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Marine litter impacts oceans and affects marine organisms, representing a potential threat for natural stocks of pelagic fish species located at the first levels of the marine food webs. In 2013-2014, on a seasonal basis, marine litter and microplastics in stomach contents from Sardinia pilchardus and Engraulis encrasicolus were evaluated. Selected species are plankitivores of great ecological and commercial importance in the Adriatic Sea. Collected data were correlated to possible factors able to affect ingested levels as well as species, season of sampling, biometry and sex of animals. Almost all tested samples (80 organisms for each species) contained marine litter (over 90% of samples from both species) and also microplastics; while any meso- or macroplastics were recorded. On average, recorded items were as follows: 4.63 (S. plichardus) and 1.25 (E. encrasicolus) per individual. Sardines evidenced a higher number of microplastics characterised by a smaller size than those recorded in anchovies. For sardines, sex, Gastro Somatic Index and sampling season showed negligible effects on the number of ingested litter; conversely, anchovies showed differences related with both sex of animals and dominant colour of ingested materials with prevalence for black and blue colours.

The occurrence of the protozoan parasites Toxoplasma gondii and Cyclospora cayetanensis was molecularly investigated in the hemolymph, gills, stomach, hepatopancreas and gonads of the eleven invasive Atlantic blue crab Callinectes sapidus from the Lesina Lagoon (Mediterranean Sea). Out of 11 blue crabs, 6 (54.5%) and 4 (36.4%) were found positive to T. gondii and C. cayetanenis, respectively; parasites were found only in the six females analysed, while the remaining five males resulted negative. Moreover, out of 55 tissues samples, 7 (12.7%) and 8 (14.5%) were positive to T. gondii and C. cayetanensis, respectively with hemolymph and gills being the most infected tissues. This is the first report of the presence of protozoan pathogens in wild crab species collected from a Mediterranean lagoon. The present results may provide a baseline reference on microbial infection in the species for invaded Mediterranean waters, and on the potential health risks related with its consumption if eaten raw.

Changes in environmental stressors inevitably lead to an increasing need for innovative and more flexible monitoring tools. The aim of this work has been the characterization of the motility pattern of the cryopreserved sea bream semen after exposure to a dumpsite leachate sample, for the identification of the best representative parameters to be used as endpoints in an ecotoxicological bioassay. Sperm motility has been evaluated either by visual and by computer-assisted analysis; parameters concerning motility on activation and those describing it in the times after activation (duration parameters) have been assessed, discerning them in terms of sensitivity, reliability and methodology of assessment by means of multivariate analyses. The EC50 values of the evaluated endpoints ranged between 2.3 and 4.5 ml/L, except for the total motile percentage (aTM, 7.0 ml/L), which proved to be the less sensitive among all the tested parameters. According to the multivariate analyses, a difference in sensitivity among "activation" endpoints in respect of "duration" ones can be inferred; on the contrary, endpoints seem to be equally informative either describing total motile sperm or the rapid sub-population, as well as the assessment methodology seems to be not discriminating. In conclusion, the CRYO-Ecotest is a multi-endpoint bioassay that can be considered a promising innovative ecotoxicological tool, characterized by a high plasticity, as its endpoints can be easy tailored each time according to the different needs of the environmental quality assessment programs. (C) 2015 Elsevier Inc. All rights reserved.

Good results have been reported after both primary repair and conservative management of grade III ankle sprains. This prospective study found no advantage of operative treatment compared to taping; no significant differences were found between the groups with regard to objective or subjective stability, functional scores, or the overall result.

The retrospective study concerns use of the AO method for the treatment of 75 ankle fractures between 1990 and 1995. After a mean follow-up of 5 years and 7 months the Cedell criteria were used for a subjective evaluation of results. Good results were obtained in 53 out of 75 patients submitted to surgery (70%), with a significant difference between uni-malleolar fractures and bi- and tri-malleolar fractures. Dorsal flexion measured an average of 24 degrees (80% of the healthy contralateral side), plantar flexion 36 degrees (90% of the healthy side). The presence of a fracture-dislocation considerably worsens prognosis because of the cartilaginous damage produced. The risk of poor results increases considerably when reduction of fracture is insufficient or absent. Post-traumatic arthrosis constitutes a common complication (25%) and it particularly affects females aged between 45 and 65 years, as a result of the presence of post-menopausal osteoporosis. Accurate reduction, fracture stabilization, sex and age constitute essential elements for satisfactory final results.

A total of 15 patients affected with Perthes' disease that occurred during adolescent were followed-up in order to study progression of the disease, and to identify the "risk factors" capable of influencing the future of the lesion. Age of onset was on the average 11 years (10-13 years), age at follow-up was 38 years (20-56 years). On diagnosis, 2 hips were classified as Catterall group II, 8 as group III, 6 as group IV. Treatment was conservative in 11 patients and surgical in 4 (valgus osteostomy). A total of 88% of the hips healed with deformity to a greater or lesser degree of the femoral epiphysis (Stulberg III-IV and V). The most significant prognostic factors that emerged from the present study were the early closing of growth plate (femoral and triradiate), disorders in growth of the proximal femur, acetabular roof insufficiency, and changes in the acetabulum.

