18 results on '"Leize-Wagner, Emmanuelle"'
Search Results
2. Intact monoclonal antibodies separation and analysis by sheathless capillary electrophoresis-mass spectrometry.
- Author
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Giorgetti J, Lechner A, Del Nero E, Beck A, François YN, and Leize-Wagner E
- Subjects
- Glycosylation, Isomerism, Protein Processing, Post-Translational, Trastuzumab isolation & purification, Electrophoresis, Capillary methods, Mass Spectrometry methods, Trastuzumab chemistry
- Abstract
Capillary electrophoresis-mass spectrometry coupling is a growing technique in biopharmaceutics characterization. Assessment of monoclonal antibodies is well known at middle-up and bottom-up levels to obtain information about the sequence, post-translational modifications and degradation products. Intact protein analysis is an actual challenge to be closer to the real protein structure. At this level, actual techniques are time consuming or cumbersome processes. In this work, a 20 minutes separation method has been developed to optimize characterization of intact monoclonal antibodies. Thus, separation has been done on a positively charged coated capillary with optimized volatile background electrolyte and sample buffer. Three world-wide health authorities approved monoclonal antibodies have been used to set up a rapid and ease of use method. Intact trastuzumab, rituximab and palivizumab isoforms have been partially separated with this method in less than 20 minutes under denaturing conditions. For each monoclonal antibody, 2X-glycosylated and 1X-glycosylated structures have been identified and separated. Concerning basic and acidic variants, potential aspartic acid isomerization modification and asparagine deamidation have been observed. Accurate mass determination for high-mass molecular species remains a challenge, but the progress in intact monoclonal antibodies separation appears very promising for biopharmaceutics characterization.
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- 2019
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3. Monitoring of the retinoic acid receptor-retinoid X receptor dimerization upon DNA binding by native mass spectrometry.
- Author
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Nguyen-Huynh NT, Osz J, Peluso-Iltis C, Rochel N, Potier N, and Leize-Wagner E
- Subjects
- Dimerization, Models, Molecular, DNA metabolism, Mass Spectrometry methods, Receptors, Retinoic Acid metabolism, Retinoid X Receptors metabolism
- Abstract
Identifying protein-DNA interactions is essential to understand the regulatory networks of cells and their influence on gene expression. In this study, we use native electrospray mass spectrometry (ESI-MS) to investigate how the heterodimerization of retinoic acid receptor-retinoid X receptor (RAR-RXR) is mediated by DNA sequence. In presence of various RAR response elements (RAREs), three oligomeric states of RAR-RXR DNA binding domains (DBDs) bound to RAREs (monomer, homo- or heterodimers) were detected and individually monitored to follow subunit assembly and disassembly upon RAREs' abundancy or sequence. In particular, a cooperative heterodimerization was shown with RARb2 DR5 (5 base pair spaced direct repeat) while a high heterogeneity reflecting random complex formation could be observed with the DR0 response elements, in agreement with native gel electrophoresis data or molecular modeling. Such MS information will help to identify the composition of species formed in solution and to define which DR sequence is specific for RAR-RXR heterodimerization., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
4. Characterization of cetuximab Fc/2 dimers by off-line CZE-MS.
- Author
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François YN, Biacchi M, Said N, Renard C, Beck A, Gahoual R, and Leize-Wagner E
- Subjects
- Dimerization, Cetuximab chemistry, Electrophoresis, Capillary methods, Mass Spectrometry methods
- Abstract
Monoclonal antibody (mAb) therapeutics attract the largest concern due to their strong therapeutic potency and specificity. The Fc region of mAbs is common to many new biotherapeutics as biosimilar, antibody drug conjugate or fusion protein. Fc region has consequences for Fc-mediated effector functions that might be desirable for therapeutic applications. As a consequence, there is a continuous need for improvement of analytical methods to enable fast and accurate characterization of biotherapeutics. Capillary zone electrophoresis-Mass spectrometry couplings (CZE-MS) appear really attractive methods for the characterization of biological samples. In this report, we used CZE-MS systems developed in house and native MS infusion to allow precise middle-up characterization of Fc/2 variant of cetuximab. Molecular weights were measured for three Fc/2 charge variants detected in the CZE separation of cetuximab subunits. Two Fc/2 C-terminal lysine variants were identified and separated. As the aim is to understand the presence of three peaks in the CZE separation for two Fc/2 subunits, we developed a strategy using CZE-UV/MALDI-MS and CZE-UV/ESI-MS to evaluate the role of N-glycosylation and C-terminal lysine truncation on the CZE separation. The chemical structure of N-glycosylation expressed on the Fc region of cetuximab does not influence CZE separation while C-terminal lysine is significantly influencing separation. In addition, native MS infusion demonstrated the characterization of Fc/2 dimers at pH 5.7 and 6.8 and the first separation of these dimers using CZE-MS., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
