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Актуальність. Тривале паління тютюну дуже часто призводить до розвитку хронічного обструктивного захворювання легень і захворювань серцево-судинної системи. Мета — визначення частоти і ступеня порушень дихальної функції в пацієнтів з артеріальною гіпертензією (АГ) залежно від стажу тютюнопаління. Матеріали та методи. Під наглядом перебували 80 пацієнтів із встановленим діагнозом «артеріальна гіпертензія I–III ступеня без обструктивного захворювання легень в анамнезі». Контрольну групу становили пацієнти з АГ, які ніколи не курили (n = 52), основна група представлена хворими, які палили в минулому або палять на сьогодні (n = 28). У всіх пацієнтів було проведено збір анамнестичних даних і скарг, аналіз антропометричних даних, індексу куріння, даних офісного і домашнього моніторування, показників вентиляційної функції легень. Результати. За отриманими результатами, у пацієнтів з АГ та тютюнопалінням у анамнезі ≥ 10 пачко/років вірогідно частіше відмічалися порушення функції дихання (11,3 %, p < 0,05), ніж у пацієнтів, які не палять (лише 3,8 %). При аналізі кореляційних відносин було встановлено вірогідний обернений зв’язок між стажем тютюнопаління ≥ 10 пачко/років та показником співвідношення обсягу форсованого видиху за першу секунду до форсованої життєвої ємності легень: у чоловіків r = –0,8052 (p < 0,01), у жінок r = –0,69154 (p < 0,01). Висновки. Для своєчасного виявлення супутньої патології та призначення адекватної терапії на етапі первинної медичної допомоги можна пропонувати проводити вимірювання показників функції дихання у всіх пацієнтів-курців з артеріальною гіпертензією та стажем куріння в анамнезі більше 10 пачко/років.

The problem of diagnosis and effective treatment of one of the most common complications of dental caries, such as pulpitis, including pediatric patients, is still quite relevant at the present time. Considering anatomy-physiological and age-related features of temporary and permanent teeth in children, the rapid and aggressive course of the carious process in poorly mineralized hard tissues due to the untimely and incomplete “maturation” of them, it is beyond any doubts that scientists and practitioners involved in pediatric dentistry deal with such complications in the clinic much more often than when dealing with adults. A particularly risky group are those who develop decompensated caries, according to classification by T.F.Vinogradova. Therefore, the search for more advanced methods of diagnosis and treatment of both acute and chronic inflammation of pulp, especially with the preservation of its viability, is logical and relevant in modern dentistry. Existing methods for the biological treatment of pulpitis have their positive aspects, but possible complications up to necrosis or pulp gangrene in remote terms indicate the need for improvement both the choice of a more effective method with clear indications for its use, and the technology and material of odontotropic direction. The aim of the work was to study the results of treatment of pulpitis in experimental animals by using tricalcium silicate cement for its further application in the clinic. An experimental study was conducted on 18 animals (9-month-old male rabbits, Dutch breed) in vivarium setting to obtain information on the morphogenetic mechanisms of regeneration of living tissues and cells of dental pulp in which traumatic pulpitis was induced artificially. By the nature of odontotropic material, four groups of experimental animals were divided into: group I (control) – traumatic pulpitis was treated without special odontotropic paste, group II - treatment of pulpitis with material based on calcium hydroxide, group III - material with trioxide aggregate, group IV - material with tricalcium silicate. Every two, four, and six weeks from the moment the animals were introduced into the experiment, the damaged teeth were removed and treated in a certain way and, after special treatment, were amenable to histological examination. In the pulp tissue, the state of its main components was studied: stroma, cellular elements and blood vessels of the microvasculature. After excretion of animals from the experiment on the 2nd and 6th weeks tissues of tooth were fixed in 10% formalin. Fixed in formalin tissue has been routine proceeding after decalcification in acetic acid with making histological slides which were stained with hematoxylin and eosin (H&E), according to van Gieson, Mallory, PAS-reaction was performed. The slides were studied with the microscope “Olympus BX-41” and followed interpretation by “Olympus DP-soft version 3.2”, which was used for morphometric study. Statistical comparison was performed using Mann-Whitney test for statistical analysis. The accepted level of significance was p

У статті наведені результати досліджень з вивчення впливу комплексного біорегуляційного препарату Траумель С [32], який застосовували у вигляді ін’єкцій, на клінічну ефективність терапії гострого синуситу в дітей на фоні базисного лікування. Оцінка ефективності терапії проводилася шляхом визначення інтегральних гематологічних індексів, розрахованих на основі показників клінічного аналізу крові, та маркерів запалення (загальний білок та його фракції, С-реактивний білок). Включення до схем терапії препарату Траумель С у пацієнтів з гострим синуситом показало вірогідно швидше зниження лейкоцитарних індексів інтоксикації та завершення запалення. Даний препарат ефективний і добре переноситься пацієнтами.

