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Your search keyword '"Irhamna Putri"' showing total 3 results
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3 results on '"Irhamna Putri"'

1. Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method

Author

Diana Savitri, Irhamna Putri, Warih Pulung Nugrahani, Medania Purwaningrum, and Aris Haryanto

Subjects
chd1 gene, conservation, sex identification, z and w chromosomes, Medicine, Biology (General), QH301-705.5
Abstract

Sex identification of endangered and protected birds in captivity is very important for conservation programs. Half of the world’s bird species are monomorphic, where male and female are difficult to distinguished morphologically, including cockatoos. Sex identification using molecular bird sexing is more accurate and applicable because it directly targets the sex chromosomes. The purpose of this study was to determine the sex of Sulphur‐crested cockatoo (Cacatua galerita) by detecting differences in the intron size of the chromodomain helicase DNA‐binding 1 (CHD1) gene on the Z and W chromosomes by polymerase chain reaction (PCR) method and to compare of plucked feathers and blood samples as DNA sources for molecular bird sexing. DNA was extracted from feather and blood samples from four C. galerita. Extracted DNA was amplified on the CHD1 gene by PCR method with P2, MP, and NP primers, which were visualized using agarose gel 1.5% under UV transilluminator with a wavelength of 280 nm. The resulting PCR product was detected at 392 bp for the CHD1 Z gene segment and 297 bp for CHD1 W gene segments, where males showed a single DNA band (ZZ) and females showed a double DNA band (ZW). Four C. galerita were 100% successfully determined, consisting of one female and three males. Electrophoresis results showed DNA bands from blood samples were thicker and brighter than DNA bands from feather samples.

Published
2021
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2. The Method of Total Erythrocyte and Leukocyte Counting in Raptors at Wildlife Rescue Centre (WRC) Jogja

Author

Warih Pulung Nugrahani Nugrahani, Andreas Bandang Hardian, Dorothea Vera Megarani, and Irhamna Putri Rahmawati Rahmawati

Subjects
Sample quality, education.field_of_study, Dilution ratio, business.industry, Shock (circulatory), Anesthesia, Cell density, Population, Medicine, medicine.symptom, business, Ulnar vein, education
Abstract

Most of raptor species rehabilitated for conservation purpose at WRC Jogja are eagles. Annually, we perform general raptor examination involving conventional routine hematologic examination. However, low sample quality and over dense blood cell population during total erythrocyte and leukocyte counting were deemed as obstacles. This study aimed to give wider insight in how total erythrocyte and leukocyte counting was performed at WRC Jogja to overcome those obstacles. Firstly, handling and restraint were done physically adapting the Aba method. One-to-two half millilitres of blood was collected from the ulnar vein using 3-ml-syringe with 24-gauge-needle which was then preserved in an EDTA-containing tube and proceeded to subsequent method no more than 3 hours after collection. No hypovolemic shock was observed. During total erythrocyte and leukocyte counting using Neubauer-ruled counting chamber, we modified the dilution factor to be 250-fold and 50-fold respectively. This method yielded a countable cell density and can be applied in other species in similar condition.

Published
2020

3. A Retrospective Study Of Morbidity In Captive Columbiformes Housed In Bristol Zoo Gardens And Wild Place Project, United Kingdom From 2000 – 2017

Author

Irhamna Putri Rahmawati

Subjects
Pediatrics, medicine.medical_specialty, Trichomoniasis, business.industry, Columbiformes, Medical record, Retrospective cohort study, General Medicine, Disease, medicine.disease, Infectious disease (medical specialty), Parasitic disease, Etiology, Medicine, business
Abstract

Medical records from 860 pigeons and doves of 24 species housed in Bristol Zoo Gardens and Wild Place Project, United Kingdom, from 2000 to 2017 were reviewed to determine the causes of morbidity in captive columbiformes. Accounted 205 cases (51.25%), infectious disease was found as the primary cause of morbidity, followed by 133 cases of trauma (33.25%), 51 (8.9%) cases of non-infectious disease, and 8 (2%) cases of husbandry-related problem. The main cause of infectious disease was parasite infection with 35% over 31% of bacterial, 26% of undetermined etiology and 9% of fungal infection. More specific, nematodiasis was the most frequent parasitic disease found, presented in 49 (69%) cases and followed by 10 (14%) trichomoniasis of all parasite infection. Meanwhile, chlamydiosis was a leading cause of bacterial infection with 22 (35%) cases recorded of over 63 bacterial infections. These results highlight the importance of preventative medicine implementation in captivity which aims to detect the disease earlier hence reducing the worsening physical condition and minimising the risk of both zoonotic and non-zoonotic disease transmissions. In addition, these findings contribute to the limited information available regarding the captive columbiformes morbidity

Published
2018

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