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We intended to identify the incidence and risk factors (RFs) for Postoperative urinary retention (POUR) after applying a risk-stratified catheterization optimization method in enhanced recovery after surgery (ERAS)-total joint arthroplasty (TJA).A total of 381 patients were prospectively monitored for POUR. POUR diagnosis was done by a perioperative specialist. Data on potential risk factors (RFs) for POUR were accumulated. Univariate analysis (UA) was conducted to identify possible indicators of POUR, followed by multivariate analysis (MA) of identified indicators.POUR occurred in 5.5% of cases, including 8 (3.4%) patients underwent total knee arthroplasty and 13 (10.4%) patients underwent total hip arthroplasty. In UA, age, sex, American Society of Anesthesiologists (ASA) score, and the type of operation were significantly different on UA (P = 0.046, P = 0.022, P = 0.000 and P = 0.049, respectively). Other additional predictors, including body mass index (BMI), international prostate symptom score (IPSS) score, preoperative haemoglobin (Hb), duration of operation, estimated intraoperative blood loss, intraoperative fluid volume, fluid infusion volume within 24 h postoperatively were not associated with POUR (P 0.05). MA results demonstrated that age, ASA score, type of operation and standard intraoperative placement of an indwelling bladder catheter (SIP-IBC) were strongly associated with POUR development risk (P 0.05).Overall, we had a low POUR incidence in our study cohort. However, with the shift from non-ERAS TJA to ERAS TJA protocol, it is crucial to closely monitor the male gender, advanced age, THA and SIP-IBC, as these variables can markedly enhance POUR risk.

CD147, also known as EMMPRIN or basigin, is a transmembrane glycoprotein receptor that activates matrix metalloproteinases and promotes inflammation. CD147 function is regulated by posttranslational modifications of which glycosylation has attracted the most attention. In this study, we demonstrated that glycosylated CD147 was the dominant form in heart tissue, and its levels were markedly elevated in response to transverse aortic constriction (TAC). Adeno-associated virus 9-mediated, cardiac-specific overexpression of wild-type CD147 in mice significantly promoted pressure overload-induced pathological cardiac remodeling accompanied by augmented oxidative stress and ferroptosis. By contrast, mutations of CD147 glycosylation sites notably weakened these detrimental effects of CD147. Mechanistically, CD147 exacerbated TAC-induced pathological cardiac remodeling via direct binding with the adaptor molecule TRAF2 and subsequent activation of TAK1 signalling, which was dependent on glycosylation of CD147. Collectively, our findings provide the first evidence that CD147 promoted pathological cardiac remodeling and dysfunction in a glycosylation-dependent manner through binding the adaptor protein TRAF2 and activating the downstream TRAF2-TAK1 signalling pathway. Thus, glycosylation of CD147 may be a potent interventional target for heart failure treatment.

Background Myeloid-derived suppressor cells (MDSCs) play immunosuppressive roles in cancers and some infectious diseases; however, their role in dengue fever (DF) remains unknown. This study evaluated the clinical significance of MDSCs in DF patients. Methods This study comprised 178 non-severe DF patients, 20 non-dengue fever (NDF) controls, and 30 healthy donors. The DF patients were divided into the following five groups based on the fever duration from its onset to the day of sample collection: fever duration of 1–2, 3–4, 5–6, 7–8, and > 9 days. Among these DF patients, 14 were monitored for eight days, and their peripheral blood samples were collected every two days. The mononuclear cells were isolated and analyzed using flow cytometry. The correlation between the MDSCs and clinical and immunological indicators of the DF patients was evaluated using Spearman analysis. Results The count of the peripheral blood MDSCs, especially monocytic MDSCs, of the 178 DF patients were dramatically higher than those of the NDF and healthy controls, and remarkably decreased with the fever duration. Moreover, the MDSC count correlated with some indicators, including the dengue viral load (rho = 0.367, p rho = 0.263, p = .005), prothrombin time (rho = 0.475, p + T cell number (rho = − 0.317, p + T cell number (rho = − 0.361, p rho = − 0.347, p rho = − 0.258, p = .001), interferon-α (IFN-α) (rho = 0.43, p rho = 0.278, p = .019). Furthermore, the level of arginase-1, but not nitric oxide, was higher in the DF patients than in the healthy controls and was closely related to the number of MDSCs (rho = 0.265, p = .024). Conclusions Our study reveals a significant correlation between MDSCs and DF clinical indicators, posing MDSCs as potential target cells for DF treatment.