The article shows the results of physical therapy of those who have had coronavirus infection COVID-19 and who have completed a month's course of physical rehabilitation as a part of rehabilitation measures in the conditions of the innovative clinic "Academy of Health". The aim of the research is to study the impact of pulmonary rehabilitation programs for patients with moderate to severe COVID-19. The implementation of special breathing exercises made it possible to improve the oxygen saturation in the blood, to reduce shortness of breath and indicators of respiratory symptoms. The tolerance to exercise has increased; physical activity and the quality of life of patients with pneumonia associated with COVID-19 have improved. The best results of the EQ-5D quality of life assessment were recorded in the experimental group compared to the control group.

Voloshyna Olena, Samorukova Viktoriia, Dychko Tetiana, Balashova Iryna, Naidyonova Olena, Chaika Anna, VoloshynAndrii, Gushcha Sergey. Adherence to home blood pressure monitoring depending on the frequency of blood pressure measurements during the day. Journal of Education, Health and Sport. 2020;10(3):34-41. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.03.003 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.03.003 https://zenodo.org/record/3709240 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2020; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 20.02.2020. Revised: 25.02.2020. Accepted: 13.03.2020. Adherence to home blood pressure monitoring depending on the frequency of blood pressure measurements during the day Voloshyna Olena1, Samorukova Viktoriia1, Dychko Tetiana1, Balashova Iryna1, Naidyonova Olena1, Chaika Anna1, VoloshynAndrii1, Gushcha Sergey2 1Odessa National Medical University, Ukraine 2State Institution «Ukrainian Research Institute of Medical Rehabilitation Therapy of Ministry of Health of Ukraine», Odessa, Ukraine Abstract The article analyzes the adherence to home blood pressure monitoring (HBPM) with different measurement frequency during the day in 90 patients with hypertension. It was found that after 2 weeks of blood pressure monitoring the greatest was patients' adherence to blood pressure measurement at home once (96.7%) or 2 times a day (90.0%), the lowest - at home pressure measurement every 2– 3 hours during the day (76.6%).The adherence to frequent HBPM (2-3 hours during the day) appeared to be significantly less compared to HBPM once or twice daily, due to more frequent violations of HBPM. Regardless of the frequency of measurements at HBPM, adherence to monitoring is most commonly impaired in working men, compared with women of similar age and employment. Key words: arterial hypertension, adherence to blood pressure, blood pressure.

The research work deals with the actual problem of visual impairment in children at an early age, which creates difficulties in the accumulation of sensory experience, which delays the formation of mental processes. Children with visual impairments have specific peculiarities of activity, communication and psychophysical development, which are manifested in lagging, impairment and originality of the development of motor activity, spatial orientation, the formation of ideas and concepts, in the methods of object and practical activity, in the peculiarities of the emotional and volitional sphere, social communication, integration into society, adaptation to work. The authors developed a rehabilitation complex of physical exercises and studied the effectiveness of its influence on the psychophysical status of children and adolescents at the age of 10-16 years with visual impairments. In the course of the study (groups of children with visual impairment at the age of 10-14 and 15-16 years), it was found that the author's rehabilitation complex of physical exercises has a positive effect on the performance of tests characterizing the psychomotor reaction to a simple auditory and motor reaction, the sound version of the corrective test , contact coordination, hand strength and endurance, tapping test (strength of the nervous system) in children with visual impairment, and it can be recommended for widespread use by specialists to work with children at the age of 10-16 years with visual impairments to improve the psychophysiological state and improving the quality of life.

