel Dieu [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Francois Rabelais [Tours], Université de Nantes (UN), Service d'endocrinologie, diabétologie et nutrition [CHU Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Université Paris Descartes - Paris 5 (UPD5)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM), CIC-Nancy, Institut Lorrain du Coeur et des Vaisseaux Louis Mathieu [Nancy]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris]-Université Paris Diderot - Paris 7 (UPD7)
Subjects: Male, Time Factors, MESH: Cold Temperature, MESH: Observer Variation, MESH: Hypertension, MESH: Vascular Stiffness, MESH: Aged, 80 and over, Finger-to-toe transit time, Pulse wave velocity, Aged, 80 and over, Observer Variation, Vitesse de l’onde de pouls, MESH: Aged, MESH: Pulse Wave Analysis, MESH: Middle Aged, Pulse (signal processing), Cold pressor test, Healthy subjects, Equipment Design, General Medicine, Repeatability, MESH: Blood Flow Velocity, Middle Aged, Arterial stiffness, MESH: Case-Control Studies, MESH: Predictive Value of Tests, Cold Temperature, MESH: Reproducibility of Results, MESH: Young Adult, Hypertension, cardiovascular system, Temps de transit doigt-orteil, Female, France, Cardiology and Cardiovascular Medicine, Blood Flow Velocity, circulatory and respiratory physiology, Adult, medicine.medical_specialty, Validation study, Pulse Wave Analysis, MESH: Toes, Fingers, Young Adult, Vascular Stiffness, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Predictive Value of Tests, Pulse Wave Transit Time, medicine, Humans, cardiovascular diseases, Aged, MESH: Humans, business.industry, La rigidité artérielle, MESH: Time Factors, Reproducibility of Results, MESH: Adult, Toes, medicine.disease, MESH: Male, Surgery, body regions, MESH: France, MESH: Cognition Disorders, MESH: Fingers, Regional Blood Flow, Case-Control Studies, MESH: Regional Blood Flow, Cognition Disorders, business, MESH: Female, Biomedical engineering, MESH: Equipment Design
Abstract: International audience; BACKGROUND:The finger-toe pathway could be a good alternative for assessing arterial stiffness conveniently.AIM:To evaluate the accuracy of the pOpmètre®--a new device that measures finger-toe pulse wave velocity (ft-PWV).METHODS:The pOpmètre has two photodiode sensors, positioned on the finger and the toe. Pulse waves are recorded continuously for 20 seconds, and the difference in pulse wave transit time between toe and finger (ft-TT) is calculated. The travelled distance is estimated using subject height. Study 1 compared ft-PWV with carotid-femoral PWV (cf-PWV) obtained by the reference method (SphygmoCor®) in 86 subjects (mean age 53±20 years), including 69 patients with various pathologies and 17 healthy normotensives. Study 2 compared changes in ft-PWV and cf-PWV during a cold pressor test in 10 healthy subjects. Study 3 assessed repeatability in 45 patients.RESULTS:ft-PWV correlated significantly with cf-PWV (R2=0.43; P
Published: 2015
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6. Bilateral thenar hammer syndrome

Author: Maria Antonietta D'Agostino, Sandrine Jousse-Joulin, Alain Saraux, Emmanuel Plat, Bruno Guias, Luc Bressollette, Immunologie et Pathologie (EA2216), Université de Brest (UBO)-IFR148, CHRU Brest - Service de Rhumatologie (CHU - BREST - Rhumato), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Unité d'Echo-Doppler et de Médecine Vasculaire (BREST - ED MV), Département de Médecine Interne et Pneumologie [Brest] (DMIP - Brest), Service de Rhumatologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Ambroise Paré [AP-HP], Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), and Université de Brest (UBO)-Université de Brest (UBO)
Subjects: Male, Settore MED/16 - REUMATOLOGIA, 030204 cardiovascular system & hematology, 030218 nuclear medicine & medical imaging, Pseudoaneurysm, 0302 clinical medicine, MESH: Syndrome, Ultrasonography, Doppler, Color, Ultrasonography, MESH: Treatment Outcome, medicine.diagnostic_test, Doppler, MESH: Paresthesia, Syndrome, Ultasonography, Occupational Diseases, Treatment Outcome, medicine.anatomical_structure, MESH: Hypesthesia, Radial Artery, [SDV.IMM]Life Sciences [q-bio]/Immunology, Radiology, MESH: Radial Artery, Artery, Adult, MESH: Occupational Diseases, medicine.medical_specialty, Color, Lumen (anatomy), MESH: Ultrasonography, Doppler, Color, MESH: Hand, Hypesthesia, 03 medical and health sciences, Rheumatology, medicine.artery, medicine, Humans, Paresthesia, Radial artery, Thrombus, MESH: Humans, business.industry, MESH: Raynaud Disease, Echogenicity, Raynaud Disease, MESH: Adult, Blood flow, MESH: Tunica Intima, Hand, medicine.disease, MESH: Male, Thenar hammer syndrome, Surgery, body regions, Regional Blood Flow, Angiography, MESH: Regional Blood Flow, Tunica Intima, business
Abstract: Background Thenar hammer syndrome is a very rare condition that mimics rheumatic diseases such as carpal syndrome tunnel, Raynaud's phenomenon, and hand synovitis. Objective To describe the sonographic presentation of thenar hammer syndrome in a typical patient. Methods Grey-scale sonography and colour Doppler imaging of the hands with an iU22 scanner (Philips) were performed. Results In B mode, the lesion was seen as a large, rounded, heterogeneous area combining hypoechoic and echogenic components. Pseudoaneurysm was diagnosed based on presence of a cystic saccular formation arising directly from the adjacent artery and exhibiting an irregular thick wall with turbulent blood flow in the lumen. Colour Doppler showed blood flow in part of the lumen, the rest of which was filled with a thrombus seen as echogenic tissue. Conclusion Sonography can help to diagnose thenar hammer syndrome. Angiography may be unnecessary in patients with normal colour Doppler findings.
Published: 2011
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7. Disseminated arterial calcification and enhanced myogenic response are associated with abcc6 deficiency in a mouse model of pseudoxanthoma elasticum

Author: Patrick Lacolley, Daniel Henrion, Carlos Labat, Georges Leftheriotis, Anne Pizard, Y. Mauras, L. Martin, Gilles Kauffenstein, Theo G. M. F. Gorgels, Bertrand Toutain, E. Vessières, O. Le Saux, Linda Grimaud, Y. Le Corre, Arthur A.B. Bergen, Other departments, Amsterdam Neuroscience, Human Genetics, Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Service de Gériatrie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Département de Pharmacologie-Toxicologie [CHU Angers], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Netherlands Institute for Neuroscience (NIN), Royal Netherlands Academy of Arts and Sciences (KNAW), Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA), University of Hawai‘i [Mānoa] (UHM), MUMC+: AB Onderzoekers (9), Oogheelkunde, and RS: MHeNs - R3 - Neuroscience
Subjects: Male, MESH: Osteopontin, Messenger, Core Binding Factor Alpha 1 Subunit, MESH: Elastic Tissue, Gene mutation, Inbred C57BL, MESH: Mice, Knockout, MESH: Vascular Stiffness, calcification, Mice, Osteogenesis, MESH: SOXB1 Transcription Factors, MESH: Animals, Pseudoxanthoma Elasticum, MESH: Osteogenesis, MESH: Vascular Calcification, Mice, Knockout, physiologic, Arteries, MESH: Core Binding Factor Alpha 1 Subunit, Pseudoxanthoma elasticum, MESH: Gene Expression Regulation, 3. Good health, Arterial calcification, ABC transporters, MESH: Calcium, MESH: ATP-Binding Cassette Transporters, Biological Markers, MESH: Vasoconstriction, medicine.symptom, Multidrug Resistance-Associated Proteins, Cardiology and Cardiovascular Medicine, Chondrogenesis, MESH: Chondrogenesis, medicine.medical_specialty, MESH: Cell Transdifferentiation, Knockout, Myogenic contraction, MESH: Pseudoxanthoma Elasticum, MESH: Arterial Pressure, chemistry.chemical_element, Calcium, Biology, Article, Vascular Stiffness, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, MESH: Mice, Inbred C57BL, Internal medicine, medicine, Animals, Arterial Pressure, RNA, Messenger, Vascular Calcification, Collagen Type II, MESH: Mice, MESH: Arteries, MESH: RNA, Messenger, arteriosclerosis, Animal, SOXB1 Transcription Factors, MESH: Collagen Type II, medicine.disease, Elastic Tissue, MESH: Male, Mice, Inbred C57BL, Disease Models, Animal, Endocrinology, chemistry, Gene Expression Regulation, Regional Blood Flow, Vasoconstriction, Disease Models, Cell Transdifferentiation, Arterial stiffness, MESH: Biomarkers, RNA, ATP-Binding Cassette Transporters, Osteopontin, MESH: Regional Blood Flow, MESH: Disease Models, Animal, Biomarkers, Calcification
Abstract: Objective— Pseudoxanthoma elasticum is an inherited metabolic disorder resulting from ABCC6 gene mutations. It is characterized by progressive calcification and fragmentation of elastic fibers in the skin, retina, and the arterial wall. Despite calcium accumulation in the arteries of patients with pseudoxanthoma elasticum, functional consequences remain unknown. In the present study, we investigated arterial structure and function in Abcc6 −/− mice, a model of the human disease. Approach and Results— Arterial calcium accumulation was evaluated using alizarin red stain and atomic absorption spectrometry. Expression of genes involved in osteochondrogenic differentiation was measured by polymerase chain reaction. Elastic arterial properties were evaluated by carotid echotracking. Vascular reactivity was evaluated using wire and pressure myography and remodeling using histomorphometry. Arterial calcium accumulation was 1.5- to 2-fold higher in Abcc6 −/− than in wild-type mice. Calcium accumulated locally leading to punctuate pattern. Old Abcc6 −/− arteries expressed markers of both osteogenic (Runx2, osteopontin) and chondrogenic lineage (Sox9, type II collagen). Abcc6 −/− arteries displayed slight increase in arterial stiffness and vasoconstrictor tone in vitro tended to be higher in response to phenylephrine and thromboxane A2. Pressure-induced (myogenic) tone was significantly higher in Abcc6 −/− arteries than in wild type. Arterial blood pressure was not significantly changed in Abcc6 −/− , despite higher variability. Conclusions— Scattered arterial calcium depositions are probably a result of osteochondrogenic transdifferentiation of vascular cells. Lower elasticity and increased myogenic tone without major changes in agonist-dependent contraction evidenced in aged Abcc6 −/− mice suggest a reduced control of local blood flow, which in turn may alter vascular homeostasis in the long term.
Published: 2014
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8. Effect of Chronic Severe Diabetes on Myocardial Stunning in the Dog

Author: J. Delaye, G Hadour, Michel de Lorgeril, Rémi Forrat, René Ferrera, Laurent Sebbag, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Pôle Médico-Chirurgical de Transplantation Cardiaque Adulte, Hospices Civils de Lyon (HCL)-Hôpital Louis Pradel [CHU - HCL], Hospices Civils de Lyon (HCL), Laboratoire d'Etudes du Comportement à Long Terme des matériaux de conditionnement (LCLT), Département de recherche sur les technologies pour l'enrichissement, le démantèlement et les déchets (DE2D), CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Cardioprotection, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), and Département de recherche sur les Procédés et Matériaux pour les Environnements complexes (DPME)
Subjects: Male, medicine.medical_specialty, MESH: Myocardial Contraction, Radioactive microsphere technique, Myocardial Infarction, Ischemia, MESH: Coronary Circulation, 030204 cardiovascular system & hematology, Diabetes Mellitus, Experimental, 030218 nuclear medicine & medical imaging, MESH: Dogs, 03 medical and health sciences, Coronary circulation, Dogs, 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Coronary Circulation, Internal medicine, Diabetes mellitus, MESH: Diabetes Mellitus, Experimental, medicine, Animals, MESH: Animals, Myocardial infarction, MESH: Myocardial Stunning, Molecular Biology, Myocardial Stunning, Myocardial stunning, business.industry, Heart, medicine.disease, Myocardial Contraction, MESH: Male, 3. Good health, MESH: Heart, MESH: Myocardial Infarction, medicine.anatomical_structure, Regional Blood Flow, Coronary occlusion, Cardiology, Female, MESH: Regional Blood Flow, Cardiology and Cardiovascular Medicine, business, MESH: Female, Reperfusion injury
Abstract: International audience; In this study, we examined whether chronic severe diabetes may affect ischaemic and post-ischaemic regional myocardial dysfunction in vivo in the dog. Diabetes was chemically induced in randomized animals and major metabolic alterations were observed confirming the severity and chronicity of the diabetes. After 70 days, halothane-anaesthetized dogs underwent a 20-min coronary occlusion, followed by reperfusion. During ischaemia, global left ventricle function (dP/dtmax) was more altered (P
Published: 1998
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9. Cutaneous microvascular functional assessment during exercise: a novel approach using laser speckle contrast imaging

