1. Isolation and characterization of a glycosyltransferase with specific catalytic activity towards flavonoids from Tripterygium wilfordii.
- Author
-
Lu, Yun, Ma, Bao-Wei, Gao, Jie, Tu, Li-Chan, Hu, Tian-Yuan, Zhou, Jia-Wei, Liu, Yuan, Tu, Yu-He, Lin, Zhao-Shou, Huang, Lu-Qi, and Gao, Wei
- Subjects
- *
BIOLOGICAL products , *FLAVONOIDS , *LIQUID chromatography , *MASS spectrometry , *MOLECULAR structure , *QUERCETIN , *TRANSFERASES , *PLANT extracts - Abstract
Flavonoids are important secondary metabolites that exist in many medicinal plants. Flavonoid glycosyltransferases can transfer sugar moieties to their parent rings, producing various flavonoid glycosides with significant pharmacological activities. Here, we report the molecular cloning of the O-glycosyltransferase TwUGT2 from Tripterygium wilfordii and its catalytic activity was explored by heterologous expression in E. coli. The results showed that TwUGT2 has specific glycosyltransferase activity towards C-3 and 7 hydroxyl groups of flavonoids, thereby converting quercetin and pinocembrin into isoquercitrin and pinocembrin 7-O-beta-D-glucoside, respectively. The identification of TwUGT2 will provide a useful molecular tool for synthetic biology and contribute to drug discovery. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF