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Dynamic X-ray elastography using propagating shear waves is a novel technique to visualize the spatial distribution of elasticity in soft materials. We demonstrate that it can be applied to a gel sample with a fine structure. We prepared a viscoelastic gel sample with a two-dimensional lattice structure filled with ZrO2 particles, and applied the technique to map storage and loss moduli. The obtained storage modulus map successfully distinguished the harder lattice structure from the soft gel matrix with a spatial resolution much higher than those of other conventional elastography techniques using magnetic resonance and ultrasound.

In this study, the longitudinal wave speed (LWS) and shear wave speed (SWS) of the homogeneous agar-glycerol phantoms were ultrasonically measured, and the relationship between the compositions of phantoms and these two speeds was investigated. From the viewpoint of the measurement sensitivity of LWS and SWS, the LWS had the sensitivity to discriminate the differences in the glycerol weight concentration, while the SWS did not exhibit such sensitivity. These results indicate that the LWS can discriminate the composition of tissues different from that by the SWS in clinical diagnosis.

The present study aimed to develop vibration techniques for magnetic resonance (MR) elastography (MRE) of the psoas major muscle (PM). Seven healthy volunteers were included. MRE was performed with motion-encoding gradient (MEG)-less multi-echo MRE sequence, which allows clinicians to perform MRE using conventional MR imaging. In order to transmit mechanical vibration of the pneumatic type to the PM, a long narrow vibration pad was designed using a 3D printer, and the optimum vibration techniques were verified. The vibration pad was placed under the lower back, with the volunteers in the supine position. The results indicated that the PM vibrated well through the transmitted vibration from the lumbar spine, which suggests that the placement of a narrow vibration pad under the supine body, along the lumbar spine, allows the vibration of the PM. The shear modulus of the PM (n = 7) was 1.23 ± 0.09 kPa (mean ± SEM) on the right side and 1.22 ± 0.15 kPa on the left side, with no significant difference (t-test, P > 0.05). Increased stiffness of the muscle due to continuous local contraction may be an important cause of non-specific low back pain (LBP). The present vibration techniques for MRE of the PM provide a quantitative diagnostic tool for changes in muscle stiffness associated with non-specific LBP.

Palpation is a standard clinical tool to diagnose abnormal stiffness changes in soft tissues. However, it is difficult to palpate the supraspinatus muscle because it locates under the trapezius muscle. The magnetic resonance elastography (MRE) uses harmonic mechanical excitation to quantitatively measure the stiffness (shear modulus) of both the superficial and deep tissues. The purpose of this study was to build a vibration system for applying the MRE to the supraspinatus muscle. In this study, a power amplifier and a pneumatic pressure generator were used to supply vibrations to a vibration pad. Six healthy volunteers underwent MRE. We investigated the effects of position (the head of the humerus and the trapezius muscle) of the vibration pad on the patterns of wave propagation (wave image). When the vibration pad was placed in the trapezius muscle, the wave images represented clear wave propagation. On the other hand, when the vibration pad was placed in the head of the humerus, the wave images represented unclear wave propagation. This result might be caused by wave interferences resulting from the vibrations from bones and an intramuscular tendon of the supraspinatus muscle. The mean shear modulus also was 8.12 ± 1.83 (mean ± SD) kPa, when the vibration pad was placed in the trapezius muscle. Our results demonstrated that the vibration pad should be placed in the trapezius muscle in the MRE of the supraspinatus muscle.