Author: Anne Humeau-Heurtier, Antoine Bruneau, Guillaume Mahé, Pierre Abraham, Sylvain Durand, A. Le Faucheur, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Angers (UA), Laboratoire Mouvement Sport Santé (M2S), École normale supérieure - Cachan (ENS Cachan)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École normale supérieure - Cachan (ENS Cachan), Institut Formation Education Physique et Sportive Angers (IFEPSA), Université Catholique de l'Ouest (UCO), Laboratoire d'Ingéniérie des Systèmes Automatisés (LISA), Université d'Angers (UA), Motricité, interactions, performance EA 4334 / Movement - Interactions - Performance (MIP), Le Mans Université (UM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR des Sciences et Techniques des Activités Physiques et Sportives (UFR STAPS), Université de Nantes (UN)-Université de Nantes (UN), The study was supported in part by an 'INTERFACE' grant from the 'Institut National de la Santé et de la Recherche Médicale' (INSERM) and was promoted by the University Hospital of Angers., The authors thank Lydie Gascoin, Isabelle Albertini, and Yoanna Onillon for technical help and Professor Fallou Cissé for expert reviewing and useful suggestions., Laboratoire de Physiologie et d'Explorations Vasculaires, PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Motricité, interaction, performance EA 4334 (MIP), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et Techniques des Activités Physiques et Sportives (UFR STAPS), and Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Subjects: Male, Pathology, Physiology, [SDV]Life Sciences [q-bio], Clinical Biochemistry, 030204 cardiovascular system & hematology, Hyperaemia, Laser Doppler flowmetry, 0302 clinical medicine, Laser-Doppler Flowmetry, MESH: Hyperemia/physiopathology, ComputingMilieux_MISCELLANEOUS, Skin, MESH: Skin/blood supply, 0303 health sciences, Laser Doppler velocimetry, MESH: Case-Control Studies, Forearm, medicine.anatomical_structure, Cardiology, Female, medicine.symptom, Perfusion, Adult, medicine.medical_specialty, Hyperemia, Contrast imaging, Microvascular function, MESH: Exercise/physiology, 03 medical and health sciences, Speckle pattern, Physiology (medical), Internal medicine, Vascular, Image Interpretation, Computer-Assisted, medicine, Humans, MESH: Forearm/blood supply, Laser speckle contrast imaging, Exercise, 030304 developmental biology, MESH: Humans, business.industry, Microcirculation, MESH: Adult, Blood flow, MESH: Male, Mean blood pressure, Regional Blood Flow, Case-Control Studies, MESH: Laser-Doppler Flowmetry, MESH: Regional Blood Flow, business, MESH: Microcirculation, MESH: Image Interpretation, Computer-Assisted, MESH: Female
Abstract: Cardiovascular diseases are often revealed during exercise and are associated with cutaneous blood flow (CBF) dysfunction. Studies of CBF during exercise are consequently of interest. Laser speckle contrast imaging (LSCI) allows for non-contact and real-time recording of CBF at rest. We tested whether LSCI could allow the study of CBF during a cycling exercise using a specific signal treatment procedure that removes movement-induced arte- facts from the LSCI raw signal. We recorded the baseline CBF and peak post-occlusive reactive hyperaemia (PORH) from the cutaneous forearm using LSCI and the mean blood pressure before and during cycling (80 Wat 70 rpm) in nine healthy subjects. We determined the cross-correlation coef- ficient r between LSCI traces obtained before and during cycling and before and after a specifically designed signal processing technique. The results are presented as the me- dian (25th-75th centile) and expressed as the cutaneous vascular conductance (laser speckle perfusion units (LSPU) per millimetre of mercury). Cross-correlation r in- creased from 0.226±0.140 before to 0.683±0.170 after post-processing. After signal processing, the peak PORH during exercise was reduced (0.38 (0.30-0.52) LSPU/mmHg) compared with the peak PORH during the non-exercise phase (0.69 (0.63-0.74) LSPU/mmHg, p
Published: 2013
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10. A simulation tool for dynamic contrast enhanced MRI

Author: Emmanuel L. Barbier, Clément Debacker, Thomas Christen, Nicolas Pannetier, Franck Mauconduit, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Radiology [Stanford], Stanford Medicine, Stanford University-Stanford University, This work was funded by the Institut National du Cancer, the Association pour la Recherche sur le Cancer and the Ministry for Higher Education and Research of France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., and Dojat, Michel
Subjects: Anatomy and Physiology, Medical Physics, Tumor Physiology, lcsh:Medicine, Contrast Media, Vascular permeability, Blood volume, computer.software_genre, Cardiovascular, 030218 nuclear medicine & medical imaging, Diagnostic Radiology, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Voxel, Basic Cancer Research, Diffusion (business), Cardiovascular Imaging, lcsh:Science, Physics, Multidisciplinary, Magnetic Resonance Imaging, MESH: Reproducibility of Results, Oncology, MESH: Permeability, Dynamic contrast-enhanced MRI, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], Medicine, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Image Enhancement, Radiology, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Algorithms, Research Article, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, MESH: Algorithms, Thermal diffusivity, Permeability, 03 medical and health sciences, MESH: Computer Simulation, MESH: Contrast Media, Image Interpretation, Computer-Assisted, Humans, Computer Simulation, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Computerized Simulations, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, [PHYS.PHYS.PHYS-MED-PH] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], MESH: Humans, business.industry, lcsh:R, Reproducibility of Results, Blood flow, MESH: Blood Vessels, Image Enhancement, Permeability (electromagnetism), Regional Blood Flow, Computer Science, Blood Vessels, lcsh:Q, MESH: Regional Blood Flow, Nuclear medicine, business, Physiological Processes, computer, MESH: Image Interpretation, Computer-Assisted, 030217 neurology & neurosurgery, Biomedical engineering
Abstract: International audience; The quantification of bolus-tracking MRI techniques remains challenging. The acquisition usually relies on one contrast and the analysis on a simplified model of the various phenomena that arise within a voxel, leading to inaccurate perfusion estimates. To evaluate how simplifications in the interstitial model impact perfusion estimates, we propose a numerical tool to simulate the MR signal provided by a dynamic contrast enhanced (DCE) MRI experiment. Our model encompasses the intrinsic R1 and R2 relaxations, the magnetic field perturbations induced by susceptibility interfaces (vessels and cells), the diffusion of the water protons, the blood flow, the permeability of the vessel wall to the the contrast agent (CA) and the constrained diffusion of the CA within the voxel. The blood compartment is modeled as a uniform compartment. The different blocks of the simulation are validated and compared to classical models. The impact of the CA diffusivity on the permeability and blood volume estimates is evaluated. Simulations demonstrate that the CA diffusivity slightly impacts the permeability estimates (< 5% for classical blood flow and CA diffusion). The effect of long echo times is investigated. Simulations show that DCE-MRI performed with an echo time TE = 5 ms may already lead to significant underestimation of the blood volume (up to 30% lower for brain tumor permeability values). The potential and the versatility of the proposed implementation are evaluated by running the simulation with realistic vascular geometry obtained from two photons microscopy and with impermeable cells in the extravascular environment. In conclusion, the proposed simulation tool describes DCE-MRI experiments and may be used to evaluate and optimize acquisition and processing strategies.
Published: 2013
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11. Primary liver cancer is more aggressive in HIV-HCV coinfection than in HCV infection. A prospective study (ANRS CO13 Hepavih and CO12 Cirvir)

Author: TRAN Albert, Sylvie Chevret, Gerard Thiefin, Patrizia Carrieri, Olivier Seror, Pierre Bedossa, Fabien Zoulim, Philippe Mathurin, Frédéric Charlotte, Vincent Di Martino, Christos CHRISTIDIS, Service d'Hépato-gastroentérologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Jean Verdier [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Epidémiologie et Biostatistique [Bordeaux], Université Bordeaux Segalen - Bordeaux 2-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'hépatologie médicale [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'immuno-hématologie clinique (CISIH), Hôpital Sainte-Marguerite [CHU - APHM] (Hôpitaux Sud ), Service de médecine interne et centre de référence des maladies rares [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Jean Verdier, Université Bordeaux Segalen - Bordeaux 2-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], and Hôpital Sainte-Marguerite [CHU - APHM] ( Hôpitaux Sud )
Subjects: Liver Cirrhosis, Male, Pathology, MESH : Liver Neoplasms, Cirrhosis, MESH : Aged, MESH : Prospective Studies, HIV Infections, Gastroenterology, 0302 clinical medicine, MESH: Liver Neoplasms, MESH : Female, 030212 general & internal medicine, Prospective Studies, Prospective cohort study, MESH: Carcinoma, Hepatocellular, MESH: Aged, MESH: Middle Aged, medicine.diagnostic_test, Portal Vein, Liver Neoplasms, virus diseases, MESH : Regional Blood Flow, MESH: HIV Infections, MESH : Adult, Middle Aged, 3. Good health, MESH: Hepatitis C, Chronic, Liver biopsy, Hepatocellular carcinoma, Coinfection, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, 030211 gastroenterology & hepatology, Female, alpha-Fetoproteins, MESH: Liver Cirrhosis, Primary liver cancer, MESH : Carcinoma, Hepatocellular, Adult, medicine.medical_specialty, Carcinoma, Hepatocellular, MESH : Hepatitis C, Chronic, MESH : Male, 03 medical and health sciences, Internal medicine, Biopsy, MESH : HIV Infections, medicine, MESH : Liver Cirrhosis, Humans, MESH : Middle Aged, Aged, MESH: Humans, Hepatology, business.industry, MESH : Humans, MESH: Adult, MESH: Portal Vein, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Hepatitis C, Chronic, medicine.disease, digestive system diseases, MESH: Prospective Studies, MESH: Male, MESH : alpha-Fetoproteins, Hiv hcv coinfection, Regional Blood Flow, MESH: Regional Blood Flow, MESH: alpha-Fetoproteins, business, MESH: Female, MESH : Portal Vein
Abstract: International audience; OBJECTIVE: Since HAART, primary liver cancer has emerged as an increasing cause of morbidity and mortality in patients with HIV infection. Our aim was to compare characteristics and outcome of primary liver cancer according to HIV status in HCV cirrhotic patients submitted to periodic ultrasonographic surveillance. METHODS: All patients with primary liver cancer and cirrhosis were selected from two prospective cohorts (ANRS CO12 Cirvir, viral cirrhosis, n=1081; ANRS CO13 Hepavih, HIV-HCV coinfection, n=1175). Cirrhosis was diagnosed by liver biopsy in monoHCV group and biopsy and/or non-invasive tests in HIV-HCV group. Ultrasonographic surveillance was performed every 6 months. Diagnosis of primary liver cancer was established according to EASL-AASLD guidelines. RESULTS: Primary liver cancer was diagnosed in 32 patients, 16 in each group, and corresponded to hepatocellular carcinoma in all except for two cholangiocarcinomas in HIV-HCV patients. Ultrasonographic follow-up was similar (median time since last ultrasonographic without focal lesion: 237 days in HIV-HCV group (n=12) versus 208 days in HCV group, NS). At primary liver cancer diagnosis HIV-HCV patients were markedly younger (48 vs. 60 yrs, P
Published: 2012
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12. Sildenafil increases digital skin blood flow during all phases of local cooling in primary Raynaud's phenomenon

