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3 results on '"Anatomy::Cells::Connective Tissue Cells::Osteoblasts [Medical Subject Headings]"'

1. Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts

Author

Manuel Toledano, Concepción Ruiz, Manuel Toledano-Osorio, Raquel Osorio, Víctor J. Costela-Ruiz, Antonio L. Medina-Castillo, Francisco Javier Manzano-Moreno, [Toledano-Osorio,M, Toledano,M, Osorio,R] Colegio Máximo de Cartuja s/n, Faculty of Dentistry, University of Granada, Granada, Spain. [Toledano-Osorio,M] Medicina Clínica y Salud Pública Programme, University of Granada, Granada, Spain. [Manzano-Moreno,FJ] Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, Granada, Spain. [Costela-Ruiz,VJ, Ruiz,C] Instituto Investigación Biosanitaria, ibs.Granada, Granada, Spain. [Medina-Castillo,AL] NanoMyP®, Nanomateriales y Polimeros S.L., Spin-Off Company of the University of Granada, Edificio BIC-Granada, Armilla, Granada, Spain. [Costela-Ruiz,VJ, Ruiz,C] Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain. [Ruiz,C] Institute of Neuroscience, University of Granada, Centro de Investigación Biomédica (CIBM), Parque de Tecnológico de la Salud (PTS), Granada, Spain., and This research was funded by (1) the Ministry of Economy and Competitiveness and European Regional Development Fund [Project MAT2017-85999-P MINECO/AEI/FEDER/UE], (2) University of Granada/Regional Government of Andalusia Research Fund from Spain and European Regional Development Fund (A-BIO-157-UGR-18/FEDER). This research is part of Manuel Toledano-Osorio’s PhD research study.

Subjects
Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Biomimetic Materials::Membranes, Artificial [Medical Subject Headings], Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings], Polymers and Plastics, Osteoblastos, Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Bone Remodeling::Bone Regeneration [Medical Subject Headings], 0302 clinical medicine, Inmunomodulación, Flow cytometry, membrane, Cell proliferation, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Phosphoric Monoester Hydrolases::Alkaline Phosphatase [Medical Subject Headings], Doxycycline, 0303 health sciences, Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biotechnology::Biomimetics [Medical Subject Headings], medicine.diagnostic_test, antigenic phenotype, Chemistry, osteoblasts, Bone regeneration, Cell biology, Membrane, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Separation::Flow Cytometry [Medical Subject Headings], Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Cyclic::Hydrocarbons, Aromatic::Polycyclic Hydrocarbons, Aromatic::Naphthacenes::Tetracyclines::Doxycycline [Medical Subject Headings], Alkaline phosphatase, Fosfatasa alcalina, Periodontal disease, Anatomy::Cells::Connective Tissue Cells::Osteoblasts [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Biological Therapy::Immunomodulation [Medical Subject Headings], medicine.drug, Proliferación celular, Chemicals and Drugs::Inorganic Chemicals::Minerals::Silicon Dioxide [Medical Subject Headings], Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Histocompatibility Antigens Class II::HLA-D Antigens::HLA-DR Antigens [Medical Subject Headings], Article, CD markers, Chemicals and Drugs::Organic Chemicals::Carboxylic Acids::Acids, Acyclic::Acrylates [Medical Subject Headings], Immunomodulation, lcsh:QD241-441, 03 medical and health sciences, lcsh:Organic chemistry, Antígenos CD, medicine, Diseases::Stomatognathic Diseases::Mouth Diseases::Periodontal Diseases [Medical Subject Headings], Regeneración ósea, Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings], Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles [Medical Subject Headings], 030304 developmental biology, CD86, doxycycline, Regeneration (biology), 030206 dentistry, General Chemistry, Membranas, Doxiciclina, Chemicals and Drugs::Inorganic Chemicals::Metals::Metals, Heavy::Zinc [Medical Subject Headings], Enfermedades periodontales, Citometría de flujo, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Analysis of Variance [Medical Subject Headings], CD80
Abstract

This research was funded by (1) the Ministry of Economy and Competitiveness and European Regional Development Fund [Project MAT2017-85999-P MINECO/AEI/FEDER/UE], (2) University of Granada/Regional Government of Andalusia Research Fund from Spain and European Regional Development Fund (A-BIO-157-UGR-18/FEDER). This research is part of Manuel Toledano-Osorio’s PhD research study., Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts' proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease., European Commission MAT2017-85999-P A-BIO-157-UGR-18/FEDER, Ministry of Economy and Competitiveness MAT2017-85999-P, University of Granada/Regional Government of Andalusia Research Fund from Spain A-BIO-157-UGR-18/FEDER

