37 results on '"Matsumoto, Kenji"'
Search Results
2. miR103a-3p in extracellular vesicles from FcεRI-aggregated human mast cells enhances IL-5 production by group 2 innate lymphoid cells.
- Author
-
Toyoshima S, Sakamoto-Sasaki T, Kurosawa Y, Hayama K, Matsuda A, Watanabe Y, Terui T, Gon Y, Matsumoto K, and Okayama Y
- Subjects
- Adult, Aged, Cells, Cultured, Dermatitis, Atopic immunology, Eosinophils immunology, Female, Humans, Immunity, Innate, Male, Middle Aged, Young Adult, Cytokines immunology, Extracellular Vesicles immunology, Lymphocytes immunology, Mast Cells immunology, MicroRNAs immunology, Receptors, IgE immunology
- Abstract
Background: Mast cells (MCs) are key regulators of IgE-mediated allergic inflammation. Cell-derived extracellular vesicles (EVs) contain bioactive compounds such as microRNAs. EVs can transfer signals to recipient cells, thus using a novel mechanism of cell-to-cell communication. However, whether MC-derived EVs are involved in FcεRI-mediated allergic inflammation is unclear., Objective: We sought to investigate the effect of EVs derived from FcεRI-aggregated human MCs on the function of human group 2 innate lymphoid cells (ILC2s)., Methods: Human cultured MCs were sensitized with and without IgE for 1 hour and then incubated with anti-IgE antibody, IL-33, or medium alone for 24 hours. EVs in the MC supernatant were isolated by using ExoQuick-TC., Results: Coculture of ILC2s with EVs derived from the FcεRI-aggregated MCs significantly enhanced IL-5 production and sustained upregulation of IL-5 mRNA expression in IL-33-stimulated ILC2s, but IL-13 production and IL-13 mRNA expression were unchanged. miR103a-3p expression was upregulated in IL-33-stimulated ILC2s that had been cocultured with EVs derived from anti-IgE antibody-stimulated MCs. Transduction of an miR103a-3p mimic to ILC2s significantly enhanced IL-5 production by IL-33-stimulated ILC2s. miR103a-3p promoted demethylation of an arginine residue of GATA3 by downregulating protein arginine methyltransferase 5 (PRMT5) mRNA. Reduction of protein arginine methyltransferase 5 expression in ILC2s by using a small interfering RNA technique resulted in upregulation of IL-5 production by IL-33-stimulated ILC2s. Furthermore, the level of miR103a-3p expression was significantly higher in EVs from sera of patients with atopic dermatitis than in EVs from nonatopic healthy control subjects., Conclusion: Eosinophilic allergic inflammation may be exacerbated owing to ILC2 activation by MC-derived miR103a-3p., (Copyright © 2021 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
3. Higher PGD 2 production by synovial mast cells from rheumatoid arthritis patients compared with osteoarthritis patients via miR-199a-3p/prostaglandin synthetase 2 axis.
- Author
-
Mishima S, Kashiwakura JI, Toyoshima S, Sasaki-Sakamoto T, Sano Y, Nakanishi K, Matsumoto K, and Okayama Y
- Subjects
- Aged, Aged, 80 and over, Arthritis, Rheumatoid genetics, Cells, Cultured, Cyclooxygenase 1 genetics, Cyclooxygenase 1 metabolism, Cyclooxygenase 2 genetics, Dinoprostone metabolism, Female, Fibroblasts metabolism, Gene Expression Profiling, Gene Expression Regulation, Histamine Release, Humans, Immunity, Innate, Interleukin-8 biosynthesis, Lymphocytes metabolism, Male, Middle Aged, Osteoarthritis genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, IgG metabolism, Synovial Fluid metabolism, Arthritis, Rheumatoid pathology, Cyclooxygenase 2 metabolism, Mast Cells metabolism, MicroRNAs metabolism, Osteoarthritis pathology, Prostaglandin D2 biosynthesis, Signal Transduction, Synovial Membrane pathology
- Abstract
We previously reported that synovial mast cells (MCs) from patients with rheumatoid arthritis (RA) produced TNF-α in response to immune complexes via FcγRI and FcγRIIA. However, the specific functions of synovial MCs in RA remain unclear. This study aimed to elucidate those functions. Synovial tissues and fluid were obtained from RA and osteoarthritis (OA) patients undergoing joint replacement surgery. Synovium-derived, cultured MCs were generated by culturing dispersed synovial cells with stem cell factor. We performed microarray-based screening of mRNA and microRNA (miRNA), followed by quantitative RT-PCR-based verification. Synovial MCs from RA patients showed significantly higher prostaglandin systhetase (PTGS)1 and PTGS2 expression compared with OA patients' MCs, and they produced significantly more prostaglandin D
2 (PGD2 ) following aggregation of FcγRI. PGD2 induced IL-8 production by human group 2 innate lymphoid cells, suggesting that PGD2 -producing MCs induce neutrophil recruitment into the synovium of RA patients. PTGS2 mRNA expression in RA patients' MCs correlated inversely with miRNA-199a-3p expression, which down-regulated PTGS2. RA patients' synovial fluid contained significantly more PGD2 compared with OA patients' fluid. Synovial MCs might regulate inflammation in RA through hyper-production of PGD2 following FcRγ aggregation. Our findings indicate functional heterogeneity of human MCs among diseases.- Published
- 2021
- Full Text
- View/download PDF
4. The basophil-IL-4-mast cell axis is required for food allergy.
- Author
-
Kashiwakura JI, Ando T, Karasuyama H, Kubo M, Matsumoto K, Matsuda T, and Kawakami T
- Subjects
- Disease Susceptibility, Humans, Basophils immunology, Basophils metabolism, Food Hypersensitivity immunology, Food Hypersensitivity metabolism, Interleukin-4 metabolism, Mast Cells immunology, Mast Cells metabolism
- Published
- 2019
- Full Text
- View/download PDF
5. IL-33 induces functional CCR7 expression in human mast cells.
- Author
-
Emi-Sugie M, Toyama S, Matsuda A, Saito H, and Matsumoto K
- Subjects
- Gene Expression Regulation, Humans, Transcriptome, Interleukin-33 immunology, Mast Cells immunology, Receptors, CCR7 genetics
- Published
- 2018
- Full Text
- View/download PDF
6. Regulatory roles of mast cells in immune responses.
- Author
-
Morita H, Saito H, Matsumoto K, and Nakae S
- Subjects
- Adaptive Immunity, Allografts immunology, Animals, Asthma etiology, Asthma metabolism, Dermatitis, Contact etiology, Dermatitis, Contact metabolism, Graft Rejection immunology, Graft Rejection metabolism, Graft vs Host Disease etiology, Graft vs Host Disease metabolism, Humans, Immunity, Innate, Immunoglobulin E immunology, Immunoglobulin E metabolism, Skin Transplantation adverse effects, Skin Transplantation methods, Immunity, Immunomodulation, Mast Cells immunology, Mast Cells metabolism
- Abstract
Mast cells are important immune cells for host defense through activation of innate immunity (via toll-like receptors or complement receptors) and acquired immunity (via FcεRI). Conversely, mast cells also act as effector cells that exacerbate development of allergic or autoimmune disorders. Yet, several lines of evidence show that mast cells act as regulatory cells to suppress certain inflammatory diseases. Here, we review the mechanisms by which mast cells suppress diseases.
- Published
- 2016
- Full Text
- View/download PDF
7. Activation of LXRs using the synthetic agonist GW3965 represses the production of pro-inflammatory cytokines by murine mast cells.