Author: Jean-Luc Cracowski, Sophie Blaise, Claire Cracowski, Marcin Hellmann, Matthieu Roustit, Hypoxie et physiopathologies cardiovasculaire et respiratoire, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Investigation Clinique [Grenoble] (CIC Grenoble), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Hôpital Michallon-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Médecine Vasculaire, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Association des Sclérodermiques de France, Groupe Français de Recherche sur la Sclérodermie, Délégation à la Recherche Clinique et à l'Innovation, Grenoble University Hospital. Marcin Hellmann received a French government scholarship delivered by the French embassy in Poland., Roustit, Matthieu, Hypoxie : Physiopathologie Respiratoire et Cardiovasculaire (HP2), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, sildenafil, MESH: Piperazines, 030204 cardiovascular system & hematology, Piperazines, chemistry.chemical_compound, 0302 clinical medicine, Pharmacology (medical), Sulfones, Skin, MESH: Aged, MESH: Middle Aged, integumentary system, Skin temperature, MESH: Sulfones, Vascular conductance, MESH: Purines, Middle Aged, MESH: Skin Temperature, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, Anesthesia, cardiovascular system, Female, medicine.symptom, MESH: Phosphodiesterase 5 Inhibitors, Adult, Sildenafil, microcirculation, Raynaud's, local cooling, Sildenafil Citrate, Article, Nitric oxide, 03 medical and health sciences, MESH: Skin, medicine, Humans, Aged, Pharmacology, MESH: Humans, Skin blood flow, business.industry, MESH: Raynaud Disease, Raynaud Disease, MESH: Adult, Phosphodiesterase 5 Inhibitors, MESH: Male, respiratory tract diseases, Primary Raynaud's phenomenon, Blood pressure, chemistry, Purines, Regional Blood Flow, MESH: Regional Blood Flow, business, Skin Temperature, MESH: Female, 030217 neurology & neurosurgery, Vasoconstriction
Abstract: International audience; Digital skin vasoconstriction on local cooling is exaggerated in primary Raynaud's phenomenon (RP) as compared with controls. A significant part of such vasoconstriction relies on the inhibition of the nitric oxide (NO) pathway. We tested the effect of the phosphodiesterase 5 (PDE5) inhibitor sildenafil, which potentiates the effect of NO, on skin blood flow. We recruited 15 patients with primary RP, performing local cooling without sildenafil (day 1), after a single oral dose of 50 mg (day 2), and after a dose of 100 mg (day 3). Skin blood flow, skin temperature, and arterial pressure were recorded, and data were expressed as cutaneous vascular conductance (CVC). Sildenafil at 100 mg, but not 50 mg, significantly lessened the cooling-induced decrease in CVC. It also increased resting CVC and skin temperature. These data suggest that 100 mg sildenafil improves digital skin perfusion during local cooling in primary RP. The benefit of sildenafil "as required" should be confirmed in a randomized, controlled trial.
Published: 2012
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13. Normal muscle oxygen consumption and fatigability in sickle cell patients despite reduced microvascular oxygenation and hemorheological abnormalities

Author: Danièle Mougenel, Nathalie Lemonne, Maryse Etienne-Julan, Yann Lamarre, Marie Laure Lalanne-Mistrih, Olivier Hue, Marie-Dominique Hardy-Dessources, Xavier Waltz, Vanessa Tarer, Philippe Connes, Benoit Tressières, Aurélien Pichon, Protéines de la membrane érythrocytaire et homologues non-érythroides, Université des Antilles et de la Guyane (UAG)-Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Adaptations au Climat Tropical, Exercice et Santé (ACTES), Université des Antilles et de la Guyane (UAG), Réponses Cellulaires et Fonctionnelles à l'Hypoxie (LRPH), Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-UFR SMBH, Unité Transversale de la Drépanocytose, CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Hôpital Ricou, Centre d'investigation clinique Antilles-Guyane (CIC - Antilles Guyane), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de la Martinique [Fort de France]-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Service d'Orthopédie et de Traumatologie, CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Université des Antilles et de la Guyane (UAG), Hôpital Ricou-CHU Pointe-à-Pitre/Abymes [Guadeloupe], Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU de la Martinique [Fort de France]-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], and ADENIS, ANTOINE
Subjects: Male, MESH: Muscles, Anatomy and Physiology, Red Cells, Vasomotion, 030204 cardiovascular system & hematology, sickle cell syndromes, Biochemistry, MESH: Linear Models, 0302 clinical medicine, Autosomal Recessive, MESH: Oxygen Consumption, Musculoskeletal System, blood rheology, 0303 health sciences, Multidisciplinary, Fourier Analysis, Chemistry, Muscles, Muscle Biochemistry, Hematology, Sickle cell anemia, MESH: Hemorheology, Forearm, medicine.anatomical_structure, MESH: Microvessels, MESH: Muscle Fatigue, Muscle Fatigue, Cardiology, Muscle, Medicine, Female, MESH: Rest, medicine.symptom, MESH: Anemia, Sickle Cell, MESH: Oxygen, Research Article, Adult, Muscle tissue, medicine.medical_specialty, Rest, Science, Anemia, Sickle Cell, MESH: Multivariate Analysis, 03 medical and health sciences, Oxygen Consumption, Internal medicine, Genetics, medicine, Humans, Biology, muscle oxygenation, 030304 developmental biology, Clinical Genetics, Sickle Cell Disease, MESH: Humans, Muscle fatigue, Human Genetics, MESH: Adult, Blood flow, Oxygenation, Hypoxia (medical), medicine.disease, MESH: Male, Surgery, Hemoglobinopathies, Oxygen, Metabolism, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Regional Blood Flow, MESH: Forearm, Hemorheology, Microvessels, Multivariate Analysis, Linear Models, muscle strength, MESH: Regional Blood Flow, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, MESH: Female, MESH: Fourier Analysis
Abstract: Background/aimAlthough it has been hypothesized that muscle metabolism and fatigability could be impaired in sickle cell patients, no study has addressed this issue.MethodsWe compared muscle metabolism and function (muscle microvascular oxygenation, microvascular blood flow, muscle oxygen consumption and muscle microvascular oxygenation variability, which reflects vasomotion activity, maximal muscle force and local muscle fatigability) and the hemorheological profile at rest between 16 healthy subjects (AA), 20 sickle cell-hemoglobin C disease (SC) patients and 16 sickle cell anemia (SS) patients.ResultsMuscle microvascular oxygenation was reduced in SS patients compared to the SC and AA groups and this reduction was not related to hemorhelogical abnormalities. No difference was observed between the three groups for oxygen consumption and vasomotion activity. Muscle microvascular blood flow was higher in SS patients compared to the AA group, and tended to be higher compared to the SC group. Multivariate analysis revealed that muscle oxygen consumption was independently associated with muscle microvascular blood flow in the two sickle cell groups (SC and SS). Finally, despite reduced muscle force in sickle cell patients, their local muscle fatigability was similar to that of the healthy subjects.ConclusionsSickle cell patients have normal resting muscle oxygen consumption and fatigability despite hemorheological alterations and, for SS patients only, reduced muscle microvascular oxygenation and increased microvascular blood flow. Two alternative mechanisms can be proposed for SS patients: 1) the increased muscle microvascular blood flow is a way to compensate for the lower muscle microvascular oxygenation to maintain muscle oxygen consumption to normal values or 2) the reduced microvascular oxygenation coupled with a normal resting muscle oxygen consumption could indicate that there is slight hypoxia within the muscle which is not sufficient to limit mitochondrial respiration but increases muscle microvascular blood flow.
Published: 2012
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14. Perfusion MR imaging and 1H spectroscopy: their role in the diagnosis of microcystic and lipomatous meningiomas

Author: K. Dillworth, Alexandre Krainik, Basile Pasquier, Irène Troprès, A. Ashraf, Sylvie Grand, Dominique Hoffmann, J.F. Le Bas, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de neuropathologie, CHU Grenoble, Neurochirurgie, CHU Grenoble-Hôpital Michallon, Unité 3T, Université Joseph Fourier - Grenoble 1 (UJF), Service d'anesthésie, ANTE-INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale, Département de neuro-radiologie, CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Serduc, Raphael, and Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble
Subjects: Magnetic Resonance Spectroscopy, MESH: Blood Volume, MESH: Cerebral Angiography, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Magnetic Resonance Angiography, MESH: Meningioma, meningioma, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, Adipocytes, Meningeal Neoplasms, Cerebral Cortex, MESH: Aged, Blood Volume, MESH: Middle Aged, Neovascularization, Pathologic, Radiological and Ultrasound Technology, MESH: Energy Metabolism, Adult case, Middle Aged, 3. Good health, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Radiology, MESH: Tomography, X-Ray Computed, Perfusion, medicine.medical_specialty, spectroscopy, [SDV.CAN]Life Sciences [q-bio]/Cancer, Sensitivity and Specificity, perfusion, Meningioma, 03 medical and health sciences, Microcystic, [SDV.CAN] Life Sciences [q-bio]/Cancer, medicine, otorhinolaryngologic diseases, Humans, Radiology, Nuclear Medicine and imaging, Lactic Acid, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], neoplasms, MESH: Adipocytes, Aged, lipomatous, MESH: Humans, business.industry, MESH: Magnetic Resonance Spectroscopy, medicine.disease, MESH: Meningeal Neoplasms, Mr imaging, Proton magnetic resonance, MESH: Sensitivity and Specificity, MESH: Cerebral Cortex, Cerebral Angiography, nervous system diseases, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, Regional Blood Flow, MESH: Regional Blood Flow, MESH: Lactic Acid, Neurology (clinical), Energy Metabolism, Tomography, X-Ray Computed, business, MESH: Neovascularization, Pathologic, MESH: Female, Magnetic Resonance Angiography, 030217 neurology & neurosurgery
Abstract: International audience; Microcystic and lipomatous meningiomas represent two of the recognised rare subtypes of meningiomas. We describe the CT and MR findings in one adult case of each subtype with emphasis on the diagnostic benefits of perfusion-weighted MR imaging (PWI) and/or proton magnetic resonance spectroscopy (MRS).
Published: 2010
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15. Theoretical Principles of Perfusion Imaging: Application to Magnetic Resonance Imaging

Author: Le Bihan D, Le Bihan, Denis, National Institutes of Health [Bethesda] (NIH), Service NEUROSPIN (NEUROSPIN), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Subjects: Gadolinium DTPA, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Computer science, Contrast Media, Gadolinium, Perfusion scanning, MESH: Signal Processing, Computer-Assisted, MESH: Magnetic Resonance Imaging, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, MESH: Microcirculation, MESH: Animals, medicine.diagnostic_test, Brain, Signal Processing, Computer-Assisted, MESH: Cerebrovascular Circulation, General Medicine, Human brain, Pentetic Acid, MESH: Pentetic Acid, Magnetic Resonance Imaging, Microspheres, 3. Good health, medicine.anatomical_structure, Positron emission tomography, Cerebrovascular Circulation, Blood oxygenation, Perfusion, Preclinical imaging, MESH: Gadolinium DTPA, MESH: Microspheres, MESH: Brain, 03 medical and health sciences, MESH: Contrast Media, Organometallic Compounds, medicine, Animals, Humans, Radiology, Nuclear Medicine and imaging, MESH: Humans, business.industry, Microcirculation, MESH: Organometallic Compounds, Magnetic resonance imaging, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, Regional Blood Flow, Temporal resolution, MESH: Regional Blood Flow, MESH: Gadolinium, Nuclear medicine, business, 030217 neurology & neurosurgery, Biomedical engineering
Abstract: International audience; Perfusion magnetic resonance imaging (MRI) offers relative safety (no ionizing radiation), high spatial/temporal resolution, multi-orientation imaging capability, and relative low cost when compared with sophisticated techniques, such as positron emission tomography (PET). Several methods have been recently proposed to image perfusion. Some techniques mimic conventional nuclear medicine principles, but use radioactively inert tracers. Other approaches directly use blood as an endogenous natural tracer. Such methods are totally noninvasive, and offer original insights, for instance by monitoring variations in blood oxygenation in human brain cortex during activation tasks. These methods are presented and compared. Emphasis is given on advantages and drawbacks, and potential clinical applications.
Published: 1992
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16. Unilateral blood flow decrease induces bilateral and symmetric responses in the immature brain

