1. Decellularized bone matrix/oleoyl chitosan derived supramolecular injectable hydrogel promotes efficient bone integration
- Author
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Amit Das, Santanu Dhara, Chandrika Gupta, Arun Prabhu Rameshbabu, Sayanti Datta, Madhurima Roy, Subhodeep Jana, Ramkrishna Sen, and Kamakshi Bankoti
- Subjects
Materials science ,Bone Regeneration ,Bone Matrix ,Bioengineering ,macromolecular substances ,02 engineering and technology ,Bone matrix ,010402 general chemistry ,Bone tissue ,complex mixtures ,01 natural sciences ,Biomaterials ,Chitosan ,Extracellular matrix ,chemistry.chemical_compound ,medicine ,Animals ,Bone regeneration ,Decellularization ,dBm ,technology, industry, and agriculture ,Hydrogels ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Extracellular Matrix ,medicine.anatomical_structure ,chemistry ,Mechanics of Materials ,Self-healing hydrogels ,Rabbits ,0210 nano-technology ,Biomedical engineering - Abstract
Hydrogels derived from decellularized extracellular matrix (ECM) have been widely used as a bioactive matrix for facilitating functional bone tissue regeneration. However, its poor mechanical strength and fast degradation restricts the extensive use for clinical application. Herein, we present a crosslinked decellularized bone ECM (DBM) and fatty acid modified chitosan (oleoyl chitosan, OC) based biohybrid hydrogel (DBM/OC) for delivering human amnion-derived stem cells (HAMSCs) for bone regeneration. DBM/OC hydrogel were benchmarked against collagen-I/OC (Col-I/OC) based hydrogel in terms of their morphological characteristics, rheological analysis, and biological performances. DBM/OC hydrogel with its endogenous growth factors recapitulates the nanofibrillar 3D tissue microenvironment with improved mechanical strength and also exhibited antimicrobial potential along with superior proliferation/differentiation ability. HAMSCs encapsulation potential of DBM/OC hydrogel was established by well spread cytoskeleton morphology post 14 days of cultivation. Further, ex-vivo chick chorioallantoic membrane (CAM) assay revealed excellent neovascularization potential of DBM/OC hydrogel. Subcutaneously implanted DBM/OC hydrogel did not trigger any severe immune response or infection in the host after 21 days. Also, DBM/OC hydrogels and HAMSCs encapsulated DBM/OC hydrogels were implanted at the tibial defect in a rabbit model to assess the bone regeneration ability. Quantitative micro-CT and histomorphological analysis demonstrated that HAMSCs encapsulated DBM/OC hydrogel can support more mature mineralized bone formation at the defect area compared to DBM/OC hydrogel or SHAM. These findings manifested the efficacy of DBM/OC hydrogel as a functional cell-delivery vehicle and osteoinductive template to accelerate bone regeneration.
- Published
- 2020