Author: "Ivan Rodriguez" / Topic: medicine - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Ivan Rodriguez"' showing total 85 results

Start Over Author "Ivan Rodriguez" Topic medicine

85 results on '"Ivan Rodriguez"'

1. Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets

Author: Kelsie M Rodriguez, Sara C Buch-Larsen, Ilsa T Kirby, Ivan Rodriguez Siordia, David Hutin, Marit Rasmussen, Denis M Grant, Larry L David, Jason Matthews, Michael L Nielsen, and Michael S Cohen
Subjects: ADP-ribosylation, PARPs, click chemistry, chemical genetics, proteomics, proximity labeling, Medicine, Science, Biology (General), QH301-705.5
Abstract: Poly(ADP-ribose) polymerase 7 (PARP-7) has emerged as a critically important member of a large enzyme family that catalyzes ADP-ribosylation in mammalian cells. PARP-7 is a critical regulator of the innate immune response. What remains unclear is the mechanism by which PARP-7 regulates this process, namely because the protein targets of PARP-7 mono-ADP-ribosylation (MARylation) are largely unknown. Here, we combine chemical genetics, proximity labeling, and proteome-wide amino acid ADP-ribosylation site profiling for identifying the direct targets and sites of PARP-7-mediated MARylation in a cellular context. We found that the inactive PARP family member, PARP-13—a critical regulator of the antiviral innate immune response—is a major target of PARP-7. PARP-13 is preferentially MARylated on cysteine residues in its RNA binding zinc finger domain. Proteome-wide ADP-ribosylation analysis reveals cysteine as a major MARylation acceptor of PARP-7. This study provides insight into PARP-7 targeting and MARylation site preference.
Published: 2021
Full Text: View/download PDF

2. Nod2 and Nod2-regulated microbiota protect BALB/c mice from diet-induced obesity and metabolic dysfunction

Author: Ivan Rodriguez-Nunez, Tiffany Caluag, Kori Kirby, Charles N. Rudick, Roman Dziarski, and Dipika Gupta
Subjects: Medicine, Science
Abstract: Abstract Genetics plays a central role in susceptibility to obesity and metabolic diseases. BALB/c mice are known to be resistant to high fat diet (HFD)-induced obesity, however the genetic cause remains unknown. We report that deletion of the innate immunity antibacterial gene Nod2 abolishes this resistance, as Nod2 −/− BALB/c mice developed HFD-dependent obesity and hallmark features of metabolic syndrome. Nod2 −/− HFD mice developed hyperlipidemia, hyperglycemia, glucose intolerance, increased adiposity, and steatosis, with large lipid droplets in their hepatocytes. These changes were accompanied by increased expression of immune genes in adipose tissue and differential expression of genes for lipid metabolism, signaling, stress, transport, cell cycle, and development in both adipose tissue and liver. Nod2 −/− HFD mice exhibited changes in the composition of the gut microbiota and long-term treatment with antibiotics abolished diet-dependent weight gain in Nod2 −/− mice, but not in wild type mice. Furthermore, microbiota from Nod2 −/− HFD mice transferred sensitivity to weight gain, steatosis, and hyperglycemia to wild type germ free mice. In summary, we have identified a novel role for Nod2 in obesity and demonstrate that Nod2 and Nod2-regulated microbiota protect BALB/c mice from diet-induced obesity and metabolic dysfunction.
Published: 2017
Full Text: View/download PDF

3. Long term functional plasticity of sensory inputs mediated by olfactory learning

Author: Nixon M Abraham, Roberto Vincis, Samuel Lagier, Ivan Rodriguez, and Alan Carleton
Subjects: sensory perception, imaging, behavior, Medicine, Science, Biology (General), QH301-705.5
Abstract: Sensory inputs are remarkably organized along all sensory pathways. While sensory representations are known to undergo plasticity at the higher levels of sensory pathways following peripheral lesions or sensory experience, less is known about the functional plasticity of peripheral inputs induced by learning. We addressed this question in the adult mouse olfactory system by combining odor discrimination studies with functional imaging of sensory input activity in awake mice. Here we show that associative learning, but not passive odor exposure, potentiates the strength of sensory inputs up to several weeks after the end of training. We conclude that experience-dependent plasticity can occur in the periphery of adult mouse olfactory system, which should improve odor detection and contribute towards accurate and fast odor discriminations.
Published: 2014
Full Text: View/download PDF

4. Divergent evolution among teleost V1r receptor genes.

Author: Patrick Pfister, Jerome Randall, Juan I Montoya-Burgos, and Ivan Rodriguez
Subjects: Medicine, Science
Abstract: The survival of vertebrate species is dependent on the ability of individuals to adequately interact with each other, a function often mediated by the olfactory system. Diverse olfactory receptor repertoires are used by this system to recognize chemicals. Among these receptors, the V1rs, encoded by a very large gene family in most mammals, are able to detect pheromones. Teleosts, which also express V1r receptors, possess a very limited V1r repertoire. Here, taking advantage of the possibility to unequivocally identify V1r orthologs in teleosts, we analyzed the olfactory expression and evolutionary constraints of a pair of clustered fish V1r receptor genes, V1r1 and V1r2. Orthologs of the two genes were found in zebrafish, medaka, and threespine stickleback, but a single representative was observed in tetraodontidae species. Analysis of V1r1 and V1r2 sequences from 12 different euteleost species indicate different evolutionary rates between the two paralogous genes, leading to a highly conserved V1r2 gene and a V1r1 gene under more relaxed selective constraint. Moreover, positively-selected sites were detected in specific branches of the V1r1 clade. Our results suggest a conserved agonist specificity of the V1R2 receptor between euteleost species, its loss in the tetraodontidae lineage, and the acquisition of different chemosensory characteristics for the V1R1 receptor.
Published: 2007
Full Text: View/download PDF

5. Trends in Mortality Due to Stimulants Use in Adolescents and Young Adults

Author: Surbhi Tyagi, Tasneem Kousar, David Leszkowitz, Rabeet Tariq, Rafael Abreu, Adam M. Qazi, Syeda W. Salari, Namrata Walia, Jessica O. Lat, Mahvish Renzu, Amina Jafar, and Ivan Rodriguez
Subjects: business.industry, Medicine, General Medicine, Young adult, business, Demography
Published: 2021

6. Cultural factors related to neuropsychological performance and brain atrophy among Hispanic older adults with amnestic Mild Cognitive Impairment (aMCI): A pilot study

Author: David A. Loewenstein, Ranjan Duara, Shanna L. Burke, Ivan Rodriguez, Amanda Orozco, Miriam J. Rodriguez, Lisandra Mendoza, and Monica Rosselli
Subjects: 050103 clinical psychology, media_common.quotation_subject, Pilot Projects, Neuropsychological Tests, Article, Literacy, Atrophy, Alzheimer Disease, Developmental and Educational Psychology, medicine, Humans, Mild neurocognitive disorder, Cognitive Dysfunction, 0501 psychology and cognitive sciences, Association (psychology), Cognitive impairment, Aged, media_common, medicine.diagnostic_test, 05 social sciences, Neuropsychology, Brain, Neurodegenerative Diseases, Magnetic resonance imaging, Hispanic or Latino, medicine.disease, Magnetic Resonance Imaging, Acculturation, Neuropsychology and Physiological Psychology, Psychology, Clinical psychology
Abstract: OBJECTIVES: This study examined the association of cultural factors and literacy to neuropsychological performance and measures of regional brain atrophy among Hispanic elders diagnosed with amnestic Mild Cognitive Impairment (aMCI). METHOD: Acculturation and literacy levels were measured among 45 subjects tested in Spanish; their primary language. Scores for measures of memory, executive functioning, and verbal fluency, as well as volumetric analysis of MRI scans of left hemisphere structures commonly affected by Alzheimer’s disease (AD) were examined. Linear regression models were employed to examine the association of acculturation and literacy to neuropsychological performance and MRI measures. RESULTS: After controlling for age, higher literacy levels were associated with better performance on phonemic verbal fluency (r = 0.300, p < .05), while higher levels of acculturation to the U.S. was associated with poorer performance on category verbal fluency (r = 0.300, p < .05). There was a significant inverse relationship after controlling for age between literacy and the left entorhinal cortex (r = −0.455, p < .05), left precuneus (r = −0.457, p < .05), and left posterior cingulate (r = −0.415, p < .05). CONCLUSIONS: Results of the current pilot study indicate that high acculturation to the U.S. among aMCI immigrants from Latin-American countries may hinder performance on verbal learning measures when they are administered in one’s primary language. Moreover, in this cohort, a higher literacy level, which is indicative of greater cognitive reserve, was associated with better performance in language measures, but with greater atrophy in brain regions susceptible to neurodegenerative disease. These preliminary findings should be further examined among larger cohorts and using more diverse measures, which capture other cultural constructs.
Published: 2020

7. Effects of exercise training on smoking-induced cardiopulmonary diseases. A review of the physiological mechanisms

Author: Ruy R. Campos, Ivan Rodriguez Núñez, Ximena Navarro, Carlos Manterola, Marcelo González, and Fernando Romero
Subjects: Gynecology, 03 medical and health sciences, medicine.medical_specialty, 0302 clinical medicine, 030228 respiratory system, business.industry, Physiology (medical), medicine, Orthopedics and Sports Medicine, 030212 general & internal medicine, business, Cardiopulmonary disease
Abstract: espanolObjetivo: El tabaquismo desencadena diversos mecanismos biologicos que producen El desarrollo de enfermedades cardiovasculares y pulmonares. Algunos estudios preclinicos han demostrado que el entrenamiento fisico puede ser eficaz en la reduccion del estres oxidativo y de la respuesta inflamatoria inducida por el tabaquismo. Por tanto, nuestro objetivo ha sido analizar el efecto del entrenamiento fisico en los sistemas pulmonar y cardiovascular en modelos experimentales de tabaquismo. Metodo: Se realizo una busqueda sistematica para localizar estudios orientados al analisis de los efectos del entrenamiento fisico sobre los danos pulmonares y/o cardiovasculares inducidos por el tabaquismo en modelos animales. Resultados: Se identificaron catorce estudios, todos realizados en ratas o ratones. Correr o nadar fueron los unicos metodos de entrenamiento y la exposicion de cuerpo entero al humo del tabaco fue el protocolo de tabaquismo mas frecuentemente usado en los estudios. Conclusion: Los estudios sustentan la hipotesis de que el entrenamiento fisico realizado antes, durante o despues del tabaquismo, puede reducir o incluso revertir, el estres oxidativo y la respuesta inflamatoria en animales expuestos al humo del tabaco, lo que podria contribuir en la recuperacion de la funcion cardiovascular y respiratoria. EnglishObjective: Cigarette smoking triggers a plethora of biological mechanisms that promote the development of cardiovascular and respiratory diseases. Some preclinical studies have shown that exercise training could be effective in blunting oxidative stress and inflammatory response induced by cigarette smoking. Therefore, we aim to analyze the effect of exercise training on pulmonary and cardiovascular system in experimental models of cigarette smoking. Methods: A systematic search was performed in order to identify studies addressed to evaluate the effects of exercise training on pulmonary and/or cardiovascular damage induced by cigarette smoking in animal models. Results: fourteen articles were identified, all of them performed in rats or mice. Running and swimming were the only training methods and whole-body smoke exposition was the most prevalent smoking protocol used in the studies. Conclusion: The studies support the hypothesis that exercise training performed before, concurrently or after smoking can blunt or even revert the oxidative stress and inflammatory response in animals exposed to cigarette smoke, which could contribute to recovering its cardiovascular and respiratory function. portuguesObjetivo: O tabagismo desencadeia uma diversidade de mecanismos biologicos que promovem o desenvolvimento de doencas cardiovasculares e respiratorias. Alguns estudos pre-clinicos demonstraram que o treinamento fisico (TE) pode ser eficaz na reducao do estresse oxidativo e da resposta inflamatoria induzida pelo tabagismo. Portanto, objetivamos analisar o efeito do treinamento fisico nos sistemas pulmonar e cardiovascular em modelos experimentais de tabagismo. Metodos: Uma busca sistematica foi realizada para identificar estudos direcionados para avaliar os efeitos do treinamento fisico sobre os danos pulmonares e / ou cardiovasculares induzidos pelo tabagismo em modelos animais. Resultados: Quatorze artigos foram identificados, todos realizados em ratos ou camundongos. Correr e nadar foram os unicos metodos de treinamento e a exposicao a fumaca de corpo inteiro foi o protocolo de tabagismo mais prevalente usado nos estudos. Conclusao: Os estudos sustentam a hipotese de que o treinamento fisico realizado antes, concomitantemente ou apos o tabagismo pode reduzir ou ate reverter o estresse oxidativo e a resposta inflamatoria em animais expostos a fumaca do cigarro, o que poderia contribuir para a recuperacao de sua funcao cardiovascular e respiratoria.
Published: 2019

8. Transcriptional adaptation of sensory neurons to GPCR identity and activity

Author: Leon Fodoulian, Alan Carleton, Luis Flores Horgue, Leonardo Marconi Archinto, Ivan Rodriguez, Madlaina Boillat, Joël Tuberosa, Alexis Assens, and Alexander Haider
Subjects: Olfactory system, Sensory Adaptation, Olfactory receptor, Sensory system, Biology, Sensory neuron, Adenylyl cyclase, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, medicine, Adaptation, Neuroscience, G protein-coupled receptor
Abstract: Sensory adaptation is critical to extract information from a changing world. Taking advantage of the extensive parallel coding lines present in the olfactory system, we explored the potential variations of neuronal identities before and after olfactory experience. We found that at rest, the transcriptomic profiles of olfactory sensory neuron populations are already highly divergent, specific to the olfactory receptor they express, and are surprisingly associated with the sequence of these latter. These divergent profiles further evolve in response to the environment, as odorant exposure leads to massive reprogramming via the modulation of transcription. Adenylyl cyclase 3, but not other main elements of the olfactory transduction cascade, plays a critical role in this activity-induced transcriptional adaptation. These findings highlight a broad range of sensory neuron identities that are present at rest and that adapt to the experience of the individual, thus providing a novel layer of complexity to sensory coding.
Published: 2021

9. Spred1 deficit promotes treatment resistance and transformation of chronic phase CML

Author: Le Xuan Truong Nguyen, Akihiko Yoshimura, Haris Ali, Paul Koller, Danilo Perrotti, Yasmin Elhajmoussa, Junjing Qiao, Ivan Rodriguez, Lucy Ghoda, Fang Chen, Chen Liang, Anthony S. Stein, Bin Amber Zhang, Mhairi Copland, Shanshan Suo, Dandan Zhao, Guido Marcucci, Anjia Han, Francesca Pellicano, and Dinh Hoa Hoang
Subjects: MAPK/ERK pathway, Cancer Research, Biology, Article, Mice, hemic and lymphatic diseases, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, medicine, Animals, Humans, Protein Kinase Inhibitors, Adaptor Proteins, Signal Transducing, Mice, Knockout, ABL, Gene Expression Regulation, Leukemic, breakpoint cluster region, Hematology, medicine.disease, Hematopoietic Stem Cells, Mice, Inbred C57BL, Haematopoiesis, medicine.anatomical_structure, Cell Transformation, Neoplastic, Oncology, Drug Resistance, Neoplasm, Cancer research, Neoplastic Stem Cells, Bone marrow, Stem cell, Homeostasis, Chronic myelogenous leukemia
Abstract: Spred1 is highly expressed in normal hematopoietic stem cells (HSCs). Lack of Spred1 function has been associated with aberrant hematopoiesis and acute leukemias. In chronic myelogenous leukemia (CML), Spred1 is reduced in patients with accelerated phase (AP) or blast crisis (BC) CML, thereby suggesting that deficit of this protein may contribute to disease transformation. In fact, Spred1 knockout (KO) in SCLtTA/BCR-ABL CML mice either globally, or restricted to hematopoietic cells (i.e., HSCs) or to endothelial cells (ECs), led to transformation of chronic phase (CP) CML into AP/BC CML. Upon BCR-ABL induction, all three Spred1 KO CML models showed AP/BC features. However, compared with global Spred1 KO, the AP/BC phenotypes of HSC-Spred1 KO and EC-Spred1 KO CML models were attenuated, suggesting a concurrent contribution of Spred1 deficit in multiple compartments of the leukemic bone marrow niche to the CML transformation. Spred1 KO, regardless if occurred in HSCs or in ECs, increased miR-126 in LSKs (Lin−Sca-1+c-Kit+), a population enriched in leukemic stem cells (LSCs), resulting in expansion of LSCs, likely through hyperactivation of the MAPK/ERK pathway that augmented Bcl-2 expression and stability. This ultimately led to enhancement of Bcl-2-dependent oxidative phosphorylation that supported homeostasis, survival and activity of LSCs and drove AP/BC transformation.
Published: 2021

10. Comparing the clinical applicability of wavefront phase imaging in keratoconus versus normal eyes

Author: Carolina Belda-Para, Gonzalo Velarde-Rodríguez, Miriam Velasco-Ocaña, Juan M. Trujillo-Sevilla, Iván Rodríguez-Méndez, Javier Rodríguez-Martin, Nicolas Alejandre-Alba, Silvia Rodríguez-García, and José M. Rodríguez-Ramos
Subjects: Medicine, Science
Abstract: Abstract The aim of this work is to quantitatively assess the wavefront phase of keratoconic eyes measured by the ocular aberrometer t·eyede (based on WaveFront Phase Imaging Sensor), characterized by a lateral resolution of 8.6 µm without requiring any optical element to sample the wavefront information. We evaluated the parameters: root mean square error, Peak-to-Valley, and amplitude of the predominant frequency (Fourier Transform analysis) of a section of the High-Pass filter map in keratoconic and healthy cohorts. Furthermore, we have analyzed keratoconic eyes that presented dark–light bands in this map to assess their period and orientation with the Fourier Transform. There are significant statistical differences (p value
Published: 2024
Full Text: View/download PDF

11. Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Author: Labeau, Sonia O, Afonso, Elsa, Benbenishty, Julie, Blackwood, Bronagh, Boulanger, Carole, Brett, Stephen J, Calvino-Gunther, Silvia, Chaboyer, Wendy, Coyer, Fiona, Deschepper, Mieke, François, Guy, Honore, Patrick M, Jankovic, Radmilo, Khanna, Ashish K, Llaurado-Serra, Mireia, Lin, Frances, Rose, Louise, Rubulotta, Francesca, Saager, Leif, Williams, Ged, Blot, Stijn I, Dritan, Muzha, Antoni Margarit Ribas, Fernando, Lipovesty, Cecilia, Loudet, Fiona, Coyer, Philipp, Eller, Nafseen, Mostafa, Patrick, M Honoré, Vanesa Mercado Telleria, Jasmina, Smajic, Paula Cristina Nogueira, Khalid Mahmood Khan Nafees, Romuald, Hentchoya, Louise, Rose, Javiera, Soledad, Frances, Lin, Yenny, Cardenas, Amylkar Garay Reyes, Alan, Sustic, Meropi, Mpouzika, Tamas, Vymazal, Hanne Irene Jensen, Hernan, Aguirre-Bermeo, Liivi, Maddison, Maija, Valta, Silvia, Calvino-Gunther, Frank, Bloos, Faustina Excel Adipa, Vasilios, Koulouras, Judy, Enamorado, Zsuzsann, Ágoston, Hrönn, Birgisdóttir, Amit, Gupta, Mohan, Gurjar, Bram, Kilapong, Seyed Mohammadreza Hashemian, Ignacio, Martin-Loeches, Julie, Benbenishty, Andrea, Cortegiani, Kelly, Fletcher, Yoshiro, Hayashi, Wangari, Waweru-Siika, Khalid, Abidi, Sang-Min, Lee, Burhan, Hadri, Mihails, Dolgusevs, Fayez François Abillama, Tomas, Jovaisa, Cyril, Thix, Muhammed, Elhadi, Basri Mat Nor, Shanti, Ratnam, Mohd Zulfakar Mazlan, Sundaresan, Maiyalagan, Luis, Sánchez-Hurtado, Adrian, Belii, Mendsaikhan, Naranpurev, Prabha, Gautam, Dylan De Lange, Rachael, Parke, Rose Ekama Ilesanmi, Mirjana, Shosholcheva, Antonija, Petosic, Ranveig, Lind, Madiha Hashmi Ffarcsi, Javier, Bogarin, Aaron Mark Hernandez, Malgorzata, Mikaszewska-Sokolewicz, Bruno, Sousa, Dana, Tomescu, Dorel, Sandesc, Theogene, Twagirumugabe, Vitaly, Gusarov, Maie, Ebaid, Radmilo, Jankovic, Gari, Slobodianiuk, Andrea, Martonova, Rihard, Knafelj, Mervyn, Mer, Emilio, Maseda, Bernardo, Panka, Joerg, C Schefold, Eva, Joelsson-Alm, Konlawij, Trongtrakul, Lorna, Merritt-Charles, Lamia Ouanes Besbes, Yalım, Dikmen, Lesia, Zgrzheblovska, Mark, Fielding, Francesca, Rubulotta, Ashish, K Khanna, Leif, Saager, Ingrid von der Osten, Alban, Greca, Alma, Cani, Nordian, Xhindi, Genci, Hyska, Antonio Margarit Ribas, Susana, Pinto, Paulo, Alves, Romina, Esposito, Emanuel, Valgolio, John Thomas Sanchez Minope, Antonio, Abdala, Maria, Ayala, Silvina, Bravo, Ana, Bantar, Patricia, Delgado, Gustavo, Badariotti, Fernando, Lipovestky, Ana, Diaz, Pablo, Saul, Mariano, Setten, Alejandra, Aucapina, Ysica, Acosta, Victor, Gonzalez, Luis, Camputaro, Fernando, Baccaro, Robert, Villa, Marcela, Mastantuono, Emiliano, Dean, Oscar Fernández Rostello, Patricia, Brizuela, Julio Ricardo Bartoli, Matias, Guereschi, Cristian, Quiroga, Sofia, Putruele, Paula, Villegas, Veronica, Curilen, Ruben, Fernandez, Mariangeles Gabriela Nocheretti, Rosana Gabriela Escalante, Cecilia Inés Loudet, Silvia, Fernandez, Ana Laura Gonzalez, Gustavo Andres Alvarez, Federico, Iglesias, Silvia, Chaparro, Graciela, Zakalik, Gonzalo, Pagella, Matías, Baini, Pierina Arias Campos, Ignacio, Sabbag, Armando, Schmukler, Imelda Perdomo Fonseca, Gonzalo Martín Alvarez, Mario, Ramirez, Fernando, Tapia, Carlos Alejandro Bascary, Graciela Del Valle Gimenez, Fernando Pablo Bertoletti, Esteban, Milioto, Pablo Julio Maldonaldo Bonsignore, Maria Alejandra Fernandez, Julie, Smith, Tim, Chimunda, Lorraine, Thompson, Teena, Maguire, Wendy, Chaboyer, Stacey, Watts, Marion, Mitchell, Madeleine, Powell, India, Lye, Leanne, Parsons, Nerilee, Baker, Claire, Reynolds, Amy, Thompson, Kristy, Masters, Kellie, Sosnowski, Lynette, Morrison, Gavin, D Leslie, Ramanathan, Lakshmanan, Alexis, Tabah, Wendy, Brown, Sharon, McDowell-Skaines, Andrea, Mclucas, Chris, Smith, Mandy, Tallot, Sarah, Jones, Michelle, Barakat-Johnson, Thomas, Leong, Rand, Butcher, Kerrie, Martin, Philipp, Douschan, Dirk von Lewinski, René, Schmutz, Uta, Kolussi, Fatema, Salman, Zainab, Ateya, Koen De Decker, Niels Van Regenmortel, Anita, Jans, Patricia, Wijnands, Stefano, Coremans, Patrick, M Honore, David De Bels, Tanja, Depuydt, Caroline, Paillet, Luc-Marie, Jacquet, Walter, Swinnen, Francis, Hannes, Matthia, Mergeay, Stijn Van de Velde, Silvie, Allaert, Pieter, Hoste, Christophe, Borin, Sandrine, Balon, Vincent, Fraipont, Patrick, Biston, Nicolas De Schryver, Thierry, Dugernier, Ilse Van Cotthem, Angelica Olivetto de Almeida, Silvia Angelica Jorge, Delmiro, Becker, Raysa Cristina Schmidt, Evellyn, Oliveira, Aline, Ramalho, Eliane, Mazocoli, Audrey, Fioretti, Elaine, Barros, Leticia, Serpa, Suzana, Bianchini, Ticiane, Campanili, Taís, Pantaleao, Paulo Carlos Garcia, Ana Lucia Vitti Ronchini, Rayanne, Santos, Nurulhuda Binti, A Manap, Sean, Bagshaw, Dominic, Carney, Jon, Davidow, Ella, Rokosh, Andréa Maria Laizner, Samantha, Smith, Megan, Mcquirter, Betty Star Kampayana, René, Favre, Martin, Sills, Julie, Dallaire, Cathy, Becker, Sherissa, Microys, Bonnie, Bowes, Jennifer, Lajeunesse, Rishi, Ghosh, Jacqueline, Baptiste-Savoie, Rose, Raizman, Gabriel, Suen, Noushin, Taghavi, Orla, Smith, Clare, Fielding, Julieta, Canales, Pia, Molina, Javiera, Chaparro, Maria Idalia Sepulveda, Matias Jesús Flamm Zamorano, Pamela, Rocha, Ximena, Villanueva, Paola, Araya, Meneses, Dayan, Fernando, Avalos, Xiaohan, Li, Liu, Yu, Xinxia, Li, Xiaoyan, Chen, Zhixia, Jiang, Jing, Yang, Jingfang, Chen, Lei, Yang, Kefang, Wang, Jie, Gao, Xiuhua, Fang, Ronghua, Zhao, Xinhua, Xia, Hongmei, Liu, Jing, Li, Haiyan, Wang, Gen, Meng, Yanhong, Di, Damei, Wang, Rong Hua Zhao, Li Ping Hu, Peipei, Xu, Qing Feng Jiao, Hai Yun Wang, Chun Jie Xia, Yan, Liu, Mei, Ye, Yan, Wan, Wenmei, Wang, Yajun, Ding, Aiua, Ren, Yan, Gao, Qi, Li, Guifang, Du, Yanling, Shen, Yanming, Ding, Ning, Li, Cui, Yuan, Lei, Tan, Qiang, Lin, Hailing, Guo, Howe, Yan, Xiao, Xu, Wei, Zhang, Jinxian, Liang, Libing, Zhang, Eryun, Tian, Qian, Zhao, Lin, Insu, Jingwen, Dong, Yanmei, Gu, Ying, Liu, Lina, Zhao, Wei, Wang, Hongmei, Qiao, Lili, Tuo, Mengmeng, Lv, Jin Yu Zhu, Jifen, Zhu, Ying, Wei, Man, Liu, Yin, He, Jiyin, Cheng, Jin, Liu, Jia, Na, Dongfang, Wei, Qing, Li, Xiaoying, Wu, Huan, Duan, Dongliang, Lin, Qiong, Liang, Xiaofang, Luo, Yunfeng, Xiong, Rong Fen Huang, Jing, Fu, Tao, Zan, Man, Ye, Zeya, Shi, Yanfei, Long, Yang, Lei, Xiaodi, Liu, Chen, Yumei, Lingling, Wang, Yali, Zhang, Yan, Xu, Cheng, Wang, Zhijuan, Chengxia, Sun, Jinhui, Song, Yingli, Wang, Xiumei, Liu, Yupeng, Liu, Yuxia, Yuan, Qingping, Huang, Fengling, Yang, Yun, Wu, Xianping, Luo, Xiaowu, Bai, Hong, Zheng, Min, Song, Yue, Sun, Zhangshuangzi, Li, Feifei, Luo, Miao, Liu, Li Chuntang Li, Xinjian, Li, Guiping, Zhang, Lina, Xiao, Tingting, Yu, Guangyuan, Gao, Wei, Wei, Fanglan, Wang, Ting, Han, Tingting, Li, Zeng, Qi, Jing Mei Zeng, Yan, Long, Fuqun, Pan, Jing, Wang, Guoxue, He, Haiyan, Chen, Feifei, Zhang, Chao, Yu, Gao, Chunhua, Xiuying, Yao, Dongmei, Bai, Liu, Lu, Xuelian, Xu, Yan, Wang, Xuejuan, Liang, Zhang, Na, Aizhi, Zhang, Xiaochun, Hu, Hui, Zhang, Ruixia, Wang, Poon Shing Tak, Sung Wai Ho, Qun Xia Jiang, Xinran, Ding, Liu, Hong, Limei, Miao, Zhaoxia, Feng, Liping, Huang, Juan, Wu, Yuping, Wang, Jiye, Guo, Baoke, Zhang, Chaoqun, Ma, Han, Yu, Congcong, Liu, Min, Ding, Linlin, Luan, Jing, Zheng, Shanshan, Lv, Shumin, Jiang, Wenzhen, Cao, Xiujuan, Xue, Guangyan, Liu, Xiyan, Wei, Youru, Jiang, Zhiru, Yao, Gao, Li, Jinhua, Li, Wenwen, Zhao, Mei, Jiang, Junping, Hao, Jing, Zhang, Caiju, Song, Feifei, Chen, Shuhui, Wang, Lili, Hu, Deyan, Cao, Jianhong, Wan, Xiaomin, Wang, Hongyan, Shao, Zhenxia, Zhang, Xia, Cui, Jingyu, Liu, Lijuan, Zhao, Xingguo, Li, Limei, Fan, Ling, Zhang, Min, Yu, Biyan, Li, Chunxia, Li, Ling, Liu, Xuelian, Liu, Wenmin, Chen, Yan, Li, Zhang, Zhigang, Yuchen, Wu, Chenghau, Mu, Guoyan, Zhu, Fan, Yang, Qi, Bo, Ling, Li, Meili, Chen, Jing Hua Jiang, Hai, Yin, Xuelian, Pang, Yue Ying Gong, Shunzhu, Yang, Xiaoli, Yan, Xianhong, Zheng, Dehong, Lei, Lei, Lei, Yinhua, Guo, Lihong, Liu, Jing, Yu, Wei, Sun, Aiping, Bi, Weiwei, Li, Yang, Wu, Ji, Li, Dongshu, Ni, Zijing, Wu, Bing, Song, Qin, Fei, Yang, Xiaoyan, Qiong, Ran, Xixi, Li, Xueping, Jiao, Hua, Ji, Sun, Zhiping, Hong, Ma, Jianhong, Mu, Yanhua, Hao, Yin, Li, Ying, Wang, Caihong, Hui, Wenjie, Ju, Yuxia, Huo, Yuxia, Wang, Lei, Chen, Yan, Yan, Qingli, Zhao, Hongjuan, Chen, Guijun, Bao, Ying, Cao, Hong, Li, Hong, Zhang, Ying, Zhang, Lina, Xu, Jia, Guixiang, Ying, Li, Hui Min Zhao, Xia, Huang, Zhaoxing, Dai, Yanman, Jian, Hongsu, Zhang, Zhixia, Tian, Zu Qing Cao, Miao, Li, Yang, Liu, Fei, Ouyang, Fuying, Ma, Wangyan, Jin, Liuyan, Ge, Shifen, Wu, Weilian, Yuan, Tianfei, Chen, Guanxiu, Shi, Zhihong, Chen, Kewei, Liu, Xue, Lin, Yuemen, Ly, Sun, Lijuan, Xiao Fang Tian, Shuo, Wang, Zhangxia, Feng, Xiaozhe, Liu, Yunchun, Dong, Jundi, Zhang, Nie, Bocui, Guoxian, Wang, Yingjuan, Zhao, Xiaojun, Wu, Qiao, Yang, Rongjun Ling Hu, Xue Qin Li, Zhu Jun Yu, Yanlan, Yao, Xiaoqiong, Deng, Yan, Xiao, Yan, Xie, Yanping, Yang, Huai, Yang, Yuming, Zhou, Zhuqing, Li, Min, Xiao, Yongxia, Yang, Yani, Tian, Luz Marina Silva Gama, Juan Sebastian Hernandez, Nestor, Caicedo, Jorge, Marin, Maria-Elena, Ochoa, Monica, Gomez, José, Rojas-Suarez, Jeniffer, Gonzalez, Amylkar José Garay Reyes, Edwin, Chapeta, Estefania, Orozco, Ina, Filipović-Grčić, Anita, Vuković, Suzana, Pečenković, Aleksandar, Šuput, Gordana, Zivanovic-Posilovic, Armanda, Bozena, Nikolina, Udiljak, Morena, Milic, Renata Curic Radivojevic, Slobodan, Mihaljevic, Marijana, Matas, Dinko, Tonkovic, Hemena, Čuljak, Ivana, Herceg, Gordana, Pavlisa, Milena, Dobric, Tatjana, Beker, Višnja Nesek Adam, Tanja, Goranovic, Chrysanthos, Markoulias, Mina, Mathaios, Maria, Mylordou, Eleni, Achilleos, Pavlina, Kleanthous, Veronika, Kotanidi, Maria, Foka, Iwy, Charalabous, Anna, Alexandrou, Marios, Georgiou, Artemis, Patsalos, Sofia, Zepoy, Constantina, Constantinou, Petr, Piza, Tomas, Vymazal, Elisabeth, Wiborg, Louise, Bruhn, Karin, Kaasby, Karin Rehnholt Pedersen, Sanne, Mikkelsen, Marie, Collet, Anne, Langvad, Hanne, Andresen, Susanne, Fischer, Inger Ebbesen Kjærgård, Britta, Jepsen, Birthe, Husted, Morten, Bestle, Anne Marie Kodal, Tina Charlotte Bitsch Hansen, Anne Sofie Bomholt Pedersen, Tina Damgaar Thomsen, Anisette, Hoegenhaven, Mette, From, Tine Melgaard Frandsen, Grit, Henning, Anja, Hansen, Inger Abildgaard Bliksted, Luis Mario Tamayo, Pedro, Mogrovejo, Carolina, Palaez, Diego Rolando Morocho Tutillo, Cintia Valencia Hurtado, Maria Fernanda García, Diana, Alvarez, Fausto, Guerrero, Alexandra, Vasquez, Martin, Kütimets, Kadri, Tamme, Eneli, Anvelt, Lomangisi, Dlamini-Sserumaga, Carita, Löfqvist, Virpi, Lusenius, Outi, Kauppi, Jenni-Katarina, Sakki, Tarja, Tervo-Heikkinen, Ulla, Kesti, Merja, Merilainen, Elina, Karjula, Minna, Peltomaa, Auli, Palmu, Maarit, Ahtiala, Maija Anniina Valta, Hervé, Mentec, Gaëtan, Plantefève, Guillaume, Besch, Sébastien, Pili-Floury, Stanislas, Ledochowski, Marc Danguy des Déserts, Christophe, Giacardi, Cédric, Daubin, Audrey, Massard, Yann Le Guen, Agnès, Blanc, Simon, Mandaroux, Silvia Calvino Günther, Prune, Avogadro, Anthony, Radavidson, Jean, Turc, Sébastien, Jochmans, Hervé, Quintard, Laetitia, Boyer, Cédric, Bruel, François, Philippart, Philippe, Montravers, Enora, Atchade, Nadine, Flessel, Benoît, Chinardet, Léa, Soulisse, Cindy, Pillard, Delphine, Ngo, Benjamin, Bongiorno, Nathalie, Heitzler, Virginie, Souppart, Nathalie, Gautheret, Jean-Francois, Timsit, Fatiha, Essardy, Muriel, Fartoukh, Daisy, Mehay, Fabienne, Etourneau, Jean-Christophe, Farkas, Pascal, Beuret, Gabriel, Preda, Etienne De Montmollin, Vincent, Castelain, Ulrich, Jaschinski, Monika, Rothenfusser, Detlef, Kindgen-Milles, Thomas, Dimski, Christine, Fiedler, Tobias, Heinicke, Patrick, Meybohm, Tobias, Schulze, Marc, Bota, Sabrina, Pelz, Tobias, Odenthal, Martin, Christ, Kathrin, Bösl, Achilleas, Chovas, Sebastian, Stehr, Philipp, Simon, Sarah, Grotheer, Sebastian, Schüppel, Stefan, Schaller, Lea, Albrecht, Andeas, Stübner, Stephan, Graeser, Nina, Kolbe, Martina, Lausch, Anja, Diers, Ulf, Guenther, Reimer, Riessen, Martin, Roller, Irene Pearl Osei, Anita-Chrysolyte, Kusi-Appiah, Yakubu, H Yakubu, Belinda, Guadi-Gosh, Christos, Dragoumanis, Christos, Christofis, Nikolaos, Kazakos, Styliani, Bastani, Charalampos, Martinos, Vasileios, Bekos, Metaxia, Papanikolaou, Theonymfi, Papavasilopoulou, Anna, Efthymiou, Vasiliki, Chantziara, Anna, Kyriakoudi, Nikolaos, Kakaras, Chrisi, Diakaki, Aikaterini, Flevari, Charikleia, Nikolaou, Kounougeri, Katerina, Lamprini, Avramopoulou, Kyriaki, Tsikritsaki, Georgios, Gkiokas, Eirini, Pantiora, Chrysostomos, Katsenos, Eirini-Chysovalanto, Patsiou, Paraskevi, Alexandropoulou, Ioannis, Koutsodimitropoulos, Epaminontas, Farmakis, Konstantina, Nestora, Marinos, Chatzis, Eumorfia, Kondili, Stella, Soundoulounaki, Ourania, Mousafiri, Dimitra, Lepida, Antonia, Liarmakopoulou, Georgios, Papathanakos, Mrina, Oikonomou, Panagiotis, Ioannides, Dimitrios, Papadopoulos, Ioannis, Staikos, Maria, Stafylaraki, Bogdan, Raitsiou, Konstantinos, Mandis, Ifigenia, Ravani, Styliani, Kourelea, Aikaterini, Efthimiou, Giannoula, Thoma, Apostolos, Bakas, Konstantinos, Psarulis, Souzana, Anisoglou, Eirini, Papageorgiou, Evangelia, Michailidou, Thomai, Tholioti, Athena, Lavrentieva, Evdokia, Sourla, Anastasia, Spyropoulou, Nikolaos, Pantelas, Kristina Mariana Matei Stalika, Ioannis, Georgakas, Antigoni, Karathanou, Syragoula, Tsikriki, Aikaterini, Dimoula, Sofia, Kanakaki, Aristeidis, Vakalos, Konstantinos, Pagioulas, Judy Enamorado Enamorado, Gabor, Nardai, Fatime, Hawchar, Asbjorn, Blondal, Brynja, Rygvadottir, Rannveig, J Jonasdottir, Hrönn, Birgisdottir, Bhagyesh, Shah, Shuchi, Kaushik, Swagata, Tripathy, Mukta, Singh, Sonika, Agarwal, Manish, Gupta, Meraj, Ahmad, Kishore, Mangal, Vaibhav, Bhargava, Vilas, Kushare, Simant, Jha, Lakshay, Bhakhtiani, Manoj, Kamal, Arvind, Baronia, Ade, Susanti, Mayang Indah Lestari, Zulkifli, Zulkifli, Windu, Baskoro, Farid, Zand, Fatemeh, Zarei, Ata, Mahmoodpoor, Farshad, Heidari, Fateme, Jafaraghaee, Aidan, O'Shea, Fiona, O'Shea, Caroline, O'Donnell, Geraldine, Craig, Gerry, Fitzpatrick, Lisa, Dunne, Jennifer, Hastings, Brian, Marsh, Caitriona, Cody, Elizabeth, Campbell, Deirdre, Doyle, Michelle, Pacturanan, Christine, Sheehan, Annette, Carey, Charlotte, Carter, Regina, Mulvey, Damien O'Connell Rosemary Finn, Catherine, Motherway, Amy, Walsh, Jennifer, Kehoe, Shella, Delossantos, Jennifer, Lalor, Siobhan, O'Nuallain, Helena, Behan, Sandra, Mcpherson, Ailesh, Corcoran, Patricia, Gordon, Glenda, Rooney, Dassy, Levy, Mazal, Azencot, Vladimir, Gurevich, Alinoy, Lavy, Valentina, Bendelari, Romina, Marconi, Antonio, Barone, Chiara, Gatti, Andrea, Giampaoletti, Cinzia, Borgognoni, Davide Massimo Ghioldi, Arena, Raimondo, Giacomo, Castiglione, Anna Vita Bruno, Giorgia, Rubulotta, Antonella, Mo, Amalia, Corso, Salvatore, Girianni, Andrea, Bruni, Eugenio, Garofalo, Salvatore Maurizio Maggiore, Alessandro Di Risio, Italo, Calamai, Rosario, Spina, Savino, Spadaro, Carlo Alberto Volta, Antonella, Cotoia, Lucia, Mirabella, Laura, Maulicino, Giancarlo, Abregal, Maria, Donvito, Paolo, D'Ambrosio, Filipo, Binda, Ileana, Adamina, Alessandro, Galazzi, Alessandra, Negro, Rosanna, Vaschetto, Fabio, Capuzzi, Margherita, Boschetto, Lucia, Stivanello, Luciano, Bonaccorso, Chiara, Megna, Pasquale, Iozzo, Antonino, Rizzo, Giovanni, Scire, Maria Rosa Taibi, Francesca Paola Tranello, Antonio, Manzo, Lidia, Traina, Beatrice, Pastore, Attilio, Quaini, Gian Domenico Giusti, Gloria, Montaldi, Federica, Piergentili, Federica, Mancini, Simona, Casaioli, Francesco, Uccelli, Fabio, Guarracino, Adriana, Onelli, Valentina Di Gravio, Maria, Cossu, Oliva, Matrona, Monica, Rocco, Daniela, Alampi, Federica, Dellafiore, Flavia, Ranalli, Matteo, Bossolasco, Elisabetta, Brizio, Pina, Migliorino, Paolo, Cortellazi, Moris, Rosati, Francesco, D'Ambrosio, Catia, Quagliotto, Erik, Roman-Pognuz, Alberto, Peratoner, De Rosa, Silvia, Marina Alessandra Martin, Francesca De Sanctis, Paolo, Ciorba, Patrick, Toppin, Hyacinth, Harding-Goldson, Shunsuke, Taito, Nobuaki, Shime, Ryohey, Yamamoto, Fumiya, Kanda, Akemi, Hirao, Moritoki, Egi, Ayako, Noguchi, Satoru, Hashimoto, Umeda, Aya, Hideaki, Sakuramoto, Akira, Ohuchi, Jun, Kataoka, Kumi, Maruyama, Izumi, Nakayama, Yoshimasa, Nishime, Koji, Fujimoto, Kenji, Takahashi, Mayumi, Tsujimoto, Masako, Shimizu, Eunice, Tole, Malcolm, C Correia, Je Hyeong Kim, Sunghoon, Park, Kyung Chan Kim, Jonghyun, Baek, Jung-Min, Bae, So Young Park, Tai Sun Park, Heung Bum Lee, Seung Yong Park, Jisoo, Park, Lee, Yeon-Joo, Cho, Young-Jae, Sang-Miin, Lee, Kyeongman, Jeon, Seok Chan Kim, Jongmin, Lee, Hyun Keun Chee, Jin Won Huh, Yun Su Sim, Junghyun, Kim, Youjin, Chang, Jong-Joon, Ahn, Byung Ju Kang, Won-Yeon, Lee, Seok Jeong Lee, Nehat, Baftiu, Ivasr, Krastins, Sonia, Stiban, Michel El Feghaly, Elie, Gharios, Marie, Merheb, Mohamed, Benlamin, Ala, Khaled, Wesal Ali Belkhair, Majd, Tabib, Firas, Ashour, Ahmed, Elhadi, Osama Wanees Emhemid Tababa, Taha, Khaled, Soad Imhmed, R Alkhumsi, Abdualhamid, I Alshrif, Ahmed Ali Aboufray, Aya, Alabuzidi, Ahmed Ramadan Triki, Mala, Elgammudi, Hajer Ben Zahra, Enas, Soula, Maram Milud Said Al-Alawi, Hazem, Ahmed, Mohamed Abdurazzag Ali Ghula, Saulius, Vosylius, Lucie, Mouton, Touraj, Rastegar, Claude, Sertznig, Gilles, Martin, Christian, Theisen, Christian, Ferretti, Fränk, Gils, Marc, Gallion, Asmah, Zainudin, Laila Kamaliah Kamalul Bahrin, Shanti Rudra Deva, Azmin Huda Abdul Rahim, Sherliza, Wahab, W Nazaruddin, W Hassan, Wan Nasrudin Wan Ismail, Mohd Nazri Ali, Tien Meng Khoo, Noryani Mohd Samat, Jenny May Geok Tong, Nik Azman Nik Adib, Mohd Basri Mat Nor, Shanthi, Ratnam, Nahla, Ismail, Siti Rohayah Sulaiman, Kit Weng Foong, Anita, Alias, Ngu Pei Hua, Jorge Macias Zermeno, Daniel, Blanco, Karely, Duran, Claudia Lizbeth Lopez Nava, San Juan Roman Nandyelly, Luis Alejandro Sanchez-Hurtado, Brigitte, Tejeda-Huezo, Mario Del Moral Armengol, Luis Pedro Ambriz Nava, Jorge Guerra Herrera, Gilberto Felipe Vazquez de Anda, Humberto, Gallegos-Perez, Nancy, Hernandez-Sanchez, Lucia, Hernandez-Ponce, Luis, Gorordo-Delsol, Marcos, Hernandez-Romero, Saira, Gomez, Fernando, Molinar, Silvio, A Ñamendys-Silva, Juan, P Romero-Gonzalez, Daira, Gonzalez, Antonio, Landaverde, Miguel Ángel Sosa, Berenice, Navarro, José Ivan Rodriguez de Molina Serrano, Sergio Reyes Iburrigarro, Alejandro, Ibarra, Joaquin, Aguirre, Mayra, Martinez-Gonzalez, Nayeli Rocio Cañas Padilla, Ana Alícia Velarde Pineda, Missael Vladimir Espinoza Villafuerte, María Ocotlan Gonzalez Herrera, Battsetseg, Baasanjav, Abdelhamid, Hachimi, Mina, Elkhayari, Tarek, Dendane, Nisha Bhandari Subedi, Sabina Dhakal Pathak, Meena, Manandhar, Laura Van Gulik, Mark Van Den Brink, Peter Van Vliet, Benjamin, Gerretsen, Lettie Van Den Berg, Marina De Haan, Binny, Tuinstra, Paul, Kuijpers, Jennifer, Reijntjens, Jan Wytze Vermeijden, Martin, Rinket, Margijske, Vanroest, Auke, Reidinga, Bert, Loef, Willem, Dieperink, Marisa, Onrust, Tom, Dormans, Laura, Bormans, Matty, Koopmans, Rik, T Gerritsen, Arlette Van Den Elst, Mirjam, Evers, Oscar, Oiting, Rob, Wilting, Bart, Ramaker, Mark van der Kuil, Jan-Willem, Fijen, Lenneke, Haas, Jasper, Haringman, Lynette, Newby, Eileen, Gilder, Danielle, Hacking, Rica, Dagooc, Rima, Song, Hansjoerg, Waibel, Frances, Dawn, Jackie, Rapley, Llesley, Chadwick, Carmel, Chapman, Petra, Crone, Jonathan, Albrett, Peter, Marko, Jennifer, Goodson, Troy, Browne, Richard, Whitticase, Cheryl, Davidson, Harriet, Judd, Daniel, Owens, Tonia, Onyeka, Innocent, Ugwu, Rose, Ilesanmi, Prisca Olabisi Adejumo, Afolabi, Owojuyigbe, Anthony, Adenekan, Stella, Uba, Christiana, Chime, Deborah, Jibrin, Babangida John Sankey, Oyebola, Adekola, Simeon, Olanipekun, Mirjana, Shosolcheva, Vanja, Gievski, Andrijan, Kartalov, Filip, Naumovski, Biljana, Kuzmanovska, Angela, Trposak, Zaneta, Bogoevska-Miteva, Rodney, Rosalia, Brita Fosser Olsen, Britt, Sjobo, Karianne Dale Jensen, Drammen, Sykehus, Birgitte Fosser Johansen, Esben, Straede, Edda, Johansen, Inger Johanne Finnstrom, Annette, Toellefsen, Hege, Ostenjo, Hege, Bjorgen, Bjorn, Bratsberg, Elin, Kristoffersen, Elin Mari Skorstad, Siri, Hansen, Sylvi, Vullum, Gro Anne Lunde, Wenche, Arntsen, Mette, Lund, Gro Ringstad Akselsen, Kristina Reinertsen Monstad, Ane, Stenset, Hanne, Haugom, Bjoern, Monsen, Lisa, Høgvall, Siw, Trudvang, Britt, Galaaen, Siv Karin Malmin, Marit Hildegunn Andersen, Rita Foss Hargott, Yvonne, Andersen, Elin, Steffenak, Marit, Nyhus, Barbro, Meland, Madiha, Hashmi, Noelia, Rivas, Elizabeth, Maidana, Alberto de Jesús Ortiz, Dolly Mabel Bordon Cabral, Marcelo, Simi, Cesar, Aponte, Juan Carlos Rivas, Sirley, Gill, Amilcar, Garcia, Gloria, Alvarenga, Laura, Cespedes, Hugo, Perez, Maria Liz Moreira, Fidelina, Canete, Roberto, Gonzalez, Natalia, Monges, Mary, Coman, Marcelo, Pederzani, Natalia, Franco, Ferdinand, Aganon, Regina, Martinez, Debbie, Noblezada-Uy, Chris Gerome Ellazar, Franklin Dean Cerezo, Jose Emmanuel Palo, Cristal April Jane Aperocho, Michael, Isanan, Marta, Tubacka, Przemyslaw, Jasiewicz, Miroslaw, Czuczwar, Michal, Borys, Aleksandra, Gutysz-Wojnicka, Lidia, Glinka, Ryszard, Gawda, Jan, Bilawicz, Paula, Cabrita, João, Vieira, Margarida Ferreira Figueiredo, Cristiana Mota Pinheiro, Nelson, Antunes, Laura, Pedro, Fatima, Ferreira, Isabel, Parente, Maria, Varela, Fatima, Fernandes, Claudia, Martins, Abel, Viveiros, Raquel, Cavaco, Clara Santa Rita, Sofia, Dias, Ana Margarida Feranandes, Pedro, Silva, Catarina, Nunes, João, Cabral, Filpe, Pires, Hilaryano, Ferreira, Jacinta, Santos, Vitor Manuel Vaz Pinto, Bruno Miguel Bispo, Amelia, Ferreira, Elena, Molinos, Estevão, Lafuente, Ricardo, Gregorio, Humberto, Costa, Ângela, Lima, Susana, Ferreira, Vanda, Seromenho, Eulália, Luis, Idália, Valerio, Helena, Cesar, Ana, Tavares, Ahmed Subhy Alsheikhly, Saeed, Mahmood, Catalin Traian Guran, Alida, Moise, Daniela Carmen Filipescu, Mihail, Luchian, Mihai, Popescu, Monica Adriana Scutariu, Cristina, Petrisor, Natalia, Hagau, Ioana, Grigoras, Tatiana, Patrichi, Vitaly, Gusarev, Alexandra, Pivkina, Vladimir, Kulakov, Olga, Ignatenko, Julia, Kovaleva, Trina, Zhivotneva, Marina, Zhedaeva, Nikita, Matiushkov, Olga, Ershova, Natalya, Egorova, Victoria, Khoronenko, Danil, Baskakov, Dmitry, Sergeev, Michael, Piradov, Liudmila, Grishina, Marat, Magomedov, Evgeniy, Zuev, Uri, Gorokhovatsky, Anna, Leonova, Liudmila, Fadeeva, Vladislav, Belskiy, Dmitriy, Galishevskiy, Nadezhda, Zubareva, Maksim, Tribulev, Oksana, Zueva, Alexander, Kiselev, Nikolaj, Kamenshchikov, Ekaterina, Tokareva, Maxim, Petrushin, Irina, Starchenko, Isaac, Nshimyumuremyi, Jerome, Muhizi, Egide, Buregeya, Josue, Nzarora, Amer, Assiri, Maie Salem Ebaid, Ghaleb, Almekhlafi, Yasser, Mandourah, Jelena, Velickovic, Dejan, Veličković, Bojan, Jovanovic, Adi, Hadzibegovic, Branislava, Stefanovic, Vanja, Misic, Vesna, Bumbasirevic, Marija, Rajković, Milena, Stojanovic, Srđan, Gavrilovic, Maja, Stanojević, Aktham, Yaghi, Anton, Turčan, Peter, Firment, Garri, Slobodianiuk, Daria, Rabarova, Danca, Lančaričová, Janko, Vlaovic, Matjaž, Groznik, Milica, Lukic, Janja, Perme, Maja, Sostaric, Nejc, Umek, Tomislav, Mirkovic, Simon, Dolenc, Misa, Fister, Nika, Zorko, Andrej, Markota, Nomhle Princess Yeni, Phumele, Jali, Shelley, Schmollgruber, Muhommed Ridwaan Syed, Nivisha, Parag, Robert, Wise, Maria, Galiana, José Alejandro Navarro, Ana María De Pablo, Patricia, Albert, Pilar, Martinez, Yolanda, Mendiara, Barbara, Garcia, Ana Alabart Llinas, Marilyn, Riveiro, Elisabet, Gallart, Alba, Riera, Miquel, Sanz, Swagotika, Salo, Miguel Angel Gimenez Lajara, Montserrat Venturas Nieto, Rosa, Garcia, José Manuel Garcia Pena, Maria Carmen Gorgolas, Maria Aranzazu Isasi, Rafael, Sierra, Federico, Gordo, Isabel, Conejo, Vicent, Salvà-Costa, Carolina, Garzón-Tovar, Sara, Lospitao, Rafael, Gonzalez, Pedro, Gutierrez, Mercè, Girona, Jordi, Adamuz, Pablo Garcia Olivares, José Peral Gutierrez de Ceballos, Celia, Tirado, Irene De Wit, Ana Belén Curto Polo, Maria Del Mar Diaz Salcedo, Javier, Ripolles-Melchor, Eugenio, Martinez-Hurtado, Jorge Duerto Alvarez, María Luisa Bravo Arcas, Juan Ignacio Torres Gonzalez, Ana Belén Sánchez de la Ventana, Pablo Lopez-Arcas Calleja, Raquel Garcia Alvarez, Purificacion Sanchez Zamora, Alvaro Ortega Guerrero, Rosario, Cosano, Jonathan, Perez-Vacas, Margarita, Campos-Perez, Emma Moreno Barreiro, Losune Cano Sanchez, Monica Garcia Diaz, Raquel, Jimenez, Lorena Del Rio Cabajo, Daniel Sancho Muriel, Helena Fernandez Alonso, Ana Wensell Fernández, Isabel Santín Piñan, Guillermo Muñiz Albaiceta, Maria Cristina Iglesias Fernandez, Francisco Javier Saenz Abos, Pablo, Monedero, Ramon Molina Chueca, Lydia Gallego Aguirre, Silvia Call Manosa, Carmen Partera Luque, Neus, Calpe, Monica Recio Losilla, Meritxell Tapia Fores, Olga, Farre, Oscar, Fernandez, M Del Rosario Villar Redondo, Donaldo, S Arteta Arteta, Maria Angeles Hurtado Sanchez, Cristina Paños Espinosa, Laura Martinez Reyes, Laura Claramunt Domenech, Carmen Velasco Guillén, Josep Trenado Alvarez, Mercedes Del Cotillo, Jesus Emilio Barrueco-Francioni, Belen Burgos Conde, Maria Pilar Sogues Blanco, Maria Luisa Blasco, Ana Isabel Clement, Clara, Hurtado, Luz Coronado Sanz, David, Perez-Torres, Estefanía, Prol-Silva, Jorge, Pereira, Iván Areán González, Anastasio Espejo Cano, Cesar Rodriguez Nuñez, Inmaculada Lorenzo Fernadez, Alejandra Azahara Marguello Fernandez, Rosa Del Bosque Diez, Badiola, Hilario, Begoña, Zalba-Etayo, Ana, Pascual-Bielsa, Preveen, Banwarie, Dick, Nahar, Alisha van Axel, Naraindath, N Boedjawan, Erika Backlund Jansson, Ann-Sofie, Malvemyr, Lotta, Johansson, Ulla, Sandberg, Catarina, Tingsvik, Gunilla, Mattsson, Gun, Löf, Martin, Spångfors, Mona, Ringdal, Sebastian, Geijer, Lotti, Orvelius, Mia, Hylen, Caroline, Lagerhäll, Eva, Åkerman, Viveca Hamback Hellkvist, Ulrica, Mickelsson, Ewa, Wahlbom, Ing-Marie, Larsson, Ewa, Wallin, Filippo, Boroli, Solenne, Ory, Margaret Lynn Jong, Alexander, Dullenkopf, Martin, Lang, Yvan, Fleury, Marianne, Maus, Nawfel, Ben-Hamouda, Anne, Fishman, Mei Yu Hsu, Shu Chuan Chang, Konlawij, Trongtratul, Chaiwut, Sawawiboon, Sunthiti, Morakul, Bodin, Khwannimit, Keevan, Singh, Dale, Ventour, Dianne, Figaro-Barclay, Sasha, Sankar-Maharaj, Mhamed Sami Mebazaa, Salma, Kamoun, Souheil, Elatrous, Lamia, Besbes, Fekri, Abroug, Walid, Naija, Youssef Zied Elhechmi, Walid, Sellami, Zied, Hajjej, Takoua, Merhabene, Imen, Talik, Ozlem Ozkan Kuscu, Ozcengiz, Dilek, Avşar, Zerman, Hayriye Cankar Dal, Sema, Turan, Semih, Aydemir, Hakan, Yilmaz, Duygu Kayar Calili, Seval, İzdes, Melike, Cengiz, Ayça, Gümüş, Banu, Taşdemir, Ali, Kağnıcı, Mustafa, Ay, Serap Avcı Ay, Gulbahar, Caliskan, Turkay, Akbas, Abidin Oner Balbay, Serdar, Efe, Volkan, Inal, Gülseren, Elay, Pınar, Karabacak, Boğaç, Özserezli, Evren, Şentürk, Oktay, Demirkiran, Suha, Bozbay, Elif, Erdogan, Mustafa, Akker, Nebia, Peker, Asu, Ozgultekin, Sibel Ocak Serin, Can, Turan, Gulsah, Karaoren, Senay, Goksu, Sait, Karakurt, Huseyin, Arikan, Fethi, Gül, İsmail, Cinel, Iskender, Kara, Hasan Nabi Undar, Yesim Serife Bayraktar, Jale Bengi Çelik, Murat Emre Tokur, Demet Tok Aydin, İsmail, Yildiz, Beysim, Özcan, Başar, Erdivanli, Ahmet, Eroglu, Devrim, Akdağ, Nurdan, Ünlü, Adonis, Dungca, Ashwaq, Ali, Bindu, Thankamma, Paul Eric Reyes, Sini, John, Ajitha, Rajendran, Fatima Kasem El Ahmad, Kathleen