Author: Olivier Baud, Sylvain Renolleau, Philippe Bonnin, Sébastien Fau, Christiane Charriaut-Marlangue, Sonia Villapol, Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie et biothérapies des maladies inflammatoires et autoimmunes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Service de réanimation néonatale et pédiatrique [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Physiopathologie, conséquences fonctionnelles et neuroprotection des atteintes du cerveau en développement, Université Paris Diderot - Paris 7 (UPD7)-IFR2-Institut National de la Santé et de la Recherche Médicale (INSERM), Prof J Mariani Centre de la Recherche Scientifique UMR 7102 'Mairie de Paris' 'Grace de Monaco', ANR-06-BYOS-0002,AVC in silico,Modélisation multiéchelle des mécanismes physiopathologiques mis en eu dans l'accident vasculaire cérébrale ischémique(2006), Dojat, Michel, Biologie systémique (BIOSYS) - Modélisation multiéchelle des mécanismes physiopathologiques mis en eu dans l'accident vasculaire cérébrale ischémique - - AVC in silico2006 - ANR-06-BYOS-0002 - BIOSYS - VALID, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Trousseau [APHP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: Male, Hemodynamics, MESH: Animals, Newborn, Brain Ischemia, Brain ischemia, 0302 clinical medicine, MESH: Caspase 3, Laser-Doppler Flowmetry, MESH: Animals, Common carotid artery, MESH: Laser-Doppler Flowmetry, 0303 health sciences, biology, Caspase 3, Brain, MESH: Brain Ischemia, Calpain, MESH: Cerebrovascular Circulation, medicine.anatomical_structure, Cerebral blood flow, Anesthesia, Cerebrovascular Circulation, Female, medicine.medical_specialty, MESH: Hemodynamics, MESH: Rats, Central nervous system, DNA Fragmentation, Pathology and Forensic Medicine, 03 medical and health sciences, MESH: Brain, Internal medicine, medicine.artery, medicine, Animals, MESH: DNA Fragmentation, 030304 developmental biology, medicine.disease, MESH: Male, Rats, Endocrinology, Animals, Newborn, Regional Blood Flow, Carotid artery occlusion, biology.protein, MESH: Regional Blood Flow, NeuN, MESH: Female, 030217 neurology & neurosurgery, Regular Articles
Abstract: International audience; The effects of hemodynamic changes in the developing brain have yet to be fully understood. The aim of this study was to explore the relationship between perturbations of the cerebral blood flow in the developing brain via unilateral hypoperfusion in P7 rats. As expected, nuclear caspase-3 (casp3) cleavage and DNA fragmentation were detected at 48 hours after stroke in the injured cortex. Surprisingly, casp3 was also cleaved in the contralateral cortex, although without cell death markers. Delayed (48 hours) casp3 cleavage without DNA fragmentation was also identified after unilateral common carotid artery occlusion, both in the hypoperfused cortex and the unaffected cortex, producing mirror images. Upstream calpain activation, caspase-2 cleavage, and mitochondrial cytochrome c release initiated casp3 cleavage, but did not produce preconditioning. The neuronal marker NeuN co-localized with cleaved casp3 in cortical layers II-III and VI and with gaba-amino butyric acid in layer III. Indeed, collateral supply was provided from the opposite side during carotid artery occlusion but not after reperfusion, and the number of cleaved casp3-positive cells significantly negatively correlated with the common carotid artery immediate reperfusion percentage. In summary, unilateral hypoperfusion, while insufficient to induce cell death, may active bilateral and symmetric casp3 in the P7 rat brain. Additionally, the opposite healthy hemisphere is altered due to the injury and thus should not be used as an internal control.
Published: 2009
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17. Alteration of endothelium-mediated vasodilator response in the rat hindlimb vasculature consecutive to chronic hypoxic stress: NO and EDHF involvement

Author: Cyril Reboul, Michel Dauzat, Stéphane Tanguy, Philippe Obert, A. Gibault, Physiopathologie des adaptations cardiovasculaires à l'Exercice, Avignon Université (AU), Caractéristiques féminines des dysfonctions des interfaces cardio-vasculaires (EA 2992), and Université Montpellier 1 (UM1)-Université de Montpellier (UM)
Subjects: Male, Time Factors, Physiology, MESH: Random Allocation, Blood Pressure, Vasodilation, Hindlimb, 030204 cardiovascular system & hematology, Biological Factors, Random Allocation, chemistry.chemical_compound, 0302 clinical medicine, MESH: Animals, Infusions, Intravenous, MESH: Stress, Physiological, MESH: Hindlimb, MESH: Arginine, MESH: Blood Pressure, medicine.anatomical_structure, Anesthesia, cardiovascular system, Molecular Medicine, MESH: Nitric Oxide Synthase, MESH: Endothelium, Vascular, Acetylcholine, MESH: Oxygen, medicine.drug, Atmosphere Exposure Chambers, medicine.medical_specialty, Endothelium, MESH: Rats, MESH: Atmosphere Exposure Chambers, Hypoxic stress, Arginine, Nitric Oxide, Apamin, Nitric oxide, MESH: Vasodilation, 03 medical and health sciences, MESH: Diet, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Stress, Physiological, EDHF, Internal medicine, medicine, Animals, MESH: Infusions, Intravenous, Pharmacology, business.industry, MESH: Acetylcholine, MESH: Time Factors, NO pathway, Blood flow, Vascular function, MESH: Male, Diet, Rats, Oxygen, Endocrinology, Blood pressure, chemistry, Regional Blood Flow, MESH: Nitric Oxide, MESH: Biological Factors, MESH: Regional Blood Flow, Endothelium, Vascular, Nitric Oxide Synthase, business, 030217 neurology & neurosurgery
Abstract: International audience; The previously documented impairment of hindlimb blood flow consecutive to chronic hypoxia might be related to endothelial vasomotor dysfunction. The aim of this study was to assess in-vivo the effect of chronic hypoxic stress on endothelium-mediated vasodilator response of hindlimb vascular bed, especially as regards to endothelium-derived hyperpolarizing factor (EDHF) and nitric oxide (NO) pathway contribution. Dark Agouti rats were randomly assigned to live at barometric pressure approximately 760 mmHg (N rats) or approximately 550 mmHg (CH rats). Under anesthesia, catheters were placed in the carotid artery for arterial pressure measurement, and in the saphenous vein and iliac artery for drug delivery. Hindlimb blood flow (HBF) was measured by transit-time ultrasound flowmetry, at baseline and during endothelium-dependent vasodilator response induced by intra-arterial injection of acetylcholine (0.75 ng and 7.5 ng) with and without specific blockers of NOS (L-NAME) and EDHF (Charybdotoxin+Apamin). HBF and hindlimb vascular conductance changes in response to ACh infusion were significantly lower in CH than in N rats. The mechanisms responsible for this blunted response involved impairment in both NO pathway and EDHF. The chronic hypoxia-induced alteration of NO pathway was mainly related to the bioavailability of its substrate l-Arginine, since the infusion of l-Arginine restored the endothelial response to ACh in CH rats to the level of N rats. These results demonstrate that the impairment in endothelium-mediated vasodilator response of the hindlimb vascular tree induced by chronic hypoxic stress involves both NO and EDHF.
Published: 2009
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18. Correlation of biomarkers of endothelium dysfunction and matrix remodeling in patients with systemic sclerosis

Author: Candice Trocme, Sophie Blaise, Jean-Luc Cracowski, Ghainsom D. Kom, Patrick H. Carpentier, Matthieu Roustit, Renke Maas, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), GREPI, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire de Pharmacologie (HP2), CHU Grenoble-Hôpital Michallon, Laboratoire d'Hématologie, CHU Grenoble, Hypoxie : Physiopathologie Respiratoire et Cardiovasculaire (HP2), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-IMAG-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Groupe de Recherche et d'Etudes du Processus Inflammatoire (GREPI), Université Joseph Fourier - Grenoble 1 (UJF)-Université Joseph Fourier - Grenoble 1 (UJF), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)
Subjects: Male, Pathology, Systemic disease, Gastroenterology, Scleroderma, MESH: Scleroderma, Systemic, chemistry.chemical_compound, 0302 clinical medicine, Fibrosis, Immunopathology, Immunology and Allergy, Medicine, Enzyme Inhibitors, Endothelial dysfunction, Skin, 0303 health sciences, MESH: Middle Aged, MESH: Arginine, Middle Aged, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Connective tissue disease, Extracellular Matrix, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 3. Good health, medicine.anatomical_structure, MESH: Enzyme Inhibitors, Female, MESH: Nitric Oxide Synthase, MESH: Endothelium, Vascular, medicine.medical_specialty, Endothelium, Immunology, MESH: Extracellular Matrix, Arginine, 03 medical and health sciences, Rheumatology, MESH: Skin, Internal medicine, Humans, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], 030304 developmental biology, 030203 arthritis & rheumatology, Scleroderma, Systemic, Tissue Inhibitor of Metalloproteinase-1, MESH: Humans, business.industry, MESH: Raynaud Disease, MESH: Biological Markers, Raynaud Disease, medicine.disease, MESH: Male, chemistry, MESH: Tissue Inhibitor of Metalloproteinase-1, Regional Blood Flow, MESH: Regional Blood Flow, Endothelium, Vascular, Nitric Oxide Synthase, business, Asymmetric dimethylarginine, MESH: Female, Biomarkers
Abstract: Objective.Systemic sclerosis (SSc) is a multisystem disease characterized by microvascular dysfunction and excessive fibrosis. However, the relationship between these 2 features remains unclear. Endothelial dysfunction can be assessed by quantifying plasma asymmetric dimethylarginine (ADMA), an endogenous inhibitor of endothelial nitric oxide synthase. Matrix remodeling can be assessed by quantifying serum tissue inhibitor of matrix metalloproteinases-1 (TIMP-1). Both biomarkers are elevated in patients with SSc. Our objective was to test whether plasma ADMA is correlated with serum TIMP-1.Methods.We enrolled 91 subjects, 39 patients with SSc, 28 patients with primary Raynaud’s phenomenon (RP), and 24 healthy volunteers. Plasma ADMA concentrations were measured by liquid chromatography-tandem mass spectrometry. Serum TIMP-1 concentrations were determined by ELISA.Results.Mean ADMA concentrations were higher in patients with SSc (0.68 μM ± 0.12) than in patients with primary RP or healthy volunteers (respectively, 0.56 μM ± 0.14 and 0.62 μM ± 0.12; p = 0.002). Median serum TIMP-1 concentrations were increased in patients with SSc compared to primary RP and healthy volunteers [12 (9–15), 11 (8–13), and 10 (7–13) nM, respectively; p = 0.05]. In the SSc group, we observed a statistically significant correlation between plasma ADMA and serum TIMP-1 (r = 0.34, p = 0.035).Conclusion.These data are consistent with our hypothesis of an association of endothelial dysfunction and matrix remodeling in scleroderma spectrum disorders.
Published: 2009
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19. Prevention of skin flap necrosis by estradiol involves reperfusion of a protected vascular network

