11 results on '"Kylie Taylor"'
Search Results
2. Selection and validation of reference genes for normalisation of gene expression in ischaemic and toxicological studies in kidney disease.
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Sanjeeva Herath, Hongying Dai, Jonathan Erlich, Amy Ym Au, Kylie Taylor, Lena Succar, and Zoltán H Endre
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Medicine ,Science - Abstract
Normalisation to standard reference gene(s) is essential for quantitative real-time polymerase chain reaction (RT-qPCR) to obtain reproducible and comparable results of a gene of interest (GOI) between subjects and under varying experimental conditions. There is limited evidence to support selection of the commonly used reference genes in rat ischaemic and toxicological kidney models. Employing these models, we determined the most stable reference genes by comparing 4 standard methods (NormFinder, qBase+, BestKeeper and comparative ΔCq) and developed a new 3-way linear mixed-effects model for evaluation of reference gene stability. This new technique utilises the intra-class correlation coefficient as the stability measure for multiple continuous and categorical covariates when determining the optimum normalisation factor. The model also determines confidence intervals for each candidate normalisation gene to facilitate selection and allow sample size calculation for designing experiments to identify reference genes. Of the 10 candidate reference genes tested, the geometric mean of polyadenylate-binding nuclear protein 1 (PABPN1) and beta-actin (ACTB) was the most stable reference combination. In contrast, commonly used ribosomal 18S and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were the most unstable. We compared the use of PABPN1×ACTB and 2 commonly used genes 18S and GAPDH on the expression of 4 genes of interest know to vary after renal injury and expressed by different kidney cell types (KIM-1, HIF1α, TGFβ1 and PECAM1). The less stable reference genes gave varying patterns of GOI expression in contrast to the use of the least unstable reference PABPN1×ACTB combination; this improved detection of differences in gene expression between experimental groups. Reduced within-group variation of the now more accurately normalised GOI may allow for reduced experimental group size particularly for comparison between various models. This objective selection of stable reference genes increased the reliability of comparisons within and between experimental groups.
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- 2020
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3. Invasive pneumococcal disease in New South Wales, Australia: reporting Aboriginal and Torres Strait Islander status improves epidemiology
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David N Durrheim, Kylie Taylor, Maggi Osbourn, Kerry Todd, and Peter D Massey
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Pneumococcal disease ,surveillance ,Aboriginal ,Torres Strait Islander ,epidemiology ,Australia ,Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
The aim of this work was to determine the feasibility of improving Aboriginal and Torres Strait Islander status recording for notifiable diseases using all Invasive Pneumococcal Disease (IPD) notifications in a regional area of New South Wales, Australia.In Australia people with IPD are nearly always admitted to hospital and their Aboriginal and Torres Strait Islander status is recorded. Aboriginal and Torres Strait Islander status was determined for IPD notifications by referring to the routine hospital admission data, in a regional area of New South Wales, Australia.There were 234 notifications in the regional area of Hunter New England during the period 2007–2009. Initially, 168 (72%) notifications had Aboriginal and Torres Strait Islander status recorded. After referring to the routine hospital admission data the recorded status increased to 232 (99%). Updating the surveillance data required less than five minutes per notification.Referring to routine hospital admission data proved a useful and time-efficient surveillance strategy to increase the proportion of notifications with Aboriginal and Torres Strait Islander status. These data can then be used to better understand the current epidemiology of IPD. Aboriginal and Torres Strait Islander children aged 0–4 years have a two- to threefold higher rate of invasive pneumococcal disease than non-Aboriginal children, thus high levels of timely pneumococcal immunization coverage remain important for young Aboriginal and Torres Strait Islander children.
