40 results on '"Eun Kyung Jang"'
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2. Sub-Classification of Lateral Cervical Lymph Node Metastasis in Papillary Thyroid Carcinoma by Pathologic Criteria.
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Min Ji Jeon, Won Gu Kim, Eun Kyung Jang, Yun Mi Choi, Dong Eun Song, Tae-Yon Sung, Jong Ho Yoon, Ki-Wook Chung, Suck Joon Hong, Jin-Sook Ryu, Ji Min Han, Tae Yong Kim, Young Kee Shong, and Won Bae Kim
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Medicine ,Science - Abstract
BACKGROUND:Lateral cervical lymph node (LCLN) metastasis, or pathologic N1b disease, is an important risk factor in papillary thyroid carcinoma (PTC). However, many patients have favorable prognosis even with pathologic N1b patients in clinical practice. The study aims to identify high- and intermediate-risk groups based on initial pathologic characteristics in these patients. PATIENTS:This study included 518 classical PTC patients confirmed as pathologic N1b at initial surgery between 2001 and 2010. All patients underwent a single fixed activity (5.6 GBq) of radioactive I-131 remnant ablation. RESULTS:Patients with a primary tumor larger than 4 cm, gross extrathyroidal extension, metastatic LN larger than 3 cm, or greater than 10 metastatic LCLN were classified as high-risk group. These comprehensive pathologic criteria were retrieved from cox proportional hazard models. Twenty two percent of patients (n = 113) were classified as high-risk and 78% (n = 405) as intermediate-risk group. Successful ablation was identified in only 32% of the patients in the high-risk group and 61% in the intermediate-risk group (p < 0.001). The difference between the two risk groups was independent to gender. There was a significant difference in disease-free survival between the high- and intermediate- risk N1b groups during 5.1 years of median follow-up (84% vs. 59%, p < 0.001). Distant metastasis was more prevalent in the high-risk group (20%) than in the intermediate-risk group (4%, p < 0.001). CONCLUSIONS:The prognosis of PTC patients with LCLN metastasis varies depending on initial pathologic characteristics. We proposed the comprehensive pathologic criteria for sub-classification of N1b into high- and intermediate-risk groups and this sub-classification may permit personalized management of N1b PTC patients.
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- 2015
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3. Changes in the pulmonary function test after radioactive iodine treatment in patients with pulmonary metastases of differentiated thyroid cancer.
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Eun Kyung Jang, Won Gu Kim, Ho-Cheol Kim, Jin-Won Huh, Hyemi Kwon, Yun Mi Choi, Min Ji Jeon, Tae Yong Kim, Young Kee Shong, Jin-Sook Ryu, and Won Bae Kim
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Medicine ,Science - Abstract
Pulmonary function test (PFT) is a useful tool for an objective assessment of respiratory function. Impaired pulmonary function is critical for the survival and quality of life in patients with pulmonary metastases of solid cancers including thyroid cancer. This study aimed to evaluate clinical factors associated with severely impaired pulmonary function by serial assessment with PFT in patients with pulmonary metastasis of differentiated thyroid cancer (DTC) who received radioactive iodine treatment (RAIT).This retrospective study enrolled 31 patients who underwent serial PFTs before and after RAIT for pulmonary metastasis of DTC. We evaluated the risk factors for severe impairment of pulmonary function.The median age of the patients was 44.1 years and 18 of them were female patients. Severe impairment of pulmonary function was observed in five patients (16%) after a median of three RAITs (cumulative I-131 activity = 20.4 GBq). These patients were older and more frequently had mild impairment of baseline pulmonary function, respiratory symptoms, or progressive disease compared with patients with stable pulmonary function. Neither cumulative dose nor number of RAIT was associated with decreased pulmonary function. Coexisting pulmonary diseases, presence of respiratory symptoms, and metastatic disease progression were significantly associated with severe decrease in forced vital capacity during follow-up (p =.047, p =.011, and p =.021, respectively).Pulmonary function was severely impaired during follow-up in some patients with pulmonary metastasis of DTC after a high-dose RAITs. Neither the number of RAIT nor the cumulative I-131 activity was associated with decreased pulmonary function. Serial PFT might be considered for some high-risk patients during follow-up.
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- 2015
- Full Text
- View/download PDF
4. 2020 Korean Guidelines for Cardiopulmonary Resuscitation. Part 8. Neonatal resuscitation
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Hye Won Park, Ju Sun Heo, Su Yeong Kim, Yong Sung Choi, Han Suk Kim, Sung Oh Hwang, Ai Rhan Ellen Kim, Eun Kyung Jang, Ah Young Oh, Geum Joon Cho, and Chan Wook Park
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medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Emergency Medicine ,medicine ,MEDLINE ,Guideline ,Cardiopulmonary resuscitation ,Emergency Nursing ,Intensive care medicine ,business ,Neonatal resuscitation - Published
- 2021
5. Cloning and expression of the insecticidal toxin gene 'tccB' from Photorhabdus temperata M1021 in Escherichia coli expression system
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Gun-Seok Park, Sung-Jun Hong, Ihsan Ullah, Byung Kwon Jung, Won-Chan Kim, Jae-Ho Shin, Khalid M. Al-Ghamdi, Eun-Kyung Jang, Muhammad Faisal Siddiqui, Yasir Anwar, Yeong-Jun Park, Abdur Rahim Khan, and Bassam Oudh Al-Johny
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0106 biological sciences ,0301 basic medicine ,animal structures ,Expression vector ,biology ,Toxin ,fungi ,biology.organism_classification ,medicine.disease_cause ,01 natural sciences ,Microbiology ,010602 entomology ,03 medical and health sciences ,030104 developmental biology ,Gene cassette ,Insect Science ,Bacillus thuringiensis ,Cosmid ,medicine ,Gene ,Escherichia coli ,Photorhabdus - Abstract
Photorhabdus spp. has a high molecular weight Tc toxin with insecticidal activity. These toxins have been suggested as an alternative to BT toxin from Bacillus thuringiensis. Herein, we constructed a cosmid library with the genome of M1021 and screened the Escherichia coli clones showing insect toxicity by injecting each clone into Galleria mellonella larvae. In a total of 1020 clones, one clone with high insecticidal activity was selected and the nucleotide sequence of the cosmid of the clone was determined. In cosmid PtC28, a gene with 87% homology to the tccB gene of Photorhabdus temperata was found. Consequently, we have isolated the tccB gene cassette from the M1021 and expressed in E. coli expression vectors. The toxin was produced in the form of inclusion bodies but the denatured and refolded recombinant TccB showed strong mortality to the G. mellonella larvae.
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- 2020
6. Comparison of the Pain-relieving Effects of Human Milk, Sucrose, and Distilled Water during Examinations for Retinopathy of Prematurity: A Randomized Controlled Trial
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Sung Mi Lee, Hyun Jin Seo, Eun Kyung Jang, Eun Joo Huh, Keum Sik Jo, and Hyejung Lee
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Sucrose ,business.industry ,Human milk ,lcsh:R ,Pain ,lcsh:Medicine ,Retinopathy of prematurity ,medicine.disease ,Pediatrics ,law.invention ,chemistry.chemical_compound ,Distilled water ,chemistry ,Randomized controlled trial ,law ,Anesthesia ,Pediatrics, Perinatology and Child Health ,Medicine ,Original Article ,Analgesia ,business - Abstract
Purpose This study compared the pain-relieving effects of human milk, sucrose, and distilled water during examinations for retinopathy of prematurity. Methods Forty-five preterm infants were randomly assigned to receive a pacifier dipped in one of three solutions: human milk (n=14), 24% sucrose (n=15), or distilled water (n=16), 2 minutes before an eye examination. Their pain score, pulse rate, and oxygen saturation were measured at three time points: 5 minutes before the examination, 30 seconds after speculum introduction, and 2 minutes after the examination. Results The infants’ mean gestational age and weight at birth were 33.1±2.1 weeks and 1,842±470 g, respectively. There were no between-group differences in pain relief during the eye examination. The pain score significantly increased both during (p
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- 2019
7. Association between thyroid autoimmunity and Helicobacter pylori infection
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Won Bae Kim, Min Ji Jeon, Yun Mi Choi, Eui Young Kim, Won Gu Kim, Eun Kyung Jang, Young Kee Shong, and Tae Yong Kim
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Adult ,Male ,medicine.medical_specialty ,Autoimmunity ,030209 endocrinology & metabolism ,Autoantigens ,Iodide Peroxidase ,Gastroenterology ,Immunoglobulin G ,Helicobacter Infections ,Autoimmune thyroiditis ,03 medical and health sciences ,0302 clinical medicine ,Thyroid-stimulating hormone ,Iron-Binding Proteins ,Internal medicine ,Republic of Korea ,Prevalence ,medicine ,Humans ,Endocrinology-Metabolism ,Aged ,Autoantibodies ,Thyroid ,Helicobacter pylori ,biology ,business.industry ,Thyroiditis, Autoimmune ,Odds ratio ,Middle Aged ,biology.organism_classification ,medicine.disease ,medicine.anatomical_structure ,biology.protein ,Original Article ,Female ,030211 gastroenterology & hepatology ,Antibody ,business ,Body mass index - Abstract
Background/Aims There have been controversial reports linking Helicobacter pylori infection to autoimmune thyroid disease (AITD). However, data regarding the relationship are limited for Asian populations, which have an extremely high prevalence of H. pylori infection. We performed this study to investigate the association between H. pylori infection and AITD in Koreans. Methods This study involved adults aged 30 to 70 years who had visited a health promotion center. A total of 5,502 subjects were analysed. Thyroid status was assessed by free thyroxine, thyroid stimulating hormone, and anti-thyroid peroxidase antibody (TPO-Ab). Immunoglobulin G (IgG) antibodies to H. pylori were measured as an indication of H. pylori infection. We compared the prevalence of TPO-Ab in subjects with and without H. pylori infection. Results H. pylori IgG antibodies were found in 2,875 subjects (52.3%), and TPO-Ab were found in 430 (7.8%). Individuals positive for H. pylori Ab were older than those negative for H. pylori Ab (p < 0.01). The proportion of females was significantly higher in the TPO-Ab positive group (41.0% vs. 64.2%, p < 0.01). Prevalence of TPO-Ab positivity was higher in subjects with H. pylori infection (8.6% vs. 7.00%, p = 0.03), and this association was significant after adjusting for age, sex, and body mass index (odds ratio, 1.02; 95% confidence interval, 1.00 to 1.03; p = 0.04). Conclusions In our study, prevalence of TPO-Ab positivity is more frequent in subjects with H. pylori infection. Our findings suggest H. pylori infection may play a role in the development of autoimmune thyroiditis.