Author: Jean-François Arnal, Pierre Gourdy, Céline E. Toutain, Hortense Berges, Patricia Vicendo, Julie Favre, Andrée Krust, Anne-Marie Schmitt, Marie-José Fouque, Isabelle Raymond-Letron, Laurent Brouchet, Pierre Chambon, Françoise Lenfant, Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Département d'Anatomie Pathologique, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Interactions moléculaires et réactivité chimique et photochimique (IMRCP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Centre de Recherche sur la Peau [Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Simon, Marie Francoise, Université de Toulouse (UT)-Université de Toulouse (UT)- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts (FERMAT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Time Factors, Necrosis, Physiology, Dermatologic Surgical Procedures, MESH: Mice, Knockout, Surgical Flaps, MESH: Drug Implants, MESH: Dose-Response Relationship, Drug, Brain ischemia, Mice, chemistry.chemical_compound, 0302 clinical medicine, Ischemia, MESH: Animals, MESH: Bone Marrow Transplantation, MESH: Estrogen Receptor alpha, Bone Marrow Transplantation, Skin, Drug Implants, Mice, Knockout, 0303 health sciences, Estradiol, integumentary system, MESH: Selective Estrogen Receptor Modulators, Vascular endothelial growth factor, MESH: Cell Survival, 030220 oncology & carcinogenesis, Ovariectomized rat, Female, MESH: Estradiol, medicine.symptom, Cardiology and Cardiovascular Medicine, Selective Estrogen Receptor Modulators, medicine.medical_specialty, Cell Survival, medicine.drug_class, Ovariectomy, Mice, Nude, MESH: Ovariectomy, MESH: Mice, Hairless, 03 medical and health sciences, MESH: Skin, In vivo, MESH: Mice, Inbred C57BL, Internal medicine, medicine, MESH: Mice, Nude, Animals, MESH: Mice, 030304 developmental biology, MESH: Necrosis, Mice, Hairless, Dose-Response Relationship, Drug, business.industry, MESH: Time Factors, Estrogen Receptor alpha, MESH: Surgical Flaps, medicine.disease, Mice, Inbred C57BL, Disease Models, Animal, Tamoxifen, Endocrinology, chemistry, Regional Blood Flow, Estrogen, MESH: Regional Blood Flow, MESH: Tamoxifen, MESH: Disease Models, Animal, MESH: Ischemia, business, MESH: Female, Ex vivo
Abstract: Although 17β-estradiol (E2) is protective in experimental models of myocardial and brain ischemia, its effect on skin ischemia remains unknown. Here, we assessed the protective effect of E2 in a mouse model of skin ischemia, mimicking the surgery of skin flaps. Whereas necrosis appeared in the half portion of the skin flap within 1 week after surgery in ovariectomized mice, it was reduced up to 10-fold when mice were pretreated with E2, at least 3 days before the surgery. The beneficial effect of E2 appeared to be attributable to an increase in skin survival, revealed by measuring viability of ex vivo explants and enhancement of the antiapoptotic Bcl-2 protein expression in vivo. This protective effect on the skin contributed to the protection of the vascular network and facilitated reperfusion, which was found to be accelerated in ovariectomized E2-treated mice, whereas hemorrhages were observed in untreated mice. E2 also increased expression of fibroblast growth factor-2 isoforms in the skin and circulating vascular endothelial growth factor in the serum. Finally, this protective effect of E2 was abolished in estrogen receptor–deficient mice (ERα −/− ) but maintained in chimeric mice reconstituted with ERα-deficient bone marrow, indicating dispensable action of E2 in bone marrow–derived cells. This protective effect of E2 was mimicked by treatment with tamoxifen, a selective estrogen receptor modulator. In conclusion, we have demonstrated for the first time that E2 exerts a major preventive effect of skin flap necrosis through a prevention of ischemic-induced skin lesions, including those of the vascular network, which contributes to accelerate the reperfusion of the skin flap.
Published: 2009
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20. Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen

Author: Sophie Kernéis, Mickael Lesurtel, Jean-Marc Tréluyer, Peter H. David, Valentine Brousse, Pierre Buffet, Déborah Hirt, Anne Couvelard, Guillaume Deplaine, Huot Khun, Geneviève Milon, Nicolas Goasguen, Catherine Ottone, Odile Mercereau-Puijalon, Alain Sauvanet, Sébastien Mulé, Jean-Michel Correas, Thierry Jo Molina, Innocent Safeukui, Inès Vigan-Womas, Immunologie moléculaire des parasites, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Service de Radiologie et imagerie médicale [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Saint-Vincent de Paul, Hôpital Beaujon [AP-HP], Institut Pasteur [Paris] (IP), Hôpital Hôtel-Dieu [Paris], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), This work was supported by the Institut Pasteur Transverse Research Programs (Paris, France, PTR no. 85), by the 'Fonds dédié Combattre les maladies parasitaires,' Sanofi Aventis-Ministère de L'Enseignement Supérieur et de la Recherche Institut Pasteur, the latter also supporting a postdoctoral fellowship (I.S.), and by a grant from Fondation pour la Recherche Medicale (Paris, France, V.B.). Research of the IMP Unit at Institut Pasteur is partly supported by the BioMalPar European Network of Excellence supported by a European grant (LSHP-CT-2004-503578) from the Priority 1 'Life Sciences, Genomics and Biotechnology for Health' in the 6th Framework Program (Paris, France). Research in the Immunophysiology and Intracellular Parasitism unit is partly supported by Agence Nationale de Recherche sur le SIDA (Paris, France)., and European Project: 503578,FP6-LIFESCIHEALTH,BIOMALPAR(2004)
Subjects: Erythrocytes, MESH: Spleen, Immunology, Population, Plasmodium falciparum, Spleen, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, In Vitro Techniques, Biochemistry, Microcirculation, Pathogenesis, Andrology, MESH: Microcirculation, medicine, Animals, Humans, MESH: Animals, education, MESH: Plasmodium falciparum, MESH: In Vitro Techniques, education.field_of_study, MESH: Humans, MESH: Erythrocytes, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, Hematology, MESH: Blood Flow Velocity, biology.organism_classification, Perfusion, medicine.anatomical_structure, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Cerebral Malaria, Regional Blood Flow, Circulatory system, Red pulp, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Regional Blood Flow, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, MESH: Perfusion, Blood Flow Velocity
Abstract: The current paradigm in Plasmodium falciparum malaria pathogenesis states that young, ring-infected erythrocytes (rings) circulate in peripheral blood and that mature stages are sequestered in the vasculature, avoiding clearance by the spleen. Through ex vivo perfusion of human spleens, we examined the interaction of this unique blood-filtering organ with P falciparum–infected erythrocytes. As predicted, mature stages were retained. However, more than 50% of rings were also retained and accumulated upstream from endothelial sinus wall slits of the open, slow red pulp microcirculation. Ten percent of rings were retained at each spleen passage, a rate matching the proportion of blood flowing through the slow circulatory compartment established in parallel using spleen contrast-enhanced ultrasonography in healthy volunteers. Rings displayed a mildly but significantly reduced elongation index, consistent with a retention process, due to their altered mechanical properties. This raises the new paradigm of a heterogeneous ring population, the less deformable subset being retained in the spleen, thereby reducing the parasite biomass that will sequester in vital organs, influencing the risk of severe complications, such as cerebral malaria or severe anemia. Cryptic ring retention uncovers a new role for the spleen in the control of parasite density, opening novel intervention opportunities.
Published: 2008
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21. Atrial natriuretic peptide contribution to lipid mobilization and utilization during head-down bed rest in humans

Author: Fabien Pillard, Cedric Moro, Max Lafontan, I. Harant, François Crampes, Claire Thalamas, Marie-Adeline Marques, Isabelle de Glisezinski, Michel Berlan, Simon, Marie Francoise, Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service Pharmacologie Clinique [CHU Toulouse], Pôle Santé publique et médecine publique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), CIC - Biotherapie - Toulouse, and Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: MESH: Oxidation-Reduction, MESH: Dietary Carbohydrates, Blood Glucose, Glycerol, Male, Sympathetic nervous system, MESH: Atrial Natriuretic Factor, Physiology, medicine.medical_treatment, Microdialysis, Fatty Acids, Nonesterified, Bed rest, Head-Down Tilt, Norepinephrine, Atrial natriuretic peptide, Heart Rate, MESH: Cyclic GMP, Insulin, MESH: Microdialysis, MESH: Heart Rate, Cyclic GMP, MESH: Fatty Acids, Nonesterified, Chemistry, MESH: Posture, medicine.anatomical_structure, MESH: Dietary Fats, MESH: Rest, Oxidation-Reduction, Atrial Natriuretic Factor, medicine.drug, Adult, medicine.medical_specialty, Lipolysis, Rest, Posture, Subcutaneous Fat, MESH: Insulin, Norepinephrine (medication), MESH: Subcutaneous Fat, MESH: Head-Down Tilt, MESH: Glycerol, Lipid oxidation, MESH: Norepinephrine, Physiology (medical), Internal medicine, medicine, Dietary Carbohydrates, Humans, MESH: Lipolysis, Pancreatic hormone, MESH: Humans, Pulmonary Gas Exchange, MESH: Adult, Dietary Fats, MESH: Male, MESH: Extracellular Space, Endocrinology, Regional Blood Flow, MESH: Blood Glucose, Catecholamine, MESH: Regional Blood Flow, MESH: Pulmonary Gas Exchange, Extracellular Space
Abstract: International audience; Head-down bed rest (HDBR) increases plasma levels of atrial natriuretic peptide (ANP) and decreases norepinephrine levels. We previously demonstrated that ANP promotes lipid mobilization and utilization, an effect independent of sympathetic nervous system activation, when infused into lean healthy men at pharmacological doses. The purpose of the present study was to demonstrate that a physiological increase in ANP contributes to lipid mobilization and oxidation in healthy young men. Eight men were positioned for 4 h in a sitting (control) or in a HDBR position. Indexes of lipid mobilization and hormonal changes were measured in plasma. Extracellular glycerol, an index of lipolysis, was determined in subcutaneous adipose tissue (SCAT) with a microdialysis technique. A twofold increase in plasma ANP concentration was observed after 60 min of HDBR, and a plateau was maintained thereafter. Plasma norepinephrine decreased by 30-40% during HDBR, while plasma insulin and glucose levels did not change. The level of plasma nonesterified fatty acids was higher during HDBR. SCAT lipolysis, as reflected by interstitial glycerol, as well as interstitial cGMP, the second messenger of the ANP pathway, increased during HDBR. This was associated with an increase in blood flow observed throughout HDBR. Significant changes in respiratory exchange ratio and percent use of lipid and carbohydrate were seen only after 3 h of HDBR. Thus the proportion of lipid oxidized increased by 40% after 3 h of HDBR. The rise in plasma ANP during HDBR was associated with increased lipolysis in SCAT and whole body lipid oxidation. In this physiological setting, independent of increasing catecholamines, our study suggests that ANP contributes to lipid mobilization and oxidation in healthy young men.
Published: 2007
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22. Paradoxical role of angiotensin II type 2 receptors in resistance arteries of old rats

Author: Ramaroson Andriantsitohaina, Odile Dumont, Kevin Retailleau, Frédéric Pinaud, Daniel Henrion, Laurent Loufrani, Christophe Baufreton, Arnaud Bocquet, Service de chirurgie cardio-vasculaire et thoracique, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Préconditionnement et remodelage du myocarde, Université d'Angers (UA)-UPRES EA 3860, Biologie Neurovasculaire Intégrée (BNVI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and INSERM, CNRS, Université d'Angers, Fondation pour la Recherche Médicale (FRM), Fédération Française de Cardiologie (Frederic Pinaud). Conseil Général du Maine et Loire (Arnaud Bocquet), Région des Pays de la Loire (Odile Dumont), contrat d'interface INSERM-CHU d'Angers (D. Henrion).
Subjects: Male, Angiotensin receptor, Aging, Contraction (grammar), MESH: Mesenteric Arteries, Vasodilator Agents, MESH: Vascular Resistance, Stimulation, Vasodilation, Biochemistry, Muscle, Smooth, Vascular, oxidative stress, MESH: Aging, MESH: Animals, MESH: Endothelial Cells, Tissue Distribution, Mesenteric arteries, MESH: Reactive Oxygen Species, MESH: Myocytes, Smooth Muscle, MESH: Muscle, Smooth, Vascular, Hydralazine, Mesenteric Arteries, medicine.anatomical_structure, MESH: Receptor, Angiotensin, Type 2, MESH: Vasoconstriction, medicine.symptom, MESH: Nitric Oxide Synthase Type III, Biotechnology, medicine.drug, medicine.medical_specialty, endothelium, MESH: Rats, Endothelium, Nitric Oxide Synthase Type III, Myocytes, Smooth Muscle, microcirculation, In Vitro Techniques, Nitric Oxide, Receptor, Angiotensin, Type 2, Article, MESH: Vasodilation, angiotensin II receptors, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, Genetics, Internal Medicine, medicine, Animals, MESH: Tissue Distribution, Rats, Wistar, Molecular Biology, Angiotensin II receptor type 1, MESH: Hydralazine, business.industry, Endothelial Cells, MESH: Rats, Wistar, Angiotensin II, MESH: Male, MESH: Vasodilator Agents, Rats, Endocrinology, Regional Blood Flow, Vasoconstriction, MESH: Nitric Oxide, Vascular resistance, MESH: Regional Blood Flow, Vascular Resistance, business, Reactive Oxygen Species
Abstract: International audience; The role of angiotensin II type 2 receptors (AT2Rs) remains a matter of controversy. Its vasodilatory and antitrophic properties are well accepted. Nevertheless, in hypertensive rats, AT2R stimulation induces a vasoconstriction counteracting flow-mediated dilation (FMD). This contraction is reversed by hydralazine. Because FMD is also decreased in aging, another risk factor for cardiovascular diseases, we hypothesized that AT2R function might be altered in old-rat resistance arteries. Mesenteric resistance arteries (250 mum in diameter) were isolated from old (24 months) and control (4 months) rats receiving hydralazine (16 mg/kg per day; 2 weeks) or water. FMD, NO-mediated dilation, and endothelial NO synthase expression were lower in old versus control rats. AT2R blockade improved FMD in old rats, suggesting that AT2R stimulation produced vasoconstriction. AT2R expression was higher in old rats and mainly located in the smooth muscle layer. In old rats, AT2R stimulation induced endothelium-independent contraction, which was suppressed by the antioxidant Tempol. Reactive oxygen species level was higher in old-rat arteries than in controls. Hydralazine improved FMD and NO-dependent dilation in old rats without change in AT2R expression and location. In old rats treated with hydralazine, reactive oxygen species level was reduced in endothelial and smooth muscle cells, and AT2R-dependent contraction was abolished. Thus, AT2R stimulation induced vasoconstriction through activation of reactive oxygen species production, contributing to decrease FMD in old-rat resistance arteries. Hydralazine suppressed AT2R-dependent reactive oxygen species production and AT2R-dependent contraction, improving FMD. Importantly, endothelial alterations in aging were reversible. These findings are important to consider in the choice of vasoactive drugs in aging.
Published: 2007
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23. Cerebral microcirculation shear stress levels determine Neisseria meningitidis attachment sites along the blood–brain barrier