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- 2011
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4. Pediatric Emergency Telehealth
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Ameer Mody and Kylie Taylor
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Pediatric emergency ,business.industry ,medicine ,Telehealth ,Medical emergency ,medicine.disease ,business - Abstract
Pediatric Emergency Telehealth (PET) has enormous potential to help address the health care needs of children and families. In some regions, health care is particularly fragmented or difficult to access for the pediatric patient. Telehealth may bridge those gaps, improve continuity of care, and enhance communication between children’s hospitals and general pediatricians practicing in both rural and urban communities. Launching a PET service requires careful preparation with community health networks and stakeholders to ensure that the model is providing adjunct services as opposed to on-demand primary care services. General pediatricians’ offices that lack the capability to implement a virtual care system may seek consultation from the PET service in terms of triage, recommendations, or direct patient care. A substantial effort is required at initiation of a PET program but is useful in mitigating disease exposure such as Covid-19, as well as improving emergency department metrics such as patient satisfaction, length of stay, wait times, and revenue capture.
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- 2021
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5. Describing meningococcal disease: understanding, perceptions and feelings of people in a regional area of NSW, Australia
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Julie Kohlhagen, Kylie Taylor, Maggi Osbourn, Peter D Massey, and Myfanwy Maple
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Adult ,Male ,Health Knowledge, Attitudes, Practice ,medicine.medical_specialty ,Adolescent ,media_common.quotation_subject ,Empathy ,Disease ,Meningococcal disease ,Racism ,Interviews as Topic ,Young Adult ,Nursing ,Perception ,medicine ,Humans ,media_common ,business.industry ,Health Policy ,Public health ,Public Health, Environmental and Occupational Health ,Focus Groups ,Middle Aged ,medicine.disease ,Focus group ,Meningococcal Infections ,Feeling ,Female ,New South Wales ,business ,Attitude to Health - Abstract
Objectives: To explore understanding, perceptions and feelings about meningococcal disease in members of higher risk groups. To explore what people say are the most important health messages and communication preferences about invasive meningococcal disease (IMD). Methods: Three focus groups and two semistructured interviews were conducted with people at higher risk of IMD in Hunter New England Local Health District in New South Wales. Results: Participants generally had a low understanding of IMD, but described intense feelings about the disease and empathy for those who had experienced the disease. Fear of stigma and the impact of stigma were identified. Participants identified reasons for delaying presentation for care as perceptions of invincibility (particularly among young people), the cost of care (for all groups), and racism (particularly for Aboriginal people). These issues were both potential and experienced barriers for participants accessing help when acutely unwell. Factors for effective communication to improve understanding of IMD included the communication being acceptable, accessible and appropriate. Conclusions: IMD is a serious but uncommon disease that has a range of impacts on people, families and communities. Higher risk groups may benefit from receiving more appropriate and accessible information about early signs and symptoms of IMD. Communication and understanding about the disease could be improved by working with new technologies and partnering with key people in high-risk groups. Use of text messages and social networking for urgent communication could be considered and trialled in public health practice. It is also important to recognise the potential direct or indirect experience of racism and stigma for patients with IMD and their families. Management of IMD could be strengthened by connecting people and families with support groups or services to reduce the impact of the disease.