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- 2017
8. Numerical modeling of sedimentation control scenarios in the approach channel of the Nakdong River Estuary Barrage, South Korea
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Un Ji, Gwon-Han Kim, and Eun-Kyung Jang
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Hydrology ,geography ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,Stratigraphy ,0208 environmental biotechnology ,Sediment ,Geology ,Estuary ,02 engineering and technology ,Sedimentation ,Sediment control ,01 natural sciences ,Deposition (geology) ,020801 environmental engineering ,Dredging ,medicine ,Flushing ,medicine.symptom ,Channel (geography) ,0105 earth and related environmental sciences - Abstract
The effects of sedimentation reduction at the Nakdong River Estuary Barrage (NREB) in Korea were quantitatively analyzed with respect to different sediment control methods using the calibrated and validated two-dimensional model. The countermeasures of sediment dredging, sediment flushing, channel geometry change, and a combination of flushing and channel geometry change were examined for the approach channel of the NREB. The flood event and channel geometries of the 3.8 km section upstream of the NREB surveyed before and after dredging in 2007 were used for modeling conditions. As a result, the half of sediments dredged in 2007 could be eliminated naturally by floods without dredging. The numerical simulation of sediment flushing indicated that the deposition height decreased in the entire simulation section with the minimum and maximum reductions from 0.3 m to 1.3 m in deposition height. The channel contraction method produced quantitatively the largest amount of sedimentation reduction and sediment flushing and dredging followed. Sedimentation reduction by a combination of flushing and channel contraction was up 10% compared to the individual method of channel contraction.
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- 2016
9. Usefulness of Measuring Thyroid Stimulating Antibody at the Time of Antithyroid Drug Withdrawal for Predicting Relapse of Graves Disease
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Jin-Sook Ryu, Won Gu Kim, Hyemi Kwon, Suyeon Park, Tae Yong Kim, Won Bae Kim, Min Ji Jeon, Eun Kyung Jang, Young Kee Shong, and Mijin Kim
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Drug ,medicine.medical_specialty ,endocrine system ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Graves' disease ,media_common.quotation_subject ,Thyrotropin receptor Antibody ,Immunoglobulins ,030209 endocrinology & metabolism ,Trab ,Gastroenterology ,Hyperthyroidism ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,03 medical and health sciences ,Drug withdrawal ,0302 clinical medicine ,Endocrinology ,Internal medicine ,medicine ,media_common ,lcsh:RC648-665 ,biology ,business.industry ,Thyroid ,medicine.disease ,Prognosis ,eye diseases ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Immunology ,Clinical Study ,biology.protein ,Graves disease ,Original Article ,Antibody ,Thyroid stimulating antibody ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Background Hyperthyroidism relapse in Graves disease after antithyroid drug (ATD) withdrawal is common; however, measuring the thyrotropin receptor antibody (TRAb) at ATD withdrawal in order to predict outcomes is controversial. This study compared measurement of thyroid stimulatory antibody (TSAb) and thyrotropin-binding inhibitory immunoglobulin (TBII) at ATD withdrawal to predict relapse. Methods This retrospective study enrolled patients with Graves disease who were treated with ATDs and whose serum thyroid-stimulating hormone levels were normal after receiving low-dose ATDs. ATD therapy was stopped irrespective of TRAb positivity after an additional 6 months of receiving the minimum dose of ATD therapy. Patients were followed using thyroid function tests and TSAb (TSAb group; n=35) or TBII (TBII group; n=39) every 3 to 6 months for 2 years after ATD withdrawal. Results Twenty-eight patients (38%) relapsed for a median follow-up of 21 months, and there were no differences in baseline clinical characteristics between groups. In the TSAb group, relapse was more common in patients with positive TSAb at ATD withdrawal (67%) than patients with negative TSAb (17%; P=0.007). Relapse-free survival was shorter in TSAb-positive patients. In the TBII group, there were no differences in the relapse rate and relapse-free survivals according to TBII positivity. For predicting Graves disease relapse, the sensitivity and specificity of TSAb were 63% and 83%, respectively, whereas those of TBII were 28% and 65%. Conclusion TSAb at ATD withdrawal can predict the relapse of Graves hyperthyroidism, but TBII cannot. Measuring TSAb at ATD withdrawal can assist with clinical decisions making for patients with Graves disease.
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- 2016
10. Diminished Quality of Life and Increased Brain Functional Connectivity in Patients with Hypothyroidism After Total Thyroidectomy
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Won Bae Kim, Brian F. O'Donnell, Ho Sung Kim, Dae-Jin Kim, Jin Pyo Hong, Yun Mi Choi, Hang Joon Jo, Tae Yong Kim, Eun Kyung Jang, Young Kee Shong, and Yong-Wook Shin
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Adult ,Male ,Oncology ,medicine.medical_specialty ,Health Status ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Thyroid function tests ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Hypothyroidism ,Surveys and Questionnaires ,Internal medicine ,medicine ,Humans ,Thyroid Neoplasms ,Thyroid cancer ,medicine.diagnostic_test ,Resting state fMRI ,Depression ,business.industry ,Thyroid ,Original StudiesThyroid Dysfunction: Hypothyroidism, Thyrotoxicosis, and Thyroid Function Tests ,Brain ,Magnetic resonance imaging ,Human brain ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Mood ,medicine.anatomical_structure ,Quality of Life ,Thyroidectomy ,Female ,Nerve Net ,business ,030217 neurology & neurosurgery ,Hormone - Abstract
Acute hypothyroidism induced by thyroid hormone withdrawal (THW) in patients with thyroid cancer after total thyroidectomy can affect mood and quality of life (QoL). While loss or dysregulation of thyroid hormone (TH) has these well-known behavioral consequences, the effects of TH alterations on brain function are not well understood. Resting state functional connectivity (FC) measured by functional magnetic resonance imaging (fMRI) allows non-invasive evaluation of human brain function. This study therefore examined whether THW affects resting state FC and whether changes in FC correlate with the mood or QoL of the patients with THW status.Twenty-one patients who had undergone total thyroidectomy for thyroid cancer were recruited. Resting state fMRI scanning of the brain, thyroid function tests, and administration of the 12-Item Short Form Health Survey (SF-12) and the Patient Health Questionnaire-9 (PHQ-9) were performed before and after two weeks of THW. Regional homogeneity (ReHo), one of the measures of resting state FC, was calculated, and each voxel was compared between before and after THW in 19 patients. The ReHo values were extracted from the regions of interest showing within-group differences in ReHo values after THW, and correlations of ReHo values with thyrotropin (TSH) levels, total score of the PHQ-9, and composite scores of the SF-12 were statistically evaluated.Higher ReHo was observed after THW in the brain cortical regions across primary motor and sensory, visual, and association cortices. Among the regions, the ReHo values in the bilateral pre- and postcentral gyri, bilateral middle occipito-temporal cortices, the left precuneus, and the left lingual gyrus showed positive correlations with serum TSH levels after THW. Higher ReHo values in the bilateral pre- and postcentral gyri, the left middle temporo-occipital cortices, and the left ligual gyrus correlated with the lower mental component summary score from the SF-12, while higher ReHo values in the bilateral pre- and postcentral gyri correlated with higher total scores in the PHQ-9.Local brain FC is increased in the acute hypothyroid state. Higher FC correlates with a poorer mental QoL and increased depression in the hypothyroid state.
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- 2016
11. Usefulness of NRAS codon 61 mutation analysis and core needle biopsy for the diagnosis of thyroid nodules previously diagnosed as atypia of undetermined significance
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Hyemi Kwon, Eun Kyung Jang, Won Bae Kim, Young Kee Shong, Jene Choi, Jeong Hyun Lee, Won Gu Kim, Tae Yong Kim, Dong Eun Song, Yun Mi Choi, Min Ji Jeon, Jung Hwan Baek, and Eui Young Kim
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Adult ,Male ,Thyroid nodules ,Pathology ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,DNA Mutational Analysis ,Thyroid Gland ,030209 endocrinology & metabolism ,medicine.disease_cause ,Malignancy ,GTP Phosphohydrolases ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Biopsy ,medicine ,Atypia ,Humans ,Thyroid Nodule ,Thyroid cancer ,Thyroid neoplasm ,Aged ,medicine.diagnostic_test ,business.industry ,Membrane Proteins ,Nodule (medicine) ,Middle Aged ,medicine.disease ,Fine-needle aspiration ,030220 oncology & carcinogenesis ,Female ,Biopsy, Large-Core Needle ,Radiology ,medicine.symptom ,business - Abstract
A repeat fine needle aspiration (FNA) is recommended for thyroid nodules diagnosed as atypia of undetermined significance (AUS) in a previous cytology. We evaluated the utility of NRAS codon 61 (NRAS61) mutation analysis and core needle biopsy (CNB) for the diagnosis of thyroid nodules previously diagnosed as AUS. This study enrolled 236 patients who underwent both NRAS61 mutation analysis and CNB of thyroid nodules previously diagnosed as AUS at cytology. The NRAS61 mutation was detected in 36 nodules and was more frequently detected in the AUS and follicular neoplasm (FN)/suspicious for follicular neoplasm (SFN) categories, as determined by histological analysis of CNB, than in the benign group (p = 0.005). Sixty-one patients underwent surgery, and 29 nodules were finally diagnosed as malignant after surgery. Among 61 patients who underwent surgery, nodules with the NRAS61 mutation (42-65 %) had a significantly higher malignancy rate than nodules with wild-type NRAS61 (7-37 %, p = 0.038). The association between malignancy and the NRAS61 mutation was significant after adjusting for age, sex, nodule size, and histological diagnosis of CNB (p = 0.01). NRAS61 mutation analysis together with CNB could be helpful for arriving at a clinical decision in patients with thyroid nodules showing AUS in a previous cytology.
