1. Direct and rapid quantitation of ephedrines in human urine by paper spray ionization/high resolution mass spectrometry.
- Author
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Jeong ES, Kim KH, Cha E, Kwon OS, Cha S, and Lee J
- Subjects
- Humans, Limit of Detection, Mass Spectrometry economics, Paper, Reproducibility of Results, Substance Abuse Detection economics, Central Nervous System Stimulants urine, Ephedrine analogs & derivatives, Ephedrine urine, Mass Spectrometry methods, Phenylpropanolamine urine, Pseudoephedrine urine, Substance Abuse Detection methods
- Abstract
A rapid and direct paper spray ionization/mass spectrometry (PSI/MS) method was developed for quantitative analysis of ephedrine, pseudoephedrine, norpseudoephedrine, and methylephedrine in human urine. This method involves the use of a triangular filter paper and high-resolution mass spectrometry, where the molecular ions of ephedrines were generated by applying high voltage after loading the spray solvent to the paper which urine sample was pre-loaded. Small amounts (2μL) of urine spiked with an internal standard were directly analyzed for ephedrines. The PSI/MS method was validated for linearity, within- and between-run precision, accuracy, and limit of detection. The results showed good linearity (R(2)≥0.9928) and acceptable precision and accuracy. Furthermore, the accuracy of the method was assessed by analyzing a blind urine sample from World Anti-Doping Agency and comparing the measured concentrations with the nominal concentrations. This test resulted in accuracies ranging from 96.4 to 106.1%, indicating that the PSI/MS method has the potential to be an alternative technique for the fast quantitation of ephedrines in doping control analysis., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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