OBJECTIVE: To study the clinical features and prognosis of preterm infants with varying degrees of bronchopulmonary dysplasia (BPD). METHODS: The clinical data of 144 preterm infants with a gestational age of < 32 weeks who were admitted to the neonatal intensive care unit from March 2014 to March 2016 and were diagnosed with BPD were collected. According to the severity of BPD, these preterm infants were divided into mild group with 81 infants and moderate/severe group with 63 infants. The two groups were compared in terms of perinatal risk factors, treatment, comorbidities, complications, and prognosis of the respiratory system. RESULTS: Compared with the mild BPD group, the moderate/severe BPD group had a significantly higher gestational age and rate of small-for-gestational-age (SGA) infants (P < 0.05), as well as a significantly higher rate of severe preeclampsia and a significantly lower rate of threatened preterm labor (P < 0.05). Compared with the mild BPD group, the moderate/severe BPD group had a significantly higher percentage of infants who needed mechanical ventilation at 2 weeks after birth, longer duration of mechanical ventilation, total time of oxygen therapy, and length of hospital stay, and higher incidence rates of pneumonia and cholestasis (P < 0.05), as well as a significantly lower application rate of caffeine citrate (P < 0.05). The multivariate logistic regression analysis showed that SGA birth (OR=5.974, P < 0.05), pneumonia (OR=2.590, P < 0.05), and mechanical ventilation required at 2 weeks after birth (OR=4.632, P < 0.05) were risk factors for increased severity of BPD. The pulmonary function test performed at the corrected gestational age of 40 weeks showed that compared with the mild BPD group, the moderate/severe BPD group had significantly lower ratio of time to peak tidal expiratory flow to total expiratory time, ratio of volume to peak tidal expiratory flow to total expiratory volume, and tidal expiratory flow at 25% remaining expiration (P < 0.05). The infants were followed up to the corrected gestational age of 1 year, and the moderate/severe BPD group had significantly higher incidence rates of recurrent hospital admission for pneumonia and recurrent wheezing (P < 0.05). CONCLUSIONS: SGA birth, pneumonia, and prolonged mechanical ventilation are associated with increased severity of BPD. Infants with moderate or severe BPD have poor pulmonary function and may experience recurrent infection and wheezing.

Young adult male Wistar rats, a species commonly used in inhalation toxicity studies, and OF1 mice, a species often used in sensory irritation studies, were simultaneously nose-only exposed for 45-min to ammonia in concentrations from 92 to 1243 mg/m 3 . This study examined airway reflexes by the changes in respiratory patterns elicited by ammonia in either dry, steam-humidified (approximately 95% relative humidity) or aqueous aerosol containing atmospheres. This served the objective to explore whether high concentrations of anhydrous ammonia and/or high humidity and aqueous aerosol change the predominant nasal deposition site to more distal locations in the lung. Animals from all groups tolerated the exposure without evidence of respiratory tract irritation, changes in body and lung weights. The evoked changes on breathing patterns resembled those known to occur following exposure to ‘ upper respiratory tract sensory irritants ’, rapid in onset and reversibility. Reflex stimulation from the lower airways was not observed in any group. While mice showed some adaption during the 45-min exposure period, rats displayed more stable changes in respiratory patterns. In this species humidity- or aqueous aerosol-related changes in sensory irritation potency did not occur to any appreciable extent. The respiratory decrease 50%, RD 50 , was 972 and 905 mg/m 3 in dry and wet air, respectively. In contrast, mice appeared to more susceptible to ammonia in presence of dry air (the RD 50 , was 582 and 732 mg/m 3 in dry and wet air, respectively). In summary, it was shown that the sensory irritation potency of ammonia does not increase when inhaling wet atmospheres nor penetrates this gas into the lower airways up to 1243 mg/m 3 × 45-min. The mild-to-moderate sensory irritation-related effects observed at ≈400 mg/m 3 (571 ppm) × 45-min do not appear to be offensive enough to impair escape as a result of upper airway sensations. Interestingly, this rat-based estimate matches almost exactly the experienced-based RAM TRAC recommendations of 696 and 492 ppm for 30 and 60 min, respectively.

To investigate the influence of electroacupuncture (EA) on lipid metabolism in both sexes of obesity model rats.A total of 30 male and 30 female SD rats were randomized into normal control, model and EA groups respectively, with 10 rats in each group. EA (2 Hz, 1-2 mA) was applied to the ipsilateral "Zusanli" (ST 36)-"Sanyin-jiao"(SP 6) and bilateral "Fenglong" (ST 40) for 30 min, once daily for 28 days. In addition,"Quchi" (LI 11),"Zhongwan" (CV 12) and "Guanyuan" (CV 4) were punctured with filiform needles and stimulated by manipulating the needle for 1 min, separately. Obesity model was duplicated by subcutaneous injection of 15% glutamic sodium (0.2 mL/10 g) once daily for 5 days and by feeding the animal with high fat forage for 36 days. Lee's index was calculated, and plasma triglyeride (TG) concentration detected by glycerol-phosphoric acid oxidase-peroxydase method, total cholesterol (TC) detected by cholesterol oxidase method, and plasma low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) contents were assayed by one-step method, respectively.In both male and female rats, compared to their own normal groups, the Lee's index, plasma TG, TC and LDL-C contents were increased significantly and plasma HDL-C contents decreased obviously in the model groups (P0.01), while compared to their own model groups, the Lee's index, plasma TG, TC and LDL-C levels in the EA groups were down-regulated considerably and plasma HDL-C contents up-regulated remarkably after the treatment (P0.01, P0.05). The efficacies of the EA intervention were obviously better in reducing Lee's index for male rats, and in down-regulating plasma TG and TC contents in female rats (P0.05). No significant differences were found between male and female rats in EA intervention for plasma LDL-C and HDL-C levels.EA intervention can effectively regulate obesity rats' Lee's index and plasma TG, TC, LDL-C and HDL-C levels. The effects are different in male and female rats.

Phosgene is a poorly water-soluble gas penetrating the lower respiratory tract which can induce acute lung injury characterized by a latent phase of fatal pulmonary edema. Pulmonary edema caused by phosgene is believed to be a consequence of oxidative stress and inflammatory responses. Ethyl pyruvate (EP) has been demonstrated to have anti-inflammatory and anti-oxidative properties in vivo and in vitro. The potential therapeutic role of EP in phosgene-induced pulmonary edema has not been addressed so far. In the present study, we aim to investigate the protective effects of EP on phosgene-induced pulmonary edema and the underlying mechanisms. Rats were administered with EP (40 mg kg(-1)) and RAW264.7 cells were also incubated with it (0, 2, 5 or 10 µm) immediately after phosgene (400 ppm, 1 min) or air exposure. Wet-to-dry lung weight ratio (W:D ratio), nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production, cyclooxygenase2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, and mitogen-activated protein kinases activities (MAPKs) were measured. Our results showed that EP treatment attenuated phosgene-induced pulmonary edema and decreased the level of NO and PGE(2) dose-dependently. Furthermore, EP significantly reduced COX-2 expression, iNOS expression and MAPK activation induced by phosgene. Moreover, specific inhibitors of MAPKs reduced COX-2 and iNOS expression induced by phosgene. These findings suggested that EP has a protective role against phosgene-induced pulmonary edema, which is mediated in part by inhibiting MAPK activation and subsequently down-regulating COX-2 and iNOS expression as well as decreasing the production of NO and PGE(2).