The article is devoted to the study of indicators of function of the autonomic and cardiorespiratory systems in children. A study was conducted of 43 children of 11-14 years, including 19 children with scoliosis, and 24 practically healthy peers. The purpose of the study was to study the dynamics of individual indicators of the function of the autonomic nervous and cardiorespiratory systems of children aged 11-14 years with scoliosis compared with their almost healthy peers from secondary school. Analysis of the dynamics of the test of physical performance of children aged 11-14 years showed that the need for myocardium in oxygen both during rest and during exercise, as well as the first and second rest was the same among practically healthy children and boys and girls with scoliosis. During physical activity, myocardial oxygen demand increased sharply and equally both among girls and boys of 11-14 years of age as in the control group and in experimental control group without significant gender difference. There were no gender differences in the Kerdo index in the group of almost healthy adolescents of 11-14 years and adolescents with scoliosis of the same age. However, when comparing between observation groups, one immediately draws attention is that several times exceeding the value of the Kerdo index in the group of children with scoliosis, and this pattern is characteristic for both girls and boys. The determined fact indicates that the extremely powerful activation of the sympathetic nervous system is found in children with scoliosis also persists in adolescence. During physical activity the same tendency persists in the distribution of the parameter of the Kerdo index: a significant excess of the index in the group of girls and boys aged 11-14 years with scoliosis compared with the control group. Determination of the degree of training of the cardiovascular system on the basis of the calculation of the endurance coefficient allowed us to establish that the corresponding coefficient was not significantly different among the groups under study in the state of rest and physical activity, as well as the first and second rest from the physical activity. Analysis of the systolic heart rate according to the Robinson index showed that in the rest state the highest index was found in almost healthy adolescent girls, although statistically significant difference was found only among the girls of the experimental and control groups. The best energy potential of the body of adolescents with scoliosis in comparison with almost healthy adolescents persists since childhood. The Robinson index increased while physical activity, with a statistically significantly higher index in the experimental group of girls compared to male adolescents. The same tendency was found both during the first and the second rest. The highest maximum stroke volume of blood at rest was found in adolescent girls with scoliosis. Moreover, this indicator statistically significantly exceeded both the corresponding value in boys with scoliosis and the stroke volume of blood in almost healthy boys and girls. The value of minute volume of blood at rest and during the first and second rest was not significantly different among almost healthy children aged 11-14 years compared with their peers with scoliosis. During physical exercises a statistically significant lower value of minute blood volume was observed only in adolescent girls with scoliosis compared to adolescent boys in the study group. Conclusions. Thus in the result of our study it was found that children with scoliosis are behindhand their almost healthy peers in terms of autonomic nervous function and cardiorespiratory systems. Myocardial oxygen demand at rest, during physical activity and during restitution was lower in scoliosis children than in practically healthy ones. The Kerdo index in children with scoliosis before, during and after exercises was higher than in practically healthy ones. Children with scoliosis had lower Robinson’s index. Prospects for further research were to study the dynamics of autonomic nervous and cardiorespiratory function in adolescents aged 15-17 years with scoliosis compared with their relatively healthy peers from secondary school.

Introduction. For today, the treatment of patients with comorbid pathology arterial hypertension (AH) and chronic obstructive pulmonary disease (COPD) remains insufficiently effective. The reason for this may be not only mutually aggravating course of these diseases, but also violations of adherence to antihypertensive and bronchodi-lator therapy in this category of patients. Aim. To determine the effectiveness of patient – oriented correction of adherence to treatment in patients with comorbid pathology arterial hypertension and chronic obstructive pulmonary disease after determining and elimi­nating the main causes of violations of adherence to antihypertensive (AHT) and bronchodilator treatment (BDT). Materials and methods. 94 patients with the established diagnosis of stage II hypertension and COPD of the 2nd or 3rd clinical groups (groups B and C) were examined, divided into two groups: the main group – 51 patients (54.3 %) with hypertension and concomitant COPD and comparison group – 43 patients (45.7 %) with essential hypertension. The data were analyzed by office and home blood pressure monitoring, spirometry parameters, the results of the questionnaires mMRS (modified Medical Research Council), CAT (COPD Assessment Test) and D. E. Morisky – L. W. Green’s commitment to the treatment. Results. It was found that in patients with AH and concomitant COPD, in addition to the significant reduction in overall adherence to treatment, low attachment to bronchodilator therapy is more often than antihypertensive: 84.3 ± 5.1 % and 64.7 ± 6.7 % respectively (p < 0.02). Conducting the patient-oriented correction of adherence to treat­ment leads to significantly better control of blood pressure in patients with hypertension associated with COPD – in 73.1 ± 6.9 % of COPD patients in clinical group B and only in 57.6 ± 5.3 % of patients with clinical group C (p < 0,02) and improves the function of external respiration, including FEVj/FVC at 9.4 ± 4.1 % (p < 0,05). Conclusions. In patients with comorbid pathology of AH and COPD, in addition to the significant reduction in overall adherence to treatment, low bronchodilator tolerance is significantly more common than antihypertensive therapy. Conducting the patient-oriented correction of adherence to treatment leads to significantly better control of blood pressure in patients with hypertension associated with COPD and improves the function of external respiration.

