1. Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage
- Author
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Emerson B. Lee, Hyun-Jin Kim, Young-Jin Kim, Hayyoung Lee, Hyung-Min Jeon, Jie-Oh Lee, Young Sil Kim, Doo-Jin Kim, Sang M. Park, Chang-Ung Kim, and Geunyoung Cho
- Subjects
Salmonella typhimurium ,medicine.medical_treatment ,T cell ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Bioengineering ,Hemagglutinin Glycoproteins, Influenza Virus ,02 engineering and technology ,Microbiology ,Cell Line ,03 medical and health sciences ,Mice ,Immune system ,Adjuvants, Immunologic ,medicine ,Animals ,Humans ,General Materials Science ,Secretion ,030304 developmental biology ,0303 health sciences ,Immunity, Cellular ,Mice, Inbred BALB C ,biology ,Chemistry ,Hemagglutinin ,021001 nanoscience & nanotechnology ,Immunity, Humoral ,Nanostructures ,Ferritin ,medicine.anatomical_structure ,Influenza A virus ,Ferritins ,biology.protein ,bacteria ,Molecular Medicine ,Female ,Antibody ,0210 nano-technology ,Adjuvant ,Flagellin - Abstract
Hemagglutinin (HA) displayed on a ferritin nano-cage has been shown to be effective in generating a potent immune response against a broad range of influenza infections. Here, we showed that conjugation of flagellin together with HA to the exterior surface of the ferritin cage greatly enhanced not only the humoral immune response in mice but also antigen-specific T cell responses that include Th1 cytokine secretion. The effect of flagellin remained essentially unchanged when the molar ratio of flagellin to HA was reduced from 1:1 to 1:3. Injection of the ferritin-HA-flagellin cage provided protection against lethal virus challenge in mice. We used a small immunoglobulin fragment VL12.3 as a convenient method for attaching HA and flagellin to the ferritin cage. This attachment method can be used for rapid screening of a variety of protein cages and nano-assemblies to identify the most suitable carrier and adjuvant proteins for the target antigen. more...
- Published
- 2017