Published
2021

2. SNPs in bone-related miRNAs are associated with the osteoporotic phenotype

Author

De-Ugarte, Laura, Caro-Molina, Enrique, Rodríguez-Sanz, Maria, García-Pérez, Miguel Angel, Olmos, José M., Sosa-Henríquez, Manuel, Pérez-Cano, Ramón, Gómez-Alonso, Carlos, Del Rio, Luis, Mateo-Agudo, Jesús, Blázquez-Cabrera, José Antonio, González-Macías, Jesús, Pino-Montes, Javier del, Muñoz-Torres, Manuel, Diaz-Curiel, Manuel, Malouf Sierra, Jorge, Cano, Antonio, Pérez-Castrillon, José Luis, Nogués Solán, Xavier, Garcia-Giralt, Natalia, Díez Pérez, Adolfo, Universitat Autònoma de Barcelona, Universidad de Cantabria, [De-Ugarte,L, Caro-Molina,E, Rodríguez-Sanz,M, Nogues,X, Garcia-Giralt,N, Diez-Perez,A] IMIM (Hospital del Mar Medical Research Institute), Universitat Autònoma de Barcelona, CIBERFES, RETICEF (ISCIII), Barcelona, Spain. [García-Pérez,MA] Department of Genetics and Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain. [Olmos,JM] Department of Internal Medicine, Hospital Universitario Marqués de Valdecilla-IDIVAL/Hospital de Torrelavega, Universidad de Cantabria. RETICEF, Santander, Spain. [Sosa-Henríquez,M] Unidad Metabólica Ósea, Hospital Universitario Insular, Universidad de Las Palmas de Gran Canaria, Canarias, Spain. [Pérez-Cano,R] Departamento de Medicina (USE), UGC Medicina Interna, Hospital Universitario Virgen Macarena, Seville, Spain. [ Gómez-Alonso,C] Servicio de Metabolismo Óseo y Mineral, Hospital Universitario Central de Asturias, Oviedo, Spain. [Del Rio,L] CETIR Grup Mèdic, RETICEF, Barcelona, Spain. [Mateo-Agudo,J] Servicio COT, Hospital Universitario Miguel Servet, Zaragoza, Spain. [Blázquez-Cabrera,JA] Servicio de Medicina Interna, Complejo Hospitalario Universitario de Albacete, Albacete, Spain. [González-Macías,J] Departamento de Medicina Interna, H. Marqués de Valdecilla, Universidad de Cantabria, IDIVAL, RETICEF, Santander, Spain. [del Pino-Montes,J] Servicio de Reumatología. Hospital Universitario de Salamanca, RETICEF (ISCIII), IBSAL (Biomedical Research Institute of Salamanca), Salamanca, Spain. [Muñoz-Torres,M] UGC Endocrinologia y Nutrición. Hospital Universitario San Cecilio. Granada, RETICEF, Ibs, Granada, Spain. [Diaz-Curiel,M] Unidad de Enfermedades Metabólicas Óseas. Servicio de Medicina Interna. Fundacion Jimenez Diaz, Madrid, Spain. [Malouf,J] Hospital de la Santa Creu I Sant Pau. Institut d’Investigació Biomèdica Sant Pau, Barcelona, Spain. [Cano,A] Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, INCLIVA, Valencia, Spain. [Pérez-Castrillon,JL] Hospital Universitario Río Hortega, Valladolid, Spain., This work was supported by RETICEF (RD12/0043/0022), CIBERFES (CB16/10/00245) and the Grant FIS PI10/01537, PI12/02775, PI12/02582, and PI13/00116 (Carlos III Health Institute, Science and Innovation Ministry), FIS and Grants from the Generalitat de Catalunya (DIUE 2014 SGR 775), '664367/FOCUS' project (EU Health Programme, and 2014–2020) and FEDER funds have supported this study.