Introduction. The prevalence of arterial hypertension in Ukraine reaches 35.0 % among the adult population. The frequency of cases of resistant hypertension in the population of patients with hypertension varies from 5.0 to 18.0 %. In the structure of resistant arterial hypertension, pseudoresistant is observed in 90.0-95.0 % of cases. Aim. To compare the various methods of the differential diagnosis of essential resistant and pseudoresistant arterial hypertension in the practice of a doctor of general practice. Materials and methods. 120 patients with uncontrolled essential arterial hypertension, taking three antihypertensive drugs, and randomly divided into two groups – the main group – 60 patients (26 (43.3%) women and 34 (56.7 %) men, middle age 54.6 ± 9.2 years) and comparison group – 60 patients (24 (40.0 %) women and 36 (60.0 %) men, middle age 55.4 ± 8.5 years) were examined. The study consisted of three stages. At the first stage, the differential diagnosis of essential resistant and pseudoresistant arterial hypertension, using the method proposed by us for the patients from the main group was performed. Subsequently, the patients with detected by our acute pharmacological test pseudoresistant arterial hypertension continued to participate in the study and to take three previously assigned antihypertensive drugs in maximal tolerated doses in order to evaluate the sensitivity and specificity of our method and to verify the authenticity of pseudoresistant arterial hypertension by the traditional method. At the second stage, the differential diagnosis of essential resistant and pseudoresistant arterial hypertension using the traditional method for the patients from the comparison group was performed and the frequency of resistant and pseudoresistant arterial hypertension in both groups was determined. In the third stage, the assessment of the parameters of office blood pressure in the patients of both groups was performed on each visit. Results. Based on the conducted research, the effectiveness of the new method of differential diagnosis of essential resistant and pseudoresistant arterial hypertension in the reduction of the timing of the diagnosis of pseudoresistant arterial hypertension and the faster achievement of blood pressure control in this category of patients is scientifically substantiated. Conclusions. The sensitivity of the proposed method of differential diagnosis of essential resistant and pseudoresistant arterial hypertension, in comparison with the traditional one, is 95.7 % (95% CI 91.8-99.6), the specificity is 57.2 % (95% CI 47.5-66.9), the accuracy index (diagnostic value of the test) is 86.7 % (95% CI 80.1-93.3), compared to the traditional one. The frequency of pseudoresistant arterial hypertension detection in the comparison group of patients was 80.0 %, in the main group – 88.0 %, the incidence of resistant arterial hypertension was 20.0 and 12.0 %, respectively = 1.7; df = 1; p > 0.05). The application of the developed by us method of differential diagnosis of essential resistant and pseudoresistant arterial hypertension can significantly reduce the timing of differential diagnosis and accelerate the timing of the diagnosis of pseudoresistant arterial hypertension – to 3.9 і 2.6 days, compared with the traditional method – 16.4 і 6.8 days (p < 0.05), that allows to achieve the significant reduction of systolic blood pressure (>10.0% of the baseline level) on average 4.5 і 1.5 days earlier than using the traditional method – 20.2 і 3.6 days (p < 0.05).

Розглянуто задачу структурно-параметричного синтезу гібридної нейронної мережі на основі використання топології багатошарового персцептрону. Гібридизація досягається за рахунок використання штучних нейронів різних типів, а саме Q-нейрону,W-нейрону і класичного нейрону. Розв’язано задачу оптимального вибору кількості шарів, нейронів в шарах, а також типів нейронів у кожному шарі та принципи їх чергування за допомогою генетичного алгоритму SPEA2. Наведено приклади побудови гібридної нейронної мережі за даною методологією та заданим критерієм оптимізації для розв’язання задач класифікації та прогнозування. The problem of structural-parametric synthesis of hybrid neural network based on the use of multilayer perceptron topology is considered. Hybridization is achieved through the use of artificial neurons of different types, namely Q-neuron, W-neuron and classical neuron. The problem of optimal selection of the number of layers, neurons in layers, as well as the types of neurons in each layer and the principles of alternating them using the genetic algorithm SPEA2 is solved. Examples of building a hybrid neural network using this methodology and a given optimization criterion for solving classification and forecasting problems are given.

