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Rationale: 3-Monochloropropane-1,2-diol (3-MCPD) is a well-known contaminant formed in food thermal processing, which could be found in a variety of foodstuffs. Due to its potential carcinogenicity, it was essential to quickly develop a rapid and high-throughput analytical method to monitor 3-MCPD in foodstuffs, which is described in this study., Methods: 3-MCPD was extracted from foodstuffs and then was derivatized with a boronic acid-modified C 60 (B-C 60 ) through the boronic acid-diol reaction. Microwave heating was used to accelerate the derivatization reaction. Mass spectrometry (MS) analysis was conducted using matrix-assisted laser desorption/ionization-MS (MALDI-MS). The application of the method was validated using various smoked food samples., Results: The chemical derivatization of 3-MCPD with B-C 60 enabled the addition of a C 60 -tag to 3-MCPD. High-throughput analysis of the sample within 0.5 h was realized. A good linear range from 0.02 to 1.5 μg mL -1 for 3-MCPD was obtained, with a detection limit of 0.005 μg mL -1 . The recoveries in spiked foodstuffs ranged from 85.4% to 115.1% with relative standard deviations of 2.0%-14.2%. This method was successfully applied to detect 3-MCPD in smoked foodstuffs., Conclusions: A quantitative method was developed for the detection of 3-MCPD in foodstuffs using B-C 60 derivatization combined with MALDI-MS strategy. This proposed method may serve as a potential platform for the rapid and high-throughput analysis of 3-MCPD in foodstuffs for the purpose of food safety control., (© 2021 John Wiley & Sons Ltd.)

The meat industry requires prompt and effective control measures to guarantee the quality and safety of its products and to avert the incidence of foodborne illnesses and disease outbreaks. Although standard microbiological methods and conventional analytical techniques are employed to monitor the quality and safety, these procedures are tedious and time-consuming, require skilled technicians, and sophisticated instruments. Therefore, there is an urgent need to develop simple, fast, and user-friendly hand-held devices for real-time monitoring of the quality of meat and meat products in the supply chain. Biosensors and chemical indicators, due to their high sensitivity, specificity, reproducibility, and stability, are emerging as promising tools and have the potential for monitoring and controlling the quality (freshness and sensory traits such as tenderness) and safety (metabolites, contaminants, pathogens, drug residues, etc.) of muscle foods. In this review, the application of biosensors in the meat industry and their emerging role in the quantification of key meat quality components are discussed. Furthermore, the role of different biosensors to identify and detect contaminants, adulterants, pathogens, antibiotics, and drug residues in meat and meat products is also summarized. [ABSTRACT FROM AUTHOR]

Consumers are increasingly demanding foods that are more ethical, sustainable and nutritious to improve the health of themselves and the planet. The food industry is currently undergoing a revolution, as both small and large companies pivot toward the creation of a new generation of plant-based products to meet this consumer demand. In particular, there is an emphasis on the production of plant-based foods that mimic those that omnivores are familiar with, such as meat, fish, egg, milk, and their products. The main challenge in this area is to simulate the desirable appearance, texture, flavor, mouthfeel, and functionality of these products using ingredients that are isolated entirely from botanical sources, such as proteins, carbohydrates, and lipids. The molecular, chemical, and physical properties of plant-derived ingredients are usually very different from those of animal-derived ones. It is therefore critical to understand the fundamental properties of plant-derived ingredients and how they can be assembled into structures resembling those found in animal products. This review article provides an overview of the current status of the scientific understanding of plant-based foods and highlights areas where further research is required. In particular, it focuses on the chemical, physical, and functional properties of plant-derived ingredients; the processing operations that can be used to convert these ingredients into food products; and, the science behind the formulation of vegan meat, fish, eggs, and milk alternatives., (© 2021 Institute of Food Technologists®.)