Ann Smiley, Susanna, Hojden, Mia Thorning Miller, Vishnu Das Sasidharan Nair, Maria Gracia San Antonio, Khaled Al Qawasmeh, Sabah Abu Shawish, Hilary, Twiggs, Ines, Rosado, Volodymyr, Babych, Faye, Morren, Charlotte, Young, Nicola, Vaughan-Jones, Stephanie, Harris, Karen, Burns, Carmel, Georgiev, Rosina, Shayamano, Ian, Kerslake, Peter, Creber, Ana, Vochin, Catherine, O'Brien, Paul, Caddell, Samantha, Hagan, Mandy, Hughes, Tomasz, Torlinski, James, Sherwin, Santhana, Kannan, Amber, Markham, Richard, Lebon, Jason, Cupitt, Julius, Cranshaw, Nigel, White, Victoria, Marriott, Wendy, Milner, Casiano Barrera Groba, Joao, Azoia, Petra, Polgarova, Shaly, George, Ritoo, Kapoor, Ceri, Lynch, Nathalie, Fox, Karen, Cranmer, Natalie, Fox, Thomas, Llewellym, Kelly, Matthews, Louise, Maltby, Jowena, Ibao, Karen, Boulton, Rachel, Jarman, Karen, Baxter, Ashok Sundai Raj, Arif, Moghal, Joanne, White, Suzanne, Barrowcliffe, Mark, Pulletz, Vaarisan, Ganeshalingam, Rosaleen, Baruah, Carole, Boulanger, Helen, Baker, Justin, Woods, Poe Poe Ei, Vongayi, Ogbeide, Paul, Hayden, Jennifer, Hughes, Madhu, Balasubramanian, Armorel, Salberg, Rajnish, Saha, Dagmar, Holmquist, Claire, Derbyshire, Neil, Smith, Elizabeth, Stones, Jane, Ademokun, Monica, Popescu, Maria Schofield Legorburo, Samantha, North, Carole, Brett, Helen, Jaundoo, Jayne, Craig, Simon, Whiteley, Clare, Howcroft, Liz, Wilby, Peter, Delve, David, Shaw, Karen, Williams, Ingeborg, D Welters, Jane, Mcmullen, Stephen, Brett, Leah, Flores, Treiza, Trueman-Dawkins, Mae, Templeton, John, Adams, Catherine, Smith, John, Prowle, Heather, Byers, Andrea, Mcdonnell, Bernd Oliver Rose, Rosie, Reece-Anthony, Luis, Mendes, Marcela, Vizcaychipi, Rhian, Bull, Grace, Lacaden, Eleanor, Santiago, Carlos Castro Delgado, Sarah, Farnell-Ward, Elaine, Thorpe, Justine, Somerville, Anne, Williams, Donna, Cummings, Helen, Derrick, Sarah, Brumwell, Claire, Randell, Nicola, Mccann, Emma, Aves, Gillian, Berry, Tamas, Szakmany, Una, Gunter, Paul, Pulak, Nikki, Sarkar, Kerry, Wright, Vitor, Gomes, Jones, Jo, Ruth, Palfrey, Julie, Camsooksai, Abby, Lewis, Antony, Eneas, Ascanio, Tridente, Louise, Barr, Beverley, Thomas, Emma, Parkin, Daniel, Horner, Christian, Frey, Suzanne, Bench, Rachel, Baumber, Phil, Broadhurst, Matthew, Jackson, Lynne, Williams, Michele, Clark, Jonathan, Paddle, Sarah, Bean, Sarah, Buckley, Christopher, Palfreeman, Sophie, Liu, Nicola, Allison, Ben, Attwood, Penny, Parsons, Victoria, Houghton, Sarah Jane Turner, David, Higgins, Egidija, Bielskute, Nicola, Horrigan, Reni, Jacob, Karen, Habgood, Ahmed, Zaki, Amy, Collins, Jenny, Lord, Charalice, Ramiro, Agnieszka, Kubisz-Pudelko, Michelle, Kotze, Helen, Williams, Ihor, Iovenko, Alexander, Tsarev, Arturo, Briva, Gabriela, Mendez, Lena, Napolitano, Magnus, Teig, Jarone, Lee, Gabriel Enrique Rodriguez, Talia, Ben-Jacob, Christopher, Potestio, Timothy, Eng, Dea, Mahanes, Ashish, Khanna, Abhijit, Duggal, Masakatsu, Nananmori, Manuel, Lois, Anna, Diaz, Kunal, Karamchandani, Cheryl, Bealer, Cindy, Barefield, Dorothy, Terry, Pauline, Fivecoat, Olakunle, Idowu, Juan, Cata, Tara, Clesi, Jessica, Peterson, Kevin, Hatton, Jasdeep, Dhaliwal, Dorothee, Mueller, Jing, Tao, Ashley Szabo Eltorai, Stephen, M Pastores, Natalie, Remor, Janel, Salazar, Diane, Barkas, Aaron, Joffe, Christopher, Barnes, Carrie, Sona, Marilyn, Schallom, Jack, Short, Javier, Lorenzo, Ingrid Von Der Osten, Marta, Borkowska, Mireia, Llaurado-Serra, Liesbet, Demarré, Vincianne, Pleitinckx, Cynthia, Xing, Anne-Sophie, Debue, Stefan, Goller, Elsa, Afonso, Gusarov, V, Eugeniya, Larina, Llaurado-Serra, M, Yalim, Dikmen, DecubICUs Study Team, European Society of Intensive Care Medicine (ESICM) Trials Group Collaborators, Muzha, D., Ribas, A.M., Lipovesty, F., Loudet, C., Coyer, F., Eller, P., Mostafa, N., Honoré, P.M., Telleria, V.M., Smajic, J., Nogueira, P.C., Nafees, KMK, Hentchoya, R., Rose, L., Soledad, J., Lin, F., Cardenas, Y., Reyes, A.G., Sustic, A., Mpouzika, M., Vymazal, T., Jensen, H.I., Aguirre-Bermeo, H., Maddison, L., Valta, M., Calvino-Gunther, S., Bloos, F., Adipa, F.E., Koulouras, V., Enamorado, J., Ágoston, Z., Birgisdóttir, H., Gupta, A., Gurjar, M., Kilapong, B., Hashemian, S.M., Martin-Loeches, I., Benbenishty, J., Cortegiani, A., Fletcher, K., Hayashi, Y., Waweru-Siika, W., Abidi, K., Lee, S.M., Hadri, B., Dolgusevs, M., Abillama, F.F., Jovaisa, T., Thix, C., Elhadi, M., Nor, B.M., Ratnam, S., Mazlan, M.Z., Maiyalagan, S., Sánchez-Hurtado, L., Belii, A., Naranpurev, M., Gautam, P., De Lange, D., Parke, R., Ilesanmi, R.E., Shosholcheva, M., Petosic, A., Lind, R., Ffarcsi, M.H., Bogarin, J., Hernandez, A.M., Mikaszewska-Sokolewicz, M., Sousa, B., Tomescu, D., Sandesc, D., Twagirumugabe, T., Gusarov, V., Ebaid, M., Jankovic, R., Slobodianiuk, G., Martonova, A., Knafelj, R., Mer, M., Maseda, E., Panka, B., Schefold, J.C., Joelsson-Alm, E., Trongtrakul, K., Merritt-Charles, L., Besbes, L.O., Dikmen, Y., Zgrzheblovska, L., Fielding, M., Rubulotta, F., Khanna, A.K., Saager, L., von der Osten, I., Greca, A., Cani, A., Xhindi, N., Hyska, G., Pinto, S., Alves, P., Esposito, R., Valgolio, E., Minope, JTS, Abdala, A., Ayala, M., Bravo, S., Bantar, A., Delgado, P., Badariotti, G., Lipovestky, F., Diaz, A., Saul, P., Setten, M., Aucapina, A., Acosta, Y., Gonzalez, V., Camputaro, L., Baccaro, F., Villa, R., Mastantuono, M., Dean, E., Rostello, O.F., Brizuela, P., Bartoli, J.R., Guereschi, M., Quiroga, C., Putruele, S., Villegas, P., Curilen, V., Fernandez, R., Nocheretti, M.G., Escalante, R.G., Loudet, C.I., Fernandez, S., Gonzalez, A.L., Alvarez, G.A., Iglesias, F., Chaparro, S., Zakalik, G., Pagella, G., Baini, M., Campos, P.A., Sabbag, I., Schmukler, A., Fonseca, I.P., Alvarez, G.M., Ramirez, M., Tapia, F., Bascary, C.A., Del Valle Gimenez, G., Bertoletti, F.P., Milioto, E., Bonsignore, PJM, Fernandez, M.A., Smith, J., Chimunda, T., Thompson, L., Maguire, T., Chaboyer, W., Watts, S., Mitchell, M., Powell, M., Lye, I., Parsons, L., Baker, N., Reynolds, C., Thompson, A., Masters, K., Sosnowski, K., Morrison, L., Leslie, G.D., Lakshmanan, R., Tabah, A., Brown, W., McDowell-Skaines, S., McLucas, A., Smith, C., Tallot, M., Jones, S., Barakat-Johnson, M., Leong, T., Butcher, R., Martin, K., Douschan, P., von Lewinski, D., Schmutz, R., Kolussi, U., Salman, F., Ateya, Z., De Decker, K., Van Regenmortel, N., Jans, A., Wijnands, P., Coremans, S., Honore, P.M., De Bels, D., Depuydt, T., Paillet, C., Jacquet, L.M., Swinnen, W., Hannes, F., Mergeay, M., Van de Velde, S., Allaert, S., Hoste, P., Borin, C., Balon, S., Fraipont, V., Biston, P., De Schryver, N., Dugernier, T., Van Cotthem, I., de Almeida, A.O., Jorge, S.A., Becker, D., Schmidt, R.C., Oliveira, E., Ramalho, A., Mazocoli, E., Fioretti, A., Barros, E., Serpa, L., Bianchini, S., Campanili, T., Pantaleao, T., Garcia, P.C., Ronchini, ALV, Santos, R., Manap, NBA, Bagshaw, S., Carney, D., Davidow, J., Rokosh, E., Laizner, A.M., Smith, S., McQuirter, M., Kampayana, B.S., Favre, R., Sills, M., Dallaire, J., Becker, C., Microys, S., Bowes, B., Lajeunesse, J., Ghosh, R., Baptiste-Savoie, J., Raizman, R., Suen, G., Taghavi, N., Smith, O., Fielding, C., Canales, J., Molina, P., Chaparro, J., Sepulveda, M.I., Zamorano, MJF, Rocha, P., Villanueva, X., Araya, P., Dayan, M., Avalos, F., Li, X., Liu, Y., Chen, X., Jiang, Z., Yang, J., Chen, J., Yang, L., Wang, K., Gao, J., Fang, X., Zhao, R., Xia, X., Liu, H., Li, J., Wang, H., Meng, G., Di, Y., Wang, D., Zhao, R.H., Hu, L.P., Peipei, X., Jiao, Q.F., Wang, H.Y., Xia, C.J., Ye, M., Wan, Y., Wang, W., Ding, Y., Ren, A., Gao, Y., Li, Q., Du, G., Shen, Y., Li, N., Yuan, C., Tan, L., Lin, Q., Guo, H., Yan, H., Xu, X., Zhang, W., Liang, J., Zhang, L., Tian, E., Zhao, Q., InSu, L., Dong, J., Gu, Y., Zhao, L., Qiao, H., Tuo, L., Lv, M., Zhu, J.Y., Zhu, J., Wei, Y., Liu, M., He, Y., Cheng, J., Liu, J., Jia, N., Wei, D., Wu, X., Duan, H., Lin, D., Liang, Q., Luo, X., Xiong, Y., Huang, R.F., Fu, J., Zan, T., Shi, Z., Long, Y., Lei, Y., Liu, X., Yumei, C., Wang, L., Zhang, Y., Xu, Y., Cheng, X., Zhijuan, W., Sun, C., Song, J., Wang, Y., Yuan, Y., Huang, Q., Yang, F., Wu, Y., Bai, X., Zheng, H., Song, M., Sun, Y., Li, Z., Luo, F., Li, L.C., Zhang, G., Xiao, L., Yu, T., Gao, G., Wei, W., Wang, F., Han, T., Li, T., Zeng, Q., Zeng, J.M., Pan, F., Wang, J., He, G., Chen, H., Zhang, F., Chao, Y., Chunhua, G., Yao, X., Bai, D., Liu, L., Liang, X., Zhang, N., Zhang, A., Hu, X., Zhang, H., Wang, R., Tak, P.S., Ho, S.W., Jiang, Q.X., Ding, X., Hong, L., Miao, L., Feng, Z., Huang, L., Wu, J., Guo, J., Zhang, B., Ma, C., Han, Y., Liu, C., Ding, M., Luan, L., Zheng, J., Lv, S., Jiang, S., Cao, W., Xue, X., Liu, G., Wei, X., Jiang, Y., Yao, Z., Gao, L., Zhao, W., Jiang, M., Hao, J., Zhang, J., Song, C., Chen, F., Wang, S., Hu, L., Cao, D., Wan, J., Wang, X., Shao, H., Zhang, Z., Cui, X., Fan, L., Yu, M., Li, B., Li, C., Chen, W., Li, Y., Zhigang, Z., Yuchen, W., Mu, C., Zhu, G., Bo, Q., Li, L., Chen, M., Jiang, J.H., Yin, H., Pang, X., Gong, Y.Y., Yang, S., Yan, X., Zheng, X., Lei, D., Lei, L., Guo, Y., Yu, J., Sun, W., Bi, A., Li, W., Ni, D., Wu, Z., Song, B., Fei, Q., Xiaoyan, Y., Ran, Q., Xixi, L., Jiao, X., Ji, H., Zhiping, S., Hong, M., Jianhong, M., Hao, Y., Yin, L., Hui, C., Ju, W., Huo, Y., Chen, L., Yan, Y., Bao, G., Cao, Y., Xu, L., Guixiang, J., Zhao, H.M., Huang, X., Dai, Z., Jian, Y., Tian, Z., Cao, Z.Q., Li, M., Ouyang, F., Ma, F., Jin, W., Ge, L., Wu, S., Yuan, W., Chen, T., Shi, G., Chen, Z., Liu, K., Lin, X., Yuemen, L., Lijuan, S., Tian, X.F., Dong, Y., Bocui, N., Wang, G., Zhao, Y., Yang, Q., Hu, R.L., Li, X.Q., Yu, Z.J., Yao, Y., Deng, X., Xiao, Y., Xie, Y., Yang, Y., Yang, H., Zhou, Y., Xiao, M., Tian, Y., Gama, LMS, Hernandez, J.S., Caicedo, N., Marin, J., Ochoa, M.E., Gomez, M., Rojas-Suarez, J., Gonzalez, J., Reyes, AJG, Chapeta, E., Orozco, E., Filipović-Grčić, I., Vuković, A., Pečenković, S., Šuput, A., Zivanovic-Posilovic, G., Bozena, A., Udiljak, N., Milic, M., Radivojevic, R.C., Mihaljevic, S., Matas, M., Tonkovic, D., Čuljak, H., Herceg, I., Pavlisa, G., Dobric, M., Beker, T., Adam, V.N., Goranovic, T., Markoulias, C., Mathaios, M., Mylordou, M., Achilleos, E., Kleanthous, P., Kotanidi, V., Foka, M., Charalabous, I., Alexandrou, A., Georgiou, M., Patsalos, A., Zepoy, S., Constantinou, C., Piza, P., Wiborg, E., Bruhn, L., Kaasby, K., Pedersen, K.R., Mikkelsen, S., Collet, M., Langvad, A., Andresen, H., Fischer, S., Kjærgård, I.E., Jepsen, B., Husted, B., Bestle, M., Kodal, A.M., Hansen, TCB, Pedersen, ASB, Thomsen, T.D., Hoegenhaven, A., From, M., Frandsen, T.M., Henning, G., Hansen, A., Bliksted, I.A., Tamayo, L.M., Mogrovejo, P., Palaez, C., Tutillo, DRM, Hurtado, C.V., García, M.F., Alvarez, D., Guerrero, F., Vasquez, A., Kütimets, M., Tamme, K., Anvelt, E., Dlamini-Sserumaga, L., Löfqvist, C., Lusenius, V., Kauppi, O., Sakki, J.K., Tervo-Heikkinen, T., Kesti, U., Merilainen, M., Karjula, E., Peltomaa, M., Palmu, A., Ahtiala, M., Valta, M.A., Mentec, H., Plantefève, G., Besch, G., Pili-Floury, S., Ledochowski, S., Déserts, MDD, Giacardi, C., Daubin, C., Massard, A., Le Guen, Y., Blanc, A., Mandaroux, S., Günther, S.C., Avogadro, P., Radavidson, A., Turc, J., Jochmans, S., Quintard, H., Boyer, L., Bruel, C., Philippart, F., Montravers, P., Atchade, E., Flessel, N., Chinardet, B., Soulisse, L., Pillard, C., Ngo, D., Bongiorno, B., Heitzler, N., Souppart, V., Gautheret, N., Timsit, J.F., Essardy, F., Fartoukh, M., Mehay, D., Etourneau, F., Farkas, J.C., Beuret, P., Preda, G., De Montmollin, E., Castelain, V., Jaschinski, U., Rothenfusser, M., Kindgen-Milles, D., Dimski, T., Fiedler, C., Heinicke, T., Meybohm, P., Schulze, T., Bota, M., Pelz, S., Odenthal, T., Christ, M., Bösl, K., Chovas, A., Stehr, S., Simon, P., Grotheer, S., Schüppel, S., Schaller, S., Albrecht, L., Stübner, A., Graeser, S., Kolbe, N., Lausch, M., Diers, A., Guenther, U., Riessen, R., Roller, M., Osei, I.P., Kusi-Appiah, A.C., Yakubu, Y.H., Guadi-Gosh, B., Dragoumanis, C., Christofis, C., Kazakos, N., Bastani, S., Martinos, C., Bekos, V., Papanikolaou, M., Papavasilopoulou, T., Efthymiou, A., Chantziara, V., Kyriakoudi, A., Kakaras, N., Diakaki, C., Flevari, A., Nikolaou, C., Katerina, K., Avramopoulou, L., Tsikritsaki, K., Gkiokas, G., Pantiora, E., Katsenos, C., Patsiou, E.C., Alexandropoulou, P., Koutsodimitropoulos, I., Farmakis, E., Nestora, K., Chatzis, M., Kondili, E., Soundoulounaki, S., Mousafiri, O., Lepida, D., Liarmakopoulou, A., Papathanakos, G., Oikonomou, M., Ioannides, P., Papadopoulos, D., Staikos, I., Stafylaraki, M., Raitsiou, B., Mandis, K., Ravani, I., Kourelea, S., Efthimiou, A., Thoma, G., Bakas, A., Psarulis, K., Anisoglou, S., Papageorgiou, E., Michailidou, E., Tholioti, T., Lavrentieva, A., Sourla, E., Spyropoulou, A., Pantelas, N., Stalika, KMM, Georgakas, I., Karathanou, A., Tsikriki, S., Dimoula, A., Kanakaki, S., Vakalos, A., Pagioulas, K., Enamorado, J.E., Nardai, G., Hawchar, F., Blondal, A., Rygvadottir, B., Jonasdottir, R.J., Birgisdottir, H., Shah, B., Kaushik, S., Tripathy, S., Singh, M., Agarwal, S., Gupta, M., Ahmad, M., Mangal, K., Bhargava, V., Kushare, V., Jha, S., Bhakhtiani, L., Kamal, M., Baronia, A., Susanti, A., Lestari, M.I., Zulkifli, Z., Baskoro, W., Zand, F., Zarei, F., Mahmoodpoor, A., Heidari, F., Jafaraghaee, F., O'Shea, A., O'Shea, F., O'Donnell, C., Craig, G., Fitzpatrick, G., Dunne, L., Hastings, J., Marsh, B., Cody, C., Campbell, E., Doyle, D., Pacturanan, M., Sheehan, C., Carey, A., Carter, C., Mulvey, R., Finn, DOR, Motherway, C., Walsh, A., Kehoe, J., Delossantos, S., Lalor, J., O'Nuallain, S., Behan, H., McPherson, S., Corcoran, A., Gordon, P., Rooney, G., Levy, D., Azencot, M., Gurevich, V., Lavy, A., Bendelari, V., Marconi, R., Barone, A., Gatti, C., Giampaoletti, A., Borgognoni, C., Ghioldi, D.M., Raimondo, A., Castiglione, G., Bruno, A.V., Rubulotta, G., Mo, A., Corso, A., Girianni, S., Bruni, A., Garofalo, E., Maggiore, S.M., Di Risio, A., Calamai, I., Spina, R., Spadaro, S., Volta, C.A., Cotoia, A., Mirabella, L., Maulicino, L., Abregal, G., Donvito, M., D'Ambrosio, P., Binda, F., Adamina, I., Galazzi, A., Negro, A., Vaschetto, R., Capuzzi, F., Boschetto, M., Stivanello, L., Bonaccorso, L., Megna, C., Iozzo, P., Rizzo, A., Scire, G., Taibi, M.R., Tranello, F.P., Manzo, A., Traina, L., Pastore, B., Quaini, A., Giusti, G.D., Montaldi, G., Piergentili, F., Mancini, F., Casaioli, S., Uccelli, F., Guarracino, F., Onelli, A., Di Gravio, V., Cossu, M., Matrona, O., Rocco, M., Alampi, D., Dellafiore, F., Ranalli, F., Bossolasco, M., Brizio, E., Migliorino, P., Cortellazi, P., Rosati, M., D'Ambrosio, F., Quagliotto, C., Roman-Pognuz, E., Peratoner, A., De Rosa, S., Martin, M.A., De Sanctis, F., Ciorba, P., Toppin, P., Harding-Goldson, H., Taito, S., Shime, N., Yamamoto, R., Kanda, F., Hirao, A., Egi, M., Noguchi, A., Hashimoto, S., Aya, U., Sakuramoto, H., Ohuchi, A., Kataoka, J., Maruyama, K., Nakayama, I., Nishime, Y., Fujimoto, K., Takahashi, K., Tsujimoto, M., Shimizu, M., Tole, E., Correia, M.C., Kim, J.H., Park, S., Kim, K.C., Baek, J., Bae, J.M., Park, S.Y., Park, T.S., Lee, H.B., Park, J., Yeon-Joo, L., Young-Jae, C., Jeon, K., Kim, S.C., Lee, J., Chee, H.K., Huh, J.W., Sim, Y.S., Kim, J., Chang, Y., Ahn, J.J., Kang, B.J., Lee, W.Y., Lee, S.J., Baftiu, N., Krastins, I., Stiban, S., Feghaly, M.E., Gharios, E., Merheb, M., Benlamin, M., Khaled, A., Belkhair, W.A., Tabib, M., Ashour, F., Elhadi, A., Tababa, OWE, Khaled, T., Alkhumsi, SIR, Alshrif, A.I., Aboufray, A.A., Alabuzidi, A., Triki, A.R., Elgammudi, M., Zahra, H.B., Soula, E., Al-Alawi, MMS, Ahmed, H., Ghula, MAA, Vosylius, S., Mouton, L., Rastegar, T., Sertznig, C., Martin, G., Theisen, C., Ferretti, C., Gils, F., Gallion, M., Zainudin, A., Bahrin, LKK, Deva, S.R., Rahim, AHA, Wahab, S., Hassan, WNW, Ismail, WNW, Ali, M.N., Khoo, T.M., Samat, N.M., Tong, JMG, Adib, NAN, Nor, MBM, Ismail, N., Sulaiman, S.R., Foong, K.W., Alias, A., Hua, N.P., Zermeno, J.M., Blanco, D., Duran, K., Nava, CLL, Nandyelly, SJR, Sanchez-Hurtado, L.A., Tejeda-Huezo, B., Del Moral Armengol, M., Nava, LPA, Herrera, J.G., de Anda, GFV, Gallegos-Perez, H., Hernandez-Sanchez, N., Hernandez-Ponce, L., Gorordo-Delsol, L., Hernandez-Romero, M., Gomez, S., Molinar, F., Ñamendys-Silva, S.A., Romero-Gonzalez, J.P., Gonzalez, D., Landaverde, A., Sosa, M.Á., Navarro, B., de Molina Serrano, JIR, Iburrigarro, S.R., Ibarra, A., Aguirre, J., Martinez-Gonzalez, M., Padilla, NRC, Pineda, AAV, Villafuerte, MVE, Herrera, MOG, Baasanjav, B., Hachimi, A., Elkhayari, M., Dendane, T., Subedi, N.B., Pathak, S.D., Manandhar, M., Van Gulik, L., Van Den Brink, M., Van Vliet, P., Gerretsen, B., Van Den Berg, L., De Haan, M., Tuinstra, B., Kuijpers, P., Reijntjens, J., Vermeijden, J.W., Rinket, M., Vanroest, M., Reidinga, A., Loef, B., Dieperink, W., Onrust, M., Dormans, T., Bormans, L., Koopmans, M., Gerritsen, R.T., Van Den Elst, A., Evers, M., Oiting, O., Wilting, R., Ramaker, B., van der Kuil, M., Fijen, J.W., Haas, L., Haringman, J., Newby, L., Gilder, E., Hacking, D., Dagooc, R., Song, R., Waibel, H., Dawn, F., Rapley, J., Chadwick, L., Chapman, C., Crone, P., Albrett, J., Marko, P., Goodson, J., Browne, T., Whitticase, R., Davidson, C., Judd, H., Owens, D., Onyeka, T., Ugwu, I., Ilesanmi, R., Adejumo, P.O., Owojuyigbe, A., Adenekan, A., Uba, S., Chime, C., Jibrin, D., Sankey, B.J., Adekola, O., Olanipekun, S., Shosolcheva, M., Gievski, V., Kartalov, A., Naumovski, F., Kuzmanovska, B., Trposak, A., Bogoevska-Miteva, Z., Rosalia, R., Olsen, B.F., Sjobo, B., Jensen, K.D., Sykehus, D., Johansen, B.F., Straede, E., Johansen, E., Finnstrom, I.J., Toellefsen, A., Ostenjo, H., Bjorgen, H., Bratsberg, B., Kristoffersen, E., Skorstad, E.M., Hansen, S., Vullum, S., Lunde, G.A., Arntsen, W., Lund, M., Akselsen, G.R., Monstad, K.R., Stenset, A., Haugom, H., Monsen, B., Høgvall, L., Trudvang, S., Galaaen, B., Malmin, S.K., Andersen, M.H., Hargott, R.F., Andersen, Y., Steffenak, E., Nyhus, M., Meland, B., Hashmi, M., Rivas, N., Maidana, E., de Jesús Ortiz, A., Cabral, DMB, Simi, M., Aponte, C., Rivas, J.C., Gill, S., Garcia, A., Alvarenga, G., Cespedes, L., Perez, H., Moreira, M.L., Canete, F., Gonzalez, R., Monges, N., Coman, M., Pederzani, M., Franco, N., Aganon, F., Martinez, R., Noblezada-Uy, D., Ellazar, C.G., Cerezo, F.D., Palo, J.E., Aperocho, CAJ, Isanan, M., Tubacka, M., Jasiewicz, P., Czuczwar, M., Borys, M., Gutysz-Wojnicka, A., Glinka, L., Gawda, R., Bilawicz, J., Cabrita, P., Vieira, J., Figueiredo, M.F., Pinheiro, C.M., Antunes, N., Pedro, L., Ferreira, F., Parente, I., Varela, M., Fernandes, F., Martins, C., Viveiros, A., Cavaco, R., Rita, C.S., Dias, S., Feranandes, A.M., Silva, P., Nunes, C., Cabral, J., Pires, F., Ferreira, H., Santos, J., Pinto, VMV, Bispo, B.M., Ferreira, A., Molinos, E., Lafuente, E., Gregorio, R., Costa, H., Lima, Â., Ferreira, S., Seromenho, V., Luis, E., Valerio, I., Cesar, H., Tavares, A., Alsheikhly, A.S., Mahmood, S., Guran, C.T., Moise, A., Filipescu, D.C., Luchian, M., Popescu, M., Scutariu, M.A., Petrisor, C., Hagau, N., Grigoras, I., Patrichi, T., Gusarev, V., Pivkina, A., Kulakov, V., Ignatenko, O., Kovaleva, J., Zhivotneva, T., Zhedaeva, M., Matiushkov, N., Ershova, O., Egorova, N., Khoronenko, V., Baskakov, D., Sergeev, D., Piradov, M., Grishina, L., Magomedov, M., Zuev, E., Gorokhovatsky, U., Leonova, A., Fadeeva, L., Belskiy, V., Galishevskiy, D., Zubareva, N., Tribulev, M., Zueva, O., Kiselev, A., Kamenshchikov, N., Tokareva, E., Petrushin, M., Starchenko, I., Nshimyumuremyi, I., Muhizi, J., Buregeya, E., Nzarora, J., Assiri, A., Ebaid, M.S., Almekhlafi, G., Mandourah, Y., Velickovic, J., Veličković, D., Jovanovic, B., Hadzibegovic, A., Stefanovic, B., Misic, V., Bumbasirevic, V., Rajković, M., Stojanovic, M., Gavrilovic, S., Stanojević, M., Yaghi, A., Turčan, A., Firment, P., Rabarova, D., Lančaričová, D., Vlaovic, J., Groznik, M., Lukic, M., Perme, J., Sostaric, M., Umek, N., Mirkovic, T., Dolenc, S., Fister, M., Zorko, N., Markota, A., Yeni, N.P., Jali, P., Schmollgruber, S., Syed, M.R., Parag, N., Wise, R., Galiana, M., Navarro, J.A., De Pablo, A.M., Albert, P., Martinez, P., Mendiara, Y., Garcia, B., Llinas, A.A., Riveiro, M., Gallart, E., Riera, A., Sanz, M., Salo, S., Lajara, MAG, Nieto, M.V., Garcia, R., Pena, JMG, Gorgolas, M.C., Isasi, M.A., Sierra, R., Gordo, F., Conejo, I., Salvà-Costa, V., Garzón-Tovar, C., Lospitao, S., Gutierrez, P., Girona, M., Adamuz, J., Olivares, P.G., de Ceballos, JPG, Tirado, C., De Wit, I., Polo, ABC, Del Mar Diaz Salcedo, M., Ripolles-Melchor, J., Martinez-Hurtado, E., Alvarez, J.D., Arcas, MLB, Gonzalez, JIT, de la Ventana, ABS, Calleja, P.L., Alvarez, R.G., Zamora, P.S., Guerrero, A.O., Cosano, R., Perez-Vacas, J., Campos-Perez, M., Barreiro, E.M., Sanchez, L.C., Diaz, M.G., Jimenez, R., Del Rio Cabajo, L., Muriel, D.S., Alonso, H.F., Fernández, A.W., Piñan, I.S., Albaiceta, G.M., Fernandez, MCI, Abos, FJS, Monedero, P., Chueca, R.M., Aguirre, L.G., Manosa, S.C., Luque, C.P., Calpe, N., Losilla, M.R., Fores, M.T., Farre, O., Fernandez, O., Del Rosario Villar Redondo, M., Arteta Arteta, D.S., Sanchez, MAH, Espinosa, C.P., Reyes, L.M., Domenech, L.C., Guillén, C.V., Alvarez, J.T., Del Cotillo, M., Barrueco-Francioni, J.E., Conde, B.B., Blanco, MPS, Blasco, M.L., Clement, A.I., Hurtado, C., Sanz, L.C., Perez-Torres, D., Prol-Silva, E., Pereira, J., González, I.A., Cano, A.E., Nuñez, C.R., Fernadez, I.L., Fernandez, AAM, Del Bosque Diez, R., Hilario, B., Zalba-Etayo, B., Pascual-Bielsa, A., Banwarie, P., Nahar, D., van Axel, A., Boedjawan, N.N., Jansson, E.B., Malvemyr, A.S., Johansson, L., Sandberg, U., Tingsvik, C., Mattsson, G., Löf, G., Spångfors, M., Ringdal, M., Geijer, S., Orvelius, L., Hylen, M., Lagerhäll, C., Åkerman, E., Hellkvist, V.H., Mickelsson, U., Wahlbom, E., Larsson, I.M., Wallin, E., Boroli, F., Ory, S., Jong, M.L., Dullenkopf, A., Lang, M., Fleury, Y., Maus, M., Ben-Hamouda, N., Fishman, A., Hsu, M.Y., Chang, S.C., Trongtratul, K., Sawawiboon, C., Morakul, S., Khwannimit, B., Singh, K., Ventour, D., Figaro-Barclay, D., Sankar-Maharaj, S., Mebazaa, M.S., Kamoun, S., Elatrous, S., Besbes, L., Abroug, F., Naija, W., Elhechmi, Y.Z., Sellami, W., Hajjej, Z., Merhabene, T., Talik, I., Kuscu, O.O., Dilek, O., Zerman, A., Dal, H.C., Turan, S., Aydemir, S., Yilmaz, H., Calili, D.K., İzdes, S., Cengiz, M., Gümüş, A., Taşdemir, B., Kağnıcı, A., Ay, M., Ay, S.A., Caliskan, G., Akbas, T., Balbay, A.O., Efe, S., Inal, V., Elay, G., Karabacak, P., Özserezli, B., Şentürk, E., Demirkiran, O., Bozbay, S., Erdogan, E., Akker, M., Peker, N., Ozgultekin, A., Serin, S.O., Turan, C., Karaoren, G., Goksu, S., Karakurt, S., Arikan, H., Gül, F., Cinel, İ., Kara, I., Undar, H.N., Bayraktar, Y.S., Çelik, J.B., Tokur, M.E., Aydin, D.T., Yildiz, İ., Özcan, B., Erdivanli, B., Eroglu, A., Akdağ, D., Ünlü, N., Dungca, A., Ali, A., Thankamma, B., Reyes, P.E., John, S., Rajendran, A., Ahmad, FKE, Smiley, K.A., Hojden, S., Miller, M.T., Das Sasidharan Nair, V., Antonio, MGS, Qawasmeh, K.A., Shawish, S.A., Twiggs, H., Rosado, I., Babych, V., Morren, F., Young, C., Vaughan-Jones, N., Harris, S., Burns, K., Georgiev, C., Shayamano, R., Kerslake, I., Creber, P., Vochin, A., O'Brien, C., Caddell, P., Hagan, S., Hughes, M., Torlinski, T., Sherwin, J., Kannan, S., Markham, A., Lebon, R., Cupitt, J., Cranshaw, J., White, N., Marriott, V., Milner, W., Groba, C.B., Azoia, J., Polgarova, P., George, S., Kapoor, R., Lynch, C., Fox, N., Cranmer, K., Llewellym, T., Matthews, K., Maltby, L., Ibao, J., Boulton, K., Jarman, R., Baxter, K., Raj, A.S., Moghal, A., White, J., Barrowcliffe, S., Pulletz, M., Ganeshalingam, V., Baruah, R., Boulanger, C., Baker, H., Woods, J., Ei, P.P., Ogbeide, V., Hayden, P., Hughes, J., Balasubramanian, M., Salberg, A., Saha, R., Holmquist, D., Derbyshire, C., Smith, N., Stones, E., Ademokun, J., Legorburo, M.S., North, S., Brett, C., Jaundoo, H., Craig, J., Whiteley, S., Howcroft, C., Wilby, L., Delve, P., Shaw, D., Williams, K., Welters, I.D., McMullen, J., Brett, S., Flores, L., Trueman-Dawkins, T., Templeton, M., Adams, J., Prowle, J., Byers, H., McDonnell, A., Rose, B.O., Reece-Anthony, R., Mendes, L., Vizcaychipi, M., Bull, R., Lacaden, G., Santiago, E., Delgado, C.C., Farnell-Ward, S., Thorpe, E., Somerville, J., Williams, A., Cummings, D., Derrick, H., Brumwell, S., Randell, C., McCann, N., Aves, E., Berry, G., Szakmany, T., Gunter, U., Pulak, P., Sarkar, N., Wright, K., Gomes, V., Jones, J., Palfrey, R., Camsooksai, J., Lewis, A., Eneas, A., Tridente, A., Barr, L., Thomas, B., Parkin, E., Horner, D., Frey, C., Bench, S., Baumber, R., Broadhurst, P., Jackson, M., Williams, L., Clark, M., Paddle, J., Bean, S., Buckley, S., Palfreeman, C., Liu, S., Allison, N., Attwood, B., Parsons, P., Houghton, V., Turner, S.J., Higgins, D., Bielskute, E., Horrigan, N., Jacob, R., Habgood, K., Zaki, A., Collins, A., Lord, J., Ramiro, C., Kubisz-Pudelko, A., Kotze, M., Williams, H., Iovenko, I., Tsarev, A., Briva, A., Mendez, G., Napolitano, L., Teig, M., Rodriguez, G.E., Ben-Jacob, T., Potestio, C., Eng, T., Mahanes, D., Khanna, A., Duggal, A., Nananmori, M., Lois, M., Karamchandani, K., Bealer, C., Barefield, C., Terry, D., Fivecoat, P., Idowu, O., Cata, J., Clesi, T., Peterson, J., Hatton, K., Dhaliwal, J., Mueller, D., Tao, J., Eltorai, A.S., Pastores, S.M., Remor, N., Salazar, J., Barkas, D., Joffe, A., Barnes, C., Sona, C., Schallom, M., Short, J., Lorenzo, J., Von Der Osten, I., Borkowska, M., Llaurado-Serra, M., Demarré, L., Pleitinckx, V., Xing, C., Debue, A.S., Goller, S., Afonso, E., Larina, E., Labeau, Sonia O. [0000-0003-3863-612X], Afonso, Elsa [0000-0003-0873-0852], Benbenishty, Julie [0000-0002-8488-9649], Blackwood, Bronagh [0000-0002-4583-5381], Boulanger, Carole [0000-0002-1392-6633], Brett, Stephen J. [0000-0003-4545-8413], Calvino-Gunther, Silvia [0000-0003-3586-7205], Chaboyer, Wendy [0000-0001-9528-7814], Coyer, Fiona [0000-0002-8467-0081], Deschepper, Mieke [0000-0001-6797-3147], Honore, Patrick M. [0000-0002-6697-4890], Jankovic, Radmilo [0000-0003-0742-8686], Khanna, Ashish K. [0000-0002-9083-891X], Llaurado-Serra, Mireia [0000-0002-5123-0581], Lin, Frances [0000-0001-8735-5469], Rose, Louise [0000-0003-1700-3972], Rubulotta, Francesca [0000-0001-8644-1728], Saager, Leif [0000-0003-3416-4727], Williams, Ged [0000-0002-7481-2445], Blot, Stijn I. [0000-0003-2145-0345], Apollo - University of Cambridge Repository, Critical Care, Labeau S.O., Afonso E., Benbenishty J., Blackwood B., Boulanger C., Brett S.J., Calvino-Gunther S., Chaboyer W., Coyer F., Deschepper M., Francois G., Honore P.M., Jankovic R., Khanna A.K., Llaurado-Serra M., Lin F., Rose L., Rubulotta F., Saager L., Williams G., Blot S.I., Muzha D., Ribas A.M., Lipovesty F., Loudet C., Eller P., Mostafa N., Telleria V.M., Smajic J., Nogueira P.C., Nafees K.M.K., Hentchoya R., Soledad J., Cardenas Y., Reyes A.G., Sustic A., Mpouzika M., Vymazal T., Jensen H.I., Aguirre-Bermeo H., Maddison L., Valta M., Bloos F., Adipa F.E., Koulouras V., Enamorado J., Agoston Z., Birgisdottir H., Gupta A., Gurjar M., Kilapong B., Hashemian S.M., Martin-Loeches I., Cortegiani A., Fletcher K., Hayashi Y., Waweru-Siika W., Abidi K., Lee S.