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Objective: Ischemia is characterized by an inadequate supply of nutrients and oxygen due to reduced blood circulation to specific tissues or organs. Reperfusion injury refers to the detrimental effects of abrupt exposure of the ischemic tissue to elevated oxygen levels and subsequent generation of reactive oxygen derivatives inside the tissue. In surgical procedures characterized by frequent occurrences of ischemia-reperfusion (I/R), such as transplant and cardiovascular surgery, it is crucial to prioritize the preservation of tissue integrity and mitigation of associated damage. In this study, we formulated a hypothesis suggesting that carvacrol (Car), an aromatic hydrocarbon present in some plant species, might decrease I/R injury by implication of its antioxidant, anti-apoptotic, and anti-inflammatory functions. Methods: We planned our study with 18 rats, randomly divided into three groups: control group, I/R group, and I/R + Car group. In the control group, laparotomy was performed only, and blood and lower limb muscle tissue samples were taken. In the I/R group, after laparotomy, lower extremity ischemia is achieved for 60 min, and reperfusion is achieved for another 60 min. In I/R + Car, rats were administered 100 mg/kg Car via intraperitoneal injection 30 min after ischemia. Then, 60 min of ischemia and 60 min of reperfusion were achieved. Lower limb muscle samples were examined both histologically and biochemically. We evaluated the levels of malondialdehyde (MDA), glutathione-S transferase (GST), and catalase (CAT). In addition, serum ischemia-modified albumin (IMA) levels were measured. Results: Our study showed that the findings of I/R injury decreased prominently in the I/R + Car group's samples. GST, MDA, CAT, and IMA levels were significantly higher in the I/R group than in the control and I/R + Car groups. (p=0.024, p=0.010, p=0.030 and p<0.0001; respectively). Histopathological examination revealed no degeneration in the control group. Conversely, contraction, hypertrophy, nucleus degeneration, necrotic fibers, and hyalinization were observed in the I/R group. In the I/R + Car group, inflamed areas were less frequent than in the I/R group, and vascular dilatation of myofibre was noted. Conclusion: This study demonstrates that the Car molecule protects against I/R injury. Therefore, we contend that more experimental cohort investigations are needed to examine the impact of the Car molecule on preventing I/R injury. [ABSTRACT FROM AUTHOR]

Copyright of Turkish Journal of Trauma & Emergency Surgery / Ulusal Travma ve Acil Cerrahi Dergisi is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Etlik Veteriner Mikrobiyoloji Dergisi is the property of Veteriner Kontrol Merkez Arastirma Enstitusu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Turkish Journal of Trauma & Emergency Surgery / Ulusal Travma ve Acil Cerrahi Dergisi is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to evaluate the possible protective effect of ellagic acid (EA) on rats following renal ischemia-reperfusion (I/R) injury. Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 min without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 min of reperfusion. I/R+EA group were subjected to the same renal ischemia/reperfusion as the I/R group, were also given 85 mg/kg EA perorally 30 min prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues. I/R damage significantly increased serum MDA levels in the I/R group when compared with Sham group. Serum TAC level was significantly lower in I/R group than I/R+EA group. A significantly increase on OSI levels and decrease on TAC levels was found in the kidneys in I/R group. In I/R + EA group, EA reversed the negative effects of I/R injury. EA pretreatment was effective in decreasing tubular necrosis score. In conclusion; EA pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury. [ABSTRACT FROM AUTHOR]

Aim: To evaluate the protective effect of selenium on intestinal ischemia-reperfusion injury due to intussusception. Materials and methods: Forty Wistar albino rats were allocated to 4 equal groups (n = 10). After anesthetization, 2 cm of intestinal segment, 20 cm proximal to the ileocecal valve, was removed in the control group (CG). In the intussusception group (ING), intussusception was performed 20 cm proximal to the ileocecal valve by pushing the proximal intestinal segment distally with a stylet, and 2 cm of intestinal segment was removed 4 h later. Intussusception was reduced and a 2 cm segment was removed after another 4 h period in the intussusception-reduction group (IRG). The selenium group (SG) received 0.2 mg kg-1 of selenium in 10 mL of 0.9% NaCl solution intraperitoneally 20 min before reduction and segments were removed 4 h thereafter. Intestinal tissues were evaluated for histopathological grades and oxidative injury markers. Results: Histopathologic evaluations among the 4 groups did not reveal a significant difference (P > 0.05). Although catalase activity was decreased in all groups, increased activity was found in SG compared with IRG (P < 0.05). GSH-Px and SOD activities were also found to have decreased in ING and IRG (P < 0.05). CG and SG had no difference in GSH-Px and SOD activities (P > 0.05). Malondialdehyde (MDA) activities did not differ between CG and SG, but the MDA level was increased in ING and IRG when compared with CG (P < 0.05). Conclusion: Although selenium prevents oxidative injury by increasing the antioxidant enzyme activity in experimental intussusception, similar effects on histopathologic findings were not detected. [ABSTRACT FROM AUTHOR]