- Author
-
Nunomura S, Okayama Y, Matsumoto K, Hashimoto N, Endo-Umeda K, Terui T, Makishima M, and Ra C
- Subjects
- Animals, Cell Degranulation genetics, Cell Degranulation immunology, Cytokines genetics, Gene Expression, Immunoglobulin E blood, Immunoglobulin E immunology, Lipopolysaccharides immunology, Liver X Receptors, Mice, Mice, Knockout, Orphan Nuclear Receptors genetics, Orphan Nuclear Receptors metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, IgE agonists, Receptors, IgE metabolism, Benzoates pharmacology, Benzylamines pharmacology, Cytokines metabolism, Inflammation Mediators metabolism, Mast Cells drug effects, Mast Cells physiology, Orphan Nuclear Receptors agonists
- Abstract
Background: The activation of liver X receptor (LXR) α or LXRβ negatively regulates the expression of pro-inflammatory genes in mammalian cells. We recently reported that 25-hydroxycholesterol, a representative LXR-activating oxysterol, suppresses IL-6 production in mouse mast cells (MCs) following its engagement of the high-affinity IgE receptor (FcεRI). This finding suggests that murine MCs express functional LXRs; however, the mechanisms underlying the LXR-dependent repression of the MC-mediated production of pro-inflammatory cytokines, including IL-6, are poorly understood. Therefore, we employed the synthetic LXR ligand GW3965 to examine the functions of LXRα and LXRβ in the production of pro-inflammatory cytokines by murine bone marrow-derived MCs (BMMCs)., Methods: We prepared BMMCs from wild-type (WT), LXRα(-/-), and LXRα/β(-/-) mice. Each group of BMMCs was pretreated with GW3965 and then stimulated with IgE+antigen (Ag) or lipopolysaccharide (LPS). Cytokine production was then analyzed using specific ELISA kits., Results: The activation of LXRs by GW3965 significantly attenuated the production of IL-1α and IL-1β, but not of IL-6, in the WT and LXRα(-/-) BMMCs stimulated with IgE+Ag. However, GW3965 treatment decreased the production of IL-1α, IL-1β, and IL-6 in WT and LXRα(-/-) BMMCs upon stimulation with LPS, while the GW3965-mediated suppression of cytokine production was nearly absent from the LXRα/β(-/-) BMMCs., Conclusions: These findings demonstrate, for the first time, that the activation of LXRs by GW3965 attenuates the antigen- or LPS-induced production of pro-inflammatory cytokines, such as IL-1α and IL-1β, in murine MCs and that LXRβ plays an important role in the LXR-mediated repression of cytokine production., (Copyright © 2015 Japanese Society of Allergology. Production and hosting by Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
8. An Interleukin-33-Mast Cell-Interleukin-2 Axis Suppresses Papain-Induced Allergic Inflammation by Promoting Regulatory T Cell Numbers.
- Author
-
Morita H, Arae K, Unno H, Miyauchi K, Toyama S, Nambu A, Oboki K, Ohno T, Motomura K, Matsuda A, Yamaguchi S, Narushima S, Kajiwara N, Iikura M, Suto H, McKenzie AN, Takahashi T, Karasuyama H, Okumura K, Azuma M, Moro K, Akdis CA, Galli SJ, Koyasu S, Kubo M, Sudo K, Saito H, Matsumoto K, and Nakae S
- Subjects
- Animals, Bronchoalveolar Lavage Fluid cytology, Bronchoalveolar Lavage Fluid immunology, Cells, Cultured, Eosinophilia chemically induced, Humans, Interleukin-10 immunology, Interleukin-2 genetics, Interleukin-33, Interleukins genetics, Interleukins pharmacology, Lung cytology, Lung immunology, Lymphocyte Activation drug effects, Lymphocyte Activation immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Papain pharmacology, Proto-Oncogene Proteins c-kit genetics, Pyroglyphidae immunology, Th2 Cells immunology, Inflammation immunology, Interleukin-2 immunology, Interleukins immunology, Mast Cells immunology, T-Lymphocytes, Regulatory immunology
- Abstract
House dust mite-derived proteases contribute to allergic disorders in part by disrupting epithelial barrier function. Interleukin-33 (IL-33), produced by lung cells after exposure to protease allergens, can induce innate-type airway eosinophilia by activating natural helper (NH) cells, a member of group 2 innate lymphoid cells (ILC2), to secrete Th2 type-cytokines. Because IL-33 also can induce mast cells (MCs) to secrete Th2 type-cytokines, MCs are thought to cooperate with NH cells in enhancing protease or IL-33-mediated innate-type airway eosinophilia. However, we found that MC-deficient Kit(W-sh/W-sh) mice exhibited exacerbated protease-induced lung inflammation associated with reduced numbers of regulatory T (Treg) cells. Moreover, IL-2 produced by IL-33-stimulated MCs promoted expansion of numbers of Treg cells, thereby suppressing development of papain- or IL-33-induced airway eosinophilia. We have thus identified a unique anti-inflammatory pathway that can limit induction of innate-type allergic airway inflammation mediated by NH cells., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
9. Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
- Author
-
Inage E, Kasakura K, Yashiro T, Suzuki R, Baba Y, Nakano N, Hara M, Tanabe A, Oboki K, Matsumoto K, Saito H, Niyonsaba F, Ohtsuka Y, Ogawa H, Okumura K, Shimizu T, and Nishiyama C
- Subjects
- Cell Line, Cell Membrane metabolism, Chromatin Immunoprecipitation, GATA1 Transcription Factor genetics, GATA2 Transcription Factor genetics, Gene Knockdown Techniques, Humans, Promoter Regions, Genetic, Protein Binding, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-kit genetics, Proto-Oncogene Proteins c-kit metabolism, RNA Interference, RNA, Messenger genetics, RNA, Messenger metabolism, Trans-Activators genetics, Transcriptional Activation, GATA1 Transcription Factor metabolism, GATA2 Transcription Factor metabolism, Gene Expression Regulation, Mast Cells metabolism, Proto-Oncogene Proteins metabolism, Receptors, IgE genetics, Trans-Activators metabolism
- Abstract
The high-affinity IgE receptor, FcεRI, which is composed of α-, β-, and γ-chains, plays an important role in IgE-mediated allergic responses. In the current study, involvement of the transcription factors, PU.1, GATA1, and GATA2, in the expression of FcεRI on human mast cells was investigated. Transfection of small interfering RNAs (siRNAs) against PU.1, GATA1, and GATA2 into the human mast cell line, LAD2, caused significant downregulation of cell surface expression of FcεRI. Quantification of the mRNA levels revealed that PU.1, GATA1, and GATA2 siRNAs suppressed the α transcript, whereas the amount of β mRNA was reduced in only GATA2 siRNA transfectants. In contrast, γ mRNA levels were not affected by any of the knockdowns. Chromatin immunoprecipitation assay showed that significant amounts of PU.1, GATA1, and GATA2 bind to the promoter region of FCER1A (encoding FcεRIα) and that GATA2 binds to the promoter of MS4A2 (encoding FcεRIβ). Luciferase assay and EMSA showed that GATA2 transactivates the MS4A2 promoter via direct binding. These knockdowns of transcription factors also suppressed the IgE-mediated degranulation activity of LAD2. Similarly, all three knockdowns suppressed FcεRI expression in primary mast cells, especially PU.1 siRNA and GATA2 siRNA, which target FcεRIα and FcεRIβ, respectively. From these results, we conclude that PU.1 and GATA1 are involved in FcεRIα transcription through recruitment to its promoter, whereas GATA2 positively regulates FcεRIβ transcription. Suppression of these transcription factors leads to downregulation of FcεRI expression and IgE-mediated degranulation activity. Our findings will contribute to the development of new therapeutic approaches for FcεRI-mediated allergic diseases.