• Paper- based LAMP-LF Assay was developed for onsite detection of pork derivatives. • Lyophilized paper LAMP buttons were designed as a ready to use master-mix. • Sampling to end –point detection was established in less than 3 hours. • Storage stability of LAMP reagents carrier buttons were 4 months at 4 °C. Identification of meat product adulteration is important to help fair-trade and to enable consumers to make informed choices. Detection of meat adulteration requires reliable analytical methods. Here, we report development of a loop-mediated isothermal amplification (LAMP) method for the detection of tissues of pig origin. We developed a kit containing lyophilized paper-buttons impregnated with the LAMP master-mix together with Lateral Flow Assay (LFA) paper strips. This paper-based LAMP-LFA kit is a ready to use device for remote settings and useful for the quick and efficient detection of tissue of pig origin. LAMP primers targeting mitochondrial COI gene and corresponding probes specific to LAMP amplicon were designed. LAMP reaction component, optimization details regarding thermal amplification protocol, hybridization conditions for probes, design of LFA paper-strips, and detection methods are provided. The paper-based LAMP-LFA platform allowed analysis of the amplified products using HNB dye-based quick visual method and gel electrophoresis-based validation to avoid personal bias. Assay limit-of-detection was 10 fg of DNA template in the reaction. Assay was validated on samples from different pigs, coded samples, binary meat admixture, diversely processed meat to mimic field situation, and supernatant from Phire Animal Tissue Direct PCR kit. The paper-based LAMP-LFA results were comparable with the pre-standardized species-specific polymerase chain reaction, a time consuming and expensive process. Lyophilized paper-based LAMP buttons in the form of a ready-to-use kit were developed to store reaction mixture. LAMP buttons were stable for up to four months when stored at 4 °C. Developed assay is capable of a rapid (3 hrs) identification of tissue of pig origin in tested samples, cost-effective, user-friendly, and requires minimal training making it a method of choice in remote point-of-care sites. [ABSTRACT FROM AUTHOR]

This study was conducted to isolate and identify extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales in conventional and organic chicken meats, which were sold in Turkey. A total of 200 raw chicken meat sample (100 conventional and 100 organic) were used as material. Classic culture technique based on chromogenic method was used for the isolation of bacteria, and the identification was performed with VITEK MS. Phenotypic ESBL production was detected by combined disc diffusion method. Gene regions responsible for ESBL production were determined by PCR. MIC values of isolates were detected by VITEK 2. Phenotypic ESBL-producing Enterobacterales were detected in 46% of conventional chicken meats and in 22% of organic chicken meats. Of the 115 isolates obtained, 97 (84%) were Escherichia coli, 12 (10%) were Klebsiella pneumoniae, four (3·48%) were Serratia fonticola, one (0·87%) was Rahnella aquatilis, and one (0·87%) was Serratia liquefaciens. PCR analysis revealed that 109 of 115 isolates (94·78%) contained at least one of the bla CTX-M , bla TEM , and bla SHV genes. Of the 115 ESBL-producing isolates, 103 (89·57%) were found resistant to at least one antibiotic except for the β-lactam group. The contamination level of ESBL-producing Enterobacterales was higher in conventional chicken meats (P < 0·001)., (© 2021 The Society for Applied Microbiology.)