-M., Hadri B., Dolgusevs M., Abillama F.F., Jovaisa T., Thix C., Elhadi M., Nor B.M., Ratnam S., Mazlan M.Z., Maiyalagan S., Sanchez-Hurtado L., Belii A., Naranpurev M., Gautam P., De lange D., Parke R., Ilesanmi R.E., Shosholcheva M., Petosic A., Lind R., Ffarcsi M.H., Bogarin J., Hernandez A.M., Mikaszewska-Sokolewicz M., Sousa B., Tomescu D., Sandesc D., Twagirumugabe T., Gusarov V., Ebaid M., Slobodianiuk G., Martonova A., Knafelj R., Mer M., Maseda E., Panka B., Schefold J.C., Joelsson-Alm E., Trongtrakul K., Merritt-Charles L., Besbes L.O., Dikmen Y., Zgrzheblovska L., Fielding M., von der Osten I., Greca A., Cani A., Xhindi N., Hyska G., Pinto S., Alves P., Esposito R., Valgolio E., Minope J.T.S., Abdala A., Ayala M., Bravo S., Bantar A., Delgado P., Badariotti G., Lipovestky F., Diaz A., Saul P., Setten M., Aucapina A., Acosta Y., Gonzalez V., Camputaro L., Baccaro F., Villa R., Mastantuono M., Dean E., Rostello O.F., Brizuela P., Bartoli J.R., Guereschi M., Quiroga C., Putruele S., Villegas P., Curilen V., Fernandez R., Nocheretti M.G., Escalante R.G., Loudet C.I., Fernandez S., Gonzalez A.L., Alvarez G.A., Iglesias F., Chaparro S., Zakalik G., Pagella G., Baini M., Campos P.A., Sabbag I., Schmukler A., Fonseca I.P., Alvarez G.M., Ramirez M., Tapia F., Bascary C.A., del Valle Gimenez G., Bertoletti F.P., Milioto E., Bonsignore P.J.M., Fernandez M.A., Smith J., Chimunda T., Thompson L., Maguire T., Watts S., Mitchell M., Powell M., Lye I., Parsons L., Baker N., Reynolds C., Thompson A., Masters K., Sosnowski K., Morrison L., Leslie G.D., Lakshmanan R., Tabah A., Brown W., McDowell-Skaines S., McLucas A., Smith C., Tallot M., Jones S., Barakat-Johnson M., Leong T., Butcher R., Martin K., Douschan P., von Lewinski D., Schmutz R., Kolussi U., Salman F., Ateya Z., De Decker K., Van Regenmortel N., Jans A., Wijnands P., Coremans S., De Bels D., Depuydt T., Paillet C., Jacquet L.-M., Swinnen W., Hannes F., Mergeay M., Van de Velde S., Allaert S., Hoste P., Borin C., Balon S., Fraipont V., Biston P., De Schryver N., Dugernier T., Van Cotthem I., de Almeida A.O., Jorge S.A., Becker D., Schmidt R.C., Oliveira E., Ramalho A., Mazocoli E., Fioretti A., Barros E., Serpa L., Bianchini S., Campanili T., Pantaleao T., Garcia P.C., Ronchini A.L.V., Santos R., Manap N.B.A., Bagshaw S., Carney D., Davidow J., Rokosh E., Laizner A.M., Smith S., McQuirter M., Kampayana B.S., Favre R., Sills M., Dallaire J., Becker C., Microys S., Bowes B., Lajeunesse J., Ghosh R., Baptiste-Savoie J., Raizman R., Suen G., Taghavi N., Smith O., Fielding C., Canales J., Molina P., Chaparro J., Sepulveda M.I., Zamorano M.J.F., Rocha P., Villanueva X., Araya P., Dayan M., Avalos F., Li X., Liu Y., Chen X., Jiang Z., Yang J., Chen J., Yang L., Wang K., Gao J., Fang X., Zhao R., Xia X., Liu H., Li J., Wang H., Meng G., Di Y., Wang D., Zhao R.H., Hu L.P., Peipei X., Jiao Q.F., Wang H.Y., Xia C.J., Ye M., Wan Y., Wang W., Ding Y., Ren A., Gao Y., Li Q., Du G., Shen Y., Li N., Yuan C., Tan L., Lin Q., Guo H., Yan H., Xu X., Zhang W., Liang J., Zhang L., Tian E., Zhao Q., InSu L., Dong J., Gu Y., Zhao L., Qiao H., Tuo L., Lv M., Zhu J.Y., Zhu J.F., Wei Y., Liu M., He Y., Cheng J., Liu J., Jia N., Wei D., Wu X., Duan H., Lin D., Liang Q., Luo X., Xiong Y., Huang R.F., Fu J., Zan T., Shi Z., Long Y., Lei Y., Liu X., Yumei C., Wang L.L., Zhang Y., Xu Y., Cheng, Zhijuan W., Sun C., Song J.H., Wang Y., Liu X.M., Yuan Y., Huang Q., Yang F., Wu Y., Bai X., Zheng H., Song M., Sun Y., Li Z.S., Luo F., Li L.C., Zhang G., Xiao L., Yu T., Gao G., Wei W., Wang F., Han T., Li T., Zeng Q., Zeng J.M., Pan F., Wang J., He G., Chen H., Zhang F., Chao Y., Chunhua G., Yao X., Bai D., Liu L., Liang X., Zhang N., Zhang A., Hu X.C., Zhang H., Wang R.X., Tak P.S., Ho S.W., Jiang Q.X., Ding X., Hong L., Miao L., Feng Z., Huang L.P., Wu J., Guo J., Zhang B., Ma C., Han Y., Liu C., Ding M., Luan L., Zheng J., Lv S., Jiang S., Cao W., Xue X., Liu G., Wei X., Jiang Y., Yao Z., Gao L., Zhao W., Jiang M., Hao J., Zhang J., Song C., Chen F., Wang S., Hu L., Cao D., Wan J., Wang X., Shao H., Zhang Z., Cui X., Fan L., Yu M., Li B., Li C., Liu X.L., Chen W., Li Y., Zhigang Z., Yuchen W., Mu C., Zhu G., Bo Q., Li L., Chen M., Jiang J.H., Yin H., Pang X., Gong Y.Y., Yang S., Yan X., Zheng X., Lei D.H., Lei L., Guo Y., Yu J., Sun W., Bi A.P., Li W., Ni D., Wu Z., Song B., Fei Q., Xiaoyan Y., Ran Q., Xixi L., Jiao X., Ji H., Zhiping S., Hong M., Jianhong M., Hao Y., Yin L., Hui C., Ju W., Huo Y., Chen L., Yan Y., Bao G., Cao Y., Xu L., Guixiang J., Zhao H.M., Huang X., Dai Z., Jian Y., Tian Z., Cao Z.Q., Li M., Ouyang F., Ma F., Jin W., Ge L., Wu S.F., Yuan W., Chen T., Shi G., Chen Z., Liu K., Lin X., Yuemen L., Lijuan S., Tian X.F., Feng Z.X., Liu X.Z., Dong Y., Bocui N., Jiang Z.X., Wang G.X., Zhao Y., Yang Q., Hu R.L., Li X.Q., Yu Z.J., Yao Y., Deng X., Xiao Y., Xie Y., Yang Y., Yang H., Zhou Y., Li Z., Xiao M., Yang Y.X., Tian Y., Gama L.M.S., Hernandez J.S., Caicedo N., Marin J., Ochoa M.-E., Gomez M., Rojas-Suarez J., Gonzalez J., Reyes A.J.G., Chapeta E., Orozco E., Filipovic-Grcic I., Vukovic A., Pecenkovic S., Suput A., Zivanovic-Posilovic G., Bozena A., Udiljak N., Milic M., Radivojevic R.C., Mihaljevic S., Matas M., Tonkovic D., Culjak H., Herceg I., Pavlisa G., Dobric M., Beker T., Adam V.N., Goranovic T., Markoulias C., Mathaios M., Mylordou M., Achilleos E., Kleanthous P., Kotanidi V., Foka M., Charalabous I., Alexandrou A., Georgiou M., Patsalos A., Zepoy S., Constantinou C., Piza P., Wiborg E., Bruhn L., Kaasby K., Pedersen K.R., Mikkelsen S., Collet M., Langvad A., Andresen H., Fischer S., Kjaergard I.E., Jepsen B., Husted B., Bestle M., Kodal A.M., Hansen T.C.B., Pedersen A.S.B., Thomsen T.D., Hoegenhaven A., From M., Frandsen T.M., Henning G., Hansen A., Bliksted I.A., Tamayo L.M., Mogrovejo P., Palaez C., Tutillo D.R.M., Hurtado C.V., Garcia M.F., Alvarez D., Guerrero F., Vasquez A., Kutimets M., Tamme K., Anvelt E., Dlamini-Sserumaga L., Lofqvist C., Lusenius V., Kauppi O., Sakki J.-K., Tervo-Heikkinen T., Kesti U., Merilainen M., Karjula E., Peltomaa M., Palmu A., Ahtiala M., Valta M.A., Mentec H., Plantefeve G., Besch G., Pili-Floury S., Ledochowski S., Deserts M.D., Giacardi C., Daubin C., Massard A., Le Guen Y., Blanc A., Mandaroux S., Gunther S.C., Avogadro P., Radavidson A., Turc J., Jochmans S., Quintard H., Boyer L., Bruel C., Philippart F., Montravers P., Atchade E., Flessel N., Chinardet B., Soulisse L., Pillard C., Ngo D., Bongiorno B., Heitzler N., Souppart V., Gautheret N., Timsit J.-F., Essardy F., Fartoukh M., Mehay D., Etourneau F., Farkas J.-C., Beuret P., Preda G., De Montmollin E., Castelain V., Jaschinski U., Rothenfusser M., Kindgen-Milles D., Dimski T., Fiedler C., Heinicke T., Meybohm P., Schulze T., Bota M., Pelz S., Odenthal T., Christ M., Bosl K., Chovas A., Stehr S., Simon P., Grotheer S., Schuppel S., Schaller S., Albrecht L., Stubner A., Graeser S., Kolbe N., Lausch M., Diers A., Guenther U., Riessen R., Roller M., Osei I.P., Kusi-Appiah A.-C., Yakubu Y.H., Guadi-Gosh B., Dragoumanis C., Christofis C., Kazakos N., Bastani S., Martinos C., Bekos V., Papanikolaou M., Papavasilopoulou T., Efthymiou A., Chantziara V., Kyriakoudi A., Kakaras N., Diakaki C., Flevari A., Nikolaou C., Katerina K., Avramopoulou L., Tsikritsaki K., Gkiokas G., Pantiora E., Katsenos C., Patsiou E.-C., Alexandropoulou P., Koutsodimitropoulos I., Farmakis E., Nestora K., Chatzis M., Kondili E., Soundoulounaki S., Mousafiri O., Lepida D., Liarmakopoulou A., Papathanakos G., Oikonomou M., Ioannides P., Papadopoulos D., Staikos I., Stafylaraki M., Raitsiou B., Mandis K., Ravani I., Kourelea S., Efthimiou A., Thoma G., Bakas A., Psarulis K., Anisoglou S., Papageorgiou E., Michailidou E., Tholioti T., Lavrentieva A., Sourla E., Spyropoulou A., Pantelas N., Stalika K.M.M., Georgakas I., Karathanou A., Tsikriki S., Dimoula A., Kanakaki S., Vakalos A., Pagioulas K., Enamorado J.E., Nardai G., Hawchar F., Blondal A., Rygvadottir B., Jonasdottir R.J., Shah B., Kaushik S., Tripathy S., Singh M., Agarwal S., Gupta M., Ahmad M., Mangal K., Bhargava V., Kushare V., Jha S., Bhakhtiani L., Kamal M., Baronia A., Susanti A., Lestari M.I., Zulkifli Z., Baskoro W., Zand F., Zarei F., Mahmoodpoor A., Heidari F., Jafaraghaee F., O'Shea A., O'Shea F., O'Donnell C., Craig G., Fitzpatrick G., Dunne L., Hastings J., Marsh B., Cody C., Campbell E., Doyle D., Pacturanan M., Sheehan C., Carey A., Carter C., Mulvey R., Finn D.O.C.R., Motherway C., Walsh A., Kehoe J., Delossantos S., Lalor J., O'Nuallain S., Behan H., McPherson S., Corcoran A., Gordon P., Rooney G., Levy D., Azencot M., Gurevich V., Lavy A., Bendelari V., Marconi R., Barone A., Gatti C., Giampaoletti A., Borgognoni C., Ghioldi D.M., Raimondo A., Castiglione G., Bruno A.V., Rubulotta G., Mo A., Corso A., Girianni S., Bruni A., Garofalo E., Maggiore S.M., Di Risio A., Calamai I., Spina R., Spadaro S., Volta C.A., Cotoia A., Mirabella L., Maulicino L., Abregal G., Donvito M., D'Ambrosio P., Binda F., Adamina I., Galazzi A., Negro A., Vaschetto R., Capuzzi F., Boschetto M., Stivanello L., Bonaccorso L., Megna C., Iozzo P., Rizzo A., Scire G., Taibi M.R., Tranello F.P., Manzo A., Traina L., Pastore B., Quaini A., Giusti G.D., Montaldi G., Piergentili F., Mancini F., Casaioli S., Uccelli F., Guarracino F., Onelli A., Di Gravio V., Cossu M., Matrona O., Rocco M., Alampi D., Dellafiore F., Ranalli F., Bossolasco M., Brizio E., Migliorino P., Cortellazi P., Rosati M., D'Ambrosio F., Quagliotto C., Roman-Pognuz E., Peratoner A., De Rosa S., Martin M.A., De Sanctis F., Ciorba P., Toppin P., Harding-Goldson H., Taito S., Shime N., Yamamoto R., Kanda F., Hirao A., Egi M., Noguchi A., Hashimoto S., Aya U., Sakuramoto H., Ohuchi A., Kataoka J., Maruyama K., Nakayama I., Nishime Y., Fujimoto K., Takahashi K., Tsujimoto M., Shimizu M., Tole E., Correia M.C., Kim J.H., Park S., Kim K.C., Baek J., Bae J.-M., Park S.Y., Park T.S., Lee H.B., Park J., Yeon-Joo L., Young-Jae C., Jeon K., Kim S.C., Lee J., Chee H.K., Huh J.W., Sim Y.S., Kim J., Chang Y., Ahn J.-J., Kang B.J., Lee W.-Y., Lee S.J., Baftiu N., Krastins I., Stiban S., Feghaly M.E., Gharios E., Merheb M., Benlamin M., Khaled A., Belkhair W.A., Tabib M., Ashour F., Elhadi A., Tababa O.W.E., Khaled T., Alkhumsi S.I.R., Alshrif A.I., Aboufray A.A., Alabuzidi A., Triki A.R., Elgammudi M., Zahra H.B., Soula E., Al-Alawi M.M.S., Ahmed H., Ghula M.A.A., Vosylius S., Mouton L., Rastegar T., Sertznig C., Martin G., Theisen C., Ferretti C., Gils F., Gallion M., Zainudin A., Bahrin L.K.K., Deva S.R., Rahim A.H.A., Wahab S., Hassan W.N.W., Ismail W.N.W., Ali M.N., Khoo T.M., Samat N.M., Tong J.M.G., Adib N.A.N., Nor M.B.M., Ismail N., Sulaiman S.R., Foong K.W., Alias A., Hua N.P., Zermeno J.M., Blanco D., Duran K., Nava C.L.L., Nandyelly S.J.R., Sanchez-Hurtado L.A., Tejeda-Huezo B., Del Moral Armengol M., Nava L.P.A., Herrera J.G., de Anda G.F.V., Gallegos-Perez H., Hernandez-Sanchez N., Hernandez-Ponce L., Gorordo-Delsol L., Hernandez-Romero M., Gomez S., Molinar F., Namendys-Silva S.A., Romero-Gonzalez J.P., Gonzalez D., Landaverde A., Sosa M.A., Navarro B., de Molina Serrano J.I.R., Iburrigarro S.R., Ibarra A., Aguirre J., Martinez-Gonzalez M., Padilla N.R.C., Pineda A.A.V., Villafuerte M.V.E., Herrera M.O.G., Baasanjav B., Hachimi A., Elkhayari M., Dendane T., Subedi N.B., Pathak S.D., Manandhar M., Van Gulik L., Van Den Brink M., Van Vliet P., Gerretsen B., Van Den Berg L., De Haan M., Tuinstra B., Kuijpers P., Reijntjens J., Vermeijden J.W., Rinket M., Vanroest M., Reidinga A., Loef B., Dieperink W., Onrust M., Dormans T., Bormans L., Koopmans M., Gerritsen R.T., Van Den Elst A., Evers M., Oiting O., Wilting R., Ramaker B., van der Kuil M., Fijen J.-W., Haas L., De Lange D., Haringman J., Newby L., Gilder E., Hacking D., Dagooc R., Song R., Waibel H., Dawn F., Rapley J., Chadwick L., Chapman C., Crone P., Albrett J., Marko P., Goodson J., Browne T., Whitticase R., Davidson C., Judd H., Owens D., Onyeka T., Ugwu I., Ilesanmi R., Adejumo P.O., Owojuyigbe A., Adenekan A., Uba S., Chime C., Jibrin D., Sankey B.J., Adekola O., Olanipekun S., Shosolcheva M., Gievski V., Kartalov A., Naumovski F., Kuzmanovska B., Trposak A., Bogoevska-Miteva Z., Rosalia R., Olsen B.F., Sjobo B., Jensen K.D., Sykehus D., Johansen B.F., Straede E., Johansen E., Finnstrom I.J., Toellefsen A., Ostenjo H., Bjorgen H., Bratsberg B., Kristoffersen E., Skorstad E.M., Hansen S., Vullum S., Lunde G.A., Arntsen W., Lund M., Akselsen G.R., Monstad K.R., Stenset A., Haugom H., Monsen B., Hogvall L., Trudvang S., Galaaen B., Malmin S.K., Andersen M.H., Hargott R.F., Andersen Y., Steffenak E., Nyhus M., Meland B., Hashmi M., Rivas N., Maidana E., de Jesus Ortiz A., Cabral D.M.B., Simi M., Aponte C., Rivas J.C., Gill S., Garcia A., Alvarenga G., Cespedes L., Perez H., Moreira M.L., Canete F., Gonzalez R., Monges N., Coman M., Pederzani M., Franco N., Aganon F., Martinez R., Noblezada-Uy D., Ellazar C.G., Cerezo F.D., Palo J.E., Aperocho C.A.J., Isanan M., Tubacka M., Jasiewicz P., Czuczwar M., Borys M., Gutysz-Wojnicka A., Glinka L., Gawda R., Bilawicz J., Cabrita P., Vieira J., Figueiredo M.F., Pinheiro C.M., Antunes N., Pedro L., Ferreira F., Parente I., Varela M., Fernandes F., Martins C., Viveiros A., Cavaco R., Rita C.S., Dias S., Feranandes A.M., Silva P., Nunes C., Cabral J., Pires F., Ferreira H., Santos J., Pinto V.M.V., Bispo B.M., Ferreira A., Molinos E., Lafuente E., Gregorio R., Costa H., Lima A., Ferreira S., Seromenho V., Luis E., Valerio I., Cesar H., Tavares A., Alsheikhly A.S., Mahmood S., Guran C.T., Moise A., Filipescu D.C., Luchian M., Popescu M., Scutariu M.A., Petrisor C., Hagau N., Grigoras I., Patrichi T., Gusarev V., Pivkina A., Kulakov V., Ignatenko O., Kovaleva J., Zhivotneva T., Zhedaeva M., Matiushkov N., Ershova O., Egorova N., Khoronenko V., Baskakov D., Sergeev D., Piradov M., Grishina L., Magomedov M., Zuev E., Gorokhovatsky U., Leonova A., Fadeeva L., Belskiy V., Galishevskiy D., Zubareva N., Tribulev M., Zueva O., Kiselev A., Kamenshchikov N., Tokareva E., Petrushin M., Starchenko I., Nshimyumuremyi I., Muhizi J., Buregeya E., Nzarora J., Assiri A., Ebaid M.S., Almekhlafi G., Mandourah Y., Velickovic J., Velickovic D., Jovanovic B., Hadzibegovic A., Stefanovic B., Misic V., Bumbasirevic V., Rajkovic M., Stojanovic M., Gavrilovic S., Stanojevic M., Yaghi A., Turcan A., Firment P., Rabarova D., Lancaricova D., Vlaovic J., Groznik M., Lukic M., Perme J., Sostaric M., Umek N., Mirkovic T., Dolenc S., Fister M., Zorko N., Markota A., Yeni N.P., Jali P., Schmollgruber S., Syed M.R., Parag N., Wise R., Galiana M., Navarro J.A., De Pablo A.M., Albert P., Martinez P., Mendiara Y., Garcia B., Llinas A.A., Riveiro M., Gallart E., Riera A., Sanz M., Salo S., Lajara M.A.G., Nieto M.V., Garcia R., Pena J.M.G., Gorgolas M.C., Isasi M.A., Sierra R., Gordo F., Conejo I., Salva-Costa V., Garzon-Tovar C., Lospitao S., Gutierrez P., Girona M., Adamuz J., Olivares P.G., de Ceballos J.P.G., Tirado C., De Wit I., Polo A.B.C., del Mar Diaz Salcedo M., Ripolles-Melchor J., Martinez-Hurtado E., Alvarez J.D., Arcas M.L.B., Gonzalez J.I.T., de la Ventana A.B.S., Calleja P.L.-A., Alvarez R.G., Zamora P.S., Guerrero A.O., Cosano R., Perez-Vacas J., Campos-Perez M., Barreiro E.M., Sanchez L.C., Diaz M.G., Jimenez R., Del Rio Cabajo L., Muriel D.S., Alonso H.F., Fernandez A.W., Pinan I.S., Albaiceta G.M., Fernandez M.C.I., Abos F.J.S., Monedero P., Chueca R.M., Aguirre L.G., Manosa S.C., Luque C.P., Calpe N., Losilla M.R., Fores M.T., Farre O., Fernandez O., del Rosario Villar Redondo M., Arteta Arteta D.S., Sanchez M.A.H., Espinosa C.P., Reyes L.M., Domenech L.C., Guillen C.V., Alvarez J.T., del Cotillo M., Barrueco-Francioni J.E., Conde B.B., Blanco M.P.S., Blasco M.L., Clement A.I., Hurtado C., Sanz L.C., Perez-Torres D., Prol-Silva E., Pereira J., Gonzalez I.A., Cano A.E., Nunez C.R., Fernadez I.L., Fernandez A.A.M., Del Bosque Diez R., Hilario B., Zalba-Etayo B., Pascual-Bielsa A., Banwarie P., Nahar D., van Axel A., Boedjawan N.N., Jansson E.B., Malvemyr A.-S., Johansson L., Sandberg U., Tingsvik C., Mattsson G., Lof G., Spangfors M., Ringdal M., Geijer S., Orvelius L., Hylen M., Lagerhall C., Akerman E., Hellkvist V.H., Mickelsson U., Wahlbom E., Larsson I.-M., Wallin E., Boroli F., Ory S., Jong M.L., Dullenkopf A., Lang M., Fleury Y., Maus M., Ben-Hamouda N., Fishman A., Hsu M.Y., Chang S.C., Trongtratul K., Sawawiboon C., Morakul S., Khwannimit B., Singh K., Ventour D., Figaro-Barclay D., Sankar-Maharaj S., Mebazaa M.S., Kamoun S., Elatrous S., Besbes L., Abroug F., Naija W., Elhechmi Y.Z., Sellami W., Hajjej Z., Merhabene T., Talik I., Kuscu O.O., Dilek O., Zerman A., Dal H.C., Turan S., Aydemir S., Yilmaz H., Calili D.K., Izdes S., Cengiz M., Gumus A., Tasdemir B., Kagnici A., Ay M., Ay S.A., Caliskan G., Akbas T., Balbay A.O., Efe S., Inal V., Elay G., Karabacak P., Ozserezli B., Senturk E., Demirkiran O., Bozbay S., Erdogan E., Akker M., Peker N., Ozgultekin A., Serin S.O., Turan C., Karaoren G., Goksu S., Karakurt S., Arikan H., Gul F., Cinel I., Kara I., Undar H.N., Bayraktar Y.S., Celik J.B., Tokur M.E., Aydin D.T., Yildiz I., Ozcan B., Erdivanli B., Eroglu A., Akdag D., Unlu N., Dungca A., Ali A., Thankamma B., Reyes P.E., John S., Rajendran A., Ahmad F.K.E., Smiley K.A., Hojden S., Miller M.T., Das Sasidharan Nair V., Antonio M.G.S., Qawasmeh K.A., Shawish S.A., Twiggs H., Rosado I., Babych V., Morren F., Young C., Vaughan-Jones N., Harris S., Burns K., Georgiev C., Shayamano R., Kerslake I., Creber P., Vochin A., O'Brien C., Caddell P., Hagan S., Hughes M., Torlinski T., Sherwin J., Kannan S., Markham A., Lebon R., Cupitt J., Cranshaw J., White N., Marriott V., Milner W., Groba C.B., Azoia J., Polgarova P., George S., Kapoor R., Lynch C., Fox N., Cranmer K., Llewellym T., Matthews K., Maltby L., Ibao J., Boulton K., Jarman R., Baxter K., Raj A.S., Moghal A., White J., Barrowcliffe S., Pulletz M., Ganeshalingam V., Baruah R., Baker H., Woods J., Ei P.P., Ogbeide V., Hayden P., Hughes J., Balasubramanian M., Salberg A., Saha R., Holmquist D., Derbyshire C., Smith N., Stones E., Ademokun J., Legorburo M.S., North S., Brett C., Jaundoo H., Craig J., Whiteley S., Howcroft C., Wilby L., Delve P., Shaw D., Williams K., Welters I.D., McMullen J., Brett S., Flores L., Trueman-Dawkins T., Templeton M., Adams J., Prowle J., Byers H., McDonnell A., Rose B.O., Reece-Anthony R., Mendes L., Vizcaychipi M., Bull R., Lacaden G., Santiago E., Delgado C.C., Farnell-Ward S., Thorpe E., Somerville J., Williams A., Cummings D., Derrick H., Brumwell S., Randell C., McCann N., Aves E., Berry G., Szakmany T., Gunter U., Pulak P., Sarkar N., Wright K., Gomes V., Jones J., Palfrey R., Camsooksai J., Lewis A., Eneas A., Tridente A., Barr L., Thomas B., Parkin E., Horner D., Frey C., Bench S., Baumber R., Broadhurst P., Jackson M., Williams L., Clark M., Paddle J., Bean S., Buckley S., Palfreeman C., Liu S., Allison N., Attwood B., Parsons P., Houghton V., Turner S.J., Higgins D., Bielskute E., Horrigan N., Jacob R., Habgood K., Zaki A., Collins A., Lord J., Ramiro C., Kubisz-Pudelko A., Kotze M., Williams H., Iovenko I., Tsarev A., Briva A., Mendez G., Napolitano L., Teig M., Rodriguez G.E., Ben-Jacob T., Potestio C., Eng T., Mahanes D., Khanna A., Duggal A., Nananmori M., Lois M., Karamchandani K., Bealer C., Barefield C., Terry D., Fivecoat P., Idowu O., Cata J., Clesi T., Peterson J., Hatton K., Dhaliwal J., Mueller D., Tao J., Eltorai A.S., Pastores S.M., Remor N., Salazar J., Barkas D., Joffe A., Barnes C., Sona C., Schallom M., Short J., Lorenzo J., Von Der Osten I., Borkowska M., Demarre L., Pleitinckx V., Xing C., Debue A.-S., Goller S., Larina E., Labeau, S. O., Blackwood, B., Brett, S. J., Deschepper, M., Francois, G., Honore, P. M., Khanna, A. K., Williams, G., Blot, S. I., Ribas, A. M., Telleria, V. M., Nogueira, P. C., Nafees, K. M. K., Reyes, A. G., Jensen, H. I., Adipa, F. E., Agoston, Z., Hashemian, S. M., Lee, S. -M., Abillama, F. F., Nor, B. M., Mazlan, M. Z., Sanchez-Hurtado, L., De lange, D., Ilesanmi, R. E., Ffarcsi, M. H., Hernandez, A. M., Schefold, J. C., Besbes, L. O., Minope, J. T. S., Rostello, O. F., Bartoli, J. R., Nocheretti, M. G., Escalante, R. G., Loudet, C. I., Gonzalez, A. L., Alvarez, G. A., Campos, P. A., Fonseca, I. P., Alvarez, G. M., Bascary, C. A., del Valle Gimenez, G., Bertoletti, F. P., Bonsignore, P. J. M., Fernandez, M. A., Leslie, G. D., Mclucas, A., Jacquet, L. -M., de Almeida, A. O., Jorge, S. A., Schmidt, R. C., Garcia, P. C., Ronchini, A. L. V., Manap, N. B. A., Laizner, A. M., Mcquirter, M., Kampayana, B. S., Sepulveda, M. I., Zamorano, M. J. F., Zhao, R. H., Hu, L. P., Jiao, Q. F., Wang, H. Y., Xia, C. J., Insu, L., Zhu, J. Y., Zhu, J. F., Huang, R. F., Wang, L. L., Song, J. H., Liu, X. M., Li, Z. S., Li, L. C., Zeng, J. M., Hu, X. C., Wang, R. X., Tak, P. S., Ho, S. W., Jiang, Q. X., Huang, L. P., Liu, X. L., Jiang, J. H., Gong, Y. Y., Lei, D. H., Bi, A. P., Zhao, H. M., Cao, Z. Q., Wu, S. F., Tian, X. F., Feng, Z. X., Liu, X. Z., Jiang, Z. X., Wang, G. X., Hu, R. L., Li, X. Q., Yu, Z. J., Yang, Y. X., Gama, L. M. S., Hernandez, J. S., Ochoa, M. -E., Reyes, A. J. G., Filipovic-Grcic, I., Vukovic, A., Pecenkovic, S., Suput, A., Radivojevic, R. C., Culjak, H., Adam, V. N., Pedersen, K. R., Kjaergard, I. E., Kodal, A. M., Hansen, T. C. B., Pedersen, A. S. B., Thomsen, T. D., Frandsen, T. M., Bliksted, I. A., Tamayo, L. M., Tutillo, D. R. M., Hurtado, C. V., Garcia, M. F., Kutimets, M., Lofqvist, C., Sakki, J. -K., Valta, M. A., Plantefeve, G., Deserts, M. D., Gunther, S. C., Timsit, J. -F., Farkas, J. -C., Bosl, K., Schuppel, S., Stubner, A., Osei, I. P., Kusi-Appiah, A. -C., Yakubu, Y. H., Patsiou, E. -C., Stalika, K. M. M., Enamorado, J. E., Jonasdottir, R. J., Lestari, M. I., Finn, D. O. C. R., Mcpherson, S., Ghioldi, D. M., Bruno, A. V., Maggiore, S. M., Volta, C. A., Taibi, M. R., Tranello, F. P., Giusti, G. D., Martin, M. A., Correia, M. C., Kim, J. H., Kim, K. C., Bae, J. -M., Park, S. Y., Park, T. S., Lee, H. B., Kim, S. C., Chee, H. K., Huh, J. W., Sim, Y. S., Ahn, J. -J., Kang, B. J., Lee, W. -Y., Lee, S. J., Feghaly, M. E., Belkhair, W. A., Tababa, O. W. E., Alkhumsi, S. I. R., Alshrif, A. I., Aboufray, A. A., Triki, A. R., Zahra, H. B., Al-Alawi, M. M. S., Ghula, M. A. A., Bahrin, L. K. K., Deva, S. R., Rahim, A. H. A., Hassan, W. N. W., Ismail, W. N. W., Ali, M. N., Khoo, T. M., Samat, N. M., Tong, J. M. G., Adib, N. A. N., Nor, M. B. M., Sulaiman, S. R., Foong, K. W., Hua, N. P., Zermeno, J. M., Nava, C. L. L., Nandyelly, S. J. R., Sanchez-Hurtado, L. A., Nava, L. P. A., Herrera, J. G., de Anda, G. F. V., Namendys-Silva, S. A., Romero-Gonzalez, J. P., Sosa, M. A., de Molina Serrano, J. I. R., Iburrigarro, S. R., Padilla, N. R. C., Pineda, A. A. V., Villafuerte, M. V. E., Herrera, M. O. G., Subedi, N. B., Pathak, S. D., Vermeijden, J. W., Gerritsen, R. T., Fijen, J. -W., Adejumo, P. O., Sankey, B. J., Olsen, B. F., Jensen, K. D., Johansen, B. F., Finnstrom, I. J., Skorstad, E. M., Lunde, G. A., Akselsen, G. R., Monstad, K. R., Hogvall, L., Malmin, S. K., Andersen, M. H., Hargott, R. F., de Jesus Ortiz, A., Cabral, D. M. B., Rivas, J. C., Moreira, M. L., Ellazar, C. G., Cerezo, F. D., Palo, J. E., Aperocho, C. A. J., Figueiredo, M. F., Pinheiro, C. M., Rita, C. S., Feranandes, A. M., Pinto, V. M. V., Bispo, B. M., Lima, A., Alsheikhly, A. S., Guran, C. T., Filipescu, D. C., Scutariu, M. A., Ebaid, M. S., Velickovic, D., Rajkovic, M., Stanojevic, M., Turcan, A., Lancaricova, D., Yeni, N. P., Syed, M. R., Navarro, J. A., De Pablo, A. M., Llinas, A. A., Lajara, M. A. G., Nieto, M. V., Pena, J. M. G., Gorgolas, M. C., Isasi, M. A., Salva-Costa, V., Garzon-Tovar, C., Olivares, P. G., de Ceballos, J. P. G., Polo, A. B. C., del Mar Diaz Salcedo, M., Alvarez, J. D., Arcas, M. L. B., Gonzalez, J. I. T., de la Ventana, A. B. S., Calleja, P. L. -A., Alvarez, R. G., Zamora, P. S., Guerrero, A. O., Barreiro, E. M., Sanchez, L. C., Diaz, M. G., Muriel, D. S., Alonso, H. F., Fernandez, A. W., Pinan, I. S., Albaiceta, G. M., Fernandez, M. C. I., Abos, F. J. S., Chueca, R. M., Aguirre, L. G., Manosa, S. C., Luque, C. P., Losilla, M. R., Fores, M. T., del Rosario Villar Redondo, M., Arteta Arteta, D. S., Sanchez, M. A. H., Espinosa, C. P., Reyes, L. M., Domenech, L. C., Guillen, C. V., Alvarez, J. T., del Cotillo, M., Barrueco-Francioni, J. E., Conde, B. B., Blanco, M. P. S., Blasco, M. L., Clement, A. I., Sanz, L. C., Gonzalez, I. A., Cano, A. E., Nunez, C. R., Fernadez, I. L., Fernandez, A. A. M., Boedjawan, N. N., Jansson, E. B., Malvemyr, A. -S., Lof, G., Spangfors, M., Lagerhall, C., Akerman, E., Hellkvist, V. H., Larsson, I. -M., Jong, M. L., Hsu, M. Y., Chang, S. C., Mebazaa, M. S., Elhechmi, Y. Z., Kuscu, O. O., Dal, H. C., Calili, D. K., Izdes, S., Gumus, A., Tasdemir, B., Kagnici, A., Ay, S. A., Balbay, A. O., Ozserezli, B., Senturk, E., Serin, S. O., Gul, F., Cinel, I., Undar, H. N., Bayraktar, Y. S., Celik, J. B., Tokur, M. E., Aydin, D. T., Yildiz, I., Ozcan, B., Akdag, D., Unlu, N., Reyes, P. E., Ahmad, F. K. E., Smiley, K. A., Miller, M. T., Antonio, M. G. S., Qawasmeh, K. A., Shawish, S. A., Groba, C. B., Raj, A. S., Ei, P. P., Legorburo, M. S., Welters, I. D., Mcmullen, J., Mcdonnell, A., Rose, B. O., Delgado, C. C., Mccann, N., Turner, S. J., Rodriguez, G. E., Eltorai, A. S., Pastores, S. M., Demarre, L., and Debue, A. -S.
Subjects: Male, Original, medicine.medical_treatment, artificial, Critical Care and Intensive Care Medicine, Medical and Health Sciences, Pressure ulcer, law.invention, Decubitus epidemiology, ICU, Morbidity, Mortality, Outcome, Pressure injury, Risk factors, Adult, Aged, Hospital Mortality, Humans, Patient Discharge, Prevalence, Risk Factors, Intensive Care Units, Respiration, Artificial, 0302 clinical medicine, decubitus epidemiology, pressure injury, pressure ulcer, outcome, risk factors, morbidity, mortality, law, Medicine and Health Sciences, adults, Medicine, Simplified Acute Physiology Score, icu, ziekenhuissterfte, Immunodeficiency, intensive care, European Society of Intensive Care Medicine (ESICM) Trials Group Collaborators, mannen, volwassenen, COST, Intensive care unit, STATE, ULCERS, Underweight, medicine.symptom, Life Sciences & Biomedicine, Human, medicine.medical_specialty, risicofactoren, Decubitus epidemiology, ICU, Pressure injury, Pressure ulcer, Outcome, Risk factors, Morbidity, Mortality, pressure injuries, Intensive Care Unit, prevalentie, NO, 1117 Public Health and Health Services, DecubICUs Study Team, 03 medical and health sciences, Critical Care Medicine, Anesthesiology, General & Internal Medicine, Health Sciences, ouderen, Mechanical ventilation, Science & Technology, business.industry, decubitus, Risk Factor, 030208 emergency & critical care medicine, 1103 Clinical Sciences, Odds ratio, medicine.disease, Emergency & Critical Care Medicine, Confidence interval, 030228 respiratory system, Emergency medicine, kunstmatige ademhaling, RISK-FACTORS, business, respiration
Abstract: Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347, Funder: Flemish Society for Critical Care Nurses, Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score < 19, ICU stay > 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat.
Published: 2021
Full Text: View/download PDF