We developed a Magnetic Resonance elastography (MRE) technique using a conventional magnetic resonance imaging (MRI), which allows a simultaneous elastography of the supraspinatus and trapezius muscles, by designing a new wave transducer (vibration pad) and optimizing the mechanical vibration frequency. Five healthy volunteers underwent an MRE. In order to transmit the mechanical vibration (pneumatic vibration) to the supraspinatus and trapezius muscles, a new vibration pad was designed using a three-dimensional (3D) printer. The vibration pad was placed on the skin 2 cm medial and 2 cm cephalad the deltoid tubercle. MRE acquisition was performed with a multi-slice gradient-echo type multi-echo MR sequence, which allows MREs even in a conventional MRI; two oblique axial images of the supraspinatus and trapezius muscles were obtained simultaneously. Vibration frequencies were set at 50–150 Hz, with a 25 Hz step. Wave image quality in each frequency was analyzed using a phase-to-noise ratio (PNR) and clarity of propagating wave that was assessed by two readers qualitatively. In the supraspinatus muscle, the wave images were of good quality especially at frequencies >75 Hz. In the trapezius muscle, the wave images were of better quality at low frequencies (50 and 75 Hz) compared with high frequencies (100–150 Hz). The PNR of both muscles were higher at low frequencies. The mean stiffness in the trapezius muscle (7.26 ± 2.13 kPa at 75 Hz) was larger than those in the supraspinatus muscle (4.16 ± 0.50 kPa at 75 Hz). The results demonstrated that our MRE technique allows simultaneous assessment of the stiffness in the supraspinatus and trapezius muscles using a conventional MRI, and that optimal vibration frequency for simultaneous MRE of these muscles is 75 Hz. This technique provides a new means for early detection of abnormality in the shoulder.

Diffusion-magnetic resonance elastography (dMRE) is an emerging practical technique that can acquire diffusion magnetic resonance imaging and MRE simultaneously. However, a signal loss attributable to intravoxel phase dispersion (IVPD) interferes with the calculation of the apparent diffusion coefficient (ADC). This study presents an approach to dMRE that reduces the influence of IVPD by introducing a new pulse sequence. The existing and proposed techniques were performed using a phantom comprising five rods with different elasticities at 60 Hz vibration to investigate the accuracy of previous and proposed dMRE techniques. The measures of ADC and stiffness, obtained by using both dMRE techniques, were compared with conventional spin-echo (SE) diffusion and SE-MRE. Then, we evaluated those differences by using the mean of absolute differences (MAD) in each rod within the phantom. The results of the MAD of the stiffness from both dMRE techniques showed almost no difference. In contrast, the value of the ADC MAD (MAD ≒ 0.16 × 10−3 mm2/s), obtained in the soft region within the phantom with the previous dMRE technique, was large. This value was about 2.7 times that of the value produced by the proposed dMRE technique. This difference must reflect the degree of influence of IVPD in both techniques. These results demonstrate that our dMRE technique is a robust method for addressing the signal loss attributable to IVPD.

For security, boards containing lead are commonly used to partition off rooms adjacent to radiation facilities. From the viewpoints of environmental safety and of material recycling during the process of remodeling or repair, however, the trend is toward avoiding the use of lead-containing radiation-protection board. Consequently, we developed a lead-free board(W 910xH 1820xD 12.5 mm)composed of a mixed material incorporating calcium sulfate and barium sulfate to be used as a partition at a diagnostic X-ray institution. The mass attenuation coefficients of the lead-free board were lower by about 12% and 60% for energy ranges of 25 approximately 37 keV and 38 approximately 41 keV, respectively, as compared with those of lead. Lead equivalents for the newly developed board(12.5 mm thickness)were 0.87+/-0.01 mmPb at 100 kV tube voltage, 0.60+/-0.01 at 125 kV, and 0.47+/-0.01 at 150 kV. A double-layer lead-free board proved to have enough shielding ability even at usual photographic frequencies.

It is important to evaluate the elasticity of tissue-engineered cartilage, for evaluating its structural strength. However, in vivo evaluation of tissue-engineered cartilage elasticity has not been established. On the other hand, since the speed of sound (SOS) is available for elasticity evaluation, we have proposed in vivo measurement method of SOS using ultrasound and MR images. This method determines the SOS based on the thickness measurement using the MR image and the time-of-flight (TOF) measurement using the ultrasound. In this study, this method was applied to the SOS measurement in tissue-engineered cartilage, and then relation between in vivo SOS and elasticity was investigated. As the result, in vivo SOS had a high correlation with Young's modulus. This result reveals that in vivo evaluation of tissue-engineered cartilage elasticity is available through in vivo SOS measurement.