To observe the effect of otopoint-needle embedment on migraine and to explore its underlying mechanism.Ninety-two migraine outpatients were equally randomized into acupuncture group and medication group. For patients of the acupuncture group, one sterilized intradermal needle was inserted into E (MA-AT)-point and advanced toward Nie (temple-point, MA-AT), then another inserted into Nie (Temple-point, MA-AT) and advanced toward Zhen (occipus-point, MA-AT), followed by fixing the needles with a piece of adhesive-tape respectively, combined with Wei (stomach-point, MA-IC), Jiaogan (sympathesis-point, MA-AH 7) for migraine patients with nausea and vomiting, and with Yan (eye-point, MA-L 1) for those with phengophobia. Plasma serotonin (5-HT) contents before and after the treatment were detected by fluorescence spectrophotometry.In comparison with the pre-treatment, the synthetic scores of two groups one month and 6 months after the treatment were decreased significantly (P0.01). The integrative sores of the acupuncture group one month and 6 months after the treatment were significantly lower than those of the medication group (P0.01). The total effective rates of recent efficacy and long-term efficacy of the acupuncture group were 82.61% (38/46) and 83.72% (36/43) respectively, being significantly higher than those [67.39% (31/46) and 57.14% (24/42)] of the medication group. After the treatment, plasma 5-HT content of the acupuncture group was remarkablely higher than that of the medication group (P0.05).Otopoint-needle implant can effectively relieve headache in migraine patients and upregulate plasma 5-HT level.

To investigate the effects of anluohuaqianwan on experimental hepatic fibrosis induced by dimethyl nitrosamine (DMN) in rats.36 male SD rats were randomly dividied into three groups: model group, normal group, anluohuaqianwan group. The rats in the three groups were treated with DMN daily for 4 weeks. The liver function was detected using auto biochemistry analyzer, the serum HA, LN, IV-C, PIIIP were detected by immunoradiometry, the histopathology was observed in the left liver lobe after HE staining, the expression of matrix metalloproteinase-2 (MMP-2) in liver tissue were detected by immunohistochemistry.The serum levels of ALT, AST, ALP, TP, ALB and the contents of HA, LN, IV-C in model group were significantly increased compared to these in the normal group (P less than 0.01). The serum levels of ALT, AST and the contents of HA in anluohuaqianwan group were significantly lower than those in the model group (P less than 0.01). The liver MMP-2 in the model group was significantly increased compared to that in the normal group (P less than 0.05). The expression of MMP-2 in liver tissue of model group was lower than that in the anluohuaqianwan group (P less than 0.05).Anluohuaqianwan can inhibit liver fibrosis in rats induced by DMN.

To observe the effect of electroacupuncture (EA) on hippocampal apoptosis and learning-memory ability in vascular dementia (VD) mice so as to investigate its underlying mechanism in the treatment of VD patients.Kunming mice were randomly divided into sham-operation (sham, n = 13), model (n = 15), EA (n = 16) and Nimodipine (intragastric gavage, 30 mg/kg for 15 days, n = 15) groups. VD model was duplicated by occlusion of bilateral carotid arteries and reperfusion. EA (2-80 Hz, 2 mA) was applied to "Dazhui" (GV 14), "Baihui" (GV 20), "Geshu" (BL 17) and "Zusanli" (ST 36) for 10 min, once daily for 15 days. Step-down and step-up tests were performed to assess the animal's memory and learning abilities separately; and the terminal deoxynucleotidyl transferase-mediated dUTP-nick end-labeling (TUNEL) method was used to display the apoptotic cells of the hippocampus tissue.In comparison with sham group, the animals' reaction time upon electric shock stimulation, both step-up and step-down error times, and hippocampal apoptosis number in model, EA and Nimodipine groups increased significantly (P0.01), while the reaction latency upon electric shock of model, EA and Nimodipine groups decreased significantly (P0.01). Compared with model group, the reaction time, both step-up and step-down error times and hippocampal apoptosis number of both EA and Nimodipine groups decreased significantly (P0.05, P0.01); while the reaction latency of EA and Nimodipine groups increased considerably (P0.01). Comparison between EA and Nimodipine groups showed that the reaction latency of EA group was obviously longer than that of the later group (P0.01), and the step-down error times of EA group was markedly lower than that of Nimodipine group (P0.05). No significant differences were found between these two groups in other indexes (P0.05).EA can ameliorate VD mice's learning-memory ability, which may be closely related to its effect in reducing hippocampal apoptosis.