Human health is largely determined by the level of physical development and functionality of the body, the foundations of which are laid in childhood. Currently, social and environmental conditions, difficulties in organizing health care have led to an increase in all types of morbidity. Indicators of physical development decrease, the number of children with manifestations of acceleration of development decreases, at each fourth child the posture disturbance is noted. The disability of children is growing. Individuality of physical development and functional state of the body of children (boys and girls) certainly exists, it is the basis of well-known clinicians situations such as “paradoxical” reactions of the body, differences in the clinical picture of the disease at the same dose of infectious agent, abnormal effects in individuals and are referred to by clinicians as “individual features of the reactivity of the organism.” Based on this, in our work we use and present individual indicators of physical development (indicators of anthropometric status, external respiration, physical development and functional status) of the body of children with visual pathology, boys and girls of different ages. The physical condition of a person determines the degree of endo- and exointoxication and immunological reactivity. Therefore, the study of the impact of a new set of rehabilitation measures on immuno-hematological parameters that characterize the overall immunological reactivity of children aged 7-10 years with visual pathology is necessary to address its possible use. The aim of the study was to study the effect of the developed method of physical rehabilitation on the immunological reactivity of the body of children aged 7-10 years with visual pathology. Object and methods of research. Approbation of the complex method of physical rehabilitation carried out at children (31 children) aged 7-10 years of comprehensive special boarding school of I-III degrees № 23 (director Kotlyarov MV) Slavyansk under the control of medical workers of institution who carried out peripheral blood collection for laboratory examination of children on a hematology analyzer of the HB series, which was discussed in the previous section. Carrying out of rehabilitation measures of the method developed by us which is directed on improvement of physical development of these children and improvement of immunological reactivity of an organism of children, aged 7-10 years, with pathology of sight. For the rehabilitation of children with visual pathology of 7-10 years, we used the method of exercise for these children, developed plans based on the sex of deep disorders, and so on. The stage of the study of the use of this method in children with visual pathology 7-10 was found to influence the effects of rehabilitation measures on the immunological reactivity of the organism. First, the definitions summarize the impact on all adolescents with visual pathology, and then separately on boys and girls depending on age. Research results and their discussion. In children aged 7-10 years with visual pathology, who underwent a set of rehabilitation measures, the value of the index of the ratio of agranulocytes and ESR increases by 39.16%, which is evidence of stimulation of adaptive immune activity. This reduces the value of lymphocyte-granulocyte index by 12.20%, which confirms the activation of granulocyte leukocytes – neutrophilic polymorphonuclear leukocytes and eosinophils. Along with this, there is a tendency to decrease (P> 0.05) the index of immunological reactivity – by 10.71%, nonspecific resistance – by 12.20%, lymphocyte-granulocyte index – by 10.21%, the index of the ratio of lymphocytes and monocytes – at 11.49%. These changes in immuno-hematological parameters indicate that the rehabilitation measures in children aged 7-10 years with visual pathology, positively and slowly affect the overall immunological reactivity of the body of children with visual pathology. There is no immuno-hematological indicator that would indicate a negative impact on the physical development of the body and a significant decrease in immunohematological parameters that characterize the functional and immune status of children with visual pathology. Conclusions. Thus, the development of a set of rehabilitation measures for both boys and girls aged 7-10 years with visual pathology, improves immune and hematological parameters that characterize the state of nonspecific anti-infective and specific immune protection (immunological reactivity) of children aged 7-10 years with visual pathology. No changes in immuno-hematological parameters in these children, which could be a contraindication to the use of new sets of rehabilitation measures. Prospects for further research. The main scientific provisions obtained and presented in the article are the basis for studying the impact of measures and tools aimed at improving the state of non-specific anti-infective and specific immune protection (immunological reactivity) of children with visual pathology aged 10-14 years.

The syndromes of spasticity and chronic pain are diagnosed in the majority of patients in different periods of recovering from spinal injury. Current synthetic or semi-synthetic matrixes, tissue and cell transplants, which are used in the treatment of spinal cord injuries, can affect the development of the syndrome of spasticity and chronic pain.Objective. To examine the effect of fetal cerebellum tissue transplantation (FCTT) on the course of the spasticity and chronic pain syndrome after experimental spinal cord injury.Materials and methods. Animals – albino outbred male rats (5.5 months, 300 grams, inbred line, the original strain – Wistar); main experimental groups: 1 – spinal cord injury only (n = 16), 2 – spinal cord injury + immediate homotopical implantation of a fragment of the fetal cerebellum tissue (n = 15). Model of injury – left-side spinal cord hemisection at Т11 level; verification of spasticity – by Ashworth scale and electroneuromyography, severe pain syndrome – by autophagy.Results. FCTT does not affect the frequency of severe neuropathic pain syndrome, is accompanied by early (1st week) debut of spasticity signs, significantly increases the level of spasticity (1st-3rd weeks), which is most likely due to glutamatergic effect of descendants of immature transplant cells – cerebellar granular neurons precursors. The maximum increase of the spasticity in the case of FCTT was observed at the 3rd week, in the control group – within the 1st and 4th weeks; from the 4th week after FCTT till the end of the experiment stabilization of spasticity rate in the range of 1.8-2.1 points was observed, which is probably due to the autoimmune motoneurons loss in the perifocal area. At the 24th week the level of spasticity in the case of FCTT succumbed to 2.1 ± 0.3 points, in the control group – 2.6 ± 0.4 Ashworth’s points (p > 0.05).Conclusion. Immediate fetal cerebellum tissue transplantation in rats with spinal cord injury causes early pro-spastic effect, in the long term – stabilizes spasticity level.