- Published
- 2014
- Full Text
- View/download PDF
10. Critical role for mast cell Stat5 activity in skin inflammation.
- Author
-
Ando T, Xiao W, Gao P, Namiranian S, Matsumoto K, Tomimori Y, Hong H, Yamashita H, Kimura M, Kashiwakura J, Hata TR, Izuhara K, Gurish MF, Roers A, Rafaels NM, Barnes KC, Jamora C, Kawakami Y, and Kawakami T
- Subjects
- Animals, Dermatitis, Atopic genetics, Gene Deletion, Humans, Mice, Mice, Inbred C57BL, Phospholipase C beta genetics, Polymorphism, Single Nucleotide, Protein Tyrosine Phosphatase, Non-Receptor Type 6 metabolism, STAT5 Transcription Factor genetics, Skin pathology, Dermatitis, Atopic metabolism, Mast Cells metabolism, Phospholipase C beta metabolism, STAT5 Transcription Factor metabolism, Skin metabolism
- Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease. Here, we show that phospholipase C-β3 (PLC-β3)-deficient mice spontaneously develop AD-like skin lesions and more severe allergen-induced dermatitis than wild-type mice. Mast cells were required for both AD models and remarkably increased in the skin of Plcb3(-/-) mice because of the increased Stat5 and reduced SHP-1 activities. Mast cell-specific deletion of Stat5 gene ameliorated allergen-induced dermatitis, whereas that of Shp1 gene encoding Stat5-inactivating SHP-1 exacerbated it. PLC-β3 regulates the expression of periostin in fibroblasts and TSLP in keratinocytes, two proteins critically involved in AD pathogenesis. Furthermore, polymorphisms in PLCB3, SHP1, STAT5A, and STAT5B genes were associated with human AD. Mast cell expression of PLC-β3 was inversely correlated with that of phospho-STAT5, and increased mast cells with high levels of phospho-STAT5 were found in lesional skin of some AD patients. Therefore, STAT5 regulatory mechanisms in mast cells are important for AD pathogenesis., (Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
11. Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line.
- Author
-
Kojima R, Ohno T, Iikura M, Niki T, Hirashima M, Iwaya K, Tsuda H, Nonoyama S, Matsuda A, Saito H, Matsumoto K, and Nakae S
- Subjects
- Cell Line, Cell Survival, Enzyme Activation, Humans, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 metabolism, Phosphorylation, Apoptosis, Cell Degranulation, Cytokines metabolism, Galectins metabolism, Mast Cells physiology
- Abstract
Galectin-9 (Gal-9), a lectin having a β-galactoside-binding domain, can induce apoptosis of Th1 cells by binding to TIM-3. In addition, Gal-9 inhibits IgE/Ag-mediated degranulation of mast cell/basophilic cell lines by binding to IgE, thus blocking IgE/Ag complex formation. However, the role of Gal-9 in mast cell function in the absence of IgE is not fully understood. Here, we found that recombinant Gal-9 directly induced phosphorylation of Erk1/2 but not p38 MAPK in a human mast cell line, HMC-1, which does not express FcεRI. Gal-9 induced apoptosis and inhibited PMA/ionomycin-mediated degranulation of HMC-1 cells. On the other hand, Gal-9 induced cytokine and/or chemokine production by HMC-1 cells, dependent on activation of ERK1/2 but not p38 MAPK. In addition, the lectin activity of Gal-9 was required for Gal-9-mediated cytokine secretion by HMC-1 cells. These observations suggest that Gal-9 has dual properties as both a regulator and an activator of mast cells.
- Published
- 2014
- Full Text
- View/download PDF
12. Mast cells are required for full expression of allergen/SEB-induced skin inflammation.
- Author
-
Ando T, Matsumoto K, Namiranian S, Yamashita H, Glatthorn H, Kimura M, Dolan BR, Lee JJ, Galli SJ, Kawakami Y, Jamora C, and Kawakami T
- Subjects
- Animals, Cell Differentiation, Dermatitis immunology, Dermatitis, Atopic immunology, Dermatitis, Atopic physiopathology, Eczema immunology, Enterotoxins pharmacology, Eosinophils cytology, Eosinophils immunology, Gene Expression Regulation, Homeodomain Proteins metabolism, Humans, Immunoglobulin E blood, Inflammation physiopathology, Lipid Metabolism, Mast Cells immunology, Mice, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis, Pyroglyphidae, Receptors, Antigen, T-Cell, alpha-beta metabolism, Skin immunology, T-Lymphocytes cytology, T-Lymphocytes immunology, Allergens immunology, Inflammation pathology, Mast Cells cytology, Skin pathology
- Abstract
Atopic dermatitis (AD) is a chronic pruritic inflammatory skin disease. We recently described an animal model in which repeated epicutaneous applications of a house dust mite extract and Staphylococcal enterotoxin B induced eczematous skin lesions. In this study we showed that global gene expression patterns are very similar between human AD skin and allergen/staphylococcal enterotoxin B-induced mouse skin lesions, particularly in the expression of genes related to epidermal growth/differentiation, skin barrier, lipid/energy metabolism, immune response, or extracellular matrix. In this model, mast cells and T cells, but not B cells or eosinophils, were shown to be required for the full expression of dermatitis, as revealed by reduced skin inflammation and reduced serum IgE levels in mice lacking mast cells or T cells (TCRβ(-/-) or Rag1(-/-)). The clinical severity of dermatitis correlated with the numbers of mast cells, but not eosinophils. Consistent with the idea that T helper type 2 (Th2) cells play a predominant role in allergic diseases, the receptor for the Th2-promoting cytokine thymic stromal lymphopoietin and the high-affinity IgE receptor, FcɛRI, were required to attain maximal clinical scores. Therefore, this clinically relevant model provides mechanistic insights into the pathogenic mechanism of human AD.
- Published
- 2013
- Full Text
- View/download PDF
13. Activation of human synovial mast cells from rheumatoid arthritis or osteoarthritis patients in response to aggregated IgG through Fcγ receptor I and Fcγ receptor II.
- Author
-
Lee H, Kashiwakura J, Matsuda A, Watanabe Y, Sakamoto-Sasaki T, Matsumoto K, Hashimoto N, Saito S, Ohmori K, Nagaoka M, Tokuhashi Y, Ra C, and Okayama Y
- Subjects
- Arthritis, Rheumatoid metabolism, Arthritis, Rheumatoid pathology, Cells, Cultured, Flow Cytometry, Humans, Immunohistochemistry, In Vitro Techniques, Mast Cells metabolism, Osteoarthritis metabolism, Osteoarthritis pathology, Receptors, IgG metabolism, Synovial Membrane metabolism, Arthritis, Rheumatoid immunology, Immunoglobulin G immunology, Mast Cells immunology, Osteoarthritis immunology, Receptors, IgG immunology, Synovial Membrane immunology
- Abstract
Objective: Substantial evidence suggests that human synovial mast cells (MCs) are involved in the pathogenesis of rheumatoid arthritis (RA). A plausible pathway for the activation of synovial MCs is through IgG receptors, given the prevalence of circulating IgG isotype autoantibodies and synovial immune complexes in patients with RA. However, IgG receptor expression on human synovial MCs remains uncharacterized. The aim of this study was to identify which IgG receptor(s) on synovial MCs are responsible for MC activation in immune complexes., Methods: Synovial tissue specimens were obtained from patients with RA or patients with osteoarthritis (OA) who were undergoing joint replacement surgery, and synovial MCs were enzymatically dispersed. Cultured synovium-derived MCs were generated by culturing synovial cells with stem cell factor, and receptor expression was analyzed using fluorescence-activated cell sorting. Mediators released from MCs were measured using enzyme immunoassays or enzyme-linked immunosorbent assays., Results: Primary synovial MCs and cultured synovium-derived MCs obtained from both patients with RA and patients with OA expressed Fcε receptor I (FcεRI), FcγRI, and FcγRII but not FcγRIII. Cultured synovium-derived MCs induced degranulation and the production of prostaglandin D2 and tumor necrosis factor α (TNFα) through FcγRI. The aggregation of FcγRII caused histamine release from cultured MCs but not from primary MCs. Histamine release induced by aggregated IgG was significantly inhibited by neutralizing anti-FcγRI monoclonal antibody and anti-FcγRII monoclonal antibody., Conclusion: With regard to the FcR expression profile, synovial MCs from patients with RA and patients with OA were similar. FcγRI was responsible for producing abundant TNFα from synovial MCs in response to aggregated IgG. Immune complexes may activate synovial MCs through FcγRI and FcγRII., (Copyright © 2013 by the American College of Rheumatology.)
