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Background Despite a large body of data suggesting that delivery of fertility care to cancer patients is inconsistent and frequently insufficient, there is a paucity of literature examining training in fertility preservation for those physicians expected to discuss options or execute therapy. The study objective was to compare fertility preservation training between Reproductive Endocrinology & Infertility (REI) and Gynecologic Oncology (GYN ONC) fellows and assess the need for additional education in this field. Methods A 38-item survey was administered to REI and GYN ONC fellows in the United states in April 2014. Survey items included: 1) Clinical exposure, perceived quality of training, and self-reported knowledge in fertility preservation; 2) an educational needs assessment of desire for additional training in fertility preservation. Results Seventy-nine responses were received from 137 REI and 160 GYN ONC fellows (response rate 27%). REI fellows reported seeing significantly more fertility preservation patients and rated their training more favorably than GYN ONC fellows (48% of REI fellows versus 7% of GYN ONC fellows rated training as ‘excellent’, p

A protein A immunoreactor (immobilized protein A) incorporating flow injection technique was used for on-line fluoroimmunoassay of human transferrin. Antibody immobilized on protein A and antibody-antigen complex formation took place in phosphate buffered saline (PBS, PH 7.4). After washing off excess lucifer yellow VS labelled human transferrin, the antibody-antigen complex was eluted with acid buffer and detected. Experimental variables have been studied and the method has been used to determine the transferrin contents in human serum.

There is an increase in the number of cases of syphilis in pregnancy in the United States. Fetal death may occur in syphilis from acute or chronic infections. A case is presented in which an acute fetal infection occurred. The patient presented at 31 weeks' gestation, with a decrease in fetal movements and non-reactive cardiotocography. Ultrasound and Doppler analysis of the fetal heart, cerebral and umbilical arteries, aorta and umbilical vein led to the suspicion of acute cardiac failure. An amniocentesis yielded a white cell count of 1122 white blood cells, with 91% polymorphs, but the Gram stain was negative. The fetus developed a persistent bradycardia and was delivered. The diagnosis of acute severe syphilitic funisitis was suspected from histological sections of the cord. Diagnosis was established from maternal and fetal blood. Modern ultrasound techniques, including imaging, Doppler and cardiotocography, can lead to the analysis of the pathophysiology of disease states. An acute syphilitic infection should be suspected when this constellation of findings is found. Copyright © 1991 International Society of Ultrasound in Obstetrics and Gynecology

Techniques using antibodies as reagents provide the sensitivity and selectivity demanded by modern clinical and environmental analyses. The use of non-isotopic labelling methods allows non-hazardous and homogeneous analysis formats, and offers new opportunities for multi-analyte assays and simple sensors. Some recent optical immunoassays illustrate the potential of such methods. Likely future developments in this area are surveyed.

Serum samples obtained from 59 persons who had acute necrotizing ulcerative gingivitis, periodontitis, syphilis, or Lyme disease were tested against Treponema phagedenis biotype Reiter, Treponema denticola, Treponema vincentii, and Treponema scoliodontum by indirect fluorescent-antibody staining methods. Although there were positive reactions for sera representing each of these study groups and for 20 (13%) of 156 samples collected from the general population (premarital screening for syphilis), titration endpoints were relatively low (less than or equal to 1:256). Serum samples from 18 persons who had gingivitis or periodontitis but no history of Lyme borreliosis were tested by enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi. Of these, five (28%) had immunoglobulin M antibody and four (22%) contained immunoglobulin G antibodies to this spirochete. Adsorption with either sorbent commercially prepared from T. phagedenis biotype Reiter or with washed, whole cells of T. phagedenis biotype Reiter reduced cross-reactivity in the enzyme-linked immunosorbent assay for Lyme borreliosis.

[1] Land surface processes are strongly controlled by vegetation cover. Current land surface models represent vegetation as a combination of leaf area index (LAI) and green vegetation fraction (GVF) parameters. The purpose of the study is to examine the impact of a spatially and temporally detailed Moderate Resolution Imaging Spectroradiometer (MODIS)-based GVF on surface processes in the NCEP Noah land surface model. The largest differences between the GVF data set currently used by the Noah model and the new MODIS GVF data set occur in winter and for tree-dominated vegetation classes. The greatest impact of the new GVF data on the surface energy and water balance is seen during the summer, when the transpiration is increased by more than 10 W/m 2 on average for most vegetation types and the July averaged daily transpiration rate is increased by up to 50 W/m 2 for evergreen needleleaf sites.

The sections in this article are 1 Recombination and Emission Line Shape 2 Efficiency and Brightness of LEDs 3 Human Eye Sensitivity 4 Light-Escape Problem 5 Led Structures and Fabrication 6 Novel Device Concepts

1. Bei der Koagulation des „Algor“-Sols wird O2 entwickelt. 2. Der Gehalt des bei den Versuchen entwickelten Sauerstoffes steht in einem direkten Verhaltnis zu der Konzentration der vorhandenen Kolloide. 3. Der Gehalt des gefundenen Sauerstoffes steht in einem chemisch aquivalenten Verhaltnis zu dem adsorbierten Barium. 4. Es wurde eine Theorie entwickelt, um die Bildung dieses Sauerstoffes in dem System zu erklaren. 5. Die Zunahme der Saure bei der Ausflockung des Kolloides steht in einem chemisch aquivalenten Verhaltnis zu der adsorbierten Bariummenge. 6. Die Technik zum Studium von Gasen, die bei der Ausflockung von Kolloiden durch einen Elektrolyten entwickelt werden, wird beschrieben.

Treponema pallidum-immobilizing (TPI) antibodies were examined in intradermally infected inbred strain 13 and adoptively immune inbred strain 2 guinea pigs. Both strains of animals produced TPI antibodies at or after 90 days of infection. TPI antibodies were not associated with the protective mechanism(s) operative after challenge in adoptively immune animals.

Spirochetes were isolated from the feces of 11 homosexual males who had diarrhea. The anaerobic organisms were isolated from a selective medium that consisted of Trypticase soy agar supplemented with either 5% horse or human blood, 400 micrograms of spectinomycin per ml, and 5 micrograms of polymyxin B per ml. Nonselective media that permitted good growth of these fastidious organisms were developed, and selected biochemical tests were performed. The tests included carbohydrate utilization, detection of certain enzymes, and determination of volatile fatty-acid end products of metabolism. Two growth patterns were noted on solid media, a haze of growth and production of small colonies. Based on the results of biochemical tests, patterns of preformed enzymes, and volatile fatty-acid production, we believe that the 11 isolates represent a heterogeneous group of spirochetes. The data suggest that the human colon may harbor unique strains of cultivable spirochetes; additional study of the taxonomy of the organisms and assessment of their virulence for humans are needed.