12. Effectiveness of Silicon Platelet-Rich Fibrin and Autologous Bone on Bone Regeneration in Rabbit Calvarian Defects: A Radiological and Histological Study

Author: Argimiro Hernández-Suarez, María Rizo-Gorrita, Dubraska Suárez-Vega, Gladys Velazco, Ivan Rodriguez Gelfenstein, Celia Vázquez-Pachón, María-Ángeles Serrera-Figallo, and Daniel Torres-Lagares
Subjects: Technology, Silicon, QH301-705.5, QC1-999, chemistry.chemical_element, Dentistry, platelet-rich fibrin, 02 engineering and technology, Fibrin, 03 medical and health sciences, 0302 clinical medicine, bone regeneration, Cranial vault, Medicine, General Materials Science, Biology (General), Bone regeneration, QD1-999, Instrumentation, Fluid Flow and Transfer Processes, biology, Osteoid, business.industry, Physics, Process Chemistry and Technology, Regeneration (biology), General Engineering, silicon, 030206 dentistry, Engineering (General). Civil engineering (General), 021001 nanoscience & nanotechnology, Autologous bone, Platelet-rich fibrin, Computer Science Applications, Chemistry, chemistry, biology.protein, TA1-2040, 0210 nano-technology, business, PRF, autologous bone
Abstract: Repairing bone defects in oral surgery often requires the use of bone regeneration techniques. Silicon is an element that has been employed as regeneration material in several studies. In our study, silicon was combined with autologous bone and platelet-rich fibrin (PRF) membranes to analyse the behaviour of this element in bone regeneration. Four circumferential defects were created in the cranial vault of five New Zealand rabbits. The following elements were applied to the regeneration of the defects: (P): PRF, (S): silicon and (B): autologous bone, with the following distribution of study groups: Group 1 (PSB), Group 2 (PS), Group 3 (SB) and Group 4 (CONTROL): unregenerate group. The animals were sacrificed after 3 weeks. Computed microtomography studies (μ-CT) were carried out, as well as histomorphometric ones. The ANOVA statistical test was used with a Bonferroni post-hoc test to compare the results (p ≤ 0.05). Radiologically, groups PSB and SB were better as far as quantity and percentage of healthy bone observed, but not significantly compared to the control group. The PS group was significantly worse. The histological test revealed that the PSB group was the one to present the largest area, percentage and perimeter of mineralised bone. On evaluating the forming bone (osteoid), no difference was observed across the groups with the exception of the bone perimeter, where the SB group was significantly better. The bone height variable showed no significant differences. In conclusion we can state that the combination of PRF, autologous bone and silicon provides good results at 3 weeks whilst the PS group shows the worst results. This highlights the importance of autologous bone forming part of the graft material in order for the bone to mineralise.
Published: 2021
Full Text: View/download PDF