BACKGROUND Transmission and tomographic X-ray measurements are useful in assessing bone structures, but only a few studies have examined cartilage growth because of the poor contrast in conventional X-ray imaging. OBJECTIVE In this study, we attempted to use the linear attenuation coefficient (LAC) as a metric of tissue-engineered cartilage development, which would be useful in high-throughput screening of cartilage products. METHODS Assuming that the LAC is related to the amount of extracellular matrix (ECM) in terms of the density and its atomic components, we measured X-ray absorption through tissue-engineered cartilage constructs. Characteristic X-ray beams from a molybdenum microfocus X-ray tube were employed to avoid beam hardening. The correlation of the LAC with mechanical properties was analyzed for verification. RESULTS The LAC was higher for chondrocyte constructs and lower for fibroblast-dominant constructs and was consistent with the quantification of toluidine blue staining, which is a proof of ECM production. The LAC was positively correlated with the bending modulus but negatively correlated with the dynamic elastic modulus and stiffness, possibly because of the remaining scaffold. CONCLUSIONS The LAC has the potential to be used as a metric of development of tissue-engineered cartilage. However, the calcified regions should be excluded from analysis to avoid decreasing the correlation between the LAC and the amount of ECM.

In order to detect the disease and degeneration of cartilage early, evaluations of acoustic properties are expected, but the physical meaning should be clarified. On the other hand, we have proposed a multimodal method using ultrasound (US) and magnetic resonance (MR) images for in vivo SOS measurement. In this study, the method was applied for the tissue-engineered cartilage, and the SOS was compared with T1, T2, apparent diffusion coefficient (ADC), attenuation coefficient (ATT), and Young’s modulus (YM). All procedures were approved by the Animal Care and Use Committee of our institution. 12-week-old male rats were used, and five different types of tissue-engineered cartilage was subcutaneously implanted in the back of rat (n = 30). Under anesthesia, MR and US images including same cross-sections were acquired separately, by using a MR imager of 2 Tesla and a US device of 13 MHz. At this time, T1, T2, ADC were also measured. The SOS was determined by the thickness measurement using the MR image and the...

The purpose of this study was to develop a new technique for a powerful compact MR elastography (MRE) actuator based on a pneumatic ball-vibrator. This is a compact actuator that generates powerful centrifugal force vibrations via high speed revolutions of an internal ball using compressed air. This equipment is easy to handle due to its simple principles and structure. Vibration frequency and centrifugal force are freely adjustable via air pressure changes (air flow volume), and replacement of the internal ball. In order to achieve MRI compatibility, all parts were constructed from non-ferromagnetic materials. Vibration amplitudes (displacements) were measured optically by a laser displacement sensor. From a bench test of displacement, even though the vibration frequency increased, the amount of displacement did not decrease. An essential step in MRE is the generation of mechanical waves within tissue via an actuator, and MRE sequences are synchronized to several phase offsets of vibration. In this system, the phase offset was detected by a four-channel optical-fiber sensor, and it was used as an MRI trigger signal. In an agarose gel phantom experiment, this actuator was used to make an MR elastogram. This study shows that the use of a ball actuator for MRE is feasible.

The purpose of this study was to examine the potential of diffusion-weighted (DW) three-dimensional (3D) MP-RAGE sequence for cerebral ischemia of a rat brain in vivo. The prototype of a DW-3D-MP-RAGE (3D-DWI) sequence was implemented at 2.0T animal experiment MR imager. The advantage of 3D imaging are that you obtain thinner and more slices with better profiles, and better signal-to-noise ratio for an equivalent slice thickness. Since 3D imaging is preferable for obtaining contiguous thin-slice MR images, image data with 3D imaging are expected to volume estimate of imaging area. Three-dimensional calculated absolute apparent diffusion coefficient (ADC) maps were compared with coronal stained (triphenyltetrazolium chloride: TTC) brain sections. From rat experimental results, the region of cerebral ischemia with 3D-DWI and the region of stained brain sections were well in agreement. This technique enables three-dimensional image display of a cerebral ischemia area, and makes it possible to measure the volume of a precise cerebral ischemia area in vivo., Date of Conference: Oct. 30 2010-Nov. 6 2010