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- 2016
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6. Systematic literature review to identify methods for treating and preventing bacterial skin infections in Indigenous children
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Richard C. Franklin, Kylie Taylor, Smriti Nepal, Peter D Massey, and Susan Thomas
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medicine.medical_specialty ,Veterinary medicine ,Native Hawaiian or Other Pacific Islander ,Adolescent ,Alternative medicine ,MEDLINE ,Dermatology ,CINAHL ,Skin infection ,Indigenous ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Swimming Pools ,Personal hygiene ,030225 pediatrics ,medicine ,Humans ,030212 general & internal medicine ,Toilet Facilities ,Child ,business.industry ,Australia ,Infant, Newborn ,Infant ,Hygiene ,Skin Diseases, Bacterial ,medicine.disease ,Anti-Bacterial Agents ,Systematic review ,Family medicine ,Child, Preschool ,Inclusion and exclusion criteria ,Housing ,business - Abstract
Background/Objectives: Bacterial skin infections in Indigenous children in Australia frequently lead them to access primary health care. This systematic review aims to identify and analyse available studies describing the treatment and prevention of bacterial skin infections in Indigenous children. Methods: Electronic databases including Scopus, MEDLINE, CINAHL, ProQuest, Informit and Google Scholar were searched. Studies in English published between August 1994 and September 2016, with the subject of bacterial skin infections involving Indigenous children and conducted in Australia, New Zealand, the USA or Canada were selected. Results: Initially 1474 articles were identified. After the application of inclusion and exclusion criteria, 10 articles remained. Strategies for the treatment and prevention of bacterial skin infections included the management of active infections and lesions, improving environmental and personal hygiene, the installation of swimming pools and screening and treatment. Conclusion: There is a need for more, rigorous, large-scale studies to develop evidence for appropriate, culturally acceptable methods to prevent and manage bacterial skin infections in Indigenous children in Australia. The problem is complex with multiple determinants. Until underlying socioeconomic conditions are addressed skin infections will continue to be a burden to communities.
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- 2016
7. Reducing recurrence of bacterial skin infections in Aboriginal children in rural communities: new ways of thinking, new ways of working
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Ruth Williams, Peter D Massey, Kylie Taylor, Susan Thomas, Kristy Crooks, and Glenn Pearce
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Male ,Rural Population ,medicine.medical_specialty ,Native Hawaiian or Other Pacific Islander ,Adolescent ,Participatory action research ,Population health ,03 medical and health sciences ,0302 clinical medicine ,Quality of life (healthcare) ,Nursing ,Recurrence ,Health care ,Humans ,Medicine ,030212 general & internal medicine ,Social determinants of health ,Child ,Qualitative Research ,030505 public health ,business.industry ,Health Policy ,Public health ,Public Health, Environmental and Occupational Health ,Skin Diseases, Bacterial ,Focus Groups ,Focus group ,Child, Preschool ,Community health ,Quality of Life ,Female ,Health Services Research ,New South Wales ,0305 other medical science ,business - Abstract
Reports from health workers, school staff and community members in rural NSW suggested that bacterial skin infections are a significant health issue for Aboriginal children and their families, affecting quality of life and contributing to poor school attendance. Current NSW treatment guidelines do not incorporate important sociocultural factors or ways of living in Aboriginal communities. The aim of this qualitative study was to gain a deeper understanding of the experience of parents and carers of Aboriginal children affected by skin infections and of other community members, health workers and school staff, and what actions have been considered successful or unsuccessful in reducing the recurrence of infection. This study used a Participatory Action Research methodology. Interviews and focus groups were conducted with 38 health workers and managers, school staff, community members and parents and carers. Themes that emerged included: (i) skin infections have become normalised; (ii) skin infections are, in part, a consequence of transgenerational trauma; (iii) skin infections are interwoven with social determinants; (iv) families have survived but more could thrive; and (v) something can and should be done about the problem. The findings of this study will inform the development of more effective and acceptable options to reduce skin infections in Aboriginal children.