- Published
- 2013
- Full Text
- View/download PDF
14. Omalizumab inhibits acceleration of FcεRI-mediated responsiveness of immature human mast cells by immunoglobulin E.
- Author
-
Okayama Y, Kashiwakura J, Sasaki-Sakamoto T, Matsumoto K, Hashimoto N, Ohmori K, Kawakami T, Saito H, and Ra C
- Subjects
- Asthma pathology, Cells, Cultured, Gene Expression, Histamine Release, Humans, Immunoglobulin E metabolism, Mast Cells metabolism, Omalizumab, RNA, Messenger biosynthesis, Receptors, IgE biosynthesis, Antibodies, Anti-Idiotypic pharmacology, Antibodies, Monoclonal, Humanized pharmacology, Immunoglobulin E immunology, Mast Cells immunology, Receptors, IgE immunology
- Abstract
Background: A large body of evidence has demonstrated that treatment with omalizumab is clinically effective for the management of moderate to severe allergic asthma, emphasizing the importance of IgE in the pathogenesis of allergic asthma. We hypothesized that IgE accelerates FcεRI-mediated responsiveness of "immature" human mast cells (MCs) and that omalizumab downregulates the acceleration., Objectives: To examine when MC progenitors acquired the ability to degranulate following FcεRI aggregation, whether IgE accelerates the responsiveness of immature MCs following FcεRI aggregation, and whether omalizumab regulates such an acceleration., Methods: Gene expression was examined using a microarray and quantitative reverse transcription polymerase chain reaction. Protein expression was investigated using FACS. Histamine release was examined using an EIA., Results: The time-course analysis of the mRNA expression of MC-related genes, including FcεRI, in Kit(+) sorted cells during the differentiation and histamine experiments revealed that the expression level of FcεRI in 5 week (w)-cultured MCs was not sufficient to induce degranulation following FcεRI aggregation but that 5 w-cultured MCs were fully responsive to calcium ionophore. By addition of IgE in culture medium FcεRI expression level and FcεRI-mediated histamine release of 5 w-cultured MCs were significantly increased compared with those without addition of IgE, whereas the expression level of tryptase and number of MCs was not affected. Omalizumab significantly inhibited IgE-dependent enhancement of FcεRI expression level and FcεRI-mediated histamine release., Conclusions: High levels of IgE in the microenvironment in vivo may upregulate the responsiveness of immature MCs to allergens. Omalizumab may inhibit the IgE-mediated responsiveness of not only mature MCs, but also immature MCs., (Copyright © 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
15. [Role of mast cells in allergy].
- Author
-
Saito H, Kato A, and Matsumoto K
- Subjects
- Airway Remodeling physiology, Humans, Hypersensitivity, Immediate physiopathology, Immunoglobulin E physiology, Hypersensitivity physiopathology, Mast Cells physiology
- Abstract
Mast cells play a central role in IgE-mediated immediate-type allergic reaction and contribute to the late-phase allergic inflammation by releasing a variety of cytokines and chemokines. These cells also have a substantial effect on tissue remodeling, especially on airway smooth muscle hypertrophy and mucus hypersecretion by releasing proteases and growth factors such as tryptase and amphiregulin. The activation of mast cells in vitro is partially inhibited by the pretreatment with corticosteroid or tacrolimus through inhibition of NF-kappaB and NF-AT, respectively, and is profoundly blocked by the simultaneous treatment of the two drugs. Pharmaceutical development of safer drugs is expected to treat mast cell-mediated allergic disorders.
- Published
- 2009
16. Dexamethasone and FK506 inhibit expression of distinct subsets of chemokines in human mast cells.
- Author
-
Kato A, Chustz RT, Ogasawara T, Kulka M, Saito H, Schleimer RP, and Matsumoto K
- Subjects
- Adrenal Cortex Hormones, Anti-Inflammatory Agents pharmacology, Antibodies pharmacology, Calcineurin Inhibitors, Cells, Cultured, Chemokines, CC genetics, Chemokines, CXC genetics, Drug Therapy, Combination, Gene Expression Profiling, Gene Expression Regulation immunology, Humans, Immunoglobulin E immunology, Immunosuppressive Agents pharmacology, Mast Cells drug effects, Receptors, IgE physiology, Chemokines genetics, Dexamethasone pharmacology, Gene Expression Regulation drug effects, Mast Cells metabolism, Tacrolimus pharmacology
- Abstract
Mast cells produce a large amount of several chemokines after cross-linking of FcepsilonRI and participate in the pathogenesis of allergic diseases. The objective of this study was to comprehensively investigate FcepsilonRI-mediated chemokine induction in human mast cells and the effect of a corticosteroid (dexamethasone) and a calcineurin inhibitor (FK506). Human peripheral blood-derived mast cells were stimulated with anti-IgE Ab in the presence of dexamethasone or FK506. Gene expression profiles were evaluated using GeneChip and confirmed by real-time PCR, and chemokine concentrations were measured by cytometric bead arrays and ELISA. Expression of eight chemokines was significantly induced in mast cells by anti-IgE stimulation. Induction of CCL2, CCL7, CXCL3, and CXCL8 by anti-IgE was significantly inhibited by dexamethasone but was enhanced by FK506. In contrast, induction of CCL1, CCL3, CCL4, and CCL18 was significantly inhibited by FK506 but, with the exception of CCL1, was enhanced by dexamethasone. Combination of dexamethasone and FK506 suppressed production of all chemokines by anti-IgE stimulation. Studies using protease inhibitors indicate that mast cell proteases may degrade several of the chemokines. These results suggest that corticosteroids and calcineurin inhibitors inhibit expression of distinct subsets of chemokines, and a combination of these drugs almost completely suppresses the induction of all chemokine genes in human mast cells in response to FcepsilonRI-dependent stimulation. This implies that a combination of a corticosteroid and a calcineurin inhibitor may be more effective than each single agent for the treatment of allergic diseases in which mast cell-derived chemokines play a major role.
- Published
- 2009
- Full Text
- View/download PDF
17. Advances in mechanisms of asthma, allergy, and immunology in 2008.
- Author
-
Boyce JA, Broide D, Matsumoto K, and Bochner BS
- Subjects
- Animals, Antibodies, Anti-Idiotypic, Antibodies, Monoclonal immunology, Antibodies, Monoclonal therapeutic use, Antibodies, Monoclonal, Humanized, Asthma genetics, Asthma therapy, Basophils metabolism, Cell Degranulation immunology, Eosinophils metabolism, Genetic Predisposition to Disease, Humans, Hypersensitivity genetics, Hypersensitivity therapy, Immunoglobulin E blood, Inflammation Mediators metabolism, Mast Cells metabolism, Omalizumab, Asthma immunology, Basophils immunology, Eosinophils immunology, Hypersensitivity immunology, Inflammation Mediators immunology, Mast Cells immunology
- Abstract
This review summarizes selected articles appearing in 2008 in the Journal. Articles chosen include those improving our understanding of mechanisms of allergic diseases by focusing on human basophil, mast cell, and eosinophil biology; IgE and its high-affinity receptor on various cells; novel properties of omalizumab; airways remodeling; and genetics. Articles from other journals have been included to supplement the topics presented.
- Published
- 2009
- Full Text
- View/download PDF
18. Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
- Author
-
Shimizu Y, Matsumoto K, Okayama Y, Sakai K, Maeno T, Suga T, Miura T, Takai S, Kurabayashi M, and Saito H
- Subjects
- Antigens, CD34 metabolism, Cells, Cultured, Flow Cytometry, Humans, Interleukin-6 metabolism, Oligonucleotide Array Sequence Analysis, Stem Cell Factor metabolism, Stem Cells cytology, Bone Marrow Cells cytology, Cell Differentiation drug effects, Interleukin-3 pharmacology, Mast Cells cytology, Mast Cells drug effects
- Abstract
Although IL-3 is commonly used for culture of human progenitor-derived mast cells together with Stem cell factor (SCF) and IL-6, the effect of IL-3 on human mast cell differentiation has not been well elucidated. Human bone marrow CD34+ progenitors were cultured for up to 12 weeks in the presence of rhSCF and rhIL-6 either with rhIL-3 (IL-3 (+)) or without rhIL-3 (IL-3 (-)) for the initial 1-week of culture. Total cell number increased at 2 weeks in IL-3 (+), as compared to IL-3 (-), but changes in the appearance of mast cells were delayed. When IL-3 was present for the initial 1-week culture, granules looked more mature with IL-3 than without IL-3. However, tryptase and chymase contents, and surface antigen expression (CD18, CD51, CD54, and CD117) were not altered by IL-3. Surface expression and mRNA level of FcepsilonRIalpha and histamine release by crosslinking of FcepsilonRIalpha did not differ from one preparation to the next. GeneChip analysis revealed that no significant differences were observed between IL-3 (+) and IL-3 (-) cells either when inactivated or activated by aggregation of FcepsilonRIalpha. These findings indicate that initial incubation of human bone marrow CD34+ progenitors with IL-3 does not affect the differentiation of mast cells.
- Published
- 2008
- Full Text
- View/download PDF
19. Antimicrobial peptides human beta-defensin (hBD)-3 and hBD-4 activate mast cells and increase skin vascular permeability.
- Author
-
Chen X, Niyonsaba F, Ushio H, Hara M, Yokoi H, Matsumoto K, Saito H, Nagaoka I, Ikeda S, Okumura K, and Ogawa H
- Subjects
- Animals, Blotting, Western, Chemotaxis, Leukocyte immunology, Extracellular Signal-Regulated MAP Kinases metabolism, Humans, Phosphorylation, Rats, Skin blood supply, beta-Defensins metabolism, p38 Mitogen-Activated Protein Kinases metabolism, Capillary Permeability immunology, Mast Cells immunology, Skin immunology, beta-Defensins immunology
- Abstract
Antimicrobial peptides human beta-defensins (hBD) are mainly produced by epithelia of several organs including skin, and participate in innate immunity by killing invading pathogens. Besides their microbicidal activities, hBD activate several inflammatory and immune cells. Since hBD are generated by tissues where mast cells are present, we hypothesized that these peptides could activate mast cells. In this study, we demonstrated that both hBD-3 and hBD-4 induced mast cell degranulation, prostaglandin D2 production, intracellular Ca2+ mobilization and chemotaxis. Furthermore, hBD-3- and hBD-4-induced activation of mast cells was suppressed by pertussis toxin and U-73122, inhibitors for G protein and phospholipase C, respectively. We further revealed that hBD-3 and hBD-4 increased vascular permeability in the skin, which was dependent on the presence of mast cells, because hBD-3 and hBD-4 failed to enhance vascular permeability in mast cell-deficient Ws/Ws rats. We also demonstrated that hBD-3 and hBD-4 induced phosphorylation of MAPK p38 and ERK1/2, which were further required for hBD-mediated mast cell activation, as evidenced by the inhibitory effects of p38 and ERK1/2 inhibitors on mast cell degranulation. Together, these findings suggest the key role of hBD in inflammatory responses by recruiting and activating mast cells, and increasing vascular permeability.
- Published
- 2007
- Full Text
- View/download PDF
20. Gene expression profiling of human mast cell subtypes: an in silico study.
- Author
-
Saito H, Matsumoto K, Okumura S, Kashiwakura J, Oboki K, Yokoi H, Kambe N, Ohta K, and Okayama Y
- Subjects
- Cells, Cultured, Chymases biosynthesis, Chymases genetics, Humans, Mast Cells enzymology, Multigene Family, Computational Biology methods, Gene Expression Profiling methods, Gene Expression Regulation immunology, Mast Cells chemistry, Mast Cells classification, Oligonucleotide Array Sequence Analysis
- Abstract
Background: Human mast cells (MCs) were classified into at least two subtypes, i.e., tryptase- and chymase-positive MCs (MC(TC)) and tryptase-only-positive MCs (MC(T)). However, differences in global molecular expression between these subtypes are unknown., Methods: We analyzed public microarray data of MC subtypes derived from various tissues and those of peripheral blood granulocytes by using hierarchical clustering methods to understand the global gene expression profiles., Results: All the transcripts subjected to this clustering analysis were classified into two large clusters, i.e., MC-preferential or granulocyte-preferential. In the original works, MCs from tonsil, lung and skin had been cultured for more than several weeks to obtain highly viable and pure cell populations, and these MCs retained their typical profiles such as intensities of chymase protein expression. Most of the transcripts were commonly expressed by these MC subtypes. However, tonsil-derived MCs and skin-derived MCs but not lung-derived MCs expressed high levels of chymase (CMA1) as expected for the properties of MC(TC) and MC(T). These CMA1-high MCs and CMA1-low MCs respectively expressed distinct sets of transcripts as small gene clusters as well as CMA-1 even after being cultured in the absence of a tissue environment., Conclusions: The MC lineage seems to be far from the granulocyte lineages including basophils. CMA1-high MCs (MC(TC)) and CMA1-low MCs (MC(T)) can be regarded as differentiated MC subtypes. As such, importance of data analysis studies will be increasing along with the accumulation of global molecular data in the public database.
- Published
- 2006
- Full Text
- View/download PDF
21. Culture of human mast cells from peripheral blood progenitors.
- Author
-
Saito H, Kato A, Matsumoto K, and Okayama Y
- Subjects
- Humans, Cell Culture Techniques, Cell Separation methods, Hematopoietic Stem Cells, Mast Cells
- Abstract
This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10(4)-10(5) cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10(3)-10(4) mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10(6)-10(7).
- Published
- 2006
- Full Text
- View/download PDF
22. Identification of specific gene expression profiles in human mast cells mediated by Toll-like receptor 4 and FcepsilonRI.
- Author
-
Okumura S, Kashiwakura J, Tomita H, Matsumoto K, Nakajima T, Saito H, and Okayama Y
- Subjects
- Chemokine CCL1, Chemokines, CC metabolism, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation drug effects, Gene Expression Regulation immunology, Humans, Interleukin-5 metabolism, Lipopolysaccharides pharmacology, Lung cytology, Membrane Glycoproteins genetics, Monocytes cytology, Monocytes physiology, Receptors, Cell Surface genetics, Receptors, IgE genetics, Toll-Like Receptor 4, Toll-Like Receptors, Tumor Necrosis Factor-alpha biosynthesis, Gene Expression Profiling, Mast Cells physiology, Membrane Glycoproteins metabolism, Receptors, Cell Surface metabolism, Receptors, IgE metabolism
- Abstract
Rodent mast cells (MCs) are reported to play a pivotal role in both innate and adaptive immunity. However, there is so far no evidence that human MCs are involved in innate immunity. We found that a functional Toll-like receptor 4 (TLR4) was expressed on human MCs when it was up-regulated by interferon gamma (IFN-gamma). To systematically explore how human MCs modulate the immune system following TLR4-mediated activation and FcepsilonRI aggregation, we used high-density oligonucleotide probe arrays (GeneChip) to compare the lipopolysaccharide (LPS)-induced gene expression profile with the IgE/anti-IgE-mediated profile in MCs. Both a shared core response, and LPS- or anti-IgE-specific programs of gene expression were observed in MCs. Furthermore, MCs exhibited an antiviral response gene program in response to IFN-gamma, and LPS sustained that expression. Compared with the LPS-stimulated gene expression profile of human peripheral blood mononuclear cells, LPS-stimulated MCs specifically induced a subset of genes that included a Th2 cytokine and chemokines that recruit Th2 cells and eosinophils. These results reveal that human MCs express tailored pathogen- and antigen-specific immune responses and that human MCs may play important roles in innate and adaptive immunity.
- Published
- 2003
- Full Text
- View/download PDF
23. Marked increase in CC chemokine gene expression in both human and mouse mast cell transcriptomes following Fcepsilon receptor I cross-linking: an interspecies comparison.