To study the liver histopathological features of chronic HBV carriers and inactive HBsAg carriers.Liver biopsies were performed on 189 chronic HBV carriers and 30 inactive HBsAg carriers (219 cases in total). All of them had a normal serum ALT value; they were then followed-up for more than 6 months. HBsAg and HBcAg were detected by immunohistochemistry. The circulating HBV DNA loads and serologic markers of HBV were examined at the same time. Grades of liver necrosis/inflammation and fibrosis were compared between the patients regarding their HBV DNA positivity or negativity. The relationships between the HBeAg positivity and degrees of liver histological changes were evaluated. The grades of liver necrosis/inflammation and fibrosis were compared between three age groups: younger than 18 years, 18-40, and older than 40 years.Two hundred eight carriers of the total 219 (95.0%) had histological liver changes. Fifty percent (104/208) of them had mild histological changes (G0-1/S0-1), while more severe changes (G3-4 and/or S3-4) were found in 18 out of the 208. There were no significant differences in the grades of liver necrosis/inflammation and fibrosis between the chronic HBV carriers and the inactive HBsAg carriers. Among the serologic HBV DNA positive carriers, hepatic fibrosis was more severe in the HBeAg negative group than in the positive group (chi2 = 9.551, P = 0.008), but no differences of the necrosis/inflammation grades were seen between the two groups. The rate of severe fibrosis (S3-4) was 21.1% in those carriers older than 40 years but was 7.7% in patients younger than 18 years. However, no statistically significant differences in degrees of liver inflammation and fibrosis were found among the three age groups. HBcAg positive rate was 100% in the liver tissues of all the chronic HBV carriers, but only in 33.3% in the inactive HBsAg carriers.The majority of our HBV carriers have liver inflammation and fibrosis. More severe histological changes were found in 8.65% of them. Liver fibrosis existed in the carriers with negative HBeAg and in those older than 40 years. HBcAg was found in hepatic tissues while their serological HBV DNA was negative.

To study apoptosis of pulmonary epithelial cells and endothelial cells in mice with pulmonary edema induced by phosgene exposure.Thirty-two mice were divided into normal group and phosgene group with 16 mice in each group. The mice in phosgene group were exposed to phosgene (11.9 mg/L) for 5 min and those in the control group to air. Four hours after exposure, alveolar type II cells were isolated and cultured to observe their apoptosis by electron microscope and flow cytometry. The lung tissues were also taken for DNA gel electrophoresis and TUNEL assay.Apoptotic bodies were observed in alveolar type II cells under electron microscope in phosgene group, which had higher cell apoptosis rate than the control group [(40.26+/-7.74)% vs (1.58+/-1.01)%, P0.001] as determined by flow cytometry. Ladder-like DNA fragmentation pattern was observed in DNA gel electrophoresis in phosgene group with apoptosis of the pulmonary epithelial and endothelial cells observed by TUNEL.Phosgene can induce pulmonary epithelial and endothelial cell apoptosis in mice, suggesting that the mechanism of phosgene-induced pulmonary edema involves apoptosis of the lung cells.

To study the apoptosis of alveolar type II cells, alterations of vascular endothelial growth factor (VEGF), VEGF receptor (Flt1) in serum and lung and expression of VEGF mRNA in lung in pulmonary edema mice induced by phosgene.Twenty-six BALB/C mice were randomly divided into 2 groups: control group, exposed group (13 mice in each group). Mice of exposed group were intoxicated by inhalation of phosgene 11.9 mg/L for 5 minutes. Mice of control group were treated as the same way by inhalation of air. Isolation of mice alveolus type II cells 4 h after intoxication was carried out to observe their apoptosis under electron microscope. Contents of VEGF and Flt1 in lung and serum by ELISA, and expression of VEGF mRNA were determined.Alveolar type II cells were identified by tannic acid staining and electron microscopy. After exposed to 11.9 mg/L of phosgene for 5 minutes, the apoptotic body in alveolus type II cells was found in exposed group. The contents of VEGF in serum and lung and Flt1 in lung of exposed mice [(134.07 +/- 120.26), (477.76 +/- 98.06), (1,2818.48 +/- 2,304.15) pg/ml] were significantly lower than those of control group [(445.57 +/- 173.30), (1,026.87 +/- 474.56), (21,976.51 +/- 7,421.01) pg/ml, P0.05] but the content of Flt1 in serum [(2,369.56 +/- 381.70) pg/ml] was higher than that in control group [(1,898.00 +/- 453.69) pg/ml, P0.05]. The expression of VEGF mRNA in pulmonary edema mice was decreased.Phosgene can induce apoptosis of alveolar type II cells, and decrease in the content of VEGF and Flt1, and expression of VEGF mRNA in lung.

