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The breast cancer has become a threat to women's lives at an increasing rate. The inadequate treatments of surgical procedures and chemotherapy affect negatively quality of patient life. Therefore, there has been an increase in demand for alternative and supportive agents, especially for the use of herbal products. The purpose of this study is to evaluate the in vitro anti-tumor activity of Lysimachia savranii in breast cancer cell lines compared to normal adipose tissue derived mesenchymal stem cells. The adipose tissue derived mesenchymal stem cells as normal cells with MCF-7 and, MDA-MB-231 breast cancer cells were cultured. IC50 doses of Lysimachia savranii extract for each cells were determined via MTT assay. The immunocytochemical staining was performed for the oxidative stress by eNOS, whereas apoptosis were analyzed by TUNEL method. The data exhibited with H-score and apoptotic index. The plant extract inhibited the proliferation and survival in the breast cancer cells compared to the stem cells. Lysimachia savranii extract caused the increase of eNOS staining for oxidative stress and the TUNEL labeling for apoptosis. The further molecular techniques should be used to determine the biologic activity of Lysimachia savranii extract and its content should be investigated.

The treatment of breast cancer includes chemotherapy, radiotherapy and chemotherapy. Recently, alternative agents have been searched for breast cancer, especially medicinal plants because their anti-cancer effects. It has been reported that the genus of Lysimachia has anti-proliferative effect on cancer cells. In our study we aimed to evaluate in vitro anti-cancer activity of Lysimachia savranii on migration of breast cancer cells. Breast cancer cell lines MCF-7 and MDA-MB-231 and also adipose tissue derived mesenchymal stem cells as normal cells. IC50 doses of Lysimachia savranii extract and Doxorubicin for each cells were calculated via MTT assay. In vitro wound model with (+) plus shape was created using pipette tips for migration analysis and IC50 doses were exposed. The oxidative stress, vascularization and apoptosis were analyzed by immunocytochemical staining of eNOS, iNOS, VEGF and TUNEL. Data was evaluated by Graphpad software using one-way analysis of variance. Lysimachia savranii and Doxorubicin have anti-proliferative effect on breast cancer cells. Breast cancer cell migration was inhibited by Lysimachia savranii. There was an increase of eNOS and iNOS stainings, and apoptotic cells whereas there was a decrease of VEGF staining. Our findings indicate that Lysimachia savranii extract is a potential herbal product for breast cancer therapy.

The number of breast cancer cases have increased gradually in women. During treatment of the breast cancer, chemotherapy needs adjuvant agents. Medicinal plants have an anticancerogenic effect in such cases. Our aim is to search the cytotoxic effect of Origanum minutiflorum O. Schwarz & P.H. Davis on breast cancer cells in vitro conditions. For this purpose MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived mesenchymal stem cells were used. Oregano oil from Origanum minutiflorum was exposed to the all cells and IC50 dose was calculated by MTT assay. The immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each cells. The results were evaluated using one-way-ANOVA by Graphpad software. The proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil did not show any cytotoxic effect in mesenchymal stem cells. It was determined that cytotoxic effect of oregano oil on cancer cells was occurred through increase of eNOS and p53 staining for survival and the TUNEL labeling for apoptosis, and decreased of PCNA staining for proliferation. According to our data, oregano oil has cytotoxic effect on breast cancer cell lines, and it needs to be analysis to detect active components for other biological activities.

Neuroblastoma cells have been used for antitumoral mechanisms such as stopping the cell cycle and inducing apoptosis. The genus of Lysimachia is studied to determine its anti-proliferative effect. The aim of this study is to show the cytotoxic effect of Lysimachia savranii on neuroblastoma cells in vitro conditions. Neuroblastoma cells and mesenchymal stem cells were cultured and treated with the extract of Lysimachia savranii and IC50 dose was evaluated. The effects on cell viability, oxidative stress, neurite inhibition and apoptosis of Lysimachia savranii at dose of IC50 were studied. In neuroblastoma cells, the rates of eNOS and iNOS stainings used for analysis of oxidative stress were greater than mesenchymal stem cells, and the difference was statistically significant (p < 0.005). TUNEL assay to determine cell apoptosis in neuroblastoma cells was greater than mesenchymal stem cells and also the difference was statistically significant (p < 0.005). The effects of Lysimachia savranii was showed out in vitro on neuroblastoma cells and this plants can be analysis for its cytotoxic effect in vivo models.

The natural products are promising products for anti-proliferative effects in cancer. In this study our aim was to analyze the neurotoxic effect of Origanum minutiflorum O. Schwarz & PH. Davis on neuroblastoma cells in in vitro conditions. The neuroblastoma cells were treated with Oregano oil from Origanum minutiflorum and doxorubicin, and cytotoxic activities of these agents were evaluated by MTT assay for IC50 doses. The immunocytochemistry staining of eNOS, iNOS and TUNEL assay were performed. The results were analyzed using Graphpad software for one-way-ANOVA. The immunoreactivities of eNOS and iNOS were increased in neuroblastoma cells treated with oregano oil and doxorubicin. Both of Oregano oil and doxorubicin showed inhibitory activity against neuroblastoma cells via oxidative stress. Also the number of apoptotic cells was greater than the control group. The oregano oil enhanced the cytotoxic activity of doxorubicin. The compound of oregano oil should be considered with the other neuroblastoma cells for neurotoxicity.

WOS: 000365685800014, PubMed ID: 26457595, Further elucidation of the molecular mechanisms underlying lung cancer (LC) is essential for the development of new effective therapeutic agents. Recently, involvement of Wnt antagonists in oncogenesis has been demonstrated in several cancers. The investigation of their contribution to lung carcinogenesis is still under investigation. We aimed to investigate whether there is a susceptibility or preventive effect of Wnt antagonist gene polymorphisms on the development and/or prognosis of LC. We investigated 110 LC patients and 160 controls. Single-nucleotide polymorphisms of Wnt antagonist genes including DKK2 (rs17037102), DKK3 (rs3206824), DKK3 intron4 G/C (rs7396187), DKK4 (rs2073664), and sFRP4 (rs1802074) were analyzed using nested polymerase chain reaction and restriction fragment length polymorphism. Results showed that patients with DKK3 AA compared with controls have a decreased risk of LC (adjusted for smoking habit, body mass index, and familial history) (P = 0.02; odds ratio [OR],0.08; 95% confidence interval [95% CI], 0.01-0.7). It was found that, for sFRP4 polymorphism, patients with GG and GA genotypes versus AA genotype controls showed a decreased risk for LC (P = 0.01; [OR, 0.19; 95% CI, 0.05-0.73 for GG genotype]; [OR = 0.18, 95% CI, 0.04-0.72 for GA genotype]). In addition, a decreased risk of LC was also found for the genotype combination of DKK3 (rs3206824) GG and sFRP4 AG + GG (P = 0.004; OR, 0.12; 95% CI, 0.02-0.58). We suggest that these 2 polymorphisms have a protective effect on LC in this study., Cumhuriyet University Research Committee [T-504], Supported by Cumhuriyet University Research Committee Grant T-504.