Search

Your search keyword '"Komal Salkar"' showing total 2 results
Start Over Author "Komal Salkar" Topic medicine
2 results on '"Komal Salkar"'

1. Comparative assessment of amino acids composition in two types of marine fish silage

Author

Mukund Gauthankar, Rakhee Khandeparker, Mamatha S. Shivaramu, Komal Salkar, Rayadurga Anantha Sreepada, and Mandar Paingankar

Subjects
Medicine, Science
Abstract

Abstract Fish silage is a brown liquefied product achieved by the action of enzymes when finely grounded whole/parts of either single or mixed fish types are subjected to acidification. This study made a comparative assessment of biochemical and nutritive properties, especially the amino acid composition in supernatant phase of formic acid silages prepared from two fish types, Indian mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat content > 5%) and lean fish (LF, fat content

Published
2021
Full Text
View/download PDF

2. LISTERIA MONOCYTOGENES STRAIN BN3 RESPONDS TO ACYL HOMOSERINE LACTONE (AHL) BY EXPRESSION OF VIRULENCE GENE (HLYA) AND GENE RESPONSIBLE FOR BIOFILM (SRTA)

Author

Sanika Naik-Samant, Veda Manerikar, Milind Mohan Naik, and Komal Salkar

Subjects
Chemistry, Biofilm, Listeriolysin O, Homoserine, food and beverages, Virulence, biochemical phenomena, metabolism, and nutrition, Listeria infection, medicine.disease, medicine.disease_cause, Microbiology, Quorum sensing, chemistry.chemical_compound, Listeria monocytogenes, Sortase, medicine, Molecular Biology, Food Science, Biotechnology
Abstract

Listeria monocytogenes, a foodborne pathogen and well known causative agent of listeriosis in humans that is also known to form biofilm on utensils used in the dairy industry is well studied for peptide-based Quorum sensing system (agr QS system). In the present study, we are reporting L. monocytogenes strain BN3 to respond to an acyl-homoserine lactone (AHL) by expression of virulence gene (hlyA) and the gene responsible for biofilm formation (srtA) using Real-Time PCR technology. It revealed that in L. monocytogenes, hlyA gene (encodes listeriolysin O) expression increased 0.7-fold in response to 500 nM AHL (C4-HSL treated) as compared to control. Also, in response to 500 nM AHL, (C4-HSL treated) strain BN3 showed 27-fold up-regulation of srtA gene (encodes enzyme sortase) as compared to control. Our study confirmed the cross-talk between Gram-positive and Gram-negative bacteria since L. monocytogenes strain BN3 responds to C4-HSL, which is normally produced by Gram-negative bacteria. Here, we suggest targeting both agr based QS and AHL based QS system together in L. monocytogenes to tackle biofilm formed on milk cans in dairy industries and when treating listeria infections.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results