Beef is considered an excellent source of high-quality protein and micronutrients. The high saturated fatty acid (SFA) composition of beef has been associated with cardiovascular diseases; however, this is a controversial issue because at present, no evidence has scientifically proven such an association. Wagyu cattle have been used as an option to generate high-quality fatty acids (FAs) in beef and have been crossed with local beef cattle (Wagyu-Cross). In Mexico, Wagyu-Cross is marketed assuming the same quality as purebred Wagyu meat without scientific support. This study aimed to determine whether the differences in the FA composition of Wagyu-Cross and commercial beef affected lipid metabolism in frequent consumers of beef. The study is a randomized, controlled, double-blinded phase IV clinical trial. Thirty-four volunteers completed treatments in which 120 g ground beef was consumed 3 days per week for 2 weeks. Volunteers were randomized to the "A" (commercial meat) or "B" (Wagyu-Cross meat) groups. The beef fat content was 87% higher, the SFA content was 2.3% lower, and the monounsaturated fatty acid (MUFA) C18:1 n-9 concentrations were higher in Wagyu-Cross beef than in commercial beef. The B group exhibited a significantly larger change from baseline in the serum lipid profile in total cholesterol (TC) (-4.7% versus +6.9%), low-density lipoprotein (LDL) (-4.1% versus +10.0%), nonhigh-density lipoprotein (non-HDL) (-6.1% versus +4.9%), and the ratio of three atherogenic indexes-TC/HDL (-3.5% versus +6.4%), LDL/HDL (-2.8% versus +14.6%), and non-HDL/HDL (-4.7% versus +9.3%)-than the A group. This result confirms that meat FA composition is a key point for redirecting cattle breeding strategies and promotes beef as a healthful protein source. PRACTICAL APPLICATION: Here, we found new evidence based on a clinical trial that beef with a favorable fatty acid composition (i.e., monounsaturated fatty acid and polyunsaturated fatty acid content) is a key factor in improving parameters associated with consumer health. This information will support the design of cattle breeding strategies as an option to promote beef consumption as a healthier protein source and create opportunities for the development of the functional food industry., (© 2021 Institute of Food Technologists®.)

Ankyrin 1 (ANK1) gene has been demonstrated to be a functional candidate gene for meat quality that helps to constitute and maintain the structure of the cell skeleton. In this study, three contiguous ANK1 regions from yak were analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP). As a result, nine single-nucleotide polymorphisms (SNPs) were identified, four of them in the coding region and three (c.179 C/A, c.250 G/C, and c.313 C/T) putatively resulting in amino acid changes (p. Ala 60 Glu, p. Asp 84 His, and p. Pro 105 Ser). Some SNPs in promoter region were located within or nearby the putative transcription factor binding sites, such as Sp1 and GATA, which might have an impact on the expression of the yak ANK1 gene. The presence of C 1 -D 3 and C 1 -A 3 were associated with an increased hot carcass weight (p = 0.0045) and a decreased drip loss rate (p = 0.0046). The presence of B 1 -B 3 , C 1 -A 3 and C 1 -D 3 had decreased Warner-Bratzler shear force (p = 0.0066, p = 0.0343 and p = 0.0004). The presence of one and two copies of B 1 -B 3 and C 1 -A 3 had decreased Warner-Bratzler shear force (p = 0.0005 and p = 0.0443), and C 1 -A 3 had also decreased drip loss rate (p = 0.0164). These findings indicated that genetic variations of the ANK1 gene would be a preferable biomarker for the improvement of yak meat quality.

Increasing awareness of inefficient meat production and its future impact on global food security has led the food industry to look for a sustainable approach. Meat products have superior sensorial perception, because of their molecular composition and fibrous structure. Current understanding in the science of food structuring has enabled the utilization of alternative or nonmeat protein ingredients to create novel structured matrices that could resemble the textural functionality of real meat. The physicochemical and structural changes that occur in concentrated protein systems during thermomechanical processing lead to the creation of a fibrous or layered meat-like texture. Phase transitions in concentrated protein systems during protein-protein, protein-polysaccharide, protein-lipid, and protein-water interactions significantly influence the texture and the overall sensory quality of meat analogs. This review summarizes the roles of raw materials (moisture, protein type and concentration, lipids, polysaccharides, and air) and processing parameters (temperature, pH, and shear) in modulating the behavior of the protein phase during the restructuring process (structure-function-process relationship). The big challenge for the food industry is to manufacture concept-based (such as beef-like, chicken-like, etc.) meat analogs with controlled structural attributes. This information will be useful in developing superior meat analogs that fulfill consumer expectations when replacing meat in their diet., (© 2021 Institute of Food Technologists®.)