- Author
-
Nakajima T, Inagaki N, Tanaka H, Tanaka A, Yoshikawa M, Tamari M, Hasegawa K, Matsumoto K, Tachimoto H, Ebisawa M, Tsujimoto G, Matsuda H, Nagai H, and Saito H
- Subjects
- Animals, Antigens, CD, Chemokine CCL1, Chemokine CCL3, Chemokine CCL4, Chemokines, CC biosynthesis, Humans, Macrophage Inflammatory Proteins genetics, Mice, Mice, Inbred BALB C, RNA, Messenger analysis, RNA, Messenger biosynthesis, Receptors, IgE physiology, Receptors, Nerve Growth Factor genetics, Receptors, Tumor Necrosis Factor genetics, Tumor Necrosis Factor Receptor Superfamily, Member 9, Up-Regulation, Chemokines, CC genetics, Gene Expression Profiling, Mast Cells metabolism, Receptors, IgE metabolism
- Abstract
Rodent mast cells (MCs) are common experimental tools but are somewhat different from their human counterparts in their responses to certain cytokines and drugs. We examined the expression of more than 10 000 distinct genes in human and mouse cultured MCs using high-density oligonucleotide probe arrays to find molecules similarly regulated and expressed by the 2 MC types. After stimulation via high-affinity Fcepsilon receptor I (FcepsilonRI), the transcriptional levels of several CC chemokines were markedly increased, and I-309 (CCL1), macrophage inflammatory protein-1alpha (MIP-1alpha) (CCL3) and MIP-1beta (CCL4) were found among the 10 most increased human and mouse transcripts from approximately 12 000 genes (including some expressed sequence tags). In addition, a costimulatory molecule that was originally found on the membrane of activated T cells, 4-1BB (CD137), was found among the 10 most increased transcripts. The FcepsilonRI-induced expression of CC chemokines and 4-1BB was also detected at the protein level in both MC types. The conservation of these responses suggests that MCs play a crucial role in recruitment of various CCR-expressing cells into the tissue in a manner dependent on immunoglobin E, and that FcepsilonRI-mediated induction of several CC chemokines and 4-1BB is highly conserved between human and mouse. Interspecies comparison studies at the whole genome expression level should be useful for the interpretation of experimental data obtained in animal models of human pathobiology.
- Published
- 2002
- Full Text
- View/download PDF
24. The Prostaglandin D2 Receptor CRTH2 Contributes to Airway Hyperresponsiveness during Airway Inflammation Induced by Sensitization without an Adjuvant in Mice.
- Author
-
Hanzawa, Satoshi, Sugiura, Makiko, Nakae, Susumu, Masuo, Masahiro, Morita, Hideaki, Matsumoto, Kenji, Takeda, Kazuyoshi, Okumura, Ko, Nakamura, Masataka, Ohno, Tatsukuni, and Miyazaki, Yasunari
- Subjects
TH2 cells ,INNATE lymphoid cells ,MAST cells ,PROSTAGLANDIN receptors ,LEUKOCYTE count - Abstract
Introduction: Prostaglandin D2 (PGD2), which is produced mainly by Th2 cells and mast cells, promotes a type-2 immune response by activating Th2 cells, mast cells, eosinophils, and group 2 innate lymphoid cells (ILC2s) via its receptor, chemoattractant receptor-homologous molecules on Th2 cells (CRTH2). However, the role of CRTH2 in models of airway inflammation induced by sensitization without adjuvants, in which both IgE and mast cells may play major roles, remain unclear. Methods: Wild-type (WT) and CRTH2-knockout (KO) mice were sensitized with ovalbumin (OVA) without an adjuvant and then challenged intranasally with OVA. Airway inflammation was assessed based on airway hyperresponsiveness (AHR), lung histology, number of leukocytes, and levels of type-2 cytokines in the bronchoalveolar lavage fluid (BALF). Results: AHR was significantly reduced after OVA challenge in CRTH2 KO mice compared to WT mice. The number of eosinophils, levels of type-2 cytokines (IL-4, IL-5, and IL-13) in BALF, and IgE concentration in serum were decreased in CRTH2 KO mice compared to WT mice. However, lung histological changes were comparable between WT and CRTH2 KO mice. Conclusion: CRTH2 is responsible for the development of asthma responses in a mouse model of airway inflammation that features prominent involvement of both IgE and mast cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
25. GATA-1 regulates the generation and function of basophils
- Author
-
Nei, Yuichiro, Obata-Ninomiya, Kazushige, Tsutsui, Hidemitsu, Ishiwata, Kenji, Miyasaka, Masayuki, Matsumoto, Kenji, Nakae, Susumu, Kanuka, Hirotaka, Inase, Naohiko, and Karasuyama, Hajime
- Published
- 2013
26. Legends of allergy and immunology: Hirohisa Saito.
- Author
-
Morita, Hideaki and Matsumoto, Kenji
- Subjects
- *
HUMAN cell culture , *ALLERGIES , *MAST cells , *IMMUNOLOGY - Abstract
Hirohisa Saito, a pediatrician turned researcher, made significant contributions to the field of allergy and immunology. During his time at Johns Hopkins University, he developed a method for culturing human mast cells, a process that had taken 18 years to achieve. After returning to Japan, Saito continued his research on mast cells and made advancements in understanding their gene expression. He also conducted clinical research and wrote several randomized controlled studies. Saito's contributions include establishing a culture method for human mast cells, elucidating their gene expression, studying the function of IL-33, and validating a preventive strategy for atopic march. [Extracted from the article]
- Published
- 2024
- Full Text
- View/download PDF
27. Higher PGD2 production by synovial mast cells from rheumatoid arthritis patients compared with osteoarthritis patients via miR-199a-3p/prostaglandin synthetase 2 axis.
- Author
-
Mishima, Shintaro, Kashiwakura, Jun-ichi, Toyoshima, Shota, Sasaki-Sakamoto, Tomomi, Sano, Yutaka, Nakanishi, Kazuyoshi, Matsumoto, Kenji, and Okayama, Yoshimichi
- Subjects
PROSTAGLANDINS ,RHEUMATOID arthritis ,MAST cells ,OSTEOARTHRITIS ,MICRORNA - Abstract
We previously reported that synovial mast cells (MCs) from patients with rheumatoid arthritis (RA) produced TNF-α in response to immune complexes via FcγRI and FcγRIIA. However, the specific functions of synovial MCs in RA remain unclear. This study aimed to elucidate those functions. Synovial tissues and fluid were obtained from RA and osteoarthritis (OA) patients undergoing joint replacement surgery. Synovium-derived, cultured MCs were generated by culturing dispersed synovial cells with stem cell factor. We performed microarray-based screening of mRNA and microRNA (miRNA), followed by quantitative RT-PCR-based verification. Synovial MCs from RA patients showed significantly higher prostaglandin systhetase (PTGS)1 and PTGS2 expression compared with OA patients' MCs, and they produced significantly more prostaglandin D
2 (PGD2 ) following aggregation of FcγRI. PGD2 induced IL-8 production by human group 2 innate lymphoid cells, suggesting that PGD2 -producing MCs induce neutrophil recruitment into the synovium of RA patients. PTGS2 mRNA expression in RA patients' MCs correlated inversely with miRNA-199a-3p expression, which down-regulated PTGS2. RA patients' synovial fluid contained significantly more PGD2 compared with OA patients' fluid. Synovial MCs might regulate inflammation in RA through hyper-production of PGD2 following FcRγ aggregation. Our findings indicate functional heterogeneity of human MCs among diseases. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
28. Dexamethasone and FK506 Inhibit Expression of Distinct Subsets of Chemokines in Human Mast Cells1
- Author
-
Kato, Atsushi, Chustz, Regina T., Ogasawara, Takahisa, Kulka, Marianna, Saito, Hirohisa, Schleimer, Robert P., and Matsumoto, Kenji
- Subjects
Receptors, IgE ,Gene Expression Profiling ,Calcineurin Inhibitors ,Anti-Inflammatory Agents ,Immunoglobulin E ,Article ,Antibodies ,Dexamethasone ,Tacrolimus ,Gene Expression Regulation ,Adrenal Cortex Hormones ,Chemokines, CC ,Humans ,Drug Therapy, Combination ,Mast Cells ,Chemokines ,Chemokines, CXC ,Cells, Cultured ,Immunosuppressive Agents - Abstract
Mast cells produce a large amount of several chemokines after cross-linking of FcepsilonRI and participate in the pathogenesis of allergic diseases. The objective of this study was to comprehensively investigate FcepsilonRI-mediated chemokine induction in human mast cells and the effect of a corticosteroid (dexamethasone) and a calcineurin inhibitor (FK506). Human peripheral blood-derived mast cells were stimulated with anti-IgE Ab in the presence of dexamethasone or FK506. Gene expression profiles were evaluated using GeneChip and confirmed by real-time PCR, and chemokine concentrations were measured by cytometric bead arrays and ELISA. Expression of eight chemokines was significantly induced in mast cells by anti-IgE stimulation. Induction of CCL2, CCL7, CXCL3, and CXCL8 by anti-IgE was significantly inhibited by dexamethasone but was enhanced by FK506. In contrast, induction of CCL1, CCL3, CCL4, and CCL18 was significantly inhibited by FK506 but, with the exception of CCL1, was enhanced by dexamethasone. Combination of dexamethasone and FK506 suppressed production of all chemokines by anti-IgE stimulation. Studies using protease inhibitors indicate that mast cell proteases may degrade several of the chemokines. These results suggest that corticosteroids and calcineurin inhibitors inhibit expression of distinct subsets of chemokines, and a combination of these drugs almost completely suppresses the induction of all chemokine genes in human mast cells in response to FcepsilonRI-dependent stimulation. This implies that a combination of a corticosteroid and a calcineurin inhibitor may be more effective than each single agent for the treatment of allergic diseases in which mast cell-derived chemokines play a major role.