Актуальність. Відкрита проникна травма з потраплянням стороннього тіла в хребтовий канал є одним з найбільш поширених уражень спинного мозку (СМ) військового часу; експериментальне відтворення окремих складових багатокомпонентної взаємодії між стороннім тілом та СМ утруднене.Мета дослідження. Вивчити клініко-морфологічні особливості моделі зазначеного виду спінальної травми.Матеріали і методи. Тварини — білі щури-самці (вік 5,5 міс, маса тіла 300 г, інбредна лінія, похідна породи Wistar); експериментальні групи: основна (травма СМ + негайна гомотопічна імплантація фрагмента мікропористого гідрогелю — стороннє тіло [n=10]); групи порівняння (травма СМ [n=16], травма СМ + імплантація хімічно ідентичного макропористого гідрогелю NeuroGelTM [n=20]). Модель травми — лівобічне пересічення половини СМ на рівні ТXI; моніторинг функції задніх кінцівок за шкалою ВВВ; морфологічне дослідження — стандартні гістологічні методики; трансмісійна електронна мікроскопія.Результати. Компресія СМ біологічно сумісним пружнім стороннім тілом суттєво погіршує перебіг процесів регенерації; протягом перших 8 тиж показник функції (ПФ) задньої іпсилатеральної кінцівки (ЗІК) у тварин найнижчий — (1,30±0,94) бала за шкалою ВВВ, через 3–4 міс ПФ ЗІК статистично значуще збільшувався до (2,35±0,95) бала за шкалою ВВВ, що, найімовірніше, пов’язане з змінами форми стороннього тіла та його утилізацією, зменшенням тиску на СМ. Через 24 тиж спостереження ПФ ЗІК становив (8,45±0,92) бала (в групі NeuroGelTM) та (2,35±0,95) бала (в групі стороннє тіло). Стороннє тіло протягом періоду експерименту, на відміну від фрагментів NeuroGelTM, не інтегроване у тканину СМ, оточене щільною фіброзною капсулою, практично не інфільтроване тканинним компонентом. Морфологічні зміни у протилежній частині СМ на рівні ураження не виявлені.Висновок. Використана модель задовільно відтворює механічний компонент дії стороннього тіла на тканину СМ, ознаки синдрому посттравматичної спастичності; зменшення компресії СМ навіть у віддаленому періоді травми суттєво покращує процеси регенерації., Background. Penetrating spinal cord injury with a foreign body in the spinal canal is one of the most common spinal cord injuries during wartime; the experimental reproduction of particular elements of complex interaction between a foreign body and the spinal cord is complicated.Objective. To examine clinical and pathomorphological features of the model of this type of spinal cord injury.Materials and methods. Animals: albino male rats (5.5 months, 300 grams, inbred line, the original strain is Wistar); experimental groups: basic (spinal cord injury + immediate homotopical implantation of a fragment of the microporous hydrogel – a foreign body [n=10]); comparison groups (spinal cord injury [n=16], spinal cord injury + immediate homotopical implantation of chemically identical macroporous hydrogel NeuroGel™ [n=20]). Model of injury: left-side spinal cord hemisection at ТXI level; monitoring the function of hind legs — the BBB scale; pathomorphological study: conventional histological techniques, transmission electronic microscopy.Results. Compression of the spinal cord by biologically compatible foreign body significantly worsens the course of the regeneration process; during the first 8 weeks the hind ipsilateral leg function indicator (HI LFI) in animals was the lowest one — (1.30±0.94) points by BBB scale; during the 3rd–4th month HI LFI increases to 2.35±0.95 points by BBB scale, which is likely due to the change in the form of a foreign body and its utilization, decrease of the pressure on the spinal cord. On the 24th week of the follow-up HI LFI was (8.45±0.92) points (in NeuroGelTM group) compared with (2.35±0.95) points by BBB scale (in the group with a foreign body). During the experiment a foreign body, unlike the fragments of the NeuroGelTM, was not integrated into the tissue of the spinal cord, was surrounded by a thick fibrous capsule, hardly infiltrated by tissue component. Morphological picture in the contra-lateral part of the spinal cord at the level of injury did not change.Conclusion. The model satisfactorily a mechanical component of a foreign body effect on the spinal cord tissue, presents the picture of post-traumatic syndrome of spasticity; reducing the spinal cord compression even at the late period of injury significantly improves the regeneration process., Актуальность. Открытая проникающая травма с попаданием инородного тела в позвоночный канал является одним из наиболее распространенных повреждений спинного мозга (СМ) военного времени; экспериментальное воспроизведение отдельных составляющих многокомпонентного взаимодействия между инородным телом и СМ затруднено.Цель исследования. Изучить клинико-морфологические особенности модели приведенного вида спинальной травмы.Материалы и методы. Животные — белые крысы-самцы (возраст 5,5 мес, масса тела 300 г, инбредная линия, производная породы Wistar); экспериментальные группы: основная (травма СМ + немедленная гомотопическая имплантация фрагмента микропористого гидрогеля — инородное тело [n=10]); группы сравнения (травма СМ [n=16], травма СМ + имплантация химически идентичного макропористого гидрогеля NeuroGelTM [n=20]). Модель травмы — левостороннее пересечение половины СМ на уровне ТXI; мониторинг функции задних конечностей (по шкале ВВВ); морфологическое исследование — стандартные гистологические методики; трансмиссионная электронная микроскопия.Результаты. Компрессия СМ биологически совместимым упругим инородным телом существенно ухудшает течение процессов регенерации; в сроки до 8 нед показатель функции (ПФ) задней ипсилатеральной конечности (ЗИК) у животных наименьший — (1,30±0,94) балла (по шкале ВВВ); через 3–4 мес ПФ ЗИК статистически значимо увеличивался до (2,35±0,95) балла (по шкале ВВВ), что, вероятно, обусловлено изменением формы инородного тела, уменьшением его объема, давления на СМ. Через 24 нед наблюдения ПФ ЗИК составил (8,45±0,92) балла (в группе NeuroGelTM) и (2,35±0,95) балла (в группе инородное тело). Инородное тело в период эксперимента, в отличие от фрагментов NeuroGelTM, не интегрировано в ткань СМ, окружено плотной фиброзной капсулой, практически не инфильтрировано тканевым компонентом. Морфологические изменения в противоположной доле СМ на уровне повреждения не выявлены.Выводы. Апробированная модель удовлетворительно воспроизводит механический компонент влияния инородного тела на ткань СМ, признаки синдрома посттравматической спастичности; уменьшение компрессии СМ даже в отдаленном периоде травмы существенно улучшает процессы регенерации.

