1. Vibriophage D10 contains non-permuted DNA with cohesive ends
- Author
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B. K. Chakrabarti, Dhrubajyoti Chattopadhyay, and Ashutosh Ghosh
- Subjects
Genetics ,biology ,viruses ,Myoviridae ,Genome, Viral ,Nucleic Acid Denaturation ,biology.organism_classification ,medicine.disease_cause ,Virology ,Genome ,Bacteriophage ,chemistry.chemical_compound ,chemistry ,Vibrio cholerae ,DNA, Viral ,medicine ,A-DNA ,DNA ,Genomic organization ,Phage typing - Abstract
Phage D10, a Vibrio cholerae O-1 El Tor group X phage, is one of the five newly isolated phages used in the phage typing scheme developed for V. cholerae O-1 biotype El Tor and belongs to the Myoviridae family. From electron microscopic studies it is shown that phage D10 has a DNA genome of 32 +/- 0.2 kb. This is the first report where it has been shown by the construction of a partial denaturation map that this vibriophage genome is nonpermuted and has cohesive ends. The location of the ends of the DNA in the phage head has also been inferred.
- Published
- 1993