Author: Jean-Christophe Olivo-Marin, Christine Bernard, Emmanuel Donnadieu, Elisabeth Pinard, Auguste Genovesio, Francis Jaubert, Emilie Mairey, J. Seylaz, Xavier Nassif, Guillaume Duménil, Université Paris Cité - UFR Médecine [Santé] (UPCité UFR Médecine), Université Paris Cité (UPCité), Institut National de la Santé et de la Recherche Médicale (INSERM), Analyse d'Images Quantitative (AIQ), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Recherche Cardiovasculaire de Lariboisiere, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), The work was funded by INSERM, CNRS, and Université René Descartes. E. Mairey was funded by the French Ministry of research and higher education. A. Genovesio was founded by the ANRS (Agence Nationale de Recherches sur le SIDA) and the Pasteur Institute., The authors would like to thank V. Pelicic for providing numerous transposon insertion mutants, discussions, and for reviewing the manuscript. The authors also express their gratitude to P.O. Couraud for providing the RBE4 and hCMEC/D3 cell lines. The authors wish to thank R. Isberg for pointing out the importance of shear forces in bacterial adhesion. The authors thank P. Martin and A. Maria Lennon-Dumenil for reviewing the manuscript. The authors are also grateful to P. Bongrand for helpful discussions concerning the biophysical aspects of study., Genovesio, Auguste, Université de Paris - UFR Médecine Paris Centre [Santé] (UP Médecine Paris Centre), Université de Paris (UP), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and UFR Médecine [Santé] - Université Paris Cité (UFR Médecine UPCité)
Subjects: MESH: Neisseria meningitidis/physiology, [SDV]Life Sciences [q-bio], MESH: Blood-Brain Barrier/pathology, Neisseria meningitidis, MESH: Attachment Sites, Microbiological/physiology, medicine.disease_cause, Bacterial Adhesion, 0302 clinical medicine, MESH: Microcirculation, Immunology and Allergy, Cerebral microcirculation, MESH: Animals, Cells, Cultured, 0303 health sciences, MESH: Stress, Mechanical, Articles, Adhesion, Environment, Controlled, Shock, Septic, MESH: Infant, 3. Good health, MESH: Blood-Brain Barrier/microbiology, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Blood-Brain Barrier, MESH: Meningitis, Meningococcal/pathology, Cerebrovascular Circulation, Attachment Sites, Microbiological, MESH: Shock, Septic/pathology, MESH: Blood-Brain Barrier/cytology, MESH: Cerebrovascular Circulation, MESH: Endothelial Cells/cytology, Meningitis, MESH: Cells, Cultured, MESH: Fimbriae, Bacterial/metabolism, MESH: Rats, Immunology, Meningitis, Meningococcal, Biology, Blood–brain barrier, Article, Microcirculation, Microbiology, MESH: Neisseria meningitidis/cytology, MESH: Environment, Controlled, 03 medical and health sciences, medicine, Shear stress, Animals, Humans, MESH: Bacterial Adhesion, 030304 developmental biology, MESH: Humans, 030306 microbiology, Endothelial Cells, Infant, Cell Biology, Blood flow, MESH: Capillaries/microbiology, medicine.disease, Capillaries, Rats, Regional Blood Flow, Fimbriae, Bacterial, MESH: Regional Blood Flow, Stress, Mechanical, 030215 immunology
Abstract: International audience; Neisseria meningitidis is a commensal bacterium of the human nasopharynx. Occasionally, this bacterium reaches the bloodstream and causes meningitis after crossing the blood-brain barrier by an unknown mechanism. An immunohistological study of a meningococcal sepsis case revealed that neisserial adhesion was restricted to capillaries located in low blood flow regions in the infected organs. This study led to the hypothesis that drag forces encountered by the meningococcus in the bloodstream determine its attachment site in vessels. We therefore investigated the ability of N. meningitidis to bind to endothelial cells in the presence of liquid flow mimicking the bloodstream with a laminar flow chamber. Strikingly, average blood flows reported for various organs strongly inhibited initial adhesion. As cerebral microcirculation is known to be highly heterogeneous, cerebral blood velocity was investigated at the level of individual vessels using intravital imaging of rat brain. In agreement with the histological study, shear stress levels compatible with meningococcal adhesion were only observed in capillaries, which exhibited transient reductions in flow. The flow chamber assay revealed that, after initial attachment, bacteria resisted high blood velocities and even multiplied, forming microcolonies resembling those observed in the septicemia case. These results argue that the combined mechanical properties of neisserial adhesion and blood microcirculation target meningococci to transiently underperfused cerebral capillaries and thus determine disease development.
Published: 2006
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24. Reactive hyperaemia in the forearm skin of highly trained windsurfers

Author: François Guerrero, Jacques Mansourati, B. Simon, J.-P. Elkaïm, B. Saïag, E. Heylen, Optimisation des régulations physiologiques (ORPHY (EA 4324)), Institut Brestois Santé Agro Matière (IBSAM), and Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)
Subjects: Male, MESH: Physical Fitness, Blood Pressure, Vasodilation, 030204 cardiovascular system & hematology, Hyperaemia, 0302 clinical medicine, Heart Rate, MESH: Microcirculation, Laser-Doppler Flowmetry, Medicine, Orthopedics and Sports Medicine, MESH: Heart Rate, MESH: Laser-Doppler Flowmetry, Skin, medicine.diagnostic_test, MESH: Blood Pressure, Laser Doppler velocimetry, MESH: Systole, MESH: Case-Control Studies, MESH: Hyperemia, Forearm, medicine.anatomical_structure, Circulatory system, Cardiology, MESH: Rest, medicine.symptom, Sports, Blood vessel, Adult, medicine.medical_specialty, endothelium, Systole, Rest, laser doppler flowmetry, Hyperemia, Physical Therapy, Sports Therapy and Rehabilitation, MESH: Vasodilation, 03 medical and health sciences, MESH: Skin, Internal medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, human, MESH: Humans, business.industry, Microcirculation, MESH: Adult, Blood flow, MESH: Male, Surgery, Physical Fitness, Regional Blood Flow, Case-Control Studies, MESH: Forearm, MESH: Regional Blood Flow, MESH: Sports, business, Lipid profile, exercise training, 030217 neurology & neurosurgery
Abstract: International audience; We undertook this study to determine whether long-term high intensity exercise would modify cutaneous endothelial-dependent vasodilation. We compared a group of 9 highly trained windsurfers (mean age: 24.5 +/- 1.6 years) to a control group of 8 sedentary individuals (22.9 +/- 0.4 years, NS). Laser Doppler was used to measure cutaneous blood flow in the resting state (baseline), during post-occlusive hyperaemia (endothelium-dependent vasodilation), and local heating to 42 degrees C. Lipid profile was similar in both groups. Resting heart rate was significantly lower in windsurfers. Baseline cutaneous vascular conductance (CVC) values were similar in both groups (0.059 +/- 0.016 and 0.051 +/- 0.009). During reactive hyperaemia, normalized peak CVC value was significantly higher in the windsurfers group (1775.4 +/- 286.9 and 826.4 +/- 121.7 % baseline CVC; p = 0.01). Normalized peak CVC value in response to local heating (42 degrees C) was not significantly different between both groups (2359.4 +/- 346.1 and 1467.7 +/- 282.6 % baseline CVC). Endothelium-dependent vasodilation in cutaneous microcirculation is significantly enhanced in the forearm skin of highly trained windsurfers when compared to sedentary controls.
Published: 2005
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25. Activation of secondary auditory cortex in a deaf patient during song hallucinosis

Author: Jean-Claude Willer, Laurent D. Cohen, Lionel Naccache, Zoulika Malek, Marie-Odile Habert, Neuroimagerie cognitive (LCogn), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Imagerie Fonctionnelle (LIF), Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de médecine nucléaire [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagerie médicale et quantitative, Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), CEntre de REcherches en MAthématiques de la DEcision (CEREMADE), Centre National de la Recherche Scientifique (CNRS)-Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Physiologie et physiopathologie de la motricité chez l'homme, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR70-Institut National de la Santé et de la Recherche Médicale (INSERM), Fédération des Pathologies du Sommeil, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Service de Médecine nucléaire [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de neurologie 1 [CHU Pitié-Salpétrière], Medical imaging and robotics (EPIDAURE), Inria Sophia Antipolis - Méditerranée (CRISAM), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), INRIA Rocquencourt, and Institut National de Recherche en Informatique et en Automatique (Inria)
Subjects: medicine.medical_specialty, Neurology, Audiology, Auditory cortex, Persons With Hearing Impairments, Hallucinosis, MESH: Tomography, Emission-Computed, Single-Photon, 03 medical and health sciences, 0302 clinical medicine, medicine, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Neuroradiology, MESH: Aged, 0303 health sciences, MESH: Humans, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, MESH: Hallucinations, MESH: Hearing Impaired Persons, Hearing Impaired Persons, MESH: Regional Blood Flow, Neurology (clinical), MESH: Auditory Cortex, Psychology, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience
Published: 2005
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26. SPECT imaging, clinical features, and cognition before and after low doses of amisulpride in schizophrenic patients with the deficit syndrome

Author: Sylvie Dupont, Pierre-Michel Llorca, Michel Goudemand, Claire Rascle, Pierre Thomas, Marc Steinling, Olivier Mazas, Guillaume Vaiva, Olivier Cottencin, Patrick Devos, Wartel, Anny, Laboratoire de Neurosciences Fonctionnelles et Pathologies (LNFP), Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire Clermont-Ferrand, CHU Clermont-Ferrand, Écologie fonctionnelle et physique de l'environnement (EPHYSE - UR1263), Institut National de la Recherche Agronomique (INRA), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Cultures et Sociétés en Europe (CSE), Université Marc Bloch - Strasbourg II-Centre National de la Recherche Scientifique (CNRS), Santé publique : épidémiologie et qualité des soins-EA 2694 (CERIM), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Écologie fonctionnelle et physique de l'environnement (EPHYSE), Evaluation des technologies de santé et des pratiques médicales - ULR 2694 (METRICS), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille
Subjects: Male, MESH: Psychiatric Status Rating Scales, Perfusion scanning, Neuropsychological Tests, MESH: Schizophrenia, Disorganized, MESH: Dose-Response Relationship, Drug, 0302 clinical medicine, MESH: Treatment Outcome, Cerebral Cortex, MESH: Neuropsychological Tests, 3. Good health, Psychiatry and Mental health, Treatment Outcome, Cerebral blood flow, Frontal lobe, Schizophrenia, Anesthesia, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Amisulpride, Psychology, Antipsychotic Agents, medicine.drug, Adult, MESH: Sulpiride, medicine.drug_class, Neuroscience (miscellaneous), Atypical antipsychotic, MESH: Tomography, Emission-Computed, Single-Photon, 03 medical and health sciences, Spect imaging, medicine, Humans, Radiology, Nuclear Medicine and imaging, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Cerebral perfusion pressure, Dominance, Cerebral, Psychiatric Status Rating Scales, Tomography, Emission-Computed, Single-Photon, MESH: Humans, Dose-Response Relationship, Drug, Schizophrenia, Disorganized, MESH: Adult, MESH: Dominance, Cerebral, medicine.disease, MESH: Cerebral Cortex, MESH: Male, 030227 psychiatry, Regional Blood Flow, MESH: Antipsychotic Agents, MESH: Regional Blood Flow, Sulpiride, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; The aim of the study was to examine the action of low-dose amisulpride (100 mg/d), an atypical antipsychotic from the benzamide class with a high affinity for the D2 and D3 dopamine receptors, given for 4 weeks in 19 schizophrenic patients with the deficit syndrome, in terms of clinical response, modifications in their cognitive performance and changes in brain perfusion values. A secondary objective was to distinguish between primary and secondary deficit, according to Carpenter's definition. Both efficacy and a relatively low rate of side effects of low-dose amisulpride in the deficit forms of schizophrenia were found as expected from earlier placebo-controlled studies. Our study found significant changes in the cerebral blood flow, before and after treatment, more marked in the frontal area and particularly in the dorso-lateral frontal area. A significant improvement of cognitive function was found after treatment, without a link to any particular changes in a loco-regional perfusion value. Finally, a distinction between primary and secondary deficit showed a higher percentage of clinical improvement in the patients with a secondary deficit. The psychometric and cerebral perfusion changes were no different in the two groups.
Published: 2002