Subjects
0301 basic medicine, conformation, Diseases::Wounds and Injuries::Fractures, Bone::Hip Fractures [Medical Subject Headings], Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings], Polimorfismo de nucleótido simple, Gene Expression, bone, Osteoblastos, Densidad ósea, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Cohort Studies, Gene Frequency, single nucleotide polymorphism, Bone Density, Bone cell, Ossos, genetics, Fracturas osteoporóticas, Cells, Cultured, Genetics, Bone mineral, MicroARNs, Multidisciplinary, microRNA, biology, allele, Diseases::Wounds and Injuries::Fractures, Bone::Osteoporotic Fractures [Medical Subject Headings], clinical trial, Middle Aged, cohort analysis, Phenotype, Humanos, Fenotip, medicine.anatomical_structure, Cancellous Bone, osteoblast, Medicine, Diseases::Musculoskeletal Diseases::Bone Diseases [Medical Subject Headings], Phenomena and Processes::Genetic Phenomena::Genotype [Medical Subject Headings], Anatomy::Cells::Connective Tissue Cells::Osteoblasts [Medical Subject Headings], Alelos, Fenotipo, musculoskeletal diseases, medicine.medical_specialty, Genotype, Science, Single-nucleotide polymorphism, Biology, chemistry, Polymorphism, Single Nucleotide, Article, Bone and Bones, Anatomy::Musculoskeletal System::Skeleton::Bone and Bones::Cancellous Bone [Medical Subject Headings], 03 medical and health sciences, Calcification, Physiologic, Internal medicine, medicine, Humans, human, procedures, Allele, Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Alleles [Medical Subject Headings], Alleles, Femoral neck, Genetic association, Aged, cell culture, Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Bone Density [Medical Subject Headings], Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism, Genetic::Polymorphism, Single Nucleotide [Medical Subject Headings], Osteoblasts, Enfermedades óseas, Fracturas de cadera, Computational Biology, Cuello femoral, Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Antisense Elements (Genetics)::RNA, Antisense::MicroRNAs [Medical Subject Headings], MicroRNAs, 030104 developmental biology, Endocrinology, multicenter study, bone mineralization, Nucleic Acid Conformation, Osteoporosis, pathology, Anatomy::Musculoskeletal System::Skeleton::Bone and Bones::Bones of Lower Extremity::Leg Bones::Femur::Femur Neck [Medical Subject Headings], Transcriptome, metabolism, Genotipo, Fractures
Abstract

Biogenesis and function of microRNAs can be influenced by genetic variants in the pri-miRNA sequences leading to phenotypic variability. This study aims to identify single nucleotide polymorphisms (SNPs) affecting the expression levels of bone-related mature microRNAs and thus, triggering an osteoporotic phenotype. An association analysis of SNPs located in pri-miRNA sequences with bone mineral density (BMD) was performed in the OSTEOMED2 cohort (n = 2183). Functional studies were performed for assessing the role of BMD-associated miRNAs in bone cells. Two SNPs, rs6430498 in the miR-3679 and rs12512664 in the miR-4274, were significantly associated with femoral neck BMD. Further, we measured these BMD-associated microRNAs in trabecular bone from osteoporotic hip fractures comparing to non-osteoporotic bone by qPCR. Both microRNAs were found overexpressed in fractured bone. Increased matrix mineralization was observed after miR-3679-3p inhibition in human osteoblastic cells. Finally, genotypes of rs6430498 and rs12512664 were correlated with expression levels of miR-3679 and miR-4274, respectively, in osteoblasts. In both cases, the allele that generated higher microRNA expression levels was associated with lower BMD values. In conclusion, two osteoblast-expressed microRNAs, miR-3679 and miR-4274, were associated with BMD; their overexpression could contribute to the osteoporotic phenotype. These findings open new areas for the study of bone disorders.

Published
2017

3. Influence of high glucose and advanced glycation end-products (ages) levels in human osteoblast-like cells gene expression

Author

M. José Miranda, Ramón Pérez-Cano, M. José Montoya, C. Miranda, M. Angeles Vázquez, Mercè Giner, [Miranda, Cristina] Virgen Macarena Univ Hosp, Dept Internal Med, Bone Metab Unit, Seville 41009, Spain, [Giner, Merce] Virgen Macarena Univ Hosp, Dept Internal Med, Bone Metab Unit, Seville 41009, Spain, [Jose Miranda, M.] Virgen Macarena Univ Hosp, Dept Internal Med, Bone Metab Unit, Seville 41009, Spain, [Perez-Cano, Ramon] Virgen Macarena Univ Hosp, Dept Internal Med, Bone Metab Unit, Seville 41009, Spain, [Giner, Merce] Univ Seville, Dept Med, E-41009 Seville, Spain, [Jose Montoya, M.] Univ Seville, Dept Med, E-41009 Seville, Spain, [Angeles Vazquez, M.] Univ Seville, Dept Med, E-41009 Seville, Spain, [Perez-Cano, Ramon] Univ Seville, Dept Med, E-41009 Seville, Spain, Research Project of Excellence, Junta de Andalucia (Spain), RETICEF, [Miranda,C, Miranda,MJ, Pérez-Cano,R] Bone Metabolism Unit, Internal Medicine Department, Virgen Macarena University Hospital, Seville, Spain. [Giner,M, Montoya,MJ, Vázquez,MA, Pérez-Cano,R] Medicine Department, University of Seville, Seville, Spain., and Funding was supported by the Research Project of Excellence, Junta de Andalucía (Spain) (CTS-5360/09) and by ISCIII 2013-RED 12–0043 (RETICEF).