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- 2015
12. Negative Expression of CPSF2 Predicts a Poorer Clinical Outcome in Patients with Papillary Thyroid Carcinoma
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Won Gu Kim, Dong Eun Song, Hyemi Kwon, Tae Yon Sung, Yun Mi Choi, Tae Yong Kim, Suyeon Park, Mijin Kim, Suck Joon Hong, Eun Kyung Jang, Young Kee Shong, Won Bae Kim, Min Ji Jeon, and Yangsoon Park
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Adult ,Male ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,DNA Mutational Analysis ,Kaplan-Meier Estimate ,Biology ,Disease-Free Survival ,Thyroid carcinoma ,Endocrinology ,Cancer stem cell ,Republic of Korea ,Biomarkers, Tumor ,Odds Ratio ,Prevalence ,medicine ,Carcinoma ,Humans ,Neoplasm Invasiveness ,Thyroid Neoplasms ,Neoplasm Metastasis ,Thyroid cancer ,Proportional Hazards Models ,Regulation of gene expression ,Gene Expression Profiling ,Cleavage And Polyadenylation Specificity Factor ,Thyroidectomy ,Middle Aged ,Prognosis ,medicine.disease ,Carcinoma, Papillary ,Gene Expression Regulation, Neoplastic ,Gene expression profiling ,Treatment Outcome ,Thyroid Cancer, Papillary ,Tissue Array Analysis ,Multivariate Analysis ,Mutation ,Neoplastic Stem Cells ,Cancer research ,Immunohistochemistry ,Female ,Neoplasm Recurrence, Local - Abstract
The BRAF(V600E) mutation is a promising prognostic biomarker for patients with papillary thyroid carcinoma (PTC), but its prevalence differs widely among different geographic regions. A recent study reported that loss of the Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) gene was associated with increased cellular invasion, cancer stem cells, and aggressiveness of PTC. This study aimed at evaluating CPSF2 protein expression as a prognostic marker for PTC in a region with a high prevalence of the BRAF(V600E) mutation, Korea.This study included 159 patients with classical PTC who underwent a total thyroidectomy and received ablative doses of (131)I. The expression of CPSF2 protein was evaluated by immunohistochemistry and graded semi-quantitatively. The presence of the BRAF(V600E) mutation was evaluated by direct sequencing.Negative protein expression of CPSF2 was observed in 34 (21.3%) of the 159 PTCs. In multivariate analysis, negative CPSF2 expression was significantly associated with cervical lymph node metastasis (odds ratio [OR]=2.56, p=0.28), and distant metastasis (OR=3.48, p=0.02). After adjusting for age, sex, tumor size, extrathyroidal invasion, lymphovascular invasion, and the BRAF(V600E) mutation, the CPSF2-negative group had a significantly lower recurrence-free survival compared to the CPSF2-positive group (hazard ratio=2.14, p=0.03).Negative protein expression of CPSF2 is independently associated with a poor clinical outcome in PTC. CPSF2 could be a useful prognostic marker for PTC in regions with a high prevalence of the BRAF(V600E) mutation.
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- 2015
13. Lack of Associations between Body Mass Index and Clinical Outcomes in Patients with Papillary Thyroid Carcinoma
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Min Ji Jeon, Dong Eun Song, Suck Joon Hong, Yun Mi Choi, Hyemi Kwon, Won Bae Kim, Tae Yong Kim, Won Gu Kim, Mijin Kim, Jung Hwan Baek, Eun Kyung Jang, and Young Kee Shong
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Oncology ,medicine.medical_specialty ,Multivariate analysis ,lcsh:RC648-665 ,business.industry ,Endocrinology, Diabetes and Metabolism ,Thyroid neoplasms ,Retrospective cohort study ,medicine.disease ,Prognosis ,Obesity ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Surgery ,Thyroid carcinoma ,Endocrinology ,Quartile ,Internal medicine ,medicine ,Clinical Study ,Original Article ,Stage (cooking) ,business ,Pathological ,Body mass index - Abstract
Background: Obesity is associated with aggressive pathological features and poor clinical outcomes in breast and prostate cancers. In papillary thyroid carcinoma (PTC), these relationships remain still controversial. This study aimed to evaluate the associations between body mass index (BMI) and the clinical outcomes of patients with PTC. Methods: This retrospective study included 1,189 patients who underwent total thyroidectomy for PTCs equal to or larger than 1 cm in size. Clinical outcomes were evaluated and compared based on the BMI quartiles. Results: There were no significant associations between BMI quartiles and primary tumor size, extrathyroidal invasion, cervical lymph node metastasis, or distant metastasis. However, an increase in mean age was associated with an increased BMI (P for trend
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- 2015
14. Thyroglobulin Level in Fine-Needle Aspirates for Preoperative Diagnosis of Cervical Lymph Node Metastasis in Patients with Papillary Thyroid Carcinoma: Two Different Cutoff Values According to Serum Thyroglobulin Level
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Min Ji Jeon, Yu-Mi Lee, Eun Kyung Jang, Tae Yong Kim, Ki-Wook Chung, Young Kee Shong, Yun Mi Choi, Suck Joon Hong, Jong Ho Yoon, Jeong Hyun Lee, Jung Hwan Baek, Won Gu Kim, Tae-Yon Sung, and Won Bae Kim
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Adult ,Male ,medicine.medical_specialty ,Pathology ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Thyroglobulin ,Gastroenterology ,Metastasis ,Thyroid carcinoma ,Endocrinology ,Predictive Value of Tests ,Internal medicine ,Biomarkers, Tumor ,Carcinoma ,medicine ,Humans ,Cutoff ,Thyroid Neoplasms ,skin and connective tissue diseases ,Endoscopic Ultrasound-Guided Fine Needle Aspiration ,Thyroid cancer ,Lymph node ,business.industry ,Reproducibility of Results ,Middle Aged ,medicine.disease ,Carcinoma, Papillary ,body regions ,surgical procedures, operative ,medicine.anatomical_structure ,ROC Curve ,Thyroid Cancer, Papillary ,Area Under Curve ,Lymphatic Metastasis ,Predictive value of tests ,Lymph Node Excision ,Female ,Lymph Nodes ,business - Abstract
Measurement of thyroglobulin (Tg) in the washout fluid of fine-needle aspirates (FNA-Tg) is useful for diagnosis of lymph node (LN) metastasis in papillary thyroid carcinoma (PTC). However, the cutoff value of FNA-Tg in the preoperative state is not defined clearly. This study aimed to evaluate the optimal cutoff value of preoperative FNA-Tg according to serum Tg level.FNA-Tg was measured in 135 PTC patients (160 LNs) for preoperative diagnosis of cervical LN metastasis.Of the 160 LNs, 119 (74%) were surgically removed and 110 (69%) were diagnosed as malignant. When we adopted a FNA-Tg of 1.0 μg/L as the cutoff value, the sensitivity and specificity were 99% and 76%, respectively. FNA-Tg levels were correlated with serum Tg levels (Pearson's coefficient 0.42, p=0.002) and the FNA-Tg levels of 12 of the 50 benign LNs were above 1.0 μg/L. We classified the LNs into two groups according to serum Tg level regardless of anti-Tg antibody status: a low Tg group (≤1.0 μg/L, n=22, 14%) and a high Tg group (1.0 μg/L, n=138, 86%). In the low Tg group, the sensitivity and specificity of the FNA-Tg cutoff value of 1.0 μg/L were 93% and 100%, respectively. In the high Tg group, the sensitivity and specificity of the FNA-Tg cutoff value of 19.0 μg/L were 93% and 100%, respectively. A Tg ratio (FNA-Tg level divided by serum Tg level) of 0.5 gave an improved diagnostic performance (sensitivity, 98%; specificity, 98%) in the high Tg group.FNA-Tg levels in the preoperative state are affected by serum Tg levels when they exceeded 1.0 μg/L. For the preoperative diagnosis of metastatic cervical LNs, it seems reasonable to employ different cutoff values of FNA-Tg depending on serum Tg levels. We propose the use of an optimal cutoff value of FNA-Tg of 1.0 μg/L in patients with low serum Tg levels and a Tg ratio of 0.5 in those with high serum Tg levels irrespective of thyroglobulin antibody status.
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- 2015
15. Metformin Is Associated with a Favorable Outcome in Diabetic Patients with Cervical Lymph Node Metastasis of Differentiated Thyroid Cancer
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Won Bae Kim, Hyemi Kwon, Min Ji Jeon, Eun Kyung Jang, Young Kee Shong, Eui Young Kim, Won Gu Kim, Tae Yong Kim, and Yun Mi Choi
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Oncology ,medicine.medical_specialty ,Pathology ,Clinical Thyroidology / Original Paper ,endocrine system diseases ,business.industry ,Endocrinology, Diabetes and Metabolism ,Cancer ,Type 2 diabetes ,Lymph node metastasis ,medicine.disease ,Metformin ,Text mining ,Internal medicine ,Diabetes mellitus ,medicine ,Favorable outcome ,business ,Thyroid cancer ,medicine.drug - Abstract
Type 2 diabetes is known to increase the risk and progression of certain types of cancer. Metformin treatment of diabetic patients is reported to have beneficial effects on some cancers. We evaluated the clinical outcome of diabetic patients with differentiated thyroid cancer (DTC) according to metformin treatment.We reviewed 943 patients diagnosed with DTC after total thyroidectomy between 1995 and 2005 in a tertiary hospital. The study involved 60 diabetic patients and 210 control patients matched for age, sex, body mass index (BMI), and tumor size.There were no differences in the clinicopathological features and disease-free survival (DFS) between diabetic patients and the control group over 8.9 years of follow-up. Of the diabetic patients with DTC, 35 patients (58%) were treated with metformin. There were no differences in age, sex, BMI, tumor size, antidiabetic medication, glycated hemoglobin, or C-peptide levels in metformin and nonmetformin groups. However, cervical lymph node (LN) metastasis was more prevalent in the metformin group than in the nonmetformin group (OR 3.52, p = 0.035). Among diabetic patients with cervical LN metastasis of DTC, the metformin subgroup (17.1 years) was associated with longer DFS than the nonmetformin subgroup (8.6 years) (HR 0.16, p = 0.021); metformin treatment was also associated with longer DFS in this subgroup in multivariate analysis after adjusting age, BMI, duration of diabetes, presence of tumor at resection margin, and serum thyroglobulin level at ablation (HR 0.03, p = 0.035).Metformin treatment is associated with low recurrence in diabetic patients with cervical LN metastasis of DTC.
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- 2015
16. Association between neck ultrasonographic findings and clinico‐pathological features in the follicular variant of papillary thyroid carcinoma
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Tae Yong Kim, Won Bae Kim, Dong Eun Song, Won Gu Kim, Yun Mi Choi, Min Ji Jeon, Hyemi Kwon, Jeong Hyun Lee, Eun Kyung Jang, Young Kee Shong, and Jung Hwan Baek
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Adult ,Male ,Thyroid nodules ,Pathology ,medicine.medical_specialty ,Adenoma ,Endocrinology, Diabetes and Metabolism ,Thyroid Gland ,Carcinoma, Papillary, Follicular ,Malignancy ,Metastasis ,Thyroid carcinoma ,Endocrinology ,Cytology ,Internal medicine ,Humans ,Medicine ,Lymph node ,Retrospective Studies ,Suspicious for Malignancy ,business.industry ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,Female ,business ,Neck - Abstract
OBJECTIVE The follicular variant of papillary thyroid carcinoma (FVPTC) has multiple histological subtypes. Clinical outcomes of FVPTC are variable depending on the subtypes. This study evaluated the association of pre-operative ultrasonographic (US) findings and clinico-pathological features of FVPTC. PATIENTS This retrospective study enrolled patients with FVPTC (n = 70), size-matched classical variant of papillary thyroid carcinoma (CPTC, n = 328), follicular carcinoma (n = 85) and follicular adenoma (FA, n = 120). We defined the histological subtypes of FVPTC as infiltrative (I-FVPTC; n = 19) or encapsulated (E-FVPTC; n = 51) according to the presence of a fibrous capsule. Pre-operative US was reviewed using a US scoring system and classified into low US score (n = 42) and high US score (n = 28). RESULTS The median US score for FVPTC was lower than CPTC (2 vs 7, P < 0·001), but higher than FA (2 vs 0, P < 0·001). The median US score for I-FVPTC was significantly higher than E-FVPTC (4 vs 2, P = 0·009). I-FVPTC was more likely to be diagnosed as a malignancy or suspicious for malignancy on cytology than E-FVPTC (P = 0·002). The cumulative risks of cervical lymph node (LN) or distant metastasis according to tumour size were significantly higher in I-FVPTC than E-FVPTC (all P < 0·001). The cumulative risks for cervical LN metastasis or capsular invasion according to tumour size were significantly higher in FVPTC with high US score than FVPTC with low US score (P = 0·005, P < 0·001, respectively). CONCLUSIONS Pre-operative US findings of thyroid nodules were associated with not only histological subtypes, but also the clinical behaviour in FVPTC.