Applying tissue-engineered cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of the cartilage. Magnetic resonance imaging (MRI) of articular cartilage has been widely accepted and applied clinically in recent years. In this study, we evaluated the negative fixed-charge density (nFCD) of tissue-engineered cartilage using gadolinium-enhanced MRI and determined the relationship between nFCD and biomechanical properties. To reconstruct cartilage tissue, articular chondrocytes from bovine humeral heads were embedded in agarose gel and cultured in vitro for up to 4 weeks. The nFCD of the cartilage was determined using the MRI gadolinium exclusion method. The equilibrium modulus was determined using a compressive stress relaxation test, and the dynamic modulus was determined by a dynamic compression test. The equilibrium compressive modulus and dynamic modulus of the tissue-engineered cartilage increased with an increase in culture time. The nFCD value—as determined with the [Gd-DTPA2−] measurement using the MRI technique—increased with culture time. In the regression analysis, nFCD showed significant correlations with equilibrium compressive modulus and dynamic modulus. From these results, gadolinium-enhanced MRI measurements can serve as a useful predictor of the biomechanical properties of tissue-engineered cartilage.

The application of tissue-engineered cartilage in a clinical setting requires a noninvasive method to assess the biophysical and biochemical properties of the engineered cartilage. Since articular cartilage is composed of 70-80% water and has dense extracellular matrixes (ECM), it is considered that the condition of the water molecules in the tissue is correlated with its biomechanical property. Therefore, magnetic resonance imaging (MRI) represents a potential approach to assess the biophysical property of the engineered cartilage. In this study, we test the hypothesis that quantitative MRI can be used as a noninvasive assessment method to assess the biophysical property of the engineered cartilage. To reconstruct a model of cartilaginous tissue, chondrocytes harvested from the humeral head of calves were embedded in an agarose gel and cultured in vitro up to 4 weeks. Equilibrium Young's moduli were determined from the stress relaxation tests. After mechanical testing, MRI-derived parameters (longitudinal relaxation time T1, transverse relaxation time T2, and water self-diffusion coefficient D) were measured. The equilibrium Young's modulus of the engineered cartilage showed a tendency to increase with an increase in the culture time, whereas T1 and D decreased. Based on a regression analysis, T1 and D showed a strong correlation with the equilibrium Young's modulus. The results showed that T1 and D values derived from the MRI measurements could be used to noninvasively monitor the biophysical properties of the engineered cartilage.

High b-value diffusion magnetic resonance imaging (MRI) enables us to detect far smaller architectures, by using q-space analysis, than the resolution in conventional MRI. Average displacement, one of the q-space parameters, quantitatively reflects architecture size and is very useful in observing small changes in microstructures in vivo (e.g., neurodegeneration, tumor heterogeneity, and others). Diffusion-weighted imaging (DWI) is performed by a two-dimensional (2D) multislice method; however, due to finite slice thickness and slice gap, there is a partial-volume effect that makes it difficult to detect the net q-space signal. On the other hand, three-dimensional (3D) MRI, having the advantages of very thin slice thickness and no slice gap (contiguous slices), allows volumetric evaluation acquired in a small isotropic voxel, as compared to 2D multislice imaging. Little is known about the isotropic high-resolution 3D DWI application to q-space analysis. In this study, we have developed and implemented a high b-value 3D DWI sequence, applied q-space analysis to study the reliability of high b-value 3D DWI and obtained a microscopic analytical map with isotropic high resolution and less contamination.

This paper discusses an MR Elastography (MRE) method for noninvasive measurement of elasticity and viscosity of biological tissues. In this imaging method, bipolar gradients put into any pulse sequence for MRI. The phase contrast of acquired image is changed by the number of bipolar gradients and their amplitude. From acquired signal (gradient echo and/or spin echo), changing of the phase of magnetized spin is calculated, and analyzed frequency response of applied mechanical vibration from the outside of the biological tissue. The frequency and amplitude of applied mechanical vibration should be changed by elasticity and viscosity of the tissues. The frequency of applied mechanical vibration is from around 100 Hz by mechanical vibrator to high around 1MHz by ultrasound device. In the post processing, elasticity and viscosity is calculated and the phase shift of magnetized spin is analyzed in two or three dimensions. An image is reconstructed as MRE image which is extracted and enhanced elasticity and viscosity of biological tissues.