13. Current evidence for lung ultrasound elastography in the field of pneumology: a systematic review

Author: Fernando Vargas-Ursúa, Cristina Ramos-Hernández, Luis Alberto Pazos-Area, Ignacio Fernández-Granda, Iván Rodríguez-Otero, Eva Gómez-Corredoira, Manuel Pintos-Louro, and Alberto Fernández-Villar
Subjects: Medicine
Abstract: Background and objectives Elastography is a technology that has strongly impacted several medical specialties; however, it is not yet applied as part of standard clinical practice in the field of pulmonology. The objective of this systematic review is to analyse the evidence available to date in relation to pleuropulmonary ultrasound elastography, focusing on the three pathologies with the most publications: subpleural consolidations, interstitial lung diseases and pleural effusion. Methods Original in vivo studies published up until 12 August 2023 in the Embase, MEDLINE or Web of Science databases were included. The QUADAS-2 tool was applied to analyse bias. Results We found 613 records in database search. After duplicates removal, we screened 246 records and finally included 18 papers. The average cohort sample size was 109 patients. The elastography modes most frequently used were strain (22.2%), transient elastography (22.2%), point shear-wave elastography (38.9%) and two-dimensional shear-wave elastography (22.2%). The possibility of a meta-analysis was ruled out because of the heterogeneity of the studies included. Discussion The currently available literature indicates that pleuropulmonary ultrasound elastography produces promising and consistent results, although the lack of standardisation in the use of the technique and in the elastography modes employed still impedes its use in daily clinical pneumology practice. The development of a clinical guideline establishing a common nomenclature and standardised techniques for pleuropulmonary elastography will be imperative to generate quality scientific evidence in this field.
Published: 2024
Full Text: View/download PDF

14. Telerehabilitación respiratoria en fibrosis quística: experiencia de pacientes, padres y profesionales durante la pandemia de COVID-19. Estudio cualitativo.

Author: Iván Rodríguez Núñez, María José Bretti, Ana María Daroch Neira, Daniel Zenteno Araos, and Gerardo Torres Puebla
Subjects: fibrosis quística, rehabilitación respiratoria, telerehabilitación, barreras, facilitadores, investigación cualitativa, Medicine
Abstract: Introducción: la rehabilitación respiratoria (RR) se recomienda en pacientes con fibrosis quística (FQ). Durante la pandemia de COVID-19 los programas de RR debieron cerrarse o migrar a modalidades de telerehabilitación, imponiendo nuevos desafíos a pacientes y equipos de salud. El objetivo de este estudio fue explorar las percepciones de pacientes, padres y profesionales sobre la transición a la telerehabilitación respiratoria durante la pandemia de COVID-19. Método: estudio cualitativo. Se consideraron pacientes con FQ mayores de 8 años. También a padres y equipos de salud. El tamaño muestral se determinó mediante saturación teórica. Se realizaron entrevistas semiestructuradas y grupos focales vía Zoom ®. El análisis de datos se realizó mediante los métodos de codificación abierta y axial. El análisis se realizó utilizando el software Atlas. Ti 7.5.7. Resultados: se incluyó a 4 pacientes adultos, 1 pediátrico y 2 padres, además de 4 profesionales de equipos de salud. Existió una percepción general positiva respecto a la RR y la telerehabilitación. Entre las barreras destacó la falta de equipamiento para la telerehabilitación en domicilio y la organización diaria de los pacientes. Entre los facilitadores destacó la disponibilidad de equipos y redes que permitieran la conectividad y el apoyo familiar. Existió una valoración positiva hacia la continuidad de la telerehabilitación en la etapa post pandémica. Conclusiones: la telerehabilitación fue percibida como una alternativa viable y efectiva, sin embargo, aspectos de la conectividad, disponibilidad de equipamiento y la rutina diaria de los pacientes debe ser considerada a la hora de implementar modalidades telemáticas de atención.
Published: 2024
Full Text: View/download PDF

15. Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets

Author: Michael S. Cohen, David Hutin, Jason Matthews, Larry L. David, Ilsa T Kirby, Marit Rasmussen, Kelsie M Rodriguez, Ivan Rodriguez Siordia, Denis M. Grant, Sara C. Buch-Larsen, and Michael L. Nielsen
Subjects: Proteome, QH301-705.5, Science, Regulator, Chemical biology, Nucleoside Transport Proteins, General Biochemistry, Genetics and Molecular Biology, PARPs, proteomics, ADP-Ribosylation, Biochemistry and Chemical Biology, Humans, Cysteine, Biology (General), Polymerase, Zinc finger, Innate immune system, General Immunology and Microbiology, biology, Chemistry, General Neuroscience, Chromosome Mapping, RNA-Binding Proteins, General Medicine, Cell biology, chemical genetics, click chemistry, biology.protein, Medicine, Chemical genetics, Research Article, proximity labeling, Human
Abstract: Poly(ADP-ribose) polymerase 7 (PARP-7) has emerged as a critically important member of a large enzyme family that catalyzes ADP-ribosylation in mammalian cells. PARP-7 is a critical regulator of the innate immune response. What remains unclear is the mechanism by which PARP-7 regulates this process, namely because the protein targets of PARP-7 mono-ADP-ribosylation (MARylation) are largely unknown. Here, we combine chemical genetics, proximity labeling, and proteome-wide amino acid ADP-ribosylation site profiling for identifying the direct targets and sites of PARP-7-mediated MARylation in a cellular context. We found that the inactive PARP family member, PARP-13—a critical regulator of the antiviral innate immune response—is a major target of PARP-7. PARP-13 is preferentially MARylated on cysteine residues in its RNA binding zinc finger domain. Proteome-wide ADP-ribosylation analysis reveals cysteine as a major MARylation acceptor of PARP-7. This study provides insight into PARP-7 targeting and MARylation site preference.
Published: 2021

16. Unfavorable hydroxychloroquine COVID-19 research associated with authors having a history of political party donations

Author: Bruce B. Berry, Andrew C. Berry, Ivan Rodriguez, Qianying Zhang, and Russell S. Gonnering
Subjects: political party, 2019-20 coronavirus outbreak, lcsh:Diseases of the circulatory (Cardiovascular) system, Coronavirus disease 2019 (COVID-19), cardiac, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), political bias, chloroquine, Politics, Antimalarials, medicine, Humans, donations, business.industry, Outcome measures, Gift giving, Hydroxychloroquine, General Medicine, Publication bias, Gift Giving, United States, COVID-19 Drug Treatment, sars-cov-2, covid-19, Financial Disclosures, lcsh:RC666-701, Donation, Cardiology and Cardiovascular Medicine, business, Psychology, Publication Bias, Demography, medicine.drug
Abstract: OBJECTIVE: To explore the degree to which political bias in medicine and study authors could explain the stark variation in Hydroxychloroquine (HCQ)/Chloroquine (CQ) study favorability in the US compared to the rest of the world. SETTING: United States and Worldwide. PARTICIPANTS: COVID-19/SARS-CoV-2 preprint and published papers between January 1, 2020 and July 26, 2020 with the terms Hydroxychloroquine and/or Chloroquine; 267 met study criteria, of which 68 originated from the US. 68 control studies were randomly selected from the same time period to serve as a control subset for baseline COVID-19 publication trends and author characteristics, not related to HCQ/CQ. MAIN OUTCOME MEASURES: HCQ/CQ study result favorability, with each recorded as favorable, unfavorable, or neutral. First and last main authors of each US study were entered into FollowTheMoney.org Website, to extract any history of political party donation, and to which political party. RESULTS: Of all US studies (68 total), 39/68 (57.4%) were unfavorable, with only 7/68 (10.3%) of US studies yielding favorable results. This was compared to 199 non-US studies, in with 66/199 (33.2%) were unfavorable, 69/199 (34.7%) favorable, and 64/199 (32.2%) neutral. Studies with at least one US main author were 20.4% (SE 0.053, P
Published: 2020

17. SARS-CoV-2 receptors and entry genes are expressed in the human olfactory neuroepithelium and brain

Author: Basile Nicolas Landis, Joël Tuberosa, Ivan Rodriguez, Kristof Egervari, Alan Carleton, Daniel Rossier, Veronique Pauli, Johannes Alexander Lobrinus, Leon Fodoulian, Chenda Kan, and Madlaina Boillat
Subjects: 0301 basic medicine, Bioinformatics, media_common.quotation_subject, Anosmia, Omics, Sensory system, 02 engineering and technology, ddc:616.07, Biology, Microbiology, Virus, Article, 03 medical and health sciences, ddc:590, Virology, medicine, lcsh:Science, Internalization, Receptor, Enhancer, media_common, Multidisciplinary, Virus receptor, Cell Biology, Biological Sciences, 021001 nanoscience & nanotechnology, ddc:616.8, Olfactory bulb, Cell biology, 030104 developmental biology, lcsh:Q, medicine.symptom, 0210 nano-technology, hormones, hormone substitutes, and hormone antagonists
Abstract: Reports indicate an association between COVID-19 and anosmia, as well as the presence of SARS-CoV-2 virions in the olfactory bulb. To test whether the olfactory neuroepithelium may represent a target of the virus, we generated RNA-seq libraries from human olfactory neuroepithelia, in which we found substantial expression of the genes coding for the virus receptor angiotensin-converting enzyme-2 (ACE2), and for the virus internalization enhancer TMPRSS2. We analyzed a human olfactory single-cell RNA-seq dataset and determined that sustentacular cells, which maintain the integrity of olfactory sensory neurons, express ACE2 and TMPRSS2. ACE2 protein was highly expressed in a subset of sustentacular cells in human and mouse olfactory tissues. Finally, we found ACE2 transcripts in specific brain cell types, both in mice and humans. Sustentacular cells thus represent a potential entry door for SARS-CoV-2 in a neuronal sensory system that is in direct connection with the brain., Graphical Abstract, Highlights • SARS-CoV-2 receptors ACE2 and TMPRSS2 are expressed in olfactory neuroepithelia • ACE2 and TMPRSS2 are coexpressed in supporting sustentacular cells • A subset of neuronal and non-neuronal cells in the brain transcribe ACE2
Published: 2020

18. The claustrum-medial prefrontal cortex network controls attentional set-shifting

Author: Roberta Leone, Rodrigo F. Salazar, Kazadi Ekundayo, Jean-Rodolphe Renfer, Chieko Huber, Alan Carleton, Sophie Mutel, Leon Fodoulian, Ivan Rodriguez, and Olivier Gschwend
Subjects: 0303 health sciences, integumentary system, food and beverages, Cognition, Claustrum, Attentional set, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, nervous system, Cortex (anatomy), medicine, Excitatory postsynaptic potential, lipids (amino acids, peptides, and proteins), Psychology, Prefrontal cortex, Neuroscience, Nucleus, psychological phenomena and processes, 030217 neurology & neurosurgery, 030304 developmental biology
Abstract: SUMMARYIn various mental disorders, dysfunction of the prefrontal cortex contributes to cognitive deficits. Here we studied how the claustrum (CLA), a nucleus sharing reciprocal connections with the cortex, may participate in these cognitive impairments. We show that specific ensembles of CLA and of medial prefrontal cortex (mPFC) neurons are activated during a task requiring cognitive control such as attentional set-shifting, i.e. the ability to shift attention towards newly relevant stimulus-reward associations while disengaging from irrelevant ones. CLA neurons exert a direct excitatory input on mPFC pyramidal cells, and chemogenetic inhibition of CLA neurons suppresses the formation of specific mPFC assemblies during attentional set-shifting. Furthermore, impairing the recruitment of specific CLA assemblies through opto/chemogenetic manipulations prevents attentional set-shifting. In conclusion, we propose that the CLA controls the reorganization of mPFC ensembles to enable attentional set-shifting, emphasizing a potential role of the CLA-mPFC network in attentional dysfunctions.
Published: 2020

19. Omega 3 Fatty Acids and COVID-19: A Comprehensive Review

Author: Susan Manella, Sarabjot Singh-Makkar, Adrian Riva-Moscoso, Rockeven Desir, Rafael Abreu, Zar Chi Min, Muhammad Khawar Sana, Aayushi Patel, Madhusudan Patel, Donald Hathaway, Krunal Pandav, Rafael Sanchez-Dopazo, Michael Maher Mourad Fahem, Bishnu Mohan Singh, Alina Alvarez, and Ivan Rodriguez
Subjects: Eicosapentaenoic acid, Review Article, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Omega-3 fatty acids, Pharmacology (medical), 030212 general & internal medicine, Disseminated intravascular coagulation, 0303 health sciences, 030306 microbiology, business.industry, SARS-CoV-2, Mortality rate, COVID-19, Immune dysregulation, medicine.disease, Docosahexaenoic acid, Infectious Diseases, Immunology, Cytokine storm, Multiple organ dysfunction syndrome, business
Abstract: The rapid international spread of severe acute respiratory syndrome coronavirus 2 responsible for coronavirus disease 2019 (COVID-19) has posed a global health emergency in 2020. It has affected over 52 million people and led to over 1.29 million deaths worldwide, as of November 13th, 2020. Patients diagnosed with COVID-19 present with symptoms ranging from none to severe and include fever, shortness of breath, dry cough, anosmia, and gastrointestinal abnormalities. Severe complications are largely due to overdrive of the host immune system leading to "cytokine storm". This results in disseminated intravascular coagulation, acute respiratory distress syndrome, multiple organ dysfunction syndrome, and death. Due to its highly infectious nature and concerning mortality rate, every effort has been focused on prevention and creating new medications or repurposing old treatment options to ameliorate the suffering of COVID-19 patients including the immune dysregulation. Omega-3 fatty acids are known to be incorporated throughout the body into the bi-phospholipid layer of the cell membrane leading to the production of less pro-inflammatory mediators compared to other fatty acids that are more prevalent in the Western diet. In this article, the benefits of omega-3 fatty acids, especially eicosapentaenoic acid and docosahexaenoic acid, including their anti-inflammatory, immunomodulating, and possible antiviral effects have been discussed.
Published: 2020

20. SARS-CoV-2 receptor and entry genes are expressed by sustentacular cells in the human olfactory neuroepithelium

Author: Madlaina Boillat, Veronique Pauli, Basile Nicolas Landis, Kristof Egervari, Leon Fodoulian, Joël Tuberosa, Ivan Rodriguez, Daniel Rossier, Johannes Alexander Lobrinus, Alan Carleton, and Chenda Kan
Subjects: Neuroepithelial cell, medicine.anatomical_structure, Viral entry, medicine, Anosmia, Priming (immunology), Sensory system, Biology, medicine.symptom, Receptor, Virus, Respiratory tract, Cell biology
Abstract: The recent emergence of the pathogenic SARS-CoV-2 initiated a worldwide health crisis. The entry of the virus into cells is mediated by the binding of the viral Spike protein to the angiotensin-converting enzyme-2 (ACE2), followed by its priming by the TMPRSS2 serine protease, both present on the cellular membrane of the target cells. In the respiratory tract, these targets are ciliated cells. Interestingly, various reports indicate an association between SARS-CoV-2 infection and anosmia, suggesting an alteration not restricted to the respiratory tissue, but that might also include the olfactory sensory epithelium. We explored this possibility by generating RNA-seq libraries from human neuroepithelium, in which we found significant expression of ACE2 and TMPRSS2. To determine whether specific cell types of this chemosensory tissue may coexpress both of the virus entry genes, we analyzed a scRNA-seq dataset. We determined that sustentacular cells, which are in direct contact with the external world and maintain the integrity of olfactory sensory neurons, represents a prime candidate for SARS-CoV-2 infection via the nose, and possibly for SARS-CoV-2-induced anosmia.
Published: 2020

21. Benefits of the Therapy With Abexol in Patients With Non-Alcoholic Fatty Liver Disease

Author: Ivan Rodriguez-Perez, Julio César Fernández-Travieso, Caridad Ruenes-Domech, Jose lIlnait-Ferrer, Sarahí Mendoza-Castaño, and Lilia Fernández-Dorta
Subjects: 0301 basic medicine, medicine.medical_specialty, Cirrhosis, medicine.medical_treatment, Placebo, Gastroenterology, Abexol, 03 medical and health sciences, HOMA index, 0302 clinical medicine, Internal medicine, Insulin levels, medicine, Beeswax, business.industry, Non-alcoholic fatty deposition liver disease, Incidence (epidemiology), Insulin, Fatty liver, medicine.disease, Clinical trial, 030104 developmental biology, 030211 gastroenterology & hepatology, Original Article, Steatosis, Steatohepatitis, business
Abstract: Background Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of diseases ranging from steatosis to steatohepatitis and cirrhosis. Given the increasing incidence of NAFLD and the long-term consequences of this disease, it is important to identify the risk factors and therapeutic measures. Abexol is a mixture of beeswax alcohols with antioxidant, gastro-protective and anti-inflammatory effects. The aim was to conduct a pooled analysis of clinical trials data of the effects of Abexol treatment in patients with NAFLD. Methods The present analysis includes the data of all patients with NAFLD obtained from medium-term randomized, double-blinded, placebo controlled clinical studies with Abexol. One hundred patients with NAFLD received Abexol (100 mg/day) or placebo for 6 months. Significant changes in the ultrasound analysis of the liver were considered a primary efficacy variable. Secondary endpoints were decreased homeostasis model assessment (HOMA) index and insulin levels, and improved clinical symptoms. Statistical analysis of all data was according to the intention-to-treat method. Results Both groups were statistically homogeneous at baseline conditions. At 6 months of treatment, the number of Abexol-treated patients exhibiting a normal liver echo pattern on ultrasonography was greater than that of the placebo patients (P < 0.05). Abexol significantly reduced (P < 0.05) insulin levels and HOMA index. The proportion of Abexol patients showing symptom improvement was higher (P < 0.01) than that of the placebo group. Treatments were safe and well tolerated. Conclusions Treatment of Abexol during 6 months significantly ameliorates liver fat accumulation and insulin resistances, meanwhile improving clinical evolution in patients with NAFLD. The treatment was safe and well tolerated in these patients.
Published: 2020

22. Frequency of Gastro-Esophageal Reflux symptoms in Healthy Children Determined by MM GERD Questionnaire in Mexico: An Exploratory Study

Author: ez-Pliego, Jose Ivan Rodriguez de Molina-Serrano, and Rogelio Ramses Hern
Subjects: medicine.medical_specialty, business.industry, Exploratory research, Construct validity, Primary care, medicine.disease, Gastro esophageal reflux, Cronbach's alpha, Physical therapy, medicine, GERD, Upper gastrointestinal, business, Pediatric population
Abstract: Background: GERD is a common disorder in children representing an important cause of consultation. Questionnaires have been proposed as a useful tool for primary care medicine but also as a cost effective option. However, it ’ s use still controversial in pediatric population. In this exploratory study, we have used the MM GERD Questionnaire in 2 elementary schools. Objective: An exploratory study to evaluate the MM GERD questionnaire in children of 5-17 years old of 2 elementary schools in Mexico City. Methods: A prospective, descriptive exploratory study including male and female children 5-17 years in which parents or tutors accept and sign ICF and willing to answer the 4 scale multi-dimensional GERD scale symptom (10 items), pain intensity scale (3 items); disability scale (3 items) and satisfaction scale (2 items). Results: A total of 223 children were included with 123 male and 99 female with mean age of 8.64 ± 2.7, weight 32.94 ± 14.17 kg, height 1.30 ± 0.17 mt and BMI of 18.67 ± 4.9. There was good construct validity among 4 scales measured with Pearson coefficient. The inter-item consistency (Cronbach’s co-efficient alpha) for the symptoms items, pain intensity items, disability items and satisfaction items were 0.41, 0.78, 0.87 and 0.4, respectively, demonstrating adequate reliability of the measure. Conclusion: MM GERD is a not a reliable questionnaire to explore the presence of upper gastrointestinal symptoms in healthy children from 5-17 years old. Thus several limitations, it is necessary to assess language, methodological and clinical validation of MM GERD to extrapolate these results into clinical practice.
Published: 2020

23. Acute Myeloid Leukemia Cell-Intrinsic PD-1 Functions Promoting Leukemia Development By Recruiting SHP2

Author: Tinisha McDonald, Shanshan Suo, Ling Li, Dandan Zhao, Junjing Qiao, Fenglin Li, Chen Liang, Fang Chen, Guido Marcucci, Ivan Rodriguez, Lucy Ghoda, Jie Jin, Dinh Hoa Hoang, Le Xuan Truong Nguyen, and Bin Amber Zhang
Subjects: business.industry, Immunology, Cell, Myeloid leukemia, Cell Biology, Hematology, medicine.disease, Biochemistry, Leukemia, medicine.anatomical_structure, hemic and lymphatic diseases, Cancer research, Medicine, business
Abstract: Acute myeloid leukemia (AML) is a devastating hematopoietic malignancy. With current therapies, only approximately 30% of patients achieve long-term survival. Therefore, novel, more active and less toxic treatments are urgently needed. Programmed death-1 (PD-1) is a cell surface receptor that functions as a T cell checkpoint and plays a central role in regulating T cell exhaustion. Binding of PD-1 to its ligand, programmed death-ligand 1 (PD-L1), activates downstream signaling pathways and inhibits T cell activation. Abnormally high PD-L1 expression on tumor cells and antigen-presenting cells in the tumor microenvironment mediates tumor immune escape, and PD-1/PD-L1 immune checkpoint blockade has showed promising results in cancer patients. Recently, PD-1 expressed on melanoma cells was also shown to play a pivotal role in tumor growth. To date, in AML, the function of PD-1 has been mainly studied in the host T cells, while little is known regarding the role of PD-1 in AML cells. Herein, we examined the level and role of PD-1 in AML cells using AML murine model and patient samples. We used MLL PTD/WT/Flt3 ITD/ITD knock-in mouse in B6 background, a well characterized AML model, to study the expression and function of PD-1 in AML. Flow cytometric analysis of LSK (Lin -Sca-l1 +c-kit +) cells from the bone marrow (BM) of wild-type (WT, n=5) and AML (n=10) mice showed that 20.9%-61.9% of AML LSKs versus (vs) To assess the functional role of PD-1 in AML, we sorted PD-1 + and PD-1 - fractions fromAML LSKs and observed a lower frequency of quiescent cells (G0, 16.60% vs 44.87%, P< 0.05) and a higher cell growth rate in the PD-1 + vs PD-1 - cells. Further in vivo study showed that PD-1 + AML LSKs (CD45.2) generated higher white blood cell (WBC) counts (P< 0.0001), higher AML engraftment (P< 0.0001) and a shorter survival (median survival 57.5 vs >75 days, P< 0.001) in recipient mice (CD45.1) compared with PD-1 - AML LSKs. Similar results were observed in human samples. Compared to PD-1 - CD34 + cells, PD-1 + CD34 + cells are less quiescent and more proliferative (P< 0.01). PD-1 + AML blasts had higher engraftment rate (13.18% vs 2.68%, p=0.0002) and shorter survival (median survival: 27 vs 45 days, P= 0.0008) in NSGS mice than PD-1 - AML blasts. To evaluate if these in vivo differences observed in PD-1 + vs PD-1 - AML LSKs were mediated by interactions between PD-1 + AML and T cells, PD-1 + and PD-1 -LSKs from AML mouse were transplanted into T-, B- cell-deficient NSG mice. Recipient mice transplanted with PD-1 + AML LSKs had higher WBC counts (P< 0.01), higher AML engraftment (P< 0.0001) and a shorter survival (median survival: 76 vs >130 days, P< 0.0001) than recipients with PD-1 - AML LSKs, suggesting that these differences were T cell-independent. Next, we examined whether blocking PD-1 could affect leukemic cell growth. We sorted LSK cells from AML mice and performed colony forming cell (CFC) assay in the presence of anti-PD1 antibody or isotype antibody. Blocking PD-1 with anti-PD-1 antibody significantly suppressed CFC and cell growth in vitro but did not induce apoptosis compared to isotype control antibody. To explore the molecular mechanism by which PD-1 contributes to AML growth, we then sorted PD-1 + and PD-1 - LSKs from AML mice for molecular studies. Western blot assays revealed higher levels of SHP-2 and phosphorylated (p) -ERK in PD-1 + vs PD-1 - AML LSKs. We validated these results in primary human AML cells by immunofluorescence staining. Confocal microscopy of PD-1 + and PD-1 - human AML CD34 +cells demonstrated that PD-1 localized at the cell membrane and in the cytoplasm and p-ERK was markedly enhanced in the PD-1 + CD34 + cells. In conclusion, we showed here that a subpopulation of murine and human AML blasts expresses high levels of PD-1 which mediated disease initiation and growth through activation of the MAPK/ERK signaling pathways. PD-1 blocking antibody reversed these activities and might contribute to the clinical efficacy of anti-PD-1 therapy in AML. Disclosures Marcucci: Novartis: Other: Speaker and advisory scientific board meetings; Agios: Other: Speaker and advisory scientific board meetings; Abbvie: Other: Speaker and advisory scientific board meetings.
Published: 2021