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- 2014
17. Standardized Thyroid Cancer Mortality in Korea between 1985 and 2010
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Won Bae Kim, Eun Kyung Jang, Min Ji Jeon, Young Kee Shong, Tae Yong Kim, Won Gu Kim, Yun Mi Choi, and Hyemi Kwon
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Endocrinology, Diabetes and Metabolism ,Population ,MEDLINE ,Thyroid neoplasms ,Bioinformatics ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,World health ,Endocrinology ,Medicine ,Mortality ,education ,Thyroid cancer ,Standard Population ,education.field_of_study ,lcsh:RC648-665 ,business.industry ,Mortality rate ,Cancer survival ,medicine.disease ,Mortality data ,Clinical Study ,Original Article ,Erratum ,business ,Demography ,Standard population - Abstract
Background The prevalence of thyroid cancer has increased very rapidly in Korea. However, there is no published report focusing on thyroid cancer mortality in Korea. In this study, we aimed to evaluate standardized thyroid cancer mortality using data from Statistics Korea (the Statistical Office of Korea). Methods Population and mortality data from 1985 to 2010 were obtained from Statistics Korea. Age-standardized rates of thyroid cancer mortality were calculated according to the standard population of Korea, as well as World Health Organization (WHO) standard population and International Cancer Survival Standard (ICSS) population weights. Results The crude thyroid cancer mortality rate increased from 0.1 to 0.7 per 100,000 between 1985 and 2010. The pattern was the same for both sexes. The age-standardized mortality rate (ASMR) for thyroid cancer for Korean resident registration population increased from 0.19 to 0.67 between 1985 and 2000. However, it decreased slightly, from 0.67 to 0.55, between 2000 and 2010. When mortality was adjusted using the WHO standard population and ICSS population weights, the ASMR similarly increased until 2000, and then decreased between 2000 and 2010. Conclusion Thyroid cancer mortality increased until 2000 in Korea. It started to decrease from 2000.
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- 2014
18. A cut-off value of basal serum calcitonin for detecting macroscopic medullary thyroid carcinoma
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Dong Eun Song, Won Bae Kim, Suck Joon Hong, Jung Hwan Baek, Tae Yong Kim, Won Gu Kim, Min Ji Jeon, Hyemi Kwon, Jin-Sook Ryu, Yun Mi Choi, Eun Kyung Jang, and Young Kee Shong
- Subjects
Calcitonin ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Thyroid Gland ,Multiple Endocrine Neoplasia Type 2a ,Medical Oncology ,Sensitivity and Specificity ,Thyroiditis ,Autoimmune thyroiditis ,Thyroid carcinoma ,Basal (phylogenetics) ,Endocrinology ,Predictive Value of Tests ,Internal medicine ,Cytology ,Biomarkers, Tumor ,medicine ,Carcinoma ,Humans ,Thyroid Neoplasms ,Renal Insufficiency, Chronic ,Retrospective Studies ,Ultrasonography ,business.industry ,Thyroid ,Thyroiditis, Autoimmune ,Reproducibility of Results ,Proton Pump Inhibitors ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,Carcinoma, Medullary ,Predictive value of tests ,Female ,business ,Follow-Up Studies - Abstract
SummaryObjective Serum calcitonin (CT) level is used to detect medullary thyroid carcinoma (MTC), but the cut-off level is unclear. We aimed at identifying the optimal cut-off value of basal serum CT levels for detecting MTC. Design and patients We retrospectively enrolled patients with hypercalcitoninemia (≥2·9 pmol/l) who had undergone thyroid ultrasonography (US) and subsequent work-up between 2001 and 2013 at Asan Medical Center. We divided patients into four groups: proven MTC (group 1, n = 93), pathologically proven non-MTC after surgery (group 2, n = 57), benign single nodule by cytology (group 3, n = 68) and patients without nodules on US (group 4, n = 24). Measurement Basal serum CT levels were evaluated. Results The median CT level of group 1 (119·5 pmol/l) was significantly higher than those of other groups (4·0, 3·8 and 3·8 pmol/l, P
- Published
- 2014
19. Identification and Characterization of the Insecticidal Toxin 'Makes Caterpillars Floppy' in Photorhabdus temperata M1021 Using a Cosmid Library
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Gun-Seok Park, Yeong-Jun Park, Ihsan Ullah, Jae-Ho Shin, Jin-Ho Shin, Eun-Kyung Jang, Min-Sung Kim, Abdur Rahim Khan, Sung-Jun Hong, Yunyoung Kwak, Byung Kwon Jung, and JungBae Choi
- Subjects
Insecticides ,insecticidal toxin ,Galleria mellonella ,makes caterpillar floppy ,Photorhabdus temperata ,Tenebrio molitor ,Virulence Factors ,Health, Toxicology and Mutagenesis ,Bacterial Toxins ,Molecular Sequence Data ,Virulence ,lcsh:Medicine ,Sequence alignment ,Moths ,Biology ,Toxicology ,medicine.disease_cause ,Article ,Microbiology ,Bacterial Proteins ,Escherichia coli ,medicine ,Consensus sequence ,Animals ,Amino Acid Sequence ,Tenebrio ,Peptide sequence ,Phylogeny ,fungi ,lcsh:R ,Cosmids ,biology.organism_classification ,Pathogenicity island ,Larva ,Cosmid ,Photorhabdus - Abstract
Photorhabdus temperata is an entomopathogenic enterobacterium; it is a nematode symbiont that possesses pathogenicity islands involved in insect virulence. Herein, we constructed a P. temperata M1021 cosmid library in Escherichia coli XL1-Blue MRF` and obtained 7.14 x 10(5) clones. However, only 1020 physiologically active clones were screened for insect virulence factors by injection of each E. coli cosmid clone into Galleria mellonella and Tenebrio molitor larvae. A single cosmid clone, PtC1015, was consequently selected due to its characteristic virulent properties, e. g., loss of body turgor followed by death of larvae when the clone was injected into the hemocoel. The sequence alignment against the available sequences in Swiss-Prot and NCBI databases, confirmed the presence of the mcf gene homolog in the genome of P. temperata M1021 showing 85% homology and 98% query coverage with the P. luminescens counterpart. Furthermore, a 2932 amino acid long Mcf protein revealed limited similarity with three protein domains. The N-terminus of the Mcf encompassed consensus sequence for a BH3 domain, the central region revealed similarity to toxin B, and the C-terminus of Mcf revealed similarity to the bacterial export domain of ApxIVA, an RTX-like toxin. In short, the Mcf toxin is likely to play a role in the elimination of insect pests, making it a promising model for use in the agricultural field.
- Published
- 2014
20. Low Levels of Serum Vitamin D3 Are Associated with Autoimmune Thyroid Disease in Pre-Menopausal Women
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Eun Kyung Jang, Young Kee Shong, Min Ji Jeon, Won Bae Kim, Ji Min Han, Tae Yong Kim, Jung Hwan Baek, Won Gu Kim, Hong-Kyu Kim, Seung Hun Lee, Sung Jin Bae, and Yun Mi Choi
- Subjects
Male ,Vitamin ,endocrine system ,medicine.medical_specialty ,endocrine system diseases ,Cross-sectional study ,Endocrinology, Diabetes and Metabolism ,Autoantigens ,Iodide Peroxidase ,Gastroenterology ,Thyroiditis ,vitamin D deficiency ,chemistry.chemical_compound ,Endocrinology ,Iron-Binding Proteins ,Internal medicine ,Humans ,Medicine ,Aged ,Autoantibodies ,Calcifediol ,Ultrasonography ,biology ,business.industry ,Thyroid ,Original StudiesThyroid Dysfunction: Hypothyroidism, Thyrotoxicosis, and Thyroid Function Tests ,Thyroiditis, Autoimmune ,Autoantibody ,Middle Aged ,Vitamin D Deficiency ,medicine.disease ,Cross-Sectional Studies ,medicine.anatomical_structure ,Premenopause ,chemistry ,biology.protein ,Female ,Antibody ,business - Abstract
Low serum vitamin D levels have been associated with several autoimmune diseases, but their association with thyroid autoimmunity is unclear. We evaluated the association of serum vitamin D levels with the prevalence of autoimmune thyroid disease (AITD).Our cross-sectional study included subjects who underwent routine health checkups, which included assays of serum 25-hydroxy vitamin D3 [25(OH)D3] and anti-thyroid peroxidase antibody (TPO-Ab), as well as thyroid ultrasonography (US) between 2008 and 2012 at the Asan Medical Center. We defined AITD according to the levels of TPO-Ab and US findings.A total of 6685 subjects (58% male; 42% female) were enrolled for this study. Overall prevalence of TPO-Ab positivity and both TPO-Ab/US positivity were 10.1% (6.3% male; 15.3% female) and 5.4% (2.3% male; 9.7% female) respectively. In female subjects, mean serum 25(OH)D3 levels were significantly lower in the TPO-Ab(+) (22.0 vs. 23.5 ng/mL, p=0.030) and TPO-Ab(+)/US(+) groups (21.6 vs. 23.4 ng/mL, p=0.027) compared with the control group, respectively. According to the levels of serum 25(OH)D3, the prevalence of TPO-Ab positivity (21.2%, 15.5%, and 12.6% in deficient, insufficient, and sufficient group, respectively; p=0.001) and both TPO-Ab and US positivity (14.7%, 9.9%, and 7.1% in deficient, insufficient, and sufficient group, respectively; p0.001) decreased in female subjects. Interestingly, this pattern was significant only in pre-menopausal women (p=0.003 and p0.001; respectively), but not in postmenopausal women. Multivariate analysis indicated that the adjusted odds ratios (OR) for AITD among those in the 25(OH)D3-deficient [TPO-Ab(+): OR 1.95, p=0.001; TPO-Ab(+)/US(+): OR 2.36, p0.001] and -insufficient groups [TPO-Ab(+): OR 1.31, p=0.043; TPO-Ab(+)/US(+): OR 1.50, p=0.017] were significantly increased when compared with the sufficient group.The levels of serum vitamin D were significantly lower in pre-menopausal women with AITD. Vitamin D deficiency and insufficiency were significantly associated with AITD in pre-menopausal women.