27. Functional imaging in the work-up of childhood epilepsy

Author: Olivier Dulac, András Holló, Marie Bourgeois, Pierre Vera, D. LeBihan, C. Cieuta, Anna Kaminska, Dorothée Ville, Catherine Chiron, P. F. Van de Morteele, Francis Brunelle, Lucie Hertz-Pannier, Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA), Service de neurologie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institute of Physics, Polish Academy of Sciences, Polska Akademia Nauk = Polish Academy of Sciences (PAN), Génétique et pathologie moléculaires de l'hématopoïèse, Institut National de la Santé et de la Recherche Médicale (INSERM), PSA Peugeot - Citroën (PSA), PSA Peugeot Citroën (PSA), Institut du Pin, Université Sciences et Technologies - Bordeaux 1 (UB), Service de radiologie pédiatrique [CHU Necker], Service NEUROSPIN (NEUROSPIN), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), IFR de Neuroimagerie Fonctionnelle (IFR 49), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service Hospitalier Frédéric Joliot (SHFJ), Le Bihan, Denis, Université Sciences et Technologies - Bordeaux 1, Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes ( LITIS ), Institut national des sciences appliquées Rouen Normandie ( INSA Rouen Normandie ), Normandie Université ( NU ) -Normandie Université ( NU ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Université Le Havre Normandie ( ULH ), Normandie Université ( NU ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Institute of Physics, Polish Academy of Sciences, ( PAS ), Institute of Physics, Institut National de la Santé et de la Recherche Médicale ( INSERM ), PSA Peugeot - Citroën ( PSA ), Peugeot-Citroën, Service NEUROSPIN ( NEUROSPIN ), Direction de Recherche Fondamentale (CEA) ( DRF (CEA) ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay, IFR de Neuroimagerie Fonctionnelle ( IFR 49 ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), and Service Hospitalier Frédéric Joliot ( SHFJ )
Subjects: Male, MESH : Brain Mapping, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Language Tests, MESH : Child, Preschool, Ictal-Interictal SPECT Analysis by SPM, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, Epilepsy, 0302 clinical medicine, MESH : Child, MESH: Child, Child, [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Brain Mapping, Brain Mapping, Language Tests, medicine.diagnostic_test, MESH : Epilepsy, MESH: Energy Metabolism, Brain, Cognition, MESH : Infant, MESH : Regional Blood Flow, General Medicine, MESH : Adult, Magnetic Resonance Imaging, MESH: Infant, Child, Preschool, MESH: Epilepsy, Wada test, Neurosurgery, MESH : Tomography, Emission-Computed, Single-Photon, Tomography, Emission-Computed, Adult, medicine.medical_specialty, Adolescent, MESH : Male, MESH : Language Tests, MESH: Tomography, Emission-Computed, MESH : Dominance, Cerebral, Lateralization of brain function, MESH: Tomography, Emission-Computed, Single-Photon, 03 medical and health sciences, MESH: Brain, MESH : Adolescent, MESH : Magnetic Resonance Imaging, medicine, Humans, Ictal, Dominance, Cerebral, Tomography, Emission-Computed, Single-Photon, MESH: Adolescent, MESH: Humans, business.industry, MESH : Humans, MESH: Child, Preschool, Infant, MESH: Adult, MESH: Dominance, Cerebral, medicine.disease, MESH: Male, Functional imaging, MESH : Energy Metabolism, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, MESH : Brain, Regional Blood Flow, Pediatrics, Perinatology and Child Health, MESH: Regional Blood Flow, Neurology (clinical), Nuclear medicine, business, Energy Metabolism, MESH : Tomography, Emission-Computed, Neuroscience, 030217 neurology & neurosurgery
Abstract: International audience; In children with medically intractable lesional epilepsy, surgery is deemed successful if the epileptogenic focus can be removed while major neurological functions are spared. Current techniques rely on invasive intracranial recordings. The new developments in functional imaging offer the possibility of localizing the epileptogenic focus noninvasively (PET/SPECT) and mapping cognitive functions (fMRI). Ictal SPECT shows hyperperfusion in the focus and has proved to have better localizing value than interictal PET or SPECT, which show focal hypometabolism or hypoperfusion. Ictal SPECT is useful for deciding on the placement of intracranial electrodes in extratemporal epilepsies, particularly in young children. Functional MRI has proved highly accurate for localizing motor and language networks, thus offering the possibilities of replacing the Wada test (language hemispheric lateralization) and studying postlesional brain plasticity. Despite the difficulties of functional imaging in children owing to the limited cooperation that can be expected, ethical constraints, and poor normative data, SPECT/PET and fMRI provide clinically useful information for presurgical work-up of childhood epilepsies.
Published: 2001

28. Prediction of tissue survival after middle cerebral artery occlusion based on changes in the apparent diffusion of water

Author: Saburo Sakaki, Masaharu Sakoh, Donald F. Smith, Lisbeth Røhl, Carsten Gyldensted, Albert Gjedde, Leif Østergaard, Peter Vestergaard-Poulsen, Denis Le Bihan, Le Bihan, Denis, ISR Division, Los Alamos National Laboratory (LANL), 7 Brodick Drive, Service NEUROSPIN (NEUROSPIN), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, IFR de Neuroimagerie Fonctionnelle (IFR 49), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Subjects: Blood Glucose, Swine, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Hemodynamics, Infarction, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, Diffusion, MESH: Blood-Brain Barrier, 0302 clinical medicine, MESH: Animals, MESH: Oxygen Consumption, MESH: Swine, medicine.diagnostic_test, Cerebral infarction, MESH: Energy Metabolism, Brain, MESH: Infarction, Middle Cerebral Artery, Infarction, Middle Cerebral Artery, MESH: Diffusion, Magnetic Resonance Imaging, Cerebral blood flow, Blood-Brain Barrier, Reperfusion Injury, MESH: Image Enhancement, Tomography, Emission-Computed, MESH: Tomography, Emission-Computed, Ischemia, 03 medical and health sciences, MESH: Brain, Oxygen Consumption, medicine, Effective diffusion coefficient, Animals, MESH: Tissue Survival, Dominance, Cerebral, Tissue Survival, business.industry, Magnetic resonance imaging, MESH: Dominance, Cerebral, medicine.disease, Image Enhancement, body regions, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, Regional Blood Flow, MESH: Blood Glucose, MESH: Regional Blood Flow, MESH: Reperfusion Injury, Nuclear medicine, business, Energy Metabolism, 030217 neurology & neurosurgery, Diffusion MRI
Abstract: Object. In this study the authors tested the hypothesis that the estimate of the apparent diffusion coefficient (ADC) of water is a reliable pathophysiological index of the viability of ischemic brain tissue. Methods. Cerebral blood flow (CBF) and the cerebral metabolic rates of oxygen and glucose (CMRO2 and CMRglc, respectively) were measured using positron emission tomography (PET) scanning before and after permanent middle cerebral artery occlusion (MCAO) or reperfusion in pigs. The ADC value, which was measured using diffusion-weighted magnetic resonance (DW MR) imaging was compared with physiological variables obtained by PET scanning and with histological findings. After both permanent MCAO and reperfusion, the decrease in the ADC was significantly correlated with decrease in the CMRO2 and CMRglc. The infarction coincided with a CMRO2 threshold of 50% of the value measured on the contralateral side. Thus, an ADC value of 80% or 75% of the contralateral value reflected the CMRO2 threshold after permanent MCAO or reperfusion, respectively. On DW MR images, lesions with ADC values above 80% of the contralateral value are potentially reversible until 6 hours after MCAO, whereas lesions with ADC values below 75% of the contralateral value are irreversible as early as 2 hours after MCAO. Conclusions. The ADC of water provides a reliable pathophysiological index for tailoring therapy to the condition of individual stroke patients in clinical practice.
Published: 2001

29. Inter-organ substrate exchanges in the critically ill

Author: Xavier Leverve, Hamant, Sarah, Bioénergétique fondamentale et appliquée, and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: MESH: Oxidation-Reduction, MESH: Hydrogen-Ion Concentration, MESH: Amino Acids, Medicine (miscellaneous), Kidney, Intestinal absorption, 0302 clinical medicine, Protein biosynthesis, Homeostasis, Amino Acids, MESH: Multiple Organ Failure, MESH: Nitrogen, chemistry.chemical_classification, 0303 health sciences, MESH: Muscle, Skeletal, Nutrition and Dietetics, ATP synthase, biology, Chemistry, Substrate (biology), Hydrogen-Ion Concentration, Cell biology, Amino acid, Perfusion, MESH: Glucose, medicine.anatomical_structure, Liver, MESH: Homeostasis, MESH: Critical Illness, Oxidation-Reduction, MESH: Perfusion, Nitrogen, Critical Illness, Multiple Organ Failure, MESH: Digestive System, 03 medical and health sciences, Albumins, medicine, Humans, Muscle, Skeletal, 030304 developmental biology, MESH: Humans, Albumin, MESH: Kidney, Glucose, Regional Blood Flow, biology.protein, MESH: Regional Blood Flow, MESH: Albumins, Digestive System, 030217 neurology & neurosurgery, MESH: Liver
Abstract: International audience; Metabolic inter-organ exchange is a major field of research for improving the treatment of the critically ill. Adapting regional blood flows is the first regulatory step, although the relationships between hypoperfusion and metabolic disorders are matter of controversy. Metabolic steady state results from a vast inter-organ interplay and several nutrients or metabolites are signalling molecules in the regulation of gene transcription. Inter- or intra-organ substrate recycling shares or delays the mandatory need for aerobic ATP synthesis in some conditions. Nitrogen metabolism is highly compartmentalised in an inter-organ co-operation and liver, muscle, kidney and gut are the most important organs. By remodelling the amino acid mixture delivered to peripheral cells after intestinal absorption, the liver plays a determinant role in whole body protein synthesis. Albumin turnover increases after brain injury. Since the location of synthesis is different to that of breakdown this turnover can be viewed as an inter-organ exchange. The metabolic side of pH homeostasis is also an inter-organ exchange mainly shared by liver, kidney and muscle.
Published: 2001

30. From tissue perfusion to metabolic marker: assessing organ competition and co-operation in critically ill patients?

Author: Xavier Leverve, Hamant, Sarah, Bioénergétique fondamentale et appliquée, and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: medicine.medical_specialty, Pain medicine, Critical Care and Intensive Care Medicine, Co operation, Competition (economics), 03 medical and health sciences, 0302 clinical medicine, Anesthesiology, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Medicine, Metabolic Marker, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030212 general & internal medicine, Intensive care medicine, ComputingMilieux_MISCELLANEOUS, MESH: Humans, business.industry, Critically ill, MESH: Energy Metabolism, MESH: Biological Markers, 030208 emergency & critical care medicine, 3. Good health, MESH: Homeostasis, MESH: Critical Illness, MESH: Regional Blood Flow, business, Perfusion
Abstract: International audience
Published: 1999