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- 2017
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8. 'Makes you proud to be black eh?': Reflections on meaningful Indigenous research participation
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Travis Odo, Susan Reilly, Glenn Pearce, Jenni Judd, Jenny Kelly, Rosita Billycan, Jennifer Bull, Peter D Massey, Shayne Ahboo, Sherry Saggers, John Thomas, and Kylie Taylor
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Adult ,Male ,Torres Strait Islander ,Community-Based Participatory Research ,Health Knowledge, Attitudes, Practice ,Native Hawaiian or Other Pacific Islander ,Participatory action research ,Community-based participatory research ,Indigenous ,Education ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,030212 general & internal medicine ,Aboriginal ,Health policy ,Social policy ,Motivation ,business.industry ,Research ,Health Policy ,lcsh:Public aspects of medicine ,05 social sciences ,Australia ,Public Health, Environmental and Occupational Health ,Health services research ,Participation ,050301 education ,lcsh:RA1-1270 ,Middle Aged ,Public relations ,General partnership ,Female ,business ,0503 education ,Qualitative research - Abstract
Introduction This article outlines the meaningful participation of eight Aboriginal and Torres Strait Islander community members employed as community researchers investigating the impact of pandemic influenza in rural and remote Indigenous communities in Australia. Aboriginal and Torres Strait Islander participation is now a requirement of health research involving Aboriginal and Torres Strait Islander communities. There is a growing literature on the different approaches to such involvement. Fundamental to this literature is an acknowledgement that Indigenous communities are no longer prepared to be research objects for external, mostly non-Indigenous researchers, and demand a role in decisions about what is researched and how it will be researched. In this paper, we describe the protracted process for site identification and recruitment and training of community researchers. We focus on the backgrounds of the Indigenous researchers and their motivations for involvement, and the strengths and challenges posed by Indigenous people researching in their own communities. Throughout the paper our concern is to document how genuine participation and the building of research capacity can occur. Discussion A key feature of the research was the employment, training and strengthening the capacity of local Aboriginal and Torres Strait Islander community members in the role of community researchers. A series of training workshops were conducted in northern Australia and focussed on qualitative research methods, including data collection, data analysis and writing. The Indigenous researchers collected the community-based data, and worked in partnership with experienced academic researchers in the analysis and compilation of community reports. Parts of those community reports, as well as additional information supplied by the community researchers, forms the basis of this article. As the demand increases for involvement of Indigenous community members as researchers, focus needs to be paid to what constitutes meaningful participation. If active participation in all aspects of the research process is intended, this necessitates close attention to the knowledge and skills required for this to occur at every stage. Building research capacity means not simply equipping local people to undertake research on a particular project, but to have the knowledge and skills to undertake research in other areas. Conclusions There are considerable benefits for Indigenous people researching in their own communities. Most important for the community researchers on this project was the sense that they were doing important health work, not just conducting research. Given the persistent gaps between Indigenous and non-Indigenous health, this is perhaps one of the most important contributions of this type of research. Whilst research outcomes are undoubtedly important, in many cases the process used is of greater importance.
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- 2012
9. Australian Aboriginal and Torres Strait Islander communities and the development of pandemic influenza containment strategies: community voices and community control
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Kylie Taylor, Jenni Judd, Adrian Miller, Jennifer Broome, Peter D Massey, David N Durrheim, Jenny Kelly, Travis Odo, Sherry Saggers, Glenn Pearce, Alan R. Clough, Richard Speare, and Magdalena Blackley
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medicine.medical_specialty ,Community-Based Participatory Research ,Native Hawaiian or Other Pacific Islander ,media_common.quotation_subject ,Participatory action research ,Community-based participatory research ,Indigenous ,Influenza A Virus, H1N1 Subtype ,Pandemic ,Influenza, Human ,Medicine ,Humans ,Pandemics ,media_common ,Government ,Distrust ,business.industry ,Health Policy ,Public health ,Media studies ,Community Participation ,Public relations ,Work (electrical) ,Communicable Disease Control ,Queensland ,business - Abstract
Objectives To develop culturally appropriate and effective strategies to reduce the risk from pandemic influenza (H1N109) in rural and remote Australian Aboriginal and Torres Strait Islander communities. Methods Participatory Action Research (PAR) approach that enabled communities and researchers to work together to develop understanding and take action to reduce risk. Results The H1N109 pandemic raised deep concerns and serious issues in all of the Aboriginal and Torres Strait Islander communities involved in this project. The participants expressed distrust and scepticism in relation to current Australian health policies on containment and told the researchers that specific plans for Aboriginal and Torres Strait Islander peoples were needed. Respondents indicated that policies and plans had been developed without respectful engagement with communities. The strong and recurring themes that emerged from the PAR cycles were: the importance of family; ways of life and realities of living in response to influenza; and key messages to government and health services to focus on communication, understanding and respect. Conclusion The essential work of reducing risk of pandemic influenza with Aboriginal and Torres Strait Islander communities is not straightforward, but this project has highlighted a number of useful pathways to continue to journey along with communities. A number of strategies to reduce the spread of pandemic influenza in Aboriginal and Torres Strait Islander communities were identified. These strategies would make a good starting point for conversations with communities and health services. In Aboriginal and Torres Strait Islander communities the environment, community structures and traditions vary. Respectful engagement with communities is needed to develop effective policy.