- Published
- 2009
29. Pretreatment with Low Levels of FcεRI-Crosslinking Stimulation Enhances Basophil Mediator Release.
- Author
-
Koketsu, Rikiya, Yamaguchi, Masao, Suzukawa, Maho, Tanaka, Yusuke, Tashimo, Hiroyuki, arai, Hidenori, Nagase, Hiroyuki, Matsumoto, Kenji, Saito, Hirohisa, Ra, Chisei, Yamamoto, Kazuhiko, and Ohta, Ken
- Subjects
BASOPHILS ,MAST cells ,IMMUNOGLOBULIN E ,CELL culture ,ALLERGENS ,ALLERGIES - Abstract
Background: Basophils and mast cells are important initiator/effector cells capable of rapidly responding to IgE-mediated stimulation, but the precise mechanisms regulating their functions in vivo have not been fully identified. In this study, we assessed whether low levels of antigen can modulate activation of basophils and mast cells. Methods: Human basophils and cultured mast cells were pretreated with low concentrations of anti-FcεRI α-chain mAb (CRA-1 mAb), and their cell functions were assessed. Results: Basophils preincubated with CRA-1 mAb at as low as 1 ng/ml for 1 h showed significantly enhanced degranulation in response to various secretagogues such as MCP-1, FMLP, leukotriene B
4 and Ca ionophore A23187. FMLP-induced leukotriene C4 production by basophils was also enhanced by CRA-1 mAb pretreatment. Degranulation was further enhanced when CRA-1 mAb-pretreated basophils were additionally treated with IL-3, IL-33 or leptin before stimulation with MCP-1. Priming by subthreshold CRA-1 mAb was a slow process, since 1 h of pretreatment was needed for maximal enhancement. Basophil priming also resulted from preincubation with subthreshold doses of an allergen, Der f 2. In parallel mAb experiments, CRA-1 mAb showed weak priming effects on human umbilical cord blood-derived cultured mast cells; a higher dose, 100 ng/ml, was necessary for this priming. Conclusion: These results indicate that subthreshold doses of CRA-1 mAb or allergens can prime basophils and induce exaggerated responses to various IgE-independent stimuli. This may be a potentially important mechanism that explains environmental allergen-induced exacerbation of IgE-mediated allergic diseases such as asthma. Copyright © 2013 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
30. Eosinophil Degranulation during Pregnancy and after Delivery by Cesarean Section.
- Author
-
Matsumoto, Kenji, Ogasawara, Takahisa, Kato, Atsushi, Homma, Toshiki, Iida, Makoto, Akasawa, Akira, Wakiguchi, Hiroshi, and Saito, Hirohisa
- Subjects
- *
EOSINOPHILS , *MAST cells , *CONNECTIVE tissue cells , *NEUROTOXIC agents , *CESAREAN section - Abstract
Background: The physiological roles of eosinophils that accumulate in the uterus during pregnancy and of uterus-dwelling mast cells remain unknown. In the present study, we investigated the degranulation of eosinophils and mast cells within the normal course of pregnancy and after delivery by measuring the urinary concentrations of eosinophil-derived neurotoxin (EDN) and N-methylhistamine. Methods: Spot urine samples from 65 pregnant women and 15 nonpregnant, age-matched women were examined. Urinary EDN and N-methylhistamine concentrations were measured by radioimmunoassay and standardized with urinary creatinine concentration. Results: A significant increase in the urinary EDN concentration was observed until the second trimester in the normal pregnancies. The elevated urinary EDN levels decreased after the onset of labor in the third trimester and normalized within 1 month after normal vaginal delivery. In women who underwent a cesarean section, the urinary EDN concentration was significantly higher for up to 1 month after delivery, compared to that in women who underwent a vaginal delivery. In contrast, the urinary N-methylhistamine concentration did not change until the second trimester and was significantly decreased during the third trimester. No significant correlation between the peripheral blood eosinophil count and the urinary EDN concentration was observed in these subjects. Conclusions: Eosinophils appear to play a role in the progression of pregnancy and recovery after a cesarean section through the degranulation of eosinophils. In addition, mast cell degranulation does not appear to be related to the contraction of uterine smooth muscle during labor.Copyright © 2003 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
31. The Role of Eosinophils in Asthma: Sarastro or the Queen of the Night?
- Author
-
Matsumoto, Kenji and Saito, Hirohisa
- Subjects
- *
EOSINOPHILS , *ASTHMA , *MAST cells , *NUCLEIC acid probes , *AIRWAY (Anatomy) - Abstract
Eosinophils used to be thought of as regulators of allergic inflammation, but there is now evidence to the contrary; eosinophils have been found to be the major effector cells responsible for the late asthmatic response (LAR), airway hyperresponsiveness (AHR) and, at least in part, airway remodeling by releasing leukotrienes and highly basic and cytotoxic proteins such as major basic protein (MBP). However, a recent clinical trial using humanized anti-interleukin-5 monoclonal antibody found a failure to reduce AHR and the LAR, whereas the antibody entirely abolished tissue eosinophilia. In addition, abundant MBP has recently been found in mast cells as well as in eosinophils by our transcriptome (the whole transcripts that a cell expresses) screening of all leukocyte types. Eosinophils are indeed unlikely to be involved in the LAR and may not be unique cytotoxic cells in asthma. It is now necessary to determine the real role of eosinophils and whether early intervention to block eosinophil recruitment into the asthmatic lung does prevent airway remodeling and AHR.Copyright © 2001 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
32. Human Mast Cell Transcriptome Project.
- Author
-
Saito, Hirohisa, Nakajima, Toshiharu, and Matsumoto, Kenji
- Subjects
GENOMES ,MAST cells ,NUCLEIC acid probes ,GENES ,RNA - Abstract
After draft reading of the human genome sequence, systemic analysis of the transcriptome (the whole transcripts present in a cell) is progressing especially in commonly available cell types. Until recently, human mast cells were not commonly available. We have succeeded to generate a substantial number of human mast cells from umbilical cord blood and from adult peripheral blood progenitors. Then, we have examined messenger RNA selectively transcribed in these mast cells using high-density oligonucleotide probe arrays. Many unexpected but important transcripts were selectively expressed in human mast cells. We discuss the results obtained from transcriptome screening by introducing our data regarding mast-cell-specific genes.Copyright © 2001 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