Innovation in cultivated meat development has been rapidly accelerating in recent years because it holds the potential to help attenuate issues facing production of dietary protein for a growing world population. There are technical obstacles still hindering large-scale commercialization of cultivated meat, of which many are related to the media that are used to culture the muscle, fat, and connective tissue cells. While animal cell culture media has been used and refined for roughly a century, it has not been specifically designed with the requirements of cultivated meat in mind. Perhaps the most common industrial use of animal cell culture is currently the production of therapeutic monoclonal antibodies, which sell for orders of magnitude more than meat. Successful production of cultivated meat requires media that is food grade with minimal cost, can regulate large-scale cell proliferation and differentiation, has acceptable sensory qualities, and is animal ingredient-free. Much insight into strategies for achieving media formulations with these qualities can be obtained from knowledge of conventional culture media applications and from the metabolic pathways involved in myogenesis and protein synthesis. In addition, application of principles used to optimize media for large-scale microbial fermentation processes producing lower value commodity chemicals and food ingredients can also be instructive. As such, the present review shall provide an overview of the current understanding of cell culture media as it relates to cultivated meat., (© 2020 Institute of Food Technologists®.)

To improve the quality of meat products is a constant focus for both the meat industry and scientists. As major components in meat protein, the gelation properties of myofibrillar proteins (MPs) predominantly determine the sensory quality and product yield of the final product. Naturally or artificially occurring covalent modifications are known to largely affect MP functionality by changing the protein structure and forming aggregates, leading to both favorable and unfavorable outcomes. The review aims to summarize the mechanisms associated with several covalent modifications and the recent developments in enhancing MP gelation properties. Various extrinsic and intrinsic parameters controlling oxidation, phenolic-protein interactions, enzyme catalysis, glycation, and isoelectric solubilization/precipitation, and their effects on the characteristics of heat-induced MP gels are discussed. This article provides an improved understanding of the covalent modifications that occur mainly in the MP system and how they can be utilized to promote its gelation properties. Covalent modifications exhibited dose-dependent and dual-role manners for MP gelation properties. Mild oxidation, enzyme catalysis, and isoelectric solubilization/precipitation treatment would be beneficial to form more aligned and cross-linked three-dimensional networks for MP gels because of moderate protein aggregation. However, an excessive aggregate impedes the MP gelation behavior, leading to reduced gelation quality. Glycation effectively increased hydrophilicity of MPs and phenolic conjugation provides MPs with novel bioactivity. A proper utilization of such a process or even a rational combination of them allowed us to enhance the gelation properties of MP with assorted appreciated functionalities and further improve the quality of meat products., (© 2020 Institute of Food Technologists®.)

Purpose: In the last few years there has been considerable talk of the 'Stone Age diet' as an attractive alternative to the 'modern' diet. The underlying idea is that recent distortions of diet are likely to be harmful and that, if we can recover a primeval diet, we will live in closer harmony with our digestive systems and their evolutionary biochemistry. This paper aims to evaluate that set of ideas rigorously in relation to the extensive and sometimes detailed records of the past. Design: Comparative and direct palaeoanthropology and archaeology. Methodology: To review evidence of the comparative picture established by the nearest living relations of hominids (extant apes), and of the deep view given by the fossil records and archaeological evidence. Results: The broad outline of the evolutionary record is becoming increasingly clear: human ancestors diverged from the last common ape ancestor about 8-10 million years ago. Since then major stages of dietary evolution can be traced, involving the incorporation of roots/tubers and additional meat into a previously largely fruit-based diet. Within the last 2 million years, early humans colonized highly diverse environments, including arctic regions where there was no choice but to eat meat. Conclusions: There was no one Stone Age diet. Overall, diets of the past varied greatly. The adoption of agriculture within the last few thousand years led to great changes in diet, especially concentration on single cereals and milk. [ABSTRACT FROM AUTHOR]