Subjects
0301 basic medicine, Glycation End Products, Advanced, Male, Diseases::Wounds and Injuries::Fractures, Bone::Hip Fractures [Medical Subject Headings], Type-2 diabetes-mellitus, Osteoporosis, Chemicals and Drugs::Carbohydrates::Monosaccharides::Hexoses::Glucose [Medical Subject Headings], Gene Expression, Core Binding Factor Alpha 1 Subunit, Apoptosis, Osteoarthritis, Hip, Osteoblastos, Bone remodeling, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], In-vitro, 0302 clinical medicine, Glycation, Osteogenic differentiation, Medicine, Orthopedics and Sports Medicine, Fracturas osteoporóticas, Aged, 80 and over, biology, Receptor de productos finales de la glicosilación avanzada, Diseases::Musculoskeletal Diseases::Joint Diseases::Arthritis::Osteoarthritis::Osteoarthritis, Hip [Medical Subject Headings], Diseases::Wounds and Injuries::Fractures, Bone::Osteoporotic Fractures [Medical Subject Headings], Rankl, Osteoblast, RUNX2, medicine.anatomical_structure, Messenger-rna, RANKL, Sp7 Transcription Factor, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Advanced Glycosylation End Product-Specific Receptor [Medical Subject Headings], Diseases::Nutritional and Metabolic Diseases::Metabolic Diseases::Glucose Metabolism Disorders::Diabetes Mellitus::Diabetes Mellitus, Type 2 [Medical Subject Headings], Female, Bone Remodeling, Anatomy::Cells::Connective Tissue Cells::Osteoblasts [Medical Subject Headings], Research Article, Receptor, musculoskeletal diseases, medicine.medical_specialty, Fracture risk, Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Carbohydrate Metabolism::Glycosylation [Medical Subject Headings], Primary Cell Culture, 030209 endocrinology & metabolism, Bone and Bones, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Tumor Necrosis Factors::RANK Ligand [Medical Subject Headings], 03 medical and health sciences, Rheumatology, Remodelación ósea, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Vital Statistics::Morbidity::Incidence [Medical Subject Headings], Internal medicine, Diabetes mellitus, Humans, Bone, Glicosilación, Aged, Osteoblasts, business.industry, Hip Fractures, RANK Ligand, Fracturas de cadera, Osteoprotegerin, Type 2 Diabetes Mellitus, Ligando RANK, medicine.disease, Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Bone Remodeling [Medical Subject Headings], 030104 developmental biology, Endocrinology, Glucose, Diabetes Mellitus, Type 2, Diabetes mellitus tipo II, Glucosa, biology.protein, business, Biomarkers, Osteoporotic Fractures, Osteoartritis de la cadera
Abstract

Background Type 2 diabetes mellitus (T2DM) is associated with an increased risk of osteoporotic fracture. Several factors have been identified as being potentially responsible for this risk, such as alterations in bone remodelling that may have been induced by changes in circulating glucose or/and by the presence of non-oxidative end products of glycosylation (AGEs). The aim of this study is to assess whether such variations generate a change in the gene expression related to the differentiation and osteoblast activity (OPG, RANKL, RUNX2, OSTERIX, and AGE receptor) in primary cultures of human osteoblast-like cells (hOB). Methods We recruited 32 patients; 10 patients had osteoporotic hip fractures (OP group), 12 patients had osteoporotic hip fractures with T2DM (T2DM group), and 10 patients had hip osteoarthritis (OA group) with no osteoporotic fractures and no T2DM. The gene expression was analyzed in hOB cultures treated with physiological glucose concentration (4.5 mM) as control, high glucose (25 mM), and high glucose plus AGEs (2 μg/ml) for 24 h. Results The hOB cultures from patients with hip fractures presented slower proliferation. Additionally, the hOB cultures from the T2DM group were the most negatively affected with respect to RUNX2 and OSX gene expression when treated solely with high glucose or with high glucose plus AGEs. Moreover, high levels of glucose induced a major decrease in the RANKL/OPG ratio when comparing the OP and the T2DM groups to the OA group. Conclusions Our data indicates an altered bone remodelling rate in the T2DM group, which may, at least partially, explain the reduced bone strength and increased incidence of non-traumatic fractures in diabetic patients.

Published
2016

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