- Published
- 2014
21. Long-Term Survival of a Patient with Pulmonary Artery Intimal Sarcoma after Sequential Metastasectomies of the Thyroid and Adrenal Glands
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Ji Min Han, Tae Yong Kim, Seong Hee Ahn, Eun Kyung Jang, Young Kee Shong, Duck Jong Han, Gyungyup Gong, Seong Chul Kim, Won Bae Kim, Yun Mi Choi, and Min Ji Jeon
- Subjects
medicine.medical_specialty ,lcsh:RC648-665 ,business.industry ,Endocrinology, Diabetes and Metabolism ,Adrenalectomy ,medicine.medical_treatment ,Thyroid ,Metastasectomy ,Case Report ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Surgery ,Endocrinology ,medicine.anatomical_structure ,medicine.artery ,Pulmonary Valve Replacement ,Endocrine organ ,Pulmonary artery ,medicine ,Endocrine system ,Histopathology ,Radiology ,Pulmonary artery intimal sarcoma ,business ,Endarterectomy - Abstract
Cancer metastases to the thyroid or adrenal gland are uncommon. Furthermore, cases showing long-term survival after surgical resection of those metastatic tumors are rare. We report a case of pulmonary artery intimal sarcoma with metastases to the thyroid and adrenal glands sequentially that was successfully treated with sequential metastasectomies. A 62-year-old woman presented with a 4-week history of dyspnea on exertion and facial edema in November 1999. Echocardiography and chest computed tomography (CT) revealed an embolism-like mass in the pulmonary trunk. Pulmonary artery endarterectomy with pulmonary valve replacement was performed, and histopathology revealed pulmonary artery intimal sarcoma. A thyroid nodule was found by chest CT in November 2001 (2 years after initial surgery). During follow-up, this lesion showed no change, but we decided to obtain fine needle aspiration cytology (FNAC) in August 2004 (4.7 years after initial surgery). FNAC revealed atypical spindle cells suggestive of metastatic intimal sarcoma. She underwent total thyroidectomy. During follow-up, a right adrenal gland mass was detected by chest CT in March 2006 (6.3 years after initial surgery), and adrenalectomy was done, which also revealed metastatic sarcoma. She has been followed up without any evidence of recurrent disease until May 2012 (12.5 years after initial surgery).
- Published
- 2013
22. Papillary thyroid carcinoma arising from a thyroglossal duct cyst: a single institution experience
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Won Bae Kim, Won Gu Kim, Yun Mi Choi, Ji Min Han, Dong Eun Song, Tae Yong Kim, Suck Joon Hong, Eun Kyung Jang, Young Kee Shong, and Min Ji Jeon
- Subjects
Adult ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Thyroglossal duct ,Thyroid Gland ,Iodine Radioisotopes ,Thyroid carcinoma ,Endocrinology ,Republic of Korea ,medicine ,Carcinoma ,Humans ,Thyroid Neoplasms ,Thyroid cancer ,Aged ,Academic Medical Centers ,business.industry ,Thyroid ,Thyroidectomy ,Thyroglossal cyst ,Neck dissection ,Middle Aged ,medicine.disease ,Carcinoma, Papillary ,Thyroglossal Cyst ,Surgery ,Radiography ,Treatment Outcome ,medicine.anatomical_structure ,Thyroid Cancer, Papillary ,Lymphatic Metastasis ,Neck Dissection ,Female ,Radiotherapy, Adjuvant ,Neoplasm Recurrence, Local ,Radiopharmaceuticals ,business - Abstract
Thyroid cancers arising from a thyroglossal duct cyst (TGDC) are rarely reported. No clear consensus exists regarding optimal management. In this light, TGDC carcinomas recently treated at Asan Medical Center, as well as previously reported cases in the literature, were reviewed. There were ten patients who were diagnosed with TGDC carcinoma at our institution. All patients underwent pre-operative fine-needle aspiration biopsy (FNAB). Nine patients were suspected of having papillary carcinoma following cytology. The Sistrunk operation (SO) was performed in four patients, SO with total thyroidectomy (SO/TT) was performed in three patients, and SO/TT with neck dissection was performed in three patients. Six patients who received total thyroidectomy underwent radioactive iodine (RAI) therapy and T4 suppression. With a median follow-up period of 28.5 months, two patients showed recurrence and one of them died of the disease. We analyzed 163 cases from 1990 to 2012 with three or more cases TGDC carcinoma, including the present study. Among 48 patients who underwent FNAB, 75% had papillary thyroid carcinoma (PTC). SO, SO/TT, or SO/TT with neck dissection was performed in 27%, 41%, and 32% of patients, respectively. Among 119 patients who received total thyroidectomy, 36% had concomitant PTC in the thyroid. Among 52 patients who received neck dissection, 69% had cervical nodal involvement. The results of our review suggest that when TGDC carcinoma is suspected, ultrasonography and, if necessary, FNAB should be performed. If these tests reveal a suspected lesion in the thyroid or lymph node, SO/TT and lymph node dissection should be performed.
- Published
- 2013
23. Genomic Alterations of Anaplastic Thyroid Carcinoma Detected by Targeted Massive Parallel Sequencing in a BRAF(V600E) Mutation-Prevalent Area
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Tae Yong Kim, Hyemi Kwon, Min Ji Jeon, Se Jin Jang, Sung-Min Chun, Won Bae Kim, Deokhoon Kim, Won Gu Kim, Eun Kyung Jang, Young Kee Shong, and Dong Eun Song
- Subjects
0301 basic medicine ,Male ,Proto-Oncogene Proteins B-raf ,endocrine system diseases ,Genotype ,Endocrinology, Diabetes and Metabolism ,DNA Mutational Analysis ,Biology ,medicine.disease_cause ,Thyroid Carcinoma, Anaplastic ,Thyroid carcinoma ,Proto-Oncogene Proteins p21(ras) ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Endocrinology ,medicine ,Humans ,Thyroid Neoplasms ,Thyroid cancer ,Exome sequencing ,Aged ,Sanger sequencing ,Aged, 80 and over ,Massive parallel sequencing ,Point mutation ,High-Throughput Nucleotide Sequencing ,Middle Aged ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Mutation ,Cancer research ,symbols ,Female ,KRAS ,V600E - Abstract
Anaplastic thyroid carcinoma (ATC), the most aggressive type of thyroid cancer, has no effective therapy. Due to its dismal prognosis, it is vital to understand the genetic alterations of ATC and identify effective molecular targets. Targeted next-generation sequencing was performed to investigate the mutational profile of ATC using a massive parallel sequencing approach.DNA from formalin-fixed, paraffin-embedded archival samples of 11 ATCs and normal matched pairs were used. A total of 48 genetic alterations were identified by targeted exome sequencing. These alterations were validated by mass spectrometric genotyping and direct Sanger sequencing.The most commonly mutated gene was BRAF, identified in 10 samples (91%), all showing the V600E point mutation. A KRAS point mutation was observed in the one sample (9%) without the BRAF(V600E) mutation. All 11 ATCs harbored BRAF or RAS mutations, reflecting the possibility that differentiated thyroid carcinomas progress to ATCs after the accumulation of mutations. A loss of function mutation of TP53 was observed in eight samples (73%), a PIK3CA mutation was observed in two samples (18%), and a frameshift mutation of PTEN was observed in one sample (9%). Twenty-eight novel mutated genes were found that had not previously been associated with ATC. Of these, loss of function mutations of NF2, KMT2D, and PKHD1 were repeatedly seen in three samples (27%), two samples (18%), and two samples (18%), respectively. Using direct Sanger sequencing, two samples (18%) were also found with a RASAL1 mutation. KMT2D and RASAL1 mutations were significantly associated with shorter ATC patient survival.This comprehensive analysis of ATCs using targeted massive parallel sequencing identified several novel mutations in ATCs, such as loss of function mutations of NF2 or KMT2D. Future studies are needed to confirm the role of these novel mutations as independent drivers of ATC development.
- Published
- 2016
24. Isolation and characterisation of the entomopathogenic bacterium, Photorhabdus temperata producing a heat stable insecticidal toxin
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Ihsan Ullah, Jae-Ho Shin, Kyeong-Yeoll Lee, Eun-Kyung Jang, and Min-Sung Kim
- Subjects
0106 biological sciences ,animal structures ,biology ,Toxin ,fungi ,Plant Science ,Horticulture ,Heterorhabditis ,biology.organism_classification ,Proteinase K ,medicine.disease_cause ,01 natural sciences ,Microbiology ,Galleria mellonella ,010602 entomology ,Hemolymph ,biology.protein ,medicine ,Agronomy and Crop Science ,Photorhabdus ,Bacteria ,010606 plant biology & botany ,Symbiotic bacteria - Abstract
Entomopathogenic nematodes symbiotically associated with Photorhabdus spp. invade the larvae of susceptible insects and Photorhabdus bacteria are released into the insect hemolymph, which produce toxins and kill the insect larvae. In this study, five symbiotic bacteria isolates from soil nematodes, Heterorhabditis spp., have been reported. The bacteria were screened for insecticidal toxicity against larvae of Galleria mellonella. All were identified as different strains of Photorhabdus temperata via 16 s rDNA sequencing. The insecticidal activity was highest after 3–4 days of pure culture and the level of toxicity was higher in culture supernatant than in the cell pellet. The heat stability of the insecticidal activity was tested by heat treatments for 30 min at the range of 25°C to 100°C. Out of all isolated strains, P. temperata strains J4 and J5 produced heat-stable toxins. The supernatants extracted from the culture of these strains preserved up to 95% of insecticidal activity after heat treatment for 30 min at 80°C. The insecticidal activities of culture supernatants of all the selected strains were mostly maintained after proteinase K treatment. Based upon these findings, it can be inferred that the insecticidal toxins produced by Photorhabdus spp. were not pure protein substances.
- Published
- 2011
25. Status and Subject of Art Therapy in the Local Social Community in the Seoul Area
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Eun-Kyung Jang
- Subjects
Value (ethics) ,Medical education ,Psychotherapist ,business.industry ,Process (engineering) ,media_common.quotation_subject ,Art therapy ,Social Welfare ,Variety (cybernetics) ,Medicine ,Consciousness ,Duration (project management) ,business ,Welfare ,media_common - Abstract
This thesis in the social welfare-related institutions in the Seoul area, art therapy and the therapist based on the status of welfare-related agencies in the community of art therapy is to present possibilities and challenges. Research results presented by Health and Welfare, where seven of the 14 kinds of social welfare facilities, participate in the survey and the target organ, 45.1% of art therapy`s role in social welfare-related institutions and gradually establish the location was the result. Art therapy is to conduct the current difficulties in institutions about the environmental factors related to a professional therapist and art therapist in the therapist`s expansion as a major research and maintenance was network. Welfare-related agencies and community relationships we maintain mutual relationship, and are formed within the community have an effect on consciousness and collectivization. To provide the opportunity which realize the value in the art therapy by applying more to expand the variety of professional method. First, secure the facility of art therapy environment and the therapists. Second, if the art therapy association secures the integrated system, it makes a connection with professional therapists to improve recruitment environment in the art therapy community and therapists need to be addressed by all involved is a challenge. Third, a therapist for the duration of training in order to access a variety of academic and clinical research is the importance of the process of learning the training and supervision is to discuss the issue.