Fetal cerebellar tissue contains the largest number of neurogenic progenitors committed on the differentiation into glutamatergic neurons that can be used in the development of promising new treatment for spinal cord injuries.To evaluate the effect of fetal cerebellar tissue transplantation (FСTT) on the restoration of motor function after spinal cord injury in experiment.Materials and methods. Animals: inbred albino Wistar rats (5.5 months males, weighting 300 grams); main experimental groups: 1 – spinal cord injury + transplantation of a fragment of fetal (E18) rat cerebellum (n = 15), 2 – spinal cord injury only (n = 40). Model of an injury – left-side spinal cord hemisection at Т11; monitoring of the ipsilateral hind limb function (IHLF) – the Вasso-Вeattie-Вresnahan (BBB) scale.Results. FСTT normalizes the distribution of IHLF values, distorts the dynamics of the motor function recovery, transforming it from a progressive (in a control group) to the constant with variation within 3-3.6 points BBB during the experiment. FСTT causes early temporary positive effect on the functional state of the motor system, probably provided by mediator-dependent, neuroprotective, proangiogenic effect and remyelination. In our view, the gradual depletion of the FСTT positive effect due to resorption of the graft within the first 2 months is compensated by autoregenerative neoplastic process that is typical for the control group and by autoimmune utilization of myelin-associated inhibitors of axonal growth in the zone of injury that causes stability of the IHLF value during the observation period.Conclusion. Transplantation of fetal cerebellar tissue causes a short-term positive effect on the motor function recovery limited by the 1st month of the traumatic process. Evaluation of such type of neurotransplantation effectiveness requires taking into account the dynamics of the spasticity and chronic pain.

The aim of this study is improvement of the treatment of children with acute bronchitis using complex bio-regulatory drug Traumeel S on background of standard therapy. Materials and methods. 50 patients diagnosed with acute bronchitis aged 7 to 18 years were divided into two equal groups of 25 children. In the study group patients received, in addition to standard therapy, the drug Traumeel S, intramuscular injections, age-specific dose 1 time per day during 7 days, daily, followed by another 3 injections once per two days. The children of comparition group received standard therapy. To assess the dynamics of inflammation and organism reactivity were used proteinogram, shift of leucocytes index (SLI) and lymphocytic index (LI). Results. In the group which received Traumeel S SLI and LI significantly changed towards normal rates at the 7th day, while in the comparison group significant changes were recorded only after 14 days of treatment. The levels of α-2 globulin was significantly different (r