31. Human taste cortical areas studied with functional magnetic resonance imaging: evidence of functional lateralization related to handedness

Author: P. Mac Leod, Annick Faurion, D. Le Bihan, P. F. Van De Moortele, E. Lobel, B. Cerf, Le Bihan, Denis, Danone Research, Groupe DANONE, Service NEUROSPIN (NEUROSPIN), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), IFR de Neuroimagerie Fonctionnelle (IFR 49), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Subjects: Adult, MESH: Hemodynamics, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Central nervous system, Synaptic Transmission, 050105 experimental psychology, Functional Laterality, MESH: Magnetic Resonance Imaging, 03 medical and health sciences, 0302 clinical medicine, Tongue, Functional neuroimaging, MESH: Stimulation, Chemical, medicine, MESH: Synaptic Transmission, Humans, 0501 psychology and cognitive sciences, MESH: Functional Laterality, Dominance, Cerebral, Cerebral Cortex, MESH: Humans, medicine.diagnostic_test, General Neuroscience, 05 social sciences, MESH: Tongue, Hemodynamics, MESH: Adult, MESH: Dominance, Cerebral, Magnetic Resonance Imaging, Stimulation, Chemical, MESH: Cerebral Cortex, Electrophysiology, medicine.anatomical_structure, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, Cerebral cortex, Regional Blood Flow, Taste, Laterality, MESH: Taste, MESH: Regional Blood Flow, Functional magnetic resonance imaging, Psychology, Insula, Neuroscience, 030217 neurology & neurosurgery
Abstract: International audience; Whole-brain functional magnetic resonance imaging was used to detect local hemodynamic changes reflecting cortical activation in five left handed and five right handed human subjects during bilateral stimulation of the tongue with various tastes. Activation was found bilaterally in the insula and the perisylvian region. These regions correspond to the primary taste cortical areas identified with electrophysiological recordings in monkeys and suggested from former clinical observations in human subjects. Moreover, a unilateral projection was described for the first time in the inferior part of the insula of the dominant hemisphere, according to the subject's handedness.
Published: 1999

32. Importance of initial coronary artery flow after heart procurement to assess heart viability before transplantation

Author: R. Forrat, P. Marcsek, M de Lorgeril, Georges Dureau, René Ferrera, De Lorgeril, Michel, Cardioprotection, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)
Subjects: Male, Hot Temperature, Swine, 030204 cardiovascular system & hematology, 0302 clinical medicine, Ischemia, Edema, MESH: Sodium, Medicine, MESH: Animals, MESH: Swine, Edema, Cardiac, Graft Survival, MESH: Heart Transplantation, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, medicine.anatomical_structure, MESH: Calcium, 030220 oncology & carcinogenesis, Anesthesia, Heart Arrest, Induced, Female, Tissue Preservation, medicine.symptom, Cardiology and Cardiovascular Medicine, Artery, MESH: Edema, Cardiac, MESH: Myocardium, MESH: Graft Survival, Sodium, MESH: Tissue Preservation, chemistry.chemical_element, MESH: Coronary Circulation, MESH: Hot Temperature, 03 medical and health sciences, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Coronary Circulation, Physiology (medical), Animals, Coronary sinus, business.industry, Myocardium, Blood flow, medicine.disease, MESH: Male, Transplantation, chemistry, Regional Blood Flow, Ventricle, MESH: Potassium, Potassium, Heart Transplantation, MESH: Regional Blood Flow, MESH: Heart Arrest, Induced, Calcium, MESH: Ischemia, business, MESH: Female
Abstract: Background The objective of this study was to evaluate different tests of heart viability in a pig model of warm ischemia. Methods and Results Pig hearts (n=30) were submitted to 0 (= group I), 10 (group II), 20 (group III), 30 (group IV), and 60 (group V) minutes of in situ warm ischemia (animal exsanguination). Hearts were removed, then flushed with cardioplegic solution for 3 minutes at a fixed pressure of 60 cm H 2 O, and edema formation, initial coronary flow, and ionic composition (Na + , K + , and Ca ++ ) of coronary sinus effluent were evaluated. Hearts were then stored for 2 hours in a cold (4°C) preservation solution. Myocardial biopsies (and evaluation of energetic index) were performed, then the hearts were reperfused for 30 minutes with whole blood with an in vitro functional testing system. No edema occurred during cardioplegic flush in the hearts in groups I through IV, but a 37±11% weight increase ( P P P r =.84, P r =.90, P P P P Conclusions These data suggest that early measurement of coronary flow after removal of the heart may help to assess heart viability before transplantation. This approach may provide a comprehensive clinical evaluation to increase the number of hearts available for transplantation among those that are rejected in the absence of accurate criteria of viability.
Published: 1995

33. Cerebral blood flow in major depression and dysthymia

Author: Guillaume Vaiva, Marc Steinling, Michel Goudemand, C. Alaix, Pierre Thomas, E. Samaille, M. Maron, Laboratoire de Neurosciences Fonctionnelles et Pathologies (LNFP), Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), and Wartel, Anny
Subjects: Male, MESH: Depressive Disorder, Hemodynamics, Single photon emission computerized tomography, MESH: Frontal Lobe, MESH: Technetium Tc 99m Exametazime, 0302 clinical medicine, Oximes, Frontal region, Major depressive episode, Depression (differential diagnoses), MESH: Brain Mapping, Cerebral Cortex, Brain Mapping, MESH: Middle Aged, medicine.diagnostic_test, Brain, Organotechnetium Compounds, Middle Aged, MESH: Oximes, 3. Good health, Frontal Lobe, Psychiatry and Mental health, Clinical Psychology, Cerebral blood flow, Positron emission tomography, Anesthesia, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], medicine.symptom, Psychology, Perfusion, Adult, MESH: Tomography, Emission-Computed, Single-Photon, 03 medical and health sciences, MESH: Brain, Technetium Tc 99m Exametazime, MESH: Organotechnetium Compounds, medicine, Humans, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Dominance, Cerebral, Tomography, Emission-Computed, Single-Photon, Depressive Disorder, MESH: Humans, MESH: Adult, MESH: Dominance, Cerebral, MESH: Cerebral Cortex, MESH: Male, 030227 psychiatry, Regional Blood Flow, MESH: Regional Blood Flow, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; Using single photon emission computerized tomography (SPECT) with a 99mTc-HMPAO perfusion technique, we studied the regional cerebral blood flow (rCBF) of 42 drug-free inpatients suffering from Major Depression' (n = 21) or dysthymia with the super-imposed diagnosis of a major depressive episode (n = 21). The patients with Major Depression had a significantly lower frontal and posterior rCBF ratio than those with Double Depression. Left frontal region indices showed a slight overlap between the two groups. There was no correlation between the severity of the illness and the rCBF indices. Different qualitative cerebral dysfunctions may be implicated in these two affective disorder sub-types.
Published: 1993

34. Structure-function relation in the coronary artery tree: From fluid dynamics to arterial bifurcations

Author: Jacques Ohayon, Yunlong Huo, Patrice Guerin, Gilles Rioufol, Ghassan S. Kassab, Gérard Finet, Department of Hemodynamics and Interventional Cardiology, Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Cardiovasculaire Louis Pradel, Hospices Civils de Lyon (HCL), Dynacell, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)
Subjects: MESH: Models, Cardiovascular, MESH: Hemodynamics, [SDV]Life Sciences [q-bio], 0206 medical engineering, Treatment outcome, MESH: Coronary Circulation, Coronary Artery Disease, 02 engineering and technology, 030204 cardiovascular system & hematology, MESH: Hydrodynamics, 03 medical and health sciences, 0302 clinical medicine, Coronary Circulation, Animals, Humans, Medicine, MESH: Coronary Vessels, MESH: Animals, MESH: Coronary Artery Disease, Angioplasty, Balloon, Coronary, ComputingMilieux_MISCELLANEOUS, MESH: Treatment Outcome, MESH: Stress, Mechanical, MESH: Fractals, MESH: Humans, business.industry, Structure function, Hemodynamics, Models, Cardiovascular, Claude bernard, MESH: Blood Flow Velocity, Coronary Vessels, 020601 biomedical engineering, 3. Good health, MESH: Angioplasty, Balloon, Coronary, MESH: Stents, Fractals, Treatment Outcome, Regional Blood Flow, Hydrodynamics, Stents, MESH: Regional Blood Flow, Stress, Mechanical, Cardiology and Cardiovascular Medicine, business, Humanities, Blood Flow Velocity
Abstract: 1. Departement of Interventional Cardiology, Cardiovascular Hospital and Claude Bernard University, and INSERM 886, Lyon, France; 2. Department of Biomedical Engineering, Indiana-Purdue University, Indianapolis, IN, USA; 3. Laboratory TIMC-IMAG, DynaCell, CNRS UMR 5525, Institut de l’Ingenierie et de l’Information de Sante (In3S), Grenoble, France; 4. INSERM UMR915, Institut du Thorax, Service d’Hemodynamique, and Centre Commun de Microscopie Electronique, Nantes, France
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34 results on '"MESH: Regional Blood Flow"'

1. Endothelial autophagic flux hampers atherosclerotic lesion development

2. The Accuracy of Noninvasive Hemoglobin Measurement by Multiwavelength Pulse Oximetry After Cardiac Surgery

3. Cerebral autoregulation in patients with obstructive sleep apnea syndrome during wakefulness

4. Circulating Vascular Cell Adhesion Molecule-1 Is Associated With Cerebral Blood Flow Dysregulation, Mobility Impairment, and Falls in Older Adults

5. A novel device for measuring arterial stiffness using finger-toe pulse wave velocity: Validation study of the pOpmètre®

6. Bilateral thenar hammer syndrome

7. Disseminated arterial calcification and enhanced myogenic response are associated with abcc6 deficiency in a mouse model of pseudoxanthoma elasticum

8. Effect of Chronic Severe Diabetes on Myocardial Stunning in the Dog

9. Cutaneous microvascular functional assessment during exercise: a novel approach using laser speckle contrast imaging

10. A simulation tool for dynamic contrast enhanced MRI

11. Primary liver cancer is more aggressive in HIV-HCV coinfection than in HCV infection. A prospective study (ANRS CO13 Hepavih and CO12 Cirvir)

12. Sildenafil increases digital skin blood flow during all phases of local cooling in primary Raynaud's phenomenon

13. Normal muscle oxygen consumption and fatigability in sickle cell patients despite reduced microvascular oxygenation and hemorheological abnormalities

14. Perfusion MR imaging and 1H spectroscopy: their role in the diagnosis of microcystic and lipomatous meningiomas

15. Theoretical Principles of Perfusion Imaging: Application to Magnetic Resonance Imaging

16. Unilateral blood flow decrease induces bilateral and symmetric responses in the immature brain

17. Alteration of endothelium-mediated vasodilator response in the rat hindlimb vasculature consecutive to chronic hypoxic stress: NO and EDHF involvement

18. Correlation of biomarkers of endothelium dysfunction and matrix remodeling in patients with systemic sclerosis

19. Prevention of skin flap necrosis by estradiol involves reperfusion of a protected vascular network

20. Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen

21. Atrial natriuretic peptide contribution to lipid mobilization and utilization during head-down bed rest in humans

22. Paradoxical role of angiotensin II type 2 receptors in resistance arteries of old rats

23. Cerebral microcirculation shear stress levels determine Neisseria meningitidis attachment sites along the blood–brain barrier

24. Reactive hyperaemia in the forearm skin of highly trained windsurfers

25. Activation of secondary auditory cortex in a deaf patient during song hallucinosis

26. SPECT imaging, clinical features, and cognition before and after low doses of amisulpride in schizophrenic patients with the deficit syndrome

27. Functional imaging in the work-up of childhood epilepsy

28. Prediction of tissue survival after middle cerebral artery occlusion based on changes in the apparent diffusion of water

29. Inter-organ substrate exchanges in the critically ill

30. From tissue perfusion to metabolic marker: assessing organ competition and co-operation in critically ill patients?

31. Human taste cortical areas studied with functional magnetic resonance imaging: evidence of functional lateralization related to handedness

32. Importance of initial coronary artery flow after heart procurement to assess heart viability before transplantation

33. Cerebral blood flow in major depression and dysthymia

34. Structure-function relation in the coronary artery tree: From fluid dynamics to arterial bifurcations

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34 results on '"MESH: Regional Blood Flow"'

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