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Your search keyword '"Kai Borcherding"' showing total 3 results
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3 results on '"Kai Borcherding"'

1. Chemical characterization of the adhesive secretions of the salamander Plethodon shermani (Caudata, Plethodontidae)

Author

Janek von Byern, Ingo Grunwald, Max Kosok, Ralph A. Saporito, Ursula Dicke, Oliver Wetjen, Karsten Thiel, Kai Borcherding, Thomas Kowalik, and Martina Marchetti-Deschmann

Subjects
Medicine, Science
Abstract

Abstract Salamanders have developed a wide variety of antipredator mechanisms, including tail autotomy, colour patterns, and noxious skin secretions. As an addition to these tactics, the red-legged salamander (Plethodon shermani) uses adhesive secretions as part of its defensive strategy. The high bonding strength, the fast-curing nature, and the composition of the biobased materials makes salamander adhesives interesting for practical applications in the medical sector. To understand the adhesive secretions of P. shermani, its components were chemically analysed by energy dispersive X-ray spectroscopy (EDX), inductively coupled plasma mass spectrometry (ICP-MS), amino acid analysis, and spectroscopy (ATR-IR, Raman). In addition, proteins were separated by gel-electrophoresis and selected spots were characterised by peptide mass fingerprinting. The salamander secretion contains a high amount of water and predominantly proteins (around 77% in the dry stage). The gel-electrophoresis and peptide mass fingerprint analyses revealed a de novo set of peptides/proteins, largely with a pI between 5.0 and 8.0 and a molecular mass distribution between 10 and 170 kDa. Only low homologies with other proteins present in known databases could be identified. The results indicate that the secretions of the salamander Plethodon clearly differ chemically from those shown for other glue-producing terrestrial or marine species and thus represent a unique glue system.

Published
2017
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2. The rationale behind implant coatings to promote osteointegration, bone healing or regeneration

Author

Kai Borcherding, Gerhard Schmidmaier, Britt Wildemann, and Gunther O. Hofmann

Subjects
030222 orthopedics, medicine.medical_specialty, Implant surface, Bone Regeneration, business.industry, medicine.medical_treatment, Regeneration (biology), Dentistry, 030208 emergency & critical care medicine, Bone healing, Prostheses and Implants, Arthroplasty, Osseointegration, 03 medical and health sciences, 0302 clinical medicine, Osteogenesis, Orthopedic surgery, medicine, General Earth and Planetary Sciences, Humans, Implant, business, General Environmental Science
Abstract

Implant loosening, bone healing failure, implant-associated infections, and large bony defects remain challenges in orthopedic surgery. Implant surface modifications and coatings are being developed to promote osteointegration, prevent colonization by bacteria, and release bioactive factors. The following mini-review briefly discusses the clinical problem, explains the four "osteos", presents examples of coatings used for different orthopedic indications, and finally raises awareness of the coating and translational requirements.

Published
2020

3. Burst Release of Antibiotics Combined with Long-Term Release of Silver Targeting Implant-Associated Infections: Design, Characterization and in vitro Evaluation of Novel Implant Hybrid Surface

Author

Dirk Salz, Britt Wildemann, Uwe Specht, Ingo Grunwald, Karsten Thiel, Kai Borcherding, Nicole Bormann, Dennis Marx, and Linda Gätjen

Subjects
Biocompatibility, implant-associated infection, 02 engineering and technology, gentamicin, release, lcsh:Technology, Article, Osseointegration, Agar plate, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, silver, General Materials Science, titanium, Agar diffusion test, lcsh:Microscopy, lcsh:QC120-168.85, 030222 orthopedics, lcsh:QH201-278.5, lcsh:T, Chemistry, osseointegration, 021001 nanoscience & nanotechnology, Surface coating, lcsh:TA1-2040, surface coating, orthopedics, lcsh:Descriptive and experimental mechanics, Gentamicin, lcsh:Electrical engineering. Electronics. Nuclear engineering, Implant, lcsh:Engineering (General). Civil engineering (General), 0210 nano-technology, lcsh:TK1-9971, medicine.drug, Biomedical engineering
Abstract

Implant-associated infections represent a serious risk in human medicine and can lead to complications, revisions and in worst cases, amputations. To target these risks, the objective was to design a hybrid implant surface that allows a local burst release of antibiotics combined with long-term antimicrobial activity based on silver. The efficacy should be generated with simultaneous in vitro cytocompatibility. The investigations were performed on titanium K-wires and plates and gentamicin was selected as an illustrative antibiotic. A gentamicin depot (max 553 &micro, g/cm2) was created on the surface using laser structuring. The antibiotic was released within 15 min in phosphate buffered saline (PBS) or agar medium. Metallic silver particles (4 &micro, g/cm2) in a titanium dioxide layer were deposited using plasma vapor deposition (PVD). About 16% of the silver was released within 28 days in the agar medium. The local efficacy of the incorporated silver was demonstrated in a direct contact assay with a reduction of more than 99.99% (Escherichia coli). The local efficacy of the hybrid surface was confirmed in a zone of inhibition (ZOI) assay using Staphylococcus cohnii. The biocompatibility of the hybrid surface was proven using fibroblasts and osteoblasts as cell systems. The hybrid surface design seems to be promising as treatment of implant-associated infections, considering the achieved amount and release behavior of the active ingredients (gentamicin, silver). The generated in vitro results (efficacy, biocompatibility) proofed the concept. Further in vivo studies will be necessary translate the hybrid surface towards clinical applied research.

Published
2019
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