Long-term follow-up (5 years and 7 months) was obtained for the results of core decompression in 18 patients (20 hips) affected with ischemic necrosis of the femoral head at Ficat stages I and IIA. Two methods were used to diagnose osteonecrosis: bone scan, and MRI. Progression of the disease was monitored by annual MRI. The persistence of pain, progression of the disease in the Ficat scale, the use of total arthroplasty, were considered to be signs of failure. Core decompression was favorable in 16 hips, there was progression of the disease in 4. Good results were observed in 90% of the stage I necroses and in 70 of the stage IIA necroses. The results of the present study suggest bone reaming as the treatment of choice in initial stages of necrosis. Thus, the need for arthroplasty in young patients is delayed.

A case of isolated dorsal dislocation of the fifth carpometacarpal joint treated conservatively is reported. The mechanism is thought to be a force hitting the head of the fifth metacarpal in a dorso-palmar direction, along the longitudinal axis of the metacarpal. Both clinical and radiological results 1 year after injury are satisfactory.

We reviewed 36 patients who had undergone reconstructive surgery of the ligaments of the lateral compartment of the ankle between 1981 and 1986. The follow-up period averaged 6 years (range, 3-9 years). Ligamentous lesion was diagnosed by clinical examination and contact thermography. Eighty-six percent of the subjects reviewed achieved a good result, resuming sports activity and regaining full joint stability. Reports in the literature are conflicting: good results have been achieved by both conservative treatment and reconstructive surgery. We are convinced that surgery offers young athletes a better chance of regaining joint stability because it is the only treatment that promotes anatomical healing of the ligaments without lengthening or loss of strength. Contact thermography is an important means of diagnosis because of its few limitations and low cost.

Fifty-three patients treated between 1979 and 1986 with high tibial osteotomy for varus osteoarthritic knee are evaluated. The average follow-up was 6 years and 8 months (range 3-10 years). All of the osteotomies were stabilized using Weber's method. The purpose of the operation was to realign the mechanical axes with overcorrection of 3 degrees. Results were good in 71% of the patients, and 85% claimed to be satisfied with the operation. The preoperative varus, 9.5 degrees on average, was corrected to 2-degree valgus; the deformity was exactly corrected in 59% of the cases, undercorrected in 9%, and overcorrected in 32%. The corrections slowly deteriorated over time. The correlation between the results and the stage of the arthritis, the degree of deformity, and the alignment of the mechanical axes showed that the best results were obtained in monocompartmental lesions with a varus deformity of less than 10 degrees and in osteotomies resulting in an average postoperative mechanical axis of 184.5 degrees. Weber's method has undeniable advantages over other methods, such as stable internal fixation, rapid healing, early restoration of articular function and resumption of weight-bearing.

Three cases of demoplastic fibroma of bone are presented. Thirty six cases already reported in the world literature are reviewed, making the present total thirty nine cases. The extreme rarity of the tumor is emphasised, and its clinical, radiographic, morphological and pathological aspects discussed. Though the absence of metastases or histologically abnormal cells attest to its benign nature it has a definite tendency to local recurrence. Differential diagnosis and treatment are discussed.

The authors present a case of intraosseus lipoma seen at the Orthopaedic Clinic of the University of Bari, bringing the total in the world literature to thirty cases. The tumour is a benign primary neoplasm of bone, made up of mature adipose tissue containing atrophic bone trabeculae. This affection is a definite entity quite distinct from parosteal lipomas arising in periosteum, fasciae and muscles which only affect the bone secondarily by compression or direct invasion. Emphasizing the extreme rarity of the tumour, the authors discuss its clinical, radiological, anatomical and pathological features. The differential diagnosis is with certain dysplasic and neoplastic lesions of bone such as: bone cyst, non-ossifying fibroma, aneurismal cyst, monostotic fibrous dysplasia, and solitary myeloma. Complete removal is followed by cure.

This work compares the results of 2 different techniques of anterior cruciate reconstruction performed by 2 surgeons with the same rehabilitation program and same criteria of results evaluation. Forty-five patients were operated on using semitendinous tendon autograft, (average followup, 8 years 4 months), and 30 patients using bone patellar tendon bone autograft (average followup, 7 years 5 months). Functional results were evaluated using the Lysholm and Tegner rating systems, and clinical laxity or stability were evaluated using the Lachmann and Jerk tests. Articular stability and function were significantly better in the patients operated on using bone patellar tendon bone autograft.

The width of the weight-bearing surface (WBS) of the acetabulum and its obliquity; height, width, opening angle and alpha angle of the gothic arch; diameter of femoral head; the angle between the center of the femoral head and the most lateral point of the acetabulum (CE angle); neck-shaft angle (CCD angle); offset; angle of aperture, surface area, and volume of the spherical sector; radius and height of the spherical bowl; and distance from teardrop were assessed to study mechanically normal and frankly osteoarthritis hips. There appears to be a direct correlation between primary alterations of the above variables and development of osteoarthritis of the hip.