To observe the significance of ricin (RT) with chemical nidification to reduce the hepatotoxicity in mice and its anticancer effect.Mice were exposed to RT and RT-PDP [ricin chemically modified by N-succinimidyl 3-(2-pyridyldithio)-propionate, (SPDP)] respectively, and their serum activity of glutathione-S-transferase (GST) and liver glutathione (GSH) content were determined. The ultramicro-structure under electron microscope was also observed.The GST activity increased with doses, and the increase in ricin group was higher than that in RT-PDP group; the activities of GST in RT group at 12.5, 15.0 micro g/kg [(93.65 +/- 12.30), (153.71 +/- 26.64) IU/L respectively] were higher than those in RT-PDP group [(62.97 +/- 11.22), (78.20 +/- 15.71) IU/L] (P0.05). The contents of GSH were decreased with doses; but the contents of GSH in RT-PDP group at 2.5, 5.0, 7.5, 10.0, 15.0 micro g/kg [(6.34 +/- 1.43), (4.14 +/- 1.82), (3.54 +/- 0.64), (2.73 +/- 1.82), (1.82 +/- 0.62) micro mol/L respectively] were still higher than those in RT group [(3.53 +/- 0.95), (2.12 +/- 0.54), (1.82 +/- 0.71), (1.52 +/- 0.34), (0.81 +/- 0.36) micro mol/L] (P0.01). Electron microscopic examination showed that the injury of liver cells in RT group was more severe than that in RT-PDP group.The hepatotoxicity of ricin in mice may be reduced by chemical modification.

To study the relationship of the long-term incidence rate of benign prostate hyperplasia (BPH) after vasectomy to age and time after operation.Questionnaire survey, physical examination, and ultrasonography were conducted among 850 healthy males, aged 55 - 76 who underwent vasectomy more than 20 years before and 390 healthy males, aged 53 - 75, who had not undergone vasectomy in Yichang, Hubei province, selected by randomized cluster sampling. The data such as international prostate symptom scoring (IPSS), quality of life (QOL) index, maximum urine flow rate (MFR), and average flow rate (AFR), digital rectal examination (DRE), volume of prostate gland (V), and residual urine (R) were analyzed.The general BPH incidence rate of the vasectomy group was 10.8%, significantly lower than that of the control group (40.8%, P0.01). The age-specific BPH incidence rates in the vasetomy group were all significantly lower than those of the control group, especially that of the subjects aged over 70 (10.8% vs. 56.5%, P0.001). The BPH incidence rate of those who had undergone vasectomy 25 years or over before was 7.8%, significantly lower than that of those who had undergone vasectomy 20 years before (13.2%, P0.05), but not significantly difference from that of those who had undergone vasectomy 30 years or over before (5.5%, P0.05). The volumes of prostate gland in all age subgroups were significantly smaller in the vasectomy group than in the control group.Vasectomy reduces the incidence rate of BPH significantly.

To investigate the possibility and efficacy of vasoligation and deferent vein ligation in treating and preventing benign prostatic hyperplasia(BPH).40 male pooches were divided into A, B, C and D groups randomly. Each group has 10 pooches group A and B were made into the model of BPH. Two years later, the pooches in group A and C accepted the operation of vasoligation and deferent vein ligation on both sides. Group B and D only accepted the operation of dissection and relieving its deferent duct. Then continuing to feed the 40 pooches after the operation, they were killed another two years later. After taking out their prostates and calculating their volume and weight, the sections of the prostates were checked by microscope to observe their histology and pathology changes.There were significant differences of weight and volume as well as the changes of histology and pathology between group A and group C (P0.01). It was the same with group B and D (hyperplasia changes).Vasoligation and deferent vein ligation before benign prostatic hyperplasia at pooches grow-up stage can reduce the extent of BPH at old age, and treatment after benign prostatic hyperplasia can make prostatic tissue appear different degrees of atrophy.