- Published
- 2009
26. Characterization of dextran produced from Leuconostoc citreum S5 strain isolated from Korean fermented vegetable
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Sam-Pin Lee, In-Jung Kim, Eun-Kyung Jang, Ji-Hyun Seo, In-Seon Lee, Seung-Chun Park, Min-Jung Son, and Oh-Sik Kwon
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Sucrose ,food.ingredient ,biology ,food and beverages ,Fructose ,Titratable acid ,General Chemistry ,biology.organism_classification ,medicine.disease_cause ,Biochemistry ,Industrial and Manufacturing Engineering ,chemistry.chemical_compound ,Dextran ,food ,chemistry ,Leuconostoc citreum ,Skimmed milk ,medicine ,Leuconostoc ,Fermentation ,Food science ,Food Science ,Biotechnology - Abstract
The production of dextran was optimized by a novel Leuconostoc citreum, which was isolated from Korean traditional fermented vegetable, Dongchimi. This strain was identified as Leuconostoc citreum S5 by a physiological analysis, API-kit analysis, 16-rDNA sequencing and RAPD-PCR. The type and production of dextrans were greatly affected by the sucrose concentration, food ingredients and fermentation time. In particular, the addition of skim milk and potato powder greatly increased the consistency of the culture broth after fermentation for 12 h at 30 °C, resulting in the maximum values after 72 h. The culture broth with the highest consistency, was obtained from the defined medium containing 20% sucrose, 1.5% skim milk and 0.5% potato powder, and it showed typical pseudoplastic behavior with a pH of 4.07, 1.12% titratable acidity, 472.5 Pa of the elasticity modulus (G′) and 85.0 Pa of viscosity modulus (G″). In addition, total dextran is composed of soluble dextran (33.2 g/L) and insoluble dextran (29.8 g/L). The conversion yield of sucrose was decreased by an increase, in the sucrose concentration in a defined medium, remaining sucrose and some of glucose and fructose. High-molecular weight dextran (2,000 kDa), as a minor fraction, was produced by increasing the sucrose. With the addition of food ingredients such as skim milk and potato powder, however, the conversion of sucrose is complete, showing only some fructose as a by-product. Low-molecular weight dextran (1,100 kDa) was only produced regardless of sucrose concentration.
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- 2007
27. Sub-Classification of Lateral Cervical Lymph Node Metastasis in Papillary Thyroid Carcinoma by Pathologic Criteria
- Author
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Dong Eun Song, Won Bae Kim, Won Gu Kim, Ji Min Han, Min Ji Jeon, Ki-Wook Chung, Tae Yong Kim, Tae-Yon Sung, Yun Mi Choi, Jong Ho Yoon, Jin-Sook Ryu, Suck Joon Hong, Eun Kyung Jang, and Young Kee Shong
- Subjects
Adult ,Male ,medicine.medical_specialty ,Pathology ,lcsh:Medicine ,Disease ,Disease-Free Survival ,Metastasis ,Thyroid carcinoma ,Risk Factors ,medicine ,Carcinoma ,Humans ,Thyroid Neoplasms ,Risk factor ,lcsh:Science ,Neoplasm Staging ,Multidisciplinary ,medicine.diagnostic_test ,Clinical pathology ,Lateral cervical lymph node ,business.industry ,lcsh:R ,Middle Aged ,medicine.disease ,Carcinoma, Papillary ,Treatment Outcome ,Positron emission tomography ,Thyroid Cancer, Papillary ,Lymphatic Metastasis ,Multivariate Analysis ,lcsh:Q ,Female ,Neoplasm Recurrence, Local ,business ,Research Article - Abstract
BACKGROUND:Lateral cervical lymph node (LCLN) metastasis, or pathologic N1b disease, is an important risk factor in papillary thyroid carcinoma (PTC). However, many patients have favorable prognosis even with pathologic N1b patients in clinical practice. The study aims to identify high- and intermediate-risk groups based on initial pathologic characteristics in these patients. PATIENTS:This study included 518 classical PTC patients confirmed as pathologic N1b at initial surgery between 2001 and 2010. All patients underwent a single fixed activity (5.6 GBq) of radioactive I-131 remnant ablation. RESULTS:Patients with a primary tumor larger than 4 cm, gross extrathyroidal extension, metastatic LN larger than 3 cm, or greater than 10 metastatic LCLN were classified as high-risk group. These comprehensive pathologic criteria were retrieved from cox proportional hazard models. Twenty two percent of patients (n = 113) were classified as high-risk and 78% (n = 405) as intermediate-risk group. Successful ablation was identified in only 32% of the patients in the high-risk group and 61% in the intermediate-risk group (p < 0.001). The difference between the two risk groups was independent to gender. There was a significant difference in disease-free survival between the high- and intermediate- risk N1b groups during 5.1 years of median follow-up (84% vs. 59%, p < 0.001). Distant metastasis was more prevalent in the high-risk group (20%) than in the intermediate-risk group (4%, p < 0.001). CONCLUSIONS:The prognosis of PTC patients with LCLN metastasis varies depending on initial pathologic characteristics. We proposed the comprehensive pathologic criteria for sub-classification of N1b into high- and intermediate-risk groups and this sub-classification may permit personalized management of N1b PTC patients.
- Published
- 2015
28. Serum vitamin D3 levels are not associated with thyroid cancer prevalence in euthyroid subjects without autoimmune thyroid disease
- Author
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Eun Kyung Jang, Young Kee Shong, Min Ji Jeon, Sung Jin Bae, Won Bae Kim, Ji Min Han, Yun Mi Choi, Hong-Kyu Kim, Tae Yong Kim, and Won Gu Kim
- Subjects
Male ,Time Factors ,endocrine system diseases ,Gastroenterology ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,Prevalence ,Euthyroid ,Endocrinology-Metabolism ,Vitamin D ,Thyroid cancer ,Ultrasonography ,biology ,Thyroid ,Middle Aged ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Original Article ,Seasons ,Antibody ,Vitamin ,Adult ,medicine.medical_specialty ,endocrine system ,Thyroid Hormones ,Seoul ,Biopsy, Fine-Needle ,Thyroid neoplasms ,030209 endocrinology & metabolism ,Subgroup analysis ,03 medical and health sciences ,Thyroid-stimulating hormone ,Internal medicine ,Vitamin D and neurology ,medicine ,Humans ,Aged ,Autoantibodies ,Calcifediol ,Thyroid autoimmunity ,business.industry ,medicine.disease ,Vitamin D Deficiency ,Endocrinology ,Cross-Sectional Studies ,chemistry ,Case-Control Studies ,biology.protein ,business ,Biomarkers - Abstract
BACKGROUND/AIMS Previous studies have suggested that elevated serum vitamin D levels might protect against thyroid cancer. Elevated serum thyroid stimulating hormone levels and autoimmune thyroid disease (AITD) are suggested to be thyroid cancer promoting factors but have not been well controlled in previous studies. We designed the present study to evaluate whether serum vitamin D levels are associated with thyroid cancer in euthyroid patients with no clinical evidence of AITD. METHODS This cross-sectional study included subjects who underwent routine health check-ups, including serum 25-hydroxy vitamin D3 (25(OH)D3) levels, anti-thyroid peroxidase antibody (TPO-Ab), and thyroid ultrasonography (US). Inclusion criteria were euthyroid, negative TPO-Ab, and no evidence of AITD by US findings. Thyroid cancer diagnoses were based on fine needle aspiration cytology and/or postsurgical histopathological findings. RESULTS We enrolled 5,186 subjects (64% male, 37% female) in this study, including 53 patients (1%) with a diagnosis of thyroid cancer (33 males, 20 females). Mean 25(OH)D3 levels were similar between the thyroid cancer and control groups (p = 0.20). Subgroup analysis according to sex or seasonal variation also revealed no differences in 25(OH)D3 levels between the two groups. Based on the levels of 25(OH)D3, there was no significant difference in the prevalence of thyroid cancer; the prevalence was 0.71%, 0.94%, 1.40%, and 0.82% in the deficient, insufficient, sufficient, and excess groups, respectively (p = 0.64). CONCLUSIONS The levels of serum 25(OH)D3 are not associated with thyroid cancer prevalence in euthyroid subjects with no clinical evidence of AITD.
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- 2015
29. NRAS Codon 61 Mutation Is Associated with Distant Metastasis in Patients with Follicular Thyroid Carcinoma
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So Young Sim, Won Bae Kim, Ji Min Han, Dong Eun Song, Min Ji Jeon, Hyemi Kwon, Tae Yong Kim, Won Gu Kim, Eun Kyung Jang, Young Kee Shong, and Yun Mi Choi
- Subjects
Neuroblastoma RAS viral oncogene homolog ,Adenoma ,Adult ,Male ,Proto-Oncogene Proteins B-raf ,Pathology ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,DNA Mutational Analysis ,medicine.disease_cause ,Thyroid carcinoma ,Endocrinology ,Adenocarcinoma, Follicular ,medicine ,Humans ,Neoplasm Invasiveness ,HRAS ,Thyroid Neoplasms ,Neoplasm Metastasis ,Codon ,Aged ,Mutation ,Hyperplasia ,business.industry ,Thyroidectomy ,Age Factors ,Thyroid Cancer and Nodules ,Middle Aged ,medicine.disease ,Treatment Outcome ,Cancer research ,ras Proteins ,Adenocarcinoma ,Female ,KRAS ,business - Abstract
Background: Known factors related to distant metastases in follicular thyroid carcinoma (FTC) included age, primary tumor size, and invasiveness. Distant metastasis is a main cause of death in FTC patients. Several studies showed that the presence of RAS mutations is also associated with poor clinical outcomes. We analyzed RAS mutations in FTC with distant metastases, FTC without a distant metastasis, follicular adenoma (FA), and nodular hyperplasia (NH). Furthermore, we elucidated the relationship between RAS mutations and clinical outcomes in FTC patients. Methods: We selected patients who underwent a thyroidectomy for FTC with distant metastases (n=28), size matched FTC specimens without a distant metastasis (n=28), FA (n=17), and NH (n=12). NRAS, HRAS, and KRAS mutations were assessed using direct sequencing. Results: Among 85 patients, 39 patients (46%) had RAS mutations. The NRAS codon 61 mutation (n=21; 25%) was the most common point mutation. HRAS codon 61, KRAS codon 12/13, and KRAS codon 61 mutations were found in 7, 6, and 4 patients, respectively. A NRAS codon 12/13 mutation was found in only 1 patient, and a HRAS codon 12/13 mutation was not found. RAS mutations were significantly more common in the FTC than FA or NH groups. Especially, the NRAS codon 61 mutation was associated with distant metastasis in patients with FTC. Conclusions: The presence of a RAS mutation, especially a NRAS codon 61 mutation, was significantly associated with the distant metastasis. The NRAS codon 61 mutation status might be a potential prognostic factor in FTC patients.