Актуальність. Відновлення функції спинного мозку (СМ) при спінальній травмі пов’язують з прогресом нейроінженерії та роботичної біоніки. Його результативність зменшується внаслідок виникнення синдрому спастичності та хронічного больового синдрому. Вивчення їх перебігу на тлі нейроінженерних втручань потребує застосування відповідних моделей спінальної травми.Мета. Вивчити зміни нервово-м’язового апарату, особливості перебігу синдрому спастичності, хронічного больового синдрому на моделі лівобічного поперечного пересічення половини (ЛПП) СМ щура в нижньогрудному відділі.Матеріали і методи. Електронейроміографія (ЕНМГ): пряма стимуляція СМ (амплітуда М-хвилі), реєстрація Н-рефлексу (співвідношення амплітуди Н-хвилі та М-хвилі — Н/М, %); верифікація спастичності за шкалою Аshworth; визначення екстероцептивної чутливості — за методом фон Фрея в модифікації Вейнштейна; функції задніх кінцівок — за шкалою Basso, Beattie, Bresnahan (ВВВ).Результати. Максимальна амплітуда М-хвилі у досліджуваному м’язі задньої іпсилатеральної (щодо зони травми) кінцівки (ЗІК) на 7-му тижні після ЛПП відповідала такій в інтактних тварин з дисоціацією показника функції (ПФ) (2,5 та 21 бал за шкалою ВВВ); до 23-го тижня — достовірно зменшувалася. Н/М у задніх кінцівках інтактних тварин (n=14) становило у середньому (34,14±3,17)%, у ЗІК (n=10) — (52,44±7,27)% (р, Relevance of the topic: Spinal cord function recovery upon spinal cord injury is associated with the progress of neuroengineering and robot bionics. The effectiveness is advanced by the development of syndrome of spasticity and chronic pain. The study of their passing under the condition of neuroengineering interventions requires the involvement of appropriate models of spinal injury.Objective: To examine changes in the neuromuscular system, peculiarities of development of spasticity syndrome and chronic pain syndrome on the model of lower thoracic left-side rat’s spinal cord hemisection (LHS).Materials and methods: Eleсtroneuromyography (ENMG): direct stimulation of the spinal cord (M- waves amplitude), registration of Hoffmann-reflex (the ratio of H-wave amplitude to M-wave amplitude — N/M, percent); verification of spasticity according to Ashworth scale; exteroceptive sensitivity — the method of von Frey in the modification of Weinstein; hindlimb function — Basso–Beattie–Bresnahan (ВВВ) scale.Results. The average value of the maximum amplitude of M-wave in the examined ipsilateral hindlimb (IH) muscle on the seventh week after LHS is equal to the indicator of intact animals with the dissociation of values of the function indicator (2.5 versus 21 points under the BBB scale); up to the 23rd week a reliable reduction of the indicator is observed. N/M for hindlimbs of intact animals (n=14) is 34,14±3,17 percent, for IH (n=10) is 52,44±7,27 percent (p, Актуальность. Восстановление функции спинного мозга (СМ) при спинальной травме связывают с прогрессом нейроинженерии и роботической бионики. Его результативность уменьшается вследствие возникновения спастичности и хронического болевого синдрома. Изучение их течения на фоне нейроинженерных вмешательств требует применения соответствующих моделей спинальной травмы.Цель. Изучить изменения нервно-мышечного аппарата, особенности течения синдрома спастичности, хронического болевого синдрома на модели левостороннего поперечного пересечения половины (ЛПП) СМ крысы в нижнегрудном отделе.Материалы и методы. Электронейромиография (ЭНМГ): прямая стимуляция СМ (амплитуда М-волны), регистрация Н-рефлекса (соотношение амплитуды Н-волны и М-волны — Н/М, %); верификация спастичности по шкале Ashworth; изучение экстероцептивной чувствительности — методом фон Фрея в модифификации Вейнштейна; функции задних конечностей — по шкале ВВВ.Результаты. Максимальная амплитуда М-волны в исследуемой мышце задней ипсилатеральной (относительно зоны травмы) конечности (ЗИК) на 7-й неделе после ЛПП соответствовала таковой у интактных животных при диссоциации показателя функции (2,5 и 21 балл по шкале ВВВ); до 23-й недели — достоверно уменьшалась. Соотношение Н/М в задних конечностях интактных животных (n=14) составляло в среднем (34,14±3,17)%, в ЗИК (n=10) — (52,44±7,27)% (р

Relevance of the topic. A promising tool for restoring the spinal cord function is the application of tissue neuroengineering. The approbation of its techniques is possible on qualitative spinal cord injury models that makes the transplantation of solid neuroengineered matrices feasible.Objective. To optimize and test a model of lower thoracic rat’s spinal cord hemisection.Materials and methods. 2 groups of experimental animals: 1 — mature animals (3–6 months, n=40) and 2 — young animals (1 month, n=32), the injury — left-side spinal cord hemisection (LHS) at Т11 level; monitoring of hindlimbs’ function (BBB scale), pathomorphological study.Results. LHS provides simultaneous display of a slight, moderate and severe spinal cord injury (the correlates, respectively, are: the function deficit of a contralateral hindlimb [CH] and an ipsilateral hindlimb [IH] among the animals with better and worse recovery indicators). As of the 11th week of the observation a function indicator was 3,2±0,58 points under BBB scale and 5,31±0,79 (p

Isolation of artemisinin from Artemisia annua L. and its quantification by the HPLC-MS method are considered. Different extraction methods were used for the isolation of artemisinin: maceration, ultrasonic and subcritical CO2-extraction. The component content of the CO2- and hexane extracts was studied by the GC-MS method.

Fast monitoring of the cephalosporin antibiotic ceftriaxone in blood and tissue samples was performed in one step by anion-exchange HPLC. The low detection threshold (100 ng/ml in the blood and 50 ng/g in tissues) and a sufficiently high accuracy (4.0–8.0% RSD at a 96–104% recovery) acceptable for usual pharmacokinetic studies were achieved without additional solid-phase extraction steps and ion-pair reagent addition.