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To investigated the role of HIF-1α in myocardial inflammatory injury in rats induced by CME and its possible mechanism. Forty SD rats were separated randomly and equally into four groups, i.e. CME+HIF-1α stabilizer dimethyloxalyl glycine (CME+DMOG) group, CME+HIF-1α inhibitor YC-1 (CME+YC-1) group, CME group, and Sham group. HBFP staining, myocardial enzyme assessment, and cardiac ultrasound were used to measure microinfarct, serum c-troponin I (cTnI) level, and Cardiac function. ELISA and western blot were applied for detecting NLRP3 inflammasome pathway and TLR4/MyD88/NF-κB signaling level.Pro-inflammatory factors of IL-18, IL-1β and TNF-α increased their expression levels after CME, which indicated inflammatory responses in the myocardium. Additionally, in the inflammatory process, NLRP3 inflammasome and TLR4/MyD88/NF-κB signaling were involved. DMOG reverses these effects of CME, whereas YC-1 aggravates these effects. HIF-1α may attenuate myocardial inflammatory injury induced by CME and improve cardiac function, which can perhaps be explained by the fact that TLR4/MyD88/NF-κB signaling pathway activation is inhibited.

Background: Genome-wide association studies (GWAS) have recently shown that Single Nucleotide Polymorphism (SNP) rs17465637 on chromosome 1p41 is associated with atherothrombotic Coronary Artery Disease (CAD). However, whether rs17465637 acts as a protective factor or a risk factor for acute myocardial infarction (AMI) is not well understood in the general population. Objectives: In this article, we aimed to determine whether this locus was related to susceptibility to AMI in a Chinese Han population. Methods: A retrospective experimental study was performed in Guangxi province, People’s Republic of China, on January 1, 2012, to December 31, 2017. We recruited 688 patients who were matched for age, lifestyle, and socioeconomic status from the Chinese Han population and subdivided them into two groups of 344 AMI patients and 344 healthy controls. We used standardized questionnaires to collect information on demographics, socioeconomic status, and lifestyle factors. Genotypes of SNP rs17465637 were determined by the TaqMan assay. Diagnostic criteria and research protocols were based on the guidelines of the European Resuscitation Commission. Statistical analysis was performed by SPSS version 22.0. Results: The percentage of the AA genotype in the AMI group was 22.97%, which was greater than that of the control group (13.08%) (kappa = -0.082, P < 0.001). The AA genotype of SNP rs17465637 had significant differences between different infarct sites (kappa = -0.011, P < 0.05). There were interactions between the CC genotype and BMI ≥ 24 kg/m2 (OR = 4.060, 95% CI = 1.680 - 9.812, P = 0.002) and smoking ≥ 20 cigarettes/d (OR = 2.732, 95% CI = 1.495 - 4.991, P = 0.001). Conclusions: This study revealed that the AA genotype of SNP rs17465637 was positively correlated with the risk of AMI. Subjects with the AA genotype were positively correlated with extensive anterior of AMI. Also, interactions between the CC genotype of SNP rs17465637 and BMI or smoking seem to increase the risk of AMI.

Background: Large-scale population studies showed that the SNP rs1764391 of Connexin37 gene also known as Cx37 gene may play a pivotal role in the occurrence and development of acute myocardial infarction (AMI). Published results, however, are highly controversial. Objective: This study aimed to examine the association between SNP rs1764391 of Cx37 and diseasesusceptibility, several risk factors, and gene-environment interactions of AMI in Guangxi Han Chinese population. Methods: In this study, 344 healthy controls and 344 AMI patients of Han Chinese population were enrolled. The TaqMan assay was implemented to identify genotypes of Cx37 and allele frequencies of SNP rs1764391 in both the AMI and control groups. Results: Significant differences were detected in TT genotype frequencies of SNP rs1764391 between the AMI and control groups (P < 0.05). In the context of gender stratification, the result was also statistically different in women (P < 0.05). Each variable such as age, BMI, diabetes, high blood pressure, smoking and TC was a risk factor and correlated significantly (P < 0.05) with the development of AMI. HDL-C correlated negatively with the risk of AMI (P < 0.001). BMI, smoking or alcohol consumed interacts significantly (P < 0.017) with the presence of the SNP rs1764391 CC genotype. Conclusions: Evidences were presented that Cx37 rs1764391 variation may contribute to the risk for AMI, especially in women and this genetic variant may prove to be a potential biomarker for AMI risk stratification and may prove to be a useful target for therapeutic intervention to further improve prognosis in high-risk patients.

OBJECTIVE The correlation of the BRG1 rs1122608 single nucleotide polymorphism (SNP) with acute myocardial infarction (AMI) has been reported in American and European populations. However, whether rs1122608 acts as a protective factor or a risk factor for AMI is controversial. In this study, we aimed to detect the associations between rs1122608 and the clinical characteristics of AMI as well as susceptibility, gene-environment interactions, and risk factors for AMI in a Chinese Han population. METHODS In this study, 300 AMI patients and 300 healthy controls of Chinese Han ancestry were enrolled. PCR-RFLP was used to genotype rs1122608 SNPs. Genotypic and allelic frequencies of rs1122608 were compared between the AMI and control groups and among four AMI subgroups, which were subdivided by typical symptom, diagnosis time (DT), infarction location andserious complication. RESULTS Significant differences were detected between the AMI patients and the controls in both the genotypic and allelic frequencies of rs1122608 (p