24. Evolution of immune chemoreceptors into sensors of the outside world

Author: Gregory Loichot, Ivan Rodriguez, Joël Tuberosa, Alan Carleton, Manuel Ruedi, Lone N. Rösingh, and Quentin Dietschi
Subjects: 0301 basic medicine, Sensory Receptor Cells, Vomeronasal organ, Evolution, Neofunctionalization, Olfaction, Biology, Evolution, Molecular, Mice, 03 medical and health sciences, Immune system, ddc:590, Formyl peptide receptor, Gene expression, medicine, Animals, Humans, Promoter Regions, Genetic, Receptor, Gene, Phylogeny, Inflammation, Neurons, Genetics, Rodent, Multidisciplinary, Olfactory receptor, Gene Expression Profiling, Chemosensor, Exons, Biological Sciences, Receptors, Formyl Peptide, Chemoreceptor Cells, ddc:616.8, Rats, Cell biology, Smell, 030104 developmental biology, medicine.anatomical_structure, Immune System, Multigene Family, Vomeronasal Organ
Abstract: Changes in gene expression patterns represent an essential source of evolutionary innovation. A striking case of neofunctionalization is the acquisition of neuronal specificity by immune formyl peptide receptors (Fprs). In mammals, Fprs are expressed by immune cells, where they detect pathogenic and inflammatory chemical cues. In rodents, these receptors are also expressed by sensory neurons of the vomeronasal organ, an olfactory structure mediating innate avoidance behaviors. Here we show that two gene shuffling events led to two independent acquisitions of neuronal specificity by Fprs. The first event targeted the promoter of a V1R receptor gene. This was followed some 30 million years later by a second genomic accident targeting the promoter of a V2R gene. Finally, we show that expression of a vomeronasal Fpr can reverse back to the immune system under inflammatory conditions via the production of an intergenic transcript linking neuronal and immune Fpr genes. Thus, three hijackings of regulatory elements are sufficient to explain all aspects of the complex expression patterns acquired by a receptor family that switched from sensing pathogens inside the organism to sensing the outside world through the nose.
Published: 2017

25. Wolbachia Promotes an Anti-Angiogenic Response Using an In Vitro Model of Vascular Endothelial Cells in Relation to Heartworm Disease

Author: Manuel Collado-Cuadrado, Claudia Alarcón-Torrecillas, Iván Rodríguez-Escolar, Alfonso Balmori-de la Puente, Elena Infante González-Mohino, Miguel Pericacho, and Rodrigo Morchón
Subjects: anti-angiogenesis, Wolbachia sp., rWSP, Dirofilaria immitis, endothelial cells, Medicine
Abstract: Heartworm disease caused by Dirofilaria immitis is a vector-borne zoonotic disease responsible for the infection of mainly domestic dogs and cats, or these are those for which the most data are known. Humans are an accidental host where a benign, asymptomatic pulmonary nodule may originate. Dirofilaria immitis also harbours the endosymbiont bacteria of the genus Wolbachia, which play a role in moulting, embryogenesis, inflammatory pathology, and immune response. When Wolbachia sp. is released into the bloodstream, endothelial and pulmonary damage is exacerbated, further encouraging thrombus formation and pulmonary hypertension, facilitating congestive heart failure and death of the animal. Previous studies have shown that parasite excretory/secretory products are able to activate the pro-angiogenic pathway (formation of new vessels) to facilitate parasite survival. The aim of this study was to analyse the role of Wolbachia sp. and its relationship with the cellular processes and the angiogenic pathway in a model of human endothelial cells in vitro. The use of recombinant Wolbachia Surface Protein (rWSP) showed that its stimulation exerted an anti-angiogenic effect by detecting an increase in the production of VEGFR-1/sFlt1 and sEndoglin and did not affect the production of VEGFR-2 and mEndoglin (pro-angiogenic molecules). Furthermore, it did not stimulate cell proliferation or migration, although it did negatively stimulate the formation of pseudocapillaries, slowing down this process. These cellular processes are directly related to the angiogenic pathway so, with these results, we can conclude that Wolbachia sp. is related to the stimulation of the anti-angiogenic pathway, not facilitating the survival of D. immitis in vascular endothelium.
Published: 2024
Full Text: View/download PDF

26. Maximum swimming speeds of sailfish and three other large marine predatory fish species based on muscle contraction time and stride length: a myth revisited

Author: Alexander D. M. Wilson, P. E. Viblanc, Ralf H. J. M. Kurvers, John F. Steffensen, Jens Krause, Morten Bo Søndergaard Svendsen, Stefano Marras, Kevin M. Boswell, Jean S. Finger, Ivan Rodriguez-Pinto, and Paolo Domenici
Subjects: 0106 biological sciences, Maximum swimming speed, Little tunny, QH301-705.5, Science, 010603 evolutionary biology, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Istiophorus platypterus, Predatory fish, medicine, Barracuda, Biology (General), biology, Coryphaena hippurus, 010604 marine biology & hydrobiology, Pelagic zone, Stride length, Sailfish, biology.organism_classification, Black marlin, Fishery, Muscle twitch, Euthynnus alletteratus, medicine.symptom, Sphyraena barracuda, General Agricultural and Biological Sciences, Muscle contraction, Research Article
Abstract: Billfishes are considered to be among the fastest swimmers in the oceans. Previous studies have estimated maximum speed of sailfish and black marlin at around 35 m s−1 but theoretical work on cavitation predicts that such extreme speed is unlikely. Here we investigated maximum speed of sailfish, and three other large marine pelagic predatory fish species, by measuring the twitch contraction time of anaerobic swimming muscle. The highest estimated maximum swimming speeds were found in sailfish (8.3±1.4 m s−1), followed by barracuda (6.2±1.0 m s−1), little tunny (5.6±0.2 m s−1) and dorado (4.0±0.9 m s−1); although size-corrected performance was highest in little tunny and lowest in sailfish. Contrary to previously reported estimates, our results suggest that sailfish are incapable of exceeding swimming speeds of 10-15 m s−1, which corresponds to the speed at which cavitation is predicted to occur, with destructive consequences for fin tissues., Summary: Using muscle contraction measurements, this work provides evidence that sailfish are most likely unable to reach the extremely high speeds claimed by previous research and popular articles.
Published: 2016

27. Evaluating Motion and Heart Rate Sensors to Measure Intensity of Physical Activity

Author: Ivan Rodriguez, Pablo Pancardo, and Miguel A. Wister
Subjects: 020203 distributed computing, Computer science, Physical activity, Measure (physics), 030229 sport sciences, 02 engineering and technology, Overweight, Accelerometer, Motion (physics), Intensity (physics), 03 medical and health sciences, 0302 clinical medicine, Human–computer interaction, Heart rate, 0202 electrical engineering, electronic engineering, information engineering, medicine, medicine.symptom, Motion sensors
Abstract: Using a device for measuring the intensity of a physical activity when a person carries out their daily routines is an important support to monitor their health, especially if this person is overweight or obese since it exists risk for their health when demanding a lot of energy while performing physical activities. To confront this problem, there are new generation devices for measuring physical activity, that can be used to know physical intensity levels and consequently, establish exercise programs if were necessary to lose weight or maintain a certain level of training derived from a medical prescription. This paper evaluates the relationship between values of a motion sensor and heart rate sensor for measuring the intensity of physical activity of overweight or obese people. We propose to use these two sensors to determine the correlation between both so that at a given time, the motion sensor can be a useful alternative to measure the intensity of physical activity. This option makes easier for people to measure physical intensity with a conventional device equipped with an accelerometer, many people that use smartphones might avoid going to an expert to keep track of physical exercises.
Published: 2018

28. Neuronal pattern separation in the olfactory bulb improves odor discrimination learning

Author: Frédéric Begnaud, Samuel Lagier, Nixon M. Abraham, Olivier Gschwend, Ivan Rodriguez, and Alan Carleton
Subjects: Male, Olfactory system, Sensory processing, medicine.medical_treatment, Sensory system, Optogenetics, Article, Discrimination Learning, Mice, ddc:590, medicine, Animals, Learning, optogenetics, Behavior, Microscopy, Confocal, head-restrained behavior, General Neuroscience, Odor discrimination, Olfactory Pathways, in-vivo extracellular recording, Olfactory Perception, Immunohistochemistry, Olfactory Bulb, Olfaction, Olfactory stimulus, ddc:616.8, Olfactory bulb, Mice, Inbred C57BL, Chemogenetic, Population coding, odorant mixture, Pattern separation, GABAergic, Optogenetic, Psychology, Neuroscience
Abstract: Neuronal pattern separation is thought to enable the brain to disambiguate sensory stimuli with overlapping features, thereby extracting valuable information. In the olfactory system, it remains unknown whether pattern separation acts as a driving force for sensory discrimination and the learning thereof. We found that overlapping odor-evoked input patterns to the mouse olfactory bulb (OB) were dynamically reformatted in the network on the timescale of a single breath, giving rise to separated patterns of activity in an ensemble of output neurons, mitral/tufted (M/T) cells. Notably, the extent of pattern separation in M/T assemblies predicted behavioral discrimination performance during the learning phase. Furthermore, exciting or inhibiting GABAergic OB interneurons, using optogenetics or pharmacogenetics, altered pattern separation and thereby odor discrimination learning in a bidirectional way. In conclusion, we propose that the OB network can act as a pattern separator facilitating olfactory stimulus distinction, a process that is sculpted by synaptic inhibition.
Published: 2015

29. Sensory-Evoked Intrinsic Imaging Signals in the Olfactory Bulb Are Independent of Neurovascular Coupling

Author: Ivan Rodriguez, Samuel Lagier, Dimitri Van De Ville, Alan Carleton, and Roberto Vincis
Subjects: Genetically modified mouse, Cell type, Action potential, Mice, Transgenic, Sensory system, Biology, Olfactory Receptor Neurons, Article, General Biochemistry, Genetics and Molecular Biology, Mice, chemistry.chemical_compound, ddc:590, Evoked Potentials, Somatosensory, Olfactory Marker Protein, medicine, Animals, Premovement neuronal activity, Neurotransmitter, lcsh:QH301-705.5, Neurotransmitter Agents, Osmolar Concentration, Hemodynamics, Anatomy, Magnetic Resonance Imaging, Olfactory Bulb, Axons, Dynamic Light Scattering, Sensory neuron, ddc:616.8, Olfactory bulb, Mice, Inbred C57BL, medicine.anatomical_structure, lcsh:Biology (General), Microscopy, Fluorescence, chemistry, Functional brain imaging, Neurovascular Coupling, Female, Axon swelling, Neuroscience
Abstract: Summary Functional brain-imaging techniques used in humans and animals, such as functional MRI and intrinsic optical signal (IOS) imaging, are thought to largely rely on neurovascular coupling and hemodynamic responses. Here, taking advantage of the well-described micro-architecture of the mouse olfactory bulb, we dissected the nature of odor-evoked IOSs. Using in vivo pharmacology in transgenic mouse lines reporting activity in different cell types, we show that parenchymal IOSs are largely independent of neurotransmitter release and neurovascular coupling. Furthermore, our results suggest that odor-evoked parenchymal IOSs originate from changes in light scattering of olfactory sensory neuron axons, mostly due to water movement following action potential propagation. Our study sheds light on a direct correlate of neuronal activity, which may be used for large-scale functional brain imaging.
Published: 2015

30. Restoring wild type-like network dynamics and behaviour during adulthood in a mouse model of schizophrenia

Author: Dominique Muller, Sophie Mutel, Cristina Bertollini, Thomas Marissal, Rodrigo F. Salazar, Ivan Rodriguez, Mathias De Roo, and Alan Carleton
Subjects: 0303 health sciences, biology, Interneuron, Wild type, Hippocampal formation, Network dynamics, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, 22q11 Deletion Syndrome, Neurodevelopmental disorder, medicine.anatomical_structure, nervous system, Schizophrenia, mental disorders, biology.protein, medicine, Neuroscience, 030217 neurology & neurosurgery, Parvalbumin, 030304 developmental biology
Abstract: Schizophrenia is a severely debilitating neurodevelopmental disorder. Establishing a causal link between circuit dysfunction and particular behavioural traits relevant to schizophrenia is crucial to shed new light on the mechanisms underlying the pathology. Here we studied an animal model of the 22q11 deletion syndrome, which is the highest genetic risk to develop the pathology. We report a desynchronization of hippocampal neuronal assemblies that resulted from parvalbumin interneuron hypoexcitability. Rescuing parvalbumin interneuron excitability with pharmacological or chemogenetic approaches is sufficient to restore wild type-like network dynamics and behaviour during adulthood. In conclusion, our data provide mechanistic insights underlying network dysfunction relevant to schizophrenia and demonstrate the potential of reverse engineering in fostering new therapeutic strategies to alleviate the burden of neurodevelopmental disorders.
Published: 2017

31. American lobster Cathepsin D, an aspartic peptidase resistant to proteolysis and active in organic solvents, non-ionic detergents and salts

Author: María de Toro, Ivan Rodriguez-Siordia, Liliana Rojo-Arreola, and Fernando García-Carreño
Subjects: 0106 biological sciences, 0301 basic medicine, Glycerol, Protein Conformation, Proteolysis, Detergents, Cathepsin D, Sodium Chloride, 01 natural sciences, Biochemistry, Fluorescence, 03 medical and health sciences, chemistry.chemical_compound, Surface-Active Agents, Structural Biology, 010608 biotechnology, Enzyme Stability, Papain, Renin, medicine, Animals, Urea, Molecular Biology, Cathepsin, chemistry.chemical_classification, Ethanol, Chromatography, biology, medicine.diagnostic_test, General Medicine, Enzyme assay, Nephropidae, 030104 developmental biology, Enzyme, chemistry, biology.protein, Solvents, Salts
Abstract: Suitable peptidases for biotechnological applications are those active at low temperature, in organic solvents, detergents or proteolytic additives. American lobster cathepsin D1 (CD1) is an enzyme highly efficient at 5-50°C and at pH 2.5-5.5. We assessed the effect of common industrial additives on CD1 activity. CD1 was isolated from lobster gastric fluid by chromatography. The proteolytic activity was measured using a fluorogenic specific substrate and the conformation by intrinsic fluorescence. Non-ionic detergents Tween-20 and Triton X-100 stabilize the peptidase activity. Ethanol, methanol and isopropanol [5-15% (v/v)] increased the enzyme activity up to 80%. The enzyme is active until 2.5M urea and is resistant to proteolysis by papain and renin. In this work, a crustacean peptidase that remains active when exposed to different chemical and proteolytic additives is reported, evincing that crustaceans are a good model for discovery of novel stable peptidases for future pharmaceutical, cosmetic and alimentary applications.
Published: 2017

32. Elotuzumab in Combination with an Immunomodulatory Agent (IMID) in Patients with Multiple Myeloma Refractory to Imids and/or Daratumumab: A Single-Institution Analysis

Author: Elizabeth Finley-Oliver, Melissa Alsina, Jamie Shapiro, Ariel F Grajales-Cruz, Kenneth H. Shain, Omar Alexis Castaneda Puglianini, Cristian Ivan Rodriguez Arocho, Rachid Baz, Jason Brayer, Leonel Ochoa, Taiga Nishihori, and Ashley Rose
Subjects: Oncology, medicine.medical_specialty, business.industry, Immunology, Daratumumab, Cell Biology, Hematology, medicine.disease, Pomalidomide, Biochemistry, Refractory, Internal medicine, medicine, In patient, Immunomodulatory Agent, Elotuzumab, business, health care economics and organizations, Multiple myeloma, Lenalidomide, medicine.drug
Abstract: Background: Elotuzumab (elo), an immunostimulatory monoclonal antibody targeting signaling lymphocytic activation molecule F7 (SLAMF7, CD319) has shown activity in combination with dexamethasone and lenalidomide (len) or pomalidomide (pom), in patients with relapsed/refractory (RR) multiple myeloma (MM) in the ELOQUENT 2 and 3 trials, respectively. However, patients enrolled on those trials were not refractory to len or pom respectively and the impact of prior daratumumab (dara) (which can deplete natural killer (NK) cells) was not evaluated. We reviewed the outcomes of RR MM patients treated with elo in combination with either len or pom with respect to prior exposure and refractoriness to IMIDs and dara. Methods: We retrospectively evaluated patients with RR MM who were treated at Moffitt Cancer Center between January, 2017 and July, 2019 with elo in combination with len (elo/len) or pom (elo/pom). We assessed prior therapies and refractoriness to IMIDs and dara. Response was evaluated using the International Myeloma Working Group (IMWG) criteria with added minimal response. Grade 3/4 toxicities were reviewed. This study was approved by the Institutional Review Board. Results: Thirty six patients were identified: 14 on elo/len and 22 on elo/pom. Patient characteristics are outlined in the table. As expected, patients that received elo/pom were more heavily pre-treated, than those who received elo/len, with a median number of 6 prior lines of therapy. The median number of cycles was 9 (range, 2-33) and 3 (range, 1-8) for the elo/len and elo/pom groups, respectively. 21/22 (95%) patients in the elo/pom pts were pom-refractory, whereas 11/14 (79%) of the elo/len patients were len-refractory. For elo/len, the overall response rate (ORR) was 42%, and the median progression-free survival (PFS) was 7.7 months (95% confidence interval, 1.99-10.95, p=0.026) although the ORR was 28.6% in len refractory patients. For elo/pom, the ORR was 14% (for pom refractory patients the ORR was 9.1%) and the median PFS 3.7 months (95% confidence interval, 1.27-6.13 p=0.026). Twenty-three patients (64%) were refractory to dara, with a median time from dara to elo of 13.7 (range, 0.7-33.6) months. Overall survival (OS) and PFS based on the median time from dara to elo were not statistically different. The same analysis based on a 6 months cutoff (assuming shorter dara impact on NK cells) yielded similar results. Thirteen patients remain on treatment at the time of this analysis. Reasons for treatment discontinuation were progressive disease (PD) in 22 (95.7%), adverse event (AE) in 1 (4.3%). Grade 3/4 toxicities were noted in two patients; one patient developed pneumonitis, and another developed infectious complication. Conclusion: The combination of elo with an IMID, such as len or pom, is well tolerated. Elo/IMIDs regimen have modest efficacy in len and pom refractory patients respectively. The timing of and prior dara does not appear to impact the efficacy of elo based therapy. Prospective studies are needed to confirm these findings. Disclosures Shain: Adaptive Biotechnologies: Consultancy; AbbVie: Research Funding; Amgen: Membership on an entity's Board of Directors or advisory committees; Celgene: Membership on an entity's Board of Directors or advisory committees; Bristol-Myers Squibb: Membership on an entity's Board of Directors or advisory committees; Takeda: Membership on an entity's Board of Directors or advisory committees; Sanofi Genzyme: Membership on an entity's Board of Directors or advisory committees; Janssen: Membership on an entity's Board of Directors or advisory committees. Brayer:Janssen: Consultancy, Speakers Bureau; BMS: Consultancy, Speakers Bureau. Nishihori:Novartis: Research Funding; Karyopharm: Research Funding. Alsina:Bristol-Myers Squibb: Research Funding; Janssen: Speakers Bureau; Amgen: Speakers Bureau. Baz:AbbVie: Research Funding; Merck: Research Funding; Sanofi: Research Funding; Bristol-Myers Squibb: Research Funding; Celgene: Membership on an entity's Board of Directors or advisory committees, Research Funding; Karyopharm: Membership on an entity's Board of Directors or advisory committees, Research Funding.
Published: 2019

33. Clinical Relevance of the Fusion Transcripts Distribution Pattern in Mexican Children With Acute Lymphoblastic Leukemia

Author: Isidoro Tejocote-Romero, Manuel Martínez-Mancilla, Rodolfo Ocadiz-Delgado, Ivan Rodriguez-Aguirre, Aurora Medina-Sanson, and Patricio Gariglio
Subjects: Male, Oncology, medicine.medical_specialty, Pathology, Adolescent, Oncogene Proteins, Fusion, Context (language use), Real-Time Polymerase Chain Reaction, Immunophenotyping, Fusion gene, Internal medicine, Chromosomes, Human, Humans, Medicine, Clinical significance, Prospective Studies, Leukocytosis, Child, Prospective cohort study, Childhood Acute Lymphoblastic Leukemia, Homeodomain Proteins, business.industry, Infant, DNA, Neoplasm, Hematology, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Prognosis, Human genetics, Child, Preschool, Pediatrics, Perinatology and Child Health, Female, medicine.symptom, business
Abstract: Chromosomal translocation-generated fusion genes in childhood acute lymphoblastic leukemia (ALL) are well-known indicators of prognostic outcome. This study was conducted to establish the clinical relevance of the fusion genes distribution pattern in Mexican children with newly diagnosed ALL. Multiplex RT-PCR assays were used to detect 4 commonest fusion transcripts in 261 Mexican children with B-cell precursor ALL aged 1 to 14 years old, comparing differences in the distribution of the patients between molecular subgroups to a common collection of clinical parameters. We documented a 13% significant proportion of all patients who are more than 10 years of age, harboring fusion transcripts associated with leukocytosis and poor response to remission-induction chemotherapy, than those negative children for chimeric transcripts (P
Published: 2013

34. Dense encoding of natural odorants by ensembles of sparsely activated neurons in the olfactory bulb

Author: Olivier Gschwend, Ivan Rodriguez, Roberto Vincis, Jonathan Beroud, and Alan Carleton
Subjects: 0301 basic medicine, Male, Sensory Receptor Cells, Speech recognition, T cell, Population, Sensory system, Receptors, Odorant, Article, Tonic (physiology), 03 medical and health sciences, Mice, 0302 clinical medicine, Olfactory bulb, ddc:590, medicine, Animals, education, Tetrode (biology), Evoked Potentials, education.field_of_study, Multidisciplinary, Chemistry, Coding, musculoskeletal, neural, and ocular physiology, Olfactory Perception, Olfactory Bulb, ddc:616.8, 030104 developmental biology, medicine.anatomical_structure, Rate change, Odorants, Biophysics, Cell assembly, 030217 neurology & neurosurgery, psychological phenomena and processes
Abstract: Sensory information undergoes substantial transformation along sensory pathways, usually encompassing sparsening of activity. In the olfactory bulb, though natural odorants evoke dense glomerular input maps, mitral and tufted (M/T) cells tuning is considered to be sparse because of highly odor-specific firing rate change. However, experiments used to draw this conclusion were either based on recordings performed in anesthetized preparations or used monomolecular odorants presented at arbitrary concentrations. In this study, we evaluated the lifetime and population sparseness evoked by natural odorants by capturing spike temporal patterning of neuronal assemblies instead of individual M/T tonic activity. Using functional imaging and tetrode recordings in awake mice, we show that natural odorants at their native concentrations are encoded by broad assemblies of M/T cells. While reducing odorant concentrations, we observed a reduced number of activated glomeruli representations and consequently a narrowing of M/T tuning curves. We conclude that natural odorants at their native concentrations recruit M/T cells with phasic rather than tonic activity. When encoding odorants in assemblies, M/T cells carry information about a vast number of odorants (lifetime sparseness). In addition, each natural odorant activates a broad M/T cell assembly (population sparseness).
Published: 2016

35. Analysis of risk factors in children with asthma exacerbations treated at the National Institute of Respiratory Diseases 'Ismael Cosio Villegas' (INER)

Author: Myrna Raquel Ibarra Gomez, Claudia Garrido Galindo, Rogelio Ramses Hernandez Pliego, Angel Isaac Gomez Jacome, and Jose Ivan Rodriguez De Molina
Subjects: Pediatrics, medicine.medical_specialty, Inhalation, Exacerbation, business.industry, Respiratory disease, Emergency department, medicine.disease, Comorbidity, Atopy, medicine, Salbutamol, business, medicine.drug, Asthma
Abstract: Introduction: In Mexico it is one of the 10 leading causes of utilization of health services it is estimated that more than 5 million Mexicans suffer from asthma 20 . OBJECTIVE: Analyze exacerbations and associated factors that can help to identify patients at high risk. METHODS: Retrospective clinical essay of patients aged 1 to 15 years treated at the emergency department of pediatric pulmonology, diagnosed with asthma exacerbations and requiring hospitalization. Demographic, physical, birth related background, respiratory disease severity, exacerbation severity and socioeconomic factors, level of asthma control were described. RESULTS: 271 patients were reviewed (September 2013 to December 2014), the male dominance 1.25: 1, a median age of 8 years, history of atopy was 72%.The variables that showed an inverse correlation were: time tracking INER (0.007, -0.28 Coef, OR 0.74.), Correct technique of using inhaled medication (0.04, -0.98 Coef, OR 0.37.).In 142 patients (52%) didn´t use salbutamol for treatment of early symptoms of exacerbation. Only 26% were compliance with treatment, and only 28% used properly the inhalation device. Correlations with the primary outcome (exacerbation) the most significant correlation was adherence to treatment (Coef. -0.11), Correct technique in use inhaled medication (Coef. -0.14). CONCLUSIONS: The most common comorbidity was allergic rhinitis in 83% of cases. Of patients with allergic rhinitis, only 4.4% had treatment.Although only the Moderate-Severe Persistent stadium showed a statistically significant but weak correlation for asthma exacerbations.
Published: 2016

36. Increased brain damage after ischaemic stroke in mice lacking the chemokine receptor CCR5

Author: Michel Dubois-Dauphin, Stéphanie Julien, N. Marq-Lin, Silvia Sorce, Karl-Heinz Krause, Jérôme Bonnefont, and Ivan Rodriguez
Subjects: Pharmacology, education.field_of_study, Pathology, medicine.medical_specialty, biology, Chemokine receptor CCR5, business.industry, Population, Brain damage, medicine.disease, Brain ischemia, Cerebral circulation, medicine.artery, Middle cerebral artery, Occlusion, Immunology, medicine, biology.protein, medicine.symptom, business, education, Stroke
Abstract: Background and purpose: The chemokine receptor CCR5 is well known for its function in immune cells; however, it is also expressed in the brain, where its specific role remains to be elucidated. Because genetic factors may influence the risk of developing cerebral ischaemia or affect its clinical outcome, we have analysed the role of CCR5 in experimental stroke. Experimental approach: Permanent cerebral ischaemia was performed by occlusion of the middle cerebral artery in wild-type and CCR5-deficient mice. Locomotor behaviour, infarct size and histochemical alterations were analysed at different time points after occlusion. Key results: The cerebral vasculature was comparable in wild-type and CCR5-deficient mice. However, the size of the infarct and the motor deficits after occlusion were markedly increased in CCR5-deficient mice as compared with wild type. No differences between wild-type and CCR5-deficient mice were elicited by occlusion with respect to the morphology and abundance of astrocytes and microglia. Seven days after occlusion the majority of CCR5-deficient mice displayed neutrophil invasion in the infarct region, which was not observed in wild type. As compared with wild type, the infarct regions of CCR5-deficient mice were characterized by increased neuronal death. Conclusions and implications: Lack of CCR5 increased the severity of brain injury following occlusion of the middle cerebral artery. This is of particular interest with respect to the relatively frequent occurrence of CCR5 deficiency in the human population (1‐2% of the Caucasian population) and the advent of CCR5 inhibitors as novel drugs. British Journal of Pharmacology (2010) 160, 311‐321; doi:10.1111/j.1476-5381.2010.00697.x
Published: 2010