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- 2010
10. Reducing the risk of pandemic influenza in Aboriginal communities
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Peter D Massey, David N Durrheim, Kylie Taylor, Sherry Saggers, Glenn Pearce, and Lisa Orcher
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Emergency Medical Services ,Veterinary medicine ,Health (social science) ,business.industry ,Public Health, Environmental and Occupational Health ,Medicine (miscellaneous) ,Context (language use) ,Public relations ,Focus group ,Indigenous ,Social support ,Need to know ,General partnership ,Pandemic ,Medicine ,business ,Risk management - Abstract
Context Aboriginal people are particularly vulnerable to pandemic influenza A, H1N109. This was first recognized in the First Nations of Canada. There have been calls for close planning with Aboriginal people to manage these risks. This article describes the process and findings from preliminary community consultations into reducing influenza risk, including pandemic H1N1(09) swine influenza, in Aboriginal communities in the Hunter New England area of northern New South Wales, Australia. Issue Consultation was conducted with 6 Aboriginal communities in response to the rapidly evolving pandemic and was designed to further develop shared understanding between health services and Aboriginal communities about appropriate and culturally safe ways to reduce the influenza risk in communities. Agreed risk mitigation measures identified in partnership are being introduced throughout Hunter New England area. Lessons learned Five theme areas were identified that posed particular challenges to limiting the negative impact of pandemic influenza; and a number of potential solutions emerged from focus group discussions: (1) local resource person: local identified 'go to' people are heard and trusted, but need to have an understanding of H1N109; (2) clear communication: information must be presented simply, clearly and demonstrating respect for local culture; (3) access to health services: sick people need to know where to get help and how to get there without infecting others; (4) households and funerals: infection control messages should be aligned with the reality of life in Aboriginal communities, and the importance of attending family and cultural gatherings; (5) social and community support issues: Aboriginal people need to have a say in how support is provided. Influenza pandemics are a serious threat to the health and social functioning of Aboriginal communities. Measures to reduce the risk of influenza in communities must be developed with the communities to maximise their acceptance. The process of engagement and ongoing respectful negotiations with communities is critical to developing culturally appropriate pandemic mitigation and management strategies.
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- 2009
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11. Haemolytic uraemic syndrome: A cluster of cases in early 1999
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Greg Bell, Elizabeth J Smedley, Tass Karalis, Stephen Crone, Kylie Taylor, Patrick Maywood, Leena Gupta, Lorraine C Young, Des White, Darlene Pennel, Valerie Delpech, Michael Staff, Mark Bartlett, Jeremy McAnulty, and Alison Rutherford
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Pediatrics ,medicine.medical_specialty ,business.industry ,hemic and lymphatic diseases ,Incidence (epidemiology) ,Public Health, Environmental and Occupational Health ,Medicine ,Haemolytic-uraemic syndrome ,urologic and male genital diseases ,business ,Disease cluster ,female genital diseases and pregnancy complications - Abstract
Haemolytic uraemic syndrome (HUS), an illness with potentially serious sequelae, is reported infrequently in Australia. This article summarises the limited NSW data available describing cases of HUS and outlines an investigation into an apparent increase in incidence of HUS in NSW in the first quarter of 1999.
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- 1999
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