The paper aimed to analyze the dynamics of livestock and milk and meat production in the top five EU countries growing sheep and goats: Spain, Romania, Greece, France and Italy. The empirical data from Eurostat and Faostat for the period 2012-2021 were processed using usual methods and procedures like fixed basis and structural indices, trend regression equation, determination coefficient and points method. The comparison between these five countries pointed out the decline of sheep number in four countries, except Romania and the decrease in goats number in Greece and Spain, but an increase in Romania, France and Italy. Ewe milk production increased in three countries, except Spain where it remained relatively constant and Romania where it declined. Goat milk production raised in three countries, except Greece and Romania. Sheep meat output increased in France and Spain, but in the other countries went down, while goat meat output increased only in Spain, the other four states recording a decline. In the year 2021, these five countries all together counted 46.1 million sheep, representing 76.% of the EU sheep livestock, and also they had 9.4 million goats, meaning 82.1% of the EU goats livestock. In 2020, all these countries produced 2.73 million tonnes sheep milk and 1.87 million tonnes goat milk, accounting for 1.7% and respectively 1.16% in the EU milk output. These counties also contributed by 85% to the EU sheep and goat meat production. This reflects the importance of small ruminants in the EU animal livestock and production. These species have to help the EU for greening agriculture, as they could valorize natural resources from the mountains and less favored areas and also could be grown in organic farming system, assuring jobs and income to the local population, food security and at the same time contributing to the biodiversity preservation and production of renewable energy. [ABSTRACT FROM AUTHOR]

This study aimed to compare growth and slaughter performance of layer-type slow-growing Rhode Island Red male chickens grown with and without access to an area with vegetation in the tropics. Raised until 115 days of age, a total of 156, 1-day-old birds were randomly allocated to two treatments: total confinement (TC) and partial confinement (PC), with 6 replicates of 13 birds each. Birds grown under TC conditions reached a live weight of 2016.68 g while those raised with access to outdoors attained 1981.07 g, without statistical differences. Feed intake and daily feed intake were higher (P < 0.05) in birds farmed with TC management, during the last 17 days of the trial. Treatments did not significantly (P > 0.05) affect bird's other productive parameters and carcass traits. Results from this study suggested that slow-growing Rhode Island Red males may be raised with access to outdoors without any adverse effect on productive performance and carcass quality in the Mexican Tropic. Based on the results of this study, a rearing period of 84 days of age is recommended for Rhode Island Red male chickens raised in both TC and PC production systems.

Present study was aimed to evaluate the hatching traits and subsequent performance of broilers strains under the intermittent and continuous light regime during incubation. In total, 2250 eggs from Hubbard classic, Cobb-500, and Ross-308 strains (750 eggs from each of same age breeders) were incubated under three different light durations. First treatment was the incubation totally under darkness where no light was able to penetrate in the assigned section of machine. In the second treatment, eggs were incubated at 12 h of lightness and 12 h of darkness. In the third treatment, the eggs received lightning of 24 h. Data were collected for hatching traits and hatch window, growth performance, welfare aspects, and meat quality. A two-way factorial analysis was performed using SAS software applying Duncan's multiple range test. The results showed that hatching traits were improved when Hubbard breeder eggs were provided with light period of 12 h. However, gait score was non-significantly different among the treatment. The meat quality was better in Hubbard broilers obtained after 12 h of intermittent light during incubation. Blood biochemistry was also improved in Hubbard broilers of 12 h of light duration. It was concluded that 12 h of light period during incubation is beneficial for getting better hatchability and subsequent performance of Hubbard broilers.

Recently, there has been growing interest in implementing innovative nanoscience-based technologies to improve the health, safety, and quality of food products. A major thrust in this area has been to use nanoemulsions because they can easily be formulated with existing food ingredients and technologies. In particular, oil-in-water nanoemulsions, which consist of small oil droplets (<200 nm) dispersed in water, are being utilized as delivery systems for various hydrophobic substances in foods, including nutrients, nutraceuticals, antioxidants, antimicrobials, colors, and flavors. In this article, we focus on the application of nanoemulsion-based delivery systems for improving the quality, safety, nutritional profile, and sensory attributes of muscle foods, such as meat and fish. The article also critically reviews the formulation and fabrication of food-grade nanoemulsions, their potential benefits and limitations in muscle food systems., (© 2020 Institute of Food Technologists®.)