5. Improved mass spectrometry compatibility is afforded by ammoniacal silver staining.
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Chevallet M, Diemer H, Luche S, van Dorsselaer A, Rabilloud T, and Leize-Wagner E
- Subjects
- Electrophoresis, Gel, Two-Dimensional, Humans, Isoelectric Focusing, Peptides chemistry, Reproducibility of Results, Ammonia chemistry, Mass Spectrometry methods, Proteomics instrumentation, Proteomics methods, Silver Staining methods
- Abstract
Sequence coverage in MS analysis of protein digestion-derived peptides is a key issue for detailed characterization of proteins or identification at low quantities. In gel-based proteomics studies, the sequence coverage greatly depends on the protein detection method. It is shown here that ammoniacal silver detection methods offer improved sequence coverage over standard silver nitrate methods, while keeping the high sensitivity of silver staining. With the development of 2D-PAGE-based proteomics, another burden is placed on the detection methods used for protein detection on 2-D-gels. Besides the classical requirements of linearity, sensitivity, and homogeneity from one protein to another, detection methods must now take into account another aspect, namely their compatibility with MS. This compatibility is evidenced by two different and complementary aspects, which are (i) the absence of adducts and artefactual modifications on the peptides obtained after protease digestion of a protein detected and digested in - gel, and (ii) the quantitative yield of peptides recovered after digestion and analyzed by the mass spectrometer. While this quantitative yield is not very important per se, it is however a crucial parameter as it strongly influences the S/N of the mass spectrum and thus the number of peptides that can be detected from a given protein input, especially at low protein amounts. This influences in turn the sequence coverage and thus the detail of the analysis provided by the mass spectrometer.
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- 2006
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6. Novel sheathless CE-MS interface as an original and powerful infusion platform for nanoESI study: from intact proteins to high molecular mass noncovalent complexes
- Author
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Gahoual, Rabah, Busnel, Jean-Marc, Wolff, Philippe, François, Yannis Nicolas, and Leize-Wagner, Emmanuelle
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- 2014
- Full Text
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7. Revealing the potential of capillary electrophoresis/mass spectrometry: the tipping point.
- Author
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Gahoual, Rabah, Leize‐Wagner, Emmanuelle, Houzé, Pascal, and François, Yannis‐Nicolas
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ELECTROSPRAY ionization mass spectrometry , *CAPILLARY electrophoresis , *MASS spectrometry , *BIOMOLECULES - Abstract
The hyphenation of capillary electrophoresis and mass spectrometry (CE/MS) remains a minor technique compared with liquid chromatography/mass spectrometry (LC/MS), which represents nowadays the standard instrumentation, regardless of its introduction thirty years ago. However, from a theoretical point of view, CE coupling should be quite favorable especially with electrospray ionization mass spectrometry (ESI‐MS). At the time, the sensitivity provided by CE/MS was often limited, due to hyphenation requirements, which at some point appeared to disqualify CE/MS from benefiting from the performance gain driving the evolution of MS instruments. However, this context has been significantly modified in a matter of a few years. The development of innovative CE/MS interfacing systems has enabled an important improvement regarding sensitivity and reinforced robustness in order to provide an instrumentation accessible to the largest scientific community. Because of the unique selectivity delivered by the electrophoretic separation, CE/MS has proved to be particularly relevant for the analysis of biological molecules. The conjunction of these aspects is motivating the interest in CE/MS analysis and shows that CE/MS is mature enough to enrich the toolbox of analytical techniques for the analysis of complex biological samples. Here we discuss the characteristics of the major types of high‐sensitivity CE/ESI‐MS instrumentation and emphasize the late evolution and future positioning of CE/MS analysis for the characterization of biological molecules like peptides and proteins, through some pertinent applications. [ABSTRACT FROM AUTHOR]
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- 2019
- Full Text
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8. Power and limitations of electrophoretic separations in proteomics strategies
- Author
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Rabilloud, Thierry, Vaezzadeh, Ali R, Potier, Noelle, Lelong, Cécile, Leize-Wagner, Emmanuelle, Chevallet, Mireille, Biochimie et biophysique des systèmes intégrés (BBSI), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire de Protéomique Clinique, Service de Médecine de Laboratoire, HCUG, Hôpital Universitaire de Genève, Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), and Hôpital Universitaire de Genève = University Hospitals of Geneva (HUG)
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Genomics (q-bio.GN) ,Electrophoresis ,Proteomics ,MESH: Mass Spectrometry ,MESH: Humans ,Proteome ,MESH: Peptides ,MESH: Proteomics ,MESH: Research Design ,Proteins ,MESH: Electrophoresis ,Mass Spectrometry ,MESH: Proteome ,Research Design ,FOS: Biological sciences ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Animals ,Humans ,Quantitative Biology - Genomics ,MESH: Animals ,MESH: Proteins ,Peptides - Abstract
International audience; Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis. In this review, the electrophoretic techniques are under scrutiny. Their principles are recalled, and their applications for peptide and protein separations are presented and critically discussed. In addition, the features that are specific to gel electrophoresis and that interplay with mass spectrometry (i.e., protein detection after electrophoresis, and the process leading from a gel piece to a solution of peptides) are also discussed.
- Published
- 2009
9. Advanced Antibody-Drug Conjugate Structural Characterization by Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry Using Complementary Approaches.
- Author
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Said, Nassur, Giorgetti, Jérémie, Kuhn, Lauriane, Beck, Alain, Leize-Wagner, Emmanuelle, Gahoual, Rabah, and Francois, Yannis-Nicolas
- Subjects
ANTIBODY-drug conjugates ,THERAPEUTIC use of monoclonal antibodies ,CAPILLARY electrophoresis ,IONIZATION (Atomic physics) ,MASS spectrometry - Abstract
Antibody-drug conjugates (ADCs) are an emerging category of biotherapeutic products based on monoclonal antibodies (mAbs) coupled to powerful cytotoxic drugs. The production of ADCs entails the formation of species with different number of conjugates drugs. The heterogeneity of ADC species add to the complexity originating from the mAb microvariability. Sheathless capillary electrophoresis- mass spectrometry (sheathless CE-MS) using complementary approaches was used to perform a detailed characterization of brentuximab vedotin. A sheathless CE-MS instrument used as a nanoelectrospray ionization (nanoESI) infusion platform was involved to perform the intact and middle-up analysis in native mass spectrometry (MS) conditions. The nanoESI infusion approaches enabled estimation of the average drug-to-antibody ratio (DAR) and the drug load distribution. A single injection with a sheathless capillary zone electrophoresis (CZE)-MS/MS method was sufficient to characterize the amino acid sequence with complete sequence coverage. In addition glycosylation and drug-loaded peptides could be identified from MS/MS spectra, which revealed robust information about their localizations and abundances. A drug-loaded peptide fragmentation mass spectra study yielded drug-specific fragments, which reinforced the confidence about the identifications. The results reveal the ability of the sheathless CZE-MS/MS method to characterize an ADCs primary structure in a single experiment. [ABSTRACT FROM AUTHOR]
- Published
- 2017
10. Improvement of Mitochondria Extract from Saccharomyces cerevisiae Characterization in Shotgun Proteomics Using Sheathless Capillary Electrophoresis Coupled to Tandem Mass Spectrometry.
- Author
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Ibrahim, Marianne, Gahoual, Rabah, Enkler, Ludovic, Becker, Hubert Dominique, Chicher, Johana, Hammann, Philippe, François, Yannis-Nicolas, Kuhn, Lauriane, and Leize-Wagner, Emmanuelle
- Subjects
MITOCHONDRIA ,SACCHAROMYCES ,PROTEOMICS ,CAPILLARY electrophoresis ,MASS spectrometry - Abstract
In this work, we describe the characterization of a quantity-limited sample (100 ng) of yeast mitochondria by shotgun bottom-up proteomics. Sample characterization was carried out by sheathless capillary electrophoresis, equipped with a high sensitivity porous tip and coupled to tandem mass spectrometry (CESI-MS-MS) and concomitantly with a state-of-art nano flow liquid chromatography coupled to a similar mass spectrometry (MS) system (nanoLC-MS-MS). With single injections, both nanoLC-MS-MS and CESI-MS-MS 60 min-long separation experiments allowed us to identify 271 proteins (976 unique peptides) and 300 proteins (1,765 unique peptides) respectively, demonstrating a significant specificity and complementarity in identification depending on the physicochemical separation employed. Such complementary, maximizing the number of analytes detected, presents a powerful tool to deepen a biological sample's proteomic characterization. A comprehensive study of the specificity provided by each separating techniquewas also performed using the different properties of the identified peptides: molecular weight, mass-to-charge ratio (m/z), isoelectric point (pI), sequence coverage or MS-MS spectral quality enabled to determine the contribution of each separation. For example, CESI-MS-MS enables to identify larger peptides and eases the detection of those having extreme pI without impairing spectral quality. The addition of peptides, and therefore proteins identified by both techniques allowed us to increase significantly the sequence coverages and then the confidence of characterization. In this study, we also demonstrated that the two yeast enolase isoenzymes were both characterized in the CESI-MS-MS data set. The observation of discriminant proteotypic peptides is facilitated when a high number of precursors with high-quality MS-MS spectra are generated. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
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11. Comprehensive Multilevel Characterization of Biologies Using Sheathless Capillary Electrophoresis Hyphenated to Tandem Mass Spectrometry.
- Author
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Gahoual, Rabah, Biacchi, Michaël, Busnel, Jean-Marc, Beck, Alain, François, Yannis-Nicolas, and Leize-Wagner, Emmanuelle
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CAPILLARY electrophoresis ,MASS spectrometry ,DEAMINATION ,POST-translational modification ,ASPARAGINE ,AMINO acid sequence ,RACEMIZATION - Abstract
An innovative coupling of capillary electrophoresis and tandem mass spectrometry (CE-MS-MS) provides excellent results for the characterization of the primary structure of biologies. Using a single 200-fmol injection, it is possible to completely characterize the amino acid sequence and obtain information about the location, structure, and relative abundance of 15 glycoforms. Finally, various post-translational modifications can be characterized and their relative abundances estimated, including asparagine deamidation and aspartic acid racemization. And in a biosimilarity assessment between two monoclonal antibodies, it was possible to distinguish minor differences such as a sole amino acid substitution. This level of characterization is permitted by cumulating the specificities of CE and high-resolution tandem MS using a sheathless interface. [ABSTRACT FROM AUTHOR]
- Published
- 2015
12. Cutting-edge capillary electrophoresis characterization of monoclonal antibodies and related products.
- Author
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Gahoual, Rabah, Beck, Alain, Leize-Wagner, Emmanuelle, and François, Yannis-Nicolas
- Subjects
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CAPILLARY electrophoresis , *THERAPEUTIC use of monoclonal antibodies , *ANTIBODY-drug conjugates , *CHIMERIC proteins , *DRUG synergism , *ANTIBODY specificity , *BIOPHARMACEUTICS - Abstract
Out of all categories, monoclonal antibodies (mAbs), biosimilar, antibody-drug conjugates (ADCs) and Fc-fusion proteins attract the most interest due to their strong therapeutic potency and specificity. Because of their intrinsic complexity due to a large number of micro-heterogeneities, there is a crucial need of analytical methods to provide comprehensive in-depth characterization of these molecules. CE presents some obvious benefits as high resolution separation and miniaturized format to be widely applied to the analysis of biopharmaceuticals. CE is an effective method for the separation of proteins at different levels. capillary gel electrophoresis (CGE), capillary isoelectric focusing (cIEF) and capillary zone electrophoresis (CZE) have been particularly relevant for the characterization of size and charge variants of intact and reduced mAbs, while CE–MS appears to be a promising analytical tool to assess the primary structure of mAbs and related products. This review will be dedicated to detail the current and state-of-the-art CE-based methods for the characterization of mAbs and related products. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
13. Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – Mass spectrometry.
- Author
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Giorgetti, Jérémie, Beck, Alain, Leize-Wagner, Emmanuelle, and François, Yannis-Nicolas
- Subjects
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MONOCLONAL antibodies , *MASS spectrometry , *CAPILLARY electrophoresis , *DEAMINATION , *BIOPHARMACEUTICS , *PROTEOLYSIS - Abstract
• We implement a multi-level characterization of seven commecialized mAbs. • We provide structural characterization of mAbs as primary structure, N-glycoform and PTMs. • We confirm the relevance of CE-MS coupling for biotherapeutics characterization. Significant growth of biopharmaceuticals requires powerful analytical methods to better understand their structure by establishing a complete characterization. To this end, a combination of bottom-up, middle-up and intact molecule levels with a capillary electrophoresis-mass spectrometry coupling has been performed to have a comprehensive picture of monoclonal antibodies. In this study, 7 worldwide health authorities approved mAbs have been analyzed to get information about their charge heterogeneity, the identification of post translational modifications (PTMs), their location and relative quantitation. Intact mAbs isoforms have been partially separated in less than 12 min and enabled to have a global illustration of mAbs heterogeneity and high masses PTMs characterization notably major N-glycosylation forms. Particularly, 2X-glycosylated and 1X-glycosylated forms have been partially separated. To deepen characterize PTMs carried by the backbone structure, advanced investigations at a middle-up level have been performed. Limited IdeS proteolysis allowed to study independently Fc/2 and F(ab)'2 fragments. Following the same separation conditions, isoforms of these fragments have been separated and data interpretation allowed to disclose additional PTMs as K-clip, oxidations or deamidations. A second intermediate level has been examined by adding a reduction step to establish a more precise assessment of PTMs and isoforms from the F(ab)'2 fragment. This reduction step released the light chains from the Fd fragment to get only 25 kDa fragments to analyze. CE-ESI-MS coupling allowed to get more information particularly about low masses PTMs. The precise location and relative quantitation of each PTM has been investigated at the peptidic level induced by a tryptic digestion of the studied mAbs. The concordance of the results shows the efficiency of the CE-ESI-MS coupling to characterize mAbs and highlight the need of the multi-level combination to get a comprehensive characterization of biotherapeutics. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
14. Insights from capillary electrophoresis approaches for characterization of monoclonal antibodies and antibody drug conjugates in the period 2016–2018.
- Author
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Lechner, Antony, Giorgetti, Jérémie, Gahoual, Rabah, Beck, Alain, Leize-Wagner, Emmanuelle, and François, Yannis-Nicolas
- Subjects
- *
CAPILLARY electrophoresis , *MONOCLONAL antibodies , *ISOELECTRIC focusing , *THERAPEUTIC use of proteins , *CROSS-entropy method , *IMMUNOGLOBULINS - Abstract
Monoclonal antibodies (mAbs) and their related products as antibody-drug-conjugates (ADCs) or biosimilars represent a constantly growing class of molecules therapeutic proteins used as treatment against numerous diseases. These compounds can undergo several modifications which could alter the efficiency of treatments. In this context, several analytical methods were designed to deliver a comprehensive structural characterization and guarantee the quality of biotherapeutics. Capillary electrophoresis (CE) is considered today as a major technique for the analysis of biotherapeutics due to benefic characteristics as high resolution separation and miniaturized format. Different CE modes have been developed to characterize mAbs at different levels such as capillary gel electrophoresis (CGE), capillary isoelectric focusing (cIEF), and capillary zone electrophoresis (CZE). Recent developments in CE-mass spectrometry (MS) coupling assessed this technology as a promising tool to obtain high level structural characterization of biopharmaceuticals. Moreover, upcoming techniques such as 2D CE-MS and microfluidic systems are now emerging to offer new possibilities beyond actual limits. This review will be dedicated to discuss the state-of-the-art CE-based methods for the characterization of mAbs and ADCs in the period 2016–2018. • Complete update of CE-based methods as CE-SDS, cIEF, icIEF and CZE for the characterization of mAbs and related product. • Relevance of CE as a reference method for mAbs and related product characterization. • Last developments of CE-MS coupling for mAbs and ADCs characterization. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
15. Method development for the LC-MS/MS determination of C60 and C70 fullerenes and their functionalized derivatives in airborne particulate matter, settled dust and soot.
- Author
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Galmiche, Mathieu, Esslinger, Eden, Delhomme, Olivier, Schaeffer, Philippe, Motsch, Estelle, Leize-Wagner, Emmanuelle, François, Yannis-Nicolas, and Millet, Maurice
- Subjects
- *
PARTICULATE matter , *FULLERENE derivatives , *LIQUID chromatography-mass spectrometry , *DUST , *SOOT , *CHEMICAL structure , *SOLVENT extraction , *LIQUID chromatography - Abstract
Fullerenes are organic contaminants of growing concern due to their increasing use for industrial purposes, in addition to their emission by natural and anthropogenic combustion sources. Their analysis is made tricky by their unique chemical structure and their acute hydrophobicity. Thus, analytical developments are required for further environmental evaluation of their occurrence. This article presents such a development for the analysis of C 60 and C 70 fullerenes together with three of their functionalized derivatives in airborne particulate matter, settled dust and soot from diesel exhaust. Pressurized Liquid Extraction was performed with toluene and acetonitrile as extraction solvents, and gave mean extraction recoveries from 75% to 95% for the five fullerenes investigated. Liquid Chromatography was optimized in terms of stationary phase and mobile phase to obtain a good separation of the compounds. Optimal separation of the compounds by liquid chromatography were obtained using a C18 stationary phase and a mixture of toluene - acetonitrile as mobile phase in gradient mode. The mass spectrometry detection method was also optimized with a comparison of three ionization sources: Electrospray ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI) and Atmospheric Pressure PhotoIonization (APPI). APPI gave the most sensitive and repeatable detection with instrumental limits of quantification (LOQ) down to 10 pg injected. MS/MS was used in pseudo-MRM mode to enhance signal intensity. The optimized LC-APPI-MS/MS method was then validated and applied to indoor PM 10 and household dust samples, and to a Diesel Particulate Matter Reference Material. None of these samples showed concentrations of fullerenes above the method quantification limits. [Display omitted] • A LC-MS/MS method was developed for the analysis of C 60 , C 70 and three derivatives. • APPI gave the best sensitivity and method performance as compared to APCI and ESI. • Instrumental LOQ were from 10 pg for N -methylfulleropyrrolidine to 100 pg for C 60. • All mean method recoveries were between 75% and 95% thanks to PLE. • No fullerene was quantified above the LOQ in PM 10 and settled dust samples. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
16. Omics for Precious Rare Biosamples: Characterization of Ancient Human Hair by a Proteomic Approach.
- Author
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Fresnais, Margaux, Richardin, Pascale, Sepúlveda, Marcela, Leize-Wagner, Emmanuelle, and Charrié-Duhaut, Armelle
- Subjects
- *
LIQUID chromatography-mass spectrometry , *ARCHAEOLOGY , *KERATIN , *PROTEOMICS , *ECOLOGICAL experiments , *BIOLOGICAL adaptation - Abstract
Omics technologies have far-reaching applications beyond clinical medicine. A case in point is the analysis of ancient hair samples. Indeed, hair is an important biological indicator that has become a material of choice in archeometry to study the ancient civilizations and their environment. Current characterization of ancient hair is based on elemental and structural analyses, but only few studies have focused on the molecular aspects of ancient hair proteins-keratins-and their conservation state. In such cases, applied extraction protocols require large amounts of raw hair, from 30 to 100 mg. In the present study, we report an optimized new proteomic approach to accurately identify archeological hair proteins, and assess their preservation state, while using a minimum of raw material. Testing and adaptation of three protocols and of nano liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) parameters were performed on modern hair. On the basis of mass spectrometry data quality, and of the required initial sample amount, the most promising workflow was selected and applied to an ancient archeological sample, dated to about 3880 years before present. Finally, and importantly, we were able to identify 11 ancient hair proteins and to visualize the preservation state of mummy's hair from only 500 μg of raw material. The results presented here pave the way for new insights into the understanding of hair protein alteration processes such as those due to aging and ecological exposures. This work could enable omics scientists to apply a proteomic approach to precious and rare samples, not only in the context of archeometrical studies but also for future applications that would require the use of very small amounts of sample. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
17. Top-down and middle-down approach by fraction collection enrichment using off-line capillary electrophoresis – mass spectrometry coupling: Application to monoclonal antibody Fc/2 charge variants.
- Author
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Biacchi, Michael, Said, Nassur, Beck, Alain, Leize-Wagner, Emmanuelle, and François, Yannis-Nicolas
- Subjects
- *
CAPILLARY electrophoresis , *MASS spectrometry , *MONOCLONAL antibodies , *CETUXIMAB , *FRAGMENTATION reactions - Abstract
The characterization of complex protein mixtures represents one of the biggest challenge in many research fields such as biological or biopharmaceutical sciences. Out of all categories, monoclonal antibodies (mAbs) and related products drawn the most interest due to their strong therapeutic potency and specificity. Because of their intrinsic complexity due to a large number of micro-heterogeneities, there is a crucial need for analytical methods to provide comprehensive in-depth characterization of these proteins. In this work, we developed a methodology using CE-UV/MALDI-MS to perform top-down or middle-down characterization after fraction collection enrichment applied to intact protein and mAbs samples. The performance of the method was evaluated with the rapid separation of three intact protein mixture. Good robustness of CZE separation and quality of MALDI-MS spectra and MALDI-ISD spectra of each protein confirms the usefulness of sample enrichment to obtain adequate quantity of deposed protein for top-down analysis and the proof of principle of the method. In a second step, the method was applied to the middle-down characterization of Fc/2 cetuximab variants. Identification of around 9% sequence coverage of Fc/2 cetuximab fragments allows to conclude on the feasibility of the strategy for middle-down characterization of Fc/2 cetuximab variants using CE-UV/MALDI-MS. Moreover, MALDI-ISD fragmentation of Fc/2 cetuximab variants confirm separation phenomenon based on the formation of Fc/2 dimers with and without C-terminal truncation. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
18. Identification of genes and proteins involved in the pleiotropic response to arsenic stress in Caenibacter arsenoxydans, a metalloresistant beta-proteobacterium with an unsequenced genome
- Author
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Carapito, Christine, Muller, Daniel, Turlin, Evelyne, Koechler, Sandrine, Danchin, Antoine, Van Dorsselaer, Alain, Leize-Wagner, Emmanuelle, Bertin, Philippe N., and Lett, Marie-Claire
- Subjects
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ARSENATES , *LIQUID chromatography , *CHROMATOGRAPHIC analysis , *MASS spectrometry - Abstract
Abstract: The effect of high concentrations of arsenic has been investigated in Caenibacter arsenoxydans, a β-proteobacterium isolated from an arsenic contaminated environment and able to oxidize arsenite to arsenate. As the genome of this bacterium has not yet been sequenced, the use of a specific proteomic approach based on nano-high performance liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) studies and de novo sequencing to perform cross-species protein identifications was necessary. In addition, a random mutational analysis was performed. Twenty-two proteins and 16 genes were shown to be differentially accumulated and expressed, respectively, in cells grown in the presence of arsenite. Two genes involved in arsenite oxidation and one in arsenite efflux as well as two proteins responsible for arsenate reduction were identified. Moreover, numerous genes and proteins belonging to various functional classes including information and regulation pathways, intermediary metabolism, cell envelope and cellular processes were also up- or down-regulated, which demonstrates that bacterial response to arsenic is pleiotropic. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
- View/download PDF
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