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- 2014
30. Reference interval for thyrotropin in a ultrasonography screened Korean population
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Hyung-Don Kim, Soo Han Kim, Won Bae Kim, Hyemi Kwon, Min Ji Jeon, Yun Mi Choi, Yunkyoung Lee, Suyeon Park, Tae Yong Kim, Won Gu Kim, Eun Kyung Jang, Young Kee Shong, and Mijin Kim
- Subjects
Adult ,Male ,medicine.medical_specialty ,endocrine system ,Time Factors ,endocrine system diseases ,Cross-sectional study ,Thyroid Gland ,Physiology ,Thyrotropin ,Reference range ,Thyroid Function Tests ,Thyroid function tests ,Reference values ,Young Adult ,Sex Factors ,Predictive Value of Tests ,Internal medicine ,Republic of Korea ,Medicine ,Humans ,Endocrinology-Metabolism ,Family history ,Thyroid cancer ,Aged ,Ultrasonography ,medicine.diagnostic_test ,business.industry ,Thyroid disease ,Thyroid ,Smoking ,Case-control study ,Age Factors ,Middle Aged ,medicine.disease ,Thyroid Diseases ,medicine.anatomical_structure ,Endocrinology ,Cross-Sectional Studies ,Thyroid microsomal antibodies ,Case-Control Studies ,Female ,Original Article ,business ,hormones, hormone substitutes, and hormone antagonists ,Biomarkers - Abstract
BACKGROUND/AIMS The diagnostic accuracy of thyroid dysfunctions is primarily affected by the validity of the reference interval for serum thyroid-stimulating hormone (TSH). Thus, the present study aimed to establish a reference interval for TSH using a normal Korean population. METHODS This study included 19,465 subjects who were recruited after undergoing routine health check-ups. Subjects with overt thyroid disease, a prior history of thyroid disease, or a family history of thyroid cancer were excluded from the present analyses. The reference range for serum TSH was evaluated in a normal Korean reference population which was defined according to criteria based on the guidelines of the National Academy of Clinical Biochemistry, ultrasound (US) findings, and smoking status. Sex and age were also taken into consideration when evaluating the distribution of serum TSH levels in different groups. RESULTS In the presence of positive anti-thyroid peroxidase antibodies or abnormal US findings, the central 95 percentile interval of the serum TSH levels was widened. Additionally, the distribution of serum TSH levels shifted toward lower values in the current smokers group. The reference interval for TSH obtained using a normal Korean reference population was 0.73 to 7.06 mIU/L. The serum TSH levels were higher in females than in males in all groups, and there were no age-dependent shifts. CONCLUSIONS The present findings demonstrate that the serum TSH reference interval in a normal Korean reference population was higher than that in other countries. This result suggests that the upper and lower limits of the TSH reference interval, which was previously defined by studies from Western countries, should be raised for Korean populations.
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- 2014
31. Differentiating the location of cervical lymph node metastasis is very useful for estimating the risk of distant metastases in papillary thyroid carcinoma
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Won Bae Kim, Ki-Wook Chung, Min Ji Jeon, Dong Eun Song, Eun Kyung Jang, Jong Ho Yoon, Young Kee Shong, Ji Min Han, Tae Yong Kim, Won Gu Kim, Yun Mi Choi, and Suck Joon Hong
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,Multivariate analysis ,Endocrinology, Diabetes and Metabolism ,Disease ,TNM staging system ,Metastasis ,Thyroid carcinoma ,Endocrinology ,Internal medicine ,medicine ,Humans ,Thyroid Neoplasms ,Lymph node ,Retrospective Studies ,business.industry ,Thyroid ,Carcinoma ,Retrospective cohort study ,Middle Aged ,medicine.disease ,Carcinoma, Papillary ,medicine.anatomical_structure ,Thyroid Cancer, Papillary ,Lymphatic Metastasis ,Female ,business - Abstract
SummaryObjective A larger primary tumour size, greater numbers of cervical lymph node (LN) metastasis are associated with a higher risk of distant metastases in papillary thyroid carcinoma (PTC). However, the impact of the location of cervical LN metastasis on distant metastasis is controversial. The aim of this study was to evaluate the risk of distant metastases according to the tumour size and LN status in PTC patients, with special consideration of the LN metastasis pattern. Patients This retrospective cohort study reviewed 1700 classical PTC patients who underwent initial thyroid surgery at Asan Medical Center between 2000 and 2004. Results The cumulative risk of distant metastases was increased with the increase in tumour size and was significantly different according to the location of involved LNs by the tumour node metastasis (TNM) staging. The cumulative risk in pN1b group showed the steepest increasing pattern with the increase in tumour size compared with pN1a and pN0/Nx group. When we analysed the cumulative risk of distant metastases according to the number of involved LNs, patients with more than 20 involved LNs also had the steepest increase in the risk of distant metastases with the increase in tumour size. However, only the location of involved LNs, not the number of involved LNs, was associated with distant metastases in multivariate analysis. Conclusions We confirm that the location of involved LNs categories based on the TNM staging system is more useful than the number of involved LNs categories for estimating the risk of distant metastasis in PTC. Differentiating N1a disease from N1b disease is important for the follow-up and management of patients with PTC.
- Published
- 2013
32. Positive Cytology Findings and a Negative Histological Diagnosis of Papillary Thyroid Carcinoma in the Thyroid: Is It a False-Positive Cytology or a Disappearing Tumor
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Won Bae Kim, Ji Min Han, Min Ji Jeon, Tae Yong Kim, Dong Eun Song, Eun Kyung Jang, Young Kee Shong, Gyungyub Gong, Yun Mi Choi, Jung Hwan Baek, and Won Gu Kim
- Subjects
Thyroid nodules ,Pathology ,medicine.medical_specialty ,Clinical Thyroidology / Original Paper ,endocrine system diseases ,medicine.diagnostic_test ,business.industry ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Thyroid ,Thyroidectomy ,Neck dissection ,medicine.disease ,Malignancy ,body regions ,Thyroid carcinoma ,medicine.anatomical_structure ,Fine-needle aspiration ,Cytology ,Medicine ,business - Abstract
Background: It infrequently occurs that cytologic diagnosis of papillary thyroid carcinoma (PTC) cannot be confirmed by histology after surgery. This phenomenon may be a false-positive cytology or a true disappearing tumor. Objectives: We evaluated patients who had consistent findings of PTC at fine needle aspiration cytology (FNAC) and no evidence of PTC in surgical specimens. Methods: Positive cytology findings and a negative histological diagnosis of PTC in the thyroid was defined as thyroid nodules with FNAC findings of PTC prior to surgery and no evidence of malignancy on histological examination of surgically removed thyroids. We retrospectively reviewed patients who underwent fine needle aspiration (FNA) and thyroidectomy in Asan Medical Center from 2004 to 2012. Results: Six patients were found who fit the definition of positive cytology findings and a negative histological diagnosis of PTC in the thyroid. The FNAC diagnosis of 6 patients was ‘malignancy' suggesting PTC according to the Bethesda system. All patients underwent thyroidectomy with central neck dissection. Three patients had reactive changes after FNA due to needle passage. Among these 3 patients, 2 had pathologically confirmed metastatic PTC in dissected lymph nodes. These 2 patients could be defined as true disappearing PTC in the thyroid after FNA. The remaining 3 patients had neither histologic alterations nor evidence of PTC in the thyroid and lymph nodes specimens. Conclusions: Both disappearing PTC and a false-positive result of FNAC should be considered in patients with positive cytology findings and a negative histological diagnosis of PTC in the thyroid.
- Published
- 2013
33. Physiological and molecular characterization of a newly identified entomopathogenic bacteria, Photorhabdus temperata M1021
- Author
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Ihsan Ullah, Jae-Ho Shin, Jong Guk Kim, In-Jung Lee, Jong Hui Lim, and Eun Kyung Jang
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Salmonella ,Nematoda ,Sequence analysis ,Moths ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Microbiology ,chemistry.chemical_compound ,medicine ,Animals ,Pest Control, Biological ,biology ,Fatty Acids ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,Galleria mellonella ,Phenotype ,chemistry ,Larva ,MacConkey agar ,Micrococcus luteus ,Antibacterial activity ,Photorhabdus ,Bacteria ,Biotechnology - Abstract
The present study concerned the identification and characterization of a novel bacterial strain isolated from entomopathogenic nematodes collected from different regions in Korea. The bacterial isolate M1021 was Gramnegative, bioluminescent, and produced red colonies on MacConkey agar medium. A rod-shaped structure was confirmed by the electron micrograph. Fatty acid composition was analyzed by using the Sherlock MIDI system. The identification was further supported by 16S rDNA sequence analysis, which revealed 96-99% sequence homology with strains of Photorhabdus temperata. The location of the isolated strain of P. temperata in the phylogenetic tree was confirmed and it was named P. temperata M1021. P. temperata M1021 exhibited catalase, protease, and lipase activities when grown on appropriate media supplemented with respective substrates. The culture of P. temperata M1021 exhibited insecticidal activity against the larvae of Galleria mellonella and the activity was the highest after 3-4 days of cultivation with agitating at 28 degrees C under 220 rpm. Antibacterial activity was also observed against Salmonella Typhimurium KCTC 1926 and Micrococcus luteus KACC 10488.
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- 2012
34. Abstract 2042: Low Slit2 expression is associated with the aggressiveness of papillary thyroid carcinoma
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Won Gu Kim, Seonhee Lim, Won Bae Kim, MinJi Jeon, Young Kee Shong, Tae Yong Kim, and Eun Kyung Jang
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Cancer Research ,endocrine system diseases ,business.industry ,Thyroid ,Cancer ,Context (language use) ,medicine.disease ,Metastasis ,Thyroid carcinoma ,medicine.anatomical_structure ,Oncology ,ROBO1 ,medicine ,Cancer research ,Immunohistochemistry ,business ,Thyroid cancer - Abstract
Context: Slits, representative axon guidance molecules, and their Roundabout (Robo) transmembrane receptors are identified as key regulators of many cancers. One genome-wide liknage analysis suggested Slit-Robo Rho GTPase activating protein 1 gene as a candidate gene in papillary thyroid carcinoma (PTC) susceptibility. Objective: To identify the role of Slit-Robo signaling in PTC. Results : Slits(Slit 1-3) and Robos (Robo 1-4) were expressed in all of thyroid cancer cells and Slit2, Slit3, Robo1 and Robo4 presented higher expression than others. The mRNA expression of Slits and Robos was higher in fresh frozen PTC tissues than matched normal tissues. When we conducted immunohistochemical staining of Slit2 and Robo1 in thyroid tissues, the positivity of Slit2 and Robo1 were higher in PTC tissues than benign or normal tissues. However, negative Slit2 expression was significantly associated with cervical lymph node (LN) metastasis, distant metastasis and cervical recurrence of PTC (P Conclusions : Low expression of Slit2 was associated with poor prognostic factors in PTC. This present study demonstrated the potential of Slit2 as a novel prognostic and therapeutic factor in PTC. Citation Format: MinJi Jeon, Won Gu Kim, Seonhee Lim, Eun Kyung Jang, Tae Yong Kim, Young Kee Shong, Won Bae Kim. Low Slit2 expression is associated with the aggressiveness of papillary thyroid carcinoma. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2042. doi:10.1158/1538-7445.AM2015-2042
- Published
- 2015
35. Erratum: Figure Correction: Standardized Thyroid Cancer Mortality in Korea between 1985 and 2010
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Eun Kyung Jang, Young Kee Shong, Tae Yong Kim, Won Bae Kim, Won Gu Kim, Yun Mi Choi, Min Ji Jeon, and Hyemi Kwon
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lcsh:RC648-665 ,Information retrieval ,Bar chart ,Computer science ,Endocrinology, Diabetes and Metabolism ,medicine.disease ,computer.software_genre ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,law.invention ,Endocrinology ,law ,medicine ,Data mining ,Thyroid cancer ,computer - Abstract
Endocrinol Metab 2014;29:530-5 http://dx.doi.org/10.3803/EnM.2014.29.4.530 Corrections for Figure 2 in page 534 are needed. The bar graphs of male and female are drawn as same graphs. We apologize for any inconvenience that this may have caused.
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- 2015
36. Abstract 4009: N-ras codon 61 mutation is associated with distant metastasis in patients with follicular thyroid carcinoma
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So Young Sim, Dong Eun Song, Ji Min Han, Yun M. Choi, Won Bae Kim, Tae Yong Kim, Eun Kyung Jang, Young Kee Shong, Eui Young Kim, Won Gu Kim, and MinJi Jeon
- Subjects
Neuroblastoma RAS viral oncogene homolog ,Cancer Research ,Pathology ,medicine.medical_specialty ,Mutation ,business.industry ,Cancer ,medicine.disease_cause ,medicine.disease ,Metastasis ,Thyroid carcinoma ,Oncology ,Cancer research ,medicine ,HRAS ,KRAS ,Carcinogenesis ,business - Abstract
Distant metastasis is a main cause of death in follicular thyroid carcinoma (FTC) patients. Factors related to distant metastasis in FTC had been known as age, primary tumor size, and invasiveness. Ras mutations were also supposed to be associated with poor clinical outcomes. We analyzed Ras mutations in FTC with a distant metastasis (FTC M1, n=28), size matched-FTC without a distant metastasis (FTC M0, n=28), follicular adenoma (FA, n=17), and nodular hyperplasia (NH, n=12) to figure out the roles of Ras mutations in follicular thyroid carcinogenesis and metastasis. In addition, we assess the relationship between Ras mutations and clinical outcomes in FTC patients. NRAS, HRAS, and KRAS mutations were assessed using direct sequencing method. Among 85 patients, 39 patients (46%) had Ras mutations. NRAS codon 61 mutation (n=21; 25%) was the most common point mutation. HRAS codon 61, KRAS codon 12/13, and KRAS codon 61 mutations were found in 7, 6, and 4 patients, respectively. NRAS codon 12/13 mutation was found in only 1 patient, and HRAS codon 12/13 mutation was not found. Ras mutations were significantly more common in the FTC than FA or NH groups. Especially, NRAS codon 61 mutation was associated with distant metastasis in patients with FTC. However, there was no significant difference in survival between the Ras mutation positive-FTC and Ras mutation negative-FTC patients. Ras mutation, especially NRAS codon 61 mutation, was significantly associated with the presence of distant metastases. NRAS codon 61 mutation status might be a potential prognostic factor in FTC patients. Citation Format: MinJi Jeon, Eun Kyung Jang, Dong Eun Song, So Young Sim, Eui Young Kim, Yun Mi Choi, Ji Min Han, Won Gu Kim, Tae Yong Kim, Young Kee Shong, Won Bae Kim. N-ras codon 61 mutation is associated with distant metastasis in patients with follicular thyroid carcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4009. doi:10.1158/1538-7445.AM2014-4009
- Published
- 2014
37. A Case Report of Papillary Thyroid Carcinoma Presenting as Elevated Serum CA 19-9
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Won Bae Kim, Yun Mi Choi, Ho Cheol Kim, Eun Kyung Jang, Young Kee Shong, Tae Yong Kim, and Suck Joon Hong
- Subjects
Elevated serum ,Thyroid carcinoma ,medicine.medical_specialty ,Endocrinology ,business.industry ,Internal medicine ,medicine ,CA19-9 ,business - Abstract
본 증례는 수술 전 증가되었던 혈청 CA 19-9 농도가 갑상선전절제술 후 정상화되었고, 갑상선내 다른 병리 소견이 없어서 갑상선유두암 조직이 CA 19-9 분비의 원인이었음을 알수 있는 증례이다. 이는 혈청 CA 19-9 증가의 원인으로서 밝혀진 갑상선유두암의 국내 첫 증례 보고이며, 췌장 담도 질환이 동반되지 않은 환자에서 혈청 CA 19-9 증가가 있다면 갑상선암의 가능성도 고려해야 함을 시사한다.
- Published
- 2013
38. A Case Report of Bile Duct Duplication Arising from the Hepatic Hilum
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Seong Hee Ahn, Sung Koo Lee, Yil Sik Hyun, Yong Hoon Yoon, Eun Jeong Gong, Eun Kyung Jang, and Dae Wook Hwang
- Subjects
medicine.medical_specialty ,business.industry ,Bile duct ,Hilum (biology) ,medicine.disease ,Gallbladder Sludge ,Gastroenterology ,Curvatures of the stomach ,Ligamentum venosum ,medicine.anatomical_structure ,Biliary tract ,Internal medicine ,medicine.ligament ,Cholecystitis ,Medicine ,Pancreatitis ,Radiology ,business - Abstract
Anatomic variation in the hepatobiliary tract is relatively common, although bile duct duplication is rare. Detection of biliary tract variation is important because it is closely related to complications such as cholangitis, cholecystitis, pancreatitis, stone formation, and gastrointestinal tract malignancy. Therefore, preoperative imaging and a high index of suspicion are required to make an accurate diagnosis of a biliary tract anomaly. A 44-year-old woman presented with a 2-year history of epigastric pain after eating a meal and gallbladder sludge with chronic cholecystitis. The presumed diagnosis was gallbladder duplication. However, during surgery it was observed that the tubular structure was parallel with the fissure for the ligamentum venosum and ended at the lesser curvature of the stomach. The pathologic diagnosis was bile duct duplication. We report a case of a unique variant of bile duct duplication arising from the hepatic hilum. (Korean J Med 2012;82:465-469)
- Published
- 2012
39. A Case of Pulmonary Lymphangiomatosis
- Author
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Chang-Min Choi, Dong Soon Kim, Woo Sung Kim, Jin Woo Song, Sang Do Lee, Eun Kyung Jang, Tae Sun Shim, Yeon-Mok Oh, and Chang Rae Kim
- Subjects
medicine.medical_specialty ,Pleural effusion ,business.industry ,medicine.medical_treatment ,Mediastinum ,Chylothorax ,Thoracentesis ,Lung biopsy ,medicine.disease ,Thoracostomy ,Surgery ,medicine.anatomical_structure ,Lymphatic system ,medicine ,Radiology ,business ,Lymphangiomatosis - Abstract
Pulmonary lymphangiomatosis is a rare disorder involving the entire intrathoracic lymphatic system from the mediastinum to the pleura. Pulmonary lymphangiomatosis mostly occurs in children and young adults without gender predilection. Although it is pathologically benign, it shows a progressive and fatal course with variable initial presentation. We now report a case of pulmonary lymphangiomatosis in a 35-year-old man. He presented with hemoptysis 6 months previously. Chest x-ray and a chest computed tomography scan showed diffuse interstitial thickening with left pleural effusion. Chylothorax was confirmed by thoracentesis. Lymphangiography showed dilated and tortuous lymphatic channels. Surgical lung biopsy revealed proliferation of complex anastomosing lymphatic channels. He was diagnosed with pulmonary lymophangiomatosis. Closed thoracostomy and chemical pleurodesis were done and the dyspnea was reduced.
- Published
- 2011
40. Identification and Characterization of the Insecticidal Toxin 'Makes Caterpillars Floppy' in Photorhabdus temperata M1021 Using a Cosmid Library
- Author
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Ihsan Ullah, Eun-Kyung Jang, Min-Sung Kim, Jin-Ho Shin, Gun-Seok Park, Abdur Rahim Khan, Sung-Jun Hong, Byung-Kwon Jung, JungBae Choi, YeongJun Park, Yunyoung Kwak, and Jae-Ho Shin
- Subjects
insecticidal toxin ,Galleria mellonella ,makes caterpillar floppy ,Photorhabdus temperata ,Tenebrio molitor ,Medicine - Abstract
Photorhabdus temperata is an entomopathogenic enterobacterium; it is a nematode symbiont that possesses pathogenicity islands involved in insect virulence. Herein, we constructed a P. temperata M1021 cosmid library in Escherichia coli XL1-Blue MRF` and obtained 7.14 × 105 clones. However, only 1020 physiologically active clones were screened for insect virulence factors by injection of each E. coli cosmid clone into Galleria mellonella and Tenebrio molitor larvae. A single cosmid clone, PtC1015, was consequently selected due to its characteristic virulent properties, e.g., loss of body turgor followed by death of larvae when the clone was injected into the hemocoel. The sequence alignment against the available sequences in Swiss-Prot and NCBI databases, confirmed the presence of the mcf gene homolog in the genome of P. temperata M1021 showing 85% homology and 98% query coverage with the P. luminescens counterpart. Furthermore, a 2932 amino acid long Mcf protein revealed limited similarity with three protein domains. The N-terminus of the Mcf encompassed consensus sequence for a BH3 domain, the central region revealed similarity to toxin B, and the C-terminus of Mcf revealed similarity to the bacterial export domain of ApxIVA, an RTX-like toxin. In short, the Mcf toxin is likely to play a role in the elimination of insect pests, making it a promising model for use in the agricultural field.
- Published
- 2014
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