1. A small-volume thin-layer spectroelectrochemical cell for the study of biological components
- Author
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H. Brian Halsall, William R. Heineman, and C. William Anderson
- Subjects
Working electrode ,Materials science ,business.industry ,Small volume ,Thin layer ,Biophysics ,Analytical chemistry ,Cytochrome c Group ,Cell Biology ,Electrochemistry ,Biochemistry ,Redox ,Characterization (materials science) ,Coulometry ,Spectrophotometry ,Optoelectronics ,Gold ,Cyclic voltammetry ,business ,Electrodes ,Molecular Biology - Abstract
A small-volume (∼60 μl) spectroelectrochemical cell was constructed and characterized.The working electrode is An minigrid, and the spectral window is from 170 nm to 2 μm. The solution is held in a thin layer around the working electrode, providing the advantages of thin-layer electrochemistry. The cell can be constructed from common materials in about 6-h working time. The cell also has flow-through and vacuum capabilities, with only minor modifications. Characterization of the cell was done by cyclic voltammetry and coulometry on ferri-ferrocyanide and spectroelectrochemistry on cytochrome c and o-tolidine. The optical and electrochemical windows of this cell, as well as its convenient and versatile operation, make it useful for the study of a wide variety of biological redox components.
- Published
- 1979
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