33. Altered Expression of CD11b and CD62L after Cross-Linking of CD45 Isoforms on Human Eosinophils.
- Author
-
Matsumoto, Kenji, Bochner, Bruce, Wakiguchi, Hiroshi, and Kurashige, Takanobu
- Subjects
- *
EOSINOPHILS , *PHOSPHATASES , *MAST cells , *GRANULOCYTES , *LEUCOCYTES , *ESTERASES - Abstract
Background: Phosphotyrosine phosphatase CD45 is exclusively found on nucleated hematopoietic cells. CD45 is an essential component of signaling of mast cel degranulation and of lymphocyte activation, but little is known about its role in eosinophils. In the present study, we have determined the expression and function of CD45 isoforms on human eosinophils. Methods: Expression of CD45 isoforms on purified peripheral blood eosinophils was examined using indirect immunofluorescence and flow cytometry. Eosinophils were cultured with anti-CD45 isoform monoclonal antibodies (mAbs) for up to 24 h. Eosinophil activation was evaluated by measuring surface expression of CD11b or CD62L by flow cytometry. Results: Fresh eosinophils express CD45, CD45RB, CD45RO epitopes but not CD45RA. Incubation with anti-CD45 isoform mAb for 30 min did not alter the expression of either CD11b or CD62L on eosinophils. In contrast, the expression of CD11b was significantly enhanced after 24 h of incubation with mAbs against CD45, CD45RB, or CD45RO. In addition, the expression of CD62L was also significantly reduced with anti-CD45RB and with anti-CD45RO mAbs. Conclusions: Cross-linking of surface CD45 isoforms for 24 h significantly induced upregulation of CD11b expression and CD62L shedding, consistent with activation of eosinophils. Our data suggest that CD45 isoforms are functionally expressed on human eosinophils, and are capable of modulating eosinophil function and might participate in allergic inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 1998
- Full Text
- View/download PDF
34. Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation.
- Author
-
Ando, Tomoaki, Matsumoto, Kenji, Namiranian, Siavash, Yamashita, Hirotaka, Glatthorn, Haley, Kimura, Miho, Dolan, Brandon R, Lee, James J, Galli, Stephen J, Kawakami, Yuko, Jamora, Colin, and Kawakami, Toshiaki
- Subjects
- *
MAST cells , *ALLERGENS , *SKIN inflammation - Abstract
A correction to the article "Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation" that was published in the September 11, 2014 issue is presented.
- Published
- 2015
- Full Text
- View/download PDF
35. Histamine-releasing factor has a proinflammatory role in mouse models of asthma and allergy.
- Author
-
Kashiwakura, Jun-ichi, Ando, Tomoaki, Matsumoto, Kenji, Kimura, Miho, Kitaura, Jiro, Matho, Michael H., Zajonc, Dirk M., Ozeki, Tomomitsu, Ra, Chisei, MacDonald, Susan M., Siraganian, Reuben P., Broide, David H., Kawakami, Yuko, and Kawakami, Toshiaki
- Subjects
- *
MAST cells , *BASOPHILS , *ASTHMA , *HISTAMINE , *TUMOR proteins - Abstract
IgE-mediated activation of mast cells and basophils underlies allergic diseases such as asthma. Histamine-releasing factor (HRF; also known as translationally controlled tumor protein [TCTP] and fortilin) has been implicated in late-phase allergic reactions (LPRs) and chronic allergic inflammation, but its functions during asthma are not well understood. Here, we identified a subset of IgE and IgG antibodies as HRF-interacting molecules in vitro. HRF was able to dimerize and bind to Igs via interactions of its N-terminal and internal regions with the Fab region of Igs. Therefore, HRF together with HRF-reactive IgE was able to activate mast cells in vitro. In mouse models of asthma and allergy, Ig-interacting HRF peptides that were shown to block HRF/Ig interactions in vitro inhibited IgE/HRF-induced mast cell activation and in vivo cutaneous anaphylaxis and airway inflammation. Intranasally administered HRF recruited inflammatory immune cells to the lung in naive mice in a mast cell-- and Fc receptor--dependent manner. These results indicate that HRF has a proinflammatory role in asthma and skin immediate hypersensitivity, leading us to suggest HRF as a potential therapeutic target. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
36. TSLP is a negative regulator of RANKL-induced osteoclastogenesis.
- Author
-
Ohno, Tatsukuni, Nakamura, Takashi, Nakae, Susumu, Morita, Hideaki, Matsumoto, Kenji, Saito, Hirohisa, Takeda, Kazuyoshi, Okumura, Ko, and Azuma, Toshifumi
- Subjects
- *
THYMIC stromal lymphopoietin , *INNATE lymphoid cells , *TH2 cells , *MAST cells , *TRANCE protein , *OSTEOCLASTOGENESIS - Abstract
Thymic stromal lymphopoietin (TSLP) is a member of the IL-2 cytokine family, which is known to activate type 2 innate lymphoid cells, mast cells, and Th2 cells; this activation results in allergic inflammation and host defense against parasites. TSLP has also been shown to promote Th17-mediated immune responses, such as those observed in the development of rheumatoid arthritis; however, its role in osteoclastogenesis remains poorly understood. Here, we investigated the functional involvement of TSLP in RANKL-induced osteoclast differentiation from murine bone marrow-derived macrophages (BMMs). Both RANK− and RANK+ macrophages expressed TSLP receptor (TSLPR), while RANK+ osteoclast precursors maintained TSLPR expression after RANKL stimulation. TSLP stimulation led to inhibition of RANK-induced osteoclast differentiation in wild-type BMMs, but not Tslpr −/− BMMs; TSLP stimulation also led to suppression of osteoclastogenic gene expression (Nfatc1 , Acp5 , Mmp9 , and Ctsk). These inhibitory effects of TSLP were significantly reduced following STAT1 inhibition. Finally, we found that LPS stimulation induced TSLP production in murine calvarial osteoblasts, but not BMMs. Together, these observations suggest that TSLP acts directly on osteoclast precursors to suppress osteoclastogenesis. Osteoblasts, along with other TSLP-producing cells, may therefore contribute to the inhibition of osteoclastogenesis under inflammatory conditions. • RANK+ macrophages express TSLP receptor. • TSLP inhibits RANKL-induced osteoclast differentiation. • STAT1 inhibition diminishes inhibitory effects of TSLP in osteoclast differentiation. • LPS-stimulated osteoblasts produce TSLP. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
37. Role of Interleukin-33 in Innate-Type Immune Cells in Allergy.
- Author
-
Nakae, Susumu, Morita, Hideaki, Ohno, Tatsukuni, Arae, Ken, Matsumoto, Kenji, and Saito, Hirohisa
- Subjects
- *
INTERLEUKIN-33 , *ALLERGIES , *PATHOLOGY , *EPITHELIAL cells , *MAST cells - Abstract
lnterleukin-33 (IL-33), a member of the IL-1 cytokine family, is preferentially and constitutively expressed in epithelial cells, and it is especially localized in the cells' nucleus. The nuclear IL-33 is released by necrotic cells after tissue injury and/or trauma, and subsequently provokes local inflammation as an alarmin, like high-mobility group box protein-1 (HMGB-1) and IL-1α. IL-33 mainly activates Th2 cells and such innate-type immune cells as mast cells, basophils, eosinophils and natural helper cells that express IL-33R (a heterodimer of IL-1 receptor-like 1 [IL-1RL1; also called ST, T, Der4, fit-1] and IL-1 receptor accessory protein [IL-1RAcP]). That activation causes the cells to produce Th2 cytokines, which contribute to host defense against nematodes. On the other hand, excessive and/or inappropriate production of IL-33 is also considered to be involved in the development of such disorders as allergy. In this review, we summarize current knowledge regarding the pathogenic roles of IL-33 in the development of allergic inflammation by focusing on its effects on innate-type immune cells. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.