To evaluate how eucalyptus leaf polyphenol extract (EPE) affects chicken meat color and taste, we added different levels of EPE (0%, 0.06%, 0.09%, and 0.12%) to chicken feed. The redness (a * value) and the myoglobin content of breast muscle in EPE group were remarkably higher. Furthermore, the guanosine monophosphate, histidine, and glycine muscle contents were also enhanced. Transcriptome analysis showed that 10 candidate genes related to meat quality were affected by EPE treatment. The identified genes, with functions critical to chicken meat color and taste, will help to determine the molecular mechanisms of EPE., (© 2020 Institute of Food Technologists®.)

γ-Aminobutyric acid (GABA) is a natural nonprotein amino acid distributed in animals, plants and microbes. GABA is an inhibiting neurotransmitter which takes great effect in mammalian central nervous system. We carried out the research to study the influence of GABA on blood hormone concentrations, antioxidant status and meat quality in fattening pigs after transportation. The 72 pigs with a starting weight of approximately 32.67 ± 0.62 kg were randomly allocated to 2 groups based on dietary treatments, containing 6 replicates with 6 pigs in each. The pigs were fed dietary supplementation of GABA (0 or 30 mg/kg of diets) for 74 days. Twelve pigs were randomly selected from each group and assigned to the either 1 hr of transport (T group) or no transport (N group), resulting in two-factor factorial design. Compared to the control, GABA supplementation increased average daily gain (ADG) (p < .01) and decreased feed-gain ratio (F/G) (p < .05). The pH 45 min was lower and drip loss was greater in the longissimus muscles (LM) of post-slaughter of transported pigs (p < .05). The pH 45 min of 0/T group (group with 0 mg/kg GABA and transport) was significantly lower than the pH 45 min of the 30/T group (diet × transport; p < .05). GABA supplementation significantly increased serum glutathione peroxidase (GSH-Px) concentration (p < .05) before transportation. Following transport, pigs fed GABA had decreased concentrations of serum malonaldehyde (MDA), adrenal cortical hormone and cortisol (p < .05). The results indicate that feeding GABA significantly increased the growth performance of growing-finishing pigs. The transportation model negatively impacted meat quality, antioxidant indexes and hormone parameters, but dietary supplementation of GABA could suppress the rise of drip loss of LM, ACTH and COR and suppress the drop of pH 45 min of LM after transportation stress in growing-finishing pigs. Feeding GABA alleviated transportation stress in pigs., (© 2019 Blackwell Verlag GmbH.)

This study was done to evaluate the effects of different dietary digestible lysine (dig Lys) levels on growth performance, blood metabolites, carcass and breast yield, and breast meat quality of broilers 23-38 days of age. Three hundred 23-day-old Cobb-500 male broiler chickens were allocated to a completely randomized design with five treatments (finisher diet containing 0.88%, 0.94%, 1.00%, 1.06% and 1.12% dig Lys) and six replicates of 10 birds each. Feed intake (FI) was not affected by different dietary dig Lys levels. Weight gain (WG) linearly increased and feed conversion ratio (FCR) linearly decreased with an increasing dietary dig Lys levels. With increasing dietary dig Lys levels, carcass and breast yield and breast meat hue angle (h * ) linearly increased, but abdominal fat decreased quadratically, whereas breast meat lightness (L * ) and ether extract composition linearly decreased. Dietary dig Lys levels did not show any significant effect on serum metabolites, breast meat cooking loss and water holding capacity. Based on the linear broken-line regression models, the weight gain, feed conversion ratio and breast yield were optimized when dietary dig Lys levels were 0.95%, 1.01% and 1.02% respectively. It is concluded that Lys requirements vary according to what productive parameter is taken for optimization. A minimum of 1.02% dig Lys concentration in the finisher diet is suggested to optimize breast yield, feed efficiency and performance in broiler chickens., (© 2019 Blackwell Verlag GmbH.)