37. Formyl peptide receptor-like proteins are a novel family of vomeronasal chemosensors

Author: Ivan Rodriguez, Stéphane Rivière, Daniela Fluegge, Marc Spehr, and Ludivine Challet
Subjects: Olfactory system, Vomeronasal organ, Olfaction, Biology, Ligands, Sensory receptor, Olfactory Receptor Neurons, Cell Line, Mice, Vomeronasal receptor, medicine, Animals, Humans, Disease, Calcium Signaling, Inflammation, Genetics, Multidisciplinary, Formyl peptide receptor, Olfactory receptor, Gene Expression Profiling, Dendrites, Olfactory Perception, Receptors, Formyl Peptide, Cell biology, Smell, medicine.anatomical_structure, Organ Specificity, Pheromone, Vomeronasal Organ
Abstract: To date, four types of olfactory receptors have been described in mammals — odorant receptors, trace amine-associated receptors and vomeronasal type 1 and type 2 receptor superfamilies. Riviere et al. now report the identification of a additional novel olfactory receptor family, expressed by mouse vomeronasal sensory neurons. This family is encoded by five members of the formyl peptide receptor-related gene family (FPRs), which are otherwise known to mediate immune cell response to infection Cells expressing FPRs respond to ligands associated with disease and inflammation, which are excreted in urine, raising the possibility that FPRs detect the health status of individuals. Two different G-protein-coupled receptor families are known to mediate pheromonal cues in the mammalian vomeronasal organ. Here, members of a third family of receptors, the formyl peptide receptor-related gene family (FPRs), are shown to be expressed in the vomeronasal epithelium, with those cells expressing FPRs responding to ligands associated with disease and inflammation. This raises the possibility that FPRs detect the health status of individuals. Mammals rely heavily on olfaction to interact adequately with each other and with their environment1. They make use of seven-transmembrane G-protein-coupled receptors to identify odorants and pheromones. These receptors are present on dendrites of olfactory sensory neurons located in the main olfactory or vomeronasal sensory epithelia, and pertain to the odorant2, trace amine-associated receptor3 and vomeronasal type 1 (ref. 4) or 2 (refs 5–7) receptor superfamilies. Whether these four sensor classes represent the complete olfactory molecular repertoire used by mammals to make sense of the outside world is unknown. Here we report the expression of formyl peptide receptor-related genes by vomeronasal sensory neurons, in multiple mammalian species. Similar to the four known olfactory receptor gene classes, these genes encode seven-transmembrane proteins, and are characterized by monogenic transcription and a punctate expression pattern in the sensory neuroepithelium. In vitro expression of mouse formyl peptide receptor-like 1, 3, 4, 6 and 7 provides sensitivity to disease/inflammation-related ligands. Establishing an in situ approach that combines whole-mount vomeronasal preparations with dendritic calcium imaging in the intact neuroepithelium, we show neuronal responses to the same molecules, which therefore represent a new class of vomeronasal agonists. Taken together, these results suggest that formyl peptide receptor-like proteins have an olfactory function associated with the identification of pathogens, or of pathogenic states.
Published: 2009

38. Gene cluster lock after pheromone receptor gene choice

Author: Chenda Kan, Christian Lüscher, Marie-Christine Broillet, Daniele Roppolo, Sarah Vollery, and Ivan Rodriguez
Subjects: Male, Chemoreceptor Cells/metabolism, Vomeronasal Organ/cytology/metabolism, Pair-rule gene, Multigene Family/ genetics, Biology, Article, General Biochemistry, Genetics and Molecular Biology, Mice, Gene cluster, medicine, Animals, 5-HT5A receptor, Neurons, Afferent, Allele, Molecular Biology, Gene, Alleles, Genetics, Regulation of gene expression, Chemotactic Factors/genetics, Olfactory receptor, Chemotactic Factors, General Immunology and Microbiology, General Neuroscience, Null allele, Chemoreceptor Cells, Receptors, Pheromone, ddc:616.8, medicine.anatomical_structure, Gene Expression Regulation, Multigene Family, Receptors, Pheromone/ genetics, Neurons, Afferent/cytology/metabolism, sense organs, Vomeronasal Organ, Gene Deletion
Abstract: In mammals, perception of pheromones is based on the expression in each vomeronasal sensory neuron of a limited set of receptor genes, chosen among a large repertoire. Here, we report an extremely tight control of the monogenic and monoallelic transcription of the V1rb2 receptor gene. Combining genetic and electrophysiological approaches, we show that the transcription of a non-functional V1r allele leads to the coexpression of another, functional V1r gene. The choice of this coexpressed gene surprisingly includes genes located on the cluster homologous to the one from which the mutant allele is transcribed. However, V1r genes located in cis relative to the transcribed mutant allele are excluded from the coexpression choice. Our observations strongly suggest a monogenic regulatory mechanism acting (a) at a general level, via the expression of the V1r receptor itself, and (b) at a more local level, defined by the V1r gene cluster.
Published: 2007

39. Adenylyl cyclase-dependent axonal targeting in the olfactory system

Author: Daniel R. Storm, Julien A. Dal Col, Tomohiko Matsuo, and Ivan Rodriguez
Subjects: Olfactory system, medicine.medical_specialty, Sensory system, Olfaction, Biology, Cyclase, Gene Expression Regulation, Enzymologic, Adenylyl cyclase, Mice, chemistry.chemical_compound, Internal medicine, medicine, Animals, Molecular Biology, Mice, Knockout, Gene Expression Regulation, Developmental, Olfactory Pathways, Axons, Neuropilin-1, Sensory neuron, Olfactory bulb, Mice, Inbred C57BL, Endocrinology, medicine.anatomical_structure, nervous system, chemistry, Neuroscience, Transduction (physiology), Adenylyl Cyclases, Developmental Biology
Abstract: The vertebrate olfactory bulb is a remarkably organized neuronal structure,in which hundreds of functionally different sensory inputs are organized into a highly stereotyped topographical map. How this wiring is achieved is not yet understood. Here, we show that the olfactory bulb topographical map is modified in adenylyl cyclase 3 (adenylate cyclase 3)-deficient mice. In these mutants, axonal projection targets corresponding to specific odorant receptors are disorganized, are no longer exclusively innervated by functionally identical axonal projections and shift dramatically along the anteroposterior axis of the olfactory bulb. Moreover, the cyclase depletion leads to the prevention of neuropilin 1 (Nrp1) expression in olfactory sensory neuron axonal projections. Taken together, our data point to a major role played by a crucial element of the odorant-induced transduction cascade, adenylyl cyclase 3, in the targeting of olfactory sensory neuron axons towards the brain. This mechanism probably involves the regulation of receptor genes known to be crucial in axonal guidance processes.
Published: 2007

40. Pheromone receptors in mammals

Author: Ivan Rodriguez
Subjects: Male, Primates, Vomeronasal organ, Offspring, Biology, Receptors, Odorant, Evolution, Molecular, Mice, Behavioral Neuroscience, Endocrinology, medicine, Animals, Humans, Animal communication, Neurons, Afferent, Gene, Mammals, Communication, Olfactory receptor, Endocrine and Autonomic Systems, business.industry, Receptors, Pheromone, Smell, medicine.anatomical_structure, Gene Expression Regulation, Evolutionary biology, Sex pheromone, Pheromone, Female, Identification (biology), Vomeronasal Organ, business
Abstract: In most mammals, pheromone perception mediates intraspecies interactions related to reproduction, such as mate recognition, intermale aggressive behaviors, or exchanges between females and their offspring. Recent molecular findings, particularly the identification of two large pheromone receptor gene superfamilies, provide today invaluable tools to better understand the way mammals make sense of pheromonal information.
Published: 2004

41. Inclusion in the university: Who assumes responsibility? A qualitative study.

Author: María José Solis-Grant, María José Bretti-López, Camila Espinoza-Parçet, Cristhian Pérez-Villalobos, Iván Rodríguez-Núñez, Cristian Pincheira-Martínez, and Cristóbal Sepúlveda-Carrasco
Subjects: Medicine, Science
Abstract: Society challenges higher education institutions and their members to generate inclusive communities to enable the full development of all members. This study aims to analyze who is responsible for generating inclusion according to community members from a traditional Chilean University. We carried out qualitative research based on the Grounded Theory. We collected data through focus group and semi-structured Interviews, involving 14 undergraduate students, two post-graduate students, 17 faculty members, five non-teaching staff members, and nine executives officers. All of thembelonging to the three campuses of the University. We analyzed data using ATLAS.ti 7.5.7, using the constant comparison method and reaching an axial codification level. From the data analysis, 25 subcategories emerged, grouped into six categories. Later we organized them under the codification paradigm. Results highlighted the perception of the interaction and influence of the social, institutional, and personal fields in the inclusion phenomenon. Also, that inclusive practices must be a responsibility shared among different educational community members.
Published: 2023
Full Text: View/download PDF

42. Physiological characterization of formyl peptide receptor expressing cells in the mouse vomeronasal organ

Author: Ivan Rodriguez, Tobias Ackels, BenoÃ®t von der Weid, and Marc Spehr
Subjects: Yellow fluorescent protein, Genetically modified mouse, Vomeronasal organ, vomeronasal receptor, Neuroscience (miscellaneous), VNO, Olfaction, lcsh:RC321-571, lcsh:QM1-695, Cellular and Molecular Neuroscience, Vomeronasal receptor, medicine, ddc:610, Original Research Article, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, formyl peptide receptor, Formyl peptide receptor, biology, lcsh:Human anatomy, 3. Good health, sensory neurons, medicine.anatomical_structure, Genetically Engineered Mouse, biology.protein, vomeronasal organ, Neuron, Anatomy, Neuroscience, olfaction
Abstract: The mouse vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Based on largely monogenic expression of either type 1 or 2 vomeronasal receptors (V1Rs / V2Rs) or members of the formyl peptide receptor (FPR) family, the vomeronasal sensory epithelium harbors at least three neuronal subpopulations. While various neurophysiological properties of both V1R- and V2R-expressing neurons have been described using genetically engineered mouse models, the basic biophysical characteristics of the more recently identified FPR-expressing vomeronasal neurons have not been studied. Here, we employ a transgenic mouse strain that coexpresses an enhanced variant of yellow fluorescent protein together with FPR-rs3 allowing to identify and analyze FPR-rs3-expressing neurons in acute VNO tissue slices. Single neuron electrophysiological recordings allow comparative characterization of the biophysical properties inherent to a prototypical member of the FPR-expressing subpopulation of VNO neurons. In this study, we provide an in-depth analysis of both passive and active membrane properties, including detailed characterization of several types of voltage-activated conductances and action potential discharge patterns, in fluorescently labeled versus unmarked vomeronasal neurons. Our results reveal striking similarities in the basic (electro)physiological architecture of both transgene-expressing and non-expressing neurons, confirming the suitability of this genetically engineered mouse model for future studies addressing more specialized issues in vomeronasal FPR neurobiology.
Published: 2014

43. The Chemical MUPpeteer

Author: Ivan Rodriguez
Subjects: Vomeronasal organ, Major urinary proteins, Aggression, Ecology, Biochemistry, Genetics and Molecular Biology(all), Sex pheromone, medicine, Sensory system, medicine.symptom, Biology, Neuroscience, Article, General Biochemistry, Genetics and Molecular Biology
Abstract: Potential predators emit uncharacterized chemosignals that warn receiving species of danger. Neurons that sense these stimuli remain unknown. Here we show that detection and processing of fear-evoking odors emitted from cat, rat, and snake require the function of sensory neurons in the vomeronasal organ. To investigate the molecular nature of the sensory cues emitted by predators, we isolated the salient ligands from two species using a combination of innate behavioral assays in naïve receiving animals, calcium imaging, and cFos induction. Surprisingly, the defensive behavior-promoting activity released by other animals is encoded by species-specific ligands belonging to the major urinary protein (Mup) family, homologs of aggression-promoting mouse pheromones. We show that recombinant Mup proteins are sufficient to activate sensory neurons and initiate defensive behavior similar to native odors. This co-option of existing sensory mechanisms provides a molecular solution to the difficult problem of evolving a variety of species-specific molecular detectors.
Published: 2010
Full Text: View/download PDF

44. Mice cloned from embryonic stem cells

Author: Ivan Rodriguez, Ryuzo Yanagimachi, Peter Mombaerts, Teruhiko Wakayama, and Anthony C.F. Perry
Subjects: Cloning, Nuclear Transfer Techniques, Multidisciplinary, Genotype, Microinjections, Somatic cell, Cloning, Organism, Stem Cells, Clone (cell biology), Embryo, Biological Sciences, Biology, Embryo, Mammalian, Molecular biology, Embryonic stem cell, Chromosomes, Mice, medicine.anatomical_structure, Cell culture, Reproduction, Asexual, Oocytes, medicine, Animals, Blastocyst, Stem cell
Abstract: Cloning allows the asexual reproduction of selected individuals such that the offspring have an essentially identical nuclear genome. Cloning by nuclear transfer thus far has been reported only with freshly isolated cells and cells from primary cultures. We previously reported a method of cloning mice from adult somatic cells after nuclear transfer by microinjection. Here, we apply this method to clone mice from widely available, established embryonic stem (ES) cell lines at late passage. With the ES cell line R1, 29% of reconstructed oocytes developed in vitro to the morula/blastocyst stage, and 8% of these embryos developed to live-born pups when transferred to surrogate mothers. We thus cloned 26 mice from R1 cells. Nuclei from the ES cell line E14 also were shown to direct development to term. We present evidence that the nuclei of ES cells at G 1 - or G 2 /M-phases are efficiently able to support full development. Our findings demonstrate that late-passage ES cells can be used to produce viable cloned mice and provide a link between the technologies of ES cells and animal cloning. It thus may be possible to clone from a single cell a large number of individuals over an extended period.
Published: 1999

45. Variable Patterns of Axonal Projections of Sensory Neurons in the Mouse Vomeronasal System

Author: Paul Feinstein, Ivan Rodriguez, and Peter Mombaerts
Subjects: Olfactory system, Heterozygote, Vomeronasal organ, Green Fluorescent Proteins, Grueneberg ganglion, Gene Expression, Sensory system, Cell Count, Biology, General Biochemistry, Genetics and Molecular Biology, Olfactory Receptor Neurons, Vomeronasal receptor, Mice, medicine, Animals, Alleles, Germ-Line Mutation, DNA Primers, Base Sequence, Biochemistry, Genetics and Molecular Biology(all), Homozygote, Gene targeting, Anatomy, Axons, Chemoreceptor Cells, Cell biology, Rats, Luminescent Proteins, medicine.anatomical_structure, Lac Operon, Gene Targeting, Mutagenesis, Site-Directed, Pheromone, Neuron, Vomeronasal Organ
Abstract: The vomeronasal system mediates pheromonal effects in mammals. We have employed gene targeting technology to introduce mutations in a putative pheromone receptor gene, VRi2, in the germline of mice. By generating alleles differentially tagged with the histological markers taulacZ and tauGFP, we show that VRi2 is monoallelically expressed in a given neuron. Axons of VRi2-expressing neurons converge onto numerous glomeruli in the accessory olfactory bulb. The pattern of axonal projections is complex and variable. This wiring diagram is substantially different from that of the main olfactory system. The projection pattern is disrupted by deleting the coding region of VRi2, but an unrelated seven-transmembrane protein, the odorant receptor M71, can partially substitute for VRi2.
Published: 1999
Full Text: View/download PDF

46. Oxygen Toxicity in Mouse Lung: Pathways to Cell Death

Author: Pierre-François Piguet, Constance Barazzone, Stuart Horowitz, Ivan Rodriguez, and Yves Donati
Subjects: Lung Diseases, Necrosis, Amino Acid Chloromethyl Ketones/pharmacology, Bcl-X Protein, Clinical Biochemistry, Gene Expression, Apoptosis, ddc:616.07, Cathepsin D, Tumor Suppressor Protein p53/analysis/genetics, Amino Acid Chloromethyl Ketones, Caspase 1/metabolism, Mice, ddc:590, Diffuse alveolar damage, bcl-2-Associated X Protein, Mice, Knockout, Hyperoxia, ddc:618, Membrane Glycoproteins, biology, Caspase 3, Caspases/metabolism, RNA, Messenger/analysis, Caspase 1, Oxygen/toxicity, Apoptosis/drug effects/immunology, respiratory system, Caspase Inhibitors, Gene Expression/immunology, Proto-Oncogene Proteins c-bcl-2/genetics, Cell biology, Membrane Glycoproteins/analysis/genetics, Proto-Oncogene Proteins c-bcl-2, Pulmonary Alveoli/chemistry/enzymology/pathology, Caspases, medicine.symptom, Pulmonary and Respiratory Medicine, Antigens, CD95/analysis/genetics, Programmed cell death, Fas Ligand Protein, Blotting, Western, bcl-X Protein, Bcl-2-Associated X Protein, Cysteine Proteinase Inhibitors/pharmacology, Cysteine Proteinase Inhibitors, Bcl-2-associated X protein, Lung Diseases/chemically induced/metabolism/physiopathology, Proto-Oncogene Proteins, In Situ Nick-End Labeling, medicine, Animals, RNA, Messenger, fas Receptor, Fragmentation (cell biology), Molecular Biology, Cell Biology, Cathepsin D/metabolism, Mice, Inbred C57BL, Oxygen, Pulmonary Alveoli, Proto-Oncogene Proteins/genetics, Hyperoxia/metabolism/physiopathology, biology.protein, Tumor Suppressor Protein p53
Abstract: Mice exposed to 100% O2 die after 3 or 4 d with diffuse alveolar damage and alveolar edema. Extensive cell death is evident by electron microscopy in the alveolar septa, affecting both endothelial and epithelial cells. The damaged cells show features of both apoptosis (condensation and margination of chromatin) and necrosis (disruption of the plasma membrane). The electrophoretic pattern of lung DNA indicates both internucleosomal fragmentation, characteristic of apoptosis, and overall degradation, characteristic of necrosis. Hyperoxia induces a marked increase in RNA or protein levels of p53, bax, bcl-x, and Fas, which are known to be expressed in certain types of apoptosis. However, we did not detect an increased activity of proteases belonging to the apoptosis "executioner" machinery, such as CPP32 (caspase 3), ICE (caspase 1), or cathepsin D. Furthermore, administration of an ICE-like protease inhibitor did not significantly enhance the resistance to oxygen. Additionally, neither p53-deficient mice nor lpr mice (Fas null) manifested an increased resistance to hyperoxia-induced lung damage. These results show that both necrosis and apoptosis contribute to cell death during hyperoxia. Multiple apoptotic pathways seem to be involved in this, and an antiapoptotic strategy does not attenuate alveolar damage.
Published: 1998

47. Mouse Vaginal Opening Is an Apoptosis-Dependent Process Which Can Be Prevented by the Overexpression of Bcl2

Author: Ivan Rodriguez, Karim Khatib, Kimi Araki, Pierre Vassalli, and Jean-Claude Martinou
Subjects: Male, Genetically modified mouse, Pathology, medicine.medical_specialty, Transgene, Apoptosis, Mice, Transgenic, Distension, Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, Keratin, medicine, Animals, Humans, RNA, Messenger, Promoter Regions, Genetic, Molecular Biology, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Mucous Membrane, TUNEL assay, Estradiol, Epidermis (botany), Ovary, Genitalia, Female, Cell Biology, Keratin-10, Phenotype, Phosphoglycerate Kinase, Proto-Oncogene Proteins c-bcl-2, chemistry, Organ Specificity, Phosphopyruvate Hydratase, Vagina, Immunology, Keratins, Female, 030217 neurology & neurosurgery, Developmental Biology
Abstract: In the mouse, opening of the vaginal cavity to the skin is a late event, occurring around the fifth week of life; it can be induced in sexually immature mice by β-estradiol injections. We have generated two lines of transgenic mice expressing the human Bcl2 protein in a variety of tissues. The vaginal cavity of the transgenic females remained permanently closed, a condition completely resistant to β-estradiol injections; this was accompanied by a considerable distension of the genital tract. Histologic studies of vaginal sections at the time of opening to the skin in normal mice showed, by the TUNEL method which detects nuclei with fragmented DNA characteristic of apoptosis, that this event coincides with extensive apoptosis in the lower part of the vaginal mucosa, a process prevented in the bcl2 transgenic mice, which express Bcl2 in suprabasal epithelial cells and in subepithelial cells of the vaginal mucosa. In contrast, two lines of mice bearing a Bcl2 transgene placed under the control of a K10 keratin promoter, whose expression is restricted to the suprabasal layers of the epidermis, had a normal phenotype. Eyelids’ formation and opening of the external ear canals, which also occur after birth in the mouse, were not altered in any of these transgenic lines; histological study of eye and ear sections at the time of these events failed to detect apoptosis. In conclusion, the tissue remodeling required to complete maturation of the mouse female genital tract at the time of puberty is an hormonally triggered apoptosis-dependent process.
Published: 1997

48. Bcl-2 prevents activation of CPP32 cysteine protease and cleavage of poly (ADP-ribose) polymerase and U1-70 kD proteins in staurosporine-mediated apoptosis

Author: Jean-Claude Martinou, Rémy Sadoul, Ivan Rodriguez, and Sandrine Estoppey
Subjects: Proteases, Programmed cell death, biology, medicine.diagnostic_test, Chemistry, Poly ADP ribose polymerase, Proteolysis, Cell Biology, biology.organism_classification, Molecular biology, Cysteine protease, HeLa, Apoptosis, medicine, Staurosporine, Molecular Biology, medicine.drug
Abstract: Members of the the Bcl-2 and ICE/ced-3 gene families have been implicated as essential components in the control of the cell death pathway. Bcl-2 overexpression can prevent programmed cell death (PCD) in different cell types. ICE/ced-3-like proteases are synthesized as pro-enzymes and are activated by limited proteolysis. When overexpressed in diverse cell types, they trigger PCD. Bcl-2 can inhibit PCD mediated by these proteases, although as yet it is not clear at what specific step in the cell death pathway the protein acts. Here, we demonstrate that CPP32/Yama/Apopain, a member of the ICE/Ced-3 gene family, is processed during staurosporine-induced apoptosis in HeLa cells and that concomitant with CPP32 activation, two other proteins, poly (ADP-ribose) polymerase (PARP) and the U1-70 K small ribonucleoprotein, also undergo proteolysis. Overexpression of Bcl-2 prevents cleavage of CPP32, PARP and U1-70 K and protects HeLa cells from PCD. These results demonstrate that Bcl-2 controls PCD, by acting upstream of CPP32/Yama/Apopain.
Published: 1997

49. Targeting of the CD80/86 proinflammatory axis as a therapeutic strategy to prevent severe COVID-19

Author: Antonio Julià, Irene Bonafonte-Pardàs, Antonio Gómez, María López-Lasanta, Mireia López-Corbeto, Sergio H. Martínez-Mateu, Jordi Lladós, Iván Rodríguez-Nunez, Richard M. Myers, and Sara Marsal
Subjects: Medicine, Science
Abstract: Abstract An excessive immune response known as cytokine storm is the hallmark of severe COVID-19. The cause of this cytokine rampage is yet not known. Based on recent epidemiological evidence, we hypothesized that CD80/86 signaling is essential for this hyperinflammation, and that blocking this proinflammatory axis could be an effective therapeutic approach to protect against severe COVID-19. Here we provide exploratory evidence that abatacept, a drug that blocks CD80/86 co-stimulation, produces changes at the systemic level that are highly antagonistic of the proinflammatory processes elicited by COVID-19. Using RNA-seq from blood samples from a longitudinal cohort of n = 38 rheumatic patients treated with abatacept, we determined the immunological processes that are significantly regulated by this treatment. We then analyzed available blood RNA-seq from two COVID19 patient cohorts, a very early cohort from the epicenter of the pandemic in China (n = 3 COVID-19 cases and n = 3 controls), and a recent and larger cohort from the USA (n = 49 severe and n = 51 mild COVD-19 patients). We found a highly significant antagonism between SARS-CoV-2 infection and COVID-19 severity with the systemic response to abatacept. Analysis of previous single-cell RNA-seq data from bronchoalveolar lavage fluid from mild and severe COVID-19 patients and controls, reinforce the implication of the CD80/86 proinflammatory axis. Our functional results further support abatacept as a candidate therapeutic approach to prevent severe COVID-19.
Published: 2021
Full Text: View/download PDF

50. Overexpression of BCL-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia

Author: Claudio Pietra, Paola Albertini, Dominique Talabot, Michel Dubois-Dauphin, Ivan Rodriguez, Marc Missotten, Harald Frankowski, Julie Staple, Stefan Catsicas, and Jean-Claude Martinou
Subjects: Genetically modified mouse, Nervous system, Programmed cell death, Transgene, Enolase, Gene Expression, Mice, Transgenic, Biology, Mice, Ganglia, Spinal, Proto-Oncogene Proteins, medicine, Animals, Humans, Tissue Distribution, Promoter Regions, Genetic, Ganglion cell layer, Motor Neurons, Neurons, Retina, Cell Death, General Neuroscience, Brain, Optic Nerve, Cell biology, Facial Nerve, Phosphoglycerate Kinase, medicine.anatomical_structure, Proto-Oncogene Proteins c-bcl-2, Spinal Cord, nervous system, Ischemic Attack, Transient, Phosphopyruvate Hydratase, Immunology, Neuron
Abstract: Naturally occurring cell death (NOCD) is a prominent feature of the developing nervous system. During this process, neurons express bcl-2, a major regulator of cell death whose expression may determine whether a neuron dies or survives. To gain insight into the possible role of bcl-2 during NOCD in vivo, we generated lines of transgenic mice in which neurons overexpress the human BCL-2 protein under the control of the neuron-specific enolase (NSE) or phosphoglycerate kinase (PGK) promoters. BCL-2 overexpression reduced neuronal loss during the NOCD period, which led to hypertrophy of the nervous system. For instance, the facial nucleus and the ganglion cell layer of the retina had, respectively, 40% and 50% more neurons than normal. Consistent with this finding, more axons than normal were found in the facial and optic nerves. We also tested whether neurons overexpressing BCL-2 were more resistant to permanent ischemia induced by middle cerebral artery occlusion; in transgenic mice, the volume of the brain infarction was reduced by 50% as compared with wild-type mice. These animals represent an invaluable tool for studying the effects of increased neuronal numbers on brain function as well as the mechanisms that control the survival of neurons during development and adulthood.
Published: 1994

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Publication Type

Journal

Database

Publisher

85 results on '"Ivan Rodriguez"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources