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Objectives This study aimed to sequence the whole genome of Vibrio campbellii RT-1 strain. Methods V. campbellii strain was isolated from an infected shrimp, Litopenaeus vannamei collected from aquaculture ponds, India (12.1899° N, 79.9249° E). The whole genome sequencing (WGS) was performed using the Illumina Hiseq 2500 platform and assembled de novo using SPAdes and Velvet optimiser. Furthermore, the gene prediction and annotation were performed by a rapid prokaryotic genome tool-Prokka. Results The genome of V. campbellii RT-1 strain has one circular chromosome with 6327218 bp long. V. campbellii RT-1 strain contains 5787 predicted genes with an average of 45% GC content. A total of 86 known genes associated with pathogenicity were identified and 28 genes were found to be responsible for virulence factors. Furthermore, 1112 unigenes were subjected to Gene Ontology (GO) terms, and 4895 predicted proteins were annotated with Clusters of orthologous (COGs) functional groups. Conclusions The phylogenetic position of V. campbellii RT-1 strain was established through whole genome sequencing and genomic tools which provides a strong platform to further study on genomic alterations and phenotype of V. campbellii . [ABSTRACT FROM AUTHOR]

Highlights: [•] We investigated the effect of biogenic silver nanoparticles against marine biofilm forming bacteria. [•] Aspects such as formation of circular zone, quantification of biofilm formation and bacterial growth were assessed in the presence and absence of silver nanoparticles. [•] Marine biofilm bacteria were found to be controlled in the presence of silver nanoparticles. [ABSTRACT FROM AUTHOR]