A study was conducted to determine the effects of flaxseed supplementation on performance, carcass traits, and hindleg fatty acid composition of guinea pigs. Sixty male and female weaned guinea pigs (1 month old, five animals/cage) were blocked by sex and bodyweight and randomly fed 0 (control) or 100 g/kg flaxseed concentrate diets (15 g/animal) plus ad libitum fresh alfalfa for 30 days. Results showed that flaxseed supplementation had no influence on animal performance. However, final body weight (P = 0.035), total feed intake (P = 0.019), and body weight gain (P < 0.001) were higher in male than female guinea pigs. Similar results were also observed for carcass composition (i.e., hot, chilled, and reference carcass weights). Inclusion of flaxseed reduced saturated (P < 0.001), mono-unsaturated (P = 0.004), and increased (P < 0.001) polyunsaturated (PUFA) fatty acid concentrations in hindlegs. Concentrations of linolenic acid and n-3 PUFA increased (P < 0.001) by 49.7 and 37.1%, respectively as a result of flaxseed inclusion. It was concluded that feeding flaxseed to guinea pigs at 100 g/kg of the concentrate diets improves meat PUFA concentrations with no adverse effects on performance or carcass composition.

The study was conducted to evaluate breast meat quality attributes of Marshal and FUNAAB Indigenous (Alpha C13 and Alpha C14) Broiler (FIB) chickens. Birds of average live weights (1.8 to 2.0 kg) were selected from flock fed same concentrate diet and managed under deep litter system. Birds were slaughtered, scalded in hot water at 65 °C, de-feathered, and frozen-stored. Proximate, mineral, cholesterol contents, pH, color, and fatty acid profile of the chickens' meat were determined. Data were subjected to ANOVA (p < 0.05). Moisture (73.87%) and fat (2.82%) contents of Marshal were higher than FIB. Protein (23.65%) and ash (1.56%) were highest (p < 0.05) for Alpha C14. pH ranged between 5.94 and 6.58. Lightness (L*) and redness (a*) values were 69.37-75.52 and 8.77-7.96. Alpha C13 had significantly higher L* and a* than Marshal. Potassium was most abundant mineral observed across breeds. Saturated (SFA, 38.03-57.10%), monounsaturated (MUFA, 29.78-41.55%), and polyunsaturated (PUFA, 10.91-20.29%) fatty acid compositions differed significantly among breeds. Cholesterol contents of Alpha C14 (4.23 mg/kg) was significantly lower, while Marshal (5.67 mg/kg) chicken showed significantly higher value. Conclusively, FIB chickens are valuable nutritional meat sources with desirable chemical composition and can constitute alternative chicken meats with human health benefit.

The objective of this study was to evaluate carcass characteristics, meat quality, and organ and viscera weight of forty male sheep, not castrated, initially weighed 26.5 ± 1.85 kg, and confined for 100 days (44 days for the adjustment period and 56 days of the experimental period). The treatments were four levels of replacement of Bermuda grass hay with alfalfa hay (0, 200, 400, and 600 g/kg, based on dry matter). The design was randomized blocks (one lighter group and other heavier group) with 10 animals in each treatment. There were no effects on weight gain or carcass parameters. However, an increase was observed on dry matter intake (g/day), crude protein intake (g/day), empty body weight (kg), and subcutaneous fat thickness (mm). There was also an increase in total organ weights in kg and g/kg. The tissue composition of the leg had a quadratic effect on the bone tissue weight (g) and linear effect on adipose tissue relative (g/kg) and muscle-fat relations. There was also a quadratic effect on bone tissues (g/kg) and muscle-bone relations. Likewise, there was a quadratic effect on the qualitative characteristics of meat (measure of luminosity), with the replacement of Bermuda grass hay with alfalfa hay in sheep feed. The replacement of Bermuda grass hay with alfalfa did not change carcass characteristics and meat quality.